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Information on EC 2.5.1.15 - dihydropteroate synthase and Organism(s) Arabidopsis thaliana and UniProt Accession Q1ENB6

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IUBMB Comments
The enzyme participates in the biosynthetic pathways for folate (in bacteria, plants and fungi) and methanopterin (in archaea). The enzyme exists in varying types of multifunctional proteins in different organisms. The enzyme from the plant Arabidopsis thaliana also harbors the activity of EC 2.7.6.3, 2-amino-4-hydroxy-6-hydroxymethyldihydropteridine diphosphokinase , while the enzyme from yeast Saccharomyces cerevisiae is trifunctional with the two above mentioned activities as well as EC 4.1.2.25, dihydroneopterin aldolase .
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This record set is specific for:
Arabidopsis thaliana
UNIPROT: Q1ENB6
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Word Map
The taxonomic range for the selected organisms is: Arabidopsis thaliana
The enzyme appears in selected viruses and cellular organisms
Synonyms
dihydropteroate synthase, pfdhps, pvdhps, dihydropteroate synthetase, folp1, folp2, pvhppk-dhps, pppk-dhps, rv1207, hppk-dhps, more
SYNONYM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
7,8-dihydropteroate synthase
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dihydropteroate synthase
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7,8-dihydropteroate synthase
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-
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7,8-dihydropteroate synthetase
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-
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7,8-dihydropteroic acid synthetase
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-
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DHPS
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-
-
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dihydropteroate diphosphorylase
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-
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dihydropteroate synthetase
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-
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dihydropteroic synthetase
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-
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synthase, dihydropteroate
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-
-
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REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
aryl group transfer
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-
-
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PATHWAY SOURCE
PATHWAYS
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-, -
SYSTEMATIC NAME
IUBMB Comments
(7,8-dihydropterin-6-yl)methyl diphosphate:4-aminobenzoate 2-amino-4-hydroxy-7,8-dihydropteridine-6-methenyltransferase
The enzyme participates in the biosynthetic pathways for folate (in bacteria, plants and fungi) and methanopterin (in archaea). The enzyme exists in varying types of multifunctional proteins in different organisms. The enzyme from the plant Arabidopsis thaliana also harbors the activity of EC 2.7.6.3, 2-amino-4-hydroxy-6-hydroxymethyldihydropteridine diphosphokinase [4], while the enzyme from yeast Saccharomyces cerevisiae is trifunctional with the two above mentioned activities as well as EC 4.1.2.25, dihydroneopterin aldolase [3].
CAS REGISTRY NUMBER
COMMENTARY hide
9055-61-2
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SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
2-amino-4-hydroxy-6-hydroxymethyl-7,8-dihydropteridine diphosphate + 4-aminobenzoate
diphosphate + 7,8-dihydropteroate
show the reaction diagram
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-
-
?
NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
2-amino-4-hydroxy-6-hydroxymethyl-7,8-dihydropteridine diphosphate + 4-aminobenzoate
diphosphate + 7,8-dihydropteroate
show the reaction diagram
-
-
-
?
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
-
SwissProt
Manually annotated by BRENDA team
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
UNIPROT
ENTRY NAME
ORGANISM
NO. OF AA
NO. OF TRANSM. HELICES
MOLECULAR WEIGHT[Da]
SOURCE
SEQUENCE
LOCALIZATION PREDICTION?
FOLC_ARATH
484
0
54110
Swiss-Prot
other Location (Reliability: 3)
PROTEIN VARIANTS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
additional information
bifunctional enzyme, mitochondrial enzyme ubiquitously expressed, cytosolic enzyme only in reproductive tisse, construction of a chimeric protein containing the first 224 amino acids of cytosolic DHPS fused to GFP, expression in Arabidopsis thaliana, Arabidopsis thaliana mutant lacking DHPS indicates a role of DHPS in seed stress
PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
overexpression of the N-terminal His6 fusion protein in Escherichia coli, coexpression with chaperone genes to increase solubility
CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
expression in Escherichia coli as N-terminal His6 fusion
expression in Saccharomyces cerevisiae Fol1 knockout Y26466
REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Storozhenko, S.; Navarrete, O.; Ravanel, S.; De Brouwer, V.; Chaerle, P.; Zhang, G.F.; Bastien, O.; Lambert, W.; Rebeille, F.; Van Der Straeten, D.
Cytosolic hydroxymethyldihydropterin pyrophosphokinase/dihydropteroate synthase from Arabidopsis thaliana: a specific role in early development and stress response
J. Biol. Chem.
282
10749-10761
2007
Arabidopsis thaliana (Q1ENB6)
Manually annotated by BRENDA team