BRENDA - Information on EC 2.4.99.9 - lactosylceramide alpha-2,3-sialyltransferase

2.4.99.9

-

lactosylceramide alpha-2,3-sialyltransferase

CMP-N-acetylneuraminate + beta-D-galactosyl-(1->4)-beta-D-glucosyl-(1<->1)-ceramide = CMP + alpha-N-acetylneuraminyl-(2->3)-beta-D-galactosyl-(1->4)-beta-D-glucosyl-(1<->1)-ceramide

-

glycosyl group transfer

-

Glycosphingolipid biosynthesis - ganglio series

00604

-

Metabolic pathways

01100

-

CMP-N-acetylneuraminate:beta-D-galactosyl-(1->4)-beta-D-glucosyl-(1<->1)-ceramide alpha-(2->3)-N-acetylneuraminyltransferase

Lactose cannot act as acceptor.

beta-galactoside alpha-2,3-sialyltransferase 5

Mus musculus

O88829

-

723516

CMP-acetylneuraminate-lactosylceramide-sialyltransferase

-

CMP-acetylneuraminic acid:lactosylceramide sialytransferase

-

CMP-N-acetylneuraminic acid:lactosylceramide 2,3-sialyltransferase

Mus musculus

-

702982

CMP-N-acetylneuraminic acid:lactosylceramide alpha2,3-sialyltransferase

Homo sapiens

-

705676

CMP-NeuAc:lactosylceramide alpha-2,3-sialyltransferase

-

CMP-NeuAc:lactosylceramide alpha2,3-sialyltransferase

Mus musculus

-

660348

CMP-sialic acid:lactosylceramide-sialyltransferase

-

cytidine monophosphoacetylneuraminate-lactosylceramide alpha-2,3-sialyltransferase

-

cytidine monophosphoacetylneuraminate-lactosylceramide sialyltransferase

-

ganglioside GM3 synthase

Mus musculus

-

706549

Ganglioside GM3 synthase

-

ganglioside GM3 synthetase

-

GM3 synthase

-

GM3 synthase

Homo sapiens

-

657703, 658723, 658853, 672358, 674638, 675938, 701568, 703916, 705616, 705676, 721691

GM3 synthase

Homo sapiens

Q9UNP4

-

658140, 672358, 721545, 721922

GM3 synthase

Mus musculus

-

658868, 660348, 673509, 702982, 705616

GM3 synthetase

-

GM3S

-

hST3Gal V

-

Laccer sialyltransferase

Mus musculus

-

658868

lactosylceramide alpha2,3-sialyltransferase

-

SAT-1

-

SAT-I

-

SAT-I

-

SAT1

-

Sia-T1

Homo sapiens

-, Q9UNP4

-

sialyltransferase-1

-

Siat9

-

ST3Gal V

-

672358, 701568, 705676

ST3Gal V

Homo sapiens

Q9UNP4

-

ST3Gal V

-

ST3Gal5

-

ST3Gal5

O88829

-

723516

ST3GalV

Mus musculus

-

673509

125752-90-1

-

15 days old embryo

-

7-11 days old embryo

-

-

636877, 637739, 657703, 658723, 658853, 674638, 675938, 688984, 701568, 703916, 705616, 705676, 721691

-

Homo sapiens

-

658140, 721545, 721922

Q9UNP4

SwissProt

Homo sapiens

5 different isozymes/mRNA variants of different lengths, overview

672358

-

Homo sapiens

SIAT9; 5 different isozymes/mRNA variants of different lengths, overview; ST3GAL5, SIAT9; 5 different isozymes/mRNA variants of different lengths, overview

SwissProt

-

-

-

489583, 638793, 660348, 702982, 705616, 706549

-

Mus musculus

-

658868, 673509, 723516

O88829

Uniprot

Oreochromis mossambicus

cichlid fish

636888

-

Rattus norvegicus

-

636877, 636888, 637737, 637742, 638779, 638780, 638781, 638782, 638783, 638784, 638788, 638791, 638792

-

Rattus norvegicus

female Wistar

637736

-

malfunction

Mus musculus

-

in SAT-I null mice, hearing ability is impaired at the onset of hearing and is completely lost by 17 days after birth, showing a deformity in hair cells in the organ of Corti, by 2 months of age, the organ of Corti selectively and completely disappears without effect on balance or motor function or in the histology of vestibule. Lack of brainstem auditory-evoked potentials (BAEPs) in SAT-I null mice due to selective degeneration of the organ of Corti. SAT-I null mice maintain the function of stria vascularis. Defect of hearing ability of SAT-I null mice can be attributed to the functional disorganization of the organ of Corti, and the expression of gangliosides, especially GM3, during the early part of the functional maturation of the cochlea can be essential for the acquisition and maintenance of hearing function

706549

physiological function

Mus musculus

-

GM3S knockdown significantly suppresses expression of the ganglioside GM3 and the more complex ganglioside GD3. GM3 synthase silencing in 4T1 cells significantly inhibits cell migration, invasion and anchorage-independent growth in vitro, and lung metastasis in vivo. Overexpression of GM3 synthase in nonmetastatic 67NR cells significantly induces cell migration and anchorage-independent growth. GM3S promotes breast cancer cells migration and invasion through the phosphoinositide-3 kinase/Akt pathway, whereas anchorage-independent growth caused by GM3S is not associated with the phosphoinositide-3 kinase/Akt pathway

702982

physiological function

Homo sapiens

-

overexpression of GM3 synthase in A2780 cells consistently results in elevated ganglioside (GM3, GM2 and GD1a) levels. GM3 synthase overexpressing cells have a growth rate similar to wild-type cells, but show a strongly reduced in vitro cell motility accompanied by reduced levels of the epithelial-mesenchymal transition marker alpha smooth muscle actin. Caveolin-1 is markedly upregulated in GM3 synthase overexpressing cells. Higher levels of the inactive form of c-Src are present in GM3 synthase overexpressing cells, associated with a ganglioside- and caveolin-rich detergent insoluble fraction

703916

physiological function

Mus musculus

-

M1-SAT-I, M2-SAT-I, and M3-SAT-I isoforms are produced by leaky scanning from mRNA variants of SAT-Ia and SAT-Ib. SAT-I leads to a reduction in GM1b and an increase in GM3, GM1, and GD1a levels. Ganglioside levels in ks-M1-SAT-I-transfected cells are similar to those in ks-M2-SAT-I- or ks-M3-SAT-I-transfected cells. Introduction of ks-M1-SAT-I or ks-M2-SAT-I into CHO cells leads to the production of M3-SAT-I by leaky scanning. M1-SAT-I and M3-SAT-I have a long half-life relative to M2-SAT-I

705616

physiological function

Homo sapiens

-

the enzyme catalyzes ganglioside GM3 biosynthesis in ARPE-19 human retinal pigment epithelial cells

721922

CMP-N-acetylneuraminate + 1-deoxy-1-cholesteryl (N-acetyl)-ethanolaminolactitol

CMP + ?

Rattus norvegicus

-

115% of activity with lactosylceramide

638792

-

?

CMP-N-acetylneuraminate + 1-deoxy-1-cholesterylethanolaminolactitol

CMP + ?

Rattus norvegicus

-

28% of activity with lactosylceramide

638792

-

?

CMP-N-acetylneuraminate + 1-deoxy-1-cholesterylphospho (N-acetyl)-ethanolaminolactitol

CMP + ?

Rattus norvegicus

-

151% of activity with lactosylceramide

638792

-

?

CMP-N-acetylneuraminate + 1-deoxy-1-cholesterylphosphoethanolaminolactitol

CMP + ?

Rattus norvegicus

-

60% of activity with lactosylceramide

638792

-

?

CMP-N-acetylneuraminate + 2,3-dicholesteryl-1-beta-lactosylglycerol

?

Rattus norvegicus

-

113% of activity with lactosylceramide

638792

-

?

CMP-N-acetylneuraminate + 2-cholesteryl-1-beta-lactosylglycerol

?

Rattus norvegicus

-

138% of activity with lactosylceramide

638792

-

?

CMP-N-acetylneuraminate + 3-cholesteryl-1-beta-lactosylglycerol

?

Rattus norvegicus

-

163% of activity with lactosylceramide

638792

-

?

CMP-N-acetylneuraminate + asialoGM1

CMP + GM1

Homo sapiens

-, Q9UNP4

-

672358

-

?

CMP-N-acetylneuraminate + asialoGM1

?

Homo sapiens

-

27.7% relative activity to lactosylceramide

705676

-

?

CMP-N-acetylneuraminate + asialoGM2

CMP + GM2

Homo sapiens

-, Q9UNP4

-

672358

-

?

CMP-N-acetylneuraminate + beta-D-galactosyl-1,3-N-acetylgalactosamin-1,4-beta-D-galactosyl-1,4-beta-D-glucosylceramide

CMP + ?

Rattus norvegicus

-

i.e. asialo-ganglioside GM1, sialylated at about 84% the rate of lactosylceramide

638780

-

?

CMP-N-acetylneuraminate + beta-D-galactosyl-1,3-N-acetylgalactosamin-lactosylceramide

CMP + ?

Rattus norvegicus

-

14% of activity with lactosylceramide

638783

-

?

CMP-N-acetylneuraminate + beta-D-galactosyl-1,4-beta-D-glucosylceramide

CMP + N-acetylneuraminyl-2,3-beta-D-galactosyl-1,4-beta-D-glucosylceramide

Mus musculus

-

723516

-

?

CMP-N-acetylneuraminate + beta-D-galactosyl-1,4-beta-D-glucosylceramide

CMP + N-acetylneuraminyl-2,3-beta-D-galactosyl-1,4-beta-D-glucosylceramide

Gallus gallus

-

636879

i.e. ganglioside GM3

636879

?

CMP-N-acetylneuraminate + beta-D-galactosyl-1,4-beta-D-glucosylceramide

CMP + N-acetylneuraminyl-2,3-beta-D-galactosyl-1,4-beta-D-glucosylceramide

Gallus gallus

-

638776

i.e. ganglioside GM3

638776

?

CMP-N-acetylneuraminate + beta-D-galactosyl-1,4-beta-D-glucosylceramide

CMP + N-acetylneuraminyl-2,3-beta-D-galactosyl-1,4-beta-D-glucosylceramide

Mus musculus

-

638793

i.e. ganglioside GM3

638793

-

CMP-N-acetylneuraminate + beta-D-galactosyl-1,4-beta-D-glucosylceramide

CMP + N-acetylneuraminyl-2,3-beta-D-galactosyl-1,4-beta-D-glucosylceramide

Mus musculus

-

489583

i.e. ganglioside GM3

489583

?

CMP-N-acetylneuraminate + beta-D-galactosyl-1,4-beta-D-glucosylceramide

CMP + N-acetylneuraminyl-2,3-beta-D-galactosyl-1,4-beta-D-glucosylceramide

Homo sapiens

-

636877

i.e. ganglioside GM3

636877

?

CMP-N-acetylneuraminate + beta-D-galactosyl-1,4-beta-D-glucosylceramide

CMP + N-acetylneuraminyl-2,3-beta-D-galactosyl-1,4-beta-D-glucosylceramide

Homo sapiens

-

637739

i.e. ganglioside GM3

637739

?

CMP-N-acetylneuraminate + beta-D-galactosyl-1,4-beta-D-glucosylceramide

CMP + N-acetylneuraminyl-2,3-beta-D-galactosyl-1,4-beta-D-glucosylceramide

Rattus norvegicus

-

638792

i.e. ganglioside GM3

638792

-

CMP-N-acetylneuraminate + beta-D-galactosyl-1,4-beta-D-glucosylceramide

CMP + N-acetylneuraminyl-2,3-beta-D-galactosyl-1,4-beta-D-glucosylceramide

Rattus norvegicus

-

636877

i.e. ganglioside GM3

636877

?

CMP-N-acetylneuraminate + beta-D-galactosyl-1,4-beta-D-glucosylceramide

CMP + N-acetylneuraminyl-2,3-beta-D-galactosyl-1,4-beta-D-glucosylceramide

Rattus norvegicus

-

636888

i.e. ganglioside GM3

636888

?

CMP-N-acetylneuraminate + beta-D-galactosyl-1,4-beta-D-glucosylceramide

CMP + N-acetylneuraminyl-2,3-beta-D-galactosyl-1,4-beta-D-glucosylceramide

Rattus norvegicus

-

637736

i.e. ganglioside GM3

637736

?

CMP-N-acetylneuraminate + beta-D-galactosyl-1,4-beta-D-glucosylceramide

CMP + N-acetylneuraminyl-2,3-beta-D-galactosyl-1,4-beta-D-glucosylceramide

Rattus norvegicus

-

637737

i.e. ganglioside GM3

637737

?

CMP-N-acetylneuraminate + beta-D-galactosyl-1,4-beta-D-glucosylceramide

CMP + N-acetylneuraminyl-2,3-beta-D-galactosyl-1,4-beta-D-glucosylceramide

Rattus norvegicus

-

637742

i.e. ganglioside GM3

637742

?

CMP-N-acetylneuraminate + beta-D-galactosyl-1,4-beta-D-glucosylceramide

CMP + N-acetylneuraminyl-2,3-beta-D-galactosyl-1,4-beta-D-glucosylceramide

Rattus norvegicus

-

638779

i.e. ganglioside GM3

638779

?

CMP-N-acetylneuraminate + beta-D-galactosyl-1,4-beta-D-glucosylceramide

CMP + N-acetylneuraminyl-2,3-beta-D-galactosyl-1,4-beta-D-glucosylceramide

Rattus norvegicus

-

638781

i.e. ganglioside GM3

638781

?

CMP-N-acetylneuraminate + beta-D-galactosyl-1,4-beta-D-glucosylceramide

CMP + N-acetylneuraminyl-2,3-beta-D-galactosyl-1,4-beta-D-glucosylceramide

Rattus norvegicus

-

638782

i.e. ganglioside GM3

638782

?

CMP-N-acetylneuraminate + beta-D-galactosyl-1,4-beta-D-glucosylceramide

CMP + N-acetylneuraminyl-2,3-beta-D-galactosyl-1,4-beta-D-glucosylceramide

Rattus norvegicus

-

638788

i.e. ganglioside GM3

638788

?

CMP-N-acetylneuraminate + beta-D-galactosyl-1,4-beta-D-glucosylceramide

CMP + N-acetylneuraminyl-2,3-beta-D-galactosyl-1,4-beta-D-glucosylceramide

Mesocricetus auratus

-

638785

i.e. ganglioside GM3

638785

?

CMP-N-acetylneuraminate + beta-D-galactosyl-1,4-beta-D-glucosylceramide

CMP + N-acetylneuraminyl-2,3-beta-D-galactosyl-1,4-beta-D-glucosylceramide

Oreochromis mossambicus

-

636888

i.e. ganglioside GM3

636888

?

CMP-N-acetylneuraminate + beta-D-galactosyl-1,4-beta-D-glucosylceramide

CMP + N-acetylneuraminyl-2,3-beta-D-galactosyl-1,4-beta-D-glucosylceramide

Rattus norvegicus

-

i.e. lactosylceramide, high specificity

638783

i.e. ganglioside GM3

638783

?

CMP-N-acetylneuraminate + beta-D-galactosyl-1,4-beta-D-glucosylceramide

CMP + N-acetylneuraminyl-2,3-beta-D-galactosyl-1,4-beta-D-glucosylceramide

Rattus norvegicus

-

preferred acceptors have the general structure beta1-O-ceramide, disaccharides are preferred to monosaccharide, no acceptors are fetuin, mucin, alpha1-acid glycoprotein, glycophorin or their respective asialo-derivatives, gangliosides GD1b, GM3, GM2, GM1 or GD1a

638780

i.e. ganglioside GM3

638780

?

CMP-N-acetylneuraminate + beta-D-galactosyl-1,4-beta-D-glucosylceramide

CMP + N-acetylneuraminyl-2,3-beta-D-galactosyl-1,4-beta-D-glucosylceramide

Rattus norvegicus

-

brain sialyltransferase shows high activity with d18:1-16:0, d18:1-22:1, and d18:0-18:0 lactosylceramide molecular species, specificity changes when the lipid composition of the neuronal microsomal membrane resembles that of liver Golgi membrane lipids

638791

i.e. ganglioside GM3

638791

-

CMP-N-acetylneuraminate + beta-D-galactosyl-1,4-beta-D-glucosylceramide

CMP + N-acetylneuraminyl-2,3-beta-D-galactosyl-1,4-beta-D-glucosylceramide

Rattus norvegicus Sprague-Dawley

-

636888

i.e. ganglioside GM3

636888

?

CMP-N-acetylneuraminate + beta-lactosylcholesterol

?

Rattus norvegicus

-

138% of activity with lactosylceramide

638792

-

?

CMP-N-acetylneuraminate + cholesteryl-beta-lactosylpropane-1,3-diol

?

Rattus norvegicus

-

143% of activity with lactosylceramide

638792

-

?

CMP-N-acetylneuraminate + galactosylceramide

CMP + ?

Rattus norvegicus

-

sialylated at about 40% the rate of lactosylceramide

638780

-

?

CMP-N-acetylneuraminate + galactosylceramide

CMP + alpha-N-acetylneuraminyl-2,3-beta-D-galactosyl-ceramide

Homo sapiens

-, Q9UNP4

-

672358

-

?

CMP-N-acetylneuraminate + galactosylceramide

?

Homo sapiens

-

30% relative activity to lactosylceramide

705676

-

?

CMP-N-acetylneuraminate + glucosylceramide

CMP + ?

Rattus norvegicus

-

sialylated at about 65% the rate of lactosylceramide

638780

-

?

CMP-N-acetylneuraminate + GM3

?

Homo sapiens

-

4.5% relative activity to lactosylceramide

705676

-

?

CMP-N-acetylneuraminate + lactosylceramide

CMP + N-acetylneuraminyl-2,3-beta-D-galactosyl-1,4-beta-D-glucosylceramide

Homo sapiens

Q9UNP4

-

658140

-

?

CMP-N-acetylneuraminate + lactosylceramide

CMP + N-acetylneuraminyl-2,3-beta-D-galactosyl-1,4-beta-D-glucosylceramide

Gallus gallus

-

synthesizes the first ganglioside of the gangliotetraose series, GM3

638776

-

CMP-N-acetylneuraminate + lactosylceramide

CMP + N-acetylneuraminyl-2,3-beta-D-galactosyl-1,4-beta-D-glucosylceramide

Mesocricetus auratus

-

involved in sequential addition of monosaccharides from sugar nucleotides to non-reducing end of oligosaccharide chains of glycosphingolipids

638785

-

CMP-N-acetylneuraminate + lactosylceramide

CMP + alpha-N-acetylneuraminyl-2,3-beta-D-galactosyl-1,4-beta-D-glucosyl-ceramide

Mus musculus

-

673509

-

?

CMP-N-acetylneuraminate + lactosylceramide

CMP + alpha-N-acetylneuraminyl-2,3-beta-D-galactosyl-1,4-beta-D-glucosyl-ceramide

Homo sapiens

-, Q9UNP4

-

672358

-

?

CMP-N-acetylneuraminate + lactosylceramide

CMP + alpha-N-acetylneuraminyl-2,3-beta-D-galactosyl-1,4-beta-D-glucosyl-ceramide

Homo sapiens

-

674638, 721691

-

?

CMP-N-acetylneuraminate + lactosylceramide

CMP + alpha-N-acetylneuraminyl-2,3-beta-D-galactosyl-1,4-beta-D-glucosyl-ceramide

Mus musculus

-

ganglioside GM3 is involved in neuronal cell death

673509

-

?

CMP-N-acetylneuraminate + lactosylceramide

CMP + alpha-N-acetylneuraminyl-2,3-beta-D-galactosyl-1,4-beta-D-glucosyl-ceramide

Homo sapiens

-

metabolic flux and expression levels of enzymes involved in the sialic acid biosynthetic pathway, overview

674638

-

?

CMP-N-acetylneuraminate + lactosylceramide

?

Homo sapiens

-

100% relative activity

705676

-

?

CMP-Neu5Ac + lactosylceramide

?

Homo sapiens

-

caveolin-1 is a GM3-interacting protein in GM3 synthase overexpressing cells

703916

-

?

additional information

?

-

Mus musculus

-

mice with a disrupted gene encoding GM3 synthase have an enhanced phosphorylation of the skeletal muscle insulin receptor after ligand binding. They show heightened responses in glucose and insulin tolerance tests. The mutant mice are protected from high-fat diet induced insulin resistance

660348

-

additional information

?

-

Homo sapiens

-

the cAMP responsive element binding protein binding site of the hST3Gal V promoter plays a critical role in transcriptional regulation of the hST3Gal V gene during HL-60 cell differentiation

658723

-

additional information

?

-

Homo sapiens

-

upregulation of GM3 synthase through protein kinase C/extracellular regulated kinases-dependent cAMP-responsive element binding protein activation results in the differentiation of HL-60 cells by inducing expression of CD11b

658853

-

additional information

?

-

Homo sapiens

-

overexpression of recombinant UDP-GlcNAc 2-epimerase/ManNAc 6-kinase, GNE, in HEK AD293 cells up-regulates GM3 synthase, while GNE down-regulation by RNA interference or exogenous GM3 and GD3 down-regulates the GM3 synthase

674638

-

additional information

?

-

Homo sapiens

-, Q9UNP4

substrate specificity of isozymes, no activity with GD3 and GM1a as acceptors, overview

672358

-

additional information

?

-

Homo sapiens

-

no acitvity with GM1 or asialofetuin as acceptor

705676

-

additional information

?

-

Homo sapiens

-

the -177 to -83 region, which contains a cAMP-responsive element at -143, functions as the valproic acid-inducible promoter by actively binding cAMP-responsive element binding protein. The cAMP-responsive element at -143 is crucial for the valproic acid-induced expression of hST3Gal V in SK-NBE(2)-C cells

701568

-

CMP-N-acetylneuraminate + lactosylceramide

CMP + N-acetylneuraminyl-2,3-beta-D-galactosyl-1,4-beta-D-glucosylceramide

Gallus gallus

-

synthesizes the first ganglioside of the gangliotetraose series, GM3

638776

-

CMP-N-acetylneuraminate + lactosylceramide

CMP + N-acetylneuraminyl-2,3-beta-D-galactosyl-1,4-beta-D-glucosylceramide

Mesocricetus auratus

-

involved in sequential addition of monosaccharides from sugar nucleotides to non-reducing end of oligosaccharide chains of glycosphingolipids

638785

-

CMP-N-acetylneuraminate + lactosylceramide

CMP + alpha-N-acetylneuraminyl-2,3-beta-D-galactosyl-1,4-beta-D-glucosyl-ceramide

Homo sapiens

-, Q9UNP4

-

672358

-

?

CMP-N-acetylneuraminate + lactosylceramide

CMP + alpha-N-acetylneuraminyl-2,3-beta-D-galactosyl-1,4-beta-D-glucosyl-ceramide

Mus musculus

-

ganglioside GM3 is involved in neuronal cell death

673509

-

?

CMP-N-acetylneuraminate + lactosylceramide

CMP + alpha-N-acetylneuraminyl-2,3-beta-D-galactosyl-1,4-beta-D-glucosyl-ceramide

Homo sapiens

-

metabolic flux and expression levels of enzymes involved in the sialic acid biosynthetic pathway, overview

674638

-

?

additional information

?

-

Mus musculus

-

mice with a disrupted gene encoding GM3 synthase have an enhanced phosphorylation of the skeletal muscle insulin receptor after ligand binding. They show heightened responses in glucose and insulin tolerance tests. The mutant mice are protected from high-fat diet induced insulin resistance

660348

-

additional information

?

-

Homo sapiens

-

the cAMP responsive element binding protein binding site of the hST3Gal V promoter plays a critical role in transcriptional regulation of the hST3Gal V gene during HL-60 cell differentiation

658723

-

additional information

?

-

Homo sapiens

-

upregulation of GM3 synthase through protein kinase C/extracellular regulated kinases-dependent cAMP-responsive element binding protein activation results in the differentiation of HL-60 cells by inducing expression of CD11b

658853

-

additional information

?

-

Homo sapiens

-

overexpression of recombinant UDP-GlcNAc 2-epimerase/ManNAc 6-kinase, GNE, in HEK AD293 cells up-regulates GM3 synthase, while GNE down-regulation by RNA interference or exogenous GM3 and GD3 down-regulates the GM3 synthase

674638

-

No entries in this field

Ca2+

Mesocricetus auratus

-

activation, slightly less efficient than Mn2+

Mg2+

-

Mg2+

-

activation, slightly less efficient than Mn2+

638785

Mg2+

Homo sapiens

-, Q9UNP4

;

Mn2+

-

Mn2+

-

10 mM, approx. 2fold activation, required for full activity

638783

Mn2+

Mesocricetus auratus

-

15 mM, required for maximal activity

638785

additional information

Rattus norvegicus

-

no metal ion requirement

637736

additional information

Homo sapiens

-

no metal ion requirement

637739

(4-Amidinophenyl)-methanesulfonyl fluoride

Rattus norvegicus

-

serine protease, 0.02 mM, 48% inhibition of purified sialyltransferase-1

638781

2D-image

alpha2-Macroglobulin

Rattus norvegicus

-

1 unit, 67% inhibition of purified sialyltransferase-1, no inhibition of sialyltransferase-1 activity in microsomes

638781

-

Aprotinin

Rattus norvegicus

-

serine protease inhibitor, 0.0003 mM, 74% inhibition of purified sialyltransferase-1

638781

2D-image

asialofetuin

Mesocricetus auratus

-

weak

638785

-

Ba2+

Rattus norvegicus

-

weak

637736

2D-image

Ca2+

Rattus norvegicus

-

weak

637736

2D-image

Cd2+

Rattus norvegicus

-

strong

CDP

-

CMP

-

CMP

-

CMPdialdehyde

-

kinetics

Co2+

-

CTP

-

Cu2+

-

strong

637736

2D-image

dithiothreitol

Rattus norvegicus

-

0.1 mM, 89% inhibition of purified sialyltransferase-1

638781

2D-image

DL-threo-phenyl-2-hexadecanoylamino-3-pyrrolidino-1-propanol

Homo sapiens

-

inhibitor of ganglioside synthesis, depletion of GM3 synthesis in cultured monocyte-derived macrophages, delays acquisition of CD206 antigen, prevents loss of CD163 antigen and enhances anti-inflammatory cytokine (CCL18) secretion

705676

2D-image

E-64

Rattus norvegicus

-

thiol protease inhibitor, 0.0028 mM, 71% inhibition of purified sialyltransferase-1, activation of sialytransferase-1 activity in microsomes

638781

2D-image

EDTA

Rattus norvegicus

-

0.1 mM, 71% inhibition of purified sialyltransferase-1

638781

2D-image

Ep-475

Rattus norvegicus

-

thiol protease inhibitor, 0.0028 mM, 75% inhibition of purified sialyltransferase-1; thiol protease inhibitor, 0.0028 mM, 84% inhibition of purified sialyltransferase-1

ganglioside GM1a

-

ganglioside GM3

-

0.5 mM and 1 mM, 43% and 60% inhibition respectively

638785

2D-image

Globotetraosylceramide

Mesocricetus auratus

-

weak

638785

2D-image

Go6976

Homo sapiens

-

protein kinase C inhibitor, treatment of phorbol 12-myristate 13-acetate induced cells reduces expression of GM3 synthase

Ionic detergents

-

-

L-1-chloro-3-[4-tosylamido]-4-phenyl-2-butanone

Rattus norvegicus

-

chymotrypsin and thiol protease inhibitor, 0.284 mM, 67% inhibition of purified sialyltransferase-1

638781

2D-image

L-1-Chloro-3-[4-tosylamido]-7-amino-2-heptanone-HCl

Rattus norvegicus

-

trypsin and thiol protease inhibitor, 0.135 mM, 73% inhibition of purified sialyltransferase-1

638781

2D-image

Leupeptin

Rattus norvegicus

-

serine and thiol protease inhibitor, 0.001 mM, 72% inhibition of purified sialyltransferase-1, no inhibition of sialyltransferase-1 activity in microsomes

638781

2D-image

Mn2+

Rattus norvegicus

-

weak

637736

2D-image

N3-

Rattus norvegicus

-

1 mM, 87% inhibition of purified sialyltransferase-1

638781

2D-image

Ni2+

Rattus norvegicus

-

strong

637736

2D-image

pepstatin A

Rattus norvegicus

-

0.001 mM, 72% inhibition of purified sialyltransferase-1, activation of sialyltransferase-1 activity in microsomes

638781

2D-image

Tris buffer

Homo sapiens

-

weak

637739

2D-image

Triton CF-54

Rattus norvegicus

-

at higher concentrations

637737

2D-image

U0126

Homo sapiens

-

extracellular signal-regulated kinase inhibitor, treatment of phorbol 12-myristate 13-acetate induced cells reduces expression of GM3 synthase

UDP

-

UDP-N-acetylgalactosamine

Gallus gallus

-

weak

638776

2D-image

UDPdialdehyde

Gallus gallus

-

kinetics

638776

2D-image

UDPgalactose

Gallus gallus

-

weak

638776

2D-image

Zn2+

Rattus norvegicus

-

weak

637736

2D-image

Zn2+

Rattus norvegicus

-

10 mM, 90% inhibition

638783

2D-image

Monoclonal antibody M12GC7

Rattus norvegicus

-

638780

-

additional information

Gallus gallus

-

not inhibited by N-ethylmaleimide

636879

-

additional information

Rattus norvegicus

-

not inhibited by EDTA; not inhibited by Fe2+, Mg2+, IAA, 2-mercaptoethanol

637736

-

additional information

Homo sapiens

-

not inhibited by EDTA

637739

-

additional information

Rattus norvegicus

-

inhibition in vivo i.e. stable microsomes not as dramatic as in vitro

638781

-

additional information

Mesocricetus auratus

-

glucosylceramide or globotriaosylceramide

638785

-

additional information

Homo sapiens

-

overexpression of recombinant UDP-GlcNAc 2-epimerase/ManNAc 6-kinase, GNE, in HEK AD293 cells up-regulates GM3 synthase, while GNE down-regulation by RNA interference or exogenous GM3 and GD3 down-regulates the GM3 synthase

674638

-

additional information

Homo sapiens

-

motility of low GM3 synthase expressing cells is reduced in the presence of a Src kinase inhibitor

703916

-

2-mercaptoethanol

Rattus norvegicus

-

0.18 mM, 392% activation of purified sialyltransferase-1, 1120% activation of sialytransferase-1 activity in microsomes

beta-octylglucoside

-

cardiolipin

-

activation

637739

2D-image

Cutscum

Homo sapiens

-

activation, most potent activator; can be replaced by Triton CF-54, Triton X-100 or Triton CF-54/Tween 80, ratio 2:1, activation efficiency in descending order

637739

-

Cutscum

Mesocricetus auratus

-

638785

-

Myrj 59

Rattus norvegicus

-

activation, most potent activator, can be replaced by sodium deoxycholate, Triton CF-54, Tween 20, Triton CF-54/Tween 80, ratio 2:1, Triton X-100 or Tween 80, activation efficiency in descending order

638784

2D-image

Nonidet P-40

Homo sapiens

-

slight activation

637739

2D-image

Triton CF-54

Rattus norvegicus

-

inhibits at higher concentrations

Triton CF-54

-

Triton CF-54

-

0.15%, required for full activity

Triton CF-54

-

Triton CF-54

-

activation, most potent activator

638785

2D-image

Triton CF-54/Tween 80

Homo sapiens

-

ratio 2:1, activation, about 50% as efficient as Cutscum

637739

-

Triton CF-54/Tween 80

Rattus norvegicus

-

less than 50% as efficient as Myrj 59

638784

-

Triton CF-54/Tween 80

Mesocricetus auratus

-

638785

-

Triton X-100

Homo sapiens

-

activation

637739

2D-image

Triton X-100

Rattus norvegicus

-

activation

637736, 638782, 638784

2D-image

Triton X-100

Mesocricetus auratus

-

activation

638785

2D-image

Lauryldimethylamine oxide

Rattus norvegicus

-

i.e. Ammonyx LO, nonionic/cationic detergent, 12-15fold higher activation than by Triton CF-54, Triton X-100 or beta-octylglucoside

638782

2D-image

additional information

Mesocricetus auratus

-

not activated by Tween 20 or 80

638785

-

additional information

Mus musculus

-

GM3 is up-regulated in glutamate-induced neuronal cell death

673509

-

additional information

Homo sapiens

-

overexpression of recombinant UDP-GlcNAc 2-epimerase/ManNAc 6-kinase, GNE, in HEK AD293 cells up-regulates GM3 synthase, while GNE down-regulation by RNA interference or exogenous GM3 and GD3 down-regulates the GM3 synthase

674638

-

additional information

Homo sapiens

-

the enzyme is expressed during the induction of megakaryocytic differentiation in human leukemia K562 cells by phorbol 12-myristate 13-acetate depending upon the PKC/ERK/CREB pathway, overview

675938

-

0.00026

-

CMP-N-acetylneuraminate

Rattus norvegicus

-

638780

2D-image

0.16

-

CMP-N-acetylneuraminate

Mesocricetus auratus

-

638785

2D-image

0.18

-

CMP-N-acetylneuraminate

Homo sapiens

-

705676

2D-image

0.19

-

CMP-N-acetylneuraminate

Homo sapiens

-

636877

2D-image

0.21

-

CMP-N-acetylneuraminate

Rattus norvegicus

-

638783

2D-image

1.5

-

CMP-N-acetylneuraminate

Rattus norvegicus

-

637736

2D-image

1.5

-

CMP-N-acetylneuraminate

Homo sapiens

-

637739

2D-image

0.00011

-

lactosylceramide

Rattus norvegicus

-

638780

2D-image

0.035

-

lactosylceramide

Homo sapiens

-

637739

2D-image

0.068

-

lactosylceramide

Rattus norvegicus

-

637737

2D-image

0.075

-

lactosylceramide

Homo sapiens

-

636877

2D-image

0.08

-

lactosylceramide

Rattus norvegicus

-

637736, 638783

2D-image

0.0815

-

lactosylceramide

Gallus gallus

-

638776

2D-image

0.101

-

lactosylceramide

Homo sapiens

-

705676

2D-image

0.11

-

lactosylceramide

Mesocricetus auratus

-

638785

2D-image

No entries in this field

0.0853

-

CMP-dialdehyde

Gallus gallus

-

at 0.1 mM

638776

2D-image

0.104

-

CMP-dialdehyde

Gallus gallus

-

at 0.2 mM

638776

2D-image

0.219

-

UDP-dialdehyde

Gallus gallus

-

at 0.2 mM

638776

2D-image

No entries in this field

0.000000167

-

Homo sapiens

-

homogenate

705676

0.0000027

-

Oreochromis mossambicus

-

sialyltransferase-1 activity in membrane fraction from brain

636888

0.0000032

-

Homo sapiens

-

liver

658140

0.0000045

-

Homo sapiens

-

637739

0.0000064

-

Rattus norvegicus

-

sialyltransferase-1 activity in membrane fraction from brain

636888

0.00002255

-

Homo sapiens

-

PMA-stimulated HL-60 cell

658140

0.0006048

-

Homo sapiens

-

3600fold purified enzyme

705676

0.0055

-

Rattus norvegicus

-

638780

0.0094

-

Rattus norvegicus

-

638783

additional information

-

Homo sapiens

-

analysis of cell surface and total levels of GM3 in wild-type and transfected cells

674638

additional information

-

Mus musculus

-

activity of ks-M3-SAT-I is about 3fold higher than that of either ks-M1-SAT-I or ks-M2-SAT-I

705616

5.7

-

Rattus norvegicus

-

637736

6

-

Homo sapiens

-

637739

6.2

-

Homo sapiens

-

636877

6.4

-

Homo sapiens

-, Q9UNP4

assay at; assay at

672358

6.5

-

Rattus norvegicus

-

638780, 638783

6.5

-

Mesocricetus auratus

-

broad optimum in cacodylate-HCl buffer

638785

additional information

-

Rattus norvegicus

-

pI: 5.7-6.2

638780

additional information

-

Mesocricetus auratus

-

no effects of buffer on activity

638785

4.5

8

Mesocricetus auratus

-

638785

6.1

7.6

Rattus norvegicus

-

detectable activity

638780

37

-

Homo sapiens

-

636877

37

-

Homo sapiens

-, Q9UNP4

assay at; assay at

672358

No entries in this field

4T1 cell

Mus musculus

-

expression of GM3S mRNA is greater in the highly metastatic 4T1 tumor cells than in the nonmetastatic 67NR cells

702982

aorta

Homo sapiens

-

level of GM3 synthase in membrane-fractions isolated from the atherosclerotic intima are higher compared to those in non-dieased arterial tissue

657703

aorta

Homo sapiens

-

atherosclerotic aortic intima

658140

aorta

Homo sapiens

-

atherosclerotic and normal aorta

-

-

-

in atherosclerosis, excessive GM3 is synthesized in atherosclerotic lesions by macrophages and dendritic cells directly within the arterial walls in blood arteries additionally to the GM3 synthesis in blood plasma, the fatty acid compositions of GM3 from blood plasma low density lipoprotein and from atherosclerotic lesions differ; level of GM3 synthase in membrane-fractions isolated from the atherosclerotic intima are higher compared to those in non-dieased arterial tissue

657703

blood

Homo sapiens

-

in atherosclerosis, excessive GM3 is synthesized in atherosclerotic lesions by macrophages and dendritic cells directly within the arterial walls in blood vessels additionally to the GM3 synthesis in blood plasma, the fatty acid compositions of GM3 from blood plasma low density lipoprotein and from atherosclerotic lesions differ

657703

brain

Oreochromis mossambicus

-

-

-

636888, 638783, 638784, 638791

-

-, Q9UNP4

fetal, isozymes type 1-4

-

-

high levels of SAT-Ia-1 and SAT-Ia-2, whereas very low levels of SAT-Ib-1 and SAT-Ib-2

705616

brain

Mus musculus

-

three SAT-I isoforms, high transcript level of SAT-Ia and SAT-Ib transcript

705616

brain

Rattus norvegicus Sprague-Dawley

-

breast cancer cell

Mus musculus

-

702982

cell suspension culture

-

-

; excessive GM3 is synthesized in atherosclerotic lesions by macrophages and dendritic cells directly within the arterial walls

657703

fibroblast

Mesocricetus auratus

-

contact-inhibited cell-line NIL-8

638785

heart

Mus musculus

-

high transcript level of SAT-Ia and SAT-Ib transcript

-

-

strain R, elevated enzyme level if cultured in butyrate containing media

-

-

phorbol myristate actetate-stimulated

658140

HL-60 cell

Homo sapiens

-

the cAMP responsive element binding protein binding site of the hST3Gal V promoter plays a critical role in transcriptional regulation of the hST3Gal V gene during HL-60 cell differentiation

658723

HL-60 cell

Homo sapiens

-

upregulation of GM3 synthase through protein kinase C/extracellular regulated kinases-dependent cAMP-responsive element binding protein activation results in the differentiation of HL-60 cells by inducing expression of CD11b

658853

HL-60 cell

Homo sapiens

-, Q9UNP4

type 2 isozyme

-

-

GM3 synthase expression analysis, the enzyme is expressed during the induction of megakaryocytic differentiation in human leukemia K562 cells by phorbol 12-myristate 13-acetate depending upon the PKC/ERK/CREB pathway, overview

675938

K-562 cell

Homo sapiens

-

treatment of phorbol 12-myristate 13-acetate induced cells with protein kinase C inhibitor Go6976 or extracellular signal-regulated kinase inhibitor U0126 reduces expression of GM3 synthase. Activation of extracellular signal-regulated kinase and cAMP response element binding protein is prevented by pretreatment with Go6976 and U0126

-

-

637736, 637737, 637742, 638779, 638780, 638781, 638782, 638788, 638792

-

-

high levels of SAT-Ia-1 and SAT-Ia-2, whereas very low levels of SAT-Ib-1 and SAT-Ib-2

705616

lymphocyte

Homo sapiens

-

from peripheral blood mononuclear cells, enzyme level is stimulated by growth in the presence of phytohemagglutinin

-

-

; excessive GM3 is synthesized in atherosclerotic lesions by macrophages and dendritic cells directly within the arterial walls

657703

macrophage

Homo sapiens

-

differentiated monocyte-derived macrophages

-

-

murine neuroblastoma x rat dorsal root ganglion F-11A cells

489583

neuron

Rattus norvegicus

-

brain neuron

-

-

high levels of SAT-Ia-1 and SAT-Ia-2, whereas very low levels of SAT-Ib-1 and SAT-Ib-2

705616

P-19 cell

Mus musculus

-

embryonic carcinoma cell line P19 differentiates into neurons and astrocytes on cell aggregation after treatment with retinoic acid

638793

peripheral blood mononuclear cell

-

-, Q9UNP4

type 5 isozyme

672358

retinal pigment epithelial cell

-

-

high levels of SAT-Ia-1 and SAT-Ia-2, whereas very low levels of SAT-Ib-1 and SAT-Ib-2

705616

spleen

Homo sapiens

-

high levels of SAT-Ia-1 and SAT-Ia-2, whereas very low levels of SAT-Ib-1 and SAT-Ib-2

705616

testis

Homo sapiens

-

high levels of SAT-Ia-1 and SAT-Ia-2, whereas very low levels of SAT-Ib-1 and SAT-Ib-2

705616

testis

Mus musculus

-

high transcript level of SAT-Ia and SAT-Ib transcript

705616

thymus

Mus musculus

-

the GM3 synthase gene is expressed at relatively higher levels in the dexamethasone-administered mice than in the control mice

658868

thymus

Homo sapiens

-

high levels of SAT-Ia-1 and SAT-Ia-2, whereas very low levels of SAT-Ib-1 and SAT-Ib-2

705616

thyroid

Homo sapiens

-

high levels of SAT-Ia-1 and SAT-Ia-2, whereas very low levels of SAT-Ib-1 and SAT-Ib-2

-

additional information

Homo sapiens

-

immunohistochemic analysis of tissue distribution of GM3 synthase

657703

additional information

Homo sapiens

-

SK-N-BE(2)-C neuroblastoma cell

701568

additional information

Mus musculus

-

67NR cell

702982

additional information

Homo sapiens

-

in cervix, high levels of SAT-Ia-1 and SAT-Ia-2, whereas very low levels of SAT-Ib-1 and SAT-Ib-2

705616

additional information

Mus musculus

-

in B16 melanoma cells SAT-Ia is present

705616

endoplasmic reticulum

Mus musculus

-

broadley distributed in endoplasmic reticulum

5783

489583

endoplasmic reticulum

Mus musculus

-

exclusively M1-SAT-I, specific multiple arginines arranged in an R-based motif, RRXXXXR necessary for endoplasmic reticulum targeting, in the cytoplasmic tail of M1-SAT-I

-

broadley distributed in Golgi

-

distribution

-

luminal side

-

637736, 637737, 638780, 638781, 638788, 638792

Golgi apparatus

Mus musculus

-

M2- and M3-SAT-I. Isoform M2-SAT-I is rapidly degraded in the lysosomes, whereas isoform M3-SAT-I is retained in the Golgi apparatus

-

139

657703

membrane

Oreochromis mossambicus

-

-

636888, 637736, 638784

membrane

Homo sapiens

-

level of GM3 synthase in membrane-fractions isolated from the atherosclerotic intima are higher compared to those in non-dieased arterial tissue

16020

657703

membrane

Rattus norvegicus Sprague-Dawley

-

-

-

-

-, Q9UNP4

membrane and lumen; membrane and lumen

-

672358

additional information

Mus musculus

-

M1-SAT-I, M2-SAT-I, and M3-SAT-I possess distinct lengths in their NH2-terminal cytoplasmic tails

-

705616

No entries in this field

?

Rattus norvegicus

-

x * 60000, SDS-PAGE

638780, 638782

?

Rattus norvegicus

-

x * 76000, SDS-PAGE

638783

?

Homo sapiens

-

x * 60000, SDS-PAGE

658140

additional information

Homo sapiens

-, Q9UNP4

the isozyme transcripts differ in length and the proteins in molecular weight; the isozyme transcripts differ in length and the proteins in molecular weight

672358

glycoprotein

Homo sapiens

-, Q9UNP4

;

672358

glycoprotein

Rattus norvegicus

-

N- and O-linked carbohydrate side chains, containing sialic acids in alpha-2,3 and 2,6-linkage, galactose and galactosamine, branched N-glycans containing mannose

638780

No entries in this field

56

-

Rattus norvegicus

-

t1/2: 60 s

637737

100

-

Homo sapiens

-

5 min, inactivation

637739

lauryldimethylamine oxide solubilizes and stabilizes solubilized enzyme during purification and storage

Rattus norvegicus

-

638782

PMSF, leupeptin or pepstatin A stabilize during purification

Rattus norvegicus

-

638781

No entries in this field

-80°C, 25 mM sodium cacodylate, pH 6.5, 15% w/v lauryldimethylamine oxide, 6-12 months, no loss of activity

Rattus norvegicus

-

638782

by immunoaffinity chromatography, to apparent homogeneity, 3600fold, with a yield of 19%

-

-

-

CHO cells transfected with hSAT-Ia-1 or -Ia-2 including the 5'-UTR

Homo sapiens

-

705616

cloning from HL-60 cells, DNA and amino acid sequence determination and analysis, genetic organization of isozymes, mRNA variants differ in the 5'-untranslated region, in vitro transcription and translation using the rabbit reticulocyte lysate system, expression in murine MG1361 cells, a mammary adenocarcinoma cell line; cloning from placenta, DNA and amino acid sequence determination and analysis, genetic organization of isozymes, mRNA variants differ in the 5'-untranslated region, in vitro transcription and translation using the rabbit reticulocyte lysate system, expression in murine MG1361 cells, a mammary adenocarcinoma cell line; type 1-4 isozymes, DNA and amino acid sequence determination and analysis, genetic organization of isozymes, mRNA variants differ in the 5'-untranslated region, in vitro transcription and translation using the rabbit reticulocyte lysate system, expression in murine MG1361 cells, a mammary adenocarcinoma cell line

Homo sapiens

-, Q9UNP4

672358

from HL-60 cell DNA, expression of His6-tagged fragment comprising residues 191-245

Homo sapiens

-

657703

SAT-1 cDNA cloned into plasmid expression vector pRc/CMV under control of CMV promoter, overexpression in A2780 ovarian tumor cells

Homo sapiens

-

703916

CHO cells transiently transfected with plasmids encoding SAT-Ia or SAT-Ib, which contains the 5'-untranslated region of each variant. CHO cells stably transfected with ks-M1-SAT-I, ks-M2-SAT-I, or ks-M3-SAT-I, in which GCCACC (ks) is inserted before isoforms M1, M2, or M3. Stable expression in CHO cells of a fusion protein for the NH2-terminus of M1-, M2-, or M3-SAT-I containing the transmembrane region and EGFP (M1-SAT-I(N)-EGFP, M2-SAT-I(N)-EGFP, and M3-SAT-I(N)-EGFP or each M1-SAT-I(N)-EGFP mutant)

Mus musculus

-

705616

expression as fusion proteins with red fluorescent protein in murine neuroblastoma F-11A cells

Mus musculus

-

489583

functional expression in HT22 cells, GM3 levels are increased in immortalized mouse hippocampal HT22 cells after exposure to glutamate, GM3 not only mediates the effect of glutamate on the oxidative death of HT22 cells but also acts, in itself, as a modulator of in vivo neuronal cell death, overview

Mus musculus

-

673509

three pairs of oligonucleotides, corresponding to different regions of murine GM3S encoding gene, annealed and inserted into BglII/HindIII double digested pMEHMpuro, yielding the vectors pMEHM-siGM3S1, pMEHM-siGM3S2, and pMEHMsiGM3S3. Full-length GM3S encoding gene amplified from the total RNA of 4T1 cells and inserted into XhoI/EcoRI double digested pMSCVpuro, resulting in the GM3S expression vector pMSCV-GM3S. Overexpressed in non-metastatic 67NR cells

Mus musculus

-

702982

valproic acid induces hST3Gal V mRNA expression in SK-N-BE(2)-C cells. A cAMP-responsive element at -143 is essential for transcriptional activation of hST3Gal V in valproic acid-induced SK-N-BE(2)-C cells. Subsequent cAMP-responsive element binding protein binding to this cAMP-responsive element mediates valproic acid-dependent upregulation of hST3Gal V gene expression

Homo sapiens

-

701568

SAT-1 mRNA level and GM3 synthase activity in vitro are markedly upregulated in 3 SAT-1-transfected clones with respect to wild-type and mock-transfected A2780 cells

Homo sapiens

-

703916

GM3 synthase mRNA levels are significantly higher in differentiated monocyte-derived macrophages compared to monocytes and in atherosclerotic aorta compared to normal aorta

Homo sapiens

-

705676

GM3 synthase mRNA is significantly increased (mediated by specificity protein 1) in transforming growth factor-beta1-induced HLE B-3 cells

Homo sapiens

-

721545

the activity of GM3 synthase in blood mononuclear cells from atherosclerotic patients is 4.6fold higher than those from healthy subjects

Homo sapiens

-

721691

the transcriptional activity of the enzyme is induced 2.4fold by 1 mM valproic acid in ARPE-19 cells

Homo sapiens

-

721922

expression of GM3S in the highly metastatic 4T1 cell line silenced by RNA interference. Expression of the GM3S siRNAs siGM3S2 and siGM3S3, but not that of siGM3S1, drastically reduces expression of GM3S mRNA in 4T1 cells

Mus musculus

-

702982

expression level of M3-SAT-I decreases remarkably in cells carrying the mSAT-Ib m1 in which the nucleotide sequence from position -6 to -1 at the M2 site is switched for GCCACC

Mus musculus

-

705616

enzyme mRNA levels are significantly lower (64%) in mouse muscles of a model of hereditary inclusion body myopathy harboring the M712T mutation of UDP-N-acetylglucosamine 2-epimerase/N-acetylmannosamine kinase compared to control mice

Mus musculus

-

723516

overexpressed in 67NR cells

Mus musculus

-

702982

DELTA12-21

Mus musculus

-

M1-SAT-I(N)-EGFP mutant, is localized in the Golgi apparatus

705616

DELTA12-26

Mus musculus

-

M1-SAT-I(N)-EGFP mutant, is localized in the Golgi apparatus

705616

DELTA28-55

Mus musculus

-

M1-SAT-I(N)-EGFP mutant, changes its localization from the endoplasmic reticulum to the Golgi apparatus

705616

M28/56A

Mus musculus

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ks-M1-SAT-I mutant, subcellular localization and stability similar to those of the wild-type. Activity is similar to that of ks-M1-SAT-I. Amounts of gangliosides in the ks-M1-SAT-I M28/56A mutant-transfected cells are greatly reduced compared with those of cells transfected with the other isoforms or mutants

705616

M29A

Mus musculus

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ks-M1-SAT-I mutant, activity is decreased to ca. 50%, relative to the activity of ks-M2-SAT-I

705616

R11S

Mus musculus

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M1-SAT-I(N)-EGFP mutant, is localized in the endoplasmic reticulum

705616

R11S/R12S

Mus musculus

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M1-SAT-I(N)-EGFP mutant, results in a shift of localization from the endoplasmic reticulum to the Golgi apparatus

705616

R11S/R17S

Mus musculus

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M1-SAT-I(N)-EGFP mutant, shifts its localization to the Golgi apparatus

705616

R12S

Mus musculus

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M1-SAT-I(N)-EGFP mutant, is localized in the endoplasmic reticulum

705616

R12S/R17S

Mus musculus

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M1-SAT-I(N)-EGFP mutant, shifts its localization to the Golgi apparatus

705616

R17S

Mus musculus

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M1-SAT-I(N)-EGFP mutant, stays in the endoplasmic reticulum

705616

additional information

Homo sapiens

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overexpression of recombinant UDP-GlcNAc 2-epimerase/ManNAc 6-kinase, GNE, in HEK AD293 cells up-regulates GM3 synthase, while GNE down-regulation by RNA interference or exogenous GM3 and GD3 down-regulates the GM3 synthase

674638

No entries in this field

medicine

Mus musculus

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GM3 synthase may be of value as a therapeutic target in breast cancer

702982

medicine

Mus musculus

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the enzyme is a marker of hereditary inclusion body myopathy

723516