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Information on EC 2.4.99.20 - 2'-phospho-ADP-ribosyl cyclase/2'-phospho-cyclic-ADP-ribose transferase and Organism(s) Mus musculus and UniProt Accession P56528

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IUBMB Comments
This multiunctional enzyme catalyses both the removal of nicotinamide from NADP+, forming 2'-phospho-cyclic ADP-ribose, and the addition of nicotinate to the cyclic product, forming NAADP+, a calcium messenger that can mobilize intracellular Ca2+ stores and activate Ca2+ influx to regulate a wide range of physiological processes. In addition, the enzyme also catalyses EC 3.2.2.6, ADP-ribosyl cyclase/cyclic ADP-ribose hydrolase.
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This record set is specific for:
Mus musculus
UNIPROT: P56528
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The taxonomic range for the selected organisms is: Mus musculus
The enzyme appears in selected viruses and cellular organisms
Synonyms
adp-ribosyl cyclase 1, more
SYNONYM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
BST1
-
-
diphosphopyridine nucleosidase
-
ambiguous
PATHWAY SOURCE
PATHWAYS
SYSTEMATIC NAME
IUBMB Comments
NADP+:nicotinate ADP-ribosyltransferase
This multiunctional enzyme catalyses both the removal of nicotinamide from NADP+, forming 2'-phospho-cyclic ADP-ribose, and the addition of nicotinate to the cyclic product, forming NAADP+, a calcium messenger that can mobilize intracellular Ca2+ stores and activate Ca2+ influx to regulate a wide range of physiological processes. In addition, the enzyme also catalyses EC 3.2.2.6, ADP-ribosyl cyclase/cyclic ADP-ribose hydrolase.
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
2'-phospho-cyclic ADP-ribose + nicotinate
nicotinate-adenine dinucleotide phosphate
show the reaction diagram
-
-
-
-
?
NADP+ + nicotinate
nicotinate-adenine dinucleotide phosphate + nicotinamide
show the reaction diagram
NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
NADP+ + nicotinate
nicotinate-adenine dinucleotide phosphate + nicotinamide
show the reaction diagram
-
this multiunctional enzyme catalyses both the removal of nicotinamide from NADP+, forming 2'-phospho-cyclic ADP-ribose, and the addition of nicotinate to the cyclic product, forming nicotinic acid-adenine dinucleotide phosphate, a calcium messenger that can mobilize intracellular Ca2+ stores and activate Ca2+ influx to regulate a wide range of physiological processes
-
-
?
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
0.00000013
-
37°C, pH 7.2, microsome from CD38(+/+) wild-type mouse brain
0.0000002
-
37°C, pH 7.2, mitochondria from CD38(+/+) wild-type mouse brain
0.0000016
-
37°C, pH 7.2, plasma membrane from CD38(+/+) wild-type mouse brain
0.4
-
37°C, pH 7.2, nucleus from CD38(+/+) wild-type mouse brain
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
7.2
-
assay at
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
37
-
assay at
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
-
0.13 nM/min*mg
-
Manually annotated by BRENDA team
-
0.2 nM/min*mg
Manually annotated by BRENDA team
-
0.4 nM/min*mg
Manually annotated by BRENDA team
-
1.6 nM/min*mg
Manually annotated by BRENDA team
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
physiological function
malfunction
-
generation of CD38(-/-) knockout mice of determine the role of this enzyme
physiological function
UNIPROT
ENTRY NAME
ORGANISM
NO. OF AA
NO. OF TRANSM. HELICES
MOLECULAR WEIGHT[Da]
SOURCE
SEQUENCE
LOCALIZATION PREDICTION?
CD38_MOUSE
304
1
34408
Swiss-Prot
other Location (Reliability: 5)
EXPRESSION
ORGANISM
UNIPROT
LITERATURE
CD38 is intensely increased in cisplatin-treated mice
-
REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Chini, E.N.; Chini, C.C.; Kato, I.; Takasawa, S.; Okamoto, H.
CD38 is the major enzyme responsible for synthesis of nicotinic acid-adenine dinucleotide phosphate in mammalian tissues
Biochem. J.
362
125-130
2002
Mus musculus, Rattus norvegicus (Q64244)
Manually annotated by BRENDA team
Schmid, F.; Bruhn, S.; Weber, K.; Mittruecker, H.W.; Guse, A.H.
CD38: a NAADP degrading enzyme
FEBS Lett.
585
3544-3548
2011
Homo sapiens (P28907), Mus musculus (P56528)
Manually annotated by BRENDA team
Rah, S.Y.; Mushtaq, M.; Nam, T.S.; Kim, S.H.; Kim, U.H.
Generation of cyclic ADP-ribose and nicotinic acid adenine dinucleotide phosphate by CD38 for Ca2+ signaling in interleukin-8-treated lymphokine-activated killer cells
J. Biol. Chem.
285
21877-21887
2010
Mus musculus (P56528)
Manually annotated by BRENDA team
El-Hamoly, T.; El-Sharawy, D.; El Refaye, M.; Abd El-Rahman, S.
L-Thyroxine modifies nephrotoxicity by regulating the apoptotic pathway The possible role of CD38/ADP-ribosyl cyclase-mediated calcium mobilization
PLoS ONE
12
e0184157
2017
Mus musculus
Manually annotated by BRENDA team