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Information on EC 2.4.99.20 - 2'-phospho-ADP-ribosyl cyclase/2'-phospho-cyclic-ADP-ribose transferase and Organism(s) Aplysia californica and UniProt Accession P29241

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IUBMB Comments
This multiunctional enzyme catalyses both the removal of nicotinamide from NADP+, forming 2'-phospho-cyclic ADP-ribose, and the addition of nicotinate to the cyclic product, forming NAADP+, a calcium messenger that can mobilize intracellular Ca2+ stores and activate Ca2+ influx to regulate a wide range of physiological processes. In addition, the enzyme also catalyses EC 3.2.2.6, ADP-ribosyl cyclase/cyclic ADP-ribose hydrolase.
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This record set is specific for:
Aplysia californica
UNIPROT: P29241
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The taxonomic range for the selected organisms is: Aplysia californica
The enzyme appears in selected viruses and cellular organisms
Synonyms
adp-ribosyl cyclase 1, more
SYNONYM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
2'-phospho-cyclic-ADP-ribose transferase
-
ADP-ribosyl cyclase 1
-
PATHWAY SOURCE
PATHWAYS
SYSTEMATIC NAME
IUBMB Comments
NADP+:nicotinate ADP-ribosyltransferase
This multiunctional enzyme catalyses both the removal of nicotinamide from NADP+, forming 2'-phospho-cyclic ADP-ribose, and the addition of nicotinate to the cyclic product, forming NAADP+, a calcium messenger that can mobilize intracellular Ca2+ stores and activate Ca2+ influx to regulate a wide range of physiological processes. In addition, the enzyme also catalyses EC 3.2.2.6, ADP-ribosyl cyclase/cyclic ADP-ribose hydrolase.
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
2'-phospho-cyclic ADP-ribose + nicotinate
nicotinate-adenine dinucleotide phosphate
show the reaction diagram
NADP+
2'-phospho-cyclic ADP-ribose + nicotinamide
show the reaction diagram
-
-
-
?
NGDP+
2'-phospho-cyclic GDP-ribose + nicotinamide
show the reaction diagram
enzyme cyclizes nicotinamide guanine dinucleotide to produce a fluorescent product, cyclic GDP-ribose, which has a site of cyclization different from cADPR
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-
?
additional information
?
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KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
5
nicotinate
pH 5, 22°C
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
UNIPROT
ENTRY NAME
ORGANISM
NO. OF AA
NO. OF TRANSM. HELICES
MOLECULAR WEIGHT[Da]
SOURCE
SEQUENCE
LOCALIZATION PREDICTION?
NADA_APLCA
282
0
31899
Swiss-Prot
Secretory Pathway (Reliability: 1)
CRYSTALLIZATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
enzyme is a dimer
REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Lee, H.C.; Graeff, R.M.; Walseth, T.F.
ADP-ribosyl cyclase and CD38. Multi-functional enzymes in Ca+2 signaling
Adv. Exp. Med. Biol.
419
411-419
1997
Homo sapiens (P28907), Aplysia californica (P29241)
Manually annotated by BRENDA team
Moreschi, I.; Bruzzone, S.; Melone, L.; De Flora, A.; Zocchi, E.
NAADP+ synthesis from cADPRP and nicotinic acid by ADP-ribosyl cyclases
Biochem. Biophys. Res. Commun.
345
573-580
2006
Axinella polypoides, Homo sapiens (P28907), Aplysia californica (P29241)
Manually annotated by BRENDA team
Aarhus, R.; Graeff, R.M.; Dickey, D.M.; Walseth, T.F.; Lee, H.C.
ADP-ribosyl cyclase and CD38 catalyze the synthesis of a calcium-mobilizing metabolite from NADP+
J. Biol. Chem.
270
30327-30333
1995
Homo sapiens (P28907), Aplysia californica (P29241)
Manually annotated by BRENDA team