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Information on EC 2.4.99.18 - dolichyl-diphosphooligosaccharide-protein glycotransferase
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2.4.99.18 dolichyl-diphosphooligosaccharide-protein glycotransferaseIUBMB Comments
Occurs in eukaryotes that form a glycoprotein by the transfer of a glucosyl-mannosyl-glucosamine polysaccharide to the side-chain of an L-asparagine residue in the sequence -Asn-Xaa-Ser- or -Asn-Xaa-Thr- (Xaa not Pro) in nascent polypeptide chains. The basic oligosaccharide is the tetradecasaccharide Glc3Man9GlcNAc2 (for diagram {polysacc/Dol14}). However, smaller oligosaccharides derived from it and oligosaccharides with additional monosaccharide units attached may be involved. See ref for a review of N-glycoproteins in eukaryotes. Man3GlcNAc2 seems to be common for all of the oligosaccharides involved with the terminal N-acetylglucosamine linked to the protein L-asparagine. Occurs on the cytosolic face of the endoplasmic reticulum. The dolichol involved normally has 14-21 isoprenoid units with two trans double-bonds at the omega end, and the rest of the double-bonds in cis form.
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Word Map
- 2.4.99.18
-
n-glycosylation
-
n-linked
- glycans
-
lipid-linked
-
sequons
-
asparagine-linked
- jejuni
- campylobacter
- dolichol
-
ribophorins
- otase
-
cotranslational
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bloc
-
dolichol-linked
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translocon
- glc3man9glcnac2
- heptasaccharide
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s-layer
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underglycosylation
-
preassembled
-
mannosyltransferases
-
glycoengineering
-
hypoglycosylation
-
hetero-oligomeric
-
single-subunit
-
translocon-associated
- man9glcnac2
- lari
- drug development
- biotechnology
- medicine
- n-glycoproteins
-
glycoproteomics
- pilins
The expected taxonomic range for this enzyme is: Eukaryota, Archaea, Bacteria
Reaction Schemes
Synonyms
oligosaccharyltransferase, oligosaccharyl transferase, oligosaccharyltransferase complex, ost3p, wbp1p, ost6p, oligosaccharide transferase, swp1p, tbstt3a, tbstt3b, 
more
topSYNONYM 
ORGANISM
UNIPROT
COMMENTARY 
LITERATURE
asparagine N-glycosyltransferase
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-
-
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dolichyldiphosphooligosaccharide-protein oligosaccharyltransferase
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-
-
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glycosyltransferase, dolichyldiphosphooligosaccharide-protein
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-
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glycosyltransferase, dolichylpyrophosphodiacetylchitobiose-protein
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-
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oligomannosyltransferase
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-
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oligosaccharide transferase
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-
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oligosaccharyltransferase, dolichyldiphosphoryloligosaccharide-protein
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-
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-
OST
-
-
-
-
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
hexosyl group transfer
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-
-
-
SYSTEMATIC NAME
IUBMB Comments
dolichyl-diphosphooligosaccharide:protein-L-asparagine N-beta-D-oligopolysaccharidotransferase
Occurs in eukaryotes that form a glycoprotein by the transfer of a glucosyl-mannosyl-glucosamine polysaccharide to the side-chain of an L-asparagine residue in the sequence -Asn-Xaa-Ser- or -Asn-Xaa-Thr- (Xaa not Pro) in nascent polypeptide chains. The basic oligosaccharide is the tetradecasaccharide Glc3Man9GlcNAc2 (for diagram {polysacc/Dol14}). However, smaller oligosaccharides derived from it and oligosaccharides with additional monosaccharide units attached may be involved. See ref [2] for a review of N-glycoproteins in eukaryotes. Man3GlcNAc2 seems to be common for all of the oligosaccharides involved with the terminal N-acetylglucosamine linked to the protein L-asparagine. Occurs on the cytosolic face of the endoplasmic reticulum. The dolichol involved normally has 14-21 isoprenoid units with two trans double-bonds at the omega end, and the rest of the double-bonds in cis form.
CAS REGISTRY NUMBER
COMMENTARY
75302-32-8
-
SUBSTRATE
PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
(Substrate)
LITERATURE
(Substrate)
(Substrate)
COMMENTARY
(Product)
(Product)
LITERATURE
(Product)
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
additional information
?
-
it may be that the lumenal domain of Ost1p is involved in funneling the newly synthesized polypeptides into the active site on Stt3p for the glycosylation oligosaccharyl transferase reaction
-
-
?
additional information
?
-
it may be that the lumenal domain of Ost1p is involved in funneling the newly synthesized polypeptides into the active site on Stt3p for the glycosylation oligosaccharyl transferase reaction
-
-
?
additional information
?
-
it may be that the lumenal domain of Ost1p is involved in funneling the newly synthesized polypeptides into the active site on Stt3p for the glycosylation oligosaccharyl transferase reaction
-
-
?
additional information
?
-
it may be that the lumenal domain of Ost1p is involved in funneling the newly synthesized polypeptides into the active site on Stt3p for the glycosylation oligosaccharyl transferase reaction
-
-
?
additional information
?
-
it may be that the lumenal domain of Ost1p is involved in funneling the newly synthesized polypeptides into the active site on Stt3p for the glycosylation oligosaccharyl transferase reaction
-
-
?
additional information
?
-
it may be that the lumenal domain of Ost1p is involved in funneling the newly synthesized polypeptides into the active site on Stt3p for the glycosylation oligosaccharyl transferase reaction
-
-
?
additional information
?
-
it may be that the lumenal domain of Ost1p is involved in funneling the newly synthesized polypeptides into the active site on Stt3p for the glycosylation oligosaccharyl transferase reaction
-
-
?
additional information
?
-
-
it may be that the lumenal domain of Ost1p is involved in funneling the newly synthesized polypeptides into the active site on Stt3p for the glycosylation oligosaccharyl transferase reaction
-
-
?
NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
(Substrate)
LITERATURE
(Substrate)
(Substrate)
COMMENTARY
(Product)
(Product)
LITERATURE
(Product)
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
additional information
?
-
it may be that the lumenal domain of Ost1p is involved in funneling the newly synthesized polypeptides into the active site on Stt3p for the glycosylation oligosaccharyl transferase reaction
-
-
?
additional information
?
-
it may be that the lumenal domain of Ost1p is involved in funneling the newly synthesized polypeptides into the active site on Stt3p for the glycosylation oligosaccharyl transferase reaction
-
-
?
additional information
?
-
it may be that the lumenal domain of Ost1p is involved in funneling the newly synthesized polypeptides into the active site on Stt3p for the glycosylation oligosaccharyl transferase reaction
-
-
?
additional information
?
-
it may be that the lumenal domain of Ost1p is involved in funneling the newly synthesized polypeptides into the active site on Stt3p for the glycosylation oligosaccharyl transferase reaction
-
-
?
additional information
?
-
it may be that the lumenal domain of Ost1p is involved in funneling the newly synthesized polypeptides into the active site on Stt3p for the glycosylation oligosaccharyl transferase reaction
-
-
?
additional information
?
-
it may be that the lumenal domain of Ost1p is involved in funneling the newly synthesized polypeptides into the active site on Stt3p for the glycosylation oligosaccharyl transferase reaction
-
-
?
additional information
?
-
it may be that the lumenal domain of Ost1p is involved in funneling the newly synthesized polypeptides into the active site on Stt3p for the glycosylation oligosaccharyl transferase reaction
-
-
?
additional information
?
-
-
it may be that the lumenal domain of Ost1p is involved in funneling the newly synthesized polypeptides into the active site on Stt3p for the glycosylation oligosaccharyl transferase reaction
-
-
?
ORGANISM
COMMENTARY
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY
GeneOntology No.
LITERATURE
SOURCE
oligosaccharyl transferase consists of nine different subunits, all of which contain one or more predicted transmembrane segments. The functional domain of Ost1p is its membrane-anchored lumenal domain
UNIPROT
ENTRY NAME
ORGANISM
NO. OF AA
NO. OF TRANSM. HELICES
MOLECULAR WEIGHT[Da]
SOURCE
SEQUENCE
LOCALIZATION PREDICTION
The reliability class of the localization prediction ranges from 1 (very reliable) to 5 (not reliable).
The reliability class of the localization prediction ranges from 1 (very reliable) to 5 (not reliable). OST1_YEAST
Saccharomyces cerevisiae (strain ATCC 204508 / S288c)
476
0
54072
Swiss-Prot
-
PDB
SCOP
CATH
UNIPROT
ORGANISM
SUBUNIT
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
additional information
additional information
oligosaccharyl transferase consists of nine different subunits, all of which contain one or more predicted transmembrane segments. In yeast, five of these proteins are encoded by essential genes, Swp1p, Wbp1p, Ost2p, Ost1p and Stt3p
additional information
oligosaccharyl transferase consists of nine different subunits, all of which contain one or more predicted transmembrane segments. In yeast, five of these proteins are encoded by essential genes, Swp1p, Wbp1p, Ost2p, Ost1p and Stt3p
additional information
oligosaccharyl transferase consists of nine different subunits, all of which contain one or more predicted transmembrane segments. In yeast, five of these proteins are encoded by essential genes, Swp1p, Wbp1p, Ost2p, Ost1p and Stt3p
additional information
oligosaccharyl transferase consists of nine different subunits, all of which contain one or more predicted transmembrane segments. In yeast, five of these proteins are encoded by essential genes, Swp1p, Wbp1p, Ost2p, Ost1p and Stt3p
additional information
oligosaccharyl transferase consists of nine different subunits, all of which contain one or more predicted transmembrane segments. In yeast, five of these proteins are encoded by essential genes, Swp1p, Wbp1p, Ost2p, Ost1p and Stt3p
additional information
oligosaccharyl transferase consists of nine different subunits, all of which contain one or more predicted transmembrane segments. In yeast, five of these proteins are encoded by essential genes, Swp1p, Wbp1p, Ost2p, Ost1p and Stt3p
additional information
oligosaccharyl transferase consists of nine different subunits, all of which contain one or more predicted transmembrane segments. In yeast, five of these proteins are encoded by essential genes, Swp1p, Wbp1p, Ost2p, Ost1p and Stt3p
additional information
-
oligosaccharyl transferase consists of nine different subunits, all of which contain one or more predicted transmembrane segments. In yeast, five of these proteins are encoded by essential genes, Swp1p, Wbp1p, Ost2p, Ost1p and Stt3p
CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Li, G.; Yan, Q.; Nita-Lazar, A.; Haltiwanger, R.S.; Lennarz, W.J.
Studies on the N-glycosylation of the subunits of oligosaccharyl transferase in Saccharomyces cerevisiae
J. Biol. Chem.
280
1864-1871
2005
Saccharomyces cerevisiae (P33767), Saccharomyces cerevisiae (P39007), Saccharomyces cerevisiae (P41543), Saccharomyces cerevisiae (P46964), Saccharomyces cerevisiae (P48439), Saccharomyces cerevisiae (Q02795), Saccharomyces cerevisiae (Q03723), Saccharomyces cerevisiae (Q92316), Saccharomyces cerevisiae (Q99380), Saccharomyces cerevisiae
Lennarz, W.J.
Studies on oligosaccharyl transferase in yeast
Acta Biochim. Pol.
54
673-677
2007
Saccharomyces cerevisiae (P33767), Saccharomyces cerevisiae (P41543), Saccharomyces cerevisiae (P46964), Saccharomyces cerevisiae (P48439), Saccharomyces cerevisiae (Q02795), Saccharomyces cerevisiae (Q92316), Saccharomyces cerevisiae (Q99380), Saccharomyces cerevisiae
EXTERNAL LINKS (specific for EC-Number 2.4.99.18)
Transporter Classification Database (TCDB): 9.B.142.3.17, 9.B.142.3.14, 9.B.142.3.4, 9.B.142.3.5, 9.B.142.3.3
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