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Information on EC 2.4.99.17 - S-adenosylmethionine:tRNA ribosyltransferase-isomerase and Organism(s) Bacillus subtilis and UniProt Accession O32054

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IUBMB Comments
The reaction is a combined transfer and isomerization of the ribose moiety of S-adenosyl-L-methionine to the modified guanosine base in the wobble position in tRNAs specific for Tyr, His, Asp or Asn. It is part of the queuosine biosynthesis pathway.
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This record set is specific for:
Bacillus subtilis
UNIPROT: O32054
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Word Map
The taxonomic range for the selected organisms is: Bacillus subtilis
The expected taxonomic range for this enzyme is: Bacteria, Archaea
Reaction Schemes
+
7-aminomethyl-7-carbaguanosine34 in tRNA
=
+
+
epoxyqueuosine34 in tRNA
Synonyms
s-adenosylmethionine:trna ribosyltransferase-isomerase, quea enzyme, more
SYNONYM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
S-adenosylmethionine:tRNA ribosyltransferase-isomerase
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QueA enzyme
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-
-
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queuosine biosynthesis protein QueA
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-
-
-
PATHWAY SOURCE
PATHWAYS
-
-, -
SYSTEMATIC NAME
IUBMB Comments
S-adenosyl-L-methionine:7-aminomethyl-7-deazaguanosine ribosyltransferase (ribosyl isomerizing; L-methionine, adenine releasing)
The reaction is a combined transfer and isomerization of the ribose moiety of S-adenosyl-L-methionine to the modified guanosine base in the wobble position in tRNAs specific for Tyr, His, Asp or Asn. It is part of the queuosine biosynthesis pathway.
CAS REGISTRY NUMBER
COMMENTARY hide
149719-25-5
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SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
S-adenosyl-L-methionine + 7-aminomethyl-7-carbaguanosine34 in tRNA
L-methionine + adenine + epoxyqueuosine34 in tRNA
show the reaction diagram
-
-
-
?
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
Mg2+
millimolar inhibitor
Mn2+
millimolar inhibitor
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
-
UniProt
Manually annotated by BRENDA team
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
metabolism
the enzyme is involved in the biosynthesis of the hypermodified tRNA nucleoside queuosine
CRYSTALLIZATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
sitting drop vapor diffusion method, using 100 mM Tris/HCl (pH 8.0), 200 mM (NH4)2SO4, 3 mM dithiothreitol, and 25-29% (w/v) PEG 4000
PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Grimm, C.; Ficner, R.; Sgraja, T.; Haebel, P.; Klebe, G.; Reuter, K.
Crystal structure of Bacillus subtilis S-adenosylmethionine:tRNA ribosyltransferase-isomerase
Biochem. Biophys. Res. Commun.
351
695-701
2006
Bacillus subtilis (O32054), Bacillus subtilis
Manually annotated by BRENDA team