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Information on EC 2.4.2.9 - uracil phosphoribosyltransferase and Organism(s) Arabidopsis thaliana and UniProt Accession Q9FKS0

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EC Tree
     2 Transferases
         2.4 Glycosyltransferases
             2.4.2 Pentosyltransferases
                2.4.2.9 uracil phosphoribosyltransferase
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This record set is specific for:
Arabidopsis thaliana
UNIPROT: Q9FKS0 not found.
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Word Map
The taxonomic range for the selected organisms is: Arabidopsis thaliana
The expected taxonomic range for this enzyme is: Bacteria, Eukaryota, Archaea
Synonyms
uracil phosphoribosyltransferase, uprt, uprtase, uracil phosphoribosyl transferase, cd-uprt, ump pyrophosphorylase, cytosine deaminase/uracil phosphoribosyltransferase, mtuprt, cytosine deaminase-uracil phosphoribosyltransferase, uridine monophosphate pyrophosphorylase, more
SYNONYM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
uridine kinase-like protein (uracil phosphoribosyltransferase)
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uridine kinase/uracil phosphoribosyltransferase 1
the enzyme contains a uridine domain and the C-terminal region consists of a UPRT domain
phosphoribosyltransferase, uracil
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-
-
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UMP pyrophosphorylase
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-
-
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UMP:pyrophosphate phosphoribosyltransferase
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-
-
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UPRTase
-
-
-
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uridine 5'-phosphate pyrophosphorylase
-
-
-
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uridine monophosphate pyrophosphorylase
-
-
-
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uridylate pyrophosphorylase
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-
-
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uridylic pyrophosphorylase
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-
-
-
additional information
cf. EC 2.7.1.48
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
pentosyl group transfer
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-
-
-
PATHWAY SOURCE
PATHWAYS
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-
SYSTEMATIC NAME
IUBMB Comments
UMP:diphosphate phospho-alpha-D-ribosyltransferase
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CAS REGISTRY NUMBER
COMMENTARY hide
9030-24-4
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SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
5-fluorouracil + 5-phospho-alpha-D-ribose 1-diphosphate
5-fluoro-UMP + diphosphate
show the reaction diagram
uracil + 5-phospho-alpha-D-ribose 1-diphosphate
UMP + diphosphate
show the reaction diagram
-
-
-
?
uridine + ATP
UMP + ADP
show the reaction diagram
the bifunctional enzyme UK/UPRT also performs the uridine kinase reaction, EC 2.7.1.48
-
-
?
5-fluorouracil + 5-phospho-alpha-D-ribose 1-diphosphate
5-fluoro-UMP + diphosphate
show the reaction diagram
-
-
-
-
?
uracil + 5-phospho-alpha-D-ribose 1-diphosphate
UMP + diphosphate
show the reaction diagram
NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
5-fluorouracil + 5-phospho-alpha-D-ribose 1-diphosphate
5-fluoro-UMP + diphosphate
show the reaction diagram
recombinant bifunctional enzyme UK/UPRT
-
-
?
uracil + 5-phospho-alpha-D-ribose 1-diphosphate
UMP + diphosphate
show the reaction diagram
-
-
-
?
uridine + ATP
UMP + ADP
show the reaction diagram
the bifunctional enzyme UK/UPRT also performs the uridine kinase reaction, EC 2.7.1.48
-
-
?
uracil + 5-phospho-alpha-D-ribose 1-diphosphate
UMP + diphosphate
show the reaction diagram
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
additional information
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
forming granular precipitates
Manually annotated by BRENDA team
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
metabolism
the enzyme is involved in the metabolism of uracil and related compounds in plants catalyzing the uracil salvage, which is of major importance for plant development, overview
physiological function
UPRTs, on-essential, energy-saving enzymes, are involved in the salvage of pyrimidines by catalyzing the formation of uridine monophosphate from uracil and phosphoribosylpyrophosphate. Uracil salvage is of major importance for plant development, especially early development
UNIPROT
ENTRY NAME
ORGANISM
NO. OF AA
NO. OF TRANSM. HELICES
MOLECULAR WEIGHT[Da]
SOURCE
SEQUENCE
LOCALIZATION PREDICTION?
UKL1_ARATH
486
0
54430
Swiss-Prot
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MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
53000
x * 53000, recombinant UK/UPRT1, SDS-PAGE
SUBUNIT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
?
x * 53000, recombinant UK/UPRT1, SDS-PAGE
additional information
PROTEIN VARIANTS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
additional information
construction of T-DNA insertion enzyme mutants, inability to salvage uracil caused a lightdependent dramatic pale-green to albino phenotype, dwarfism and the inability to produce viable progeny in loss-of-function mutants, plastid biogenesis and starch accumulation are affected in all tissues, with the exception of stomata, phenotypes, overview. A graduation in the tolerance to 5-fluorouracil is observed among the upp(+/) heterozygous plants. Functional complementation of the enzyme-deficient Escherichia coli mutant strain BM604, overview. No significant decrease in UPRT activity is measured in the mutants disrupted at uracil kinase-like proteins UKL loci
CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
DNA and amino acid sequence determination and analysis of the natural chimeric gene UK/UPRT1 encoding uridine kinase, UK, and uracil phosphoribosyltransferase, UPRT, expressionin Escherichia coli, transient expression as C-terminally GFP-tagged enzyme in Arabidopsis thaliana mesophyll protoplasts, 5-FU sensitivity of the Escherichia coli upp mutants is influenced by the expression of AtUK/UPRT1, as well as the 5-FU and 5-FD sensitivity of Escherichia coli upp-udk mutants, overview
expressed in Escherichia coli strain JM109
expressed in HeLa cells
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gene UPP, DNA and amino acid sequence determination and analysis, gene structure, sequence comparison, and phylogenetic analysis, Comparison to uracil kinase-like proteins possessing UPRT domains. The single nuclear gene encoding UPP targeted to plastids is responsible for almost all UPRT activity in Arabidopsis thaliana
APPLICATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
medicine
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the enzyme augments the cancer treatment efficacy of 5-fluorouracil in vitro
REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Islam, M.R.; Kim, H.; Kang, S.W.; Kim, J.S.; Jeong, Y.M.; Hwang, H.J.; Lee, S.Y.; Woo, J.C.; Kim, S.G.
Functional characterization of a gene encoding a dual domain for uridine kinase and uracil phosphoribosyltransferase in Arabidopsis thaliana
Plant Mol. Biol.
63
465-477
2007
Arabidopsis thaliana (Q9FKS0), Arabidopsis thaliana
Manually annotated by BRENDA team
Mainguet, S.E.; Gakiere, B.; Majira, A.; Pelletier, S.; Bringel, F.; Guerard, F.; Caboche, M.; Berthome, R.; Renou, J.P.
Uracil salvage is necessary for early Arabidopsis development
Plant J.
60
280-291
2009
Arabidopsis thaliana (Q9M336)
Manually annotated by BRENDA team
Narayanan, S.; Sanpui, P.; Sahoo, L.; Ghosh, S.S.
Unravelling the potential of a new uracil phosphoribosyltransferase (UPRT) from Arabidopsis thaliana in sensitizing HeLa cells towards 5-fluorouracil
Int. J. Biol. Macromol.
91
310-316
2016
Arabidopsis thaliana
Manually annotated by BRENDA team