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EC Tree
IUBMB Comments The enzyme, found in animals and insects, is involved in the biosynthesis of the alpha-D-xylosyl-(1->3)-alpha-D-xylosyl-(1->3)-beta-D-glucosyl trisaccharide on epidermal growth factor-like (EGF-like) domains. When present on Notch proteins, the trisaccharide functions as a modulator of the signalling activity of this protein.
The enzyme appears in viruses and cellular organisms
Reaction Schemes
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[protein with EGF-like domain]-3-O-[alpha-D-xylosyl-(1->3)-beta-D-glucosyl]-L-serine
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[protein with EGF-like domain]-3-O-[alpha-D-xylosyl-(1->3)-alpha-D-xylosyl-(1->3)-beta-D-glucosyl]-L-serine
Synonyms
c3orf21, xxylt1, xyloside xylosyltransferase 1,
more
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UDP-xylose:alpha-xyloside alpha1,3-xylosyltransferase
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xyloside alpha-1,3-xylosyltransferase
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xyloside alpha1-3 xylosyltransferase
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xyloside xylosyltransferase 1
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XXYLT1
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UDP-alpha-D-xylose + [protein with EGF-like domain]-3-O-[alpha-D-xylosyl-(1->3)-beta-D-glucosyl]-L-serine = UDP + [protein with EGF-like domain]-3-O-[alpha-D-xylosyl-(1->3)-alpha-D-xylosyl-(1->3)-beta-D-glucosyl]-L-serine
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UDP-alpha-D-xylose:[EGF-like domain protein]-3-O-[alpha-D-xylosyl-(1->3)-beta-D-glucosyl]-L-serine 3-alpha-D-xylosyltransferase (configuration-retaining)
The enzyme, found in animals and insects, is involved in the biosynthesis of the alpha-D-xylosyl-(1->3)-alpha-D-xylosyl-(1->3)-beta-D-glucosyl trisaccharide on epidermal growth factor-like (EGF-like) domains. When present on Notch proteins, the trisaccharide functions as a modulator of the signalling activity of this protein.
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UDP-alpha-D-xylose + Xyl-Glc-O-[protein with EGF-like domain 16]
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UDP-alpha-D-xylose + Xylalpha(1->3)Xylalpha(1->3)Glcbeta-O-(CH2)3NHCOC7H15
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UDP-alpha-D-xylose + [protein with EGF-like domain]-3-O-[alpha-D-xylosyl-(1->3)-beta-D-glucosyl]-L-serine
UDP + [protein with EGF-like domain]-3-O-[alpha-D-xylosyl-(1->3)-alpha-D-xylosyl-(1->3)-beta-D-glucosyl]-L-serine
UDP-D-xylose + N-pyridin-2-yl-3-O-alpha-D-xylopyranosyl-beta-D-glucopyranosylamine
UDP + alpha-D-xylopyranosyl-(1-3)-alpha-D-xylopyranosyl-(1-3)-N-pyridin-2-yl-beta-D-glucopyranosylamine
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the structure of the transfer product isolated is confirmed by Smith degradation, mass spectrometry, and a- and y9-xylosidase digestions
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additional information
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UDP-alpha-D-xylose + [protein with EGF-like domain]-3-O-[alpha-D-xylosyl-(1->3)-beta-D-glucosyl]-L-serine
UDP + [protein with EGF-like domain]-3-O-[alpha-D-xylosyl-(1->3)-alpha-D-xylosyl-(1->3)-beta-D-glucosyl]-L-serine
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UDP-alpha-D-xylose + [protein with EGF-like domain]-3-O-[alpha-D-xylosyl-(1->3)-beta-D-glucosyl]-L-serine
UDP + [protein with EGF-like domain]-3-O-[alpha-D-xylosyl-(1->3)-alpha-D-xylosyl-(1->3)-beta-D-glucosyl]-L-serine
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UDP-alpha-D-xylose + [protein with EGF-like domain]-3-O-[alpha-D-xylosyl-(1->3)-beta-D-glucosyl]-L-serine
UDP + [protein with EGF-like domain]-3-O-[alpha-D-xylosyl-(1->3)-alpha-D-xylosyl-(1->3)-beta-D-glucosyl]-L-serine
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additional information
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the enzyme participates in the biosynthesis of the Glc-O-Ser-type sugar chains
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additional information
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the enzyme cannot act on a synthetic acceptor containing an alpha-linked xylose alone and has no activity with 4-nitrophenyl-alpha-D-xylose and Xyl-O-[protein with EGF-like domain]
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additional information
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the enzyme cannot act on a synthetic acceptor containing an alpha-linked xylose alone and has no activity with 4-nitrophenyl-alpha-D-xylose and Xyl-O-[protein with EGF-like domain]
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UDP-alpha-D-xylose + [protein with EGF-like domain]-3-O-[alpha-D-xylosyl-(1->3)-beta-D-glucosyl]-L-serine
UDP + [protein with EGF-like domain]-3-O-[alpha-D-xylosyl-(1->3)-alpha-D-xylosyl-(1->3)-beta-D-glucosyl]-L-serine
additional information
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the enzyme participates in the biosynthesis of the Glc-O-Ser-type sugar chains
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UDP-alpha-D-xylose + [protein with EGF-like domain]-3-O-[alpha-D-xylosyl-(1->3)-beta-D-glucosyl]-L-serine
UDP + [protein with EGF-like domain]-3-O-[alpha-D-xylosyl-(1->3)-alpha-D-xylosyl-(1->3)-beta-D-glucosyl]-L-serine
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UDP-alpha-D-xylose + [protein with EGF-like domain]-3-O-[alpha-D-xylosyl-(1->3)-beta-D-glucosyl]-L-serine
UDP + [protein with EGF-like domain]-3-O-[alpha-D-xylosyl-(1->3)-alpha-D-xylosyl-(1->3)-beta-D-glucosyl]-L-serine
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UDP-alpha-D-xylose + [protein with EGF-like domain]-3-O-[alpha-D-xylosyl-(1->3)-beta-D-glucosyl]-L-serine
UDP + [protein with EGF-like domain]-3-O-[alpha-D-xylosyl-(1->3)-alpha-D-xylosyl-(1->3)-beta-D-glucosyl]-L-serine
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Mg2+
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restores activity after inhibition by EDTA
Mn2+
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restores activity after inhibition by EDTA
Mn2+
10 mM used in assay conditions
Mn2+
required for activity
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EDTA
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activity is restored by addition of Mn2+ and Mg2
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Adenocarcinoma of Lung
Association between C3orf21, TP63 polymorphisms and environment and NSCLC in never-smoking Chinese population.
Adenocarcinoma of Lung
C3orf21 ablation promotes the proliferation of lung adenocarcinoma, and its mutation at the rs2131877 locus may serve as a susceptibility marker.
Adenocarcinoma of Lung
XXYLT1 methylation contributes to the occurrence of lung adenocarcinoma: Methylation and lung adenocarcinoma.
Carcinoma, Non-Small-Cell Lung
Beta-Elemene Reduces the Malignancy of Non-Small Cell Lung Cancer by Enhancing C3orf21 Expression.
Lung Neoplasms
Beta-Elemene Reduces the Malignancy of Non-Small Cell Lung Cancer by Enhancing C3orf21 Expression.
Lung Neoplasms
C3orf21 ablation promotes the proliferation of lung adenocarcinoma, and its mutation at the rs2131877 locus may serve as a susceptibility marker.
Neoplasms
Beta-Elemene Reduces the Malignancy of Non-Small Cell Lung Cancer by Enhancing C3orf21 Expression.
Neoplasms
C3orf21 ablation promotes the proliferation of lung adenocarcinoma, and its mutation at the rs2131877 locus may serve as a susceptibility marker.
Neoplasms
XXYLT1 methylation contributes to the occurrence of lung adenocarcinoma: Methylation and lung adenocarcinoma.
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N-pyridin-2-yl-3-O-alpha-D-xylopyranosyl-beta-D-glucopyranosylamine
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pH 7.2, 37°C
0.28
UDP-D-xylose
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pH 7.2, 37°C
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UniProt
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UniProt
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metabolism
the enzyme transfers xylose from the donor UDP-alpha-D-xylose to Xyl-Glc disaccharide-modified EGF repeats from Notch1, Notch2 or human factor IX
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XXLT1_HUMAN
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1
43807
Swiss-Prot
Mitochondrion (Reliability: 3 )
XXLT1_MOUSE
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43839
Swiss-Prot
Mitochondrion (Reliability: 3 )
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in complex with acceptor Xyl-Glc-[EGF], hanging drop vapor diffusion method, using 0.2 M Li2SO4, 0.1 M Bis-Tris, pH 6.5, and 21% (w/v) PEG3350
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IgG-Sepharose-6 column chromatography and Superdex S200 gel filtration
Ni-NTA column chromatography and Superdex S200 gel filtration
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expressed in HEK-293T cells
expressed in Sf9 insect cells
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Minamida, S.; Aoki, K.; Natsuka, S.; Omichi, K.; Fukase, K.; Kusumoto, S.; Hase S.
Detection of UDP-D-xylose: alpha-D-xyloside alpha 1-->3xylosyltransferase activity in human hepatoma cell line HepG2
J. Biochem.
120
1002-1006
1996
Homo sapiens
brenda
Sethi, M.; Buettner, F.; Ashikov, A.; Krylov, V.; Takeuchi, H.; Nifantiev, N.; Haltiwanger, R.; Gerardy-Schahn, R.; Bakker, H.
Molecular cloning of a xylosyltransferase that transfers the second xylose to O-glucosylated epidermal growth factor repeats of Notch
J. Biol. Chem.
287
2739-2748
2012
Homo sapiens (Q8NBI6), Homo sapiens
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Yan, L.; Liu, Y.
The retaining mechanism of xylose transfer catalyzed by xyloside alpha-1,3-xylosyltransferase (XXYLT1) a quantum mechanics/molecular mechanics study
J. Chem. Inf. Model.
60
1585-1594
2020
Mus musculus (Q3U4G3)
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Yu, H.; Takeuchi, M.; LeBarron, J.; Kantharia, J.; London, E.; Bakker, H.; Haltiwanger, R.; Li, H.; Takeuchi, H.
Notch-modifying xylosyltransferase structures support an SNi-like retaining mechanism
Nat. Chem. Biol.
11
847-854
2015
Mus musculus (Q3U4G3), Mus musculus
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