Information on EC 2.4.2.6 - nucleoside deoxyribosyltransferase

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The expected taxonomic range for this enzyme is: Bacteria, Eukaryota

EC NUMBER
COMMENTARY
2.4.2.6
-
RECOMMENDED NAME
GeneOntology No.
nucleoside deoxyribosyltransferase
REACTION
REACTION DIAGRAM
COMMENTARY
ORGANISM
UNIPROT ACCESSION NO.
LITERATURE
2-deoxy-D-ribosyl-base1 + base2 = 2-deoxy-D-ribosyl-base2 + base1
show the reaction diagram
ping-pong bi bi mechanism
-
2-deoxy-D-ribosyl-base1 + base2 = 2-deoxy-D-ribosyl-base2 + base1
show the reaction diagram
Glu105, Asp-99 and Asn-172 are catalytic active site residues
-
2-deoxy-D-ribosyl-base1 + base2 = 2-deoxy-D-ribosyl-base2 + base1
show the reaction diagram
Glu90 is a catalytic active site residue, ligand binding and active site structures, overview
-
2-deoxy-D-ribosyl-base1 + base2 = 2-deoxy-D-ribosyl-base2 + base1
show the reaction diagram
-
-
-
-
REACTION TYPE
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
pentosyl group transfer
-
-
-
-
pentosyl group transfer
-
nucleoside 2-deoxyribose transfer
PATHWAY
KEGG Link
MetaCyc Link
Pyrimidine metabolism
-
SYSTEMATIC NAME
IUBMB Comments
nucleoside:purine(pyrimidine) deoxy-D-ribosyltransferase
Base1 and base2 represent various purines and pyrimidines.
SYNONYMS
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
2'-deoxyribosyltransferase
A5VK58
-
deoxynucleoside 5-monophosphate N-glycosidase
-
-
deoxyribose transferase
-
-
-
-
deoxyribosyltransferase, nucleoside
-
-
-
-
DRTase I
-
strictly specific for transfer between purine bases
DRTase II
-
catalyzes the transfer of the deoxyribosyl moiety between purines or pyrimidines as well as from a purine to a pyrimidine
N-deoxyribosyltransferase
-
-
N-deoxyribosyltransferase II
-
-
N-deoxyribosyltransferase II
Lactobacillus fermentum CGMCC 1.2133
-
-
-
NdRT-II
-
-
-
-
NTD
Lactobacillus fermentum PAK9
Q6YNI5
-
-
nucleoside 2'-deoxyribosyltransferase
Q6YNI5
-
nucleoside 2'-deoxyribosyltransferase
Lactobacillus fermentum PAK9
Q6YNI5
-
-
nucleoside 2'-deoxyribosyltransferase
-
-
nucleoside 2'-deoxyribosyltransferase
Q9RSV5
-
nucleoside 2'-deoxyribosyltransferase type II
-
-
nucleoside 2'-deoxyribosyltransferase type II
Bacillus coagulans CECT 12
-
-
-
nucleoside 2'-deoxyribosyltransferase type II
-
-
nucleoside 2'-deoxyribosyltransferase type II
Bacillus psychrosaccharolyticus CECT 4074
-
-
-
nucleoside 2'-deoxyribosyltransferase type II
-
-
nucleoside 2'-deoxyribosyltransferase type II
Lactobacillus sp. CECT 4219
-
-
-
nucleoside 2'-deoxyribosyltransferase type II
-
-
nucleoside 2'-deoxyribosyltransferase type II
Psychrobacter immobilis CECT 4492
-
-
-
nucleoside 2-deoxyribosyltransferase
-
-
nucleoside deoxyribosyltransferase I
-
purine nucleoside:purine deoxyribosyltransferase:strictly specific for transfer between purine bases
nucleoside deoxyribosyltransferase II
-
purine(pyrimidine) nucleoside:purine(pyrimidine) deoxyribosyltransferase:catalyzes the transfer of the deoxyribosyl moiety between purines or pyrimidines as well as from a purine to a pyrimidine
nucleoside N-deoxyribosyltransferase
-
-
nucleoside trans-N-deoxyribosylase
-
-
-
-
purine(pyrimidine) nucleoside:purine(pyrimidine) deoxyribosyl transferase
-
-
-
-
trans-deoxyribosylase
-
-
-
-
trans-N-deoxyribosylase
-
-
-
-
trans-N-deoxyribosylase
-
-
trans-N-glycosidase
-
-
-
-
transdeoxyribosylase
-
-
-
-
CAS REGISTRY NUMBER
COMMENTARY
9026-86-2
-
ORGANISM
COMMENTARY
LITERATURE
SEQUENCE CODE
SEQUENCE DB
SOURCE
strain CECT 12
-
-
Manually annotated by BRENDA team
Bacillus coagulans CECT 12
strain CECT 12
-
-
Manually annotated by BRENDA team
Bacillus psychrosaccharolyticus CECT 4074
strain CECT 4074
-
-
Manually annotated by BRENDA team
strain PAK9
UniProt
Manually annotated by BRENDA team
Lactobacillus fermentum CGMCC 1.2133
-
-
-
Manually annotated by BRENDA team
Lactobacillus fermentum PAK9
strain PAK9
UniProt
Manually annotated by BRENDA team
nucleoside deoxyribosyltransferase-I and nucleoside deoxyribosyltransferase-I
-
-
Manually annotated by BRENDA team
strain CECT 4219
-
-
Manually annotated by BRENDA team
Lactobacillus sp. CECT 4219
strain CECT 4219
-
-
Manually annotated by BRENDA team
subsp. lactis JCM5808
-
-
Manually annotated by BRENDA team
strain CECT 4492
-
-
Manually annotated by BRENDA team
Psychrobacter immobilis CECT 4492
strain CECT 4492
-
-
Manually annotated by BRENDA team
gene Rc1
-
-
Manually annotated by BRENDA team
SUBSTRATE
PRODUCT                      
REACTION DIAGRAM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
(Substrate)
LITERATURE
(Substrate)
COMMENTARY
(Product)
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
2',3'-dideoxyadenosine + cytosine
2',3'-dideoxycytidine + adenine
show the reaction diagram
Q6YNI5
-
-
-
?
2',3'-dideoxyadenosine + thymine
2',3'-dideoxythymidine + adenine
show the reaction diagram
Q6YNI5
-
-
-
?
2',3'-dideoxycytidine + adenine
2',3'-dideoxyadenosine + cytosine
show the reaction diagram
-, Q6YNI5
-
-
-
?
2',3'-dideoxycytidine + adenine
2',3'-dideoxyadenosine + cytosine
show the reaction diagram
Q6YNI5
-
-
-
?
2',3'-dideoxycytidine + adenine
2',3'-dideoxyadenosine + cytosine
show the reaction diagram
Q9RSV5
-
-
-
?
2',3'-dideoxycytidine + adenine
2',3'-dideoxyadenosine + cytosine
show the reaction diagram
Lactobacillus fermentum PAK9
Q6YNI5
-
-
-
?
2',3'-dideoxycytidine + hypoxanthine
2',3'-dideoxyinosine + cytosine
show the reaction diagram
-, Q6YNI5
-
-
-
?
2',3'-dideoxycytidine + hypoxanthine
2',3'-dideoxyinosine + cytosine
show the reaction diagram
Q6YNI5
-
-
-
?
2',3'-dideoxycytidine + hypoxanthine
2',3'-dideoxyinosine + cytosine
show the reaction diagram
Q9RSV5
-
-
-
?
2',3'-dideoxycytidine + hypoxanthine
2',3'-dideoxyinosine + cytosine
show the reaction diagram
Lactobacillus fermentum PAK9
Q6YNI5
-
-
-
?
2',3'-dideoxycytidine + thymine
2',3'-dideoxythymidine + cytosine
show the reaction diagram
Q6YNI5
-
-
-
?
2',3'-dideoxyguanosine + thymine
2',3'-dideoxythymidine + guanine
show the reaction diagram
-, Q6YNI5
-
-
-
?
2',3'-dideoxyguanosine + thymine
2',3'-dideoxythymidine + guanine
show the reaction diagram
Q9RSV5
-
-
-
?
2',3'-dideoxyguanosine + thymine
2',3'-dideoxythymidine + guanine
show the reaction diagram
Lactobacillus fermentum PAK9
Q6YNI5
-
-
-
?
2',3'-dideoxyinosine + adenine
2',3'-dideoxyadenosine + hypoxanthine
show the reaction diagram
-, Q6YNI5
-
-
-
?
2',3'-dideoxyinosine + adenine
2',3'-dideoxyadenosine + hypoxanthine
show the reaction diagram
Q9RSV5
-
-
-
?
2',3'-dideoxyinosine + adenine
2',3'-dideoxyadenosine + hypoxanthine
show the reaction diagram
Lactobacillus fermentum PAK9
Q6YNI5
-
-
-
?
2',3'-dideoxythymidine + adenine
2',3'-dideoxyadenosine + thymine
show the reaction diagram
-, Q6YNI5
-
-
-
?
2',3'-dideoxythymidine + adenine
2',3'-dideoxyadenosine + thymine
show the reaction diagram
Q6YNI5
-
-
-
?
2',3'-dideoxythymidine + adenine
2',3'-dideoxyadenosine + thymine
show the reaction diagram
Q9RSV5
-
-
-
?
2',3'-dideoxythymidine + adenine
2',3'-dideoxyadenosine + thymine
show the reaction diagram
Lactobacillus fermentum PAK9
Q6YNI5
-
-
-
?
2',3'-dideoxyuridine + cytosine
2',3'-dideoxycytidine + uracil
show the reaction diagram
-, Q6YNI5
-
-
-
?
2',3'-dideoxyuridine + cytosine
2',3'-dideoxycytidine + uracil
show the reaction diagram
Q9RSV5
-
-
-
?
2',3'didehydro-2',3'-dideoxythymidine + adenine
2',3'didehydro-2',3'-dideoxyadenosine + thymine
show the reaction diagram
-, Q6YNI5
-
-
-
?
2',3'didehydro-2',3'-dideoxythymidine + adenine
2',3'didehydro-2',3'-dideoxyadenosine + thymine
show the reaction diagram
Q6YNI5
-
-
-
?
2',3'didehydro-2',3'-dideoxythymidine + adenine
2',3'didehydro-2',3'-dideoxyadenosine + thymine
show the reaction diagram
Q9RSV5
-
-
-
?
2'-deoxy-D-ribosyl-uracil + adenine
2'-deoxy-D-ribosyl-adenine + uracil
show the reaction diagram
Bacillus coagulans, Lactobacillus sp., Psychrobacter immobilis, Bacillus psychrosaccharolyticus, Lactobacillus sp. CECT 4219, Bacillus psychrosaccharolyticus CECT 4074, Psychrobacter immobilis CECT 4492, Bacillus coagulans CECT 12
-
-
-
-
r
2'-deoxyadenosine + 5-chlorouracil
2'-deoxy-D-ribosyl-5-chlorouracil + adenine
show the reaction diagram
-
-
-
-
r
2'-deoxyadenosine + 5-chlorouracil
2'-deoxy-D-ribosyl-5-chlorouracil + adenine
show the reaction diagram
A5VK58, -
-
-
-
?
2'-deoxyadenosine + 5-chlorouracil
2'-deoxy-D-ribosyl-5-chlorouracil + adenine
show the reaction diagram
-
-
-
-
?
2'-deoxyadenosine + 5-chlorouracil
2'-deoxy-D-ribosyl-5-chlorouracil + adenine
show the reaction diagram
Lactobacillus sp. CECT 4219, Bacillus psychrosaccharolyticus CECT 4074, Psychrobacter immobilis CECT 4492, Bacillus coagulans CECT 12
-
-
-
-
r
2'-deoxyadenosine + 5-fluorouracil
2'-deoxy-5-fluorouridine + adenine
show the reaction diagram
Bacillus coagulans, Lactobacillus sp., Psychrobacter immobilis, Bacillus psychrosaccharolyticus, Lactobacillus sp. CECT 4219, Bacillus psychrosaccharolyticus CECT 4074, Psychrobacter immobilis CECT 4492, Bacillus coagulans CECT 12
-
-
-
-
r
2'-deoxyadenosine + 5-fluorouracil
2'-deoxy-D-ribosyl-5-fluorouracil + adenine
show the reaction diagram
Q9R5V5
reaction is catalyzed by mutants F13R/D92S/N123S and F13Q/D92S/N123S
-
-
?
2'-deoxyadenosine + cytosine
2'-deoxycytidine + adenine
show the reaction diagram
Q6YNI5
-
-
-
?
2'-deoxyadenosine + cytosine
2'-deoxycytidine + adenine
show the reaction diagram
-
-
-
-
?
2'-deoxyadenosine + thymine
2'-deoxythymidine + adenine
show the reaction diagram
Q6YNI5
-
-
-
?
2'-deoxycytidine + 2,6-diaminopurine
2,6-diaminopurine 2-deoxy-D-ribose 5-phosphate + cytosine
show the reaction diagram
Q9R5V5
reaction is catalyzed by mutants F13R/D92S/N123S and F13Q/D92S/N123S
-
-
?
2'-deoxycytidine + 2,6-dichloropurine
2,6-dichloropurine 2-deoxy-D-ribose 5-phosphate + cytosine
show the reaction diagram
Q9R5V5
reaction is catalyzed by mutants F13R/D92S/N123S and F13Q/D92S/N123S
-
-
?
2'-deoxycytidine + 5-bromouracil
2'-deoxy-D-ribosyl-5-bromouracil + cytosine
show the reaction diagram
Q9R5V5
reaction is catalyzed by mutants F13R/D92S/N123S and F13Q/D92S/N123S
-
-
?
2'-deoxycytidine + 6-chloropurine
6-chloropurine 2-deoxy-D-ribose 5-phosphate + cytosine
show the reaction diagram
Q9R5V5
reaction is catalyzed by mutants F13R/D92S/N123S and F13Q/D92S/N123S
-
-
?
2'-deoxycytidine + adenine
2'-deoxyadenosine + cytosine
show the reaction diagram
-, Q6YNI5
-
-
-
?
2'-deoxycytidine + adenine
2'-deoxyadenosine + cytosine
show the reaction diagram
Q6YNI5
-
-
-
?
2'-deoxycytidine + adenine
2'-deoxyadenosine + cytosine
show the reaction diagram
Q9RSV5
-
-
-
?
2'-deoxycytidine + adenine
2'-deoxyadenosine + cytosine
show the reaction diagram
Q9R5V5
-
-
-
?
2'-deoxycytidine + hypoxanthine
2'-deoxyinosine + cytosine
show the reaction diagram
-, Q6YNI5
-
-
-
?
2'-deoxycytidine + hypoxanthine
2'-deoxyinosine + cytosine
show the reaction diagram
Q9RSV5
-
-
-
?
2'-deoxycytidine + thymine
2'-deoxythymidine + cytosine
show the reaction diagram
Q6YNI5
-
-
-
?
2'-deoxyguanosine + thymine
2'-deoxythymidine + guanine
show the reaction diagram
-, Q6YNI5
-
-
-
?
2'-deoxyguanosine + thymine
2'-deoxythymidine + guanine
show the reaction diagram
Q9RSV5
-
-
-
?
2'-deoxyinosine + adenine
2'-deoxyadenosine + hypoxanthine
show the reaction diagram
-, Q6YNI5
-
-
-
?
2'-deoxyinosine + adenine
2'-deoxyadenosine + hypoxanthine
show the reaction diagram
Q9RSV5
-
-
-
?
2'-deoxyinosine + uracil
2'-deoxyuridine + hypoxanthine
show the reaction diagram
-
-
-
-
?
2'-deoxythymidine + adenine
2'-deoxyadenosine + thymine
show the reaction diagram
-, Q6YNI5
-
-
-
?
2'-deoxythymidine + adenine
2'-deoxyadenosine + thymine
show the reaction diagram
Q6YNI5
-
-
-
?
2'-deoxythymidine + adenine
2'-deoxyadenosine + thymine
show the reaction diagram
Q9RSV5
-
-
-
?
2'-deoxyuridine + 2,6-diaminopurine
2,6-diaminopurine 2-deoxy-D-ribose 5-phosphate + uracil
show the reaction diagram
A5VK58, -
-
-
-
?
2'-deoxyuridine + 2,6-diaminopurine
2,6-diaminopurine 2-deoxy-D-ribose 5-phosphate + uracil
show the reaction diagram
-
-
-
-
?
2'-deoxyuridine + 5-azacytosine
5-aza-2'-deoxycytidine + uracil
show the reaction diagram
-
-
-
-
?
2'-deoxyuridine + 5-ethyluracil
5-ethyl-2'-deoxyuridine + uracil
show the reaction diagram
-
-
-
-
?
2'-deoxyuridine + 5-fluorocytosine
2'-deoxy-D-ribosyl-5-flurocytidine + cytosine
show the reaction diagram
A5VK58, -
-
-
-
?
2'-deoxyuridine + 5-fluorocytosine
2'-deoxy-D-ribosyl-5-flurocytidine + cytosine
show the reaction diagram
-
-
-
-
?
2'-deoxyuridine + 5-methylcytosine
5-methyl-2'-deoxycytidine + uracil
show the reaction diagram
-
-
-
-
?
2'-deoxyuridine + 6-mercaptopurine
6-mercaptopurine 2-deoxy-D-ribose 5-phosphate + uracil
show the reaction diagram
A5VK58, -
-
-
-
?
2'-deoxyuridine + 6-mercaptopurine
6-mercaptopurine 2-deoxy-D-ribose 5-phosphate + uracil
show the reaction diagram
-
-
-
-
?
2'-deoxyuridine + adenine
2'-deoxyadenosine + uracil
show the reaction diagram
-
-
-
-
?
2'-deoxyuridine + benzimidazole
benzimidazole 2-deoxy-D-ribose 5-phosphate + uracil
show the reaction diagram
A5VK58, -
-
-
-
?
2'-deoxyuridine + benzimidazole
benzimidazole 2-deoxy-D-ribose 5-phosphate + uracil
show the reaction diagram
-
-
-
-
?
2'-deoxyuridine + cytosine
2'-deoxycytidine + uracil
show the reaction diagram
-, Q6YNI5
-
-
-
?
2'-deoxyuridine + cytosine
2'-deoxycytidine + uracil
show the reaction diagram
Q9RSV5
-
-
-
?
2'-deoxyuridine + cytosine
2'-deoxycytidine + uracil
show the reaction diagram
A5VK58, -
-
-
-
?
2'-deoxyuridine + cytosine
2'-deoxycytidine + uracil
show the reaction diagram
-
-
-
-
?
2'-deoxyuridine + hypoxanthine
2'-deoxyinosine + uracil
show the reaction diagram
-
-
-
-
?
5'-fluoro-5'-thymidine + adenine
5'-fluoro-5'-deoxythymine + adenosine
show the reaction diagram
-
deoxyribosyltransferase II
-
-
?
adenine + deoxycytidine
deoxyadenosine + cytosine
show the reaction diagram
-
64.0% conversion after 4 h at standard reaction conditions: 2 mM each substrate, 0.013 U NTD, pH 7.5, 25C, 40 mM (NH4)2SO4, 0.5 mg/ml bovine serum albumin
-
-
r
adenine + deoxyguanosine
deoxyadenosine + guanine
show the reaction diagram
-
46.6% conversion after 4 h at standard reaction conditions: 2 mM each substrate, 0.013 U NTD, pH 7.5, 25C, 40 mM (NH4)2SO4, 0.5 mg/ml bovine serum albumin
-
-
r
adenine + deoxyinosine
deoxyadenosine + hypoxanthine
show the reaction diagram
-
57.3% conversion after 4 h at standard reaction conditions: 2 mM each substrate, 0.013 U NTD, pH 7.5, 25C, 40 mM (NH4)2SO4, 0.5 mg/ml bovine serum albumin
-
-
r
adenine + deoxyuridine
deoxyadenosine + uracil
show the reaction diagram
-
75.4% conversion after 4 h at standard reaction conditions: 2 mM each substrate, 0.013 U NTD, pH 7.5, 25C, 40 mM (NH4)2SO4, 0.5 mg/ml bovine serum albumin
-
-
r
adenine + thymidine
deoxyadenosine + thymine
show the reaction diagram
-
74.6% conversion after 4 h at standard reaction conditions: 2 mM each substrate, 0.013 U NTD, pH 7.5, 25C, 40 mM (NH4)2SO4, 0.5 mg/ml bovine serum albumin
-
-
r
cytosine + deoxyadenosine
deoxycytidine + adenine
show the reaction diagram
-
27.7% conversion after 4 h at standard reaction conditions: 2 mM each substrate, 0.013 U NTD, pH 7.5, 25C, 40 mM (NH4)2SO4, 0.5 mg/ml bovine serum albumin
-
-
r
cytosine + deoxyguanosine
deoxycytidine + guanine
show the reaction diagram
-
25.7% conversion after 4 h at standard reaction conditions: 2 mM each substrate, 0.013 U NTD, pH 7.5, 25C, 40 mM (NH4)2SO4, 0.5 mg/ml bovine serum albumin
-
-
r
cytosine + deoxyinosine
deoxycytidine + hypoxanthine
show the reaction diagram
-
32.0% conversion after 4 h at standard reaction conditions: 2 mM each substrate, 0.013 U NTD, pH 7.5, 25C, 40 mM (NH4)2SO4, 0.5 mg/ml bovine serum albumin
-
-
r
cytosine + deoxyuridine
deoxycytidine + uracil
show the reaction diagram
-
62.9% conversion after 4 h at standard reaction conditions: 2 mM each substrate, 0.013 U NTD, pH 7.5, 25C, 40 mM (NH4)2SO4, 0.5 mg/ml bovine serum albumin
-
-
r
cytosine + thymidine
deoxycytidine + thymine
show the reaction diagram
-
53.5% conversion after 4 h at standard reaction conditions: 2 mM each substrate, 0.013 U NTD, pH 7.5, 25C, 40 mM (NH4)2SO4, 0.5 mg/ml bovine serum albumin, standard reaction for cofactor analyses as well as activating compound and inhibitor studies, in presence of 40 mM ammonium sulfate and 0.5 mg/ml bovine serum albumin
-
-
r
dAMP + base
dNMP + adenine
show the reaction diagram
-
-
-
-
r
dCMP + base
dNMP + cytidine
show the reaction diagram
-
-
-
-
r
deoxyadenosine + 2,6-dichloropurine
adenine + 9-(2-deoxy-beta-D-ribofuranosyl)-2,6-dichloropurine
show the reaction diagram
-
-
-
-
?
deoxyadenosine + 2-amino 6-methylmercaptopurine
adenine + 9-(2-deoxy-beta-D-ribofuranosyl)-2-amino-6-methylmercaptopurine
show the reaction diagram
-
-
-
-
?
deoxyadenosine + 2-amino-6-hydroxy 8-mercaptopurine
adenine + ?
show the reaction diagram
-
-
-
-
?
deoxyadenosine + 2-aminobenzimidazole
adenine + ?
show the reaction diagram
-
-
-
-
?
deoxyadenosine + 2-hydroxybenzimidazole
adenine + ?
show the reaction diagram
-
-
-
-
?
deoxyadenosine + 2-thiouracil
adenine + deoxy-2-thiouridine
show the reaction diagram
-
-
-
-
?
deoxyadenosine + 4-amino-5-imidazole carboxamide
adenine + ?
show the reaction diagram
-
-
-
-
?
deoxyadenosine + 5-fluorouracil
adenine + deoxy-5-fluorouridine
show the reaction diagram
-
-
-
-
?
deoxyadenosine + 5-iodouracil
adenine + deoxy-5-iodouridine
show the reaction diagram
-
-
-
-
?
deoxyadenosine + 6-azathymine
adenine + deoxy-6-deazathymidine
show the reaction diagram
-
-
-
-
?
deoxyadenosine + 6-chloroguanine
adenine + 2'-deoxy-6-chloroguanosine
show the reaction diagram
-
-
-
-
?
deoxyadenosine + 6-chloropurine
adenine + 9-(2-deoxy-beta-D-ribofuranosyl)-6-chloropurine
show the reaction diagram
-
-
-
-
?
deoxyadenosine + 6-dimethylaminopurine
adenine + 9-(2-deoxy-beta-D-ribofuranosyl)-6-dimethylaminopurine
show the reaction diagram
-
-
-
-
?
deoxyadenosine + 6-methylpurine
adenine + 9-(2-deoxy-beta-D-ribofuranosyl)-6-methylpurine
show the reaction diagram
-
-
-
-
?
deoxyadenosine + adenine
adenine + deoxyadenosine
show the reaction diagram
-
-
-
-
?
deoxyadenosine + cytosine
adenine + deoxycytidine
show the reaction diagram
-
-
-
-
r
deoxyadenosine + cytosine
adenine + deoxycytidine
show the reaction diagram
-
DRTase II
-
-
?
deoxyadenosine + ethyl 4-amino-1H-imidazole-5-carboxylate
adenine + ethyl 4-amino-1-(2-deoxy-b-D-erythro-pentofuranosyl)-1H-imidazole-5-carboxylate
show the reaction diagram
-
-
-
-
?
deoxyadenosine + hypoxanthine
adenine + deoxyinosine + 7-(beta-D-2'-deoxyribufuranosyl)hypoxanthine
show the reaction diagram
-
-
-
?
deoxyadenosine + hypoxanthine
adenine + deoxyinosine + 7-(beta-D-2'-deoxyribufuranosyl)hypoxanthine
show the reaction diagram
-
-
-
-
r
deoxyadenosine + thymine
adenine + thymidine
show the reaction diagram
-
-
-
-
r
deoxyadenosine + uracil
adenine + deoxyuridine
show the reaction diagram
-
-
-
-
r
deoxyadenosine + xanthine
adenine + deoxyxanthosine
show the reaction diagram
-
-
-
-
r
deoxycytidine + 1,7-dimethylguanine
cytosine + deoxy-1,7-dimethylguanosine
show the reaction diagram
-
DRTase II
-
-
?
deoxycytidine + 1,N2-epsilon-guanine
cytosine + ?
show the reaction diagram
-
deoxyribosylation at the N9 atom
-
-
?
deoxycytidine + 1,N6-epsilon-adenine
cytosine + ?
show the reaction diagram
-
deoxyribosylation at the N9 atom
-
-
?
deoxycytidine + 3,N4-epsilon-cytosine
cytosine + ?
show the reaction diagram
-
deoxyribosylation at the N9 atom
-
-
?
deoxycytidine + 5,6,7,9-tetrahydro-7-acetoxy-9-oxoimidazo[1,2-a]purine
cytosine + ?
show the reaction diagram
-
deoxyribosylation at the N9 atom
-
-
?
deoxycytidine + 6-chloroguanine
hypoxanthine + deoxy-6-chloroguanosine
show the reaction diagram
-
DRTase II
-
-
?
deoxycytidine + adenine
cytosine + deoxyadenosine
show the reaction diagram
-
-
-
-
r
deoxycytidine + adenine
cytosine + deoxyadenosine
show the reaction diagram
-
-
-
r
deoxycytidine + adenine
cytosine + deoxyadenosine
show the reaction diagram
-
-
-
r
deoxycytidine + adenine
cytosine + deoxyadenosine
show the reaction diagram
-
-
-
-
r
deoxycytidine + C8-aminoguanine
cytosine + 2'-deoxy-8-aminoguanosine
show the reaction diagram
-
deoxyribosylation at the N9 atom
-
-
?
deoxycytidine + C8-methylguanine
cytosine + 2'-deoxy-8-methylguanosine
show the reaction diagram
-
deoxyribosylation at the N9 atom
-
-
?
deoxycytidine + cytosine
cytosine + deoxycytidine
show the reaction diagram
-
-
-
-
?
deoxycytidine + guanine
cytosine + deoxyguanosine
show the reaction diagram
-
-
-
-
r
deoxycytidine + guanine
cytosine + deoxyguanosine
show the reaction diagram
-
-
-
-
r
deoxycytidine + hypoxanthine
cytosine + deoxyinosine
show the reaction diagram
-
-
-
-
r
deoxycytidine + N2,3-epsilon-guanine
cytosine + ?
show the reaction diagram
-
deoxyribosylation at the N9 atom
-
-
?
deoxycytidine + N2-(2-oxoethyl)guanine
cytosine + 2'-deoxy-N2-(2-oxoethyl)guanosine
show the reaction diagram
-
deoxyribosylation at the N9 atom
-
-
?
deoxycytidine + pyrimidopurin-10-one
cytosine + ?
show the reaction diagram
-
deoxyribosylation at the N9 atom
-
-
?
deoxycytidine + thymine
cytosine + thymidine
show the reaction diagram
-
-
-
-
r
deoxycytidine + xanthine
cytosine + xanthosine
show the reaction diagram
-
-
-
-
r
deoxyguanosine + adenine
guanine + deoxyadenosine
show the reaction diagram
-
-
-
-
?
deoxyguanosine + adenine
guanine + deoxyadenosine
show the reaction diagram
-
-
-
-
r
deoxyguanosine + cytosine
guanine + deoxycytidine
show the reaction diagram
-
-
-
-
-
deoxyguanosine + cytosine
guanine + deoxycytidine
show the reaction diagram
-
-
-
-
r
deoxyguanosine + hypoxanthine
guanine + deoxyinosine
show the reaction diagram
-
-
-
-
r
deoxyguanosine + thymine
guanine + thymidine
show the reaction diagram
-
-
-
-
r
deoxyguanosine + uracil
guanine + deoxyuridine
show the reaction diagram
-
-
-
-
r
deoxyguanosine + xanthine
guanine + deoxyxanthine
show the reaction diagram
-
-
-
-
r
deoxyinosine + 1,7-dimethylguanine
hypoxanthine + deoxy-1,7-dimethylguanosine
show the reaction diagram
-
DRTase I
-
-
?
deoxyinosine + 6-chloroguanine
hypoxanthine + deoxy-6-chloroguanosine
show the reaction diagram
-
DRTase I
-
-
?
deoxyinosine + 8-azaguanine
hypoxanthine + deoxy-8-azaguanosine
show the reaction diagram
-
-
-
-
r
deoxyinosine + 8-azaxanthine
hypoxanthine + deoxy-8-azaxanthosine
show the reaction diagram
-
-
-
-
r
deoxyinosine + adenine
hypoxanthine + deoxyadenosine
show the reaction diagram
-
-
-
r
deoxyinosine + adenine
hypoxanthine + deoxyadenosine
show the reaction diagram
-
-
-
r
deoxyinosine + adenine
hypoxanthine + deoxyadenosine
show the reaction diagram
-
-
-
-
r
deoxyinosine + adenine
hypoxanthine + deoxyadenosine
show the reaction diagram
-
DRTase I
-
-
r
deoxyinosine + cytosine
hypoxanthine + deoxycytidine
show the reaction diagram
-
-
-
-
?
deoxyinosine + cytosine
hypoxanthine + deoxycytidine
show the reaction diagram
-
-
-
-
r
deoxyinosine + cytosine
hypoxanthine + deoxycytidine
show the reaction diagram
-
DRTase II
-
-
?
deoxyinosine + guanine
hypoxanthine + deoxyguanosine
show the reaction diagram
-
-
-
?
deoxyinosine + guanine
hypoxanthine + deoxyguanosine
show the reaction diagram
-
-
-
r
deoxyinosine + guanine
hypoxanthine + deoxyguanosine
show the reaction diagram
-
DRTase I
-
-
-
deoxyinosine + thymine
hypoxanthine + thymidine
show the reaction diagram
-
-
-
-
r
deoxyinosine + thymine
hypoxanthine + thymidine
show the reaction diagram
-
DRTase II
-
-
-
deoxyinosine + uracil
hypoxanthine + deoxyuridine
show the reaction diagram
-
-
-
-
r
deoxyinosine + uric acid
hypoxanthine + 9-(2-deoxy-beta-D-ribofuranosyl)-7,9-dihydro-3H-purine-2,6,8-trione
show the reaction diagram
-
-
-
-
r
deoxyuridine + adenine
deoxyadenosine + uracil
show the reaction diagram
-
-
-
-
?
deoxyuridine + guanine
deoxyguanosine + uracil
show the reaction diagram
-
-
-
-
?
deoxyuridine + hypoxanthine
deoxyinosine + uracil
show the reaction diagram
-
-
-
-
?
dGMP + base
dNMP + guanine
show the reaction diagram
-
dGMP is the best substrate
-
-
r
dIMP + base
dNMP + hypoxanthine
show the reaction diagram
-
-
-
-
r
dTMP + base
dNMP + thymine
show the reaction diagram
-
-
-
-
r
dUMP + base
dNMP + uracil
show the reaction diagram
-
-
-
-
r
hypoxanthine + deoxyadenosine
deoxyinosine + adenine
show the reaction diagram
-
38.0% conversion after 4 h at standard reaction conditions: 2 mM substrate, 0.013 U NTD, pH 7.5, 25C, 40 mM (NH4)2SO4, 0.5 mg/ml bovine serum albumin
-
-
r
hypoxanthine + deoxycytidine
deoxyinosine + cytosine
show the reaction diagram
-
51.9% conversion after 4 h at standard reaction conditions: 2 mM each substrate, 0.013 U NTD, pH 7.5, 25C, 40 mM (NH4)2SO4, 0.5 mg/ml bovine serum albumin
-
-
r
hypoxanthine + deoxyguanosine
deoxyinosine + guanine
show the reaction diagram
-
25.2% conversion after 4 h at standard reaction conditions: 2 mM substrate, 0.013 U NTD, pH 7.5, 25C, 40 mM (NH4)2SO4, 0.5 mg/ml bovine serum albumin
-
-
r
hypoxanthine + deoxyuridine
deoxyinosine + uracil
show the reaction diagram
-
65.6% conversion after 4 h at standard reaction conditions: 2 mM substrate, 0.013 U NTD, pH 7.5, 25C, 40 mM (NH4)2SO4, 0.5 mg/ml bovine serum albumin
-
-
r
purine base + 2-deoxy-D-ribosyl-base
purine deoxyribonucleoside + base
show the reaction diagram
-
-
preferred over pyrimidine deoxyribonucleoside synthesis
-
r
pyrimidine base + 2-deoxy-D-ribosyl-base
pyrimidine deoxyribonucleoside + base
show the reaction diagram
-
-
-
-
r
thymidine + 1-deaza-8-aza-adenine
thymine + 2'-deoxy-1-deaza-8-aza-adenosine
show the reaction diagram
-
21% of substrate converted into deoxynucleoside after 2 h
-
-
?
thymidine + 1-deaza-adenine
thymine + 2'-deoxy-1-deaza-adenosine
show the reaction diagram
-
6.2% of substrate converted into deoxynucleoside after 2 h
-
-
?
thymidine + 2,6-diaminopurine
thymine + 9-(2-deoxy-beta-D-ribofuranosyl)-2,6-diaminopurine
show the reaction diagram
-
53.4% of substrate converted into deoxynucleoside after 2 h
-
-
?
thymidine + 2,6-dichloropurine
thymine + 9-(2-deoxy-beta-D-ribofuranosyl)-2,6-dichloropurine
show the reaction diagram
-
-
-
-
r
thymidine + 2-amino-6-methyl-mercaptopurine
thymine + 9-(2-deoxy-beta-D-ribofuranosyl)-2-amino-6-methylmercaptopurine
show the reaction diagram
-
-
-
-
r
thymidine + 2-pyrimidinol
thymine + ?
show the reaction diagram
-
-
-
-
?
thymidine + 2-thio-4-pyrimidinal
thymine + ?
show the reaction diagram
-
-
-
-
?
thymidine + 2-thio-5-methyl-4-pyrimidinol
thymine + ?
show the reaction diagram
-
-
-
-
?
thymidine + 3-deaza-8-aza-2-aminopurine
thymine + 9-(2-deoxy-beta-D-ribofuranosyl)-3-deaza-8-aza-aminopurine
show the reaction diagram
-
39.7% of substrate converted into deoxynucleoside after 2 h
-
-
?
thymidine + 3-deaza-8-aza-adenine
thymine + 2'-deoxy-3-deaza-8-aza-adenosine
show the reaction diagram
-
3.5% of substrate converted into deoxynucleoside after 2 h
-
-
?
thymidine + 4,6-pyrimidinediol
thymine + ?
show the reaction diagram
-
-
-
-
?
thymidine + 4-amino-2-pyrimidinethiol
thymine + ?
show the reaction diagram
-
-
-
-
?
thymidine + 4-amino-5-carboxamide-imidazole
thymine + ?
show the reaction diagram
-
12.3% of substrate converted into deoxynucleoside after 2 h
-
-
?
thymidine + 4-amino-6-pyrimidinol
thymine + ?
show the reaction diagram
-
-
-
-
?
thymidine + 4-amino-pyrazolopyrimidine
thymine + ?
show the reaction diagram
-
0.37% of substrate converted into deoxynucleoside after 2 h
-
-
?
thymidine + 4-methyl-2-pyrimidinol
thymine + ?
show the reaction diagram
-
-
-
-
?
thymidine + 4-pyrimidinol
thymine + ?
show the reaction diagram
-
-
-
-
?
thymidine + 5,6-dimethyl-benzimidazole
thymine + ?
show the reaction diagram
-
37.7% of substrate converted into deoxynucleoside after 2 h
-
-
?
thymidine + 5-bromouracil
thymine + 2'-deoxy-5-bromouridine
show the reaction diagram
-
-
-
-
?
thymidine + 5-fluorouracil
thymine + 2'-deoxy-5-fluorouridine
show the reaction diagram
-
-
-
-
?
thymidine + 5-hydroxyuracil
thymine + 2'-deoxy-5-hydroxyuridine
show the reaction diagram
-
-
-
-
?
thymidine + 5-methylcytosine
thymine + 2'-deoxy-5-methylcytosine
show the reaction diagram
-
-
-
-
?
thymidine + 5-nitrouracil
thymine + 2'-deoxy-5-nitrouridine
show the reaction diagram
-
-
-
-
?
thymidine + 5-thiouracil
thymine + 2'-deoxy-5-thiouridine
show the reaction diagram
-
-
-
-
?
thymidine + 6-benzylaminopurine
thymine + 9-(2-deoxy-beta-D-ribofuranosyl)-6-benzylaminopurine
show the reaction diagram
-
-
-
-
r
thymidine + 6-chloropurine
thymine + 9-(2-deoxy-beta-D-ribofuranosyl)-6-chloropurine
show the reaction diagram
-
-
-
-
r
thymidine + 6-iodopurine
thymine + 9-(2-deoxy-beta-D-ribofuranosyl)-6-iodopurine
show the reaction diagram
-
65.6% of substrate converted into deoxynucleoside after 2 h
-
-
?
thymidine + 6-mercaptoguanine
thymine + 6-mercaptoguanosine
show the reaction diagram
-
52.1% of substrate converted into deoxynucleoside after 2 h
-
-
?
thymidine + 6-mercatopurine
thymine + 9-(2-deoxy-beta-D-ribofuranosyl)-6-mercaptopurine
show the reaction diagram
-
56.5% of substrate converted into deoxynucleoside after 2 h
-
-
?
thymidine + 6-methoxypurine
thymine + 9-(2-deoxy-beta-D-ribofuranosyl)-6-methoxypurine
show the reaction diagram
-
-
-
-
r
thymidine + 6-methyl-cis-triazine-3,5-diol
thymine + ?
show the reaction diagram
-
-
-
-
?
thymidine + 6-methylaminopurine
thymine + 9-(2-deoxy-beta-D-ribofuranosyl)-6-methylaminopurine
show the reaction diagram
-
-
-
-
r
thymidine + 6-methylpurine
thymine + 9-(2-deoxy-beta-D-ribofuranosyl)-6-methylpurine
show the reaction diagram
-
-
-
-
r
thymidine + 6-methylthio-8-trifluoromethylpurine
thymine + 9-(2-deoxy-beta-D-ribofuranosyl)-6-methylthio-8-trifluoromethylpurine
show the reaction diagram
-
-
-
-
?
thymidine + 6-n-hexylaminopurine
thymine + 9-(2-deoxy-beta-D-ribofuranosyl)-6-n-hexylaminopurine
show the reaction diagram
-
-
-
-
r
thymidine + 6-n-hexylmercaptopurine
thymine + 9-(2-deoxy-beta-D-ribofuranosyl)-6-n-hexylmercaptopurine
show the reaction diagram
-
-
-
-
r
thymidine + 6-phenylaminopurine
thymine + 9-(2-deoxy-beta-D-ribofuranosyl)-6-phenylaminopurine
show the reaction diagram
-
-
-
-
r
thymidine + 7-hydroxy-1,2,5-selenadiazolopyridine
thymine + ?
show the reaction diagram
-
29.2% of substrate converted into deoxynucleoside after 2 h
-
-
?
thymidine + 8-aza-2,6-diaminopurine
thymine + 9-(2-deoxy-beta-D-ribofuranosyl)-8-aza-2,6-diaminopurine
show the reaction diagram
-
59.6% of substrate converted into deoxynucleoside after 2 h
-
-
?
thymidine + 8-azaguanine
thymine + 2'-deoxy-8-azaguanosine
show the reaction diagram
-
16.0% of substrate converted into deoxynucleoside after 2 h
-
-
?
thymidine + 8-azaxanthine
thymine + 2'-deoxy-8-azaxanthosine
show the reaction diagram
-
-
-
-
?
thymidine + 8-bromoadenine
thymine + 2'-deoxy-8-bromoadenosine
show the reaction diagram
-
-
-
?
thymidine + 8-chloroadenine
thymine + 2'-deoxy-8-chloroadenosine
show the reaction diagram
-
-
3-deoxyribonucleoside + 9-deoxyribonucleoside
?
thymidine + 8-chlorotheophylline
thymine + 9-(2-deoxy-beta-D-ribofuranosyl)-8-chlorotheophylline
show the reaction diagram
-
-
-
-
?
thymidine + 8-methyladenine
thymine + 2'-deoxy-8-methyladenosine
show the reaction diagram
-
-
-
-
?
thymidine + 8-trifluoromethyladenine
thymine + 2'-deoxy-8-trifluoromethyladenosine
show the reaction diagram
-
-
-
-
?
thymidine + adenine
thymine + adenosine
show the reaction diagram
-
-
-
-
?
thymidine + adenine
thymine + adenosine
show the reaction diagram
-
-
3-deoxyribonucleoside
?
thymidine + adenine
thymine + adenosine
show the reaction diagram
-
DRTase II
-
-
?
thymidine + adenine
thymine + 2'-deoxyadenosine
show the reaction diagram
-
-
-
-
r
thymidine + adenine
2'-deoxy-D-ribosyl-adenine + thymine
show the reaction diagram
Bacillus coagulans, Lactobacillus sp., Psychrobacter immobilis, Bacillus psychrosaccharolyticus, Lactobacillus sp. CECT 4219, Bacillus psychrosaccharolyticus CECT 4074, Psychrobacter immobilis CECT 4492, Bacillus coagulans CECT 12
-
-
-
-
r
thymidine + adenine
2'-deoxyadenosine + thymine
show the reaction diagram
-
-
-
-
?
thymidine + adenine
2'-deoxyadenosine + thymine
show the reaction diagram
-
-
-
-
?
thymidine + adenine
2'-deoxyadenosine + thymine
show the reaction diagram
Lactobacillus fermentum CGMCC 1.2133
-
-
-
-
?
thymidine + benzimidazole
thymine + ?
show the reaction diagram
-
33.1% of substrate converted into deoxynucleoside after 2 h
-
-
?
thymidine + cis-triazine-3,5-diol
thymine + ?
show the reaction diagram
-
-
-
-
?
thymidine + cytosine
thymine + 2'-deoxycytidine
show the reaction diagram
-
-
-
-
?
thymidine + cytosine
thymine + 2'-deoxycytidine
show the reaction diagram
-
-
-
-
?
thymidine + guanine
thymine + 2'-deoxyguanosine
show the reaction diagram
-
-
-
-
?
thymidine + guanine
thymine + 2'-deoxyguanosine
show the reaction diagram
-
DRTase II
-
-
?
thymidine + guanine
thymine + 2'-deoxyguanosine
show the reaction diagram
-
42.3% of substrate converted into deoxynucleoside after 2 h
-
-
?
thymidine + guanine
deoxyguanine + thymine
show the reaction diagram
-
-
-
-
?
thymidine + hypoxanthine
deoxyinosine + thymine
show the reaction diagram
-
-
-
-
?
thymidine + hypoxanthine
thymine + 2'-deoxyinosine
show the reaction diagram
-
-
-
-
r
thymidine + purine
thymine + 9-(2-deoxy-beta-D-ribofuranosyl)-purine
show the reaction diagram
-
-
-
-
r
thymidine + theophylline
thymine + 2'-deoxytheophylline riboside
show the reaction diagram
-
-
-
-
?
thymidine + uracil
deoxyuridine + thymine
show the reaction diagram
-
-
-
-
?
thymidine + uracil
thymine + 2'-deoxyuridine
show the reaction diagram
-
-
-
-
?
thymidine + xanthine
9-deoxyribosylxanthine + 7-deoxyribosylxanthine
show the reaction diagram
-
-
20% 9-deoxyribosylxanthine + 80% 7-deoxyribosylxanthine
?
thymidine + xanthine
thymine + xanthosine
show the reaction diagram
-
-
-
-
r
thymine + deoxyadenosine
thymidine + adenine
show the reaction diagram
-
22.7% conversion after 4 h at standard reaction conditions: 2 mM each substrate, 0.013 U NTD, pH 7.5, 25C, 40 mM (NH4)2SO4, 0.5 mg/ml bovine serum albumin
-
-
r
thymine + deoxycytidine
thymidine + cytosine
show the reaction diagram
-
38.3% conversion after 4 h at standard reaction conditions: 2 mM each substrate, 0.013 U NTD, pH 7.5, 25C, 40 mM (NH4)2SO4, 0.5 mg/ml bovine serum albumin
-
-
r
thymine + deoxyguanosine
thymidine + guanine
show the reaction diagram
-
17.7% conversion after 4 h at standard reaction conditions: 2 mM each substrate, 0.013 U NTD, pH 7.5, 25C, 40 mM (NH4)2SO4, 0.5 mg/ml bovine serum albumin
-
-
r
thymine + deoxyinosine
thymidine + hypoxanthine
show the reaction diagram
-
19.5% conversion after 4 h at standard reaction conditions: 2 mM each substrate, 0.013 U NTD, pH 7.5, 25C, 40 mM (NH4)2SO4, 0.5 mg/ml bovine serum albumin, equilibrium reached after 2 h
-
-
r
thymine + deoxyuridine
thymidine + uracil
show the reaction diagram
-
53.7% conversion after 4 h at standard reaction conditions: 2 mM each substrate, 0.013 U NTD, pH 7.5, 25C, 40 mM (NH4)2SO4, 0.5 mg/ml bovine serum albumin
-
-
r
uracil + deoxyadenosine
deoxyuridine + adenine
show the reaction diagram
-
17.1% conversion after 4 h at standard reaction conditions: 2 mM each substrate, 0.013 U NTD, pH 7.5, 25C, 40 mM (NH4)2SO4, 0.5 mg/ml bovine serum albumin
-
-
r
uracil + deoxycytidine
deoxyuridine + cytosine
show the reaction diagram
-
37.7% conversion after 4 h at standard reaction conditions: 2 mM each substrate, 0.013 U NTD, pH 7.5, 25C, 40 mM (NH4)2SO4, 0.5 mg/ml bovine serum albumin
-
-
r
uracil + deoxyguanosine
deoxyuridine + guanine
show the reaction diagram
-
16.1% conversion after 4 h at standard reaction conditions: 2 mM each substrate, 0.013 U NTD, pH 7.5, 25C, 40 mM (NH4)2SO4, 0.5 mg/ml bovine serum albumin
-
-
r
uracil + deoxyinosine
deoxyuridine + hypoxanthine
show the reaction diagram
-
23.8% conversion after 4 h at standard reaction conditions: 2 mM each substrate, 0.013 U NTD, pH 7.5, 25C, 40 mM (NH4)2SO4, 0.5 mg/ml bovine serum albumin
-
-
r
uracil + thymidine
deoxyuridine + thymine
show the reaction diagram
-
43.0% conversion after 4 h at standard reaction conditions: 2 mM each substrate, 0.013 U NTD, pH 7.5, 25C, 40 mM (NH4)2SO4, 0.5 mg/ml bovine serum albumin
-
-
r
hypoxanthine + thymidine
deoxyinosine + thymine
show the reaction diagram
-
76.8% conversion after 4 h at standard reaction conditions: 2 mM substrate, 0.013 U NTD, pH 7.5, 25C, 40 mM (NH4)2SO4, 0.5 mg/ml bovine serum albumin
-
-
r
additional information
?
-
-
nucleoside deoxyribosyltransferase I is strictly specific for transfer between purine bases. Nucleoside deoxyribosyltransferase II catalyzes the transfer of the deoxyribosyl moiety between purines or pyrimidines as well as from a purine to a pyrimidine
-
-
-
additional information
?
-
-
DRTase I is strictly specific for transfer between purine bases. DRTase II catalyzes the transfer of the deoxyribosyl moiety between purines or pyrimidines as well as from a purine to a pyrimidine
-
-
-
additional information
?
-
Q8RLY5
DRTase I is strictly specific for transfer between purine bases. DRTase II catalyzes the transfer of the deoxyribosyl moiety between purines or pyrimidines as well as from a purine to a pyrimidine, DRTase I is specific for purines with preference for deoxyinosine, deoxyadenosine, deoxyguanosine as donor substrates
-
-
-
additional information
?
-
-
the enzyme participates in the nucleoside salvage pathway
-
-
-
additional information
?
-
Q8RLY5
specific role for DRTase I in the metabolism of deoxyinosine
-
-
-
additional information
?
-
-
the enzyme is important in the salvage pathway of nucleoside recycling, Trypanosoma brucei lacks enzymes of de novo purine biosynthesis
-
-
-
additional information
?
-
-
RCL cleaves the N-glycosidic bond of deoxynucleoside 5'-monophosphates to yield two reaction products, deoxyribose 5-phosphate and purine or pyrimidine base
-
-
-
additional information
?
-
-
the enzyme is selective for 6-aminopurine compared to 6-oxopurines and for 2'-deoxyuridine compared to thymidine
-
-
-
additional information
?
-
-
preferably purine 2-deoxyribonucleoside synthesis compared to pyrimidine deoxyribonucleoside synthesis although both are catalysed, ribonucleoside, arabinosylnucleoside and 2,3-dideoxyribonucleoside are no substrates for base-exchange by YejD
-
-
-
additional information
?
-
A5VK58, -
enzyme does not interchange bases between ribonucleosides, but is able to catalyze the transfer of 2'-deoxyribose between purine or pyrimidine and/or pyrimidine bases. The enzyme prefers pyrimidines to purines as substrates, dUrd being the best nucleoside donor, followed by dThd, dAdo, and dIno, whereas cytosine is the best base acceptor and hypoxanthine is the worst
-
-
-
additional information
?
-
Lactobacillus sp. CECT 4219, Bacillus psychrosaccharolyticus CECT 4074, Psychrobacter immobilis CECT 4492, Bacillus coagulans CECT 12
-
the enzyme is selective for 6-aminopurine compared to 6-oxopurines and for 2'-deoxyuridine compared to thymidine
-
-
-
thymidine + 2-aminopurine
thymine + 9-(2-deoxy-beta-D-ribofuranosyl)-2-aminopurine
show the reaction diagram
-
-
-
-
r
thymidine + 2-hydroxy-6-mercaptopurine
additional information
-
-
-
2 products
r
thymidine + 2-mercaptoadenine
additional information
-
-
-
2 products
r
thymidine + 2-mercaptopurine
additional information
-
-
-
2 products
r
thymidine + 6-dimethylaminopurine
thymine + 9-(2-deoxy-beta-D-ribofuranosyl)-6-dimethylaminopurine
show the reaction diagram
-
-
-
-
r
thymidine + 6-hydrazinepurine
additional information
-
-
-
several products observed
r
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
(Substrate)
LITERATURE
(Substrate)
COMMENTARY
(Product)
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
purine base + 2-deoxy-D-ribosyl-base
purine deoxyribonucleoside + base
show the reaction diagram
-
-
preferred over pyrimidine deoxyribonucleoside synthesis
-
r
pyrimidine base + 2-deoxy-D-ribosyl-base
pyrimidine deoxyribonucleoside + base
show the reaction diagram
-
-
-
-
r
2'-deoxy-D-ribosyl-uracil + adenine
2'-deoxy-D-ribosyl-adenine + uracil
show the reaction diagram
Bacillus coagulans, Lactobacillus sp., Psychrobacter immobilis, Bacillus psychrosaccharolyticus, Lactobacillus sp. CECT 4219, Bacillus psychrosaccharolyticus CECT 4074, Psychrobacter immobilis CECT 4492, Bacillus coagulans CECT 12
-
-
-
-
r
additional information
?
-
-
the enzyme participates in the nucleoside salvage pathway
-
-
-
additional information
?
-
Q8RLY5
specific role for DRTase I in the metabolism of deoxyinosine
-
-
-
additional information
?
-
-
the enzyme is important in the salvage pathway of nucleoside recycling, Trypanosoma brucei lacks enzymes of de novo purine biosynthesis
-
-
-
additional information
?
-
-
preferably purine 2-deoxyribonucleoside synthesis compared to pyrimidine deoxyribonucleoside synthesis although both are catalysed
-
-
-
METALS and IONS
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
NH4+
-
enhances base exchange activity and thermostability, 1.43-fold activation by 1 mM (NH4)2SO4 (reaches plateau at 20 mM)
Rb+
-
enhances base exchange activity, 1.51-fold activation by 1 mM RbCl
K+
-
enhances base exchange activity and thermostability, 1.66-fold activation by 1 mM KCl (reaches plateau at 20 mM)
additional information
-
no effect by Ca2+, Mg2+, Na+, and K+
additional information
-
enzymatic activity depends on monovalent cations as cofactors rather than catalysts; K+ and NH4+ have no synergistic effects; Li+ and Na+ do not activate
INHIBITORS
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
IMAGE
6-methylthio-GMP
-
competitive versus dGMP
amino-pyrrolo-pyrimidine
-
12.1 mM, complete inhibition of the reaction with adenine and deoxycytidine
Ca2+
-
inhibits base exchange activity at 1 mM
CO2
-
reduces base exchange activity by 87-99% at 1 mM
Cu2+
-
reduces base exchange activity by 87-99% at 1 mM
deoxyadenosine
-
linear competitive inhibition of deoxyinosine and linear non-competitive inhibition of adenine
deoxyadenosine
-
linear competitive inhibition of deoxycytidine
GMP
-
competitive versus dGMP
Hydroxypyrrolopyrimidine
-
0.33 mM, 66% inhibition of the reaction with adenine and deoxycytidine
-
imidazole
-
12.1 mM, 76% inhibition of the reaction with adenine and deoxycytidine
Mg2+
-
inhibits base exchange activity at 1mM
Mn2+
-
reduces base exchange activity by 87-99% at 1 mM
NaCl
-
70% activity remains at 1 M NaCl
Tris
-
complete inhibition of deoxyribosyltransferase I at 0.1 M, pH 6.9. 62% inhibition of nucleoside deoxyribosyltransferase I at 0.8 M, pH 5.9
Zn2+
-
reduces base exchange activity by 87-99% at 1 mM
ACTIVATING COMPOUND
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
IMAGE
K+
-
K+-activated type II enzyme: binding of K+ is required to enhance activity through induced conformational change
additional information
-
does not require phosphate derivatives such as ATP, thiamine diphosphate, pyridoxal phosphate to complete 2-deoxyribose transfer
-
additional information
-
no activation observed with NH4+, K+ or Rb+
-
KM VALUE [mM]
KM VALUE [mM] Maximum
SUBSTRATE
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
IMAGE
0.38
-
2',3'-dideoxycytidine
-, Q6YNI5
wild-type
0.8
-
2',3'-dideoxycytidine
-, Q6YNI5
mutant A15T
0.92
-
2',3'-dideoxycytidine
Q9RSV5
mutant G9S
0.25
-
2'-deoxycytidine
Q9RSV5
wild-type
0.3
-
2'-deoxycytidine
-, Q6YNI5
wild-type
0.3
-
2'-deoxycytidine
Q9RSV5
mutant G9S
0.39
-
2'-deoxycytidine
-, Q6YNI5
mutant A15T
11
-
2'-deoxycytidine
Q9R5V5
mutant F13R/D92S/N123S, pH 6.0, 37C
22
-
2'-deoxycytidine
Q9R5V5
mutant F13R/D92S, pH 6.0, 37C
1.14
-
2'-Deoxyuridine
-
pH 6.5, 40C
0.0073
-
adenine
-
reaction with deoxyinosine
0.008
-
adenine
-
reaction with deoxyguanosine
0.019
-
adenine
-
reaction with deoxycytidine
0.021
-
adenine
-
reaction with deoxyadenosine
0.3
-
adenine
-
reaction with deoxycytidine
0.48
-
adenine
-
reaction with thymidine
0.04
-
adenosine
-
reaction with deoxyinosine
0.05
-
cytosine
-
reaction with thymidine
0.073
-
cytosine
-
reaction with deoxyinosine
0.077
-
cytosine
-
reaction with deoxyguanosine
0.12
-
cytosine
-
reaction with deoxyadenosine
0.17
-
cytosine
-
reaction with deoxycytidine
0.18
-
cytosine
-
reaction with deoxyinosine
0.19
-
cytosine
-
reaction with deoxyguanosine
0.22
-
cytosine
-
reaction with deoxyadenosine
10.79
-
cytosine
-
pH 6.5, 40C
0.25
-
dAMP
-
pH 7.5, 37C, recombinant wild-type enzyme
4
-
dCMP
-
pH 7.5, 37C, recombinant wild-type enzyme
0.092
-
deoxyadenosine
-
reaction with adenine
0.1
-
deoxyadenosine
-
reaction with cytosine
0.12
-
deoxyadenosine
-
-
0.12
-
deoxyadenosine
-
reaction with cytosine
0.45
-
deoxyadenosine
-
-
0.09
-
Deoxycytidine
-
-
0.09
-
Deoxycytidine
-
reaction with adenine
0.095
-
Deoxycytidine
-
reaction with cytosine
0.52
-
Deoxycytidine
-
reaction with guanine
0.55
-
Deoxycytidine
-
reaction with adenine
0.16
-
deoxyguanosine
-
reaction with cytosine
0.346
-
deoxyguanosine
-
reaction with adenine
0.37
-
deoxyguanosine
-
reaction with cytosine
0.041
-
Deoxyinosine
-
-
0.27
-
Deoxyinosine
-
reaction with guanine
0.3
-
Deoxyinosine
-
reaction with thymine
0.33
-
Deoxyinosine
-
reaction with adenosine
0.35
-
Deoxyinosine
-
-
0.35
-
Deoxyinosine
-
reaction with cytosine
3.4
-
Deoxyinosine
-
reaction with adenine
3.5
-
Deoxyinosine
-
reaction with cytosine
0.048
-
dGMP
-
pH 7.5, 37C, recombinant wild-type enzyme
5
-
dGMP
-
pH 7.5, 37C, recombinant mutant E105A
0.45
-
dIMP
-
pH 7.5, 37C, recombinant wild-type enzyme
50
-
dTMP
-
above, pH 7.5, 37C, recombinant wild-type enzyme
15
-
dUMP
-
pH 7.5, 37C, recombinant wild-type enzyme
0.03
-
Guanine
-
reactioin with deoxyinosine
0.48
-
Guanine
-
reaction with deoxycytidine
0.52
-
Guanine
-
reaction with thymidine
0.05
-
thymidine
-
reaction with cytosine
0.51
-
thymidine
-
reaction with adenine
0.63
-
thymidine
-
reaction with guanine
0.13
-
thymine
-
reaction with deoxyinosine
0.086
-
hypoxanthine
-
-
additional information
-
additional information
-
kinetics and substrate specifiicty
-
TURNOVER NUMBER [1/s]
TURNOVER NUMBER MAXIMUM[1/s]
SUBSTRATE
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
IMAGE
0.047
-
2',3'-dideoxycytidine
-, Q6YNI5
wild-type
2.54
-
2',3'-dideoxycytidine
Q9RSV5
mutant G9S
10.4
-
2',3'-dideoxycytidine
-, Q6YNI5
mutant A15T
0.3
-
2'-deoxycytidine
Q9R5V5
mutant F13R/D92S, pH 6.0, 37C
2.1
-
2'-deoxycytidine
Q9R5V5
mutant F13R/D92S/N123S, pH 6.0, 37C
5.36
-
2'-deoxycytidine
-, Q6YNI5
mutant A15T
12.4
-
2'-deoxycytidine
Q9RSV5
mutant G9S
45.1
-
2'-deoxycytidine
-, Q6YNI5
wild-type
62.2
-
2'-deoxycytidine
Q9RSV5
wild-type
483
-
2'-Deoxyuridine
-
pH 6.5, 40C
2083
-
cytosine
-
pH 6.5, 40C
0.0066
-
dAMP
-
pH 7.5, 37C, recombinant wild-type enzyme
0.0231
-
dCMP
-
pH 7.5, 37C, recombinant wild-type enzyme
0.0178
-
dGMP
-
pH 7.5, 37C, recombinant mutant E105A
0.0297
-
dGMP
-
pH 7.5, 37C, recombinant wild-type enzyme
0.0066
-
dIMP
-
pH 7.5, 37C, recombinant wild-type enzyme
0.158
-
dUMP
-
pH 7.5, 37C, recombinant wild-type enzyme
additional information
-
additional information
-
-
-
kcat/KM VALUE [1/mMs-1]
kcat/KM VALUE [1/mMs-1] Maximum
SUBSTRATE
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
IMAGE
0.12
-
2',3'-dideoxycytidine
-, Q6YNI5
wild-type
209400
9.1
-
2',3'-dideoxycytidine
Q9RSV5
mutant G9S
209400
13
-
2',3'-dideoxycytidine
-, Q6YNI5
mutant A15T
209400
0.0136
-
2'-deoxycytidine
Q9R5V5
mutant F13R/D92S, pH 6.0, 37C
1646
0.192
-
2'-deoxycytidine
Q9R5V5
mutant F13R/D92S/N123S, pH 6.0, 37C
1646
14
-
2'-deoxycytidine
-, Q6YNI5
mutant A15T
1646
41
-
2'-deoxycytidine
Q9RSV5
mutant G9S
1646
150
-
2'-deoxycytidine
-, Q6YNI5
wild-type
1646
250
-
2'-deoxycytidine
Q9RSV5
wild-type
1646
424
-
2'-Deoxyuridine
-
pH 6.5, 40C
1656
193
-
cytosine
-
pH 6.5, 40C
8956
Ki VALUE [mM]
Ki VALUE [mM] Maximum
INHIBITOR
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
IMAGE
0.01
-
6-methylthio-GMP
-
pH 7.5, 37C, recombinant wild-type enzyme, versus dGMP
0.092
-
deoxyadenosine
-
-
0.43
-
deoxyadenosine
-
-
0.095
-
Deoxycytidine
-
-
0.34
-
Deoxyinosine
-
-
0.02
-
GMP
-
pH 7.5, 37C, recombinant wild-type enzyme, versus dGMP
0.079
-
hypoxanthine
-
-
SPECIFIC ACTIVITY [µmol/min/mg]
SPECIFIC ACTIVITY MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
0.01
-
-, Q6YNI5
wild-type, with 2',3'-dideoxyguanosine and thymine as substrates; wild-type, with 2',3'-dideoxyinosine and adenine as substrates
0.01
-
Q9RSV5
wild-type, with 2',3'didehydro-2',3'-dideoxythymidine and adenine as substrates; wild-type, with 2',3'-dideoxyguanosine and thymine as substrates; wild-type, with 2',3'-dideoxyinosine and adenine as substrates; wild-type, with 2'-deoxycytidine and hypoxanthine as substrates
0.01
-
Q6YNI5
wild-type, 2',3'-dideoxyadenosine and thymine as substrates; wild-type, 2',3'-dideoxycytidine and thymine as substrates
0.017
-
Q9RSV5
wild-type, with 2',3'-dideoxythymidine and adenine as substrates
0.02
-
-, Q6YNI5
mutant G9S, with 2'-deoxyinosine and adenine as substrates
0.03
-
-, Q6YNI5
wild-type, with 2',3'-dideoxythymidine and adenine as substrates
0.03
-
Q6YNI5
wild-type, 2',3'-dideoxyadenosine and cytosine as substrates; wild-type, 2,3'-dideoxythymidine and adenine as substrates
0.036
-
-, Q6YNI5
wild-type, with 2',3'didehydro-2',3'-dideoxythymidine and adenine as substrates
0.036
-
Q6YNI5
wild-type, 2',3'didehydro-2',3'-dideoxythymidine and adenine as substrates
0.04
-
Q9RSV5
mutant G9S, with 2',3'-dideoxyinosine and adenine as substrates; wild-type, with 2',3'-dideoxycytidine and hypoxanthine as substrates
0.05
-
-, Q6YNI5
wild-type, with 2',3'-dideoxycytidine and hypoxanthine as substrates
0.05
-
Q6YNI5
wild-type, 2',3'-dideoxycytidine and hypoxanthine as substrates
0.056
-
Q9RSV5
wild-type, with 2',3'-dideoxyuridine and cytosine as substrates
0.06
-
-, Q6YNI5
mutant G9S, with 2',3'-dideoxyinosine and adenine as substrates; wild-type, with 2'-deoxycytidine and hypoxanthine as substrates
0.06
-
Q6YNI5
wild-type, 2',3'-dideoxycytidine and adenine as substrates
0.19
-
-, Q6YNI5
wild-type, with 2',3'-dideoxyuridine and cytosine as substrates
0.2
-
Q9RSV5
mutant G9S, with 2',3'didehydro-2',3'-dideoxythymidine and adenine as substrates
0.25
-
Q9RSV5
mutant G9S, with 3'-dideoxythymidine and adenine as substrates
0.4
-
Q9RSV5
mutant G9S, with 2',3'-dideoxyguanosine and thymine as substrates
0.42
-
Q6YNI5
mutant A15T, 2',3'-dideoxyadenosine and thymine as substrates
0.46
-
Q6YNI5
mutant A15T, 2'-deoxyadenosine and thymine as substrates
0.47
-
Q9RSV5
mutant G9S, with 2'-deoxyinosine and adenine as substrates
0.5
-
-, Q6YNI5
mutant G9S, with 3'-dideoxythymidine and adenine as substrates
0.5
-
Q6YNI5
mutant A15T, 2,3'-dideoxythymidine and adenine as substrates
0.57
-
-, Q6YNI5
mutant G9S, with 2'-deoxyguanosine and thymine
0.6
-
-, Q6YNI5
mutant G9S, with 2',3'-dideoxyguanosine and thymine as substrates
0.8
-
-
reaction with deoxyinosine and adenine
0.8
-
Q9RSV5
mutant G9S, with 2',3'-dideoxycytidine and hypoxanthine as substrates
1.04
-
Q9RSV5
mutant G9S, with 2'-deoxythymidine and adenine as substrates
1.08
-
-, Q6YNI5
wild-type, with 2'-deoxyinosine and adenine as substrates
1.1
-
Q9RSV5
mutant G9S, with 2'-deoxyguanosine and thymine
1.1
-
Q6YNI5
mutant A15T, 2'-deoxyadenosine and cytosine as substrates
1.25
-
Q9RSV5
mutant G9S, with 2'-deoxycytidine and hypoxanthine as substrates
1.29
-
-, Q6YNI5
mutant G9S, with 2',3'-dideoxycytidine and adenine as substrates
1.29
-
Q6YNI5
mutant A15T, 2'-deoxycytidine and hypoxanthine as substrates
1.3
-
Q9RSV5
mutant G9S, with 2',3'-dideoxyuridine and cytosine as substrates
1.35
-
Q6YNI5
mutant A15T, 2',3'-dideoxyadenosine and cytosine as substrates
1.47
-
Q6YNI5
mutant A15T, 2',3'-dideoxycytidine and thymine as substrates
1.6
-
-, Q6YNI5
mutant G9S, with 2',3'-dideoxyuridine and cytosine as substrates
1.9
-
-, Q6YNI5
mutant G9S, with 2'-deoxythymidine and adenine as substrates
1.9
-
Q6YNI5
mutant A15T, 2'-deoxythymidine and adenine as substrates
3.1
-
Q9RSV5
mutant G9S, with 2'-deoxyuridine and cytosine as substrates
3.6
-
Q9RSV5
wild-type, with 2'-deoxyinosine and adenine as substrates
4.95
-
-, Q6YNI5
wild-type, with 2'-deoxyguanosine and thymine
5.2
-
Q9RSV5
wild-type, with 2'-deoxyguanosine and thymine
6
-
Q9RSV5
mutant G9S, with 2',3'-dideoxycytidine and adenine as substrates
6.67
-
-
equals 100%, purified protein, in presence of 0.5 mg/ml bovine serum albumin, pH 7.5, 10 min, 25C
6.8
-
-, Q6YNI5
mutant G9S, with 2',3'-dideoxycytidine and hypoxanthine as substrates
6.8
-
Q6YNI5
mutant A15T, 2',3'-dideoxycytidine and hypoxanthine as substrates
6.9
-
-, Q6YNI5
wild-type, with 2',3'-dideoxycytidine and adenine as substrates
6.9
-
Q6YNI5
wild-type, 2'-deoxycytidine and hypoxanthine as substrates
7.1
-
-, Q6YNI5
mutant G9S, with 2'-deoxycytidine and hypoxanthine as substrates
7.1
-
Q6YNI5
mutant A15T, 2',3'-dideoxycytidine and adenine as substrates
7.11
-
Q6YNI5
mutant A15T, 2'-deoxycytidine and thymine as substrates
7.75
-
-
reaction with thymidine and cytosine
8.4
-
Q6YNI5
wild-type, 2'-deoxyadenosine and thymine as substrates
9.28
-
-, Q6YNI5
mutant G9S, with 2',3'didehydro-2',3'-dideoxythymidine and adenine as substrates
9.28
-
Q6YNI5
mutant A15T, 2',3'didehydro-2',3'-dideoxythymidine and adenine as substrates
9.3
-
-, Q6YNI5
mutant G9S, with 2'-deoxycytidine and adenine as substrates
9.3
-
Q6YNI5
mutant A15T, 2'-deoxycytidine and adenine as substrates
11.9
-
Q9RSV5
wild-type, with 2'-deoxythymidine and adenine as substrates
12.7
-
-, Q6YNI5
mutant G9S, with 2'-deoxyuridine and cytosine as substrates
20.1
-
-, Q6YNI5
wild-type, with 2'-deoxythymidine and adenine as substrates
20.1
-
Q6YNI5
wild-type, 2'-deoxythymidine and adenine as substrates
27.1
-
Q9RSV5
mutant G9S, with 2'-deoxycytidine and adenine as substrates
29
-
Q9RSV5
wild-type, with 2',3'-dideoxycytidine and adenine as substrates
32
-
Q6YNI5
wild-type, 2'-deoxycytidine and thymine as substrates
33.2
-
Q6YNI5
wild-type, 2'-deoxyadenosine and cytosine as substrates
36.8
-
-
DRTase II, reaction with thymidine + adenine
40.2
-
-, Q6YNI5
wild-type, with 2'-deoxyuridine and cytosine as substrates
42.5
-
Q9RSV5
wild-type, with 2'-deoxyuridine and cytosine as substrates
42.9
-
-
DRTase I, reaction with deoxyinosine and adenine
43
-
-
reaction with deoxycytidine and adenine
83.7
-
-, Q6YNI5
wild-type, with 2'-deoxycytidine and adenine as substrates
83.7
-
Q6YNI5
wild-type, 2'-deoxycytidine and adenine as substrates
173
-
Q9RSV5
wild-type, with 2'-deoxycytidine and adenine as substrates
additional information
-
-
method for determining enzyme activity by coupling the release of adenine from deoxyadenosine to the reaction catalyzed by pyruvate kinase/lactate dehydrogenase via adenine phosphoribosyltransferase and adenylate kinase
additional information
-
-
0.9% in presence of 1 mM ZnCl2; 102% in presence of 1 mM NaCl; 105% in presence of 1 mM CsCl; 106% in presence of 1 mM NaSO4; 12.9% in presence of 1 mM CoCl2; 143% in presence of 1 mM NH4Cl; 151% in presence of 1 mM RbCl; 163% in presence of 1 mM (NH4)2SO4; 165% in presence of 1 mM K2SO4; 166% in presence of 1 mM KCl; 166% in presence of 1 mM (NH4)2SO4 and 1 mM KCl; 192% in presence of 10 mM KCl; 194% in presence of 10 mM (NH4)2SO4; 3.2% in presence of 1 mM CuSO4; 51.8% in presence of 1 mM CaCl2; 66.3% in presence of 1 mM MgCl2; 66% in presence of 1 mM MgSO4; 68.3% in presence of 1 mM FeCl2; 97.7% in presence of 1 mM LiCl
pH OPTIMUM
pH MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
6.5
-
-
assay at
7.5
-
-
assay at
7.7
-
-
reaction with deoxyinosine and guanine
pH RANGE
pH RANGE MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
5
8
-
50% of maximal activity at pH 7.0 with substrate dCMP, 20% with dGMP, 10% of maximal activity at pH 8.0 with both substrates
TEMPERATURE OPTIMUM
TEMPERATURE OPTIMUM MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
30
-
-
assay at, pH 7.5
37
-
-
assay at
40
-
-
assay at
SOURCE TISSUE
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
SOURCE
LOCALIZATION
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
GeneOntology No.
LITERATURE
SOURCE
-
attached to cell wall surface, enzyme can be released into the culture broth
Manually annotated by BRENDA team
Lactobacillus fermentum CGMCC 1.2133
-
attached to cell wall surface, enzyme can be released into the culture broth
-
Manually annotated by BRENDA team
Lactobacillus fermentum CGMCC 1.2133
-
-
-
Manually annotated by BRENDA team
MOLECULAR WEIGHT
MOLECULAR WEIGHT MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
18080
-
Q9RSV5
native enzyme, mass spectrometry
18110
-
Q9RSV5
mutant, mass spectrometry
18290
-
-
theoretical molecular weight
18900
-
-, Q6YNI5
native enzyme, mass spectrometry
18930
-
-, Q6YNI5
mutant, mass spectrometry
20440
-
-
monomeric enzyme, equilibrium sedimentation ultracentrifugation
25500
-
-
according to migration in SDS-12.5% polyacrylamide gel electrophoresis
40520
-
-
monomeric enzyme, equilibrium sedimentation ultracentrifugation
69000
-
-
gel filtration chromatography
82000
-
-
gel filtration
110000
-
-
gel filtration
110000
-
-
DRTase II, non-denaturing PAGE
112700
-
-
sedimentation equilibrium experiments
120000
-
-
DRTase I, nondenaturing PAGE
SUBUNITS
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
?
-
x * 17000, SDS-PAGE
?
-
x * 18000, SDS-PAGE
?
Q8RLY5
x * 24000, SDS-PAGE
?
-
x * 18000, SDS-PAGE, x * 18280, calculated
?
Lactobacillus fermentum CGMCC 1.2133
-
x * 18000, SDS-PAGE, x * 18280, calculated
-
dimer
-
2 * 17649, unmodified recombinant enzyme, mass spectrometry, 2 * 17649, unmodified recombinant enzyme, mass spectrometry, 2 * 20440, His-tagged recombinant enzyme, SDS-PAGE
hexamer
-
6 * 18000, DRTase II, SDS-PAGE; 6 * 20000, DRTAse I
hexamer
-
6 * 18000, SDS-PAGE
homotetramer
-
4 * 17250, elution as single peak from the preparative gel filtration column
monomer
-
1 * 17649, unmodified recombinant enzyme, mass spectrometry, 1 * 19812, His-tagged recombinant enzyme, mass spectrometry, 1 * 20440, His-tagged recombinant enzyme, SDS-PAGE
additional information
-
at 0.003 mM the His-RCL is mostly monomeric, at 0.03 mM, His-RCL is mostly dimeric, at 0.01 mM His-RCL is at equilibrium between monomeric and dimeric states, overview
additional information
-
ligand binding and active site structures, overview
Crystallization/COMMENTARY
ORGANISM
UNIPROT ACCESSION NO.
LITERATURE
microdiffusion by means of equilibrium dialysis is used to crystallize the enzyme
-
purified recombinant selenomethionine-labeled enzyme free or in complex with benzo[cd]indol-2(1H)-one, 5-aminoisoquinoline, 2-ethylbenzyl alcohol, or 1-methylquinoline-2(1H)-one, fragment cocktail crystallography, X-ray diffraction structure determination and analysis at 1.5-1.8 A resolution, SAD X-ray techniques
-
pH STABILITY
pH STABILITY MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
4
8
-
enzyme is completely stable in this pH interval
4
-
-
61C, 30 min, about 30% loss of activity
6.5
-
-
61C, 30 min, maximal stability
6.5
-
-
optimal stability
8
-
-
61C, 30 min, about 15% loss of activity
8.5
-
-
61C, 30 min, about 70% of maximal activity
TEMPERATURE STABILITY
TEMPERATURE STABILITY MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
25
-
-
pH 7.5, 20 min, 60% activity lost, in presence of 10 mM (NH4)2SO4 only 20% activity lost, in presence of 500 mM KCL only 6% activity lost
30
-
-
pH 7.5, 20 min, in presence of 500 mM (NH4)2SO4 fully active
35
-
-
and more, pH 7.5, 20 min, 75% activity lost
50
-
-
stable up to
55
-
-
pH 6.3, 10 min, stable below
64
-
-
melting temperature
90
-
-
pH 6.3, 10 min, 75% loss of activity
additional information
-
-
(NH4)2SO4 is more thermostabilizing than KCl
GENERAL STABILITY
ORGANISM
UNIPROT ACCESSION NO.
LITERATURE
crystals are stable to X-rays for at least 5 days
-
ORGANIC SOLVENT
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
2-propanol
-
enzyme immobilized on Sepabeads shows 1.2-1.4fold enhanced activity at 20% of methanol, ethanol, 2-propanol, ethylene glycol, and acetone, and at 10% and 30% acetonitrile. Highest increase in activity is obtained in presence of 20% acetonitrile
Acetone
-
enzyme immobilized on Sepabeads shows 1.2-1.4fold enhanced activity at 20% of methanol, ethanol, 2-propanol, ethylene glycol, and acetone, and at 10% and 30% acetonitrile. Highest increase in activity is obtained in presence of 20% acetonitrile
acetonitrile
-
enzyme immobilized on Sepabeads shows 1.2-1.4fold enhanced activity at 20% of methanol, ethanol, 2-propanol, ethylene glycol, and acetone, and at 10% and 30% acetonitrile. Highest increase in activity is obtained in presence of 20% acetonitrile
Ethanol
-
enzyme immobilized on Sepabeads shows 1.2-1.4fold enhanced activity at 20% of methanol, ethanol, 2-propanol, ethylene glycol, and acetone, and at 10% and 30% acetonitrile. Highest increase in activity is obtained in presence of 20% acetonitrile
Ethylene glycol
-
enzyme immobilized on Sepabeads shows 1.2-1.4fold enhanced activity at 20% of methanol, ethanol, 2-propanol, ethylene glycol, and acetone, and at 10% and 30% acetonitrile. Highest increase in activity is obtained in presence of 20% acetonitrile
Methanol
-
enzyme immobilized on Sepabeads shows 1.2-1.4fold enhanced activity at 20% of methanol, ethanol, 2-propanol, ethylene glycol, and acetone, and at 10% and 30% acetonitrile. Highest increase in activity is obtained in presence of 20% acetonitrile
STORAGE STABILITY
ORGANISM
UNIPROT ACCESSION NO.
LITERATURE
-50C, stable for several months either in the lyophilized form or disolved in freshly distilled water
-
-20C, purified recombinant enzyme, 1 mg/ml, at least 1 year without loss of activity
-
Purification/COMMENTARY
ORGANISM
UNIPROT ACCESSION NO.
LITERATURE
DRTase I and DRTAse II
-
preclearing of bacterial lysate with 40% ammonium sulfate and subsequent precipitation of proteins with 90% ammonium sulfate, gel filtration of dissolved protein on Sephacryl S-200 column, combination of fractions with N-deoxyribosyltransferase activity, precipitation of proteins with 90% ammonium sulfate, dialysis of dissolved proteins and concentration by ultrafiltration, second gel filtration on Sephacryl S-200 column, combination of active fractions and concentration by ultrafiltration, recovery of 70.4% of activity, storage at -20C in 50% glycerol
-
recombinant unmodified enzyme from Escherichia coli strain Bli5 (BL21 (DE3) pDIA17) by anion exchange chromatography and gel filtration, recombinant His-tagged enzyme by nickel affinity chromatography, over 95% purity
-
recombinant His-tagged wild-type and selenomethionine-labeled enzymes from Escherichia coli to homogeneity by nickel affinity chromatography and gel filtration
-
Cloned/COMMENTARY
ORGANISM
UNIPROT ACCESSION NO.
LITERATURE
expression in Escherichia coli
Q8RLY5
expression of the catalytically inactive mutant E98A in Escherichia coli
-
expression in Escherichia coli
-
in pET-22b(+) for expression in Escherichia coli BL21(DE3); in pGEM-T for cloning
-
gene Rc1, expression of soluble unmodified enzyme and His-tagged enzymes in Escherichia coli strain Bli5 (BL21 (DE3) pDIA17)
-
expression of His-tagged wild-type and selenomethionine-labeled enzymes in Escherichia coli
-
ENGINEERING
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
A15C
-, Q6YNI5
kcat is diminished as compared to wild-type
A15C
Q6YNI5
is not capable of improving 2',3'-dideoxyribose transfer reaction
A15S
-, Q6YNI5
activity with 2'-deoxycytidine and adenine as substrates is closer to that of the wild-type enzyme than that of the A15T mutant, kcat is diminished as compared to wild-type
A15S
Q6YNI5
Ser appears a good alternative for Thr, rate of 2',3'-dideoxyribosyl transfer from 2',3'-dideoxycytidine to adenine of the two mutants is almost identical
A15T
-, Q6YNI5
mutation allows conversion of the nucleoside 2'deoxyribosyltransferase activity to a nucleoside 2',3'-dideoxy- or 2',3'-didehydro,2',3'-dideoxyribosyltransferase activity, kcat is diminished as compared to wild-type
A15T
Q6YNI5
a single amino-acid substitution allows the conversion of the nucleoside 2'-deoxyribosyltransferase activity to a nucleoside 2',3'-dideoxy- or 2'3'-didehydro, 2',3'-dideoxyribosyltransferase activity. Specificity for the base is unchanged compared to the wild-type. The mutant displays an improved activity to the transfer of 2',3'-dideoxyribose, which is enhanced by 250fold (depending on the donors and acceptors) while the deoxyribosyl group transfer remains acceptable (30% of the wild-type)
A15V
-, Q6YNI5
can not improve 2',3'-dideoxyribose transfer reaction, kcat is diminished as compared to wild-type
A15C
Lactobacillus fermentum PAK9
-
kcat is diminished as compared to wild-type
-
A15S
Lactobacillus fermentum PAK9
-
activity with 2'-deoxycytidine and adenine as substrates is closer to that of the wild-type enzyme than that of the A15T mutant, kcat is diminished as compared to wild-type
-
A15T
Lactobacillus fermentum PAK9
-
mutation allows conversion of the nucleoside 2'deoxyribosyltransferase activity to a nucleoside 2',3'-dideoxy- or 2',3'-didehydro,2',3'-dideoxyribosyltransferase activity, kcat is diminished as compared to wild-type
-
A15V
Lactobacillus fermentum PAK9
-
can not improve 2',3'-dideoxyribose transfer reaction, kcat is diminished as compared to wild-type
-
E98A
-
catalytically inactive mutant
G9C
Q9RSV5
can not improve 2',3'-dideoxyribose transfer reaction, kcat is diminished as compared to wild-type
G9S
Q9RSV5
mutation allows conversion of the nucleoside 2'deoxyribosyltransferase activity to a nucleoside 2',3'-dideoxy- or 2',3'-didehydro,2',3'-dideoxyribosyltransferase activity, kcat is diminished as compared to wild-type
G9S
-
a single amino-acid substitution allows the conversion of the nucleoside 2'-deoxyribosyltransferase activity to a nucleoside 2',3'-dideoxy- or 2'3'-didehydro, 2',3'-dideoxyribosyltransferase activity
G9T
Q9RSV5
Ser remains a better substituent for Gly-9 than Thr, kcat is diminished as compared to wild-type
E105A
-
site-directed mutagenesis, the mutant shows about 200fold reduced activity and a 170fold increased Km compared to the wild-type enzyme
additional information
-
immobilization on calcium alginate or calcium pectate of the enzyme increases the 2'-deoxynucleoside synthesis effiency of the organism, method optimization, overview
additional information
Bacillus coagulans CECT 12
-
immobilization on calcium alginate or calcium pectate of the enzyme increases the 2'-deoxynucleoside synthesis effiency of the organism, method optimization, overview
-
additional information
-
immobilization on calcium alginate or calcium pectate of the enzyme increases the 2'-deoxynucleoside synthesis effiency of the organism, method optimization, overview
additional information
Bacillus psychrosaccharolyticus CECT 4074
-
immobilization on calcium alginate or calcium pectate of the enzyme increases the 2'-deoxynucleoside synthesis effiency of the organism, method optimization, overview
-
G9W
Q9RSV5
can not improve 2',3'-dideoxyribose transfer reaction, kcat is diminished as compared to wild-type
additional information
-
immobilization on calcium alginate or calcium pectate of the enzyme increases the 2'-deoxynucleoside synthesis effiency of the organism, method optimization, overview
additional information
Lactobacillus sp. CECT 4219
-
immobilization on calcium alginate or calcium pectate of the enzyme increases the 2'-deoxynucleoside synthesis effiency of the organism, method optimization, overview
-
additional information
-
immobilization on calcium alginate or calcium pectate of the enzyme increases the 2'-deoxynucleoside synthesis effiency of the organism, method optimization, overview
additional information
Psychrobacter immobilis CECT 4492
-
immobilization on calcium alginate or calcium pectate of the enzyme increases the 2'-deoxynucleoside synthesis effiency of the organism, method optimization, overview
-
APPLICATION
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
synthesis
-
useful as biocatalyst, immobilization on calcium alginate or calcium pectate of the enzyme increases the 2'-deoxynucleoside synthesis effiency of the organism
synthesis
Bacillus coagulans CECT 12
-
useful as biocatalyst, immobilization on calcium alginate or calcium pectate of the enzyme increases the 2'-deoxynucleoside synthesis effiency of the organism
-
synthesis
-
useful as biocatalyst, immobilization on calcium alginate or calcium pectate of the enzyme increases the 2'-deoxynucleoside synthesis effiency of the organism
synthesis
Bacillus psychrosaccharolyticus CECT 4074
-
useful as biocatalyst, immobilization on calcium alginate or calcium pectate of the enzyme increases the 2'-deoxynucleoside synthesis effiency of the organism
-
synthesis
-
development of a practical method for enzymatic synthesis of deoxyguanosine by the combination of transglycosylation with NdRT-II from thymidine to a 2-amino-6-substituted purine, and the hydrolysis reaction with bacterial adenosine deaminase
synthesis
-
covalent attachment of recombinant Lactobacillus reuteri 2'-deoxyribosyltransferase to Sepabeads EC-EP303. The immobilized enzyme retains 50% of its maximal activity after 17.9 h at 60C, and 96% activity is observed after storage at 40C for 110 h. Immobilized enzyme can be recycled for 26 consecutive batch reactions in the synthesis of 2,6-diaminopurine-2'-deoxyriboside with negligible loss of catalytic activity and can be employed in the enzymatic synthesis of different natural and therapeutic nucleosides such as 5-ethyl-2'-deoxyuridine and 5-trifluorothymidine
synthesis
-
immobilization of 2'-deoxyribosyltransferase from Lactobacillus reuteri on Sepabeads. Immobilized enzyme activity is enhanced 1.21.4fold at 20% of methanol, ethanol, 2-propanol, diethylene glycol, and acetone, and at 10% and 30% acetonitrile. Highest increased activity is also obtained in presence of 20% acetonitrile. Immobilized enzyme is successfully used in the synthesis of 2'-deoxyxanthosine and 2'-deoxyguanosine using 2'-deoxyuridine as sugar donor and the corresponding poor water-soluble base in the presence of 30% of methanol, ethanol, 2-propanol, ethylene glycol, acetonitrile, and DMSO, giving high nucleoside yields at 4 h
medicine
-
the low substrate specificity of the enzyme is used advantageously for synthesis of nucleoside analogs, some of them of medical interest
synthesis
-
useful as biocatalyst, immobilization on calcium alginate or calcium pectate of the enzyme increases the 2'-deoxynucleoside synthesis effiency of the organism
synthesis
Lactobacillus sp. CECT 4219
-
useful as biocatalyst, immobilization on calcium alginate or calcium pectate of the enzyme increases the 2'-deoxynucleoside synthesis effiency of the organism
-
synthesis
-
useful as biocatalyst, immobilization on calcium alginate or calcium pectate of the enzyme increases the 2'-deoxynucleoside synthesis effiency of the organism
synthesis
Psychrobacter immobilis CECT 4492
-
useful as biocatalyst, immobilization on calcium alginate or calcium pectate of the enzyme increases the 2'-deoxynucleoside synthesis effiency of the organism
-