Information on EC 2.4.2.6 - nucleoside deoxyribosyltransferase

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The expected taxonomic range for this enzyme is: Bacteria, Eukaryota

EC NUMBER
COMMENTARY hide
2.4.2.6
-
RECOMMENDED NAME
GeneOntology No.
nucleoside deoxyribosyltransferase
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
2-deoxy-D-ribosyl-base1 + base2 = 2-deoxy-D-ribosyl-base2 + base1
show the reaction diagram
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
pentosyl group transfer
PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
Pyrimidine metabolism
-
-
SYSTEMATIC NAME
IUBMB Comments
nucleoside:purine(pyrimidine) deoxy-D-ribosyltransferase
Base1 and base2 represent various purines and pyrimidines.
CAS REGISTRY NUMBER
COMMENTARY hide
9026-86-2
-
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
strain CECT 12
-
-
Manually annotated by BRENDA team
strain CECT 12
-
-
Manually annotated by BRENDA team
strain CECT 4074
-
-
Manually annotated by BRENDA team
strain CECT 4074
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
strain PAK9
UniProt
Manually annotated by BRENDA team
strain CECT 4219
-
-
Manually annotated by BRENDA team
subsp. lactis JCM5808
-
-
Manually annotated by BRENDA team
strain CECT 4492
-
-
Manually annotated by BRENDA team
strain CECT 4492
-
-
Manually annotated by BRENDA team
gene Rc1
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
2',3'-dideoxyadenosine + cytosine
2',3'-dideoxycytidine + adenine
show the reaction diagram
-
-
-
?
2',3'-dideoxyadenosine + thymine
2',3'-dideoxythymidine + adenine
show the reaction diagram
-
-
-
?
2',3'-dideoxycytidine + adenine
2',3'-dideoxyadenosine + cytosine
show the reaction diagram
2',3'-dideoxycytidine + hypoxanthine
2',3'-dideoxyinosine + cytosine
show the reaction diagram
2',3'-dideoxycytidine + thymine
2',3'-dideoxythymidine + cytosine
show the reaction diagram
-
-
-
?
2',3'-dideoxyguanosine + thymine
2',3'-dideoxythymidine + guanine
show the reaction diagram
2',3'-dideoxyinosine + adenine
2',3'-dideoxyadenosine + hypoxanthine
show the reaction diagram
2',3'-dideoxythymidine + adenine
2',3'-dideoxyadenosine + thymine
show the reaction diagram
2',3'-dideoxyuridine + cytosine
2',3'-dideoxycytidine + uracil
show the reaction diagram
2',3'didehydro-2',3'-dideoxythymidine + adenine
2',3'didehydro-2',3'-dideoxyadenosine + thymine
show the reaction diagram
2'-deoxy-D-ribosyl-uracil + adenine
2'-deoxy-D-ribosyl-adenine + uracil
show the reaction diagram
2'-deoxyadenosine + 5-chlorouracil
2'-deoxy-D-ribosyl-5-chlorouracil + adenine
show the reaction diagram
2'-deoxyadenosine + 5-fluorouracil
2'-deoxy-5-fluorouridine + adenine
show the reaction diagram
2'-deoxyadenosine + 5-fluorouracil
2'-deoxy-D-ribosyl-5-fluorouracil + adenine
show the reaction diagram
reaction is catalyzed by mutants F13R/D92S/N123S and F13Q/D92S/N123S
-
-
?
2'-deoxyadenosine + cytosine
2'-deoxycytidine + adenine
show the reaction diagram
2'-deoxyadenosine + thymine
2'-deoxythymidine + adenine
show the reaction diagram
-
-
-
?
2'-deoxycytidine + 2,6-diaminopurine
2,6-diaminopurine 2-deoxy-D-ribose 5-phosphate + cytosine
show the reaction diagram
reaction is catalyzed by mutants F13R/D92S/N123S and F13Q/D92S/N123S
-
-
?
2'-deoxycytidine + 2,6-dichloropurine
2,6-dichloropurine 2-deoxy-D-ribose 5-phosphate + cytosine
show the reaction diagram
reaction is catalyzed by mutants F13R/D92S/N123S and F13Q/D92S/N123S
-
-
?
2'-deoxycytidine + 5-bromouracil
2'-deoxy-5-bromouridine + cytosine
show the reaction diagram
reaction is catalyzed by mutants F13R/D92S/N123S and F13Q/D92S/N123S
-
-
?
2'-deoxycytidine + 6-chloropurine
6-chloropurine 2-deoxy-D-ribose 5-phosphate + cytosine
show the reaction diagram
reaction is catalyzed by mutants F13R/D92S/N123S and F13Q/D92S/N123S
-
-
?
2'-deoxycytidine + adenine
2'-deoxyadenosine + cytosine
show the reaction diagram
2'-deoxycytidine + hypoxanthine
2'-deoxyinosine + cytosine
show the reaction diagram
2'-deoxycytidine + thymine
2'-deoxythymidine + cytosine
show the reaction diagram
-
-
-
?
2'-deoxyguanosine + thymine
2'-deoxythymidine + guanine
show the reaction diagram
2'-deoxyinosine + adenine
2'-deoxyadenosine + hypoxanthine
show the reaction diagram
2'-deoxyinosine + uracil
2'-deoxyuridine + hypoxanthine
show the reaction diagram
-
-
-
-
?
2'-deoxythymidine + adenine
2'-deoxyadenosine + thymine
show the reaction diagram
2'-deoxyuridine + 2,6-diaminopurine
2,6-diaminopurine 2-deoxy-D-ribose 5-phosphate + uracil
show the reaction diagram
2'-deoxyuridine + 5-azacytosine
5-aza-2'-deoxycytidine + uracil
show the reaction diagram
-
-
-
-
?
2'-deoxyuridine + 5-ethyluracil
5-ethyl-2'-deoxyuridine + uracil
show the reaction diagram
-
-
-
-
?
2'-deoxyuridine + 5-fluorocytosine
2'-deoxy-5-fluorocytidine + uracil
show the reaction diagram
2'-deoxyuridine + 5-methylcytosine
5-methyl-2'-deoxycytidine + uracil
show the reaction diagram
-
-
-
-
?
2'-deoxyuridine + 6-mercaptopurine
6-mercaptopurine 2-deoxy-D-ribose 5-phosphate + uracil
show the reaction diagram
2'-deoxyuridine + adenine
2'-deoxyadenosine + uracil
show the reaction diagram
-
-
-
-
?
2'-deoxyuridine + benzimidazole
benzimidazole 2-deoxy-D-ribose 5-phosphate + uracil
show the reaction diagram
2'-deoxyuridine + cytosine
2'-deoxycytidine + uracil
show the reaction diagram
2'-deoxyuridine + hypoxanthine
2'-deoxyinosine + uracil
show the reaction diagram
-
-
-
-
?
5'-fluoro-5'-thymidine + adenine
5'-fluoro-5'-deoxythymine + adenosine
show the reaction diagram
-
deoxyribosyltransferase II
-
-
?
adenine + deoxycytidine
deoxyadenosine + cytosine
show the reaction diagram
-
64.0% conversion after 4 h at standard reaction conditions: 2 mM each substrate, 0.013 U NTD, pH 7.5, 25C, 40 mM (NH4)2SO4, 0.5 mg/ml bovine serum albumin
-
-
r
adenine + deoxyguanosine
deoxyadenosine + guanine
show the reaction diagram
-
46.6% conversion after 4 h at standard reaction conditions: 2 mM each substrate, 0.013 U NTD, pH 7.5, 25C, 40 mM (NH4)2SO4, 0.5 mg/ml bovine serum albumin
-
-
r
adenine + deoxyinosine
deoxyadenosine + hypoxanthine
show the reaction diagram
-
57.3% conversion after 4 h at standard reaction conditions: 2 mM each substrate, 0.013 U NTD, pH 7.5, 25C, 40 mM (NH4)2SO4, 0.5 mg/ml bovine serum albumin
-
-
r
adenine + deoxyuridine
deoxyadenosine + uracil
show the reaction diagram
-
75.4% conversion after 4 h at standard reaction conditions: 2 mM each substrate, 0.013 U NTD, pH 7.5, 25C, 40 mM (NH4)2SO4, 0.5 mg/ml bovine serum albumin
-
-
r
adenine + thymidine
deoxyadenosine + thymine
show the reaction diagram
-
74.6% conversion after 4 h at standard reaction conditions: 2 mM each substrate, 0.013 U NTD, pH 7.5, 25C, 40 mM (NH4)2SO4, 0.5 mg/ml bovine serum albumin
-
-
r
cytosine + deoxyadenosine
deoxycytidine + adenine
show the reaction diagram
-
27.7% conversion after 4 h at standard reaction conditions: 2 mM each substrate, 0.013 U NTD, pH 7.5, 25C, 40 mM (NH4)2SO4, 0.5 mg/ml bovine serum albumin
-
-
r
cytosine + deoxyguanosine
deoxycytidine + guanine
show the reaction diagram
-
25.7% conversion after 4 h at standard reaction conditions: 2 mM each substrate, 0.013 U NTD, pH 7.5, 25C, 40 mM (NH4)2SO4, 0.5 mg/ml bovine serum albumin
-
-
r
cytosine + deoxyinosine
deoxycytidine + hypoxanthine
show the reaction diagram
-
32.0% conversion after 4 h at standard reaction conditions: 2 mM each substrate, 0.013 U NTD, pH 7.5, 25C, 40 mM (NH4)2SO4, 0.5 mg/ml bovine serum albumin
-
-
r
cytosine + deoxyuridine
deoxycytidine + uracil
show the reaction diagram
-
62.9% conversion after 4 h at standard reaction conditions: 2 mM each substrate, 0.013 U NTD, pH 7.5, 25C, 40 mM (NH4)2SO4, 0.5 mg/ml bovine serum albumin
-
-
r
cytosine + thymidine
deoxycytidine + thymine
show the reaction diagram
dAMP + base
dNMP + adenine
show the reaction diagram
-
-
-
-
r
dCMP + base
dNMP + cytidine
show the reaction diagram
-
-
-
-
r
deoxyadenosine + 2,6-dichloropurine
adenine + 9-(2-deoxy-beta-D-ribofuranosyl)-2,6-dichloropurine
show the reaction diagram
-
-
-
-
?
deoxyadenosine + 2-amino 6-methylmercaptopurine
adenine + 9-(2-deoxy-beta-D-ribofuranosyl)-2-amino-6-methylmercaptopurine
show the reaction diagram
-
-
-
-
?
deoxyadenosine + 2-amino-6-hydroxy 8-mercaptopurine
adenine + ?
show the reaction diagram
-
-
-
-
?
deoxyadenosine + 2-aminobenzimidazole
adenine + ?
show the reaction diagram
-
-
-
-
?
deoxyadenosine + 2-hydroxybenzimidazole
adenine + ?
show the reaction diagram
-
-
-
-
?
deoxyadenosine + 2-thiouracil
adenine + deoxy-2-thiouridine
show the reaction diagram
-
-
-
-
?
deoxyadenosine + 4-amino-5-imidazole carboxamide
adenine + ?
show the reaction diagram
-
-
-
-
?
deoxyadenosine + 5-fluorouracil
adenine + deoxy-5-fluorouridine
show the reaction diagram
-
-
-
-
?
deoxyadenosine + 5-iodouracil
adenine + deoxy-5-iodouridine
show the reaction diagram
-
-
-
-
?
deoxyadenosine + 6-azathymine
adenine + deoxy-6-deazathymidine
show the reaction diagram
-
-
-
-
?
deoxyadenosine + 6-chloroguanine
adenine + 2'-deoxy-6-chloroguanosine
show the reaction diagram
-
-
-
-
?
deoxyadenosine + 6-chloropurine
adenine + 9-(2-deoxy-beta-D-ribofuranosyl)-6-chloropurine
show the reaction diagram
-
-
-
-
?
deoxyadenosine + 6-dimethylaminopurine
adenine + 9-(2-deoxy-beta-D-ribofuranosyl)-6-dimethylaminopurine
show the reaction diagram
-
-
-
-
?
deoxyadenosine + 6-methylpurine
adenine + 9-(2-deoxy-beta-D-ribofuranosyl)-6-methylpurine
show the reaction diagram
-
-
-
-
?
deoxyadenosine + adenine
adenine + deoxyadenosine
show the reaction diagram
-
-
-
-
?
deoxyadenosine + cytosine
adenine + deoxycytidine
show the reaction diagram
deoxyadenosine + ethyl 4-amino-1H-imidazole-5-carboxylate
adenine + ethyl 4-amino-1-(2-deoxy-b-D-erythro-pentofuranosyl)-1H-imidazole-5-carboxylate
show the reaction diagram
-
-
-
-
?
deoxyadenosine + hypoxanthine
adenine + deoxyinosine + 7-(beta-D-2'-deoxyribufuranosyl)hypoxanthine
show the reaction diagram
deoxyadenosine + thymine
adenine + thymidine
show the reaction diagram
-
-
-
-
r
deoxyadenosine + uracil
adenine + deoxyuridine
show the reaction diagram
-
-
-
-
r
deoxyadenosine + xanthine
adenine + deoxyxanthosine
show the reaction diagram
-
-
-
-
r
deoxycytidine + 1,7-dimethylguanine
cytosine + deoxy-1,7-dimethylguanosine
show the reaction diagram
-
DRTase II
-
-
?
deoxycytidine + 1,N2-epsilon-guanine
cytosine + ?
show the reaction diagram
-
deoxyribosylation at the N9 atom
-
-
?
deoxycytidine + 1,N6-epsilon-adenine
cytosine + ?
show the reaction diagram
-
deoxyribosylation at the N9 atom
-
-
?
deoxycytidine + 3,N4-epsilon-cytosine
cytosine + ?
show the reaction diagram
-
deoxyribosylation at the N9 atom
-
-
?
deoxycytidine + 5,6,7,9-tetrahydro-7-acetoxy-9-oxoimidazo[1,2-a]purine
cytosine + ?
show the reaction diagram
-
deoxyribosylation at the N9 atom
-
-
?
deoxycytidine + 6-chloroguanine
hypoxanthine + deoxy-6-chloroguanosine
show the reaction diagram
-
DRTase II
-
-
?
deoxycytidine + adenine
cytosine + deoxyadenosine
show the reaction diagram
deoxycytidine + C8-aminoguanine
cytosine + 2'-deoxy-8-aminoguanosine
show the reaction diagram
-
deoxyribosylation at the N9 atom
-
-
?
deoxycytidine + C8-methylguanine
cytosine + 2'-deoxy-8-methylguanosine
show the reaction diagram
-
deoxyribosylation at the N9 atom
-
-
?
deoxycytidine + cytosine
cytosine + deoxycytidine
show the reaction diagram
-
-
-
-
?
deoxycytidine + guanine
cytosine + deoxyguanosine
show the reaction diagram
deoxycytidine + hypoxanthine
cytosine + deoxyinosine
show the reaction diagram
-
-
-
-
r
deoxycytidine + N2,3-epsilon-guanine
cytosine + ?
show the reaction diagram
-
deoxyribosylation at the N9 atom
-
-
?
deoxycytidine + N2-(2-oxoethyl)guanine
cytosine + 2'-deoxy-N2-(2-oxoethyl)guanosine
show the reaction diagram
-
deoxyribosylation at the N9 atom
-
-
?
deoxycytidine + pyrimidopurin-10-one
cytosine + ?
show the reaction diagram
-
deoxyribosylation at the N9 atom
-
-
?
deoxycytidine + thymine
cytosine + thymidine
show the reaction diagram
-
-
-
-
r
deoxycytidine + xanthine
cytosine + xanthosine
show the reaction diagram
-
-
-
-
r
deoxyguanosine + adenine
guanine + deoxyadenosine
show the reaction diagram
deoxyguanosine + cytosine
guanine + deoxycytidine
show the reaction diagram
deoxyguanosine + hypoxanthine
guanine + deoxyinosine
show the reaction diagram
-
-
-
-
r
deoxyguanosine + thymine
guanine + thymidine
show the reaction diagram
-
-
-
-
r
deoxyguanosine + uracil
guanine + deoxyuridine
show the reaction diagram
-
-
-
-
r
deoxyguanosine + xanthine
guanine + deoxyxanthine
show the reaction diagram
-
-
-
-
r
deoxyinosine + 1,7-dimethylguanine
hypoxanthine + deoxy-1,7-dimethylguanosine
show the reaction diagram
-
DRTase I
-
-
?
deoxyinosine + 6-chloroguanine
hypoxanthine + deoxy-6-chloroguanosine
show the reaction diagram
-
DRTase I
-
-
?
deoxyinosine + 8-azaguanine
hypoxanthine + deoxy-8-azaguanosine
show the reaction diagram
-
-
-
-
r
deoxyinosine + 8-azaxanthine
hypoxanthine + deoxy-8-azaxanthosine
show the reaction diagram
-
-
-
-
r
deoxyinosine + adenine
hypoxanthine + deoxyadenosine
show the reaction diagram
deoxyinosine + cytosine
hypoxanthine + deoxycytidine
show the reaction diagram
deoxyinosine + guanine
hypoxanthine + deoxyguanosine
show the reaction diagram
deoxyinosine + thymine
hypoxanthine + thymidine
show the reaction diagram
deoxyinosine + uracil
hypoxanthine + deoxyuridine
show the reaction diagram
-
-
-
-
r
deoxyinosine + uric acid
hypoxanthine + 9-(2-deoxy-beta-D-ribofuranosyl)-7,9-dihydro-3H-purine-2,6,8-trione
show the reaction diagram
-
-
-
-
r
deoxyuridine + adenine
deoxyadenosine + uracil
show the reaction diagram
-
-
-
-
?
deoxyuridine + guanine
deoxyguanosine + uracil
show the reaction diagram
-
-
-
-
?
deoxyuridine + hypoxanthine
deoxyinosine + uracil
show the reaction diagram
-
-
-
-
?
dGMP + base
dNMP + guanine
show the reaction diagram
-
dGMP is the best substrate
-
-
r
dIMP + base
dNMP + hypoxanthine
show the reaction diagram
-
-
-
-
r
dTMP + base
dNMP + thymine
show the reaction diagram
-
-
-
-
r
dUMP + base
dNMP + uracil
show the reaction diagram
-
-
-
-
r
hypoxanthine + deoxyadenosine
deoxyinosine + adenine
show the reaction diagram
-
38.0% conversion after 4 h at standard reaction conditions: 2 mM substrate, 0.013 U NTD, pH 7.5, 25C, 40 mM (NH4)2SO4, 0.5 mg/ml bovine serum albumin
-
-
r
hypoxanthine + deoxycytidine
deoxyinosine + cytosine
show the reaction diagram
-
51.9% conversion after 4 h at standard reaction conditions: 2 mM each substrate, 0.013 U NTD, pH 7.5, 25C, 40 mM (NH4)2SO4, 0.5 mg/ml bovine serum albumin
-
-
r
hypoxanthine + deoxyguanosine
deoxyinosine + guanine
show the reaction diagram
-
25.2% conversion after 4 h at standard reaction conditions: 2 mM substrate, 0.013 U NTD, pH 7.5, 25C, 40 mM (NH4)2SO4, 0.5 mg/ml bovine serum albumin
-
-
r
hypoxanthine + deoxyuridine
deoxyinosine + uracil
show the reaction diagram
-
65.6% conversion after 4 h at standard reaction conditions: 2 mM substrate, 0.013 U NTD, pH 7.5, 25C, 40 mM (NH4)2SO4, 0.5 mg/ml bovine serum albumin
-
-
r
hypoxanthine + thymidine
deoxyinosine + thymine
show the reaction diagram
-
76.8% conversion after 4 h at standard reaction conditions: 2 mM substrate, 0.013 U NTD, pH 7.5, 25C, 40 mM (NH4)2SO4, 0.5 mg/ml bovine serum albumin
-
-
r
purine base + 2-deoxy-D-ribosyl-base
purine deoxyribonucleoside + base
show the reaction diagram
-
-
preferred over pyrimidine deoxyribonucleoside synthesis
-
r
pyrimidine base + 2-deoxy-D-ribosyl-base
pyrimidine deoxyribonucleoside + base
show the reaction diagram
-
-
-
-
r
thymidine + 1-deaza-8-aza-adenine
thymine + 2'-deoxy-1-deaza-8-aza-adenosine
show the reaction diagram
-
21% of substrate converted into deoxynucleoside after 2 h
-
-
?
thymidine + 1-deaza-adenine
thymine + 2'-deoxy-1-deaza-adenosine
show the reaction diagram
-
6.2% of substrate converted into deoxynucleoside after 2 h
-
-
?
thymidine + 2,6-diaminopurine
thymine + 9-(2-deoxy-beta-D-ribofuranosyl)-2,6-diaminopurine
show the reaction diagram
-
53.4% of substrate converted into deoxynucleoside after 2 h
-
-
?
thymidine + 2,6-dichloropurine
thymine + 9-(2-deoxy-beta-D-ribofuranosyl)-2,6-dichloropurine
show the reaction diagram
-
-
-
-
r
thymidine + 2-amino-6-methyl-mercaptopurine
thymine + 9-(2-deoxy-beta-D-ribofuranosyl)-2-amino-6-methylmercaptopurine
show the reaction diagram
-
-
-
-
r
thymidine + 2-aminopurine
thymine + 9-(2-deoxy-beta-D-ribofuranosyl)-2-aminopurine
show the reaction diagram
-
-
-
-
r
thymidine + 2-pyrimidinol
thymine + ?
show the reaction diagram
-
-
-
-
?
thymidine + 2-thio-4-pyrimidinal
thymine + ?
show the reaction diagram
-
-
-
-
?
thymidine + 2-thio-5-methyl-4-pyrimidinol
thymine + ?
show the reaction diagram
-
-
-
-
?
thymidine + 3-deaza-8-aza-2-aminopurine
thymine + 9-(2-deoxy-beta-D-ribofuranosyl)-3-deaza-8-aza-aminopurine
show the reaction diagram
-
39.7% of substrate converted into deoxynucleoside after 2 h
-
-
?
thymidine + 3-deaza-8-aza-adenine
thymine + 2'-deoxy-3-deaza-8-aza-adenosine
show the reaction diagram
-
3.5% of substrate converted into deoxynucleoside after 2 h
-
-
?
thymidine + 4,6-pyrimidinediol
thymine + ?
show the reaction diagram
-
-
-
-
?
thymidine + 4-amino-2-pyrimidinethiol
thymine + ?
show the reaction diagram
-
-
-
-
?
thymidine + 4-amino-5-carboxamide-imidazole
thymine + ?
show the reaction diagram
-
12.3% of substrate converted into deoxynucleoside after 2 h
-
-
?
thymidine + 4-amino-6-pyrimidinol
thymine + ?
show the reaction diagram
-
-
-
-
?
thymidine + 4-amino-pyrazolopyrimidine
thymine + ?
show the reaction diagram
-
0.37% of substrate converted into deoxynucleoside after 2 h
-
-
?
thymidine + 4-methyl-2-pyrimidinol
thymine + ?
show the reaction diagram
-
-
-
-
?
thymidine + 4-pyrimidinol
thymine + ?
show the reaction diagram
-
-
-
-
?
thymidine + 5,6-dimethyl-benzimidazole
thymine + ?
show the reaction diagram
-
37.7% of substrate converted into deoxynucleoside after 2 h
-
-
?
thymidine + 5-bromouracil
thymine + 2'-deoxy-5-bromouridine
show the reaction diagram
-
-
-
-
?
thymidine + 5-fluorouracil
thymine + 2'-deoxy-5-fluorouridine
show the reaction diagram
-
-
-
-
?
thymidine + 5-hydroxyuracil
thymine + 2'-deoxy-5-hydroxyuridine
show the reaction diagram
-
-
-
-
?
thymidine + 5-methylcytosine
thymine + 2'-deoxy-5-methylcytosine
show the reaction diagram
-
-
-
-
?
thymidine + 5-nitrouracil
thymine + 2'-deoxy-5-nitrouridine
show the reaction diagram
-
-
-
-
?
thymidine + 5-thiouracil
thymine + 2'-deoxy-5-thiouridine
show the reaction diagram
-
-
-
-
?
thymidine + 6-benzylaminopurine
thymine + 9-(2-deoxy-beta-D-ribofuranosyl)-6-benzylaminopurine
show the reaction diagram
-
-
-
-
r
thymidine + 6-chloropurine
thymine + 9-(2-deoxy-beta-D-ribofuranosyl)-6-chloropurine
show the reaction diagram
-
-
-
-
r
thymidine + 6-dimethylaminopurine
thymine + 9-(2-deoxy-beta-D-ribofuranosyl)-6-dimethylaminopurine
show the reaction diagram
-
-
-
-
r
thymidine + 6-iodopurine
thymine + 9-(2-deoxy-beta-D-ribofuranosyl)-6-iodopurine
show the reaction diagram
-
65.6% of substrate converted into deoxynucleoside after 2 h
-
-
?
thymidine + 6-mercaptoguanine
thymine + 6-mercaptoguanosine
show the reaction diagram
-
52.1% of substrate converted into deoxynucleoside after 2 h
-
-
?
thymidine + 6-mercatopurine
thymine + 9-(2-deoxy-beta-D-ribofuranosyl)-6-mercaptopurine
show the reaction diagram
-
56.5% of substrate converted into deoxynucleoside after 2 h
-
-
?
thymidine + 6-methoxypurine
thymine + 9-(2-deoxy-beta-D-ribofuranosyl)-6-methoxypurine
show the reaction diagram
-
-
-
-
r
thymidine + 6-methyl-cis-triazine-3,5-diol
thymine + ?
show the reaction diagram
-
-
-
-
?
thymidine + 6-methylaminopurine
thymine + 9-(2-deoxy-beta-D-ribofuranosyl)-6-methylaminopurine
show the reaction diagram
-
-
-
-
r
thymidine + 6-methylpurine
thymine + 9-(2-deoxy-beta-D-ribofuranosyl)-6-methylpurine
show the reaction diagram
-
-
-
-
r
thymidine + 6-methylthio-8-trifluoromethylpurine
thymine + 9-(2-deoxy-beta-D-ribofuranosyl)-6-methylthio-8-trifluoromethylpurine
show the reaction diagram
-
-
-
-
?
thymidine + 6-n-hexylaminopurine
thymine + 9-(2-deoxy-beta-D-ribofuranosyl)-6-n-hexylaminopurine
show the reaction diagram
-
-
-
-
r
thymidine + 6-n-hexylmercaptopurine
thymine + 9-(2-deoxy-beta-D-ribofuranosyl)-6-n-hexylmercaptopurine
show the reaction diagram
-
-
-
-
r
thymidine + 6-phenylaminopurine
thymine + 9-(2-deoxy-beta-D-ribofuranosyl)-6-phenylaminopurine
show the reaction diagram
-
-
-
-
r
thymidine + 7-hydroxy-1,2,5-selenadiazolopyridine
thymine + ?
show the reaction diagram
-
29.2% of substrate converted into deoxynucleoside after 2 h
-
-
?
thymidine + 8-aza-2,6-diaminopurine
thymine + 9-(2-deoxy-beta-D-ribofuranosyl)-8-aza-2,6-diaminopurine
show the reaction diagram
-
59.6% of substrate converted into deoxynucleoside after 2 h
-
-
?
thymidine + 8-azaguanine
thymine + 2'-deoxy-8-azaguanosine
show the reaction diagram
-
16.0% of substrate converted into deoxynucleoside after 2 h
-
-
?
thymidine + 8-azaxanthine
thymine + 2'-deoxy-8-azaxanthosine
show the reaction diagram
-
-
-
-
?
thymidine + 8-bromoadenine
thymine + 2'-deoxy-8-bromoadenosine
show the reaction diagram
-
-
-
?
thymidine + 8-chloroadenine
thymine + 2'-deoxy-8-chloroadenosine
show the reaction diagram
-
-
3-deoxyribonucleoside + 9-deoxyribonucleoside
?
thymidine + 8-chlorotheophylline
thymine + 9-(2-deoxy-beta-D-ribofuranosyl)-8-chlorotheophylline
show the reaction diagram
-
-
-
-
?
thymidine + 8-methyladenine
thymine + 2'-deoxy-8-methyladenosine
show the reaction diagram
-
-
-
-
?
thymidine + 8-trifluoromethyladenine
thymine + 2'-deoxy-8-trifluoromethyladenosine
show the reaction diagram
-
-
-
-
?
thymidine + adenine
2'-deoxy-D-ribosyl-adenine + thymine
show the reaction diagram
thymidine + adenine
2'-deoxyadenosine + thymine
show the reaction diagram
thymidine + adenine
thymine + 2'-deoxyadenosine
show the reaction diagram
-
-
-
-
r
thymidine + adenine
thymine + adenosine
show the reaction diagram
thymidine + benzimidazole
thymine + ?
show the reaction diagram
-
33.1% of substrate converted into deoxynucleoside after 2 h
-
-
?
thymidine + cis-triazine-3,5-diol
thymine + ?
show the reaction diagram
-
-
-
-
?
thymidine + cytosine
thymine + 2'-deoxycytidine
show the reaction diagram
thymidine + guanine
deoxyguanine + thymine
show the reaction diagram
-
-
-
-
?
thymidine + guanine
thymine + 2'-deoxyguanosine
show the reaction diagram
thymidine + hypoxanthine
deoxyinosine + thymine
show the reaction diagram
-
-
-
-
?
thymidine + hypoxanthine
thymine + 2'-deoxyinosine
show the reaction diagram
-
-
-
-
r
thymidine + purine
thymine + 9-(2-deoxy-beta-D-ribofuranosyl)-purine
show the reaction diagram
-
-
-
-
r
thymidine + theophylline
thymine + 2'-deoxytheophylline riboside
show the reaction diagram
-
-
-
-
?
thymidine + uracil
deoxyuridine + thymine
show the reaction diagram
-
-
-
-
?
thymidine + uracil
thymine + 2'-deoxyuridine
show the reaction diagram
-
-
-
-
?
thymidine + xanthine
9-deoxyribosylxanthine + 7-deoxyribosylxanthine
show the reaction diagram
-
-
20% 9-deoxyribosylxanthine + 80% 7-deoxyribosylxanthine
?
thymidine + xanthine
thymine + xanthosine
show the reaction diagram
-
-
-
-
r
thymine + deoxyadenosine
thymidine + adenine
show the reaction diagram
-
22.7% conversion after 4 h at standard reaction conditions: 2 mM each substrate, 0.013 U NTD, pH 7.5, 25C, 40 mM (NH4)2SO4, 0.5 mg/ml bovine serum albumin
-
-
r
thymine + deoxycytidine
thymidine + cytosine
show the reaction diagram
-
38.3% conversion after 4 h at standard reaction conditions: 2 mM each substrate, 0.013 U NTD, pH 7.5, 25C, 40 mM (NH4)2SO4, 0.5 mg/ml bovine serum albumin
-
-
r
thymine + deoxyguanosine
thymidine + guanine
show the reaction diagram
-
17.7% conversion after 4 h at standard reaction conditions: 2 mM each substrate, 0.013 U NTD, pH 7.5, 25C, 40 mM (NH4)2SO4, 0.5 mg/ml bovine serum albumin
-
-
r
thymine + deoxyinosine
thymidine + hypoxanthine
show the reaction diagram
-
19.5% conversion after 4 h at standard reaction conditions: 2 mM each substrate, 0.013 U NTD, pH 7.5, 25C, 40 mM (NH4)2SO4, 0.5 mg/ml bovine serum albumin, equilibrium reached after 2 h
-
-
r
thymine + deoxyuridine
thymidine + uracil
show the reaction diagram
-
53.7% conversion after 4 h at standard reaction conditions: 2 mM each substrate, 0.013 U NTD, pH 7.5, 25C, 40 mM (NH4)2SO4, 0.5 mg/ml bovine serum albumin
-
-
r
uracil + deoxyadenosine
deoxyuridine + adenine
show the reaction diagram
-
17.1% conversion after 4 h at standard reaction conditions: 2 mM each substrate, 0.013 U NTD, pH 7.5, 25C, 40 mM (NH4)2SO4, 0.5 mg/ml bovine serum albumin
-
-
r
uracil + deoxycytidine
deoxyuridine + cytosine
show the reaction diagram
-
37.7% conversion after 4 h at standard reaction conditions: 2 mM each substrate, 0.013 U NTD, pH 7.5, 25C, 40 mM (NH4)2SO4, 0.5 mg/ml bovine serum albumin
-
-
r
uracil + deoxyguanosine
deoxyuridine + guanine
show the reaction diagram
-
16.1% conversion after 4 h at standard reaction conditions: 2 mM each substrate, 0.013 U NTD, pH 7.5, 25C, 40 mM (NH4)2SO4, 0.5 mg/ml bovine serum albumin
-
-
r
uracil + deoxyinosine
deoxyuridine + hypoxanthine
show the reaction diagram
-
23.8% conversion after 4 h at standard reaction conditions: 2 mM each substrate, 0.013 U NTD, pH 7.5, 25C, 40 mM (NH4)2SO4, 0.5 mg/ml bovine serum albumin
-
-
r
uracil + thymidine
deoxyuridine + thymine
show the reaction diagram
-
43.0% conversion after 4 h at standard reaction conditions: 2 mM each substrate, 0.013 U NTD, pH 7.5, 25C, 40 mM (NH4)2SO4, 0.5 mg/ml bovine serum albumin
-
-
r
additional information
?
-
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
2'-deoxy-D-ribosyl-uracil + adenine
2'-deoxy-D-ribosyl-adenine + uracil
show the reaction diagram
purine base + 2-deoxy-D-ribosyl-base
purine deoxyribonucleoside + base
show the reaction diagram
-
-
preferred over pyrimidine deoxyribonucleoside synthesis
-
r
pyrimidine base + 2-deoxy-D-ribosyl-base
pyrimidine deoxyribonucleoside + base
show the reaction diagram
-
-
-
-
r
additional information
?
-
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
K+
-
enhances base exchange activity and thermostability, 1.66-fold activation by 1 mM KCl (reaches plateau at 20 mM)
NH4+
-
enhances base exchange activity and thermostability, 1.43-fold activation by 1 mM (NH4)2SO4 (reaches plateau at 20 mM)
Rb+
-
enhances base exchange activity, 1.51-fold activation by 1 mM RbCl
additional information
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
6-methylthio-GMP
-
competitive versus dGMP
amino-pyrrolo-pyrimidine
-
12.1 mM, complete inhibition of the reaction with adenine and deoxycytidine
Ca2+
-
inhibits base exchange activity at 1 mM
CO2
-
reduces base exchange activity by 87-99% at 1 mM
Cu2+
-
reduces base exchange activity by 87-99% at 1 mM
deoxyadenosine
deoxyinosine
-
-
GMP
-
competitive versus dGMP
Hydroxypyrrolopyrimidine
-
0.33 mM, 66% inhibition of the reaction with adenine and deoxycytidine
-
hypoxanthine
-
-
imidazole
-
12.1 mM, 76% inhibition of the reaction with adenine and deoxycytidine
Mg2+
-
inhibits base exchange activity at 1mM
Mn2+
-
reduces base exchange activity by 87-99% at 1 mM
NaCl
-
70% activity remains at 1 M NaCl
ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
K+
-
K+-activated type II enzyme: binding of K+ is required to enhance activity through induced conformational change
additional information
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.38 - 0.92
2',3'-dideoxycytidine
0.25 - 22
2'-deoxycytidine
1.14
2'-deoxyuridine
-
pH 6.5, 40C
0.0073 - 0.48
adenine
0.04
adenosine
-
reaction with deoxyinosine
0.05 - 10.79
cytosine
0.25
dAMP
-
pH 7.5, 37C, recombinant wild-type enzyme
4
dCMP
-
pH 7.5, 37C, recombinant wild-type enzyme
0.092 - 0.45
deoxyadenosine
0.09 - 0.55
deoxycytidine
0.16 - 0.37
deoxyguanosine
0.041 - 3.5
deoxyinosine
0.048 - 5
dGMP
0.45
dIMP
-
pH 7.5, 37C, recombinant wild-type enzyme
50
dTMP
-
above, pH 7.5, 37C, recombinant wild-type enzyme
15
dUMP
-
pH 7.5, 37C, recombinant wild-type enzyme
0.03 - 0.52
Guanine
0.086
hypoxanthine
-
-
0.05 - 0.63
thymidine
0.13
thymine
-
reaction with deoxyinosine
additional information
additional information
-
kinetics and substrate specifiicty
-
TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.047 - 10.4
2',3'-dideoxycytidine
0.3 - 62.2
2'-deoxycytidine
483
2'-deoxyuridine
Lactobacillus reuteri
-
pH 6.5, 40C
2083
cytosine
Lactobacillus reuteri
-
pH 6.5, 40C
0.0066
dAMP
Rattus norvegicus
-
pH 7.5, 37C, recombinant wild-type enzyme
0.0231
dCMP
Rattus norvegicus
-
pH 7.5, 37C, recombinant wild-type enzyme
0.0178 - 0.0297
dGMP
0.0066
dIMP
Rattus norvegicus
-
pH 7.5, 37C, recombinant wild-type enzyme
0.158
dUMP
Rattus norvegicus
-
pH 7.5, 37C, recombinant wild-type enzyme
additional information
additional information
Lactobacillus leichmannii
-
-
-
kcat/KM VALUE [1/mMs-1]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.12 - 13
2',3'-dideoxycytidine
0.0136 - 250
2'-deoxycytidine
424
2'-deoxyuridine
Lactobacillus reuteri
-
pH 6.5, 40C
1705
193
cytosine
Lactobacillus reuteri
-
pH 6.5, 40C
489
Ki VALUE [mM]
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.01
6-methylthio-GMP
-
pH 7.5, 37C, recombinant wild-type enzyme, versus dGMP
0.021 - 0.039
adenine
0.092 - 0.43
deoxyadenosine
0.095
deoxycytidine
-
-
0.34
deoxyinosine
-
-
0.02
GMP
-
pH 7.5, 37C, recombinant wild-type enzyme, versus dGMP
0.079
hypoxanthine
-
-
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
0.017
wild-type, with 2',3'-dideoxythymidine and adenine as substrates
0.02
mutant G9S, with 2'-deoxyinosine and adenine as substrates
0.04
mutant G9S, with 2',3'-dideoxyinosine and adenine as substrates; wild-type, with 2',3'-dideoxycytidine and hypoxanthine as substrates
0.056
wild-type, with 2',3'-dideoxyuridine and cytosine as substrates
0.19
wild-type, with 2',3'-dideoxyuridine and cytosine as substrates
0.2
mutant G9S, with 2',3'didehydro-2',3'-dideoxythymidine and adenine as substrates
0.25
mutant G9S, with 3'-dideoxythymidine and adenine as substrates
0.4
mutant G9S, with 2',3'-dideoxyguanosine and thymine as substrates
0.42
mutant A15T, 2',3'-dideoxyadenosine and thymine as substrates
0.46
mutant A15T, 2'-deoxyadenosine and thymine as substrates
0.47
mutant G9S, with 2'-deoxyinosine and adenine as substrates
0.57
mutant G9S, with 2'-deoxyguanosine and thymine
0.6
mutant G9S, with 2',3'-dideoxyguanosine and thymine as substrates
1.04
mutant G9S, with 2'-deoxythymidine and adenine as substrates
1.08
wild-type, with 2'-deoxyinosine and adenine as substrates
1.25
mutant G9S, with 2'-deoxycytidine and hypoxanthine as substrates
1.3
mutant G9S, with 2',3'-dideoxyuridine and cytosine as substrates
1.35
mutant A15T, 2',3'-dideoxyadenosine and cytosine as substrates
1.47
mutant A15T, 2',3'-dideoxycytidine and thymine as substrates
1.6
mutant G9S, with 2',3'-dideoxyuridine and cytosine as substrates
3.1
mutant G9S, with 2'-deoxyuridine and cytosine as substrates
3.6
wild-type, with 2'-deoxyinosine and adenine as substrates
4.95
wild-type, with 2'-deoxyguanosine and thymine
5.2
wild-type, with 2'-deoxyguanosine and thymine
6
mutant G9S, with 2',3'-dideoxycytidine and adenine as substrates
6.67
-
equals 100%, purified protein, in presence of 0.5 mg/ml bovine serum albumin, pH 7.5, 10 min, 25C
7.11
mutant A15T, 2'-deoxycytidine and thymine as substrates
7.75
-
reaction with thymidine and cytosine
8.4
wild-type, 2'-deoxyadenosine and thymine as substrates
11.9
wild-type, with 2'-deoxythymidine and adenine as substrates
12.7
mutant G9S, with 2'-deoxyuridine and cytosine as substrates
27.1
mutant G9S, with 2'-deoxycytidine and adenine as substrates
29
wild-type, with 2',3'-dideoxycytidine and adenine as substrates
32
wild-type, 2'-deoxycytidine and thymine as substrates
33.2
wild-type, 2'-deoxyadenosine and cytosine as substrates
36.8
-
DRTase II, reaction with thymidine + adenine
40.2
wild-type, with 2'-deoxyuridine and cytosine as substrates
42.5
wild-type, with 2'-deoxyuridine and cytosine as substrates
42.9
-
DRTase I, reaction with deoxyinosine and adenine
43
-
reaction with deoxycytidine and adenine
173
wild-type, with 2'-deoxycytidine and adenine as substrates
additional information
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
7.5
-
assay at
7.7
-
reaction with deoxyinosine and guanine
pH RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
5 - 8
-
50% of maximal activity at pH 7.0 with substrate dCMP, 20% with dGMP, 10% of maximal activity at pH 8.0 with both substrates
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
30
-
assay at, pH 7.5
37
-
assay at
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
18080
native enzyme, mass spectrometry
18110
mutant, mass spectrometry
18290
-
theoretical molecular weight
18900
native enzyme, mass spectrometry
18930
mutant, mass spectrometry
20440
-
monomeric enzyme, equilibrium sedimentation ultracentrifugation
25500
-
according to migration in SDS-12.5% polyacrylamide gel electrophoresis
40520
-
monomeric enzyme, equilibrium sedimentation ultracentrifugation
69000
-
gel filtration chromatography
82000
-
gel filtration
110000
112700
-
sedimentation equilibrium experiments
120000
-
DRTase I, nondenaturing PAGE
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
dimer
-
2 * 17649, unmodified recombinant enzyme, mass spectrometry, 2 * 17649, unmodified recombinant enzyme, mass spectrometry, 2 * 20440, His-tagged recombinant enzyme, SDS-PAGE
hexamer
homotetramer
-
4 * 17250, elution as single peak from the preparative gel filtration column
monomer
-
1 * 17649, unmodified recombinant enzyme, mass spectrometry, 1 * 19812, His-tagged recombinant enzyme, mass spectrometry, 1 * 20440, His-tagged recombinant enzyme, SDS-PAGE
additional information
Crystallization/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
microdiffusion by means of equilibrium dialysis is used to crystallize the enzyme
-
purified recombinant selenomethionine-labeled enzyme free or in complex with benzo[cd]indol-2(1H)-one, 5-aminoisoquinoline, 2-ethylbenzyl alcohol, or 1-methylquinoline-2(1H)-one, fragment cocktail crystallography, X-ray diffraction structure determination and analysis at 1.5-1.8 A resolution, SAD X-ray techniques
-
pH STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
4
-
61C, 30 min, about 30% loss of activity
638040
4 - 8
-
enzyme is completely stable in this pH interval
721340
8
-
61C, 30 min, about 15% loss of activity
638040
8.5
-
61C, 30 min, about 70% of maximal activity
638040
TEMPERATURE STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
25
-
pH 7.5, 20 min, 60% activity lost, in presence of 10 mM (NH4)2SO4 only 20% activity lost, in presence of 500 mM KCL only 6% activity lost
30
-
pH 7.5, 20 min, in presence of 500 mM (NH4)2SO4 fully active
35
-
and more, pH 7.5, 20 min, 75% activity lost
50
-
stable up to
55
-
pH 6.3, 10 min, stable below
64
-
melting temperature
90
-
pH 6.3, 10 min, 75% loss of activity
additional information
-
(NH4)2SO4 is more thermostabilizing than KCl
GENERAL STABILITY
ORGANISM
UNIPROT
LITERATURE
crystals are stable to X-rays for at least 5 days
-
ORGANIC SOLVENT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
2-propanol
-
enzyme immobilized on Sepabeads shows 1.2-1.4fold enhanced activity at 20% of methanol, ethanol, 2-propanol, ethylene glycol, and acetone, and at 10% and 30% acetonitrile. Highest increase in activity is obtained in presence of 20% acetonitrile
Acetone
-
enzyme immobilized on Sepabeads shows 1.2-1.4fold enhanced activity at 20% of methanol, ethanol, 2-propanol, ethylene glycol, and acetone, and at 10% and 30% acetonitrile. Highest increase in activity is obtained in presence of 20% acetonitrile
acetonitrile
-
enzyme immobilized on Sepabeads shows 1.2-1.4fold enhanced activity at 20% of methanol, ethanol, 2-propanol, ethylene glycol, and acetone, and at 10% and 30% acetonitrile. Highest increase in activity is obtained in presence of 20% acetonitrile
Ethanol
-
enzyme immobilized on Sepabeads shows 1.2-1.4fold enhanced activity at 20% of methanol, ethanol, 2-propanol, ethylene glycol, and acetone, and at 10% and 30% acetonitrile. Highest increase in activity is obtained in presence of 20% acetonitrile
Ethylene glycol
-
enzyme immobilized on Sepabeads shows 1.2-1.4fold enhanced activity at 20% of methanol, ethanol, 2-propanol, ethylene glycol, and acetone, and at 10% and 30% acetonitrile. Highest increase in activity is obtained in presence of 20% acetonitrile
Methanol
-
enzyme immobilized on Sepabeads shows 1.2-1.4fold enhanced activity at 20% of methanol, ethanol, 2-propanol, ethylene glycol, and acetone, and at 10% and 30% acetonitrile. Highest increase in activity is obtained in presence of 20% acetonitrile
STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
-20C, purified recombinant enzyme, 1 mg/ml, at least 1 year without loss of activity
-
-50C, stable for several months either in the lyophilized form or disolved in freshly distilled water
-
Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
DRTase I and DRTAse II
-
preclearing of bacterial lysate with 40% ammonium sulfate and subsequent precipitation of proteins with 90% ammonium sulfate, gel filtration of dissolved protein on Sephacryl S-200 column, combination of fractions with N-deoxyribosyltransferase activity, precipitation of proteins with 90% ammonium sulfate, dialysis of dissolved proteins and concentration by ultrafiltration, second gel filtration on Sephacryl S-200 column, combination of active fractions and concentration by ultrafiltration, recovery of 70.4% of activity, storage at -20C in 50% glycerol
-
recombinant His-tagged wild-type and selenomethionine-labeled enzymes from Escherichia coli to homogeneity by nickel affinity chromatography and gel filtration
-
recombinant unmodified enzyme from Escherichia coli strain Bli5 (BL21 (DE3) pDIA17) by anion exchange chromatography and gel filtration, recombinant His-tagged enzyme by nickel affinity chromatography, over 95% purity
-
Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
expression in Escherichia coli
expression of His-tagged wild-type and selenomethionine-labeled enzymes in Escherichia coli
-
expression of the catalytically inactive mutant E98A in Escherichia coli
-
gene Rc1, expression of soluble unmodified enzyme and His-tagged enzymes in Escherichia coli strain Bli5 (BL21 (DE3) pDIA17)
-
in pET-22b(+) for expression in Escherichia coli BL21(DE3); in pGEM-T for cloning
-
ENGINEERING
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
A15V
can not improve 2',3'-dideoxyribose transfer reaction, kcat is diminished as compared to wild-type
A15C
-
kcat is diminished as compared to wild-type
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A15S
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activity with 2'-deoxycytidine and adenine as substrates is closer to that of the wild-type enzyme than that of the A15T mutant, kcat is diminished as compared to wild-type
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A15T
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mutation allows conversion of the nucleoside 2'deoxyribosyltransferase activity to a nucleoside 2',3'-dideoxy- or 2',3'-didehydro,2',3'-dideoxyribosyltransferase activity, kcat is diminished as compared to wild-type
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A15V
-
can not improve 2',3'-dideoxyribose transfer reaction, kcat is diminished as compared to wild-type
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E98A
-
catalytically inactive mutant
G9C
can not improve 2',3'-dideoxyribose transfer reaction, kcat is diminished as compared to wild-type
G9T
Ser remains a better substituent for Gly-9 than Thr, kcat is diminished as compared to wild-type
G9W
can not improve 2',3'-dideoxyribose transfer reaction, kcat is diminished as compared to wild-type
E105A
-
site-directed mutagenesis, the mutant shows about 200fold reduced activity and a 170fold increased Km compared to the wild-type enzyme
additional information
APPLICATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
medicine
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the low substrate specificity of the enzyme is used advantageously for synthesis of nucleoside analogs, some of them of medical interest
synthesis
Show AA Sequence (172 entries)
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