Information on EC 2.4.2.47 - arabinofuranan 3-O-arabinosyltransferase

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The expected taxonomic range for this enzyme is: Mycobacterium tuberculosis

EC NUMBER
COMMENTARY
2.4.2.47
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RECOMMENDED NAME
GeneOntology No.
arabinofuranan 3-O-arabinosyltransferase
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REACTION
REACTION DIAGRAM
COMMENTARY
ORGANISM
UNIPROT ACCESSION NO.
LITERATURE
Adds an alpha-D-arabinofuranosyl group from trans,octacis-decaprenylphospho-beta-D-arabinofuranose at the 3-O-position of an alpha-(1->5)-arabinofuranan chain attached to a beta-(1->5)-galactofuranan chain
show the reaction diagram
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PATHWAY
KEGG Link
MetaCyc Link
mycolyl-arabinogalactan-peptidoglycan complex biosynthesis
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SYSTEMATIC NAME
IUBMB Comments
alpha-(1->5)-arabinofuranan:trans,octacis-decaprenylphospho-beta-D-arabinofuranose 3-O-alpha-D-arabinofuranosyltransferase
Isolated from Mycobacterium smegmatis. Involved in the formation of the cell wall in mycobacteria.
SYNONYMS
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
1,3-alpha-arabinosyltransferase
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AftC
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arabinofuranosyltransferase C
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branching alpha(1->3) arabinofuranosyltransferase
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Rv2673
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gene name
ORGANISM
COMMENTARY
LITERATURE
SEQUENCE CODE
SEQUENCE DB
SOURCE
GENERAL INFORMATION
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
metabolism
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the enzyme is involved in the synthesis of the internal alpha-(1->3)-branching of the arabinan domain of arabinogalactan
SUBSTRATE
PRODUCT                      
REACTION DIAGRAM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
(Substrate)
LITERATURE
(Substrate)
COMMENTARY
(Product)
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
(2Z,6Z)-farnesylphospho-alpha-D-arabinofuranoside + octyl (alpha-D-Araf)2-(1->3,5)-alpha-D-Araf-(1->5)-alpha-D-Araf-(1->5)-alpha-D-Araf
?
show the reaction diagram
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?
(2Z,6Z)-farnesylphospho-alpha-D-arabinofuranoside + octyl alpha-D-Araf-(1->5)-alpha-D-Araf-(1->5)-alpha-D-Araf-(1->5)-alpha-D-Araf-(1->5)-alpha-D-Araf
?
show the reaction diagram
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?
beta-D-arabinofuranosyl-1-monophosphoryl-decaprenol + alpha-D-arabinofuranosyl-(1->5)-alpha-D-arabinofuranosyl-(1->5)-alpha-D-arabinofuranosyl-(1->5)-alpha-arabinofuranosyl-(1->5)-alpha-D-arabinofuranosyl-O-(CH2)8NH2
?
show the reaction diagram
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?
decaprenylphospho-alpha-D-arabinofuranoside + octyl alpha-D-Araf-(1->5)-alpha-D-Araf-(1->5)-alpha-D-Araf-(1->5)-alpha-D-Araf-(1->5)-alpha-D-Araf
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show the reaction diagram
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?
ditrans,tetracis-heptaprenylphospho-alpha-D-arabinofuranoside + octyl alpha-D-Araf-(1->5)-alpha-D-Araf-(1->5)-alpha-D-Araf-(1->5)-alpha-D-Araf-(1->5)-alpha-D-Araf
?
show the reaction diagram
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?
additional information
?
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no activity with octyl alpha-D-Araf-(1->5)-alpha-D-Araf and dipalmitoyl phosphatidylcholine
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additional information
?
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the enzyme catalyses the addition of an alpha(1->3)-arabinofuranosyl unit via an alpha(1->3) arabinofuranosyltransferase
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additional information
?
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the enzyme introduces alpha-(1->3) branch points in the internal arabinan domain in both arabinogalactan and lipoarabinomannan synthesis. The enzyme can only transfer D-arabinofuranose when a minimum of three arabinofuranosyl residues are present in a linear structure in order to introduce an alpha-D-Araf-(1->3) branch point
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ACTIVATING COMPOUND
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
IMAGE
additional information
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AftC is active only when reconstituted in a proteoliposome
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LOCALIZATION
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
GeneOntology No.
LITERATURE
SOURCE
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trans-membrane protein
Manually annotated by BRENDA team
SUBUNITS
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
?
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x * 38000, SDS-PAGE
Purification/COMMENTARY
ORGANISM
UNIPROT ACCESSION NO.
LITERATURE
metal affinity column column chromatography
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Cloned/COMMENTARY
ORGANISM
UNIPROT ACCESSION NO.
LITERATURE
expressed in Mycobacterium smegmatis mc2155/pJAM/Rv2673 cells
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expressed in Mycobacterium smegmatis strain mc2155 MSMEG2785
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