Information on EC 2.4.2.42 - UDP-D-xylose:beta-D-glucoside alpha-1,3-D-xylosyltransferase

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The expected taxonomic range for this enzyme is: Eutheria

EC NUMBER
COMMENTARY
2.4.2.42
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RECOMMENDED NAME
GeneOntology No.
UDP-D-xylose:beta-D-glucoside alpha-1,3-D-xylosyltransferase
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REACTION
REACTION DIAGRAM
COMMENTARY
ORGANISM
UNIPROT ACCESSION NO.
LITERATURE
UDP-alpha-D-xylose + Glcbeta-Ser53-EGF-like domain of bovine factor IX(45-87) = UDP + Xylalpha(1-3)Glcbeta-Ser53-EGF-like domain of bovine factor IX(45-87)
show the reaction diagram
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SYSTEMATIC NAME
IUBMB Comments
UDP-D-xylose:beta-D-glucoside alpha-1,3-D-xylosyltransferase
The enzyme is involved in the biosynthesis of the Xylalpha(1-3)Xylalpha(1-3)Glcbeta-1-O-Ser on epidermal growth factor-like domains [1].
SYNONYMS
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
beta-glucoside alpha-1,3-xylosyltransferase
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UDP-D-xylose:beta-D-glucoside alpha-1,3-D-xylosyltransferase
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UDP-D-xylose:beta-D-glucoside alpha-1,3-D-xylosytransferase
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ORGANISM
COMMENTARY
LITERATURE
SEQUENCE CODE
SEQUENCE DB
SOURCE
SUBSTRATE
PRODUCT                      
REACTION DIAGRAM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
(Substrate)
LITERATURE
(Substrate)
COMMENTARY
(Product)
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
UDP-D-xylose + 2-(pyridin-2-ylamino)ethyl beta-D-glucopyranoside
UDP + 2-(pyridin-2-ylamino)ethyl 3-O-alpha-D-xylopyranosyl-beta-D-glucopyranoside
show the reaction diagram
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-
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-
?
UDP-D-xylose + 2-(pyridin-2-ylamino)ethyl beta-D-glucopyranoside
UDP + 2-(pyridin-2-ylamino)ethyl 3-O-alpha-D-xylopyranosyl-beta-D-glucopyranoside
show the reaction diagram
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-
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-
?
UDP-D-xylose + D-glucose
UDP + Xylalpha(1-3)Glc
show the reaction diagram
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-
?
UDP-D-xylose + Glcbeta-Ser53-EGF-like domain of bovine factor IX(45-87)
UDP + Xylalpha(1-3)Glcbeta-Ser53-EGF-like domain of bovine factor IX(45-87)
show the reaction diagram
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the enzyme is involved in the biosynthesis of the Xylalpha(1-3)Xylalpha(1-3)Glcbeta-1-O-Ser on epidermal growth factor-like domains, activity is 12fold lower with reduced and alkylated Glc-EGF. This enzyme recognizes not only the glucose portion but also the tertiary structure of the EGF-like domain even though it transfers xylose to a glucose residue and not to peptide
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?
additional information
?
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the enzyme is appears to be involved in the biosynthesis of the Xylalpha(1-3)Glcbeta-Ser structure of glycoproteins such as human blood coagulation factors VII and IX
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additional information
?
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no activity with UDP-D-glucose
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NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
(Substrate)
LITERATURE
(Substrate)
COMMENTARY
(Product)
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
UDP-D-xylose + Glcbeta-Ser53-EGF-like domain of bovine factor IX(45-87)
UDP + Xylalpha(1-3)Glcbeta-Ser53-EGF-like domain of bovine factor IX(45-87)
show the reaction diagram
-
the enzyme is involved in the biosynthesis of the Xylalpha(1-3)Xylalpha(1-3)Glcbeta-1-O-Ser on epidermal growth factor-like domains
-
-
?
additional information
?
-
-
the enzyme is appears to be involved in the biosynthesis of the Xylalpha(1-3)Glcbeta-Ser structure of glycoproteins such as human blood coagulation factors VII and IX
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-
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METALS and IONS
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
Mn2+
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20 mM, 2fold increase in activity
additional information
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20 mM Ca2+, Mg2+ or Sr2+ has no effect on activity
INHIBITORS
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
IMAGE
Co2+
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20 mM, 85% loss of activity
Cu2+
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20 mM, complete loss of activity
Fe2+
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20 mM, complete loss of activity
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Ni2+
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20 mM, 90% loss of activity
KM VALUE [mM]
KM VALUE [mM] Maximum
SUBSTRATE
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
IMAGE
4.2
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2-(pyridin-2-ylamino)ethyl beta-D-glucopyranoside
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pH 7.2, 37°C
0.034
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Glcbeta-Ser53-EGF-like domain of bovine factor IX(45-87)
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pH 7.2, 37°C
SPECIFIC ACTIVITY [µmol/min/mg]
SPECIFIC ACTIVITY MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
0.075
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pH OPTIMUM
pH MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
7
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around
7.2
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pH RANGE
pH RANGE MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
6
8
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pH 6.0: about 30% of maximal activity, pH 8.0: about 70% of maximal activity
6.6
7.6
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pH 6.6: about 80% of maximal activity, pH 7.6: about 90% of maximal activity
TEMPERATURE OPTIMUM
TEMPERATURE OPTIMUM MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
37
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assay at
SOURCE TISSUE
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
SOURCE
LOCALIZATION
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
GeneOntology No.
LITERATURE
SOURCE
Purification/COMMENTARY
ORGANISM
UNIPROT ACCESSION NO.
LITERATURE