Information on EC 2.4.2.40 - zeatin O-beta-D-xylosyltransferase

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The expected taxonomic range for this enzyme is: Phaseolus vulgaris

EC NUMBER
COMMENTARY
2.4.2.40
-
RECOMMENDED NAME
GeneOntology No.
zeatin O-beta-D-xylosyltransferase
REACTION
REACTION DIAGRAM
COMMENTARY
ORGANISM
UNIPROT ACCESSION NO.
LITERATURE
UDP-D-xylose + zeatin = UDP + O-beta-D-xylosylzeatin
show the reaction diagram
does not act on UDP-glucose
-
-
-
UDP-D-xylose + zeatin = UDP + O-beta-D-xylosylzeatin
show the reaction diagram
localisation of site determining the substrate specificity for the UDP-sugar donors
-
REACTION TYPE
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
pentosyl group transfer
-
-
-
-
PATHWAY
KEGG Link
MetaCyc Link
Zeatin biosynthesis
-
SYSTEMATIC NAME
IUBMB Comments
UDP-D-xylose:zeatin O-beta-D-xylosyltransferase
Does not act on UDP-glucose (cf. EC 2.4.1.103 alizarin 2-beta-glucosyltransferase).
SYNONYMS
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
uridine diphosphoxylose-zeatin xylosyltransferase
-
-
-
-
xylosyltransferase, uridine diphosphoxylose-zeatin
-
-
-
-
zeatin O-xylosyltransferase
-
-
-
-
CAS REGISTRY NUMBER
COMMENTARY
110541-22-5
-
ORGANISM
COMMENTARY
LITERATURE
SEQUENCE CODE
SEQUENCE DB
SOURCE
no activity in Phaseolus lunatus
-
-
-
Manually annotated by BRENDA team
cv. Great Northern
-
-
Manually annotated by BRENDA team
cv. Great Northern; gene ZOX1
Uniprot
Manually annotated by BRENDA team
Phaseolus vulgaris x Phaseolus coccineus
-
-
-
Manually annotated by BRENDA team
SUBSTRATE
PRODUCT                      
REACTION DIAGRAM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
(Substrate)
LITERATURE
(Substrate)
COMMENTARY
(Product)
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
UDP-D-xylose + dihydrozeatin
UDP + O-beta-D-xylosyldihydrozeatin
show the reaction diagram
-
-
-
?
UDP-D-xylose + dihydrozeatin
UDP + O-beta-D-xylosyldihydrozeatin
show the reaction diagram
-
-
-
-
?
UDP-D-xylose + trans-zeatin
?
show the reaction diagram
Phaseolus vulgaris, Phaseolus vulgaris x Phaseolus coccineus
-
may be involved in the nuclear-cytoplasmic transport of cytokinins and related molecules or possibly, with chromatin of rapidly dividing cells
-
-
-
UDP-D-xylose + zeatin
UDP + O-beta-D-xylosylzeatin
show the reaction diagram
-
-
-
?
UDP-D-xylose + zeatin
UDP + O-beta-D-xylosylzeatin
show the reaction diagram
-
substrate specificity, absolutely specific for UDP-D-xylose
-
?
UDP-D-xylose + zeatin
UDP + O-beta-D-xylosylzeatin
show the reaction diagram
-
absolutely specific for UDP-D-xylose
-
?
UDP-D-xylose + zeatin
UDP + O-beta-D-xylosylzeatin
show the reaction diagram
P56725
absolutely specific for UDP-D-xylose
-
?
UDP-D-xylose + zeatin
UDP + O-beta-D-xylosylzeatin
show the reaction diagram
-
trans-zeatin
-
?
UDP-D-xylose + zeatin
UDP + O-beta-D-xylosylzeatin
show the reaction diagram
Phaseolus vulgaris, Phaseolus vulgaris x Phaseolus coccineus
-
trans-zeatin
-
?
UDP-D-xylose + zeatin
UDP + O-beta-D-xylosylzeatin
show the reaction diagram
-
cis-zeatin and ribosylzeatin are no substrates
-
?
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
(Substrate)
LITERATURE
(Substrate)
COMMENTARY
(Product)
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
UDP-D-xylose + trans-zeatin
?
show the reaction diagram
Phaseolus vulgaris, Phaseolus vulgaris x Phaseolus coccineus
-
may be involved in the nuclear-cytoplasmic transport of cytokinins and related molecules or possibly, with chromatin of rapidly dividing cells
-
-
-
INHIBITORS
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
IMAGE
KM VALUE [mM]
KM VALUE [mM] Maximum
SUBSTRATE
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
IMAGE
0.01
-
dihydrozeatin
-
-
0.002
-
trans-zeatin
-
-
0.003
-
UDP-xylose
-
-
SPECIFIC ACTIVITY [µmol/min/mg]
SPECIFIC ACTIVITY MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
0.0049
-
-
-
pH OPTIMUM
pH MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
8
-
-
assay at
TEMPERATURE OPTIMUM
TEMPERATURE OPTIMUM MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
25
-
-
assay at
27
-
-
assay at
27
-
P56725
assay at
SOURCE TISSUE
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
SOURCE
Phaseolus vulgaris, Phaseolus vulgaris x Phaseolus coccineus
-
-
Manually annotated by BRENDA team
Phaseolus vulgaris x Phaseolus coccineus
-
callus
Manually annotated by BRENDA team
additional information
Phaseolus vulgaris, Phaseolus vulgaris x Phaseolus coccineus
-
no activity in vegetative tissues, activity is apparent only in reproductive tissue, endosperm callus represents a unique form inbetween
Manually annotated by BRENDA team
additional information
P56725
no activity in vegetative tissues, activity is apparent only in reproductive tissue, endosperm callus represents a unique form inbetween; very low expression level in vegetative tissues
Manually annotated by BRENDA team
LOCALIZATION
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
GeneOntology No.
LITERATURE
SOURCE
Phaseolus vulgaris x Phaseolus coccineus
-
-
Manually annotated by BRENDA team
Phaseolus vulgaris x Phaseolus coccineus
-
-
Manually annotated by BRENDA team
MOLECULAR WEIGHT
MOLECULAR WEIGHT MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
50000
-
-
gel filtration
51000
-
P56725
DNA sequence determination
additional information
-
-
an inactive form of MW about 70 kDa is detected by immunostaining in vegetative tissue prior to posttranslational processing of the protein
SUBUNITS
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
monomer
-
1 * 50000, SDS-PAGE
Purification/COMMENTARY
ORGANISM
UNIPROT ACCESSION NO.
LITERATURE
Cloned/COMMENTARY
ORGANISM
UNIPROT ACCESSION NO.
LITERATURE
expression in Escherichia coli and in Spodoptera frugiperda Sf21 cells, via baculovirus infection, produced an insoluble protein, not successful; in vitro transcription and translation, posttranslational processing by bean endosperm extract
-
ZOX1 gene, DNA sequence determination and analysis
P56725
ENGINEERING
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
additional information
-
construction of chimeric recombinant enzyme mutants, exchange of the C-terminus with zeatin O-beta-D-glucosyltransferase, EC 2.4.1.203, gene ZOG1, determination of the site determining the substrate specificity
additional information
-
construction of transgenic tobacco plants via Agrobacterium tumefaciens infection, functional expression under control of the CaMV35S promotor in seedlings and leaves, transgenic plants are more sensitive to auxin than wild-type plants, phenotypic alterations are leaf chlorosis, restriction of root elongation and eventual cessation of growth