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expression in Escherichia coli and in Spodoptera frugiperda Sf21 cells, via baculovirus infection, produced an insoluble protein, not successful; in vitro transcription and translation, posttranslational processing by bean endosperm extract
construction of chimeric recombinant enzyme mutants, exchange of the C-terminus with zeatin O-beta-D-glucosyltransferase, EC 126.96.36.199, gene ZOG1, determination of the site determining the substrate specificity
construction of transgenic tobacco plants via Agrobacterium tumefaciens infection, functional expression under control of the CaMV35S promotor in seedlings and leaves, transgenic plants are more sensitive to auxin than wild-type plants, phenotypic alterations are leaf chlorosis, restriction of root elongation and eventual cessation of growth