Information on EC 2.4.2.39 - xyloglucan 6-xylosyltransferase

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The expected taxonomic range for this enzyme is: rosids

EC NUMBER
COMMENTARY
2.4.2.39
-
RECOMMENDED NAME
GeneOntology No.
xyloglucan 6-xylosyltransferase
REACTION
REACTION DIAGRAM
COMMENTARY
ORGANISM
UNIPROT
LITERATURE
Transfers an alpha-D-xylosyl residue from UDP-D-xylose to a glucose residue in xyloglucan, forming an alpha-(1->6)-D-xylosyl-D-glucose linkage
show the reaction diagram
-
-
-
-
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
hexosyl group transfer
-
-
-
-
hexosyl group transfer
-
xyloglucan alpha-(1,6)-xylosyltransferase activity, cell wall xyloglucan biosynthesis
PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
xyloglucan biosynthesis
-
-
SYSTEMATIC NAME
IUBMB Comments
UDP-D-xylose:xyloglucan 6-alpha-D-xylosyltransferase
In association with EC 2.4.1.168 (xyloglucan 4-glucosyltransferase), this enzyme brings about the synthesis of xyloglucan; concurrent transfers of glucose and xylose are necessary for this synthesis.
SYNONYMS
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
At1g74380
Q9CA75
-
EC2.4.1.169
-
-
formerly
-
XXT5
Q9CA75
putative xyloglycan xylosylransferase
xyloglucan 6-alpha-D-xylosyltransferase
-
-
-
-
xyloglucan alpha-1,6-xylosyltransferase
O22775, Q9CA75, Q9LJP4, Q9LZJ3
-
xylosyltransferase, uridine diphosphoxylose-xyloglucan 6alpha-
-
-
-
-
CAS REGISTRY NUMBER
COMMENTARY
80238-01-3
-
ORGANISM
COMMENTARY
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
soy bean
-
-
Manually annotated by BRENDA team
dwarf-french-bean, cv. Canadian Wonder
-
-
Manually annotated by BRENDA team
pea, cv. Alaska or Caprice
-
-
Manually annotated by BRENDA team
tamarind
-
-
Manually annotated by BRENDA team
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
malfunction
-
double mutants for either xxt2 or xxt5 have a large impact on XyG content, structure and size distribution
malfunction
Q9LJP4
overexpression of XXT3, is able to restore XyG epitopes in xxt2, xxt5 and xxt2 xxt5 double knockouts, suggesting that it also encodes a protein with XXT activity
SUBSTRATE
PRODUCT                      
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
LITERATURE
(Substrate)
COMMENTARY
(Product)
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
UDP-D-xylose + (glucosyl)xyloglucan
UDP + (xylosyl-glucosyl)xyloglucan
show the reaction diagram
O22775, Q9LJP4, Q9LZJ3
-
-
-
?
UDP-D-xylose + (glucosyl)xyloglucan
UDP + (xylosyl-glucosyl)xyloglucan
show the reaction diagram
Q9CA75
-
reduction of xyloglucan content (reduced glucan backbone substitution with xylose) in plants that lack functional XXT5 can be complemented by transformation with recombinant XXT5
-
?
UDP-D-xylose + (glucosyl)xyloglucan
UDP + (xylosyl-glucosyl)xyloglucan
show the reaction diagram
-
standard oligosaccharides for enzyme assay
-
-
?
UDP-D-xylose + (glucosyl)xyloglucan
UDP + (xylosyl-glucosyl)xyloglucan
show the reaction diagram
-
xylosyl-transfer is closely linked to glucosyl-transfer (EC 2.4.1.168)
-
?
UDP-D-xylose + (glucosyl)xyloglucan
UDP + (xylosyl-glucosyl)xyloglucan
show the reaction diagram
-
GDP-D-glucose or GDP-D-mannose cannot replace UDPxylose
-
?
UDP-D-xylose + (glucosyl)xyloglucan
UDP + (xylosyl-glucosyl)xyloglucan
show the reaction diagram
-
no acceptors are cello-oligosaccharides and fragment oligosaccharides from endoglucanase digest, other xylosyl-acceptors are beta-1,3-glucan and xylan
forms an alpha-1,6-D-xylosyl-D-glucose linkage
?
UDP-D-xylose + (glucosyl)xyloglucan
UDP + (xylosyl-glucosyl)xyloglucan
show the reaction diagram
-
transfers an alpha-D-xylosyl residue from UDP-D-xylose to a glucose residue in xyloglucan
-
?
UDP-D-xylose + (glucosyl)xyloglucan
UDP + (xylosyl-glucosyl)xyloglucan
show the reaction diagram
-
transfers an alpha-D-xylosyl residue from UDP-D-xylose to a glucose residue in xyloglucan
-
?
UDP-D-xylose + (glucosyl)xyloglucan
UDP + (xylosyl-glucosyl)xyloglucan
show the reaction diagram
-
transfers an alpha-D-xylosyl residue from UDP-D-xylose to a glucose residue in xyloglucan
-
?
UDP-D-xylose + (glucosyl)xyloglucan
UDP + (xylosyl-glucosyl)xyloglucan
show the reaction diagram
-
transfers an alpha-D-xylosyl residue from UDP-D-xylose to a glucose residue in xyloglucan
forms an alpha-1,6-D-xylosyl-D-glucose linkage
?
UDP-D-xylose + xyloglucan
?
show the reaction diagram
-
responsible for xyloglucan side-chain formation
-
-
?
UDP-D-xylose + xyloglucan
?
show the reaction diagram
-
involved in xyloglucan biosynthesis of higher plants
-
-
?
additional information
?
-
Q9CA75
no xylosyltransferase activity in vitro after expression in either Pichia pastoris or Sf21 insect cells using UDP-xylose and a broad range of putative acceptors
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-
-
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
LITERATURE
(Substrate)
COMMENTARY
(Product)
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
UDP-D-xylose + (glucosyl)xyloglucan
UDP + (xylosyl-glucosyl)xyloglucan
show the reaction diagram
Q9CA75
-
reduction of xyloglucan content (reduced glucan backbone substitution with xylose) in plants that lack functional XXT5 can be complemented by transformation with recombinant XXT5
-
?
UDP-D-xylose + xyloglucan
?
show the reaction diagram
-
responsible for xyloglucan side-chain formation
-
-
?
UDP-D-xylose + xyloglucan
?
show the reaction diagram
-
involved in xyloglucan biosynthesis of higher plants
-
-
?
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
Ca2+
-
activation, can replace Mn2+ to some extent
Co2+
-
activation, can replace Mn2+ to some extent
Mg2+
-
activation, can replace Mn2+ to some extent
Mg2+
-
activation, can replace Mn2+ to some extent
Mn2+
-
10 mM; activation
Mn2+
-
activation
Mn2+
-
activation
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
Detergents
-
no solubilization possible due to this inhibition
EDTA
-
weak
Phosphate buffer
-
-
-
Tris-HCl buffer
-
-
GDPglucose
-
weak
additional information
-
no inhibition by tunicamycin, ATP or GTP
-
ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
GDP-D-glucose
-
about half as effective as UDPglucose, in concentrations exceeding UDPxylose; activation
GDP-D-glucose
-
activation; protection
GDP-D-mannose
-
activation, protection
TDPglucose
-
activation, as effective as UDPglucose, in concentrations exceeding UDPxylose
UDPglucose
-
-
UDPglucose
-
activation, xylose is effectively incorporated in the presence of UDPglucose, the transfer must be preceded by elongation of the beta-1,4-glucan-backbone, because xylosyl residues constitute the side chains, in concentrations exceeding UDPxylose, no activation by CDPglucose or ADPglucose
UDPglucose
-
2 mM
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
additional information
additional information
-
kinetic study
-
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
6
-
incorporation of xylosyl residues into polymeric acceptors
6.5 - 7
-
UDPglucose + UDPxylose
6.9
Q9LJP4
assay at; assay at; assay at; assay at
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
30
Q9LJP4
assay at; assay at; assay at; assay at
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
SOURCE
-
high expression level, revealed by Reverse Transcription-PCR
Manually annotated by BRENDA team
-
elongation region
Manually annotated by BRENDA team
-
expressed in all major organs, revealed by Reverse Transcription-PCR
Manually annotated by BRENDA team
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high expression level, revealed by Reverse Transcription-PCR, shorter root hair and less root tissue xyloglucan detectable upon lack of XXT5
Manually annotated by BRENDA team
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silique, lowest expression level, revealed by Reverse Transcription-PCR
Manually annotated by BRENDA team
-
high expression level, revealed by Reverse Transcription-PCR
Manually annotated by BRENDA team
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY
GeneOntology No.
LITERATURE
SOURCE
-
immunolocalization of recombinant enzyme using tag-specific antibody, predicted Golgi-resident type-II membrane protein
Manually annotated by BRENDA team
-
complex formation in vivo and potential physical interactions among three xylosyltransferases, XXT1, XXT2, and XXT5, and a glucan synthase, CSLC4 is shown in the Golgi membrane in Arabidopsis cells
Manually annotated by BRENDA team
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
51000
-
predicted from primary structure
694682
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
heterodimer
-
existence of heterocomplex XXT1-XXT2 is demonstrated, existence of heterocomplexes XXT2-XXT5 and XXT1-XXT2 are demonstrated, existence of heterocomplexes XXT2-XXT5 and XXT5-CSLC4 are demonstrated
homodimer
-
existence of homocomplex XXT2-XXT2 is shown
GENERAL STABILITY
ORGANISM
UNIPROT
LITERATURE
DTT, 1 mM, EDTA, 1 mM, sucrose, 0.4 M, bovine serum albumin, 0.1%, stabilize
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STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
0°C, crude membrane-bound enzyme preparation, 1 day
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Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
expressed in Escherichia coli as a truncated protein lacking the N-terminal transmembrane domain as GST-fusion protein; expressed in Escherichia coli as a truncated protein lacking the N-terminal transmembrane domain as GST-fusion protein; expressed in Escherichia coli as a truncated protein lacking the N-terminal transmembrane domain as GST-fusion protein; expressed in Escherichia coli as a truncated protein lacking the N-terminal transmembrane domain as GST-fusion protein
Q9LJP4
in pENTR/D-TOPO downstream of cauliflower mosaic virus 35S promoter for recombination with pEarleyGate 201 and Agrobacterium tumefacies strain GV3101-mediated transformation (floral-flip), expression with hemagglutinin (HA) tag
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