Information on EC 2.4.2.35 - flavonol-3-O-glycoside xylosyltransferase

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The expected taxonomic range for this enzyme is: Tulipa hybrid cultivar

EC NUMBER
COMMENTARY
2.4.2.35
-
RECOMMENDED NAME
GeneOntology No.
flavonol-3-O-glycoside xylosyltransferase
REACTION
REACTION DIAGRAM
COMMENTARY
ORGANISM
UNIPROT ACCESSION NO.
LITERATURE
UDP-alpha-D-xylose + a flavonol 3-O-glycoside = UDP + a flavonol 3-[beta-D-xylosyl-(1->2)-beta-D-glycoside]
show the reaction diagram
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-
-
-
REACTION TYPE
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
pentosyl group transfer
-
-
-
-
PATHWAY
KEGG Link
MetaCyc Link
Flavone and flavonol biosynthesis
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SYSTEMATIC NAME
IUBMB Comments
UDP-alpha-D-xylose:flavonol-3-O-glycoside 2''-O-beta-D-xylosyltransferase
Flavonol 3-O-glucoside, flavonol 3-O-galactoside and, more slowly, rutin, can act as acceptors.
SYNONYMS
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
UDP-xylose:flavonol 3-glycoside xylosyltransferase
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-
-
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uridine diphosphoxylose-flavonol 3-glycoside
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-
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xylosyltransferase
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-
-
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CAS REGISTRY NUMBER
COMMENTARY
83380-90-9
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ORGANISM
COMMENTARY
LITERATURE
SEQUENCE CODE
SEQUENCE DB
SOURCE
tulip, cv. Apeldoorn
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-
Manually annotated by BRENDA team
SUBSTRATE
PRODUCT                      
REACTION DIAGRAM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
(Substrate)
LITERATURE
(Substrate)
COMMENTARY
(Product)
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
UDP-D-xylose + flavonol 3-O-diglycoside
UDP + flavonol 3-O-triglycoside
show the reaction diagram
-
-
-
-
?
UDP-D-xylose + flavonol 3-O-glycoside
UDP + flavonol 3-O-D-xylosylglycoside
show the reaction diagram
-
-
-
?
UDP-D-xylose + flavonol 3-O-glycoside
UDP + flavonol 3-O-D-xylosylglycoside
show the reaction diagram
-
involved in flavonoid metabolism, pathway of flavonol 3-O-triglycoside biosynthesis
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-
-
UDP-D-xylose + isorhamnetin 3,7-O-diglucoside
?
show the reaction diagram
-
poor substrate
-
-
?
UDP-D-xylose + isorhamnetin 3-O-glucoside
UDP + isorhamnetin 3-O-xylosylglucoside
show the reaction diagram
-
-
-
-
?
UDP-D-xylose + kaempferol 3-O-glucoside
UDP + kaempferol 3-O-xylosylglucoside
show the reaction diagram
-
-
-
-
?
UDP-D-xylose + kaempferol 3-O-rhamnoside
UDP + kaempferol 3-O-xylosylrhamnoside
show the reaction diagram
-
poor substrate
-
-
?
UDP-D-xylose + myricetin 3-O-rhamnoside
UDP + myricetin 3-O-xylosylrhamnoside
show the reaction diagram
-
poor substrate
-
-
?
UDP-D-xylose + quercetin 3-O-arabinoglucoside
UDP + quercetin 3-O-xylosylarabinoglucoside
show the reaction diagram
-
poor substrate
-
-
?
UDP-D-xylose + quercetin 3-O-galactoside
UDP + quercetin 3-O-xylosylgalactoside
show the reaction diagram
-
best substrate tested
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-
?
UDP-D-xylose + quercetin 3-O-glucoside
UDP + quercetin 3-O-xylosylglucoside
show the reaction diagram
-
-
-
-
?
UDP-D-xylose + quercetin 3-O-glucoside
UDP + quercetin 3-O-D-xylosylglucoside
show the reaction diagram
-
poor substrate
-
-
?
UDP-D-xylose + quercetin 3-O-rhamnoside
UDP + quercetin 3-O-xylosylrhamnoside
show the reaction diagram
-
poor substrate
-
?
UDP-D-xylose + quercetin 3-O-rhamnosylglucoside
UDP + quercetin 3-O-D-xylosylrhamnosylglucoside
show the reaction diagram
-
i.e. rutin, poor substrate
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?
additional information
?
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-
aglycones are no substrates
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NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
(Substrate)
LITERATURE
(Substrate)
COMMENTARY
(Product)
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
UDP-D-xylose + flavonol 3-O-glycoside
UDP + flavonol 3-O-D-xylosylglycoside
show the reaction diagram
-
involved in flavonoid metabolism, pathway of flavonol 3-O-triglycoside biosynthesis
-
-
-
METALS and IONS
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
Ca2+
-
high activation
additional information
-
no effect by Mg2+
INHIBITORS
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
IMAGE
Cu2+
-
high inhibition when applied in high concentrations
p-chloromercuribenzoate
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-
Zn2+
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high inhibition when applied in high concentrations
ACTIVATING COMPOUND
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
IMAGE
2-mercaptoethanol
-
slight stimulation
dithioerythritol
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slight stimulation
NH4+
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high activation
Sucrose
-
slight stimulation
glutathione
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slight stimulation
additional information
-
no stimulation by bovine serum albumin, not affected by anions like chloride and sulfate
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pH OPTIMUM
pH MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
SOURCE TISSUE
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
SOURCE
-
at the stage of middle postmeiotic pollen ripening
Manually annotated by BRENDA team
MOLECULAR WEIGHT
MOLECULAR WEIGHT MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
30000
-
-
gel filtration
Purification/COMMENTARY
ORGANISM
UNIPROT ACCESSION NO.
LITERATURE
partial by a method that includes Sephadex G-200 chromatography, Sephadex G-15 chromatography, isoelectric focusing and DEAE-Sephadex chromatography
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