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Information on EC 2.4.2.29 - tRNA-guanosine34 preQ1 transglycosylase and Organism(s) Homo sapiens and UniProt Accession Q9BXR0
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2.4.2.29 tRNA-guanosine34 preQ1 transglycosylaseIUBMB Comments
Certain prokaryotic and eukaryotic tRNAs contain the modified base queuine at position 34. In eubacteria, which produce queuine de novo, the enzyme catalyses the exchange of guanine with the queuine precursor preQ1, which is ultimately modified to queuosine . The enzyme can also use an earlier intermediate, preQ0, to replace guanine in unmodified tRNATyr and tRNAAsn . This enzyme acts after EC 1.7.1.13, preQ1 synthase, in the queuine-biosynthesis pathway. cf. EC 2.4.2.64, tRNA-guanosine34 queuine transglycosylase.
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Word Map
- 2.4.2.29
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anticodon
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queuosine
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wobble
- mobilis
- trnatyr
- shigella
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zymomonas
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hypermodified
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archaeosine
- shigellosis
- preq0
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trna-modifying
- trnaasn
- trnahis
- 7-cyano-7-deazaguanine
- minihelix
- 7-deazaguanosine
- garcia
- medicine
The taxonomic range for the selected organisms is: Homo sapiens
The expected taxonomic range for this enzyme is: Bacteria, Archaea, Eukaryota
The expected taxonomic range for this enzyme is: Bacteria, Archaea, Eukaryota
Reaction Schemes
+
=
7-aminomethyl-7-carbaguanine34 in tRNA
+
Synonyms
trna-guanine transglycosylase, arctgt, qtrt1, trna guanine transglycosylase, trna transglycosylase, guanine insertion enzyme, queuine trna-ribosyltransferase, trna-guanine 34 transglycosylase, q-insertase, queuine trna ribosyltransferase, 
more
topSYNONYM 
ORGANISM
UNIPROT
COMMENTARY 
LITERATURE
guanine insertion enzyme
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-
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guanine, queuine-tRNA transglycosylase
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-
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Q-insertase
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-
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queuine insertase
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-
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queuine tRNA ribosyltransferase
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-
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queuine tRNA-ribosyltransferase
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-
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ribosyltransferase, queuine transfer ribonucleate
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TGT
transfer ribonucleate glycosyltransferase
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-
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tRNA guanine transglycosidase
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-
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tRNA guanine transglycosylase
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-
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tRNA transglycosylase
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-
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tRNA-guanine transglycosylase
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-
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virulence-associated protein VACC
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-
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TGT
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-
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-
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
pentosyl group transfer
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-
-
-
PATHWAY SOURCE
PATHWAYS
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-, -
SYSTEMATIC NAME
IUBMB Comments
tRNA-guanosine34:7-aminomethyl-7-deazaguanine tRNA-D-ribosyltransferase
Certain prokaryotic and eukaryotic tRNAs contain the modified base queuine at position 34. In eubacteria, which produce queuine de novo, the enzyme catalyses the exchange of guanine with the queuine precursor preQ1, which is ultimately modified to queuosine [5]. The enzyme can also use an earlier intermediate, preQ0, to replace guanine in unmodified tRNATyr and tRNAAsn [1]. This enzyme acts after EC 1.7.1.13, preQ1 synthase, in the queuine-biosynthesis pathway. cf. EC 2.4.2.64, tRNA-guanosine34 queuine transglycosylase.
CAS REGISTRY NUMBER
COMMENTARY
72162-89-1
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SUBSTRATE
PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
(Substrate)
LITERATURE
(Substrate)
(Substrate)
COMMENTARY
(Product)
(Product)
LITERATURE
(Product)
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
additional information
?
-
-
higher expression of the subunit TGT60KD in cancer cells than in normal cells. The expression levels of the TGT60KD subunit regulate enzyme activity and the level of queuosine. TGT60KD plays significant roles in carcinogenesis
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-
?
additional information
?
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the 60000 Da subunit of the enzyme plays a role in colon carcinogenesis
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-
?
NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
(Substrate)
LITERATURE
(Substrate)
(Substrate)
COMMENTARY
(Product)
(Product)
LITERATURE
(Product)
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
additional information
?
-
-
higher expression of the subunit TGT60KD in cancer cells than in normal cells. The expression levels of the TGT60KD subunit regulate enzyme activity and the level of queuosine. TGT60KD plays significant roles in carcinogenesis
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-
?
additional information
?
-
-
the 60000 Da subunit of the enzyme plays a role in colon carcinogenesis
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-
?
ORGANISM
COMMENTARY
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
SOURCE
-
the expression level of the 60000 Da subunit is elevated in four of five colon cancer cell lines and 83% of colon cancer tissue compared with normal tissue
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY
GeneOntology No.
LITERATURE
SOURCE
UNIPROT
ENTRY NAME
ORGANISM
NO. OF AA
NO. OF TRANSM. HELICES
MOLECULAR WEIGHT[Da]
SOURCE
SEQUENCE
LOCALIZATION PREDICTION
The reliability class of the localization prediction ranges from 1 (very reliable) to 5 (not reliable).
The reliability class of the localization prediction ranges from 1 (very reliable) to 5 (not reliable). TGT_HUMAN
403
0
44048
Swiss-Prot
other Location (Reliability: 4)
PDB
SCOP
CATH
UNIPROT
ORGANISM
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
additional information
-
sequence identities between prokaryotic, eukaryotic and archaebacterial enzymes
SUBUNIT
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
CRYSTALLIZATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
apoenzyme and in complex with queuine, sitting or hanging drop vapor diffusion method, using 100 mM Tris pH 7.8, 200 mM KBr, 200 mM KSCN, 3% (w/v) polyglutamic acid-LM, 5% (w/v) PEG 4000
PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Romier, C.; Meyer, J.E.W.; Suck, D.
Slight sequence variations of a common fold explain the substrate specificities of tRNA-guanine transglycosylases from the three kingdoms
FEBS Lett.
416
93-98
1997
Caenorhabditis elegans, Haemophilus influenzae, Helicobacter pylori, Homo sapiens, Mus musculus, Shigella flexneri, Synechocystis sp., Thermotoga maritima
Ishiwata, S.; Katayama, J.; Shindo, H.; Ozawa, Y.; Itoh, K.; Mizugaki, M.
Increased expression of queuosine synthesizing enzyme, tRNA-guanine transglycosylase, and queuosine levels in tRNA of leukemic cells
J. Biochem.
129
13-17
2001
Homo sapiens
Ishiwata, S.; Ozawa, Y.; Katayama, J.; Kaneko, S.; Shindo, H.; Tomioka, Y.; Ishiwata, T.; Asano, G.; Ikegawa, S.; Mizugaki, M.
Elevated expression level of 60-kDa subunit of tRNA-guanine transglycosylase in colon cancer
Cancer Lett.
212
113-119
2004
Homo sapiens
Stengl, B.; Reuter, K.; Klebe, G.
Mechanism and substrate specificity of tRNA-guanine transglycosylases (TGTs): tRNA-modifying enzymes from the three different kingdoms of life share a common catalytic mechanism
Chembiochem
6
1926-1939
2005
Escherichia coli, Homo sapiens, Schizosaccharomyces pombe, Zymomonas mobilis (P28720)
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