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EC Tree
IUBMB Comments Certain prokaryotic and eukaryotic tRNAs contain the modified base queuine at position 34. In eubacteria, which produce queuine de novo, the enzyme catalyses the exchange of guanine with the queuine precursor preQ1, which is ultimately modified to queuosine . The enzyme can also use an earlier intermediate, preQ0, to replace guanine in unmodified tRNATyr and tRNAAsn . This enzyme acts after EC 1.7.1.13, preQ1 synthase, in the queuine-biosynthesis pathway. cf. EC 2.4.2.64, tRNA-guanosine34 queuine transglycosylase.
The taxonomic range for the selected organisms is: Homo sapiens The expected taxonomic range for this enzyme is: Bacteria, Archaea, Eukaryota
Reaction Schemes
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7-aminomethyl-7-carbaguanine34 in tRNA
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Synonyms
trna-guanine transglycosylase, arctgt, qtrt1, trna guanine transglycosylase, trna transglycosylase, guanine insertion enzyme, queuine trna-ribosyltransferase, trna-guanine 34 transglycosylase, q-insertase, queuine trna ribosyltransferase,
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queuine tRNA-ribosyltransferase subunit 1
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tRNA guanine transglycosylase
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guanine insertion enzyme
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guanine, queuine-tRNA transglycosylase
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queuine insertase
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queuine tRNA ribosyltransferase
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queuine tRNA-ribosyltransferase
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ribosyltransferase, queuine transfer ribonucleate
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transfer ribonucleate glycosyltransferase
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tRNA guanine transglycosidase
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tRNA guanine transglycosylase
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tRNA transglycosylase
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tRNA-guanine transglycosylase
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virulence-associated protein VACC
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TGT
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pentosyl group transfer
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tRNA-guanosine34:7-aminomethyl-7-deazaguanine tRNA-D-ribosyltransferase
Certain prokaryotic and eukaryotic tRNAs contain the modified base queuine at position 34. In eubacteria, which produce queuine de novo, the enzyme catalyses the exchange of guanine with the queuine precursor preQ1, which is ultimately modified to queuosine [5]. The enzyme can also use an earlier intermediate, preQ0, to replace guanine in unmodified tRNATyr and tRNAAsn [1]. This enzyme acts after EC 1.7.1.13, preQ1 synthase, in the queuine-biosynthesis pathway. cf. EC 2.4.2.64, tRNA-guanosine34 queuine transglycosylase.
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guanine34 in tRNA + queuine
queuine34 in tRNA + guanine
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additional information
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additional information
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higher expression of the subunit TGT60KD in cancer cells than in normal cells. The expression levels of the TGT60KD subunit regulate enzyme activity and the level of queuosine. TGT60KD plays significant roles in carcinogenesis
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additional information
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the 60000 Da subunit of the enzyme plays a role in colon carcinogenesis
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guanine34 in tRNA + queuine
queuine34 in tRNA + guanine
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additional information
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additional information
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higher expression of the subunit TGT60KD in cancer cells than in normal cells. The expression levels of the TGT60KD subunit regulate enzyme activity and the level of queuosine. TGT60KD plays significant roles in carcinogenesis
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additional information
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the 60000 Da subunit of the enzyme plays a role in colon carcinogenesis
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Breast Neoplasms
tRNA Queuosine Modification Enzyme Modulates the Growth and Microbiome Recruitment to Breast Tumors.
Carcinogenesis
tRNA Queuosine Modification Enzyme Modulates the Growth and Microbiome Recruitment to Breast Tumors.
Colonic Neoplasms
Absence of tRNA-guanine transglycosylase in a human colon adenocarcinoma cell line.
Colonic Neoplasms
Elevated expression level of 60-kDa subunit of tRNA-guanine transglycosylase in colon cancer.
Dysentery, Bacillary
A new target for shigellosis: rational design and crystallographic studies of inhibitors of tRNA-guanine transglycosylase.
Dysentery, Bacillary
An Immucillin-based Transition State-Analogous Inhibitor of tRNA-Guanine Transglycosylase (TGT).
Dysentery, Bacillary
Crystal structure analysis and in silico pKa calculations suggest strong pKa shifts of ligands as driving force for high-affinity binding to TGT.
Dysentery, Bacillary
Fragment Screening Hit Draws Attention to a Novel Transient Pocket Adjacent to the Recognition Site of the tRNA-Modifying Enzyme TGT.
Dysentery, Bacillary
High-Affinity Inhibitors of tRNA-Guanine Transglycosylase Replacing the Function of a Structural Water Cluster.
Dysentery, Bacillary
High-affinity inhibitors of Zymomonas mobilis tRNA-guanine transglycosylase through convergent optimization.
Dysentery, Bacillary
How to replace the residual solvation shell of polar active site residues to achieve nanomolar inhibition of tRNA-guanine transglycosylase.
Dysentery, Bacillary
Launching Spiking Ligands into a Protein-Protein Interface: A Promising Strategy to Destabilize and Break Interface Formation in a tRNA Modifying Enzyme.
Dysentery, Bacillary
Replacement of water molecules in a phosphate binding site by furanoside-appended lin-benzoguanine ligands of tRNA-guanine transglycosylase (TGT).
Dysentery, Bacillary
Soaking suggests "alternative facts": Only co-crystallization discloses major ligand-induced interface rearrangements of a homodimeric tRNA-binding protein indicating a novel mode-of-inhibition.
Dysentery, Bacillary
Structure-based drug design: exploring the proper filling of apolar pockets at enzyme active sites.
Dysentery, Bacillary
Swapping Interface Contacts in the Homodimeric tRNA-Guanine Transglycosylase: An Option for Functional Regulation.
Dysentery, Bacillary
Targeting a Cryptic Pocket in a Protein-Protein Contact by Disulfide-Induced Rupture of a Homodimeric Interface.
Dysentery, Bacillary
Unraveling a Ligand-Induced Twist of a Homodimeric Enzyme by Pulsed Electron-Electron Double Resonance.
Foodborne Diseases
High-affinity inhibitors of Zymomonas mobilis tRNA-guanine transglycosylase through convergent optimization.
Foodborne Diseases
Replacement of water molecules in a phosphate binding site by furanoside-appended lin-benzoguanine ligands of tRNA-guanine transglycosylase (TGT).
Liver Neoplasms, Experimental
Detection of unique tRNA species in tumor tissues by Escherichia coli guanine insertion enzyme.
Multiple Sclerosis
The human tRNA-guanine transglycosylase displays promiscuous nucleobase preference but strict tRNA specificity.
Neoplasms
Characterization and analysis of oncofetal tRNA and its possible application for cancer diagnosis and therapy.
Neoplasms
Detection of unique tRNA species in tumor tissues by Escherichia coli guanine insertion enzyme.
Neoplasms
tRNA Queuosine Modification Enzyme Modulates the Growth and Microbiome Recruitment to Breast Tumors.
trna-guanosine34 preq1 transglycosylase
deficiency
tRNA Queuosine Modification Enzyme Modulates the Growth and Microbiome Recruitment to Breast Tumors.
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UniProt
brenda
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the expression level of the 60000 Da subunit is elevated in four of five colon cancer cell lines and 83% of colon cancer tissue compared with normal tissue
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normal
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TGT_HUMAN
403
0
44048
Swiss-Prot
other Location (Reliability: 4 )
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additional information
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sequence identities between prokaryotic, eukaryotic and archaebacterial enzymes
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apoenzyme and in complex with queuine, sitting or hanging drop vapor diffusion method, using 100 mM Tris pH 7.8, 200 mM KBr, 200 mM KSCN, 3% (w/v) polyglutamic acid-LM, 5% (w/v) PEG 4000
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His-Talon column chromatography and Superdex S200 gel filtration
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expressed in Escherichia coli BL21(DE3)-STAR cells
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Romier, C.; Meyer, J.E.W.; Suck, D.
Slight sequence variations of a common fold explain the substrate specificities of tRNA-guanine transglycosylases from the three kingdoms
FEBS Lett.
416
93-98
1997
Caenorhabditis elegans, Haemophilus influenzae, Helicobacter pylori, Homo sapiens, Mus musculus, Shigella flexneri, Synechocystis sp., Thermotoga maritima
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Ishiwata, S.; Katayama, J.; Shindo, H.; Ozawa, Y.; Itoh, K.; Mizugaki, M.
Increased expression of queuosine synthesizing enzyme, tRNA-guanine transglycosylase, and queuosine levels in tRNA of leukemic cells
J. Biochem.
129
13-17
2001
Homo sapiens
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Ishiwata, S.; Ozawa, Y.; Katayama, J.; Kaneko, S.; Shindo, H.; Tomioka, Y.; Ishiwata, T.; Asano, G.; Ikegawa, S.; Mizugaki, M.
Elevated expression level of 60-kDa subunit of tRNA-guanine transglycosylase in colon cancer
Cancer Lett.
212
113-119
2004
Homo sapiens
brenda
Stengl, B.; Reuter, K.; Klebe, G.
Mechanism and substrate specificity of tRNA-guanine transglycosylases (TGTs): tRNA-modifying enzymes from the three different kingdoms of life share a common catalytic mechanism
Chembiochem
6
1926-1939
2005
Escherichia coli, Homo sapiens, Schizosaccharomyces pombe, Zymomonas mobilis (P28720)
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Johannsson, S.; Neumann, P.; Ficner, R.
Crystal structure of the human tRNA guanine transglycosylase catalytic subunit QTRT1
Biomolecules
8
81
2018
Homo sapiens (Q9BXR0), Homo sapiens
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