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Information on EC 2.4.2.28 - S-methyl-5'-thioadenosine phosphorylase and Organism(s) Mus musculus and UniProt Accession Q9CQ65

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     2 Transferases
         2.4 Glycosyltransferases
             2.4.2 Pentosyltransferases
                2.4.2.28 S-methyl-5'-thioadenosine phosphorylase
IUBMB Comments
Also acts on 5'-deoxyadenosine and other analogues having 5'-deoxy groups.
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This record set is specific for:
Mus musculus
UNIPROT: Q9CQ65
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The taxonomic range for the selected organisms is: Mus musculus
The expected taxonomic range for this enzyme is: Bacteria, Eukaryota, Archaea
Synonyms
methylthioadenosine phosphorylase, 5'-methylthioadenosine phosphorylase, mta phosphorylase, mtap protein, 5'-deoxy-5'-methylthioadenosine phosphorylase, ssmtapii, ssmtap, mtapase, mesado phosphorylase, pfmtap, more
SYNONYM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
5'-deoxy-5'-methylthioadenosine:orthophosphate methylthioribosyltransferase
-
5'-methylthioadenosine phosphorylase
-
5'-deoxy-5'-methylthioadenosine phosphorylase
-
-
-
-
5'-methylthioadenosine nucleosidase
-
-
-
-
5'-methylthioadenosine phosphorylase
-
-
-
-
MeSAdo phosphorylase
-
-
-
-
MeSAdo/Ado phosphorylase
-
-
-
-
methylthioadenosine nucleoside phosphorylase
-
-
-
-
methylthioadenosine phosphorylase
MTA phosphorylase
-
-
-
-
MTAP
-
-
MTAPase
-
-
-
-
phosphorylase, methylthioadenosine
-
-
-
-
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
pentosyl group transfer
-
-
-
-
PATHWAY SOURCE
PATHWAYS
-
-, -
SYSTEMATIC NAME
IUBMB Comments
S-methyl-5'-thioadenosine:phosphate S-methyl-5-thio-alpha-D-ribosyl-transferase
Also acts on 5'-deoxyadenosine and other analogues having 5'-deoxy groups.
CAS REGISTRY NUMBER
COMMENTARY hide
61970-06-7
-
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
5'-methylthioadenosine + phosphate
adenine + 5-methylthio-D-ribose 1-phosphate
show the reaction diagram
-
-
-
r
5'-deoxy-5'-methylthioadenosine + phosphate
adenine + 5-methylthio-D-ribose 1-phosphate
show the reaction diagram
-
-
-
-
r
5'-deoxyadenosine + phosphate
adenine + 5-deoxy-D-ribose 1-phosphate
show the reaction diagram
-
phosphate-dependent
-
r
5'-methylthioadenosine + phosphate
adenine + 5-methylthio-D-ribose 1-phosphate
show the reaction diagram
NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
5'-methylthioadenosine + phosphate
adenine + 5-methylthio-D-ribose 1-phosphate
show the reaction diagram
-
involved in salvage of adenine and methionine from 5'-methylthioadenosine
-
-
?
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
hydrogen peroxide
reversible inactivation, 60% decrease of enzymatic activity at 0.5 mM hydrogen peroxide
tert-butyl hydroperoxide
0.01 mM
2-bromo-9-(1,3-dihydroxy-2-propoxymethyl)adenine
-
strong
2-bromo-9-(2-hydroxyethoxymethyl)adenine
-
-
2-chloro-9-(1,3-dihydroxy-2-propoxymethyl)adenine
-
strong
2-chloro-9-(2-hydroxyethoxymethyl)adenine
-
-
2-iodo-9-(1,3-dihydroxy-2-propoxymethyl)adenine
-
strong
2-iodo-9-(2-hydroxyethoxymethyl)adenine
-
-
5'-Deoxy-5'-chloroformycin
-
competitive
9-(phosphonoalkyl)adenine
-
-
9-(phosphonoheptyl)adenine
-
-
9-[(1-Hydroxy-3-iodo-2-propoxy)methyl]adenine
-
competitive
additional information
MTAP is inactivated in the liver of lipopolysaccharide-challenged mice
-
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.004
5'-deoxy-5'-methylthioadenosine
-
-
3.5
phosphate
-
-
Ki VALUE [mM]
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.0002 - 0.0007
2-bromo-9-(1,3-dihydroxy-2-propoxymethyl)adenine
-
-
0.001 - 0.01
2-bromo-9-(2-hydroxyethoxymethyl)adenine
-
-
0.0002 - 0.0007
2-chloro-9-(1,3-dihydroxy-2-propoxymethyl)adenine
-
-
0.001 - 0.01
2-chloro-9-(2-hydroxyethoxymethyl)adenine
-
-
0.0002 - 0.0007
2-iodo-9-(1,3-dihydroxy-2-propoxymethyl)adenine
-
-
0.001 - 0.01
2-iodo-9-(2-hydroxyethoxymethyl)adenine
-
-
0.015
9-(phosphonoheptyl)adenine
-
low phosphate concentration of 3.5 mM
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
-
UniProt
Manually annotated by BRENDA team
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
additional information
-
RRK081 cells, heterozygous for the MtaplacZ allele
Manually annotated by BRENDA team
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
malfunction
-
mice homozygous for a MTAP null allele (MtaplacZ) have an embryonic lethal phenotype dying around day 8 postconception. Mtap/MtaplacZ heterozygotes are born at Mendelian frequencies and appear indistinguishable from wild-type mice during the first year of life, but tend to die prematurely with a median survival of 585 days. These animals have greatly enlarged spleens, altered thymic histology, and lymphocytic infiltration of their livers, consistent with lymphoma
physiological function
-
Mtap is essential during embryogenesis. Mtap is a tumor suppressor gene independent of CDKN2A and ARF
UNIPROT
ENTRY NAME
ORGANISM
NO. OF AA
NO. OF TRANSM. HELICES
MOLECULAR WEIGHT[Da]
SOURCE
SEQUENCE
LOCALIZATION PREDICTION?
MTAP_MOUSE
283
0
31062
Swiss-Prot
other Location (Reliability: 2)
PROTEIN VARIANTS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
C136S
mutant is insensitive to oxidative inhibition
C223S
mutant is insensitive to oxidative inhibition
PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
Ni2+-Sepharose column chromatography
CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
expressed in Escherichia coli BL21(DE3) cells
RRK081 embryonic stem cells, containing a gene-trap vector inserted between exon 3 and exon 4 of the mouse Mtap locus (MtaplacZ) injected into mouse blastocysts derived from C57BL/6 animals, embryos implanted in pseudopregnant females
-
EXPRESSION
ORGANISM
UNIPROT
LITERATURE
lymphoma-infiltrated tissues tend to have reduced levels of Mtap mRNA and MTAP protein in addition to unaltered levels of methyldeoxycytidine. Loss of MTAP expression is independent from loss of CDKN2A expression
-
REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Savarese, T.M.; Harrington, S.; Nakamura, C.; Chen, Z.H.; Kumar, P.; Mikkilineni, A.; Abushanab, E.; Chu, S.H.; Parks, R.E.
5'-Deoxy-5'-methylthioadenosine phosphorylase. V. Acycloadenosine derivatives as inhibitors of the enzyme
Biochem. Pharmacol.
40
2465-2471
1990
Homo sapiens, Mus musculus
Manually annotated by BRENDA team
Savarese, T.M.; Crabtree, G.W.; Parks, R.E.
5'-Methylthioadenosine phosphorylase. I. Substrate activity of 5'-deoxyadenosine with the enzyme from Sarcoma 180 cells
Biochem. Pharmacol.
30
189-199
1981
Mus musculus
Manually annotated by BRENDA team
Fernandez-Irigoyen, J.; Santamaria, M.; Sanchez-Quiles, V.; Latasa, M.U.; Santamaria, E.; Munoz, J.; Sanchez Del Pino, M.M.; Valero, M.L.; Prieto, J.; Avila, M.A.; Corrales, F.J.
Redox regulation of methylthioadenosine phosphorylase in liver cells: molecular mechanism and functional implications
Biochem. J.
411
457-465
2008
Homo sapiens, Mus musculus (Q9CQ65)
Manually annotated by BRENDA team
Kadariya, Y.; Yin, B.; Tang, B.; Shinton, S.A.; Quinlivan, E.P.; Hua, X.; Klein-Szanto, A.; Al-Saleem, T.I.; Bassing, C.H.; Hardy, R.R.; Kruger, W.D.
Mice heterozygous for germ-line mutations in methylthioadenosine phosphorylase (MTAP) die prematurely of T-cell lymphoma
Cancer Res.
69
5961-5969
2009
Mus musculus
Manually annotated by BRENDA team