Information on EC 2.4.2.27 - dTDP-dihydrostreptose-streptidine-6-phosphate dihydrostreptosyltransferase

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The expected taxonomic range for this enzyme is: Streptomyces griseus

EC NUMBER
COMMENTARY
2.4.2.27
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RECOMMENDED NAME
GeneOntology No.
dTDP-dihydrostreptose-streptidine-6-phosphate dihydrostreptosyltransferase
REACTION
REACTION DIAGRAM
COMMENTARY
ORGANISM
UNIPROT ACCESSION NO.
LITERATURE
dTDP-L-dihydrostreptose + streptidine 6-phosphate = dTDP + O-1,4-alpha-L-dihydrostreptosyl-streptidine 6-phosphate
show the reaction diagram
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REACTION TYPE
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
pentosyl group transfer
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-
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PATHWAY
KEGG Link
MetaCyc Link
Biosynthesis of secondary metabolites
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Streptomycin biosynthesis
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streptomycin biosynthesis
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SYSTEMATIC NAME
IUBMB Comments
dTDP-L-dihydrostreptose:streptidine-6-phosphate dihydrostreptosyltransferase
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SYNONYMS
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
dihydrostreptosyltransferase, thymidine diphosphodihydrostreptose-streptidine 6-phosphate
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CAS REGISTRY NUMBER
COMMENTARY
73699-20-4
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ORGANISM
COMMENTARY
LITERATURE
SEQUENCE CODE
SEQUENCE DB
SOURCE
SUBSTRATE
PRODUCT                      
REACTION DIAGRAM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
(Substrate)
LITERATURE
(Substrate)
COMMENTARY
(Product)
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
dTDP-L-dihydrostreptose + streptidine 6-phosphate
dTDP + O-alpha-L-dihydrostreptosyl-1,4-streptidine 6-phosphate
show the reaction diagram
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-
?
dTDP-L-dihydrostreptose + streptidine 6-phosphate
?
show the reaction diagram
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biosynthesis of streptomycin
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additional information
?
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not: streptidine, 2-deoxystreptamine, 4-deoxystreptamine
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NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
(Substrate)
LITERATURE
(Substrate)
COMMENTARY
(Product)
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
dTDP-L-dihydrostreptose + streptidine 6-phosphate
?
show the reaction diagram
-
biosynthesis of streptomycin
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METALS and IONS
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
Co2+
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as effective as Mg2+
Mg2+
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Mn2+ or Mg2+ required, maximal activity with 10 mM Mg2+
Mn2+
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Mn2+ or Mg2+ required, maximal activity with 3 mM Mn2+
INHIBITORS
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
IMAGE
SPECIFIC ACTIVITY [µmol/min/mg]
SPECIFIC ACTIVITY MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
additional information
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pH OPTIMUM
pH MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
8.2
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glycine/NaOH buffer
additional information
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no distinct pH-optimum observed in Tris-HCl buffer between pH 7.3 and 9
TEMPERATURE OPTIMUM
TEMPERATURE OPTIMUM MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
0
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assay at
MOLECULAR WEIGHT
MOLECULAR WEIGHT MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
63000
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gel filtration
SUBUNITS
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
dimer
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2 * 35000, SDS-PAGE in presence of 2-mercaptoethanol
GENERAL STABILITY
ORGANISM
UNIPROT ACCESSION NO.
LITERATURE
addition of streptidine is important for stabilization of enzyme activity during purification, purified transferase is also stable in absence of streptidine
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STORAGE STABILITY
ORGANISM
UNIPROT ACCESSION NO.
LITERATURE
4°C, 50 mM Tris-HCl, pH 7.8 at 0°C, containing 5 mM mercaptoethanol, 1 mM EDTA, 2.5 mM streptidine, 10% v/v glycerol, stable for at least 4 months
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Purification/COMMENTARY
ORGANISM
UNIPROT ACCESSION NO.
LITERATURE
ammonium sulfate precipitation, Sephadex G-200, chromatography on DEAE-cellulose, Ultrogel AcA44 column, DEAE-Sepharose column
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