Information on EC 2.4.2.25 - flavone apiosyltransferase

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The expected taxonomic range for this enzyme is: Petroselinum crispum

EC NUMBER
COMMENTARY
2.4.2.25
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RECOMMENDED NAME
GeneOntology No.
flavone apiosyltransferase
REACTION
REACTION DIAGRAM
COMMENTARY
ORGANISM
UNIPROT
LITERATURE
UDP-alpha-D-apiose + apigenin 7-O-beta-D-glucoside = UDP + apigenin 7-O-[beta-D-apiosyl-(1->2)-beta-D-glucoside]
show the reaction diagram
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REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
pentosyl group transfer
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-
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PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
apigenin glycosides biosynthesis
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Flavone and flavonol biosynthesis
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SYSTEMATIC NAME
IUBMB Comments
UDP-apiose:5,4'-dihydroxyflavone 7-O-beta-D-glucoside 2''-O-beta-D-apiofuranosyltransferase
7-O-beta-D-Glucosides of a number of flavonoids and of 4-substituted phenols can act as acceptors.
SYNONYMS
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
apiosyltransferase, uridine diphosphoapiose-flavone
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UDPapiose:7-O-(beta-D-glucosyl)-flavone apiosyltransferase
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CAS REGISTRY NUMBER
COMMENTARY
37332-49-3
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ORGANISM
COMMENTARY
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
SUBSTRATE
PRODUCT                      
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
LITERATURE
(Substrate)
COMMENTARY
(Product)
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
UDPapiose + 7-O-(beta-D-glucosyl)-apigenin
UDP + 7-O-beta-D-apiofuranosyl-1,2-beta-D-glucosyl-apigenin
show the reaction diagram
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specific for UDPapiose as glycosyl donor
i.e. apiin
?
UDPapiose + 7-O-(beta-D-glucosyl)-apigenin
?
show the reaction diagram
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biosynthesis of apiin
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UDPapiose + biochanin A
?
show the reaction diagram
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i.e. 5,7-dihydroxy-4'-methoxyisoflavone-7-glucoside
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?
additional information
?
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overview: 7-O-beta-glucosides of a number of flavonoids and of 4-substituted phenols can act as acceptors, not: flavonol-3-glucoside, flavonol-7-glucoside, apigenin-8-C-glucoside, aglycones of flavonoids, glucose
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NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
LITERATURE
(Substrate)
COMMENTARY
(Product)
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
UDPapiose + 7-O-(beta-D-glucosyl)-apigenin
?
show the reaction diagram
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biosynthesis of apiin
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INHIBITORS
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
apigenin-7-glucoside
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Chrysoeriol-7-glucoside
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iodoacetamide
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at 1 mM causes an inhibition of 55%, reversed by cysteine
p-chloromercuribenzoate
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at 1 mM inhibits the reaction completely
additional information
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no effect: EDTA, NH4+, K+
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ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
dithioerythritol
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stimulates enzyme activity up to 35%, optimum concentration: 6 mM
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
0.066
apigenin-7-glucoside
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SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
additional information
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pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
7
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higher apiin yield in Tris-HCl than in phosphate buffer
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
SOURCE
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY
GeneOntology No.
LITERATURE
SOURCE
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
50000
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gel filtration
637786
pH STABILITY
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
6 - 8
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highest stability
637786
GENERAL STABILITY
ORGANISM
UNIPROT
LITERATURE
albumin, 0.1 mg, significant stabilization
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solution of enzyme after hydroxylapatite chromatography in sodium phosphate buffer, pH 7.0, rapidly loses activity upon freezing
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STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
-20°C, Tris-HCl, dithiothreitol, protein concentration 0.5-1 mg/ml
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Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
purified 123fold by protamine sulfate and ammonium sulfate precipitation and chromatography on DEAE-cellulose, SephadexG-100 and hydroxylapatite
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