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Information on EC 2.4.2.22 - xanthine phosphoribosyltransferase and Organism(s) Escherichia coli and UniProt Accession P0A9M5

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EC Tree
     2 Transferases
         2.4 Glycosyltransferases
             2.4.2 Pentosyltransferases
                2.4.2.22 xanthine phosphoribosyltransferase
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This record set is specific for:
Escherichia coli
UNIPROT: P0A9M5 not found.
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Word Map
The taxonomic range for the selected organisms is: Escherichia coli
The enzyme appears in selected viruses and cellular organisms
Synonyms
xprt, xgprt, xanthine phosphoribosyltransferase, hgxprt, hypoxanthine-guanine-xanthine phosphoribosyltransferase, xprtase, pf hgxprt, hypoxanthine-guanine-(xanthine) phosphoribosyltransferase, ecxgprt, xanthine prt, more
SYNONYM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
xanthine-guanine PRT
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5-phospho-alpha-D-ribose-1-diphosphate:xanthine phospho-D-ribosyltransferase
-
-
-
-
phosphoribosyltransferase, xanthine
-
-
-
-
Xan phosphoribosyltransferase
-
-
-
-
xanthosine 5'-phosphate pyrophosphorylase
-
-
-
-
xanthylate pyrophosphorylase
-
-
-
-
xanthylic pyrophosphorylase
-
-
-
-
XGPRT
-
-
-
-
XMP pyrophosphorylase
-
-
-
-
XPRT
-
-
-
-
XPRTase
-
-
-
-
additional information
see also EC 2.4.2.8
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
pentosyl group transfer
-
-
-
-
SYSTEMATIC NAME
IUBMB Comments
XMP:diphosphate 5-phospho-alpha-D-ribosyltransferase
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CAS REGISTRY NUMBER
COMMENTARY hide
9023-10-3
-
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
5-phospho-alpha-D-ribose 1-diphosphate + guanine
GMP + diphosphate
show the reaction diagram
-
-
-
?
5-phospho-alpha-D-ribose 1-diphosphate + hypoxanthine
IMP + diphosphate
show the reaction diagram
-
-
-
?
5-phospho-alpha-D-ribose 1-diphosphate + xanthine
9-(5-phospho-beta-D-ribosyl)xanthine + diphosphate
show the reaction diagram
-
-
-
?
guanine + 5-phospho-alpha-D-ribose 1-diphosphate
GMP + diphosphate
show the reaction diagram
-
-
-
r
hypoxanthine + 5-phospho-alpha-D-ribose 1-diphosphate
IMP + diphosphate
show the reaction diagram
low activity, reaction of EC 2.4.2.8
-
-
r
xanthine + 5-phospho-alpha-D-ribose 1-diphosphate
XMP + diphosphate
show the reaction diagram
-
-
-
r
5-phospho-alpha-D-ribose 1-diphosphate + xanthine
9-(5-phospho-beta-D-ribosyl)xanthine + diphosphate
show the reaction diagram
-
-
-
?
additional information
?
-
Escherichia coli xanthine-guanine phosphoribosyltransferase (EcXGPRT) prefers guanine and xanthine
-
-
-
NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
guanine + 5-phospho-alpha-D-ribose 1-diphosphate
GMP + diphosphate
show the reaction diagram
-
-
-
r
xanthine + 5-phospho-alpha-D-ribose 1-diphosphate
XMP + diphosphate
show the reaction diagram
-
-
-
r
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
(2-[(2,3-dihydroxypropyl)[2-(6-oxo-1,6-dihydro-9H-purin-9-yl)ethyl]amino]ethyl)phosphonic acid
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(2-[[2-(2-amino-6-oxo-1,6-dihydro-9H-purin-9-yl)ethyl][2-(2-phosphonoethoxy)ethyl]amino]ethyl)phosphonic acid
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9-[(N-phosphonoethyl-N-phosphonobutyl)-2-aminoethyl]hypoxanthine
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9-[(N-phosphonoethyl-N-phosphonoethoxyethyl)-2-aminoethyl]-hypoxanthine
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9-[(N-phosphonoethyl-N-phosphonomethoxyethyl)-2-aminoethyl]hypoxanthine
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9-[(N-phosphonoethyl-N-phosphonomethyl)-2-aminoethyl]-hypoxanthine
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[[2-[(6-oxo-1,6-dihydro-9H-purin-9-yl)methyl]propane-1,3-diyl]bis(oxymethylene)]bis(phosphonic acid)
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[[[(4-oxo-4,5-dihydro-3H-pyrrolo[3,2-d]pyrimidin-7-yl)methyl]azanediyl]di(propane-3,1-diyl)]bis(phosphonic acid)
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additional information
EcXGPRT inhibitor design, synthesis and optimization. Several acyclic nucleoside phosphonates (ANPs) have previously been identified as inhibitors of EcXGPRT and EcHPRT and the most potent of these have Ki values as low as 10 nM for EcXGPRT and 0.8 mM for EcHPRT
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KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.139
5-phospho-alpha-D-ribose 1-diphosphate
-
0.0043
guanine
-
0.0908 - 0.091
hypoxanthine
0.0043 - 0.0305
xanthine
TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
112
5-phospho-alpha-D-ribose 1-diphosphate
-
54.8
hypoxanthine
-
150
xanthine
-
kcat/KM VALUE [1/mMs-1]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.0065
guanine
pH and temperature not specified in the publication
0.0012
xanthine
pH and temperature not specified in the publication
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
metabolism
in Escherichia coli, the purine salvage pathway has two 6-oxopurine phosphoribosyltransferases (PRTs), xanthine-guanine PRT (EcXGPRT, EC 2.4.2.22) and hypoxanthine PRT (EcHPRT, EC 2.4.2.8). Escherichia coli can utilize both purine salvage and de novo pathways for the production of the nucleoside monophosphates required for incorporation into DNA and RNA. Escherichia coli is highly unusual in that it is one of only a few organisms that possess two distinct salvage enzymes for 6-oxopurine nucleoside monophosphate production
CRYSTALLIZATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
hanging drop vapor diffusion method
REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Krenitsky, T.A.; Neil, S.M.; Miller, R.L.
Guanine and xanthine phosphoribosyltransfer activities of Lactobacillus casei and Escherichia coli. Their relationship to hypoxanthine and adenine phosphoribosyltransfer activities
J. Biol. Chem.
245
2605-2611
1970
Escherichia coli, Lacticaseibacillus casei
Manually annotated by BRENDA team
Vos, S.; de Jersey, J.; Martin, J.L.
Crystal structure of Escherichia coli xanthine phosphoribosyltransferase
Biochemistry
36
4125-4134
1997
Escherichia coli (P0A9M5), Escherichia coli
Manually annotated by BRENDA team
Eng, W.; Hockova, D.; Spacek, P.; Baszczynski, O.; Janeba, Z.; Naesens, L.; Keough, D.; Guddat, L.
Crystal structures of acyclic nucleoside phosphonates in complex with Escherichia coli hypoxanthine phosphoribosyltransferase
ChemistrySelect
1
6267-6276
2016
Escherichia coli (P0A9M5)
-
Manually annotated by BRENDA team