Information on EC 2.4.2.20 - dioxotetrahydropyrimidine phosphoribosyltransferase

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The expected taxonomic range for this enzyme is: Bos taurus

EC NUMBER
COMMENTARY hide
2.4.2.20
-
RECOMMENDED NAME
GeneOntology No.
dioxotetrahydropyrimidine phosphoribosyltransferase
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
a 2,4-dioxotetrahydropyrimidine D-ribonucleotide + diphosphate = a 2,4-dioxotetrahydropyrimidine + 5-phospho-alpha-D-ribose 1-diphosphate
show the reaction diagram
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-
-
-
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
pentosyl group transfer
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-
-
-
SYSTEMATIC NAME
IUBMB Comments
2,4-dioxotetrahydropyrimidine-D-ribonucleotide:diphosphate phospho-alpha-D-ribosyltransferase
Acts (in the reverse direction) on uracil and other pyrimidines and pteridines containing a 2,4-diketo structure.
CAS REGISTRY NUMBER
COMMENTARY hide
37277-75-1
-
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
-
-
-
Manually annotated by BRENDA team
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
uric acid + 5-phospho-alpha-D-ribose 1-diphosphate
3-ribosyluric acid 5'-phosphate + diphosphate
show the reaction diagram
-
-
-
?
xanthine + 5-phospho-alpha-D-ribose 1-diphosphate
3-ribosylxanthine 5'-phosphate + diphosphate
show the reaction diagram
-
-
-
?
additional information
?
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enzyme also synthesizes a number of pyrimidine ribonucleotides from the corresponding base, e.g. uracil, orotic acid, thymine, 6-azathymine, 6-azauracil, 5-fluorouracil or 5-iodouracil and diphosphorylribose phosphate
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-
-
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Mg2+
-
required at 2 mM or higher for maximal activity
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
3-ribosylxanthine 5'-phosphate
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xanthine and uracil pyrophosphorylase
UMP
-
xanthine and uracil pyrophosphorylase
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
0.497
-
-
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
6
-
for uric acid reaction
7.6 - 8.6
-
for xanthine reaction
8 - 9.2
-
for orotic acid reaction
9.5
-
for uracil reaction
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
37
-
assay at
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
TEMPERATURE STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
50
-
pH 6.0, 40 min, 12-22% loss of activity
Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
purified 5400fold, ammonium sulfate fractionation, chromatography on DEAE-cellulose, treatment with Alumina CY Gel, calcium phosphate gel adsorption and elution and purification with hydroxylapatite
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