Information on EC 2.4.2.20 - dioxotetrahydropyrimidine phosphoribosyltransferase

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The expected taxonomic range for this enzyme is: Bos taurus

EC NUMBER
COMMENTARY
2.4.2.20
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RECOMMENDED NAME
GeneOntology No.
dioxotetrahydropyrimidine phosphoribosyltransferase
REACTION
REACTION DIAGRAM
COMMENTARY
ORGANISM
UNIPROT ACCESSION NO.
LITERATURE
a 2,4-dioxotetrahydropyrimidine D-ribonucleotide + diphosphate = a 2,4-dioxotetrahydropyrimidine + 5-phospho-alpha-D-ribose 1-diphosphate
show the reaction diagram
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REACTION TYPE
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
pentosyl group transfer
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SYSTEMATIC NAME
IUBMB Comments
2,4-dioxotetrahydropyrimidine-D-ribonucleotide:diphosphate phospho-alpha-D-ribosyltransferase
Acts (in the reverse direction) on uracil and other pyrimidines and pteridines containing a 2,4-diketo structure.
SYNONYMS
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
2,4-dioxotetrahydropyrimidine-nucleotide:pyrophosphate phospho-alpha-D-ribosyltransferase
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dioxotetrahydropyrimidine phosphoribosyl transferase
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dioxotetrahydropyrimidine ribonucleotide pyrophosphorylase
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dioxotetrahydropyrimidine-ribonucleotide pyrophosphorylase
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phosphoribosyltransferase, dioxotetrahydropyrimidine
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uracil pyrophosphorylase
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CAS REGISTRY NUMBER
COMMENTARY
37277-75-1
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ORGANISM
COMMENTARY
LITERATURE
SEQUENCE CODE
SEQUENCE DB
SOURCE
SUBSTRATE
PRODUCT                      
REACTION DIAGRAM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
(Substrate)
LITERATURE
(Substrate)
COMMENTARY
(Product)
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
uric acid + 5-phospho-alpha-D-ribose 1-diphosphate
3-ribosyluric acid 5'-phosphate + diphosphate
show the reaction diagram
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?
xanthine + 5-phospho-alpha-D-ribose 1-diphosphate
3-ribosylxanthine 5'-phosphate + diphosphate
show the reaction diagram
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?
additional information
?
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enzyme also synthesizes a number of pyrimidine ribonucleotides from the corresponding base, e.g. uracil, orotic acid, thymine, 6-azathymine, 6-azauracil, 5-fluorouracil or 5-iodouracil and diphosphorylribose phosphate
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METALS and IONS
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
Mg2+
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required at 2 mM or higher for maximal activity
INHIBITORS
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
IMAGE
3-ribosylxanthine 5'-phosphate
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xanthine and uracil pyrophosphorylase
UMP
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xanthine and uracil pyrophosphorylase
SPECIFIC ACTIVITY [µmol/min/mg]
SPECIFIC ACTIVITY MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
0.497
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pH OPTIMUM
pH MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
6
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for uric acid reaction
7.6
8.6
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for xanthine reaction
8
9.2
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for orotic acid reaction
9.5
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for uracil reaction
TEMPERATURE OPTIMUM
TEMPERATURE OPTIMUM MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
37
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assay at
SOURCE TISSUE
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
SOURCE
TEMPERATURE STABILITY
TEMPERATURE STABILITY MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
50
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pH 6.0, 40 min, 12-22% loss of activity
Purification/COMMENTARY
ORGANISM
UNIPROT ACCESSION NO.
LITERATURE
purified 5400fold, ammonium sulfate fractionation, chromatography on DEAE-cellulose, treatment with Alumina CY Gel, calcium phosphate gel adsorption and elution and purification with hydroxylapatite
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