Information on EC 2.4.2.17 - ATP phosphoribosyltransferase

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The expected taxonomic range for this enzyme is: Eukaryota, Bacteria

EC NUMBER
COMMENTARY
2.4.2.17
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RECOMMENDED NAME
GeneOntology No.
ATP phosphoribosyltransferase
REACTION TYPE
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
pentosyl group transfer
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-
-
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PATHWAY
KEGG Link
MetaCyc Link
Biosynthesis of secondary metabolites
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histidine biosynthesis
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Histidine metabolism
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Metabolic pathways
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SYSTEMATIC NAME
IUBMB Comments
1-(5-phospho-D-ribosyl)-ATP:diphosphate phospho-alpha-D-ribosyl-transferase
Involved in histidine biosynthesis.
SYNONYMS
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
1-(5-phospho-D-ribosyl)-ATP:pyrophosphate phospho-alpha-D-ribosyltransferase
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-
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adenosine triphosphate phosphoribosyltransferase
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-
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ATP phosphoribosyl transferase
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ATP phosphoribosyl transferase
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ATP phosphoribosyl transferase
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ATP phosphoribosyl transferase complex
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ATP phosphoribosyltransferase
Q9Z472
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ATP phosphoribosyltransferase
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ATP-phosphoribosyltransferase
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ATP-PRT
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-
-
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ATP-PRT
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ATP-PRT1
Q56UT3
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ATP-PRT1
Q56US5
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ATP-PRT2
Q56UT2
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ATP-PRTase
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N-1-(5'-phosphoribosyl)-ATP transferase
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phosphoribosyl ATP synthetase
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-
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phosphoribosyl ATP:pyrophosphate phosphoribosyltransferase
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phosphoribosyl-ATP pyrophosphorylase
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phosphoribosyl-ATP:pyrophosphate-phosphoribosyl phosphotransferase
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phosphoribosyladenosine triphosphate pyrophosphorylase
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-
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phosphoribosyladenosine triphosphate synthetase
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phosphoribosyltransferase, adenosine triphosphate
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-
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CAS REGISTRY NUMBER
COMMENTARY
9031-46-3
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GENERAL INFORMATION
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
metabolism
-, Q9Z472
essential for histidine biosynthesis
SPECIFIC ACTIVITY [µmol/min/mg]
SPECIFIC ACTIVITY MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
0.39
-
-, Q9Z472
N215K/L231F/T235A/A270P mutant protein, pH 8.5, temperature not specified in the publication
1.96
-
-, Q9Z472
A270P mutant protein, pH 8.5, temperature not specified in the publication
2.01
-
-, Q9Z472
N215K mutant protein, pH 8.5, temperature not specified in the publication
2.04
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-, Q9Z472
L231F/T235A mutant protein, pH 8.5, temperature not specified in the publication
2.1
-
-, Q9Z472
N215K/L231F/T235A mutant protein, pH 8.5, temperature not specified in the publication
2.19
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-, Q9Z472
wild type protein, pH 8.5, temperature not specified in the publication
additional information
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-
description of assay method
additional information
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data for genetically-modified Corynebacterium glutamicum
pH RANGE
pH RANGE MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
7.5
13
-, Q9Z472
>40% relative activity, rapid loss of activity below pH 7.5
TEMPERATURE RANGE
TEMPERATURE MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
SOURCE TISSUE
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
SOURCE
-, Q56UT2, Q56UT3
transcript levels is constitutively higher in Alyssum lesbiacum (a plant that hyperaccumulates Ni and exhibits an exceptional degree of Ni tolerance) than in the congeneric nonaccumulator Alyssum montanum, transcript levels in the weak hyperaccumulator Alyssum serpyllifolium is intermediate; transcript levels is constitutively higher in Alyssum lesbiacum (a plant that hyperaccumulates Ni and exhibits an exceptional degree of Ni tolerance) than in the congeneric nonaccumulator Alyssum montanum, transcript levels in the weak hyperaccumulator Alyssum serpyllifolium is intermediate
Manually annotated by BRENDA team
Q56US5
transcript levels is constitutively higher in Alyssum lesbiacum (a plant that hyperaccumulates Ni and exhibits an exceptional degree of Ni tolerance) than in the congeneric nonaccumulator Alyssum montanum, transcript levels in the weak hyperaccumulator Alyssum serpyllifolium is intermediate
Manually annotated by BRENDA team
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transcript levels is constitutively higher in Alyssum lesbiacum (a plant that hyperaccumulates Ni and exhibits an exceptional degree of Ni tolerance) than in the congeneric nonaccumulator Alyssum montanum, transcript levels in the weak hyperaccumulator Alyssum serpyllifolium is intermediate
Manually annotated by BRENDA team
-, Q56UT2, Q56UT3
transcript levels is constitutively higher in Alyssum lesbiacum (a plant that hyperaccumulates Ni and exhibits an exceptional degree of Ni tolerance) than in the congeneric nonaccumulator Alyssum montanum, transcript levels in the weak hyperaccumulator Alyssum serpyllifolium is intermediate; transcript levels is constitutively higher in Alyssum lesbiacum (a plant that hyperaccumulates Ni and exhibits an exceptional degree of Ni tolerance) than in the congeneric nonaccumulator Alyssum montanum, transcript levels in the weak hyperaccumulator Alyssum serpyllifolium is intermediate
Manually annotated by BRENDA team
Q56US5
transcript levels is constitutively higher in Alyssum lesbiacum (a plant that hyperaccumulates Ni and exhibits an exceptional degree of Ni tolerance) than in the congeneric nonaccumulator Alyssum montanum, transcript levels in the weak hyperaccumulator Alyssum serpyllifolium is intermediate
Manually annotated by BRENDA team
-
transcript levels is constitutively higher in Alyssum lesbiacum (a plant that hyperaccumulates Ni and exhibits an exceptional degree of Ni tolerance) than in the congeneric nonaccumulator Alyssum montanum, transcript levels in the weak hyperaccumulator Alyssum serpyllifolium is intermediate
Manually annotated by BRENDA team
PDB
SCOP
CATH
ORGANISM
Bacillus subtilis (strain 168)
Escherichia coli (strain K12)
Escherichia coli (strain K12)
Lactococcus lactis subsp. lactis (strain IL1403)
Lactococcus lactis subsp. lactis (strain IL1403)
Methanothermobacter thermautotrophicus (strain ATCC 29096 / DSM 1053 / JCM 10044 / NBRC 100330 / Delta H)
Thermotoga maritima (strain ATCC 43589 / MSB8 / DSM 3109 / JCM 10099)
Thermotoga maritima (strain ATCC 43589 / MSB8 / DSM 3109 / JCM 10099)
Thermotoga maritima (strain ATCC 43589 / MSB8 / DSM 3109 / JCM 10099)
Thermus thermophilus (strain HB27 / ATCC BAA-163 / DSM 7039)
pH STABILITY
pH STABILITY MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
additional information
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overview: stability at various pH-values
TEMPERATURE STABILITY
TEMPERATURE STABILITY MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
45
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very sensitive to heat inactivation, 0.4 mM histidine stabilizes the enzyme to inactivation by heat
47.5
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1.9 mg enzyme/ml, 80 min, 75% loss of activity without addition of AMP, with 0.05 mM AMP about 60% loss of activity, with 0.5 mM AMP about 40% loss of activity, with 5 mM AMP about 25% loss of activity
48
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3 mg enzyme/ml, 10 min, 15% remaining activity without addition of histidine, with 0.000086 mM histidine about 15% remaining activity after 15 min, with 0.00069 mM histidine about 70% remaining activity after 80 min, with 0.00345 mM histidine about 80% remaining activity after 80 min, with 0.0069 mM histidine about 55% remaining activity after 50 min
additional information
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heat inactivation depends on protein concentration and inhibitors
GENERAL STABILITY
ORGANISM
UNIPROT ACCESSION NO.
LITERATURE
histidine or AMP stabilizes the enzyme with respect to thermal inactivation
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L-histidine, 0.4 mM, stabilizes against heat inactivation, 0.04 mM does not stabilize against heat inactivation, at 1.33 mM and higher heat inactivation is greater than the control
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NaCl and 2-mercaptoethanol stabilize the very labile enzyme
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overview, stability of the enzyme at various pH-values, salt concentrations and histidine concentrations
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slight stabilization by 10 mM MgCl2 or CaCl2 by 1 mM MnCl2 and by 1 mM histidine at pH-values above 7
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STORAGE STABILITY
ORGANISM
UNIPROT ACCESSION NO.
LITERATURE
-20C, 50 mM Tris-HCl, pH 7.5, 0.4 mM DTT and one protease inhibitor per litre, 50% v/v glycerol, long term storage
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4C, 0.01 M Tris, 0.10 M NaCl, 0.4 mM histidine, 2.8 mM 2-mercaptoethanol, 0.5 mM EDTA, pH 7.5, 50% loss of activity after several days
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4C, HEPES buffer pH 7.5, final ammonium sulfate microcrystalline enzyme in 60% saturated ammonium sulfate, 0.1 M NaCl, 0.01 M Tris, 1.5 mM EDTA, 10 mM dithiothreitol, stable for 1 month, greater than 95% activity retained
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storage in liquid nitrogen of quick-frozen enzyme in HEPES buffer pH 7.5, 0.1 M NaCl, 0.01 M Tris, 0.5 mM EDTA, 1 mM dithiothreitol, 1 mM histidine, indefinitely stable, preserves 100% activity. The critical factor for stability seems to be the maintenance of a sulfhydryl-reducing environment, high dithiothreitol concentrations has no adverse effect at either 0C or 37C
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