Information on EC 2.4.2.16 - urate-ribonucleotide phosphorylase

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The expected taxonomic range for this enzyme is: Eutheria

EC NUMBER
COMMENTARY
2.4.2.16
-
RECOMMENDED NAME
GeneOntology No.
urate-ribonucleotide phosphorylase
REACTION
REACTION DIAGRAM
COMMENTARY
ORGANISM
UNIPROT ACCESSION NO.
LITERATURE
urate D-ribonucleotide + phosphate = urate + alpha-D-ribose 1-phosphate
show the reaction diagram
-
-
-
-
REACTION TYPE
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
pentosyl group transfer
-
-
-
-
PATHWAY
KEGG Link
MetaCyc Link
Purine metabolism
-
SYSTEMATIC NAME
IUBMB Comments
urate-D-ribonucleotide:phosphate alpha-D-ribosyltransferase
-
SYNONYMS
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
phosphorylase, urate ribonucleotide
-
-
-
-
UAR phosphorylase
-
-
-
-
urate ribonucleotide phosphorylase
-
-
-
-
urate-ribonucleotide:orthophosphate D-ribosyltransferase
-
-
-
-
CAS REGISTRY NUMBER
COMMENTARY
9030-29-9
-
ORGANISM
COMMENTARY
LITERATURE
SEQUENCE CODE
SEQUENCE DB
SOURCE
Pigeon
-
-
-
Manually annotated by BRENDA team
SUBSTRATE
PRODUCT                      
REACTION DIAGRAM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
(Substrate)
LITERATURE
(Substrate)
COMMENTARY
(Product)
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
uric acid ribonucleoside + arsenate
urate + D-ribose 1-phosphate
show the reaction diagram
-
-
-
?
uric acid ribonucleoside + arsenate
urate + D-ribose 1-phosphate
show the reaction diagram
-
reverse reaction not demonstrated, arsenate can replace phosphate at a lower reaction rate
-
ir
uric acid ribonucleoside + phosphate
urate + D-ribose 1-phosphate
show the reaction diagram
-
-
-
?
uric acid ribonucleoside + phosphate
urate + D-ribose 1-phosphate
show the reaction diagram
-
-
-
ir
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
(Substrate)
LITERATURE
(Substrate)
COMMENTARY
(Product)
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
uric acid ribonucleoside + phosphate
urate + D-ribose 1-phosphate
show the reaction diagram
-
-
-
?
uric acid ribonucleoside + phosphate
urate + D-ribose 1-phosphate
show the reaction diagram
-
-
-
ir
INHIBITORS
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
IMAGE
1-Ribosyluracil
-
1 mM, about 85% inhibition
2-thio-6-oxopurine
-
1 mM, 73% inhibition
2-Thiouracil
-
1 mM, 96% inhibition
4-amino-5-imidazolecarboxamide
-
1 mM, about 15% inhibition
5-methyluracil
-
1 mM, about 90% inhibition
5-methyluridine
-
1 mM, about 95% inhibition
Colchicine
-
1 mM, 43% inhibition, competitive
guanosine
-
1 mM, about 20% inhibition
Inosine
-
1 mM, about 25% inhibition
p-chloromercuribenzoate
-
reversed by cysteine
Phenylbutazone
-
1 mM, 30% inhibition, competitive
Uracil
-
1 mM, about 85% inhibition
xanthine
-
1 mM, 35% inhibition
Ki VALUE [mM]
Ki VALUE [mM] Maximum
INHIBITOR
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
IMAGE
1.23
-
Colchicine
-
37C, pH 8.2
1.76
-
Phenylbutazone
-
37C, pH 8.2
SPECIFIC ACTIVITY [µmol/min/mg]
SPECIFIC ACTIVITY MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
pH OPTIMUM
pH MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
7.5
8
-
reaction with phosphate or arsenate
pH RANGE
pH RANGE MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
6
9.5
-
pH 6.0: about 60% of maximal activity, pH 9.5: 45% of maximal activity
pH STABILITY
pH STABILITY MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
4.5
-
-
0C, 10 min, 30% loss of activity, partially purified enzyme
TEMPERATURE STABILITY
TEMPERATURE STABILITY MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
50
-
-
pH 7.8, 5 min, 5% loss of activity, partially purified enzyme
60
-
-
pH 7.8, 5 min, 40% loss of activity, partially purified enzyme
70
-
-
pH 7.8, 5 min, 95% loss of activity, partially purified enzyme
80
-
-
pH 7.8, 5 min, 100% loss of activity, partially purified enzyme
STORAGE STABILITY
ORGANISM
UNIPROT ACCESSION NO.
LITERATURE
-10C, partially purified enzyme is stable for at least 2 months
-
Purification/COMMENTARY
ORGANISM
UNIPROT ACCESSION NO.
LITERATURE