Information on EC 2.4.2.15 - guanosine phosphorylase

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The expected taxonomic range for this enzyme is: Eukaryota

EC NUMBER
COMMENTARY
2.4.2.15
-
RECOMMENDED NAME
GeneOntology No.
guanosine phosphorylase
REACTION
REACTION DIAGRAM
COMMENTARY
ORGANISM
UNIPROT ACCESSION NO.
LITERATURE
guanosine + phosphate = guanine + alpha-D-ribose 1-phosphate
show the reaction diagram
-
-
-
-
REACTION TYPE
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
pentosyl group transfer
-
-
-
-
PATHWAY
KEGG Link
MetaCyc Link
guanine and guanosine salvage I
-
guanosine nucleotides degradation III
-
Metabolic pathways
-
Purine metabolism
-
purine ribonucleosides degradation
-
SYSTEMATIC NAME
IUBMB Comments
guanosine:phosphate alpha-D-ribosyltransferase
Also acts on deoxyguanosine.
SYNONYMS
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
phosphorylase, guanosine
-
-
-
-
CAS REGISTRY NUMBER
COMMENTARY
9030-28-8
-
ORGANISM
COMMENTARY
LITERATURE
SEQUENCE CODE
SEQUENCE DB
SOURCE
SUBSTRATE
PRODUCT                      
REACTION DIAGRAM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
(Substrate)
LITERATURE
(Substrate)
COMMENTARY
(Product)
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
deoxyguanosine + phosphate
guanine + deoxyribose 1-phosphate
show the reaction diagram
-
-
-
-
-
guanosine + phosphate
guanine + D-ribose 1-phosphate
show the reaction diagram
-
-
-
-
?
guanosine + phosphate
guanine + D-ribose 1-phosphate
show the reaction diagram
-
equilibrium constant is 0.019
-
r
guanosine + phosphate
guanine + D-ribose 1-phosphate
show the reaction diagram
-
enzyme may play an important role in the utilization by bone marrow of the corresponding preformed base and nucleoside reaching this tissue via blood
-
?
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
(Substrate)
LITERATURE
(Substrate)
COMMENTARY
(Product)
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
guanosine + phosphate
guanine + D-ribose 1-phosphate
show the reaction diagram
-
enzyme may play an important role in the utilization by bone marrow of the corresponding preformed base and nucleoside reaching this tissue via blood
-
?
INHIBITORS
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
IMAGE
PCMB
-
0.2 mM, complete inhibition
Tris buffer
-
0.1 M, pH 7.5, 22% inhibition
ACTIVATING COMPOUND
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
IMAGE
2-mercaptoethanol
-
required for optimal activity
KM VALUE [mM]
KM VALUE [mM] Maximum
SUBSTRATE
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
IMAGE
0.21
-
deoxyguanosine
-
-
0.216
-
guanosine
-
-
0.376
-
phosphate
-
reaction with guanosine
SPECIFIC ACTIVITY [µmol/min/mg]
SPECIFIC ACTIVITY MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
pH OPTIMUM
pH MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
7
7.4
-
phosphorolysis of guanosine
7
-
-
phosphorolysis of deoxyguanosine
pH RANGE
pH RANGE MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
5
8.5
-
pH 5.0: about 35% of activity maximum, pH 8.5: about 45% of activity maximum
SOURCE TISSUE
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
SOURCE
STORAGE STABILITY
ORGANISM
UNIPROT ACCESSION NO.
LITERATURE
-10 C, stable for 1 month
-
Purification/COMMENTARY
ORGANISM
UNIPROT ACCESSION NO.
LITERATURE