Any feedback?
Information on EC 2.4.2.12 - nicotinamide phosphoribosyltransferase and Organism(s) Rattus norvegicus and UniProt Accession Q80Z29
for references in articles please use BRENDA:EC2.4.2.12Please wait a moment until all data is loaded. This message will disappear when all data is loaded.
EC Tree
2.4.2.12 nicotinamide phosphoribosyltransferaseSpecify your search results
Word Map
- 2.4.2.12
- adipokines
- adiponectin
- leptin
- obesity
- adipose
-
resistin
-
adipocytokines
- nad+
-
obese
- women
- adipocytes
- mellitus
- cardiovascular
- visceral
- necrosis
-
salvage
- cholesterol
- sirt1
- endothelial
- tnf
-
homa-ir
- triglyceride
- apelin
- sirtuins
- atherosclerosis
-
c-reactive
- chemerin
- interleukin-6
-
anthropometric
-
insulin-mimetic
- ghrelin
-
circumference
- overweight
-
lean
-
omentin
-
polycystic
-
adiposity
-
waist
- vaspin
- pai-1
-
hs-crp
- c-peptide
-
obesity-related
- protein-4
-
retinol-binding
- adipsin
-
normal-weight
-
index-matched
- pharmacology
-
irisin
- medicine
- nmnats
- drug development
The taxonomic range for the selected organisms is: Rattus norvegicus
The enzyme appears in selected viruses and cellular organisms
The enzyme appears in selected viruses and cellular organisms
Reaction Schemes
Synonyms
visfatin, nampt, nicotinamide phosphoribosyltransferase, enampt, nicotinamide phosphoribosyl transferase, naprt, pre-b-cell colony-enhancing factor, inampt, nampt/visfatin, nampt/pbef/visfatin, 
more
topSYNONYM 
ORGANISM
UNIPROT
COMMENTARY 
LITERATURE
NAmPRTase
-
-
-
-
nicotinamide mononucleotide pyrophosphorylase
-
-
-
-
nicotinamide mononucleotide synthetase
-
-
-
-
NMN pyrophosphorylase
-
-
-
-
NMN synthetase
-
-
-
-
PBEF
-
-
-
-
phosphoribosyltransferase, nicotinamide
-
-
-
-
pre-B-cell colony-enhancing factor
-
-
-
-
REACTION
REACTION DIAGRAM
COMMENTARY
ORGANISM
UNIPROT
LITERATURE
nicotinamide D-ribonucleotide + diphosphate = nicotinamide + 5-phospho-alpha-D-ribose 1-diphosphate
mechanism
-
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
pentosyl group transfer
-
-
-
-
SYSTEMATIC NAME
IUBMB Comments
nicotinamide-D-ribonucleotide:diphosphate phospho-alpha-D-ribosyltransferase
-
CAS REGISTRY NUMBER
COMMENTARY
9030-27-7
-
SUBSTRATE
PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
(Substrate)
LITERATURE
(Substrate)
(Substrate)
COMMENTARY
(Product)
(Product)
LITERATURE
(Product)
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
nicotinamide + 5-phospho-alpha-D-ribose 1-diphosphate
nicotinamide D-ribonucleotide + diphosphate
-
-
-
?
nicotinamide + alpha-D-5-phosphoribosyl-1-diphosphate
nicotinamide mononucleotide + diphosphate
-
-
-
r
nicotinamide D-ribonucleotide + diphosphate
nicotinamide + 5-phospho-alpha-D-ribose 1-diphosphate
-
-
-
?
nicotinamide + 5-phospho-alpha-D-ribose 1-diphosphate
nicotinamide D-ribonucleotide + diphosphate
additional information
?
-
visfatin mimics insulin signaling by binding to the insulin receptor with an affinity similar to that of insulin and does not share the binding site with insulin on the insulin receptor
-
-
?
nicotinamide + 5-phospho-alpha-D-ribose 1-diphosphate
nicotinamide D-ribonucleotide + diphosphate
-
-
-
?
nicotinamide + 5-phospho-alpha-D-ribose 1-diphosphate
nicotinamide D-ribonucleotide + diphosphate
-
-
-
?
nicotinamide + 5-phospho-alpha-D-ribose 1-diphosphate
nicotinamide D-ribonucleotide + diphosphate
-
-
-
?
nicotinamide + 5-phospho-alpha-D-ribose 1-diphosphate
nicotinamide D-ribonucleotide + diphosphate
-
beta-nicotinamide
-
?
nicotinamide + 5-phospho-alpha-D-ribose 1-diphosphate
nicotinamide D-ribonucleotide + diphosphate
-
first step in NAD synthesis from nicotinamide
-
?
NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
(Substrate)
LITERATURE
(Substrate)
(Substrate)
COMMENTARY
(Product)
(Product)
LITERATURE
(Product)
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
nicotinamide + 5-phospho-alpha-D-ribose 1-diphosphate
nicotinamide D-ribonucleotide + diphosphate
-
-
-
?
nicotinamide + alpha-D-5-phosphoribosyl-1-diphosphate
nicotinamide mononucleotide + diphosphate
-
-
-
r
nicotinamide D-ribonucleotide + diphosphate
nicotinamide + 5-phospho-alpha-D-ribose 1-diphosphate
-
-
-
?
nicotinamide + 5-phospho-alpha-D-ribose 1-diphosphate
nicotinamide D-ribonucleotide + diphosphate
additional information
?
-
visfatin mimics insulin signaling by binding to the insulin receptor with an affinity similar to that of insulin and does not share the binding site with insulin on the insulin receptor
-
-
?
nicotinamide + 5-phospho-alpha-D-ribose 1-diphosphate
nicotinamide D-ribonucleotide + diphosphate
-
-
-
?
nicotinamide + 5-phospho-alpha-D-ribose 1-diphosphate
nicotinamide D-ribonucleotide + diphosphate
-
-
-
?
nicotinamide + 5-phospho-alpha-D-ribose 1-diphosphate
nicotinamide D-ribonucleotide + diphosphate
-
first step in NAD synthesis from nicotinamide
-
?
COFACTOR
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
FK-866
the nicotinamide-binding site is important for inhibition by FK-866
N-(4-[[3-(trifluoromethyl)phenyl]sulfonyl]benzyl)-1H-pyrazolo[3,4-b]pyridine-5-carboxamide
-
N-[(5-[[3-(trifluoromethyl)phenyl]sulfonyl]pyridin-2-yl)methyl]furo[2,3-c]pyridine-2-carboxamide
-
N-[4-[(3,5-difluorophenyl)sulfonyl]benzyl]imidazo[1,2-a]pyridine-6-carboxamide
-
NAD+
-
1 mM, 50%-90% inhibition in various tissues, at 0.2 mM not inhibitory in lung and liver
ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
pH RANGE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
7.8 - 9.2
-
about half-maximal activity at pH 7.8 and about 80% of maximal activity at pH 9.2
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
ORGANISM
COMMENTARY
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
male Wistar rats, six-months-old, develop diet-induced hyperphagia and obesity, male obese (fa/fa) Zucker rats, three-months-old, display genetic obesity due to deficit in leptin receptors, and male lean Zucker rats, three-month-old
SWissProt
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
SOURCE
similar mRNA expression levels in (fa/fa) Zucker rats (obese) compared to lean Zucker rats (control) as well as in cafeteria diet-fed Wistar rats (diet-induced obesity) compared to standard chow diet-fed Wistar rats (control)
similar mRNA expression levels in mesenteric, retroperitoneal and epididymal WAT depots of (fa/fa) Zucker rats (obese) compared to lean Zucker rats (control)
lean Wistar rats: mRNA expression levels are maximal in mesenteric WAT (2.01 +/-0.08) followed by inguinal (1.23 +/-0.15), epididymal (0.88 +/-0.13), and retroperitoneal (0.79 +/-0.07) WAT
lean Zucker rats: mRNA expression levels are similar in subcutaneous (inguinal, 1.18 +/-0.33) and visceral (mesenteric (0.99 +/-0.15), retroperitoneal (0.56 +/-0.10), epididymal (1.17 +/-0.14)) WAT
mesenteric, significantly decreased mRNA expression level in cafeteria diet-fed Wistar rats (diet-induced obesity) compared to standard chow diet-fed Wistar rats (control)
significantly reduced mRNA expression level in (fa/fa) Zucker rats (obese) compared to lean Zucker rats (control) and in cafeteria diet-fed Wistar rats (diet-induced obesity) compared to standard chow diet-fed Wistar rats (control)
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY
GeneOntology No.
LITERATURE
SOURCE
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
metabolism
enhanced enzyme expression increases cellular NAD+ concentration no more than 2fold
UNIPROT
ENTRY NAME
ORGANISM
NO. OF AA
NO. OF TRANSM. HELICES
MOLECULAR WEIGHT[Da]
SOURCE
SEQUENCE
LOCALIZATION PREDICTION
The reliability class of the localization prediction ranges from 1 (very reliable) to 5 (not reliable).
The reliability class of the localization prediction ranges from 1 (very reliable) to 5 (not reliable). NAMPT_RAT
491
0
55438
Swiss-Prot
other Location (Reliability: 1)
PDB
SCOP
CATH
UNIPROT
ORGANISM
CRYSTALLIZATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
hanging drop vapor diffusion method, crystallization of apoenzyme and complex with either nicotinamide mononucleotide or the NAmPRTase inhibitor FK-866
pH STABILITY
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
GENERAL STABILITY
ORGANISM
UNIPROT
LITERATURE
bovine serum albumin, glutathione, cysteine, DTT, EDTA, cystine or H2O2 does not stabilize during storage
-
STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
EXPRESSION
ORGANISM
UNIPROT
LITERATURE
administration of 0.1 mM salidroside alleviates Abetax1einduced cell damage and increases the expression level of the enzyme
the expression level of the enzyme decreases as a result of Abeta1-40x1einduced neuronal toxicity
REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Lin, L.F.H.; Lan, S.J.; Richardson, A.H.; Henderson, L.V.M.
Pyridine nucleotide synthesis. Purification of nicotinamide mononucleotide pyrophosphorylase from rat erythrocytes
J. Biol. Chem.
247
8016-8022
1972
Rattus norvegicus
Dietrich, L.S.; Muniz, O.
Inhibition of nicotinamide phosphoribosyltransferase by pyridine nucleotides
Biochemistry
11
1691-1695
1972
Rattus norvegicus
Shibata, K.; Taguchi, H.; Nishitani, H.; Okumura, K.; Shimabayashi, Y.; Matsushita, N.; Yamazaki, H.
End product inhibition of the activity of nicotinamide phosphoribosyltransferase from various tissues of rats by NAD
Agric. Biol. Chem.
53
2283-2284
1989
Rattus norvegicus
-
Kim, M.K.; Lee, J.H.; Kim, H.; Park, S.J.; Kim, S.H.; Kang, G.B.; Lee, Y.S.; Kim, J.B.; Kim, K.K.; Suh, S.W.; Eom, S.H.
Crystal structure of visfatin/pre-B cell colony-enhancing factor 1/nicotinamide phosphoribosyltransferase, free and in complex with the anti-cancer agent FK-866
J. Mol. Biol.
362
66-77
2006
Rattus norvegicus (Q80Z29)
Mercader, J.; Granados, N.; Caimari, A.; Oliver, P.; Bonet, M.L.; Palou, A.
Retinol-binding protein 4 and nicotinamide phosphoribosyltransferase/visfatin in rat obesity models
Horm. Metab. Res.
40
467-472
2008
Rattus norvegicus (Q80Z29), Rattus norvegicus
Zabka, T.S.; Singh, J.; Dhawan, P.; Liederer, B.M.; Oeh, J.; Kauss, M.A.; Xiao, Y.; Zak, M.; Lin, T.; McCray, B.; La, N.; Nguyen, T.; Beyer, J.; Farman, C.; Uppal, H.; Dragovich, P.S.; OBrien, T.; Sampath, D.; Misner, D.L.
Retinal toxicity, in vivo and in vitro, associated with inhibition of nicotinamide phosphoribosyltransferase
Toxicol. Sci.
144
163-172
2015
Homo sapiens (P43490), Homo sapiens, Rattus norvegicus (Q80Z29)
Huang, X.; Xing, S.; Chen, C.; Yu, Z.; Chen, J.
Salidroside protects PC12 cells from Abeta1-40-induced cytotoxicity by regulating the nicotinamide phosphoribosyltransferase signaling pathway
Mol. Med. Rep.
16
2700-2706
2017
Rattus norvegicus (Q80Z29)
Hara, N.; Osago, H.; Hiyoshi, M.; Kobayashi-Miura, M.; Tsuchiya, M.
Quantitative analysis of the effects of nicotinamide phosphoribosyltransferase induction on the rates of NAD+ synthesis and breakdown in mammalian cells using stable isotope-labeling combined with mass spectrometry
PLoS ONE
14
e0214000
2019
Homo sapiens (P43490), Rattus norvegicus (Q80Z29), Mus musculus (Q99KQ4)
EXTERNAL LINKS (specific for EC-Number 2.4.2.12)
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
