Information on EC 2.4.1.91 - flavonol 3-O-glucosyltransferase

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The expected taxonomic range for this enzyme is: Magnoliophyta

EC NUMBER
COMMENTARY
2.4.1.91
-
RECOMMENDED NAME
GeneOntology No.
flavonol 3-O-glucosyltransferase
REACTION
REACTION DIAGRAM
COMMENTARY
ORGANISM
UNIPROT ACCESSION NO.
LITERATURE
UDP-glucose + a flavonol = UDP + a flavonol 3-O-beta-D-glucoside
show the reaction diagram
-
-
-
-
UDP-glucose + a flavonol = UDP + a flavonol 3-O-beta-D-glucoside
show the reaction diagram
His22 acts as the catalytic base, and Asp121 is a key residue that may assist deprotonation of the acceptor by forming an electron transfer chain with the catalytic base, both residues, as well as Glu381, are essential in donor substrate binding and enzyme activity, acceptor substrate binding site structure
Q5IFH7
REACTION TYPE
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
hexosyl group transfer
-
-
-
-
PATHWAY
KEGG Link
MetaCyc Link
Biosynthesis of secondary metabolites
-
daphnetin modification
-
Flavone and flavonol biosynthesis
-
flavonol acylglucoside biosynthesis I - kaempferol derivatives
-
flavonol acylglucoside biosynthesis III - quercetin derivatives
-
kaempferol gentiobioside biosynthesis
-
kaempferol glycoside biosynthesis (Arabidopsis)
-
kaempferol triglucoside biosynthesis
-
Metabolic pathways
-
myricetin gentiobioside biosynthesis
-
quercetin gentiotetraside biosynthesis
-
quercetin glucoside biosynthesis (Allium)
-
quercetin glycoside biosynthesis (Arabidopsis)
-
quercetin triglucoside biosynthesis
-
rutin biosynthesis
-
SYSTEMATIC NAME
IUBMB Comments
UDP-glucose:flavonol 3-O-D-glucosyltransferase
Acts on a variety of flavonols, including quercetin and quercetin 7-O-glucoside. Different from EC 2.4.1.81 (flavone 7-O-beta-glucosyltransferase).
SYNONYMS
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
2,4,5-trichlorophenol detoxifying O-glucosyltransferase
-
-
3GT
B6DU53
-
DicGT1
Q60FF2
-
F3GT
-
-
-
-
flavonoid 3-O-glucosyltransferase
-
-
flavonoid 3-O-glucosyltransferase
-
-
flavonoid-3-O-glucosyltransferas
G8G221
-
flavonol 3-O-glucosyltransferase
C5MR71
-
flavonol 3-O-GT
C5MR71
-
glucosyltransferase, uridine diphosphoglucose-flavonol 3-O-
-
-
-
-
GT-I
-
-
-
-
RUGT-10
-
-
UDP flavonoid/triterpene GT
Q5IFH7
-
UDP glucose-flavonoid 3-O-glucosyltransferase
-
-
UDP glucose: flavonoid-3-O-glucosyltransferase
-
-
UDP glucose:flavonoid 3-O-glucosyltransferase
-
-
UDP-glucose: flavonoid 3-O-glucosyltransferase
-
-
UDP-glucose: flavonoid 3-O-glucosyltransferase
B6DU53
-
UDP-glucose: flavonoid 3-O-glucosyltransferase
A6N928
-
UDP-glucose:flavonoid 3-O-glucosyltransferase
D3Y5N8
-
UDP-glucose:flavonoid 3-O-glucosyltransferase
B5BTH5
-
UDP-glucose:flavonol 3-O-glucosyltransferase
-
-
-
-
UDPG:flavonoid-3-O-glucosyltransferase
-
-
-
-
UDPglucose:flavonoid 3-O-glycosyltransferase
-
-
UDPglucose:flavonol O3-D-glucosyltransferase
-
-
-
-
UFGT
-
-
-
-
UFGT
-
-
UGT706C1
-
-
UGT707A3
-
-
UGT71G1
Q5IFH7
-
UGT78K1
D3Y5N8
-
uridine 5'-diphosphate-glucose:flavonoid 3-O-glucosyltransferase
B5BTH5
-
VL3GT
A6N928
-
GTI
-
-
-
-
additional information
Q5IFH7
the enzyme belongs to the UGT superfamily
CAS REGISTRY NUMBER
COMMENTARY
50812-18-5
-
ORGANISM
COMMENTARY
LITERATURE
SEQUENCE CODE
SEQUENCE DB
SOURCE
cv. Red Danish
-
-
Manually annotated by BRENDA team
f. Ciliato-dentatus
-
-
Manually annotated by BRENDA team
cv. Elsanta
-
-
Manually annotated by BRENDA team
strawberry
-
-
Manually annotated by BRENDA team
cf. EC 2.4.1.115; cultivar Clark line
UniProt
Manually annotated by BRENDA team
cultivar Nuomici
-
-
Manually annotated by BRENDA team
gene RF5
-
-
Manually annotated by BRENDA team
var. Mairei
-
-
Manually annotated by BRENDA team
cultivars Luchia Lady, Aphrodite, Wedding Promenade, and I-Hsin Lucky Girl x I-Hsin Song
-
-
Manually annotated by BRENDA team
Prunus x yedoensis Matsum
Matsum
-
-
Manually annotated by BRENDA team
cv. Apeldoorn
-
-
Manually annotated by BRENDA team
Kadainou R-1, interspecific hybrid grape between Vitis ficifolia variant Ganebu and Vitis vinifera cultivar Muscat of Alexandria
UniProt
Manually annotated by BRENDA team
cultivar Concord
UniProt
Manually annotated by BRENDA team
gene VvGT1
-
-
Manually annotated by BRENDA team
GENERAL INFORMATION
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
malfunction
-
enzyme-suppressed Phalaenopsis exhibit various levels of flower color fading that is well correlated with the extent of reduced level of transcriptional activity. Furthermore, there is a significant decrease in anthocyanin content in the enzyme-suppressed Phalaenopsis flowers
metabolism
-
in anthocyanin biosynthesis, UDP-glucose: anthocyanidin 3-O-glucosyltransferase catalyzes the transfer of the glucosyl moiety from UDP-glucose to the 3-hydroxyl group of anthocyanidins, producing the first stable anthocyanins
physiological function
-
UFGT is a key enzyme for biosynthesis and stability of anthocyanin pigments of red grapes
physiological function
B5BTH5
key enzyme of the anthocyanin pathway
physiological function
-
the enzyme plays a regulatory role in anthocyanin biosynthesis in Solanum tuberosum at the transcriptional level
physiological function
-
the UDP glucose: flavonoid-3-O-glucosyltransferase gene regulates anthocyanin biosynthesis in litchi pericarp during fruit coloration
physiological function
D3Y5N8, -
the enzyme functions as a 3-O-glucosyltransferase in anthocyanin and flavonol biosynthesis in vivo
physiological function
-
the enzyme is involved in the red color formation in Phalaenopsis flowers
SUBSTRATE
PRODUCT                      
REACTION DIAGRAM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
(Substrate)
LITERATURE
(Substrate)
COMMENTARY
(Product)
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
dTDP-glucose + quercetin
dTDP + quercetin 3-O-beta-D-glucoside
show the reaction diagram
-
-
-
-
?
dTDP-xylose + quercetin
dTDP + quercetin 3-O-beta-D-xylose
show the reaction diagram
-
low activity
-
-
?
UDP-5-thio-glucose + quercetin
UDP + quercetin 3-O-5-thio-beta-D-glucoside
show the reaction diagram
-
low activity
-
-
?
UDP-alpha-D-glucose + 1-naphthol
UDP + naphthalen-1-yl beta-D-glucopyranoside
show the reaction diagram
-
glucosylated by enzyme FaGT6 and FaGT7
-
-
?
UDP-alpha-D-glucose + 2-naphthol
UDP + naphthalen-2-yl beta-D-glucopyranoside
show the reaction diagram
-
glucosylated by enzyme FaGT6 and FaGT7
-
-
?
UDP-alpha-D-glucose + 3,7-dihdroxyflavone
UDP + 3-[(beta-D-glucopyranosyl)oxy]-7-hydroxyflavone
show the reaction diagram
-
glucosylated by enzyme FaGT6 and FaGT7
-
-
?
UDP-alpha-D-glucose + 3-hydroxycoumarin
UDP + 3-[(beta-D-glucopyranosyl)oxy]-coumarin
show the reaction diagram
-
glucosylated by enzyme FaGT6 and FaGT7
-
-
?
UDP-alpha-D-glucose + 3-hydroxyflavone
UDP + 3-[(beta-D-glucopyranosyl)oxy]-flavone
show the reaction diagram
-
42% of the activity with 2,4,5-trichlorophenol
-
-
?
UDP-alpha-D-glucose + 3-hydroxyflavone
UDP + 3-[(beta-D-glucopyranosyl)oxy]-flavone
show the reaction diagram
-
glucosylated by enzyme FaGT6 and FaGT7
-
-
?
UDP-alpha-D-glucose + 6-hydroxycoumarin
UDP + 6-[(beta-D-glucopyranosyl)oxy]-coumarin
show the reaction diagram
-
glucosylated by enzyme FaGT6
-
-
-
UDP-alpha-D-glucose + 7-hydroxycoumarin
UDP + 7-[(beta-D-glucopyranosyl)oxy]-coumarin
show the reaction diagram
-
glucosylated by enzyme FaGT6 and FaGT7
-
-
?
UDP-alpha-D-glucose + cyanidin
UDP + cyanidin 3-O-beta-D-glucoside
show the reaction diagram
-
-
-
-
?
UDP-alpha-D-glucose + cyanidin
UDP + cyanidin 3-O-beta-D-glucoside
show the reaction diagram
D3Y5N8, -
-
-
-
?
UDP-alpha-D-glucose + cyanidin
UDP + cyanididin 3-O-beta-D-glucoside
show the reaction diagram
A6N928, -
this enzyme preferentially glucosylates cyanidin as compared with quercetin
-
-
?
UDP-alpha-D-glucose + daphnetin
UDP + ?
show the reaction diagram
-
glucosylated by enzyme FaGT6 and FaGT7
-
-
?
UDP-alpha-D-glucose + delphinidin
UDP + delphinidin 3-O-beta-D-glucoside
show the reaction diagram
A6N928, -
-
-
-
?
UDP-alpha-D-glucose + delphinidin
UDP + delphinidin 3-O-beta-D-glucoside
show the reaction diagram
D3Y5N8, -
best substrate
-
-
?
UDP-alpha-D-glucose + dihydroquercetin
UDP + quercetin 3-O-beta-D-glucoside
show the reaction diagram
A6N928, -
weak substrate
-
-
?
UDP-alpha-D-glucose + galangin
UDP + galangin 3-O-beta-D-glucoside
show the reaction diagram
-
glucosylated by enzyme FaGT6 and FaGT7
-
-
?
UDP-alpha-D-glucose + isorhamnetin
UDP + isorhamnetin 3-O-beta-D-glucoside
show the reaction diagram
-
-
-
-
?
UDP-alpha-D-glucose + isorhamnetin
UDP + isorhamnetin 3-O-beta-D-glucoside
show the reaction diagram
-
-
-
-
?
UDP-alpha-D-glucose + isorhamnetin
UDP + isorhamnetin 3-O-beta-D-glucoside
show the reaction diagram
-
-
-
-
?
UDP-alpha-D-glucose + isorhamnetin
UDP + isorhamnetin 3-O-beta-D-glucoside
show the reaction diagram
-
-
-
-
?
UDP-alpha-D-glucose + isorhamnetin
UDP + isorhamnetin 3-O-beta-D-glucoside
show the reaction diagram
A6N928, -
-
-
-
?
UDP-alpha-D-glucose + isorhamnetin
UDP + isorhamnetin 3-O-beta-D-glucoside
show the reaction diagram
D3Y5N8, -
-
-
-
?
UDP-alpha-D-glucose + isorhamnetin
UDP + isorhamnetin 3-O-beta-D-glucoside
show the reaction diagram
-
isorhamnetin is the best acceptor
-
-
r
UDP-alpha-D-glucose + isorhamnetin
UDP + isorhamnetin 3-O-beta-D-glucoside
show the reaction diagram
-
71% of the activity with kaempferol
-
-
?
UDP-alpha-D-glucose + isorhamnetin
UDP + isorhamnetin 3-O-beta-D-glucoside
show the reaction diagram
-
108% of the activity with kaempferol
-
-
?
UDP-alpha-D-glucose + isorhamnetin
UDP + isorhamnetin 3-O-beta-D-glucoside
show the reaction diagram
-
glucosylated by enzyme FaGT6 and FaGT7
-
-
?
UDP-alpha-D-glucose + kaempferol
UDP + kaempferol 3-O-beta-D-glucoside
show the reaction diagram
-
-
-
-
?
UDP-alpha-D-glucose + kaempferol
UDP + kaempferol 3-O-beta-D-glucoside
show the reaction diagram
-
-
-
-
?
UDP-alpha-D-glucose + kaempferol
UDP + kaempferol 3-O-beta-D-glucoside
show the reaction diagram
-
-
-
-
?
UDP-alpha-D-glucose + kaempferol
UDP + kaempferol 3-O-beta-D-glucoside
show the reaction diagram
D3Y5N8, -
-
-
-
?
UDP-alpha-D-glucose + kaempferol
UDP + kaempferol 3-O-beta-D-glucoside
show the reaction diagram
A6N928, -
weak substrate
-
-
?
UDP-alpha-D-glucose + kaempferol
UDP + kaempferol 3-O-beta-D-glucoside
show the reaction diagram
-
100% activity
-
-
?
UDP-alpha-D-glucose + kaempferol
UDP + kaempferol 3-O-beta-D-glucoside
show the reaction diagram
-
UGT706C1 and UGT707A3 preferentially use kaempferol over quercetin
-
-
?
UDP-alpha-D-glucose + kaempferol
UDP + kaempferol 3-O-beta-D-glucoside
show the reaction diagram
C5MR71, -
57.9% activity compared to quercetin, glucosylation occurs at the 3 hydroxyl position
-
-
?
UDP-alpha-D-glucose + kaempferol
UDP + kaempferol 3-O-beta-D-glucoside
show the reaction diagram
-
glucosylated by enzyme FaGT6 and FaGT7
-
-
?
UDP-alpha-D-glucose + malvidin
UDP + malvidin 3-O-beta-D-glucoside
show the reaction diagram
D3Y5N8, -
-
-
-
?
UDP-alpha-D-glucose + malvidin
UDP + malvidin 3-O-beta-D-glucoside
show the reaction diagram
A6N928, -
best substrate
-
-
?
UDP-alpha-D-glucose + malvidin
UDP + malvidin 3-O-beta-D-glucoside
show the reaction diagram
-
glucosylated by enzyme FaGT6
-
-
?
UDP-alpha-D-glucose + morin
UDP + morin 3-O-beta-D-glucoside
show the reaction diagram
-
glucosylated by enzyme FaGT6
-
-
?
UDP-alpha-D-glucose + myricetin
UDP + myricetin 3-O-beta-D-glucoside
show the reaction diagram
D3Y5N8, -
-
-
-
?
UDP-alpha-D-glucose + myricetin
UDP + myricetin 3-O-beta-D-glucoside
show the reaction diagram
-
glucosylated by enzyme FaGT6 and FaGT7
-
-
?
UDP-alpha-D-glucose + pelargonidin
UDP + pelargonidin 3-O-beta-D-glucoside
show the reaction diagram
-
-
-
-
?
UDP-alpha-D-glucose + pelargonidin
UDP + pelargonidin 3-O-beta-D-glucoside
show the reaction diagram
D3Y5N8, -
-
-
-
?
UDP-alpha-D-glucose + peonidin
UDP + peonidin 3-O-beta-D-glucoside
show the reaction diagram
A6N928, -
-
-
-
?
UDP-alpha-D-glucose + peonidin
UDP + peonidin 3-O-beta-D-glucoside
show the reaction diagram
D3Y5N8, -
-
-
-
?
UDP-alpha-D-glucose + peonidin
UDP + peonidin 3-O-beta-D-glucoside
show the reaction diagram
-
glucosylated by enzyme FaGT6
-
-
?
UDP-alpha-D-glucose + petunidin
UDP + petunidin 3-O-beta-D-glucoside
show the reaction diagram
D3Y5N8, -
-
-
-
?
UDP-alpha-D-glucose + petunidin
UDP + petunidin 3-O-beta-D-glucoside
show the reaction diagram
-
glucosylated by enzyme FaGT6
-
-
?
UDP-alpha-D-glucose + quercetin
UDP + quercetin 3-O-beta-D-glucoside
show the reaction diagram
-
-
-
-
?
UDP-alpha-D-glucose + quercetin
UDP + quercetin 3-O-beta-D-glucoside
show the reaction diagram
-
-
-
-
?
UDP-alpha-D-glucose + quercetin
UDP + quercetin 3-O-beta-D-glucoside
show the reaction diagram
-
-
-
-
?
UDP-alpha-D-glucose + quercetin
UDP + quercetin 3-O-beta-D-glucoside
show the reaction diagram
Q5IFH7
-
-
-
?
UDP-alpha-D-glucose + quercetin
UDP + quercetin 3-O-beta-D-glucoside
show the reaction diagram
-
-
-
-
?
UDP-alpha-D-glucose + quercetin
UDP + quercetin 3-O-beta-D-glucoside
show the reaction diagram
A6N928, -
-
-
-
?
UDP-alpha-D-glucose + quercetin
UDP + quercetin 3-O-beta-D-glucoside
show the reaction diagram
D3Y5N8, -
-
-
-
?
UDP-alpha-D-glucose + quercetin
UDP + quercetin 3-O-beta-D-glucoside
show the reaction diagram
-
33% of the activity with 2,4,5-trichlorophenol
-
-
?
UDP-alpha-D-glucose + quercetin
UDP + quercetin 3-O-beta-D-glucoside
show the reaction diagram
C5MR71, -
100% activity, glucosylation occurs at the 3 hydroxyl position
-
-
ir
UDP-alpha-D-glucose + quercetin
UDP + quercetin 3-O-beta-D-glucoside
show the reaction diagram
-
glucosylated by enzyme FaGT6 and FaGT7
enzyme FaGT7 produces quercetin 3-O-beta-D-glucoside and quercetin 4'-O-beta-D-glucoside in equal amounts plus small amounts of the 7-O and 3'-O-isomers
-
?
UDP-alpha-D-glucose + scopoletin
UDP + ?
show the reaction diagram
-
glucosylated by enzyme FaGT6 and FaGT7
-
-
?
UDP-alpha-D-glucose + taxifolin
UDP + taxifolin 3-O-beta-D-glucoside
show the reaction diagram
-
glucosylated by enzyme FaGT7
-
-
?
UDP-galactose + cyanidin
?
show the reaction diagram
D3Y5N8, -
galactose can be transferred from UDP-galactose to the 3-position of cyanidin with low specificity relative to the analogous glucosyltransferase reactions
-
-
?
UDP-galactose + delphinidin
?
show the reaction diagram
D3Y5N8, -
galactose can be transferred from UDP-galactose to the 3-position of delphinidin with low specificity relative to the analogous glucosyltransferase reactions
-
-
?
UDP-galactose + quercetin
UDP + quercetin 3-O-beta-D-galactoside
show the reaction diagram
-
low activity
-
-
?
UDP-glucose + 2',4'-dihydroxyflavone
UDP + ?
show the reaction diagram
-
8.3% of the activity with 2,4,5-trichlorophenol
-
-
?
UDP-glucose + 2'-hydroxyflavone
UDP + 2'-hydroxyflavone 2'-beta-D-glucoside
show the reaction diagram
-
15% of the activity with 2,4,5-trichlorophenol
-
-
?
UDP-glucose + 2,3,6-trichlorophenol
UDP + 2,3,6-trichlorophenylglucoside
show the reaction diagram
-
9% of the activity with 2,4,5-trichlorophenol
-
-
?
UDP-glucose + 2,4,5-trichlorophenol
UDP + 2,4,5-trichlorophenylglucoside
show the reaction diagram
-
-
-
-
?
UDP-glucose + 2,4,6-trichlorophenol
UDP + 2,4,6-trichlorophenylglucoside
show the reaction diagram
-
4% of the activity with 2,4,5-trichlorophenol
-
-
?
UDP-glucose + 2,4-dinitrophenol
UDP + 2,4-dinitrophenylglucoside
show the reaction diagram
-
2.4% of the activity with 2,4,5-trichlorophenol
-
-
?
UDP-glucose + 4'-hydroxyflavone
UDP + 4'-hydroxyflavone 4'-beta-D-glucoside
show the reaction diagram
-
4% of the activity with 2,4,5-trichlorophenol
-
-
?
UDP-glucose + 4-nitrophenol
UDP + 4-nitrophenylglucoside
show the reaction diagram
-
23% of the activity with 2,4,5-trichlorophenol
-
-
?
UDP-glucose + 5-hydroxyflavone
UDP + 5-hydroxyflavone 5-beta-D-glucoside
show the reaction diagram
-
2.7% of the activity with 2,4,5-trichlorophenol
-
-
?
UDP-glucose + 6-hydroxyflavone
UDP + 6-hydroxyflavone 6-beta-D-glucoside
show the reaction diagram
-
24% of the activity with 2,4,5-trichlorophenol
-
-
?
UDP-glucose + 7-hydroxyflavone
UDP + 7-hydroxyflavone 7-beta-D-glucoside
show the reaction diagram
-
4.8% of the activity with 2,4,5-trichlorophenol
-
-
?
UDP-glucose + apigenin
UDP + apigenin 3-O-glucoside
show the reaction diagram
-
8.7% of the activity with 2,4,5-trichlorophenol
-
-
?
UDP-glucose + coumestrol
UDP + coumestrol 3-O-glucoside
show the reaction diagram
-
33% of the activity with 2,4,5-trichlorophenol
-
-
?
UDP-glucose + delphinidin
UDP + delphinidin 3-O-glucoside
show the reaction diagram
-
-
-
-
?
UDP-glucose + esculetin
UDP + ?
show the reaction diagram
-
glucosylated by enzyme FaGT6 and FaGT7
-
-
?
UDP-glucose + genistein
UDP + ?
show the reaction diagram
-
7.4% of the activity with 2,4,5-trichlorophenol
-
-
?
UDP-glucose + isorhamnetin
UDP + isorhamnetin 3-O-beta-D-glucoside
show the reaction diagram
-
-
-
-
?
UDP-glucose + isosakuranetin
UDP + ?
show the reaction diagram
-
at 11.2% the activity with kaempferol
-
-
?
UDP-glucose + luteolin
UDP + ?
show the reaction diagram
-
11% of the activity with 2,4,5-trichlorophenol
-
-
?
UDP-glucose + luteolin
UDP + ?
show the reaction diagram
-
90% activity compared to kaempferol
-
-
?
UDP-glucose + myricetin
UDP + myricetin 3-O-beta-D-glucoside
show the reaction diagram
C5MR71, -
40.7% activity compared to quercetin, glucosylation occurs at the 3 hydroxyl position
-
-
?
UDP-glucose + pelargonidin
UDP + pelargonidin 3-O-glucoside
show the reaction diagram
-
at 4% the activity with kaempferol
-
-
?
UDP-glucose + pentachlorophenol
UDP + pentachlorophenylglucoside
show the reaction diagram
-
4% of the activity with 2,4,5-trichlorophenol
-
-
?
UDP-glucose + phenol
UDP + phenyglucoside
show the reaction diagram
-
2.4% of the activity with 2,4,5-trichlorophenol
-
-
?
UDP-glucose + quercetin
UDP + quercetin 3-O-beta-D-glucoside
show the reaction diagram
-
-
-
-
?
UDP-mannose + quercetin
UDP + quercetin 3-O-beta-D-mannoside
show the reaction diagram
-
very low activity
-
-
?
UDP-N-acetylglucose + quercetin
UDP + quercetin 3-O-N-acetylglucoside
show the reaction diagram
-
low activity
-
-
?
UDP-xylose + quercetin
UDP + quercetin 3-O-beta-D-xylose
show the reaction diagram
-
low activity
-
-
?
UDPgalactose + isorhamnetin
UDP + isorhamnetin 3-O-glucoside
show the reaction diagram
-
isorhamnetin is the best acceptor, 45% of the activity compared to reaction with UDPglucose
-
-
r
UDPglucose + 3'-O-methylquercetin
UDP + 3'-O-methylquercetin 3-O-glucoside
show the reaction diagram
-
28% of the activity with quercetin
-
-
?
UDPglucose + 3,5,7-trihydroxyflavanone
UDP + 3,5,7-trihydroxyflavanone 3-O-glucoside
show the reaction diagram
-
isoenzyme F3GT1: 22% of the activity with kaempferol, isoenzyme F3GT2: 12% of the activity with kaempferol
-
-
?
UDPglucose + 4,7-dihydroxyflavonol
UDP + 4,7-dihydroxyflavonol 3-O-glucoside
show the reaction diagram
-
27% of the activity with quercetin
-
-
?
UDPglucose + 5-deoxyquercetin
UDP + 5-deoxyquercetin 3-O-glucoside
show the reaction diagram
-
57% of the activity with quercetin
-
-
?
UDPglucose + 7,4'-dihydroxyflavonol
UDP + 7,4'-dihydroxyflavonol 3-O-glucoside
show the reaction diagram
-
isoenzyme F3GT1: 33% of the activity with kaempferol, isoenzyme F3GT2: 34% of the activity with kaempferol
-
-
?
UDPglucose + a flavonol
UDP + a flavonol 3-O-D-glucoside
show the reaction diagram
-
the enzyme is critical for anthocyanin biosynthesis in the grape berry
-
-
?
UDPglucose + a flavonol
UDP + a flavonol 3-O-D-glucoside
show the reaction diagram
-
the enzyme is responsible for the glycosylation of anthocyanidins to produce stable molecules in the anthocyanin biosynthetic pathway
-
-
?
UDPglucose + a flavonol
UDP + a flavonol 3-O-D-glucoside
show the reaction diagram
-
in the conversion from colorless leucoanthocyanidin to colored anthocyanidin 3-glucoside, at least two enzymes, anthocyanidin synthase and UDP-glucose:flavonoid 3-O-glucosyltransferase, are postulated to be involved
-
-
?
UDPglucose + a flavonol
UDP + a flavonol 3-O-D-glucoside
show the reaction diagram
-
enzyme is specific for flavonol glucoside biosynthesis
-
-
?
UDPglucose + a flavonol
UDP + a flavonol 3-O-D-glucoside
show the reaction diagram
-
the enzyme is involved in the flavone glycoside pathway in parsley. Enzyme activity is increased by prior illumination of the cell cultures, maximal activity is reached about 24 h after onset of illumination
-
-
?
UDPglucose + a flavonol
UDP + a flavonol 3-O-D-glucoside
show the reaction diagram
-
the principal, if not only, role of the enzyme is to glucosylate anthocyanidins in red fruit during ripening
-
-
-
UDPglucose + a flavonol
UDP + a flavonol 3-O-D-glucoside
show the reaction diagram
B5BTH5
the final and specific step for the anthocyanin biosynthesis pathway is 3-glucoside formation by UDP-glucose:flavonoid 3-O-glucosyltransferase
-
-
?
UDPglucose + a flavonol
UDP + a flavonol 3-O-D-glucoside
show the reaction diagram
Petunia x hybrida Surfinia Purple
-
the enzyme is responsible for the glycosylation of anthocyanidins to produce stable molecules in the anthocyanin biosynthetic pathway
-
-
?
UDPglucose + apigenin
UDP + apigenin 3-O-glucoside
show the reaction diagram
-
at 4.2% the activity with kaempferol
-
-
?
UDPglucose + cyanidin
UDP + cyanidin 3-O-glucoside
show the reaction diagram
-
-
-
-
?
UDPglucose + cyanidin
UDP + cyanidin 3-O-glucoside
show the reaction diagram
Q60FF2
-
-
-
?
UDPglucose + cyanidin
UDP + cyanidin 3-O-glucoside
show the reaction diagram
-
at 5% the activity with kaempferol
-
-
?
UDPglucose + dihydrokaempferol
UDP + dihydrokaempferol 3-O-glucoside
show the reaction diagram
-
isoenzyme F3GT1: 7% of the activity with kaempferol, isoenzyme F3GT2: 7% of the activity with kaempferol
-
-
?
UDPglucose + dihydrokaempferol
UDP + dihydrokaempferol 3-O-glucoside
show the reaction diagram
-
9.6% of the activity with kaempferol
-
-
?
UDPglucose + dihydroquercetin
UDP + dihydroquercetin 3-O-glucoside
show the reaction diagram
-
-
-
-
?
UDPglucose + dihydroquercetin
UDP + dihydroquercetin 3-O-glucoside
show the reaction diagram
-
isoenzyme F3GT1: 3% of the activity with kaempferol, isoenzyme F3GT2: 2% of the activity with kaempferol
-
-
?
UDPglucose + fisetin
UDP + fisetin 3-O-glucoside
show the reaction diagram
-
-
-
-
?
UDPglucose + fisetin
UDP + fisetin 3-O-glucoside
show the reaction diagram
-
-
-
-
?
UDPglucose + kaempferol
UDP + kaempferol 3-O-glucoside
show the reaction diagram
-
-
-
-
?
UDPglucose + kaempferol
UDP + kaempferol 3-O-glucoside
show the reaction diagram
-
-
-
?
UDPglucose + kaempferol
UDP + kaempferol 3-O-glucoside
show the reaction diagram
-
-
-
-
?
UDPglucose + kaempferol
UDP + kaempferol 3-O-glucoside
show the reaction diagram
-
-
-
-
?
UDPglucose + kaempferol
UDP + kaempferol 3-O-glucoside
show the reaction diagram
-
-
-
-
?
UDPglucose + kaempferol
UDP + kaempferol 3-O-glucoside
show the reaction diagram
-
-
-
-
?
UDPglucose + kaempferol
UDP + kaempferol 3-O-glucoside
show the reaction diagram
-
45% of the activity with isorhamnetin
-
-
r
UDPglucose + kaempferol
UDP + kaempferol 3-O-glucoside
show the reaction diagram
-
70% of the activity with quercetin
-
-
?
UDPglucose + kaempferol 5,7,4'-trimethyl ether
UDP + kaempferol 5,7,4'-trimethyl ether 3-O-glucoside
show the reaction diagram
-
4% of the activity with kaempferol
-
-
?
UDPglucose + kaempferol 5,7,4'-trimethyl ether
UDP + kaempferol 5,7,4'-trimethyl ether 3-O-glucoside
show the reaction diagram
-
9% of the activity with kaempferol
-
-
?
UDPglucose + kaempferol 5,7,4'-trimethyl ether
UDP + kaempferol 5,7,4'-trimethyl ether 3-O-glucoside
show the reaction diagram
-
isoenzyme F3GT1: 52% of the activity with kaempferol, isoenzyme F3GT2: 11% of the activity with kaempferol
-
-
?
UDPglucose + kaempferol 7-O-glucoside
UDP + kaempferol 3,7-O-diglucoside
show the reaction diagram
-
10% of the activity with kaempferol
-
-
?
UDPglucose + kaempferol 7-O-glucoside
UDP + kaempferol 3,7-O-diglucoside
show the reaction diagram
-
isoenzyme F3GT1: 6% of the activity with kaempferol, isoenzyme F3GT2: 5% of the activity with kaempferol
-
-
?
UDPglucose + kaempferol-4'-O-methylether
UDP + ?
show the reaction diagram
-
-
-
-
?
UDPglucose + kaempferol-4'-O-methylether
UDP + ?
show the reaction diagram
-
42% of the activity with kaempferol
-
-
?
UDPglucose + kaempferol-4'-O-methylether
UDP + ?
show the reaction diagram
-
13% of the activity with isorhamnetin
-
-
r
UDPglucose + kaempferol-4'-O-methylether
UDP + ?
show the reaction diagram
-
57% of the activity with quercetin
-
-
?
UDPglucose + malvidin
UDP + malvidin 3-O-glucoside
show the reaction diagram
-
-
-
-
?
UDPglucose + malvidin
UDP + malvidin 3-O-glucoside
show the reaction diagram
-
at 4% the activity with kaempferol
-
-
?
UDPglucose + myricetin
UDP + myrecetin 3-O-glucoside
show the reaction diagram
-
-
-
-
?
UDPglucose + myricetin
UDP + myrecetin 3-O-glucoside
show the reaction diagram
-
43% of the activity with kaempferol
-
-
?
UDPglucose + myricetin
UDP + myrecetin 3-O-glucoside
show the reaction diagram
-
20% of the activity with kaempferol
-
-
?
UDPglucose + naringenin
UDP + naringenin 3-O-glucoside
show the reaction diagram
-
at 3.2% the activity with kaempferol
-
-
?
UDPglucose + quercetin
UDP + quercetin 3-O-glucoside
show the reaction diagram
-
-
-
-
?
UDPglucose + quercetin
UDP + quercetin 3-O-glucoside
show the reaction diagram
-
-
-
r
UDPglucose + quercetin
UDP + quercetin 3-O-glucoside
show the reaction diagram
-
-
-
?
UDPglucose + quercetin
UDP + quercetin 3-O-glucoside
show the reaction diagram
-
-
-
-
?
UDPglucose + quercetin
UDP + quercetin 3-O-glucoside
show the reaction diagram
-
-
-
-
?
UDPglucose + quercetin
UDP + quercetin 3-O-glucoside
show the reaction diagram
-
-
-
-
?
UDPglucose + quercetin
UDP + quercetin 3-O-glucoside
show the reaction diagram
-
-
-
-
?
UDPglucose + quercetin
UDP + quercetin 3-O-glucoside
show the reaction diagram
Q60FF2
-
-
-
?
UDPglucose + quercetin
UDP + quercetin 3-O-glucoside
show the reaction diagram
-
93% of the activity with isorhamnetin
-
-
r
UDPglucose + quercetin
UDP + quercetin 3-O-glucoside
show the reaction diagram
-
61.2% of the activity with kaempferol
-
-
-
UDPglucose + quercetin
UDP + quercetin 3-O-glucoside
show the reaction diagram
-
76% of the activity with kaempferol
-
-
?
UDPglucose + quercetin
UDP + quercetin 3-O-glucoside
show the reaction diagram
-
95% of the activity with quercetin
-
-
?
UDPglucose + quercetin
UDP + quercetin 3-O-glucoside
show the reaction diagram
-
quercetin is the best acceptor
-
?
UDPglucose + quercetin
UDP + quercetin 3-O-glucoside
show the reaction diagram
Prunus x yedoensis Matsum
-
-
-
-
?
UDPglucose + quercetin 5-methyl ether
UDP + quercetin 5-methyl ether 3-O-glucoside
show the reaction diagram
-
7% of the activity with kaempferol
-
-
?
UDPglucose + quercetin 5-methyl ether
UDP + quercetin 5-methyl ether 3-O-glucoside
show the reaction diagram
-
isoenzyme F3GT1: 38% of the activity with kaempferol, isoenzyme F3GT2: 25% of the activity with kaempferol
-
-
?
UDPglucose + quercetin 7-O-glucoside
UDP + quercetin 3,7-O-diglucoside
show the reaction diagram
-
12% of the activity with kaempferol
-
-
?
UDPglucose + quercetin 7-O-glucoside
UDP + quercetin 3,7-O-diglucoside
show the reaction diagram
-
isoenzyme F3GT1: 6% of the activity with kaempferol, isoenzyme F3GT2: 6% of the activity with kaempferol
-
-
?
UDPglucose + rhamnetin
UDP + rhamnetin 3-O-glucoside
show the reaction diagram
-
isoenzyme F3GT1: 20% of the activity with kaempferol, isoenzyme F3GT2: 82% of the activity with kaempferol
-
-
?
UDPglucose + rhamnetin
UDP + rhamnetin 3-O-glucoside
show the reaction diagram
-
98% of the activity with kaempferol
-
-
?
UDPglucose + rhamnetin
UDP + rhamnetin 3-O-glucoside
show the reaction diagram
-
70% of the activity with kaempferol
-
-
?
UDPglucose + rutin
UDP + rutin 3-O-glucoside
show the reaction diagram
-
4% of the activity with isorhamnetin
-
-
r
GDP-glucose + quercetin
GDP + quercetin 3-O-beta-D-glucoside
show the reaction diagram
-
low activity
-
-
?
additional information
?
-
-
only UDPglucose can serve as glucosyl donor
-
-
-
additional information
?
-
-
only UDPglucose can serve as glucosyl donor
-
-
-
additional information
?
-
-
no activity observed with cinnamic acids or simple phenols
-
-
-
additional information
?
-
-
no activity with dihydroquercetin
-
-
-
additional information
?
-
-
the enzyme is involved in flavonoid biosynthesis, overview
-
-
-
additional information
?
-
-
the enzyme catalyzes the addition of glucose from a UDP-glucose donor to the 3-O position, GT1 can also harness a wide panel of different UDP-sugars: UDP-5SGlc, UDP-Xyl, UDP-Man, UDP-Gal and UDP-GlcNAc (Table I), as well as GDP-Glc and dTDP-Xyl, substrate specificity of wild-type and mutant enzymes, overview, substrate binding structure, overview
-
-
-
additional information
?
-
C5MR71, -
does not glucosylate naringenin, apigenin, luteolin, dihydroquercetin, 4'-acetoxy-7-hydroxy-6-methoxy-isoflavone, 4'-methoxy-flavonol, 3',4'-dimethoxy-flavonol, anthocyanidin, and cyanidin
-
-
-
additional information
?
-
-
FaGT6 does not glucosylate 5-hydroxyflavone, 7-hydroxyflavone, coumarin, 4-hydroxycoumarin, 7-methoxycoumarin, catechin, epicatechin, 4-coumarate, 4-hydroxybenzoate, or 4-hydroxy-2,5-dimethyl-3-(2H)-furanone. UDP-alpha-D-glucuronoate or UDP-alpha-D-galactose do not serve as donors. FaGT7 does not glucosylate 4-hydroxycoumarin, 6-hydroxycoumarin, 7-methoxycoumarin, 4-hydroxy-2,5-dimethyl-3-(2H)-furanone, pelargonidin, malvidin, catechin, naringenin, apigenin, 5-hydroxyflavone
-
-
-
additional information
?
-
D3Y5N8, -
no activity with dihydroquercetin, genistin, genistein, daidzein, catechin, epicatechin, apigeninidin, flavonol 3-O-glucosides, anthocyanidin 3-O-glucosides and cyanidin 3-O-galactoside
-
-
-
additional information
?
-
Prunus x yedoensis Matsum
-
only UDPglucose can serve as glucosyl donor
-
-
-
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
(Substrate)
LITERATURE
(Substrate)
COMMENTARY
(Product)
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
UDP-alpha-D-glucose + cyanidin
UDP + cyanidin 3-O-beta-D-glucoside
show the reaction diagram
-
-
-
-
?
UDP-alpha-D-glucose + isorhamnetin
UDP + isorhamnetin 3-O-beta-D-glucoside
show the reaction diagram
-
glucosylated by enzyme FaGT6 and FaGT7
-
-
?
UDP-alpha-D-glucose + kaempferol
UDP + kaempferol 3-O-beta-D-glucoside
show the reaction diagram
-
-
-
-
?
UDP-alpha-D-glucose + kaempferol
UDP + kaempferol 3-O-beta-D-glucoside
show the reaction diagram
-
-
-
-
?
UDP-alpha-D-glucose + kaempferol
UDP + kaempferol 3-O-beta-D-glucoside
show the reaction diagram
-
-
-
-
?
UDP-alpha-D-glucose + kaempferol
UDP + kaempferol 3-O-beta-D-glucoside
show the reaction diagram
-
glucosylated by enzyme FaGT6 and FaGT7
-
-
?
UDP-alpha-D-glucose + pelargonidin
UDP + pelargonidin 3-O-beta-D-glucoside
show the reaction diagram
-
-
-
-
?
UDP-alpha-D-glucose + quercetin
UDP + quercetin 3-O-beta-D-glucoside
show the reaction diagram
-
-
-
-
?
UDP-alpha-D-glucose + quercetin
UDP + quercetin 3-O-beta-D-glucoside
show the reaction diagram
-
-
-
-
?
UDP-alpha-D-glucose + quercetin
UDP + quercetin 3-O-beta-D-glucoside
show the reaction diagram
-
-
-
-
?
UDP-alpha-D-glucose + quercetin
UDP + quercetin 3-O-beta-D-glucoside
show the reaction diagram
Q5IFH7
-
-
-
?
UDP-alpha-D-glucose + quercetin
UDP + quercetin 3-O-beta-D-glucoside
show the reaction diagram
-
glucosylated by enzyme FaGT6 and FaGT7
enzyme FaGT7 produces quercetin 3-O-beta-D-glucoside and quercetin 4'-O-beta-D-glucoside in equal amounts plus small amounts of the 7-O and 3'-O-isomers
-
?
UDP-glucose + isorhamnetin
UDP + isorhamnetin 3-O-beta-D-glucoside
show the reaction diagram
-
-
-
-
?
UDPglucose + a flavonol
UDP + a flavonol 3-O-D-glucoside
show the reaction diagram
-
the enzyme is critical for anthocyanin biosynthesis in the grape berry
-
-
?
UDPglucose + a flavonol
UDP + a flavonol 3-O-D-glucoside
show the reaction diagram
-
the enzyme is responsible for the glycosylation of anthocyanidins to produce stable molecules in the anthocyanin biosynthetic pathway
-
-
?
UDPglucose + a flavonol
UDP + a flavonol 3-O-D-glucoside
show the reaction diagram
-
in the conversion from colorless leucoanthocyanidin to colored anthocyanidin 3-glucoside, at least two enzymes, anthocyanidin synthase and UDP-glucose:flavonoid 3-O-glucosyltransferase, are postulated to be involved
-
-
?
UDPglucose + a flavonol
UDP + a flavonol 3-O-D-glucoside
show the reaction diagram
-
enzyme is specific for flavonol glucoside biosynthesis
-
-
?
UDPglucose + a flavonol
UDP + a flavonol 3-O-D-glucoside
show the reaction diagram
-
the enzyme is involved in the flavone glycoside pathway in parsley. Enzyme activity is increased by prior illumination of the cell cultures, maximal activity is reached about 24 h after onset of illumination
-
-
?
UDPglucose + a flavonol
UDP + a flavonol 3-O-D-glucoside
show the reaction diagram
-
the principal, if not only, role of the enzyme is to glucosylate anthocyanidins in red fruit during ripening
-
-
-
UDPglucose + a flavonol
UDP + a flavonol 3-O-D-glucoside
show the reaction diagram
Petunia x hybrida Surfinia Purple
-
the enzyme is responsible for the glycosylation of anthocyanidins to produce stable molecules in the anthocyanin biosynthetic pathway
-
-
?
additional information
?
-
-
the enzyme is involved in flavonoid biosynthesis, overview
-
-
-
COFACTOR
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
IMAGE
UDP
-
dependent
METALS and IONS
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
Mg2+
-
1 mM, stimulates
Mg2+
-
10 mM, slight stimulatory effect on isoenzyme F3GT1 and F3GT2
Mg2+
C5MR71, -
119% relative activity at 1 mM
Na2SO4
C5MR71, -
118% relative activity at 1 mM
Succinic anhydride
C5MR71, -
115% relative activity at 1 mM
INHIBITORS
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
IMAGE
2,3-Butanedione
C5MR71, -
46% residual activity at 10 mM
CaCl2
-
10 mM, 17% inhibition
CaCl2
-
10 mM, 9% inhibition
CoCl2
-
1 mM, 35% inhibition
CoCl2
-
1 mM, 50% inhibition of isoenzyme F3GT1 and 25% inhibition of isoenzyme F3GT2
Cu2+
-
1 mM CuCl2, 96% inhibition
Cu2+
-
1 mM CuCl2, 93% inhibition of isoenzyme F3GT1 and 94% inhibition of isoenzyme F3GT2
Cu2+
C5MR71, -
0.03% residual activity at 10 mM
cyanidin
D3Y5N8, -
the recombinant enzyme exhibits pronounced substrate inhibition by cyanidin at 0.1 mM
dithioerythritol
-
-
dithioerythritol
-
-
EDTA
-
10 mM, slight inhibition
Fe2+
C5MR71, -
0.09% residual activity at 10 mM
-
iodoacetamide
-
-
iodoacetamide
-
-
iodoacetate
-
-
iodoacetate
-
-
KCl
-
10 mM, 17% inhibition
KCl
-
10 mM, 9-10% inhibition
Mg2+
-
1 mM MgCl2, 18% inhibition
Mn2+
-
10 mM MnCl2, 35% inhibition
N-bromo-succinimide
C5MR71, -
17% residual activity at 10 mM
Phenylmercuriacetate
-
-
Phenylmercuriacetate
-
-
Tetranitromethane
C5MR71, -
13% residual activity at 10 mM
Tris
C5MR71, -
Tris inhibits flavonol 3-O-GT as activity levels are significantly lower than corresponding buffers across its entire buffer range
UDP
C5MR71, -
6% residual activity at 10 mM
Zn2+
-
1 mM ZnCl2, 96% inhibition
Zn2+
-
1 mM ZnCl2, 93% inhibition of isoenzyme F3GT1 and 94% inhibition of isoenzyme F3GT2
Zn2+
C5MR71, -
26% residual activity at 10 mM
Mn2+
-
10 mM MnCl2, 52% inhibition of isoenzyme F3GT1 and 28% inhibition of isoenzyme F3GT2
additional information
-
enzyme is inhibited in the crude extract
-
additional information
C5MR71, -
L-cysteine, L-histidine, L-arginine, L-tryptophan, and L-tyrosine residues are important for activity; not inhibited by succinic anhydride, K+, Na+, Ca2+,Mg2+, and Mn2+
-
ACTIVATING COMPOUND
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
IMAGE
2-mercaptoethanol
-
1 mM, activation to 120%
2-mercaptoethanol
-
14 mM, activation to 157%; 14 mM, stimulates
2-mercaptoethanol
-
14 mM, activation of isoenzyme F3GT1 to 160%, activation of isoenzyme F3GT2 to 136%
2-mercaptoethanol
-
14 mM, stimulates to 134%
dithioerythritol
-
1 mM, activation to 124%
dithioerythritol
-
10 mM, activation to 215%
EDTA
-
1 mM, activation to 173%
EDTA
-
slight activation
glutathione
-
1 mM, activation to 118%
glycine-HCl
-
glycine-HCl buffer stimulates about 2fold at pH 9 in comparison with Tris-HCl buffer
Sucrose
-
5%, activation to 123%
KM VALUE [mM]
KM VALUE [mM] Maximum
SUBSTRATE
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
IMAGE
0.0106
-
2,4,5-Trichlorophenol
-
-
0.0053
-
3-hydroxyflavone
-
pH 7.0, 30C, enzyme FaGT6
0.00479
-
cyanidin
A6N928, -
in 100 mM Tris-HCl pH 8.0, at 30C
0.03
-
cyanidin
-
recombinant enzyme
0.07
-
cyanidin
-
about
0.016
-
delphinidin
-
recombinant enzyme
0.27
-
fisetin
-
-
0.0035
-
isorhamnetin
-
pH 7.0, 30C, enzyme FaGT7
0.2
-
isorhamnetin
-
-
0.00067
-
kaempferol
-
-
0.00114
-
kaempferol
-
-
0.012
-
kaempferol
-
-
0.012
-
kaempferol
C5MR71, -
apparent value, in 50 mM phosphate buffer (pH 7.5), at 40C
0.0125
-
kaempferol
-
-
0.042
-
kaempferol
-
-
0.172
-
kaempferol
-
-
0.174
-
kaempferol
D3Y5N8, -
recombinant enzyme, in 50 mM HEPES, at pH 8.0 and 30C
0.69
-
kaempferol
-
-
0.033
-
myricetin
C5MR71, -
apparent value, in 50 mM phosphate buffer (pH 7.5), at 40C
0.001
-
quercetin
-
less than 0.001 mM
0.00121
-
quercetin
-
-
0.00216
-
quercetin
A6N928, -
in 100 mM Tris-HCl pH 8.0, at 30C
0.007
-
quercetin
-
-
0.0101
-
quercetin
-
-
0.015
-
quercetin
-
recombinant enzyme
0.031
-
quercetin
-
-
0.067
-
quercetin
C5MR71, -
apparent value, in 50 mM phosphate buffer (pH 7.5), at 40C
0.07
-
quercetin
-
about
0.126
-
quercetin
-
-
0.6
-
UDP-alpha-D-glucose
-
pH 7.0, 30C, enzyme FaGT7
0.914
-
UDP-alpha-D-glucose
A6N928, -
using cyanidin as cosubstrate, in 100 mM Tris-HCl pH 8.0, at 30C
2
-
UDP-alpha-D-glucose
-
pH 7.0, 30C, enzyme FaGT6
1.67
-
UDP-D-glucose
-
-
0.0124
-
UDP-glucose
-
reaction with 2,4,5-trichlorophenol
0.0618
-
UDP-glucose
-
reaction with quercetin
0.669
-
UDP-glucose
C5MR71, -
apparent value, in 50 mM phosphate buffer (pH 7.5), at 40C
0.0098
-
UDPglucose
-
-
0.0133
-
UDPglucose
-
-
0.18
-
UDPglucose
-
-
0.3
-
UDPglucose
-
-
0.5
-
UDPglucose
-
-
1
-
UDPglucose
-
-
1.9
-
UDPglucose
-
recombinant enzyme
0.0357
-
malvidin
-
recombinant enzyme
additional information
-
additional information
-
kinetics, recombinant enzyme
-
TURNOVER NUMBER [1/s]
TURNOVER NUMBER MAXIMUM[1/s]
SUBSTRATE
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
IMAGE
0.000702
-
cyanidin
A6N928, -
in 100 mM Tris-HCl pH 8.0, at 30C
0.5955
-
kaempferol
C5MR71, -
apparent value, in 50 mM phosphate buffer (pH 7.5), at 40C
1.25
-
kaempferol
D3Y5N8, -
recombinant enzyme, in 50 mM HEPES, at pH 8.0 and 30C
0.6252
-
myricetin
C5MR71, -
apparent value, in 50 mM phosphate buffer (pH 7.5), at 40C
0.000113
-
quercetin
A6N928, -
in 100 mM Tris-HCl pH 8.0, at 30C
1.047
-
quercetin
C5MR71, -
apparent value, in 50 mM phosphate buffer (pH 7.5), at 40C
0.000247
-
UDP-alpha-D-glucose
A6N928, -
using cyanidin as cosubstrate, in 100 mM Tris-HCl pH 8.0, at 30C
1.442
-
UDP-glucose
C5MR71, -
apparent value, in 50 mM phosphate buffer (pH 7.5), at 40C
kcat/KM VALUE [1/mMs-1]
kcat/KM VALUE [1/mMs-1] Maximum
SUBSTRATE
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
IMAGE
0.146
-
cyanidin
A6N928, -
in 100 mM Tris-HCl pH 8.0, at 30C
8851
49.6
-
kaempferol
C5MR71, -
apparent value, in 50 mM phosphate buffer (pH 7.5), at 40C
11887
18.9
-
myricetin
C5MR71, -
apparent value, in 50 mM phosphate buffer (pH 7.5), at 40C
13273
0.052
-
quercetin
A6N928, -
in 100 mM Tris-HCl pH 8.0, at 30C
16073
15.6
-
quercetin
C5MR71, -
apparent value, in 50 mM phosphate buffer (pH 7.5), at 40C
16073
0.00027
-
UDP-alpha-D-glucose
A6N928, -
using cyanidin as cosubstrate, in 100 mM Tris-HCl pH 8.0, at 30C
219166
2.2
-
UDP-glucose
C5MR71, -
apparent value, in 50 mM phosphate buffer (pH 7.5), at 40C
17641
SPECIFIC ACTIVITY [µmol/min/mg]
SPECIFIC ACTIVITY MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
0.000716
-
-
activity with 2,4,5-trichlorophenol
0.00143
-
-
activity with quercetin
additional information
-
-
-
additional information
-
-
-
additional information
-
-
-
pH OPTIMUM
pH MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
7
-
-
assay at
7.2
7.3
-
assay at
7.5
-
-
in histidine-HCl buffer
7.5
-
-
Tris-HCl buffer
7.5
-
C5MR71, -
the optimum pH is 7.5 with a pronounced buffer effect noted for reactions performed in Tris-HCl buffer
7.8
-
-
assay at
8
-
-
broad pH-optimum around pH 8
8
-
-
in imidazole-HCl buffer
8
-
-
reaction with quercetin or cyanidin
8
-
-
imidazole-HCl buffer
8
-
D3Y5N8, -
the recombinant enzyme exhibits a slight buffer preference for HEPES over Tris at pH 8.0
8.5
-
-
-
pH RANGE
pH RANGE MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
4
9
-
pH 4.0: about 60% of maximal activity, pH 9.0: about 70% of maximal activity
4.5
8
-
pH 4.5: about 80% of maximal activity, pH 8.0: about 55% of maximal activity
TEMPERATURE OPTIMUM
TEMPERATURE OPTIMUM MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
28
-
-
assay at
30
-
-
assay at
30
-
D3Y5N8, -
-
37
-
-
assay at
40
50
C5MR71, -
-
pI VALUE
pI VALUE MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
5
-
-
isoelectric focusing
5.2
-
B6DU53
calculated from amino acid sequence
5.6
-
-
calculated from amino acid sequence
6.3
-
C5MR71, -
calculated from amino acid sequence
SOURCE TISSUE
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
SOURCE
-
the specific activity increases with age of the culture, reaching a maximum late in the growth cycle
Manually annotated by BRENDA team
-
gene expression in the red-skin sports seems to be the result of a mutation in this gene or a mutation in a regulatory gene controlling its expression
Manually annotated by BRENDA team
A6N928, -
pre-veraison exocarp
Manually annotated by BRENDA team
-
mRNA expression increases in the early developmental stages, reaching the maximum at the stage before flower opening
Manually annotated by BRENDA team
A6N928, -
highest expression
Manually annotated by BRENDA team
Petunia x hybrida Surfinia Purple
-
mRNA expression increases in the early developmental stages, reaching the maximum at the stage before flower opening
-
Manually annotated by BRENDA team
-
in different stage of ripening
Manually annotated by BRENDA team
D3Y5N8, -
-
Manually annotated by BRENDA team
Prunus x yedoensis Matsum
-
-
-
Manually annotated by BRENDA team
Q60FF2
DicGT1 gene is expressed strongly in stages 2 to 4 during the development of petals in which anthocyanin synthesis occurs
Manually annotated by BRENDA team
D3Y5N8, -
-
Manually annotated by BRENDA team
D3Y5N8, -
-
Manually annotated by BRENDA team
-
highest expression
Manually annotated by BRENDA team
D3Y5N8, -
-
Manually annotated by BRENDA team
-
highest expression
Manually annotated by BRENDA team
additional information
-
not expressed in tubers and rachises
Manually annotated by BRENDA team
additional information
A6N928, -
not detected in mesocarp
Manually annotated by BRENDA team
LOCALIZATION
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
GeneOntology No.
LITERATURE
SOURCE
MOLECULAR WEIGHT
MOLECULAR WEIGHT MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
40000
-
-
gel filtration
43000
-
-
gel filtration
43000
-
-
gel filtration
48000
-
-
gel filtration
49000
-
-
gel filtration
50190
-
B6DU53
calculated from amino acid sequence
51000
-
-
gel filtration
51000
-
C5MR71, -
SDS-PAGE
51200
-
C5MR71, -
calculated from amino acid sequence
59000
-
-
gel filtration
66000
-
-
SDS-PAGE
SUBUNITS
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
?
-
x * 53000, SDS-PAGE
?
-
x * 49900, calculated from amino acid sequence
?
-
x * 52000, estimated from amino acid sequence
?
D3Y5N8, -
x * 49240, calculated from amino acid sequence; x * 49800, estimated from SDS-PAGE
dimer
-
2 * 24500, SDS-PAGE
dimer
-
2 * 59000, SDS-PAGE
additional information
Q5IFH7
the structure of UGT71G1 consists of two N- and C-terminal domains with similar Rossmann-type folds and belongs to the GT-B fold, the N-terminal domain contains a central seven-stranded parallel beta sheets flanked by eight alpha helices on both sides and a small two stranded beta sheets, the C-terminal domain contains a six stranded beta sheet flanked by eight alpha helices, the two domains pack very tightly and form a deep cleft with a UDP molecule bound, structure comparisons, overview
Crystallization/COMMENTARY
ORGANISM
UNIPROT ACCESSION NO.
LITERATURE
wild-type and selenomethionine-labeled enzyme in complex with UDP and UDP-glucose, hanging drop vapour diffusion method, 5 mg/ml protein is mixed with 5 mM UDP-galactose and 5 mM quercetin at a 2:1 v/v ratio, mixed with an equal volume of reservoir solution containing 40% w/v PEG 3350, 0.2 M ammonium acetate, and 0.1 M sodium citrate, pH 5.6, equilibration over the reservoir solution at 20C, 2-5 days, X-ray diffraction structure determination and analysis at 2.0-2.6 A resolution, molecular docking
Q5IFH7
purified recombinant detagged enzyme bound to UDP-2-fluoroglucose and kaempferol, or with UDP and quercetin, hanging drop method, 4.8 mg/ml protein in 5 mM Tris (2-carboxyethyl) phosphine HCl, 10 mM TrisCl, pH 8.3, diluted 1:1 with mother liquor which contains 1822% polyethylene glycol 10 000, 0.1M Bis-Tris propane, pH 7.0, and 00.5% v/v Pluronic F-68, crystals appear within 24 h, X-ray diffraction structure determination and analysis at 2.2 A resolution
-
pH STABILITY
pH STABILITY MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
5.5
8.5
C5MR71, -
reduction to 59% maximal activity at pH 5.5 and 57% at pH 8.5
TEMPERATURE STABILITY
TEMPERATURE STABILITY MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
40
60
C5MR71, -
the enzyme is stable when preincubated for up to 10 min at 40C with a significant decrease in activity at 50C and an almost complete loss of detectable activity at 60C and beyond
GENERAL STABILITY
ORGANISM
UNIPROT ACCESSION NO.
LITERATURE
activities recorded in phosphate, MOPS, and bicine buffers are not significantly different, but assays in Tris buffer result in a reduction of enzyme activity by 51% under optimal pH conditions
C5MR71, -
at least three freeze-thaw cycles can be tolerated without apparent loss of activity
-
STORAGE STABILITY
ORGANISM
UNIPROT ACCESSION NO.
LITERATURE
-20C, 20 mM Tris-HCl, pH 7.5, 14 mM 2-mercaptoethanol, 10% glycerol, 15% loss of activity after 2 days
-
-20C, 20 mM imidazole-HCl, pH 8.0, 14 mM 2-mercaptoethanol, 10% glycerol, 35% loss of activity after 2 days, 61% loss of activity after 1 week
-
-20C or 4C, the recombinant enzyme is stable for at least 120 h
-
-80C, stable for many months
-
Purification/COMMENTARY
ORGANISM
UNIPROT ACCESSION NO.
LITERATURE
TALON metal affinity resin column chromatography, gel filtration
C5MR71, -
isoenzyme F3GT1 and F3GT2
-
affinity-purified on immobilized reduced glutathione resin
-
native enzyme from fruits, partially
-
Talon metal affinity column chromatography
D3Y5N8, -
recombinant His-tagged wild-type and mutant enzymes from Escherichia coli strain BL21(DE3) by nickel affinity chromatography, anion exchange chromatography, and gel filtration
Q5IFH7
; partial
-
glutathione Sepharose column chromatography
A6N928, -
recombinant enzyme
-
recombinant His-tagged enzyme from Escherichia coli to homogeneity, the His-tag is cleaved off
-
Cloned/COMMENTARY
ORGANISM
UNIPROT ACCESSION NO.
LITERATURE
a binary vector containing an UDPglucose:flavonoid-3-O-glucosyltransferase cDNA under the control of the cauliflower mosaic virus 35S promoter is used to transform Eustoma grandiflorum Grise. Of four independent transgenic lines recovered, one produces high levels of the UDPglucose:flavonoid-3-O-glucosyltransferase transcript and synthesizes 3-O-glucosylated anthocyanins novel to Eusoma grandiflorum, as well as enhanced levels of 3-O-glucosylated flavonols
-
expressed in Escherichia coli BL21(DE3)RIL cells
C5MR71, -
expression in Escherichia coli
Q60FF2
expressed in Escherichia coli BL21
-
expressed in Escherichia coli BL21(DE3) pLysS cells and in a Arabidopsis thaliana mutant (ugt78d2) deficient in anthocyanidin and flavonol 3-O-glucosyltransferase activity
D3Y5N8, -
expression of His-tagged wild-type and mutant enzymes in Escherichia coli strain BL21(DE3), selenomethionine-labeled enzyme in Escherichia coli strain B834(DE3)
Q5IFH7
expressed in Escherichia coli
-
expressed in Escherichia coli BL21 (DE3) pLysS cells
-
gene RF5, functional expression in Escherichia coli
-
expression in Escherichia coli
-
expressed in Escherichia coli DH5alpha cells
-
expressed in Escherichia coli DE3 pLys S cells
A6N928, -
gene VvGT1, expression of His-tagged enzyme in Escherichia coli
-
EXPRESSION
ORGANISM
UNIPROT ACCESSION NO.
LITERATURE
the enzyme is expressed not only at the sites of anthocyanin biosynthesis, but also at sites of flavonol glycoside biosynthesis
D3Y5N8, -
enzyme gene expression is detected only after the beginning of coloring stage or yellow stage, and is highest in the red pericarps
-
transcriptional activity is higher expressed in the red color of Phalaenopsis cultivars than in the white cultivars. In the red labellum of Phalaenopsis, the enzyme also shows higher expression levels than that in the white perianth
-
enzyme expression is induced correspondingly by exogenous elicitors including gibberellic acid (about 2.5fold increased expression at 1 mg/l) and sucrose (about 5fold increased expression at 120 g/l)
-
while there is little enzyme expression before veraison, these transcripts begin to accumulate at week 8 and 10 after flowering to reach maximal levels at week 12 after flowering and decreases thereafter
A6N928, -
treatment of grape berries with 1-methylcyclopropene inhibits ufgt mRNA accumulation
-
ethylene better stimulates expression of the ufgt promoter in the dark than under light
-
ENGINEERING
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
D121A
Q5IFH7
site-directed mutagenesis, inactive mutant
D121N
Q5IFH7
site-directed mutagenesis, inactive mutant
E381A
Q5IFH7
site-directed mutagenesis, inactive mutant
H22A
Q5IFH7
site-directed mutagenesis, inactive mutant
D374A
-
site-directed mutagenesis, inactive mutant
H20A
-
site-directed mutagenesis, inactive mutant
Q375H
-
site-directed mutagenesis, the mutant shows reduced activity compared to the wild-type enzyme
Q375N
-
site-directed mutagenesis, the mutant shows reduced activity compared to the wild-type enzyme
Q375N/T141A
-
site-directed mutagenesis, the mutant shows reduced activity compared to the wild-type enzyme
T141A
-
site-directed mutagenesis, the mutant shows reduced activity compared to the wild-type enzyme