Information on EC 2.4.1.91 - flavonol 3-O-glucosyltransferase

Please wait a moment until all data is loaded. This message will disappear when all data is loaded.
Specify your search results
Mark a special word or phrase in this record:
Select one or more organisms in this record:
Show additional data
Do not include text mining results
Include (text mining) results (more...)
Include results (AMENDA + additional results, but less precise; more...)


The expected taxonomic range for this enzyme is: Magnoliophyta

EC NUMBER
COMMENTARY
2.4.1.91
-
RECOMMENDED NAME
GeneOntology No.
flavonol 3-O-glucosyltransferase
REACTION
REACTION DIAGRAM
COMMENTARY
ORGANISM
UNIPROT ACCESSION NO.
LITERATURE
UDP-glucose + a flavonol = UDP + a flavonol 3-O-beta-D-glucoside
show the reaction diagram
-
-
-
-
UDP-glucose + a flavonol = UDP + a flavonol 3-O-beta-D-glucoside
show the reaction diagram
His22 acts as the catalytic base, and Asp121 is a key residue that may assist deprotonation of the acceptor by forming an electron transfer chain with the catalytic base, both residues, as well as Glu381, are essential in donor substrate binding and enzyme activity, acceptor substrate binding site structure
Q5IFH7
REACTION TYPE
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
hexosyl group transfer
-
-
-
-
PATHWAY
KEGG Link
MetaCyc Link
Biosynthesis of secondary metabolites
-
daphnetin modification
-
Flavone and flavonol biosynthesis
-
flavonol acylglucoside biosynthesis I - kaempferol derivatives
-
flavonol acylglucoside biosynthesis III - quercetin derivatives
-
kaempferol gentiobioside biosynthesis
-
kaempferol glycoside biosynthesis (Arabidopsis)
-
kaempferol triglucoside biosynthesis
-
Metabolic pathways
-
myricetin gentiobioside biosynthesis
-
quercetin gentiotetraside biosynthesis
-
quercetin glucoside biosynthesis (Allium)
-
quercetin glycoside biosynthesis (Arabidopsis)
-
quercetin triglucoside biosynthesis
-
rutin biosynthesis
-
SYSTEMATIC NAME
IUBMB Comments
UDP-glucose:flavonol 3-O-D-glucosyltransferase
Acts on a variety of flavonols, including quercetin and quercetin 7-O-glucoside. Different from EC 2.4.1.81 (flavone 7-O-beta-glucosyltransferase).
SYNONYMS
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
2,4,5-trichlorophenol detoxifying O-glucosyltransferase
-
-
3GT
B6DU53
-
DicGT1
Q60FF2
-
F3GT
-
-
-
-
flavonoid 3-O-glucosyltransferase
-
-
flavonoid 3-O-glucosyltransferase
-
-
flavonoid-3-O-glucosyltransferas
G8G221
-
flavonol 3-O-glucosyltransferase
C5MR71
-
flavonol 3-O-GT
C5MR71
-
glucosyltransferase, uridine diphosphoglucose-flavonol 3-O-
-
-
-
-
GT-I
-
-
-
-
RUGT-10
-
-
UDP flavonoid/triterpene GT
Q5IFH7
-
UDP glucose-flavonoid 3-O-glucosyltransferase
-
-
UDP glucose: flavonoid-3-O-glucosyltransferase
-
-
UDP glucose:flavonoid 3-O-glucosyltransferase
-
-
UDP-glucose: flavonoid 3-O-glucosyltransferase
-
-
UDP-glucose: flavonoid 3-O-glucosyltransferase
B6DU53
-
UDP-glucose: flavonoid 3-O-glucosyltransferase
A6N928
-
UDP-glucose:flavonoid 3-O-glucosyltransferase
D3Y5N8
-
UDP-glucose:flavonoid 3-O-glucosyltransferase
B5BTH5
-
UDP-glucose:flavonol 3-O-glucosyltransferase
-
-
-
-
UDPG:flavonoid-3-O-glucosyltransferase
-
-
-
-
UDPglucose:flavonoid 3-O-glycosyltransferase
-
-
UDPglucose:flavonol O3-D-glucosyltransferase
-
-
-
-
UFGT
-
-
-
-
UFGT
-
-
UGT706C1
-
-
UGT707A3
-
-
UGT71G1
Q5IFH7
-
UGT78K1
D3Y5N8
-
uridine 5'-diphosphate-glucose:flavonoid 3-O-glucosyltransferase
B5BTH5
-
VL3GT
A6N928
-
GTI
-
-
-
-
additional information
Q5IFH7
the enzyme belongs to the UGT superfamily
CAS REGISTRY NUMBER
COMMENTARY
50812-18-5
-
ORGANISM
COMMENTARY
LITERATURE
SEQUENCE CODE
SEQUENCE DB
SOURCE
cv. Red Danish
-
-
Manually annotated by BRENDA team
f. Ciliato-dentatus
-
-
Manually annotated by BRENDA team
cv. Elsanta
-
-
Manually annotated by BRENDA team
strawberry
-
-
Manually annotated by BRENDA team
cf. EC 2.4.1.115; cultivar Clark line
UniProt
Manually annotated by BRENDA team
cultivar Nuomici
-
-
Manually annotated by BRENDA team
gene RF5
-
-
Manually annotated by BRENDA team
var. Mairei
-
-
Manually annotated by BRENDA team
cultivars Luchia Lady, Aphrodite, Wedding Promenade, and I-Hsin Lucky Girl x I-Hsin Song
-
-
Manually annotated by BRENDA team
Prunus x yedoensis Matsum
Matsum
-
-
Manually annotated by BRENDA team
cv. Apeldoorn
-
-
Manually annotated by BRENDA team
Kadainou R-1, interspecific hybrid grape between Vitis ficifolia variant Ganebu and Vitis vinifera cultivar Muscat of Alexandria
UniProt
Manually annotated by BRENDA team
cultivar Concord
UniProt
Manually annotated by BRENDA team
gene VvGT1
-
-
Manually annotated by BRENDA team
GENERAL INFORMATION
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
malfunction
-
enzyme-suppressed Phalaenopsis exhibit various levels of flower color fading that is well correlated with the extent of reduced level of transcriptional activity. Furthermore, there is a significant decrease in anthocyanin content in the enzyme-suppressed Phalaenopsis flowers
metabolism
-
in anthocyanin biosynthesis, UDP-glucose: anthocyanidin 3-O-glucosyltransferase catalyzes the transfer of the glucosyl moiety from UDP-glucose to the 3-hydroxyl group of anthocyanidins, producing the first stable anthocyanins
physiological function
-
UFGT is a key enzyme for biosynthesis and stability of anthocyanin pigments of red grapes
physiological function
B5BTH5
key enzyme of the anthocyanin pathway
physiological function
-
the enzyme plays a regulatory role in anthocyanin biosynthesis in Solanum tuberosum at the transcriptional level
physiological function
-
the UDP glucose: flavonoid-3-O-glucosyltransferase gene regulates anthocyanin biosynthesis in litchi pericarp during fruit coloration
physiological function
D3Y5N8, -
the enzyme functions as a 3-O-glucosyltransferase in anthocyanin and flavonol biosynthesis in vivo
physiological function
-
the enzyme is involved in the red color formation in Phalaenopsis flowers
METALS and IONS
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
Mg2+
-
1 mM, stimulates
Mg2+
-
10 mM, slight stimulatory effect on isoenzyme F3GT1 and F3GT2
Mg2+
C5MR71, -
119% relative activity at 1 mM
Na2SO4
C5MR71, -
118% relative activity at 1 mM
Succinic anhydride
C5MR71, -
115% relative activity at 1 mM
ACTIVATING COMPOUND
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
IMAGE
2-mercaptoethanol
-
1 mM, activation to 120%
2-mercaptoethanol
-
14 mM, activation to 157%; 14 mM, stimulates
2-mercaptoethanol
-
14 mM, activation of isoenzyme F3GT1 to 160%, activation of isoenzyme F3GT2 to 136%
2-mercaptoethanol
-
14 mM, stimulates to 134%
dithioerythritol
-
1 mM, activation to 124%
dithioerythritol
-
10 mM, activation to 215%
EDTA
-
1 mM, activation to 173%
EDTA
-
slight activation
glutathione
-
1 mM, activation to 118%
glycine-HCl
-
glycine-HCl buffer stimulates about 2fold at pH 9 in comparison with Tris-HCl buffer
Sucrose
-
5%, activation to 123%
TURNOVER NUMBER [1/s]
TURNOVER NUMBER MAXIMUM[1/s]
SUBSTRATE
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
IMAGE
0.000702
-
cyanidin
A6N928, -
in 100 mM Tris-HCl pH 8.0, at 30C
0.5955
-
kaempferol
C5MR71, -
apparent value, in 50 mM phosphate buffer (pH 7.5), at 40C
1.25
-
kaempferol
D3Y5N8, -
recombinant enzyme, in 50 mM HEPES, at pH 8.0 and 30C
0.6252
-
myricetin
C5MR71, -
apparent value, in 50 mM phosphate buffer (pH 7.5), at 40C
0.000113
-
quercetin
A6N928, -
in 100 mM Tris-HCl pH 8.0, at 30C
1.047
-
quercetin
C5MR71, -
apparent value, in 50 mM phosphate buffer (pH 7.5), at 40C
0.000247
-
UDP-alpha-D-glucose
A6N928, -
using cyanidin as cosubstrate, in 100 mM Tris-HCl pH 8.0, at 30C
1.442
-
UDP-glucose
C5MR71, -
apparent value, in 50 mM phosphate buffer (pH 7.5), at 40C
kcat/KM VALUE [1/mMs-1]
kcat/KM VALUE [1/mMs-1] Maximum
SUBSTRATE
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
IMAGE
0.146
-
cyanidin
A6N928, -
in 100 mM Tris-HCl pH 8.0, at 30C
8851
49.6
-
kaempferol
C5MR71, -
apparent value, in 50 mM phosphate buffer (pH 7.5), at 40C
11887
18.9
-
myricetin
C5MR71, -
apparent value, in 50 mM phosphate buffer (pH 7.5), at 40C
13273
0.052
-
quercetin
A6N928, -
in 100 mM Tris-HCl pH 8.0, at 30C
16073
15.6
-
quercetin
C5MR71, -
apparent value, in 50 mM phosphate buffer (pH 7.5), at 40C
16073
0.00027
-
UDP-alpha-D-glucose
A6N928, -
using cyanidin as cosubstrate, in 100 mM Tris-HCl pH 8.0, at 30C
219166
2.2
-
UDP-glucose
C5MR71, -
apparent value, in 50 mM phosphate buffer (pH 7.5), at 40C
17641
pH RANGE
pH RANGE MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
4
9
-
pH 4.0: about 60% of maximal activity, pH 9.0: about 70% of maximal activity
4.5
8
-
pH 4.5: about 80% of maximal activity, pH 8.0: about 55% of maximal activity
LOCALIZATION
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
GeneOntology No.
LITERATURE
SOURCE
pH STABILITY
pH STABILITY MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
5.5
8.5
C5MR71, -
reduction to 59% maximal activity at pH 5.5 and 57% at pH 8.5
TEMPERATURE STABILITY
TEMPERATURE STABILITY MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
40
60
C5MR71, -
the enzyme is stable when preincubated for up to 10 min at 40C with a significant decrease in activity at 50C and an almost complete loss of detectable activity at 60C and beyond
EXPRESSION
ORGANISM
UNIPROT ACCESSION NO.
LITERATURE
the enzyme is expressed not only at the sites of anthocyanin biosynthesis, but also at sites of flavonol glycoside biosynthesis
D3Y5N8, -
enzyme gene expression is detected only after the beginning of coloring stage or yellow stage, and is highest in the red pericarps
-
transcriptional activity is higher expressed in the red color of Phalaenopsis cultivars than in the white cultivars. In the red labellum of Phalaenopsis, the enzyme also shows higher expression levels than that in the white perianth
-
enzyme expression is induced correspondingly by exogenous elicitors including gibberellic acid (about 2.5fold increased expression at 1 mg/l) and sucrose (about 5fold increased expression at 120 g/l)
-
while there is little enzyme expression before veraison, these transcripts begin to accumulate at week 8 and 10 after flowering to reach maximal levels at week 12 after flowering and decreases thereafter
A6N928, -
treatment of grape berries with 1-methylcyclopropene inhibits ufgt mRNA accumulation
-
ethylene better stimulates expression of the ufgt promoter in the dark than under light
-