Information on EC 2.4.1.88 - globoside alpha-N-acetylgalactosaminyltransferase

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The expected taxonomic range for this enzyme is: Eutheria

EC NUMBER
COMMENTARY hide
2.4.1.88
-
RECOMMENDED NAME
GeneOntology No.
globoside alpha-N-acetylgalactosaminyltransferase
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
UDP-N-acetyl-alpha-D-galactosamine + N-acetyl-beta-D-galactosaminyl-(1->3)-alpha-D-galactosyl-(1->4)-beta-D-galactosyl-(1->4)-beta-D-glucosyl-(1<->1)-ceramide = UDP + N-acetyl-alpha-D-galactosaminyl-(1->3)-N-acetyl-beta-D-galactosaminyl-(1->3)-alpha-D-galactosyl-(1->4)-beta-D-galactosyl-(1->4)-beta-D-glucosyl-(1<->1)-ceramide
show the reaction diagram
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
hexosyl group transfer
-
-
-
-
PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
Glycosphingolipid biosynthesis - globo series
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-
Metabolic pathways
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-
SYSTEMATIC NAME
IUBMB Comments
UDP-N-acetyl-D-galactosamine:N-acetyl-D-galactosaminyl-(1->3)-D-galactosyl-(1->4)-D-galactosyl-(1->4)-D-glucosyl-(1<->1)-ceramide alpha-N-acetyl-D-galactosaminyltransferase
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CAS REGISTRY NUMBER
COMMENTARY hide
52037-97-5
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ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
-
-
-
Manually annotated by BRENDA team
no activity in Homo sapiens
due to natively occurring inactive gene mutant
-
-
Manually annotated by BRENDA team
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
UDP-N-acetyl-D-galactosamine + N-acetyl-D-galactosaminyl-1,3-D-galactosyl-1,4-D-galactosyl-1,4-D-glucosylceramide
UDP + N-acetyl-D-galactosaminyl-1,3-N-acetyl-D-galactosaminyl-1,3-D-galactosyl-1,4-D-galactosyl-1,4-D-glucosylceramide
show the reaction diagram
UDP-N-acetyl-D-galactosamine + N-acetyl-D-galactosaminyl-1,3-D-galactosyl-R
UDP + N-acetyl-D-galactosaminyl-1,3-N-acetyl-D-galactosaminyl-1,3-D-galactosyl-R
show the reaction diagram
UDP-N-acetyl-D-galactosamine + N-acetylgalactosaminyl-1,3-beta-galactosyl-alpha-1,3-galactosyl-beta-1,4-glucosylceramide
UDP + N-acetyl-D-galactosamine-1,3-N-acetylgalactosaminyl-1,3-beta-galactosyl-alpha-1,3-galactosyl-beta-1,4-glucosylceramide
show the reaction diagram
-
-
-
-
?
UDP-N-acetyl-D-galactosamine + oligosaccharide of globoside
?
show the reaction diagram
-
-
-
-
?
additional information
?
-
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
UDP-N-acetyl-D-galactosamine + N-acetyl-D-galactosaminyl-1,3-D-galactosyl-1,4-D-galactosyl-1,4-D-glucosylceramide
UDP + N-acetyl-D-galactosaminyl-1,3-N-acetyl-D-galactosaminyl-1,3-D-galactosyl-1,4-D-galactosyl-1,4-D-glucosylceramide
show the reaction diagram
UDP-N-acetyl-D-galactosamine + N-acetyl-D-galactosaminyl-1,3-D-galactosyl-R
UDP + N-acetyl-D-galactosaminyl-1,3-N-acetyl-D-galactosaminyl-1,3-D-galactosyl-R
show the reaction diagram
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production of the Forssman antigen
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-
?
additional information
?
-
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Co2+
-
can replace Mn2+ with 20% efficiency, inhibits in the presence of Mn2+
Cu2+
-
can replace Mn2+ with 31% efficiency, inhibits in the presence of Mn2+
Fe2+
-
can replace Mn2+ with 76% efficiency
Zn2+
-
can replace Mn2+ with 83% efficiency
additional information
-
no activation by Ni2+
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
Co2+
-
in the presence of Mn2+
Cu2+
-
in the presence of Mn2+
Ni2+
-
in the presence of Mn2+
Sodium deoxycholate
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Triton CF-54
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Tween 20
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Tween 80
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-
UDP-galactose
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UDP-glucose
UDP-N-acetylglucosamine
uridine
Zn2+
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in the presence of Mn2+
additional information
ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
ATP
-
slightly activating
diphosphatidylglycerol
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activation
GSH
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slight stimulation
phosphatidic acid
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activation
phosphatidylcholine
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activation
phosphatidylethanolamine
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activation
phosphatidylinositol
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activation
phosphatidylserine
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activation
sodium taurocholate
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activation
sphingomyelin
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activation
Triton X-100
UDP-glucose
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slight stimulation
additional information
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requirement for phospholipids
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KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.00156 - 0.5
globoside
0.01 - 0.1
UDP-N-acetylgalactosamine
Ki VALUE [mM]
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.033
UDP-glucose
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SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
0.000059
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purified enzyme
additional information
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
6.5
assay at
6.9
-
assay at
pH RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
-
low activity
Manually annotated by BRENDA team
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low activity
Manually annotated by BRENDA team
additional information
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
120000
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gel filtration
additional information
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
dimer
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1 * 56000 + 1 * 60000, SDS-PAGE
additional information
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enzyme structure modeling
Crystallization/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
structure determination and molecular modeling on the basis of crystal structure of the bovine alpha3GalT, Protein Data Bank code 1K4V
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GENERAL STABILITY
ORGANISM
UNIPROT
LITERATURE
glycerol, 50%, stabilizes during storage
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phosphatidylserine stabilizes
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repeated freeze-thawing inactivates, UDP and glycerol protect
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UDP, 0.1 mM, stabilizes during storage
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STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
-20°C, at least 2 weeks without loss of activity
-
-80°C, 0.1 mg/ml enzyme in 25 mM sodium cacodylate buffer with 1% Triton X-100, at least 6 months
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4°C, 0.1 mg/ml enzyme in 25 mM sodium cacodylate buffer with 1% Triton X-100, 40% loss of activity within 3 months
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Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
affinity chromatography
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Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
cloned from cDNA library by PCR, expression, transcription and processing, of wild-type in COS-1 cells with and without HA-tag, but no detectable enzyme activity in the transfected cells by recombinant enzyme, DNA and amino acid sequence determination and analysis
cloned in a eukaryotic expression vector and transfected into CHO cells; cloned in a eukaryotic expression vector and transfected into CHO cells
DNA and amino acid sequence determination and analysis, stable expression in and functional complementation of Vero cells, which renders the cells resistant against the toxin
expression in COS-1 cells with and without HA-tag; expression of wild-type in T42 human bladder and Vero monkey kidney epithelial cells, enables the cells to bind a canine Escherichia coli uropathogen, which they cannot bind without transfection
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expression in COS-1 cells, DNA and amino acid sequence determination
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Gb4-synthase, expression in 1B9 mouse fibroblast L cells, coexpression of SV 40 large T antigen for extrachromosomal replication of the transfected plasmids
ENGINEERING
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
additional information