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Information on EC 2.4.1.82 - galactinol-sucrose galactosyltransferase and Organism(s) Pisum sativum and UniProt Accession Q8VWN6

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EC Tree
     2 Transferases
         2.4 Glycosyltransferases
             2.4.1 Hexosyltransferases
                2.4.1.82 galactinol-sucrose galactosyltransferase
IUBMB Comments
4-Nitrophenyl alpha-D-galactopyranoside can also act as donor. The enzyme also catalyses an exchange reaction between raffinose and sucrose (cf. EC 2.4.1.123, inositol 3-alpha-galactosyltransferase).
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This record set is specific for:
Pisum sativum
UNIPROT: Q8VWN6
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Word Map
The taxonomic range for the selected organisms is: Pisum sativum
The expected taxonomic range for this enzyme is: Eukaryota, Bacteria
Synonyms
raffinose synthase, zmrafs, raffinose synthase 2, galactinol-sucrose galactosyltransferase, raffinose synthase 5, raffinose synthase 4, raffinose synthase 3, raffinose synthase 6, more
SYNONYM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
raffinose synthase
-
galactosyltransferase, galactinol-sucrose
-
-
-
-
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
alpha-D-galactosyl-(1->3)-1D-myo-inositol + sucrose = myo-inositol + raffinose
show the reaction diagram
ping-pong reaction mechanism
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
hexosyl group transfer
-
-
-
-
PATHWAY SOURCE
PATHWAYS
-
-, -, -
SYSTEMATIC NAME
IUBMB Comments
alpha-D-galactosyl-(1->3)-1D-myo-inositol:sucrose 6-alpha-D-galactosyltransferase
4-Nitrophenyl alpha-D-galactopyranoside can also act as donor. The enzyme also catalyses an exchange reaction between raffinose and sucrose (cf. EC 2.4.1.123, inositol 3-alpha-galactosyltransferase).
CAS REGISTRY NUMBER
COMMENTARY hide
62213-45-0
-
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
1-alpha-D-galactosyl-myo-inositol + D-ononitol
myo-inositol + alpha-D-galactosyl-D-ononitol
show the reaction diagram
D-ononitol is 1-D-4-O-methyl-myo-inositol
-
r
1-alpha-D-galactosyl-myo-inositol + D-pinitol
myo-inositol + galactopinitol A
show the reaction diagram
D-pinitol is 1-D-3-O-methyl-chiro-inositol
i.e. O-alpha-D-galactopyranosyl-(1,2)-4-O-methyl-D-chiro-inositol
r
1-alpha-D-galactosyl-myo-inositol + H2O
myo-inositol + D-galactose
show the reaction diagram
hydrolysis, in the absence of acceptor substrate
-
-
?
1-alpha-D-galactosyl-myo-inositol + sucrose
myo-inositol + raffinose
show the reaction diagram
-
-
r
4-nitrophenol-alpha-D-galactopyranoside + sucrose
4-nitrophenol + raffinose
show the reaction diagram
-
-
-
?
alpha-D-galactosyl-(1->3)-1D-myo-inositol + sucrose
myo-inositol + raffinose
show the reaction diagram
-
-
-
?
alpha-D-galactosyl-D-ononitol + sucrose
D-ononitol + raffinose
show the reaction diagram
-
-
?
additional information
?
-
NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
alpha-D-galactosyl-(1->3)-1D-myo-inositol + sucrose
myo-inositol + raffinose
show the reaction diagram
-
-
-
?
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
1-deoxygalactonojirimycin
strong, competitive towards galactinol
myo-inositol
competitive against sucrose
Ni2+
destabilization, 30.2% activity at 1 mM
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
1 - 7.9
galactinol
22.6 - 22.9
sucrose
Ki VALUE [mM]
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.189
1-deoxygalactonojirimycin
-
10.1 - 22.3
myo-inositol
3.7
sucrose
hydrolysis reaction of galactinol, noncompetitive against galactinol
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
0.00005
recombinant in Sf21 insect cells
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
maturing
Manually annotated by BRENDA team
additional information
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
additional information
UNIPROT
ENTRY NAME
ORGANISM
NO. OF AA
NO. OF TRANSM. HELICES
MOLECULAR WEIGHT[Da]
SOURCE
SEQUENCE
LOCALIZATION PREDICTION?
RFS_PEA
798
0
88717
Swiss-Prot
Chloroplast (Reliability: 5)
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
88700
amino acid sequence determination
PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
no success in purifying the recombinant His-tagged enzyme from Sf21 insect cells
CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
functional expression as His-tagged protein in Spodoptera frugiperda Sf21 cells via baculovirus infection
gene RFS, cloned from genomic DNA from seddling leaves, DNA and amino acid sequence determination and analysis, semi-quantitative PCR enzyme expression analysis
REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Peterbauer, T.; Mach, L.; Mucha, J.; Richter, A.
Functional expression of a cDNA encoding pea (Pisum sativum L.) raffinose synthase, partial purification of the enzyme from maturing seeds, and steady-state kinetic analysis of raffinose synthesis
Planta
215
839-846
2002
Pisum sativum (Q8VWN6), Pisum sativum
Manually annotated by BRENDA team
Lahuta, L.B.; Pluskota, W.E.; Stelmaszewska, J.; Szablinska, J.
Dehydration induces expression of GALACTINOL SYNTHASE and RAFFINOSE SYNTHASE in seedlings of pea (Pisum sativum L.)
J. Plant Physiol.
171
1306-1314
2014
Pisum sativum (Q8VWN6), Pisum sativum, Pisum sativum Ramzes (Q8VWN6)
Manually annotated by BRENDA team