Information on EC 2.4.1.82 - galactinol-sucrose galactosyltransferase

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The expected taxonomic range for this enzyme is: Magnoliophyta

EC NUMBER
COMMENTARY hide
2.4.1.82
-
RECOMMENDED NAME
GeneOntology No.
galactinol-sucrose galactosyltransferase
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
alpha-D-galactosyl-(1->3)-1D-myo-inositol + sucrose = myo-inositol + raffinose
show the reaction diagram
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
hexosyl group transfer
PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
Galactose metabolism
-
-
lychnose and isolychnose biosynthesis
-
-
stachyose biosynthesis
-
-
stellariose and mediose biosynthesis
-
-
SYSTEMATIC NAME
IUBMB Comments
alpha-D-galactosyl-(1->3)-1D-myo-inositol:sucrose 6-alpha-D-galactosyltransferase
4-Nitrophenyl alpha-D-galactopyranoside can also act as donor. The enzyme also catalyses an exchange reaction between raffinose and sucrose (cf. EC 2.4.1.123, inositol 3-alpha-galactosyltransferase).
CAS REGISTRY NUMBER
COMMENTARY hide
62213-45-0
-
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
; plants are cold-treated
-
-
Manually annotated by BRENDA team
cultivar Guonong No. 25
UniProt
Manually annotated by BRENDA team
L. cv. Bravo
-
-
Manually annotated by BRENDA team
-
UniProt
Manually annotated by BRENDA team
v. California Dark Red
-
-
Manually annotated by BRENDA team
cv. Wunder von Kelvedon
SwissProt
Manually annotated by BRENDA team
; plants are cold-treated
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
physiological function
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
1-alpha-D-galactosyl-myo-inositol + D-ononitol
myo-inositol + alpha-D-galactosyl-D-ononitol
show the reaction diagram
D-ononitol is 1-D-4-O-methyl-myo-inositol
-
r
1-alpha-D-galactosyl-myo-inositol + D-pinitol
myo-inositol + galactopinitol A
show the reaction diagram
D-pinitol is 1-D-3-O-methyl-chiro-inositol
i.e. O-alpha-D-galactopyranosyl-(1,2)-4-O-methyl-D-chiro-inositol
r
1-alpha-D-galactosyl-myo-inositol + H2O
myo-inositol + D-galactose
show the reaction diagram
1-alpha-D-galactosyl-myo-inositol + sucrose
myo-inositol + raffinose
show the reaction diagram
4-nitrophenol-alpha-D-galactopyranoside + sucrose
4-nitrophenol + raffinose
show the reaction diagram
alpha-D-galactosyl-(1->3)-1D-myo-inositol + sucrose
myo-inositol + raffinose
show the reaction diagram
-
-
-
?
alpha-D-galactosyl-D-ononitol + sucrose
D-ononitol + raffinose
show the reaction diagram
-
-
?
galactinol + sucrose
myo-inositol + raffinose
show the reaction diagram
-
-
-
ir
galactinol + sucrose
raffinose + ?
show the reaction diagram
-
-
-
?
myo-inositol + raffinose
1-alpha-D-galactosyl-1D-myo-inositol + sucrose
show the reaction diagram
raffinose + sucrose
sucrose + raffinose
show the reaction diagram
additional information
?
-
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
1-alpha-D-galactosyl-myo-inositol + sucrose
myo-inositol + raffinose
show the reaction diagram
-
first step in biosynthesis of raffinose sugars
-
-
-
alpha-D-galactosyl-(1->3)-1D-myo-inositol + sucrose
myo-inositol + raffinose
show the reaction diagram
Q6ZLJ9
-
-
-
?
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
1-deoxygalactonojirimycin
strong, competitive towards galactinol
Ag+
-
1 mM, strong
Al3+
-
1 mM, strong
Hg2+
-
1 mM, strong
iodoacetamide
-
49% inhibition at 1 mM
Mn2+
-
-
myo-inositol
competitive against sucrose
N-ethylmaleimide
-
90% inhibition at 1 mM
Ni2+
destabilization, 30.2% activity at 1 mM
Zn2+
-
1 mM, strong
ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
heat shock transcription factor A2
in HsfA2 overexpressing plants transcription levels of RS2 increases 5fold
-
additional information
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
1 - 7.9
galactinol
10
raffinose
-
+ sucrose, exchange reaction
1 - 22.9
Sucrose
Ki VALUE [mM]
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.189
1-deoxygalactonojirimycin
-
10.1 - 22.3
myo-inositol
3.7
Sucrose
hydrolysis reaction of galactinol, noncompetitive against galactinol
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
0.00005
recombinant in Sf21 insect cells
0.0012
-
crude seed extract
0.00138
-
protoplast
0.00258
-
protoplast
0.00396
-
protoplast
0.497
-
purified enzyme
additional information
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
6 - 7
-
-
pH RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
5.7 - 7.8
-
about half-maximal activity at pH 5.7 and pH 7.8
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
TEMPERATURE RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
28 - 50
-
about half-maximal activity at 28C and 50C
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
80000
-
gel filtration
88700
amino acid sequence determination
100000
-
glycerol density gradient centrifugation
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
?
x * 79000, calculated from amino acid sequence
TEMPERATURE STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
50
-
10 min, 80% loss of activity, galactinol and raffinose protect, not sucrose
GENERAL STABILITY
ORGANISM
UNIPROT
LITERATURE
dithioerythritol stabilizes during purification
-
STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
4C, in crude extract, t1/2: 3 days
-
frozen, purified enzyme, at least 1 month
-
Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
no success in purifying the recombinant His-tagged enzyme from Sf21 insect cells; partial
partial
Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
expressed in Nicotiana tabacum cultivar Yunyan 85
functional expression as His-tagged protein in Spodoptera frugiperda Sf21 cells via baculovirus infection
EXPRESSION
ORGANISM
UNIPROT
LITERATURE
enzyme expression can be induced by low temperature (highest expression after 2 h at 6C) and 0.15 mM (fruit) and 0.2 mM (leaf) abscisic acid
gene expression of Os07g0209100 encoding raffinose synthase is upregulated by overexpression of the transcription factor 11 (WRKY11) induced by heat pretreatment
ENGINEERING
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
S150F
line 165, mutation induced by mutagen ethyl-methanesulfonate, seed phenotype does not differ from wild-type
T107I
line 397, mutation induced by mutagen ethyl-methanesulfonate, seed sucrose is increased by 28%, raffinose is reduced to 37% and stachyose is reduced to approximately 24% compared to wild-type