Information on EC 2.4.1.81 - flavone 7-O-beta-glucosyltransferase

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The expected taxonomic range for this enzyme is: Gunneridae

SplaateEC_Number,Commentary
EC NUMBER
COMMENTARY
2.4.1.81
-
SplaateRecommended_Name,GO_Number
RECOMMENDED NAME
GeneOntology No.
flavone 7-O-beta-glucosyltransferase
-
SplaateReaction,Reaction_id,Commentary,IF(Commentary != '',Organism,'') ,IF(Commentary != '',Literature,'')
REACTION
REACTION DIAGRAM
COMMENTARY
ORGANISM
UNIPROT
LITERATURE
UDP-glucose + 5,7,3',4'-tetrahydroxyflavone = UDP + 7-O-beta-D-glucosyl-5,7,3',4'-tetrahydroxyflavone
show the reaction diagram
-
-
-
-
SplaateReaction_Type,Organism,Commentary,Literature
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
hexosyl group transfer
-
-
-
-
SplaatePathway,BRENDA_Link,KEGG_Link,MetaCyc_Link,Source_Database
PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
apigenin glycosides biosynthesis
-
-
luteolin glycosides biosynthesis
-
-
Flavone and flavonol biosynthesis
-
-
SplaateSystematic_Name,Commentary_IUBMB
SYSTEMATIC NAME
IUBMB Comments
UDP-glucose:5,7,3',4'-tetrahydroxyflavone 7-O-beta-D-glucosyltransferase
A number of flavones, flavanones and flavonols can function as acceptors. Different from EC 2.4.1.91 (flavonol 3-O-glucosyltransferase).
SplaateSynonyms,Organism,Commentary,Literature
SYNONYMS
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
glucosyltransferase, uridine diphosphoglucose-apigenin 7-O-
-
-
-
-
glucosyltransferase, uridine diphosphoglucose-luteolin
-
-
-
-
UDP-glucose-apigenin beta-glucosyltransferase
-
-
-
-
UDP-glucosyltransferase
-
-
-
-
UDPglucose-luteolin beta-D-glucosyltransferase
-
-
-
-
uridine diphosphoglucose-apigenin 7-O-glucosyltransferase
-
-
-
-
SplaateCAS_Registry_Number,Commentary
CAS REGISTRY NUMBER
COMMENTARY
37332-50-6
-
SplaateOrganism, Commentary,Literature, Sequence_Code,Sequence_db,Textmining
ORGANISM
COMMENTARY
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
SplaateGeneral_Information, Organism, Commentary, Literature
SplaateSubstrates,Products,id,Organism_Substrates,Commentary_Substrates, Literature_Substrates, Commentary_Products, Literature_Products,Reversibility
SUBSTRATE
PRODUCT                      
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
LITERATURE
(Substrate)
COMMENTARY
(Product)
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
TDP-glucose + luteolin
TDP + 7-O-beta-D-glucosylluteolin
show the reaction diagram
-
-
-
?
UDP-alpha-D-glucose + kaempferol
UDP + kaempferol 7-O-beta-D-glucoside
show the reaction diagram
78.9% of the activity with eriodictyol
-
?
UDP-alpha-D-glucose + quercetin
UDP + quercetin 7-O-beta-D-glucoside
show the reaction diagram
64.1% of the activity with eriodictyol
-
?
UDP-glucose + 3',4',5,7-tetrahydroxyflavone
UDP + 7-O-beta-D-glucosyl-3',4',5,7-tetrahydroxyflavone
show the reaction diagram
-
involved in biosynthesis of flavone glucosides
-
?
UDP-glucose + eriodictyol
UDP + 7-O-beta-D-glucosyl-eriodictyol
show the reaction diagram
-
-
?
UDP-glucose + luteolin
UDP + 7-O-beta-D-glucosyl-luteolin
show the reaction diagram
92.1% of the activity with eriodictyol
-
?
UDP-glucose + naringenin
UDP + 7-O-beta-D-glucosyl-naringenin
show the reaction diagram
71.6% of the activity with eriodictyol
-
?
UDPglucose + 3,7,4'-trihydroxyflavone
UDP + 7-O-beta-D-glucosyl-3,7,4'trihydroxyflavone
show the reaction diagram
-
9% of the activity with apigenin
-
?
UDPglucose + 5,7-dihydroxyflavanone
UDP + 7-O-beta-D-glucosyl-5,7-dihydroxyflavanone
show the reaction diagram
-
15% of the activity with apigenin
-
?
UDPglucose + 7,4'-dihydroxyflavone
UDP + 7-O-beta-D-glucosyl-7,4'-dihydroxyflavone
show the reaction diagram
-
20% of the activity with apigenin
-
?
UDPglucose + acacetin
UDP + 7-O-beta-D-glucosylacacetin
show the reaction diagram
-
24% of the activity with apigenin
-
?
UDPglucose + apigenin
UDP + 7-O-beta-D-glucosylluteolin
show the reaction diagram
-
-
-
?
UDPglucose + apigenin
UDP + 7-O-beta-D-glucosyl-apigenin
show the reaction diagram
29.6% of the activity with eriodictyol
-
?
UDPglucose + chrysoeriol
UDP + 7-O-beta-D-glucosylchrysoeriol
show the reaction diagram
-
63% of the activity with apigenin
-
?
UDPglucose + datiscetin
UDP + 7-O-beta-D-glucosyldatiscetin
show the reaction diagram
-
9% of the activity with apigenin
-
?
UDPglucose + fisetin
UDP + 7-O-beta-D-glucosylfisetin
show the reaction diagram
-
14% of the activity with apigenin
-
?
UDPglucose + galangin
UDP + 7-O-beta-D-glucosylgalangin
show the reaction diagram
-
9% of the activity with apigenin
-
?
UDPglucose + kaempferid
UDP + 7-O-beta-D-glucosylkaempferid
show the reaction diagram
-
16% of the activity with apigenin
-
?
UDPglucose + kaempferol
UDP + 7-O-beta-D-glucosylkaempferol
show the reaction diagram
-
11% of the activity with apigenin
-
?
UDPglucose + luteolin
UDP + 7-O-beta-D-glucosylluteolin
show the reaction diagram
-
210% of the activity with apigenin
-
?
UDPglucose + naringenin
UDP + 7-O-beta-D-glucosylnaringenin
show the reaction diagram
-
45% of the activity with apigenin
-
?
UDPglucose + quercetin
UDP + 7-O-beta-D-glucosylquercetin
show the reaction diagram
-
16% of the activity with apigenin
-
?
SplaateNatural_Substrates,Natural_Products,id,Organism_Substrates,Commentary_Substrates,Literature_Substrates,Commentary_Products,Literature_Products,Reversibility
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
LITERATURE
(Substrate)
COMMENTARY
(Product)
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
UDP-glucose + 3',4',5,7-tetrahydroxyflavone
UDP + 7-O-beta-D-glucosyl-3',4',5,7-tetrahydroxyflavone
show the reaction diagram
-
involved in biosynthesis of flavone glucosides
-
?
SplaateCofactor,Organism,Commentary,Literature,Filename
SplaateMetals_Ions,Organism,Commentary, Literature
SplaateInhibitors, Organism, Commentary, Literature,Filename
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
(NH4)2SO4
-
weak
ethylene monomethylether
-
-
Tris-HCl
-
in 1 M Tris-HCl buffer the reaction rate is 70% of that observed in 0.1 M buffer
SplaateActivating_Compound, Organism, Commentary, Literature,Filename
ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
bovine serum albumin
-
stimulates
-
SplaateKM_Value,KM_Value_Maximum, Substrate,Organism, Commentary, Literature, Filename
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
0.0027
apigenin
-
-
0.0018
eriodictyol
pH 7.0
0.0263
kaempferol
pH 7.0
0.0015
luteolin
-
-
0.01
Naringenin
-
-
0.0353
quercetin
pH 7.0
0.26
TDPglucose
-
reaction with apigenin
0.12
UDPglucose
-
reaction with apigenin
SplaateTurnover_Number, Turnover_Number_Maximum, Substrate,Organism,Commentary, Literature, Filename
SplaateKCat_KM_Value,KCat_KM_Value_Maximum, Substrate,Organism, Commentary, Literature, Filename
SplaateKI_Value,KI_Value_Maximum, Inhibitor,Organism, Commentary, Literature, Filename
SplaateIC50_Value,IC50_Value_Maximum, Inhibitor,Organism, Commentary, Literature, Filename
SplaateSpecific_Activity, Specific_Activity_Maximum, Organism ,Commentary, Literature
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
SplaatepH_Optimum, pH_Optimum_Maximum, Organism, Commentary, Literature
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
SplaatepH_Range,pH_Range_Maximum, Organism,Commentary, Literature
pH RANGE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
6 - 9
-
pH 6.0: about 35% of maximal activity, pH 9.0: about 50% of maximal activity
SplaateTemperature_Optimum, Temperature_Optimum_Maximum, Organism, Commentary, Literature
SplaateTemperature_Range, Temperature_Range_Maximum, Organism, Commentary, Literature
SplaatepI_Value,pI_Value_Maximum, Organism,Commentary, Literature
SplaateSource_Tissue, Organism, Commentary, Literature, Textmining
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
SOURCE
-
extractable activity is drastically increased by illuminating the cells with white light and the maximum activity is reached 24 h after the onset of illumination
Manually annotated by BRENDA team
SplaateLocalization, Organism, Commentary, id_go, Literature, Textmining
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY
GeneOntology No.
LITERATURE
SOURCE
SplaatePDB,PDB,PDB,Organism,Uniprot_ID
SplaateMolecular_Weight, Molecular_Weight_Maximum, Organism, Commentary, Literature
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
50000
-
gel filtration
489425
SplaateSubunits, Organism, Commentary, Literature
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
?
x * 54800, calculated from sequence
SplaatePosttranslational_Modification, Organism, Commentary, Literature
SplaateCommentary, Organism, Literature
SplaatepH_Stability,pH_Stability_Maximum, Organism, Commentary, Literature
pH STABILITY
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
6.5
-
rapid loss of activity below
489425
7.5 - 8.5
-
most stable
489425
9.5
-
above, rapid loss of activity above
489425
SplaateTemperature_Stability,Temperature_Stability_Maximum, Organism, Commentary, Literature
SplaateGeneral_Stability, Organism, Literature
SplaateOrganic_Solvent, Organism, Commentary, Literature
SplaateOxidation_Stability,Organism,Literature
SplaateStorage_Stability, Organism, Literature
STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
3°C, t1/2: 1 month in 0.05 M Tris-HCl buffer, pH 7.5, 7 mM 2-mercaptoethanol
-
SplaateCommentary, Organism, Literature
Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
SplaateCommentary, Organism, Literature
Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
expression in Escherichia coli as a glutathione S-transferase fusion protein
SplaateCommentary, Organism, Literature
SplaateEngineering, Organism, Commentary, Literature
SplaateCommentary, Organism, Literature
SplaateApplication,Organism,Commentary,Literature
APPLICATION
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
synthesis
biotransformation of eriodictyol using Escherichi coli expressing AtGT-2