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Information on EC 2.4.1.69 - type 1 galactoside alpha-(1,2)-fucosyltransferase and Organism(s) Rattus norvegicus and UniProt Accession Q10980

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EC Tree
     2 Transferases
         2.4 Glycosyltransferases
             2.4.1 Hexosyltransferases
                2.4.1.69 type 1 galactoside alpha-(1,2)-fucosyltransferase
IUBMB Comments
The enzyme acts on a glycoconjugates where R (see reaction) is a glycoprotein or glycosphingolipid. The recognized moiety of the substrate is known as a type 1 histo-blood group antigen precursor disaccharide, and the action of the enzyme produces an H type 1 antigen. In humans the main enzyme performing this reaction is encoded by the FUT2 gene (also known as the Secretor gene), which is also able to act on type 2 substrates (see EC 2.4.1.344). The enzyme from the bacterium Helicobacter pylori cannot act on type 2 substrates.
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Rattus norvegicus
UNIPROT: Q10980
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The taxonomic range for the selected organisms is: Rattus norvegicus
The expected taxonomic range for this enzyme is: Eukaryota, Bacteria
Synonyms
fucosyltransferase, alpha1,2-fucosyltransferase, alpha(1,2)fucosyltransferase, fucosyltransferase 2, alpha1,2-ft, alpha(1,2)-fucosyltransferase, alpha-2-l-fucosyltransferase, fucosyltransferase 1, alpha-1,2-fucosyltransferase, alpha1,2ft, more
SYNONYM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
alpha-2-fucosyltransferase
-
alpha1,2fucosyltransferase
-
galactoside 2-alpha-L-fucosyltransferase 1
-
alpha-(1->2)-L-fucosyltransferase
-
-
-
-
alpha-2-fucosyltransferase
alpha-2-L-fucosyltransferase
-
-
-
-
alpha-L-fucosyltransferase
-
-
-
-
alpha1,2FT
-
-
-
-
alpha1,2fucosyltransferase
-
alpha1-2FucT
-
-
-
-
beta-galactoside alpha1-2fucosyltransferase
-
-
-
-
blood group H alpha-2-fucosyltransferase
-
-
-
-
blood-group substance H-dependent fucosyltransferase
-
-
-
-
fucosyltransferase, guanosine diphosphofucose-galactoside 2-L-
-
-
-
-
fucosyltransferase, guanosine diphosphofucose-glycoprotein 2-alpha-
-
-
-
-
fucosyltransferase, guanosine diphosphofucose-lactose
-
-
-
-
FucT
-
-
-
-
galactoside 2-alpha-L-fucosyltransferase
-
-
-
-
galactoside 2-alpha-L-fucosyltransferase 2
-
GDP fucose-lactose fucosyltransferase
-
-
-
-
GDP-beta-L-fucose:beta-D-galactosyl-R 2-alpha-L-fucosyltransferase
-
-
-
-
GDP-fucose:GM1 alpha1-2fucosyltransferase
-
-
-
-
GDP-L-fucose:beta-D-galactosyl-R-alpha-L-fucosyltransferase
-
-
-
-
GDP-L-fucose:lactose fucosyltransferase
-
-
-
-
GDPFuc:beta-D-galactoside alpha1,2-fucosyltransferase
-
-
-
-
guanosine diphospho-L-fucose-lactose fucosyltransferase
-
-
-
-
guanosine diphosphofucose-beta-D-galactosyl-alpha-2-L-fucosyltransferase
-
-
-
-
guanosine diphosphofucose-galactosylacetylglucosaminylgalactosylglucosylceramide alpha-L-fucosyltransferase
-
-
-
-
guanosine diphosphofucose-glycoprotein 2-alpha-L-fucosyltransferase
-
-
-
-
H gene-encoded beta-galactoside alpha1->2 fucosyltransferase
-
-
-
-
H-gene-encoded beta-galactoside alpha1-2fucosyltransferase
-
-
-
-
Se gene-encoded beta-galactoside alpha1->2 fucosyltransferase
-
-
-
-
secretor-type beta-galactoside alpha1-2fucosyltransferase
-
-
-
-
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
hexosyl group transfer
-
-
-
-
SYSTEMATIC NAME
IUBMB Comments
GDP-beta-L-fucose:beta-D-galactosyl-(1->3)-N-acetyl-beta-D-glucosaminyl-R alpha-(1,2)-L-fucosyltransferase (configuration-inverting)
The enzyme acts on a glycoconjugates where R (see reaction) is a glycoprotein or glycosphingolipid. The recognized moiety of the substrate is known as a type 1 histo-blood group antigen precursor disaccharide, and the action of the enzyme produces an H type 1 antigen. In humans the main enzyme performing this reaction is encoded by the FUT2 gene (also known as the Secretor gene), which is also able to act on type 2 substrates (see EC 2.4.1.344). The enzyme from the bacterium Helicobacter pylori cannot act on type 2 substrates.
CAS REGISTRY NUMBER
COMMENTARY hide
56093-23-3
-
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
GDP-beta-L-fucose + beta-D-galactosyl-(1->3)-N-acetyl-beta-D-galactosamine
GDP + alpha-L-fucosyl-(1->2)-beta-D-galactosyl-(1->3)-N-acetyl-beta-D-galactosamine
show the reaction diagram
GDP-beta-L-fucose + beta-D-galactosyl-(1->3)-N-acetyl-beta-D-glucosamine
GDP + alpha-L-fucosyl-(1->2)-beta-D-galactosyl-(1->3)-N-acetyl-beta-D-glucosamine
show the reaction diagram
GDP-beta-L-fucose + beta-D-galactosyl-(1->3)-N-acetyl-beta-D-glucosamine
GDP + alpha-L-fucosyl-(1->2)-beta-D-galactosyl-(1->3)-N-acetyl-beta-D-glucosamine
show the reaction diagram
residues of H type 1 epitopes, reaction of EC 2.4.1.69
-
-
?
GDP-beta-L-fucose + beta-D-galactosyl-(1->4)-N-acetyl-beta-D-galactosamine
GDP + alpha-L-fucosyl-(1->2)-beta-D-galactosyl-(1->4)-N-acetyl-beta-D-galactosamine
show the reaction diagram
residues of H type 3 epitopes, reaction of EC 2.4.1.69
-
-
?
GDP-beta-L-fucose + beta-D-galactosyl-(1->4)-N-acetyl-beta-D-glucosamine
GDP + alpha-L-fucosyl-(1->2)-beta-D-galactosyl-(1->4)-N-acetyl-beta-D-glucosamine
show the reaction diagram
residues of H type 2 epitopes
-
-
?
GDP-beta-L-fucose + lacto-N-biose
?
show the reaction diagram
-
-
-
?
GDP-beta-L-fucose + phenyl beta-D-galactopyranoside
GDP + phenyl alpha-L-fucosyl-(1->2)-beta-D-galactopyranoside
show the reaction diagram
-
-
-
?
GDP-L-fucose + beta-D-galactosyl-R
GDP + alpha-L-fucosyl-1,2-beta-D-galactosyl-R
show the reaction diagram
GDP-L-fucose + beta-methyl-D-galactopyranoside
GDP + alpha-L-fucosyl-1,2-beta-methyl-D-galactopyranoside
show the reaction diagram
-
-
-
-
?
GDP-L-fucose + D-Galbeta(1-2)D-Xylalpha(1-6)D-Glcbeta(1-4)D-Glc
GDP + L-Fucalpha(1-2)-D-Galbeta1-2-D-Xylalpha1-6-D-Glcbeta1-4-D-Glc
show the reaction diagram
-
-
-
?
GDP-L-fucose + fetuin
GDP + alpha-L-fucosyl-1,2-fetuin
show the reaction diagram
-
-
-
-
?
GDP-L-fucose + galacto-N-biose
GDP + alpha-L-fucosyl-1,2-galacto-N-biose
show the reaction diagram
-
-
-
?
GDP-L-fucose + Galbeta(1-2)D-Xyl
GDP + L-Fucalpha(1-2)D-Galbeta(1-2)D-Xyl
show the reaction diagram
-
-
-
?
GDP-L-fucose + intestinal glycoprotein
GDP + alpha-L-fucosyl-1,2-intestinal glycoprotein
show the reaction diagram
-
-
-
-
?
GDP-L-fucose + lacto-N-biose 1
GDP + L-Fucalpha(1-2)Galbeta(1-3)GlcNAc
show the reaction diagram
GDP-L-fucose + lacto-N-fucopentaose II
GDP + ?
show the reaction diagram
-
-
-
-
?
GDP-L-fucose + lacto-N-tetraose
GDP + lacto-N-fucopentaose I
show the reaction diagram
-
-
-
?
GDP-L-fucose + lactose
GDP + fucosyllactose
show the reaction diagram
-
-
-
-
?
GDP-L-fucose + N-acetyllactosamine
GDP + alpha-L-fucosyl-1,2-N-acetyllactosamine
show the reaction diagram
GDP-L-fucose + phenyl-beta-D-galactoside
GDP + phenyl 2-O-(alpha-L-fucopyranosyl)-beta-D-galactopyranoside
show the reaction diagram
GDP-L-fucose + porcine submaxillary mucin
GDP + alpha-L-fucosyl-1,2-porcine submaxillary mucin
show the reaction diagram
-
-
-
-
?
additional information
?
-
the enzyme shows a strong preference for terminal Galbeta1, 3GalNAc structures
-
-
?
NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
GDP-beta-L-fucose + beta-D-galactosyl-(1->3)-N-acetyl-beta-D-galactosamine
GDP + alpha-L-fucosyl-(1->2)-beta-D-galactosyl-(1->3)-N-acetyl-beta-D-galactosamine
show the reaction diagram
residues of H type 3 epitopes, reaction of EC 2.4.1.69
-
-
?
GDP-beta-L-fucose + beta-D-galactosyl-(1->3)-N-acetyl-beta-D-glucosamine
GDP + alpha-L-fucosyl-(1->2)-beta-D-galactosyl-(1->3)-N-acetyl-beta-D-glucosamine
show the reaction diagram
residues of H type 1 epitopes, reaction of EC 2.4.1.69
-
-
?
GDP-beta-L-fucose + beta-D-galactosyl-(1->3)-N-acetyl-beta-D-glucosamine
GDP + alpha-L-fucosyl-(1->2)-beta-D-galactosyl-(1->3)-N-acetyl-beta-D-glucosamine
show the reaction diagram
residues of H type 1 epitopes, reaction of EC 2.4.1.69
-
-
?
GDP-beta-L-fucose + beta-D-galactosyl-(1->4)-N-acetyl-beta-D-galactosamine
GDP + alpha-L-fucosyl-(1->2)-beta-D-galactosyl-(1->4)-N-acetyl-beta-D-galactosamine
show the reaction diagram
residues of H type 3 epitopes, reaction of EC 2.4.1.69
-
-
?
GDP-beta-L-fucose + beta-D-galactosyl-(1->4)-N-acetyl-beta-D-glucosamine
GDP + alpha-L-fucosyl-(1->2)-beta-D-galactosyl-(1->4)-N-acetyl-beta-D-glucosamine
show the reaction diagram
residues of H type 2 epitopes
-
-
?
GDP-beta-L-fucose + phenyl beta-D-galactopyranoside
GDP + phenyl alpha-L-fucosyl-(1->2)-beta-D-galactopyranoside
show the reaction diagram
-
-
-
?
GDP-L-fucose + beta-D-galactosyl-R
GDP + alpha-L-fucosyl-1,2-beta-D-galactosyl-R
show the reaction diagram
-
fucosyltransferase 1 may contribute to pathological prostatic growth
-
-
?
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Ca2+
-
soluble and membrane-bound enzyme are active in absence of added cations, but addition of 10 mM Mn2+, Mg2+ or Ca2+ stimulates
Mn2+
-
soluble and membrane-bound enzyme are active in absence of added cations, but addition of 10 mM Mn2+, Mg2+ or Ca2+ stimulates
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
2',3'-cyclic GMP
-
slight
dGDP
-
potent
GDP
-
competitive
GDPmannose
-
-
guanosine
-
slight
NEM
-
20 mM, 72% inhibition
ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
CHAPSO
the truncated enzyme is optimally activated by CHAPSO, showing little activity in absence
Triton CF-54
the native and the recombinant full-length enzyme display significant activity in absence of detergent or phospholipid and are optimally activated in presence of Triton CF-54 detergent
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
11.1
beta-D-galactosyl-(1->3)-N-acetyl-beta-D-galactosamine
pH 7.2, 37°C
7.1
beta-D-galactosyl-(1->3)-N-acetyl-beta-D-glucosamine
pH 7.2, 37°C
0.068
GDP-beta-L-fucose
pH 7.2, 37°C
3.8
beta-D-galactosyl-(1->3)-N-acetyl-beta-D-galactosamine
pH 7.2, 37°C
8.3
beta-D-galactosyl-(1->3)-N-acetyl-beta-D-glucosamine
pH 7.2, 37°C
3.8 - 11.1
galacto-N-biose
0.024
GDP-beta-L-fucose
pH 7.2, 37°C
0.0011 - 0.068
GDP-L-fucose
7.1 - 8.3
lacto-N-biose
5.2 - 20
N-acetyllactosamine
33
phenyl beta-D-galactopyranoside
pH 7.2, 37°C
25
phenyl beta-galactose
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
5.8
-
soluble enzyme
6
-
membrane-bound enzyme
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
small and large intestines
Manually annotated by BRENDA team
mRNA of FTA and FTC
Manually annotated by BRENDA team
e.g. hepatoma H35 cells, the enzyme is induced during early stages of chemical hepatocarcinogenesis in parenchymal cells of Fischer 344 rats fed a diet supplemented with 0.03% N-2-acetylaminofluorene, undetectable in normal liver tissue
Manually annotated by BRENDA team
small and large intestine
Manually annotated by BRENDA team
mRNA of FTA and FTC
Manually annotated by BRENDA team
mRNA of FTA and FTC
Manually annotated by BRENDA team
mRNA of FTA and FTC
Manually annotated by BRENDA team
mRNA of FTA, FTB and FTC
Manually annotated by BRENDA team
mRNA of FTA, FTB and FTC
Manually annotated by BRENDA team
additional information
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
physiological function
physiological function
UNIPROT
ENTRY NAME
ORGANISM
NO. OF AA
NO. OF TRANSM. HELICES
MOLECULAR WEIGHT[Da]
SOURCE
SEQUENCE
LOCALIZATION PREDICTION?
FUT1_RAT
376
1
42417
Swiss-Prot
Secretory Pathway (Reliability: 2)
PROTEIN VARIANTS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
additional information
recombinant full length enzyme has a specific activity over 80fold higher than the truncated enzyme missing the first 27 amino acid residues from the N-terminus, comprising the cytoplasmic and transmembrane domains. The native and the recombinant full-length enzyme display significant activity in absence of detergent or phospholipid and are optimally activated in presence of Triton CF-54 detergent. The truncated enzyme is optimally activated by CHAPSO, showing littel activity in absence
PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
transfection of Chinese hamster ovary cells
cloning of gene FTB and FTC
full length enzyme and truncated enzyme missing the first 27 amino acid residues from the N-terminus, comprising the cytoplasmic and transmembrane domains
transfection of Chinese hamster ovary cells
REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Bella, A.; Kim, Y.S.
Biosynthesis of intestinal glycoprotein: a study of an (1 lead to 2) fucosyltransferase in rat small intestinal mucosa
Arch. Biochem. Biophys.
147
753-761
1971
Rattus norvegicus
Manually annotated by BRENDA team
Bella, A.; Kim, Y.S.
Inhibition of rat small-intestinal alpha-(1-> 2)-fucosyltransferase
Biochem. J.
125
1157-1158
1971
Rattus norvegicus
Manually annotated by BRENDA team
Sherwood, A.L.; Stroud, M.R.; Levery, S.B.; Holmes, E.H.
An amino acid region at the N-terminus of rat hepatoma alpha1->2 fucosyltransferase modulates enzyme activity and interaction with lipids: strong preference for glycosphingolipids containing terminal Galbeta1->3GalNAc-structures
Biochemistry
40
5708-5719
2001
Rattus norvegicus (Q10984)
Manually annotated by BRENDA team
Marker, P.C.; Stephan, J.P.; Lee, J.; Bald, L.; Mather, J.P.; Cunha, G.R.
Fucosyltransferase1 and H-type complex carbohydrates modulate epithelial cell proliferation during prostatic branching morphogenesis
Dev. Biol.
233
95-108
2001
Homo sapiens, Rattus norvegicus
Manually annotated by BRENDA team
Karaivanova, V.; Mookerjea, S.; Hunt, D.; Nagpurkar, A.
Characterization and purification of fucosyltransferases from the cytosol of rat colon
Int. J. Biochem. Cell Biol.
28
165-174
1996
Rattus norvegicus
Manually annotated by BRENDA team
Druzhinina, T.N.; Sizova, O.V.; Shibaev, V.N.
Fucosylation of synthetic oligosaccharides - plant xyloglucan fragments - by mammalian and amphibian fucosyltransferases
Biochemistry (Moscow)
62
62-65
1997
Rana temporaria, Rattus norvegicus, Sus scrofa, Xenopus laevis
Manually annotated by BRENDA team
Bureau, V.; Marionneau, S.; Cailleau-Thomas, A.; Le Moullac-Vaidye, B.; Liehr, T.; Le Pendu, J.
Comparison of the three rat GDP-L-fucose:beta-D-galactoside 2-alpha-L-fucosyltransferases FTA, FTB and FTC
Eur. J. Biochem.
268
1006-1019
2001
Rattus norvegicus, Rattus norvegicus (Q10980), Rattus norvegicus (Q10984)
Manually annotated by BRENDA team
Abrantes, J.; Posada, D.; Guillon, P.; Esteves, P.J.; Le Pendu, J.
Widespread gene conversion of alpha-2-fucosyltransferase genes in mammals
J. Mol. Evol.
69
22-31
2009
Bos taurus (A4IFH1), Bos taurus (Q28113), Bos taurus (Q9TTY3), Chlorocebus sabaeus, Chlorocebus sabaeus (O77711), Chlorocebus sabaeus (O77712), Gorilla gorilla (O77486), Gorilla gorilla (Q9TUD4), Homo sapiens (P19526), Homo sapiens (Q10981), Homo sapiens (Q5XLR8), Homo sapiens, Hylobates agilis (Q6LA46), Hylobates lar (Q9TTC7), Macaca fascicularis (Q9N266), Monodelphis domestica, Mus musculus (O09160), Mus musculus (P97353), Oryctolagus cuniculus (Q10979), Oryctolagus cuniculus (Q10983), Oryctolagus cuniculus (Q29505), Oryctolagus cuniculus, Pan troglodytes (O77485), Pan troglodytes (Q9TUD6), Pongo pygmaeus (O77487), Pongo pygmaeus (Q6LA45), Rattus norvegicus (Q10980), Rattus norvegicus (Q10984), Rattus norvegicus (Q9WUE6), Sus scrofa (Q10982), Sus scrofa (Q29043), Xenopus tropicalis
Manually annotated by BRENDA team