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Information on EC 2.4.1.69 - type 1 galactoside alpha-(1,2)-fucosyltransferase and Organism(s) Homo sapiens and UniProt Accession P19526

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EC Tree
     2 Transferases
         2.4 Glycosyltransferases
             2.4.1 Hexosyltransferases
                2.4.1.69 type 1 galactoside alpha-(1,2)-fucosyltransferase
IUBMB Comments
The enzyme acts on a glycoconjugates where R (see reaction) is a glycoprotein or glycosphingolipid. The recognized moiety of the substrate is known as a type 1 histo-blood group antigen precursor disaccharide, and the action of the enzyme produces an H type 1 antigen. In humans the main enzyme performing this reaction is encoded by the FUT2 gene (also known as the Secretor gene), which is also able to act on type 2 substrates (see EC 2.4.1.344). The enzyme from the bacterium Helicobacter pylori cannot act on type 2 substrates.
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This record set is specific for:
Homo sapiens
UNIPROT: P19526
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Word Map
The taxonomic range for the selected organisms is: Homo sapiens
The expected taxonomic range for this enzyme is: Eukaryota, Bacteria
Synonyms
fucosyltransferase, alpha1,2-fucosyltransferase, alpha(1,2)fucosyltransferase, fucosyltransferase 2, alpha1,2-ft, alpha(1,2)-fucosyltransferase, alpha-2-l-fucosyltransferase, fucosyltransferase 1, alpha-1,2-fucosyltransferase, alpha1,2ft, more
SYNONYM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
alpha 1, 2-fucosyltransferase 1
-
alpha(1,2)-fucosyltransferase
-
alpha-2-fucosyltransferase
-
alpha1,2-fucosyltransferase
-
alpha1,2fucosyltransferase
-
fucosyltransferase 1
-
alpha (1,2)fucosyltransferase
-
-
alpha (1,2)fucosyltransferase 2
-
-
alpha (1,2)fucosyltransferase I
-
-
alpha(1,2)-fucosyltransferase-I
-
-
alpha(1,2)fucosyltransferase I
-
-
alpha-(1,2)-fucosyltransferase
-
-
alpha-(1->2)-L-fucosyltransferase
-
-
-
-
alpha-1,2-fucosyltransferase
-
-
alpha-2-fucosyltransferase
alpha-2-L-fucosyltransferase
-
-
-
-
alpha-L-fucosyltransferase
-
-
-
-
alpha1,2-FucT
-
-
alpha1,2FT
-
-
-
-
alpha1,2fucosyltransferase
-
alpha1-2FucT
-
-
-
-
beta-galactoside alpha1-2fucosyltransferase
-
-
-
-
blood group H alpha-2-fucosyltransferase
-
-
-
-
blood-group substance H-dependent fucosyltransferase
-
-
-
-
fucosyltransferase
-
fucosyltransferase 2
fucosyltransferase, guanosine diphosphofucose-galactoside 2-L-
-
-
-
-
fucosyltransferase, guanosine diphosphofucose-glycoprotein 2-alpha-
-
-
-
-
fucosyltransferase, guanosine diphosphofucose-lactose
-
-
-
-
FucT
-
-
-
-
futC
-
-
galactoside 2-alpha-L-fucosyltransferase
-
-
-
-
GDP fucose-lactose fucosyltransferase
-
-
-
-
GDP-beta-L-fucose:beta-D-galactosyl-R 2-alpha-L-fucosyltransferase
-
-
-
-
GDP-fucose:GM1 alpha1-2fucosyltransferase
-
-
-
-
GDP-L-fucose:beta-D-galactosyl-R-alpha-L-fucosyltransferase
-
-
-
-
GDP-L-fucose:lactose fucosyltransferase
-
-
-
-
GDPFuc:beta-D-galactoside alpha1,2-fucosyltransferase
-
-
-
-
guanosine diphospho-L-fucose-lactose fucosyltransferase
-
-
-
-
guanosine diphosphofucose-beta-D-galactosyl-alpha-2-L-fucosyltransferase
-
-
-
-
guanosine diphosphofucose-galactosylacetylglucosaminylgalactosylglucosylceramide alpha-L-fucosyltransferase
-
-
-
-
guanosine diphosphofucose-glycoprotein 2-alpha-L-fucosyltransferase
-
-
-
-
H gene-encoded beta-galactoside alpha1->2 fucosyltransferase
-
-
-
-
H-gene-encoded beta-galactoside alpha1-2fucosyltransferase
-
-
-
-
Se gene-encoded beta-galactoside alpha1->2 fucosyltransferase
-
-
-
-
secretor-type beta-galactoside alpha1-2fucosyltransferase
-
-
-
-
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
hexosyl group transfer
-
-
-
-
SYSTEMATIC NAME
IUBMB Comments
GDP-beta-L-fucose:beta-D-galactosyl-(1->3)-N-acetyl-beta-D-glucosaminyl-R alpha-(1,2)-L-fucosyltransferase (configuration-inverting)
The enzyme acts on a glycoconjugates where R (see reaction) is a glycoprotein or glycosphingolipid. The recognized moiety of the substrate is known as a type 1 histo-blood group antigen precursor disaccharide, and the action of the enzyme produces an H type 1 antigen. In humans the main enzyme performing this reaction is encoded by the FUT2 gene (also known as the Secretor gene), which is also able to act on type 2 substrates (see EC 2.4.1.344). The enzyme from the bacterium Helicobacter pylori cannot act on type 2 substrates.
CAS REGISTRY NUMBER
COMMENTARY hide
56093-23-3
-
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
GDP-beta-L-fucose + beta-D-galactosyl-(1->3)-N-acetyl-beta-D-glucosaminyl-(1->3)-beta-D-galactosyl-(1->4)-beta-D-glucosyl-(1<->1)-ceramide
GDP + alpha-L-fucosyl-(1->2)-beta-D-galactosyl-(1->3)-N-acetyl-beta-D-glucosaminyl-(1->3)-beta-D-galactosyl-(1->4)-beta-D-glucosyl-(1<->1)-ceramide
show the reaction diagram
-
-
-
?
GDP-beta-L-fucose + 2-nitrophenyl beta-D-galactopyranoside
GDP + 2-nitrophenyl alpha-L-fucosyl-(1->2)-beta-D-galactopyranoside
show the reaction diagram
-
-
-
-
?
GDP-beta-L-fucose + beta-D-galactose
?
show the reaction diagram
-
-
-
-
?
GDP-beta-L-fucose + beta-D-galactosyl-(1->3)-N-acetyl-D-glucosamine
GDP + alpha-L-fucosyl-(1->2)-beta-D-galactosyl-(1->3)-N-acetyl-D-glucosamine
show the reaction diagram
GDP-beta-L-fucose + beta-D-galactosyl-(1->4)-N-acetyl-D-glucosamine
GDP + alpha-L-fucosyl-(1->2)-beta-D-galactosyl-(1->4)-N-acetyl-D-glucosamine
show the reaction diagram
-
i.e. N-acetyllactosamine, reaction of EC 2.4.1.344
-
-
?
GDP-beta-L-fucose + Galbeta(1-3)GlcNAcbeta(1-3)Galbeta(1-4)Glc-beta-O-PA8
?
show the reaction diagram
-
-
-
-
?
GDP-beta-L-fucose + lactose-N-biose I
?
show the reaction diagram
-
-
-
-
?
GDP-beta-L-fucose + lactosylceramide
?
show the reaction diagram
-
-
-
-
?
GDP-beta-L-fucose + phenyl 2-acetamido-2-deoxy-3-O-beta-D-galactopyranosyl-alpha-D-galactopyranoside
GDP + phenyl L-fucosyl-alpha (1->2)-D-Gal-beta-(1->3)-alpha-D-GalNAc
show the reaction diagram
-
-
-
-
?
GDP-beta-L-fucose + phenyl beta-D-thiogalactopyranoside
GDP + phenyl L-fucosyl-alpha-(1->2)-beta-D-thiogalactopyranoside
show the reaction diagram
-
-
-
-
?
GDP-fucose + asialofetuin
GDP + asialofetuin
show the reaction diagram
-
-
-
-
?
GDP-fucose + Galbeta-O-Bn
GDP + L-Fucalpha(1-2)Galbeta-O-Bn
show the reaction diagram
-
-
-
-
?
GDP-L-fucose + 6-sulfoGalbeta(1-4)GlcNAcbeta-O-methyl
GDP + L-Fucalpha(1-2)6-sulfoGalbeta(1-4)GlcNAcbeta-O-methyl
show the reaction diagram
-
-
-
-
?
GDP-L-fucose + agalacto-asialofetuin
GDP + alpha-L-fucosyl-1,2-agalacto-asialofetuin
show the reaction diagram
-
-
-
-
?
GDP-L-fucose + asialo alpha1 acid glycoprotein
GDP + alpha-L-fucosyl-1,2-asialo alpha1 acid glycoprotein
show the reaction diagram
-
-
-
-
?
GDP-L-fucose + asialofetuin
GDP + alpha-L-fucosyl-1,2-asialofetuin
show the reaction diagram
-
-
-
-
?
GDP-L-fucose + beta-D-galactose-beta-O-2-(2-carbomethoxyethylthio)ethyl
?
show the reaction diagram
-
-
-
-
?
GDP-L-fucose + beta-D-galactosyl-R
GDP + alpha-L-fucosyl-1,2-beta-D-galactosyl-R
show the reaction diagram
GDP-L-fucose + bovine IgG diantennary glycopeptide
GDP + ?
show the reaction diagram
-
-
-
-
?
GDP-L-fucose + D-fucose
GDP + alpha-L-fucosyl-1,2-D-fucose
show the reaction diagram
-
-
-
-
?
GDP-L-fucose + D-galactose
GDP + alpha-L-fucosyl-1,2-D-galactose
show the reaction diagram
GDP-L-fucose + di-N-acetylchitobiose
GDP + ?
show the reaction diagram
-
-
-
-
?
GDP-L-fucose + fetuin
GDP + alpha-L-fucosyl-1,2-fetuin
show the reaction diagram
-
-
-
-
?
GDP-L-fucose + fetuin triantennary asialoglycopeptide
GDP + ?
show the reaction diagram
-
-
-
-
?
GDP-L-fucose + Galbeta(1-3)GalNAc
GDP + L-Fucalpha(1-2)Galbeta(1-3)GalNAc
show the reaction diagram
-
-
-
-
?
GDP-L-fucose + Galbeta(1-3)GlcNAcbeta-O-allyl/acrylamide copolymer
GDP + ?
show the reaction diagram
-
-
-
-
?
GDP-L-fucose + Galbeta(1-3)GlcNAcbeta-O-benzyl
GDP + L-Fucalpha(1-2)Galbeta(1-3)GlcNAcbeta-O-benzyl
show the reaction diagram
-
-
-
-
?
GDP-L-fucose + Galbeta(1-4)Glc-beta-O-2-(2-carbomethoxyethylthio)ethyl
GDP + L-Fucalpha(1-2)Galbeta(1-4)Glc-beta-O-2-(2-carbomethoxyethylthio)ethyl
show the reaction diagram
-
-
-
-
?
GDP-L-fucose + Galbeta(1-4)GlcNAc-beta-O-2-(2-carbomethoxyethylthio)ethyl
GDP + L-Fucalpha(1-2)Galbeta(1-4)Glc-beta-O-2-(2-carbomethoxyethylthio)ethyl
show the reaction diagram
-
-
-
-
?
GDP-L-fucose + Galbeta(1-4)GlcNAcbeta-O-benzyl
GDP + L-Fucalpha(1-2)Galbeta(1-4)GlcNAcbeta-O-benzyl
show the reaction diagram
-
-
-
-
?
GDP-L-fucose + Galbeta(1-6)GalNAc
GDP + L-Fucalpha(1-2)Galbeta(1-6)GalNAc
show the reaction diagram
-
-
-
-
?
GDP-L-fucose + Galbeta-O-benzyl
GDP + L-Fucalpha(1-2)Galbeta-O-benzyl
show the reaction diagram
-
-
-
-
?
GDP-L-fucose + GM1 ganglioside
GDP + ?
show the reaction diagram
-
-
-
-
?
GDP-L-fucose + lacto-N-biose 1
GDP + L-Fucalpha(1-2)Galbeta(1-3)GlcNAc
show the reaction diagram
GDP-L-fucose + lacto-N-neotetraose
GDP + lacto-N-fucopentaose IV
show the reaction diagram
-
-
-
-
?
GDP-L-fucose + lacto-N-tetraose
GDP + lacto-N-fucopentaose I
show the reaction diagram
-
-
-
-
?
GDP-L-fucose + lactose
GDP + fucosyllactose
show the reaction diagram
GDP-L-fucose + N-acetylallolactosamine
GDP + ?
show the reaction diagram
-
-
-
-
?
GDP-L-fucose + N-acetyllactosamine
GDP + alpha-L-fucosyl-1,2-N-acetyllactosamine
show the reaction diagram
-
-
-
-
?
GDP-L-fucose + p-nitrophenyl beta-D-fucoside
GDP + alpha-L-fucosyl-1,2-nitrophenyl beta-D-fucoside
show the reaction diagram
-
-
-
-
?
GDP-L-fucose + phenyl-beta-D-galactoside
?
show the reaction diagram
-
-
-
-
?
GDP-L-fucose + phenyl-beta-D-galactoside
GDP + phenyl 2-O-(alpha-L-fucopyranosyl)-beta-D-galactopyranoside
show the reaction diagram
additional information
?
-
NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
GDP-beta-L-fucose + beta-D-galactosyl-(1->3)-N-acetyl-beta-D-glucosaminyl-(1->3)-beta-D-galactosyl-(1->4)-beta-D-glucosyl-(1<->1)-ceramide
GDP + alpha-L-fucosyl-(1->2)-beta-D-galactosyl-(1->3)-N-acetyl-beta-D-glucosaminyl-(1->3)-beta-D-galactosyl-(1->4)-beta-D-glucosyl-(1<->1)-ceramide
show the reaction diagram
-
-
-
?
GDP-L-fucose + beta-D-galactosyl-R
GDP + alpha-L-fucosyl-1,2-beta-D-galactosyl-R
show the reaction diagram
additional information
?
-
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
additional information
-
divalent cation is absolutely required, no activation with Zn2+
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
2-mercaptoethanol
-
1 mM: 50% loss of activity, 10 mM: 90% inhibition
3-sulfoGalbeta(1,4)GlcNAcbeta(1,6)Galbeta(1,3)GalNAcalpha-O-benzyl
-
-
beta-D-galactosyl-(1->4)-N-acetyl-D-glucosaminyl-(1->3)-beta-D-galactosyl-(1->4)-D-glucose
-
i.e. lacto-N-neotetraose, competitive with respect to lacto-N-biose I
Galbeta(1,3)GalNAcalpha-O-allyl
-
-
Galbeta(1,4)GlcNAcbeta(1,6)3-sulfoGalbeta(1,3)-GalNAcalpha-O-benzyl
-
-
Galbeta(1,4)GlcNAcbeta(1,6)Galbeta(1,3)-GalNAcalpha-O-benzyl
-
-
GDP
-
competitive
GTP
-
above 0.02 mM
PCMB
-
-
Zn2+
-
inhibition of plasma enzyme
ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
GTP
-
0.002 mM, stimulation to 160% of the control reaction
Triton X-100
-
required
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.45
6-sulfoGalbeta(1-4)GlcNAcbeta-O-methyl
-
-
2.9 - 24
beta-D-galactose
3.2 - 8.5
beta-D-galactose-beta-O-2-(2-carbomethoxyethylthio)ethyl
0.95 - 4.5
beta-D-galactosyl-(1->3)-N-acetyl-D-glucosamine
1
bovine IgG diantennary glycopeptide
-
-
-
33
di-N-acetylchitobiose
-
-
0.5
fetuin triantennary asialoglycopeptide
-
-
-
1.4 - 2.9
Galbeta(1-3)GalNAc
1.6
Galbeta(1-3)GlcNAcbeta(1-3)Galbeta(1-4)Glc-beta-O-PA8
-
H-gene encoded enzyme
0.31
Galbeta(1-3)GlcNAcbeta-O-allyl/acrylamide copolymer
-
-
-
0.83
Galbeta(1-3)GlcNAcbeta-O-benzyl
-
-
2.9 - 3.1
Galbeta(1-4)Glc-beta-O-2-(2-carbomethoxyethylthio)ethyl
2.5
Galbeta(1-4)GlcNAc-beta-O-2-(2-carbomethoxyethylthio)ethyl
-
H-endoced and Se-encoded enzyme
1.67
Galbeta(1-4)GlcNAcbeta-O-benzyl
-
-
0.82
Galbeta-O-benzyl
-
-
4.2
Galbeta1-6GalNAc
-
H-gene encoded enzyme
0.095
GDP-beta-L-fucose
-
pH 7.2, temperature not specified in the publication
0.05 - 0.178
GDP-fucose
0.006 - 1.54
GDP-L-fucose
0.082
GM1
-
recombinant enzyme
1.4 - 3.5
lacto-N-biose I
3.4 - 5.5
lacto-N-neotetraose
1.4 - 2.2
lacto-N-tetraose
2.1 - 8.9
lactose
4.9
lactose-N-biose I
-
enzyme from plasma
0.068
lactosylceramide
-
recombinant enzyme
1.1 - 5.7
N-acetyl-beta-lactosamine
8.2 - 8.79
phenyl beta-D-galactoside
3 - 125
phenyl-beta-D-galactoside
2.7 - 8.4
phenyl-beta-O-galactoside
additional information
additional information
-
KM-value for asialofetuin: 3.33 mg/ml
-
TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.007
GDP-L-fucose
-
wild type isoform FUT1, in 50 mM Tris-HCl (pH 7.2), at 37°C
0.011
phenyl-beta-D-galactoside
kcat/KM VALUE [1/mMs-1]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.0183
GDP-L-fucose
-
wild type isoform FUT1, in 50 mM Tris-HCl (pH 7.2), at 37°C
0.000088 - 0.000132
phenyl-beta-D-galactoside
Ki VALUE [mM]
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
5.6
Galbeta(1,3)GalNAcalpha-O-allyl
-
reaction with 6-sulfoGalbeta(1,3)GlcNAcbeta-O-allyl
5.9
Galbeta(1,4)GlcNAcbeta(1,6)Galbeta(1,3)-GalNAcalpha-O-benzyl
-
reaction with 6-sulfoGalbeta(1,4)GlcNAcbeta-O-methyl
0.05
GDP
-
-
0.0125
NEM
-
-
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
additional information
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
6 - 8
-
enzyme from ovarian tumor
6.5 - 7
-
-
6.8 - 8
-
-
7
-
enzyme from plasma and gastric mucosa
7.2
-
enzyme from bone marrow
7.2 - 7.6
-
recombinant enzyme
pH RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
6 - 7.6
-
pH 6.0: 15% of maximal activity, pH 6.4: 59.2% of maximal activity, pH 6.8: 84.8% of maximal activity, pH 7.2-7.6: maximal activity, recombinant enzyme
6.5 - 7.8
-
pH 6.5: about 70% of maximal activity, pH 7.8: about 65% of maximal activity, enzyme from bone marrow
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
25
-
at pH 6.0
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
-
stable transfeceted with FUT1 cDNA in a pcDNA3 vector
Manually annotated by BRENDA team
-
gut colonization by symbiotic bacteria stimulates ERK and JNK signal transduction pathways, resulting in activation of transcription factors ATF2 and c-Jun, respectively. This increases transcription of fut2 mRNA and expression of alpha1,2/3-fucosyltransferase activity, resulting in a highly fucosylated intestinal mucosa characteristic of the adult mammalian gut. Blocking the ERK and JNK signaling cascade inhibits the ability of colonization to induce elevated fut2 mRNA and fucosyltransferase activity in the mature colon
Manually annotated by BRENDA team
-
down regulation of alpha1,2-fucosyltransferase activity is associated with human gastric tumorigenesis
Manually annotated by BRENDA team
-
stable transfeceted with FUT1 cDNA in a pcDNA3 vector
Manually annotated by BRENDA team
-
an enhanced expression of CD173/CD174 and transcription of FUT1 is observed after treatment of human bone marrow endothelial cells (HBMEC) with tumor necrosis factor-alpha (TNF-alpha) or conditioned supernatants of the leukemia cell line KG1a
Manually annotated by BRENDA team
-
undifferentiated leukemic cells, K562, that have erythroid characteristics
Manually annotated by BRENDA team
-
gastric mucosa
Manually annotated by BRENDA team
-
prostatic epithelial cells derived from patients with benign prostatic hyperplasia and from patients with prostatic cancer
Manually annotated by BRENDA team
-
only when the glands are from individuals who are secretors of the ABH blood-group substances
Manually annotated by BRENDA team
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
malfunction
blocking enzyme expression in arthritis synovial fibroblasts reduces endothelial cell tube formation
physiological function
malfunction
the spontaneous G428A nonsense mutation in FUT2 provides strong but not absolute protection against symptomatic GII.4 Norovirus infection
physiological function
UNIPROT
ENTRY NAME
ORGANISM
NO. OF AA
NO. OF TRANSM. HELICES
MOLECULAR WEIGHT[Da]
SOURCE
SEQUENCE
LOCALIZATION PREDICTION?
FUT1_HUMAN
365
1
41251
Swiss-Prot
Secretory Pathway (Reliability: 1)
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
148000
-
gel filtration
150000
-
gel filtration
158000
-
gel filtration
320000
-
gel filtration
50000
-
x * 50000, enzyme from serum, SDS-PAGE under reducing conditions
65000
-
x * 65000, enzyme from gastric mucosa, SDS-PAGE
SUBUNIT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
multimer
-
x * 65000, SDS-PAGE
POSTTRANSLATIONAL MODIFICATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
glycoprotein
-
-
PROTEIN VARIANTS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
C35T
-
the mutation is a polymorphism in the Chinese population and does not affect its mRNA transcription, but can slightly decrease the activity of human alpha-(1,2)-fucosyltransferase in vitro
F280S
-
missense polymorphism, almost inactive enzyme, responsible for some non-secretor cases
G247S
-
missense polymorphism, almost inactive enzyme, responsible for some non-secretor case
I129F
most frequent nonsense mutation of FUT2 in populations of Asian ancestry
L101F
-
spontaneous missense mutation frequent in Asian populations
S12F
-
the mutation does not significantly decrease the activity of alpha-(1,2)-fucosyltransferase and affect its kinetic property
W143X
Nonsense mutation of FUT2 with a allele frequency of 0.46 in populations of European ancestry. Individuals homozygous for the FUT2 nonsecretor genotype appear to be resistant to infection with Norovirus, suggesting that individuals homozygous for nonsecretor status may be unable to mediate host-microbe interactions.
additional information
GENERAL STABILITY
ORGANISM
UNIPROT
LITERATURE
freezing and thawing has no effect
-
STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
-20°C, stable for at least 3 months
-
PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
enzyme from gastric mucosa
-
enzyme from plasma
-
enzyme from serum
-
partial
-
purification from plasma
-
CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
expressed in Sus scrofa
transfection of the mouse Fut1 and Fut2, and human FUT1 genes into human ovarian carcinoma-derived RMG-1 cells results in 20–30fold increases in cellular alpha1,2-fucosyltransferase activity, and in alteration of the glycolipid composition, including not only fucosylated products, but also precursor glycolipids
enzyme is stably transfected into Chinese hamster ovary cells
-
expressed in COS-7 cells
-
expression in NIH 3T3 cells
-
porcine endothelial cell line transfected with human alpha(1,2)-fucosyltransferase cDNA
-
subcloned in CHO-K1 cells
-
the transcription start site of FUT1 changes during differentiation of the erythroid lineage. The tissue-specific and stage-specific expression of the FUT1 are regulated by three distinct promoters. The 5'-flanking region of exon 2 consists of repetitive sequences. The start site of exon 2 is within the long terminal repeat. The repetitive sequences may play a role in the expression of the FUT1
-
EXPRESSION
ORGANISM
UNIPROT
LITERATURE
enzyme activity is increased in arthritis synovial tissue
the enzyme expression is increased in ovarian cancer. Transient overexpression of c-Jun promotes the enzyme expression 3.86 -11.4fold
APPLICATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
medicine
co-expression of the enzyme with 1,3-galactosyltransferase leads to a considerable reduction of the alpha-Gal antigen level on the surface of cells and a decrease of xenotransplant immunogenicity
analysis
-
determination of GDP-L-fucose:galactoside 2'-fucosyltransferase in human serum employing o-nitrophenyl-beta-D-galactopyranoside, phenyl-2-acetamido-2-deoxy-3-O-beta-D-galactopyranosyl-alpha-D-galactopyranoside, or phenyl beta-D-thiogalactopyranoside as acceptors. The method can simultaneously monitor possible competing reactions which may interfere with determination of alpha-2-L-fucosyltransferase activity
medicine
REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Scudder, P.R.; Chantler, E.N.
Glycosyltransferases of the human cervical epithelium. I. Characterization of a beta-galactoside alpha-2-L-fucosyltransferase and the identification of a beta-N-acetylglucosaminide alpha-3-L-fucosyltransferase
Biochim. Biophys. Acta
660
128-135
1981
Homo sapiens
Manually annotated by BRENDA team
Kyprianou, P.; Betteridge, A.; Donald, A.S.R.; Watkins, W.M.
Purification of the blood group H gene associated alpha-2-L-fucosyltransferase from human plasma
Glycoconjugate J.
7
573-588
1990
Homo sapiens
Manually annotated by BRENDA team
Chester, M.A.; Yates, A.D.; Watkins, W.M.
Phenyl beta-D-galactosylpyranoside as an acceptor substrate for the blood-group H gene-associated guanosine diphosphate L-fucose:beta-D-galactosyl alpha-2-L-fucosyltransferase
Eur. J. Biochem.
69
583-592
1976
Homo sapiens
-
Manually annotated by BRENDA team
Sarnesto, A.; Koehlin, T.; Thurin, J.; Blaszczyk-Thurin, M.
Purification of Hgene-encoded beta-galactoside alpha 1->2 fucosyltransferase from human serum
J. Biol. Chem.
265
15067-15075
1990
Homo sapiens
Manually annotated by BRENDA team
Sarnesto, A.; Koehlin, T.; Hindsgaul, O.; Thurin, J.; Blaszczyk-Thurin, M.
Purification of the secretor-type beta-galactoside alpha 1->2-fucosyltransferase from human serum
J. Biol. Chem.
267
2737-2744
1992
Homo sapiens
Manually annotated by BRENDA team
Pacuszka, T.; Koscielak, J.
alpha 1->2 Fucosyltransferase of human bone marrow
FEBS Lett.
41
348-351
1974
Homo sapiens
Manually annotated by BRENDA team
Chou, T.H.; Murphy, C.; Kessel, D.
Selective inhibition of a plasma fucosyltransferase by N-ethylmaleimide
Biochem. Biophys. Res. Commun.
74
1001-1006
1977
Homo sapiens
Manually annotated by BRENDA team
Prieto, P.A.; Larsen, R.D.; Cho, M.; Rivera, H.N.; Shilatifard, A.; Lowe, J.B.; Cummings, R.D.; Smith, D.F.
Expression of human H-type alpha1,2-fucosyltransferase encoding for blood group H(O) antigen in Chinese hamster ovary cells. Evidence for preferential fucosylation and truncation of polylactosamine sequences
J. Biol. Chem.
272
2089-2097
1997
Homo sapiens
Manually annotated by BRENDA team
Domino, S.E.; Hiraiwa, N.; Lowe, J.B.
Molecular cloning, chromosomal assignment and tissue-specific expression of a murine alpha-(1,2)fucosyltransferase expressed in thymic and epididymal epithelial cells
Biochem. J.
327
105-115
1997
Homo sapiens, Mus musculus
-
Manually annotated by BRENDA team
Masutani, H.; Kimura, H.
Purification and characterization of secretory-type GDP-L-fucose:beta-D-galactoside 2-alpha-L-fucosyltransferase from human gastric mucosa
J. Biochem.
118
541-545
1995
Homo sapiens
Manually annotated by BRENDA team
Marker, P.C.; Stephan, J.P.; Lee, J.; Bald, L.; Mather, J.P.; Cunha, G.R.
Fucosyltransferase1 and H-type complex carbohydrates modulate epithelial cell proliferation during prostatic branching morphogenesis
Dev. Biol.
233
95-108
2001
Homo sapiens, Rattus norvegicus
Manually annotated by BRENDA team
Chandrasekaran, E.V.; Chawda, R.; Locke, R.D.; Piskorz, C.F.; Matta, K.L.
Biosynthesis of the carbohydrate antigenic determinants, Globo H, blood group H, and Lewis b: a role for prostate cancer cell alpha1,2-L-fucosyltransferase
Glycobiology
12
153-162
2002
Homo sapiens
Manually annotated by BRENDA team
Koda, Y.; Soejima, M.; Kimura, H.
Changing transcription start sites in H-type alpha(1,2)fucosyltransferase gene (FUT1) during differentiation of the human erythroid lineage
Eur. J. Biochem.
256
379-387
1998
Homo sapiens
Manually annotated by BRENDA team
Chandrasekaran, E.V.; Jain, R.K.; Larsen, R.D.; Wlasichuk, K.; Matta, K.L.
Characterization of the specificities of human blood group H gene-specified alpha1,2-L-fucosyltransferase toward sulfated/sialylated/fucosylated acceptors: evidence for an inverse relationship between alpha1,2-L-fucosylation of Gal and alpha1,6-L-fucosylation of asparagine-linked GlcNAc
Biochemistry
35
8914-8924
1996
Homo sapiens
Manually annotated by BRENDA team
Yan, J.L.; Yu, L.Y.; Guo, L.H.
Human alpha galactosidase and alpha 1,2 fucosyltransferase concordantly inhibit xenoreactivity of NIH 3T3 cells with human serum
Acta Pharmacol. Sin.
24
878-884
2003
Homo sapiens
Manually annotated by BRENDA team
Mathieu, S.; Prorok, M.; Benoliel, A.M.; Uch, R.; Langlet, C.; Bongrand, P.; Gerolami, R.; El-Battari, A.
Transgene expression of alpha(1,2)-fucosyltransferase-I (FUT1) in tumor cells selectively inhibits sialyl-lewis x expression and binding to E-selectin without affecting synthesis of sialyl-Lewis a or binding to P-selectin
Am. J. Pathol.
164
371-383
2004
Homo sapiens
Manually annotated by BRENDA team
Serpa, J.; Mendes, N.; Reis, C.A.; Santos Silva, L.F.; Almeida, R.; Le Pendu, J.; David, L.
Two new FUT2 (fucosyltransferase 2 gene) missense polymorphisms, 739G-->A and 839T-->C, are partly responsible for non-secretor status in a Caucasian population from Northern Portugal
Biochem. J.
383
469-474
2004
Homo sapiens
Manually annotated by BRENDA team
Iwamori, M.; Tanaka, K.; Kubushiro, K.; Lin, B.; Kiguchi, K.; Ishiwata, I.; Tsukazaki, K.; Nozawa, S.
Alterations in the glycolipid composition and cellular properties of ovarian carcinoma-derived RMG-1 cells on transfection of the alpha1,2-fucosyltransferase gene
Cancer Sci.
96
26-30
2005
Homo sapiens (P19526), Homo sapiens, Mus musculus (P97327), Mus musculus (Q9JL27), Mus musculus
Manually annotated by BRENDA team
Sepp, A.; Skacel, P.; Lindstedt, T.; Lechler, R.I.
Expression of alpha(1,3)galactose and other type 2 oligosaccharide structures in a porcine endothelial cell line transfected with human alpha(1,2)-fucosyltransferase cDNA
J. Biol. Chem.
272
23104-23110
1997
Homo sapiens
Manually annotated by BRENDA team
Kindberg, E.; Hejdeman, B.; Bratt, G.; Wahren, B.; Lindblom, B.; Hinkula, J.; Svensson, L.
A nonsense mutation (428G-->A) in the fucosyltransferase FUT2 gene affects the progression of HIV-1 infection
AIDS
20
685-689
2006
Homo sapiens
Manually annotated by BRENDA team
Park, K.U.; Song, J.; Han, K.S.; Kim, J.Q.
The fusion allele of the FUT2 (secretor type alpha(1,2)-fucosyltransferase) gene at a high frequency and a new se385 allele in a Korean population
Ann. Hematol.
84
656-660
2005
Homo sapiens
Manually annotated by BRENDA team
Holgersson, J.; Loefling, J.
Glycosyltransferases involved in type 1 chain and Lewis antigen biosynthesis exhibit glycan and core chain specificity
Glycobiology
16
584-593
2006
Homo sapiens
Manually annotated by BRENDA team
Chandrasekaran, E.V.; Xue, J.; Piskorz, C.; Locke, R.D.; Toth, K.; Slocum, H.K.; Matta, K.L.
Potential tumor markers for human gastric cancer: an elevation of glycan:sulfotransferases and a concomitant loss of alpha1,2-fucosyltransferase activities
J. Cancer Res. Clin. Oncol.
133
599-611
2007
Homo sapiens
Manually annotated by BRENDA team
Thorven, M.; Grahn, A.; Hedlund, K.O.; Johansson, H.; Wahlfrid, C.; Larson, G.; Svensson, L.
A homozygous nonsense mutation (428G-->A) in the human secretor (FUT2) gene provides resistance to symptomatic norovirus (GGII) infections
J. Virol.
79
15351-15355
2005
Homo sapiens
Manually annotated by BRENDA team
Nilsson, C.; Skoglund, A.; Moran, A.P.; Annuk, H.; Engstrand, L.; Normark, S.
An enzymatic ruler modulates Lewis antigen glycosylation of Helicobacter pylori LPS during persistent infection
Proc. Natl. Acad. Sci. USA
103
2863-2868
2006
Homo sapiens
Manually annotated by BRENDA team
Meng, D.; Newburg, D.S.; Young, C.; Baker, A.; Tonkonogy, S.L.; Sartor, R.B.; Walker, W.A.; Nanthakumar, N.N.
Bacterial symbionts induce a FUT2-dependent fucosylated niche on colonic epithelium via ERK and JNK signaling
Am. J. Physiol. Gastrointest. Liver Physiol.
293
G780-G787
2007
Homo sapiens
Manually annotated by BRENDA team
Soejima, M.; Koda, Y.
Distinct single nucleotide polymorphism pattern at the FUT2 promoter among human populations
Ann. Hematol.
87
19-25
2008
Homo sapiens
Manually annotated by BRENDA team
Mathieu, S.; Gerolami, R.; Luis, J.; Carmona, S.; Kol, O.; Crescence, L.; Garcia, S.; Borentain, P.; El-Battari, A.
Introducing alpha(1,2)-linked fucose into hepatocarcinoma cells inhibits vasculogenesis and tumor growth
Int. J. Cancer
121
1680-1689
2007
Homo sapiens
Manually annotated by BRENDA team
Moehler, T.M.; Sauer, S.; Witzel, M.; Andrulis, M.; Garcia-Vallejo, J.J.; Grobholz, R.; Willhauck-Fleckenstein, M.; Greiner, A.; Goldschmidt, H.; Schwartz-Albiez, R.
Involvement of alpha 1-2-fucosyltransferase I (FUT1) and surface-expressed Lewis(y) (CD174) in first endothelial cell-cell contacts during angiogenesis
J. Cell. Physiol.
215
27-36
2008
Homo sapiens
Manually annotated by BRENDA team
Yip, S.P.; Lai, S.K.; Wong, M.L.
Systematic sequence analysis of the human fucosyltransferase 2 (FUT2) gene identifies novel sequence variations and alleles
Transfusion
47
1369-1380
2007
Homo sapiens (Q10981), Homo sapiens
Manually annotated by BRENDA team
Silva, L.M.; Carvalho, A.S.; Guillon, P.; Seixas, S.; Azevedo, M.; Almeida, R.; Ruvoen-Clouet, N.; Reis, C.A.; Le Pendu, J.; Rocha, J.; David, L.
Infection-associated FUT2 (Fucosyltransferase 2) genetic variation and impact on functionality assessed by in vivo studies
Glycoconj. J.
27
61-68
2010
Homo sapiens
Manually annotated by BRENDA team
Abrantes, J.; Posada, D.; Guillon, P.; Esteves, P.J.; Le Pendu, J.
Widespread gene conversion of alpha-2-fucosyltransferase genes in mammals
J. Mol. Evol.
69
22-31
2009
Bos taurus (A4IFH1), Bos taurus (Q28113), Bos taurus (Q9TTY3), Chlorocebus sabaeus, Chlorocebus sabaeus (O77711), Chlorocebus sabaeus (O77712), Gorilla gorilla (O77486), Gorilla gorilla (Q9TUD4), Homo sapiens (P19526), Homo sapiens (Q10981), Homo sapiens (Q5XLR8), Homo sapiens, Hylobates agilis (Q6LA46), Hylobates lar (Q9TTC7), Macaca fascicularis (Q9N266), Monodelphis domestica, Mus musculus (O09160), Mus musculus (P97353), Oryctolagus cuniculus (Q10979), Oryctolagus cuniculus (Q10983), Oryctolagus cuniculus (Q29505), Oryctolagus cuniculus, Pan troglodytes (O77485), Pan troglodytes (Q9TUD6), Pongo pygmaeus (O77487), Pongo pygmaeus (Q6LA45), Rattus norvegicus (Q10980), Rattus norvegicus (Q10984), Rattus norvegicus (Q9WUE6), Sus scrofa (Q10982), Sus scrofa (Q29043), Xenopus tropicalis
Manually annotated by BRENDA team
Hazra, A.; Kraft, P.; Selhub, J.; Giovannucci, E.L.; Thomas, G.; Hoover, R.N.; Chanock, S.J.; Hunter, D.J.
Common variants of FUT2 are associated with plasma vitamin B12 levels
Nat. Genet.
40
1160-1162
2008
Homo sapiens (Q10981)
Manually annotated by BRENDA team
Carlsson, B.; Kindberg, E.; Buesa, J.; Rydell, G.E.; Lidon, M.F.; Montava, R.; Abu Mallouh, R.; Grahn, A.; Rodriguez-Diaz, J.; Bellido, J.; Arnedo, A.; Larson, G.; Svensson, L.
The G428A nonsense mutation in FUT2 provides strong but not absolute protection against symptomatic GII.4 Norovirus infection
PLoS ONE
4
e5593
2009
Homo sapiens (Q10981)
Manually annotated by BRENDA team
Tao, S.; He, Y.; Ying, Y.; Hong, X.; Xu, X.; Zhu, F.; Lv, H.; Yan, L.
C35T mutation could slightly decrease the activity of human alpha-(1,2)-fucosyltransferase
Transfus. Clin. Biol.
19
5-10
2012
Homo sapiens
Manually annotated by BRENDA team
Zeyland, J.; Wozniak, A.; Gawronska, B.; Juzwa, W.; Jura, J.; Nowak, A.; S?omski, R.; Smorag, Z.; Szalata, M.; Mazurek, U.; Lipinski, D.
Double transgenic pigs with combined expression of human alpha1,2-fucosyltransferase and alpha-galactosidase designed to avoid hyperacute xenograft rejection
Arch. Immunol. Ther. Exp.
62
411-422
2014
Homo sapiens (P19526)
Manually annotated by BRENDA team
Isozaki, T.; Ruth, J.H.; Amin, M.A.; Campbell, P.L.; Tsou, P.S.; Ha, C.M.; Haines, G.K.; Edhayan, G.; Koch, A.E.
Fucosyltransferase 1 mediates angiogenesis, cell adhesion and rheumatoid arthritis synovial tissue fibroblast proliferation
Arthritis Res. Ther.
16
R28
2014
Homo sapiens (P19526)
Manually annotated by BRENDA team
Gao, N.; Liu, J.; Liu, D.; Hao, Y.; Yan, L.; Ma, Y.; Zhuang, H.; Hu, Z.; Gao, J.; Yang, Z.; Shi, H.; Lin, B.
c-Jun transcriptionally regulates alpha 1, 2-fucosyltransferase 1 (FUT1) in ovarian cancer
Biochimie
107 Pt B
286-292
2014
Homo sapiens (P19526)
Manually annotated by BRENDA team
Che, Y.; Ren, X.; Xu, L.; Ding, X.; Zhang, X.; Sun, X.
Critical involvement of the alpha(1,2)-fucosyltransferase in multidrug resistance of human chronic myeloid leukemia
Oncol. Rep.
35
3025-3033
2016
Homo sapiens (P19526)
Manually annotated by BRENDA team
Dicioccio, R.A.; Barlow, J.J.; Matta, K.L.
Modified procedure for detection of GDP-L-fucose:galactoside 2-fucosyltransferase
Clin. Chim. Acta
108
41-48
1980
Homo sapiens
Manually annotated by BRENDA team
Kyprianou, P.; Betteridge, A.; Donald, A.S.; Watkins, W.M.
Purification of the blood group H gene associated alpha-2-L-fucosyltransferase from human plasma
Glycoconj. J.
7
573-588
1990
Homo sapiens
Manually annotated by BRENDA team