Information on EC 2.4.1.56 - lipopolysaccharide N-acetylglucosaminyltransferase

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The expected taxonomic range for this enzyme is: Proteobacteria

EC NUMBER
COMMENTARY hide
2.4.1.56
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RECOMMENDED NAME
GeneOntology No.
lipopolysaccharide N-acetylglucosaminyltransferase
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
UDP-N-acetyl-alpha-D-glucosamine + lipopolysaccharide = UDP + N-acetyl-alpha-D-glucosaminyllipopolysaccharide
show the reaction diagram
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-
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REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
hexosyl group transfer
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-
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PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
Lipopolysaccharide biosynthesis
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Metabolic pathways
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SYSTEMATIC NAME
IUBMB Comments
UDP-N-acetyl-D-glucosamine:lipopolysaccharide N-acetyl-D-glucosaminyltransferase
Transfers N-acetylglucosaminyl residues to a D-galactose residue in the partially completed lipopolysaccharide core [cf. EC 2.4.1.44 (lipopolysaccharide 3-alpha-galactosyltransferase), EC 2.4.1.58 (lipopolysaccharide glucosyltransferase I) and EC 2.4.1.73 (lipopolysaccharide glucosyltransferase II)].
CAS REGISTRY NUMBER
COMMENTARY hide
37277-64-8
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ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
strains 35000HP and 35000HP-RSM212
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Manually annotated by BRENDA team
strain H44/76, immunotype L3
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-
Manually annotated by BRENDA team
UDPgalactose-4-epimerase mutant
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Manually annotated by BRENDA team
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
UDP-N-acetyl-D-galactosamine + lipooligosacccharide
UDP + N-acetyl-D-galactosaminyllipooligosaccharide
show the reaction diagram
UDP-N-acetyl-D-glucosamine + beta-D-Galp-(1-4)-beta-D-Glcp-O-(CH2)6-NH-dansyl
?
show the reaction diagram
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-
-
-
?
UDP-N-acetyl-D-glucosamine + Galbeta1-3GalNAcbeta-O-p-nitrophenol
UDP + GlcNAcbeta-1-3Galbeta1-3GalNAcbeta-O-p-nitrophenol
show the reaction diagram
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low affinity
-
-
?
UDP-N-acetyl-D-glucosamine + Galbeta1-4Glc lipooligosaccharide
UDP + GlcNAcbeta1-3Galbeta1-4Glc lipooligosaccharide
show the reaction diagram
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low affinity
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-
?
UDP-N-acetyl-D-glucosamine + Galbeta1-4Glcbeta-O-p-nitrophenol
UDP + GlcNAcbeta1-3Galbeta1-4Glcbeta-O-p-nitrophenol
show the reaction diagram
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-
-
-
?
UDP-N-acetyl-D-glucosamine + Galbeta1-4GlcNAcbeta-O-p-nitrophenol
UDP + GlcNAcbeta1-3-Galbeta1-4GlcNAcbeta-O-p-nitrophenol
show the reaction diagram
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-
-
-
?
UDP-N-acetyl-D-glucosamine + lactose
UDP + N-acetyl-D-glucosaminyllactose
show the reaction diagram
UDP-N-acetyl-D-glucosamine + lipooligosacccharide
UDP + N-acetyl-D-glucosaminyllipooligosaccharide
show the reaction diagram
UDP-N-acetyl-D-glucosamine + lipopolysaccharide
?
show the reaction diagram
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-
-
-
?
UDP-N-acetyl-D-glucosamine + lipopolysaccharide
UDP + N-acetyl-D-glucosaminyllipopolysaccharide
show the reaction diagram
UDP-N-acetyl-D-glucosamine + N-acetyllactosamine
UDP + N-acetyl-D-glucosaminyl-N-acetyllactosamine
show the reaction diagram
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enzyme also acts efficiently on monomeric and dimeric N-acetyllactosamine
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?
additional information
?
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NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
UDP-N-acetyl-D-glucosamine + lipooligosacccharide
UDP + N-acetyl-D-glucosaminyllipooligosaccharide
show the reaction diagram
UDP-N-acetyl-D-glucosamine + lipopolysaccharide
UDP + N-acetyl-D-glucosaminyllipopolysaccharide
show the reaction diagram
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involved in synthesis of cell wall lipopolysaccharides, transfers N-acetylglucosaminyl residues to a D-glucose residue in the partially completed lipopolysaccharide core, the results are consistent with the postulated attachment of N-acetylglucosamine to glucose, but do not exclude linkage to another residue
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?
additional information
?
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METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Fe2+
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activates, less effective than Mn2+
Mg2+
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activates, less effective than Mn2+
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
2-acetamido-2-deoxy-alpha-D-glucopyranosyl 1-diphosphate
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UDP
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1.5 mM: 50% inhibition
additional information
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not inhibited by UMP
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KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
27
Galbeta1-3GalNAcbeta-O-p-nitrophenol lipooligosaccharide
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UDP-N-acetyl-D-glucosamine as donor
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28 - 36
Galbeta1-4Glc lipooligosaccharide
4.3 - 4.5
Galbeta1-4Glcbeta-O-p-nitrophenol lipooligosaccharide
6.1 - 7.2
Galbeta1-4GlcNAcbeta-O-p-nitrophenol lipooligosaccharide
0.016 - 0.052
lipopolysaccharide
2.4
UDP-N-acetyl-D-galactosamine
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0.04 - 0.22
UDP-N-acetyl-D-glucosamine
kcat/KM VALUE [1/mMs-1]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
2.5 - 25
lipopolysaccharide
1 - 10
UDP-N-acetyl-D-glucosamine
IC50 VALUE [mM]
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
7
2-acetamido-2-deoxy-alpha-D-glucopyranosyl 1-diphosphate
Neisseria meningitidis
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SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
0.08 - 0.09
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pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
pH RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
additional information
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broad pH-range within enzyme is active
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
additional information
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cell-wall membrane fraction
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Manually annotated by BRENDA team
STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
-20°C, 50% v/v glycerol, 5mg/ml bovine serum albumin, at least 5 months, stable
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Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
His-Tag affinity chromatography
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partial, recombinant enzyme expressed in Escherichia coli
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Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
cloning and overexpression of lgtA gene encoding enzyme in Escherichia coli BL21(DE3)/pLys, polypeptide of 333 amino acids
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expressed in Escherichia coli
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lgtA gene encoding N-acetylglucosamine glycosyltransferase is cloned and expressed in Escherichia coli DH5alphapcnB, predicted amino acid sequence
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lgtA lipooligosaccharide N-acetylglucosamine glycosyltransferase gene, amino acid sequence
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overexpression in Escherichia coli BL21
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rfaK gene encodes the alpha-1,2-N-acetylglucosamine transferase, 1065-bp open reading frame, rfaK gene is cotranscribed with lgtF as polycistronic message from a promoter upstream of lgtF: ice operon, lgtA gene encodes beta-1,3-N-acetylglucosamine transferase
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ENGINEERING
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
additional information
APPLICATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
synthesis
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