Information on EC 2.4.1.48 - heteroglycan alpha-mannosyltransferase

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The expected taxonomic range for this enzyme is: Dikarya

EC NUMBER
COMMENTARY
2.4.1.48
-
RECOMMENDED NAME
GeneOntology No.
heteroglycan alpha-mannosyltransferase
REACTION
REACTION DIAGRAM
COMMENTARY
ORGANISM
UNIPROT ACCESSION NO.
LITERATURE
GDP-mannose + heteroglycan = GDP + 2(or 3)-alpha-D-mannosyl-heteroglycan
show the reaction diagram
-
-
-
-
REACTION TYPE
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
hexosyl group transfer
-
-
-
-
SYSTEMATIC NAME
IUBMB Comments
GDP-mannose:heteroglycan 2-(or 3-)-alpha-D-mannosyltransferase
The acceptor is a heteroglycan primer containing mannose, galactose and xylose. 1,2- and 1,3-mannosyl bonds are formed.
SYNONYMS
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
GDP mannose alpha-mannosyltransferase
-
-
-
-
guanosine diphosphomannose-heteroglycan alpha-mannosyltransferase
-
-
-
-
mannosyltransferase, guanosine diphosphomannose-heteroglycan alpha-
-
-
-
-
CAS REGISTRY NUMBER
COMMENTARY
37277-57-9
-
ORGANISM
COMMENTARY
LITERATURE
SEQUENCE CODE
SEQUENCE DB
SOURCE
Cryptococcus laurentii var. flavescens
NRRL Y-1401
-
-
Manually annotated by BRENDA team
Cryptococcus laurentii var. flavescens NRRL Y-1401
NRRL Y-1401
-
-
Manually annotated by BRENDA team
strain 66.24
-
-
Manually annotated by BRENDA team
Saccharomyces cerevisiae 66.24
strain 66.24
-
-
Manually annotated by BRENDA team
SUBSTRATE
PRODUCT                      
REACTION DIAGRAM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
(Substrate)
LITERATURE
(Substrate)
COMMENTARY
(Product)
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
GDP-mannose + heteroglycan
GDP + 1,2(or 1,3)-alpha-D-mannosylheteroglycan
show the reaction diagram
Cryptococcus laurentii var. flavescens, Cryptococcus laurentii var. flavescens NRRL Y-1401
-
-
-
?
GDP-mannose + heteropolysaccharide
GDP + 1,2(or 1,3)-alpha-D-mannosylheteropolysaccharide
show the reaction diagram
Cryptococcus laurentii var. flavescens, Cryptococcus laurentii var. flavescens NRRL Y-1401
-
heteropolysaccharide containing mannosyl, galactosyl, xylosyl units
-
?
GDP-mannose + mannose
GDP + O-alpha-D-mannosyl-1,2-mannose
show the reaction diagram
Saccharomyces cerevisiae, Saccharomyces cerevisiae 66.24
-
-
-
?
GDP-mannose + methyl-alpha-D-mannoside
?
show the reaction diagram
Saccharomyces cerevisiae, Saccharomyces cerevisiae 66.24
-
-
-
-
?
additional information
?
-
Cryptococcus laurentii var. flavescens, Cryptococcus laurentii var. flavescens NRRL Y-1401
-
mannose-1-phosphate and mannose are no substrates
-
-
-
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
(Substrate)
LITERATURE
(Substrate)
COMMENTARY
(Product)
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
GDP-mannose + heteroglycan
GDP + 1,2(or 1,3)-alpha-D-mannosylheteroglycan
show the reaction diagram
Cryptococcus laurentii var. flavescens, Cryptococcus laurentii var. flavescens NRRL Y-1401
-
-
-
?
METALS and IONS
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
Ca2+
Cryptococcus laurentii var. flavescens
-
20 mM, required, 15% of activity compared to Mn2+
Co2+
Cryptococcus laurentii var. flavescens
-
20 mM, required, 16% of activity compared to Mn2+
Cu2+
Cryptococcus laurentii var. flavescens
-
20 mM, required, about 1% of activity compared to Mn2+
Mg2+
Cryptococcus laurentii var. flavescens
-
20 mM, required, 35% of activity compared to Mn2+
Mn2+
Cryptococcus laurentii var. flavescens
-
20 mM, required, 100% activity
Ni2+
Cryptococcus laurentii var. flavescens
-
20 mM, required, 4% of activity compared to Mn2+
Zn2+
Cryptococcus laurentii var. flavescens
-
20 mM, required, 16% of activity compared to Mn2+
Mn2+
-
essential for reaction, cannot be replaced by Mg2+
additional information
Cryptococcus laurentii var. flavescens
-
absolute requirement for a divalent cation
KM VALUE [mM]
KM VALUE [mM] Maximum
SUBSTRATE
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
IMAGE
0.14
-
GDP-mannose
Cryptococcus laurentii var. flavescens
-
-
0.25
-
GDPmannose
-
-
SPECIFIC ACTIVITY [µmol/min/mg]
SPECIFIC ACTIVITY MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
additional information
-
-
-
pH OPTIMUM
pH MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
7.5
-
Cryptococcus laurentii var. flavescens
-
-
8
-
-
formation of mannobiose
pH RANGE
pH RANGE MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
6
10
Cryptococcus laurentii var. flavescens
-
20% of maximal activity at pH 6 and pH 10
6
8.5
-
50% of maximal activity at pH 6, 90% of maximal activity at pH 8.5
TEMPERATURE OPTIMUM
TEMPERATURE OPTIMUM MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
25
-
Cryptococcus laurentii var. flavescens
-
assay at
LOCALIZATION
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
GeneOntology No.
LITERATURE
SOURCE
Cryptococcus laurentii var. flavescens
-
cell wall associated
Manually annotated by BRENDA team
Cryptococcus laurentii var. flavescens NRRL Y-1401
-
cell wall associated
-
Manually annotated by BRENDA team
GENERAL STABILITY
ORGANISM
UNIPROT ACCESSION NO.
LITERATURE
repeated freezing and thawing reduces activity
Cryptococcus laurentii var. flavescens
-
STORAGE STABILITY
ORGANISM
UNIPROT ACCESSION NO.
LITERATURE
-20°C, stable for at least 4 weeks
Cryptococcus laurentii var. flavescens
-
Purification/COMMENTARY
ORGANISM
UNIPROT ACCESSION NO.
LITERATURE
partial
Cryptococcus laurentii var. flavescens
-