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Information on EC 2.4.1.41 - polypeptide N-acetylgalactosaminyltransferase and Organism(s) Rattus norvegicus and UniProt Accession O88422

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EC Tree
     2 Transferases
         2.4 Glycosyltransferases
             2.4.1 Hexosyltransferases
                2.4.1.41 polypeptide N-acetylgalactosaminyltransferase
IUBMB Comments
Requires both Mn2+ and Ca2+. The glycosyl residue is transferred to threonine or serine hydroxy groups on the polypeptide core of submaxillary mucin, kappa-casein, apofetuin and some other acceptors of high molecular mass.
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This record set is specific for:
Rattus norvegicus
UNIPROT: O88422
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Word Map
The taxonomic range for the selected organisms is: Rattus norvegicus
The expected taxonomic range for this enzyme is: Eukaryota, Bacteria, Archaea
Synonyms
galnt3, n-acetylgalactosaminyltransferase, galnt2, galnac-t3, galnac-t, galnt14, galnac-transferase, galnac transferase, galnac-t2, galnac-t1, more
SYNONYM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
UDP-GalNAc:polypeptide N-acetylgalactosaminyltransferase
-
acetylgalactosaminyltransferase, uridine diphosphoacetylgalactosamine-glycoprotein
-
-
-
-
GalNAc transferase
-
GalNAc-transferase
-
-
-
-
glycoprotein acetylgalactosaminyltransferase
-
-
-
-
polypeptide-N-acetylgalactosamine transferase
-
-
-
-
ppGalNAc-T
-
-
-
-
ppGalNAc-T10
-
-
ppGaNTase
-
-
-
-
ppGaNTase-T1
ppGaNTase-T3
-
-
ppGaNTase-T4
-
-
protein-UDP acetylgalactosaminyltransferase
-
-
-
-
UDP-acetylgalactosamine-glycoprotein acetylgalactosaminyltransferase
-
-
-
-
UDP-acetylgalactosamine:peptide-N-galactosaminyltransferase
-
-
-
-
UDP-GalNAc:polypeptide N-acetylgalactosaminyl transferase
-
-
-
-
UDP-GalNAc:polypeptide N-acetylgalactosaminyltransferase
UDP-GalNAc:polypeptide N-acetylgalactosaminyltransferase 9
-
-
UDP-N-acetyl-alpha-D-galactosamine:polypeptide N-acetylgalactosaminyltransferase
-
-
-
-
UDP-N-acetyl-D-galactosamine:polypeptide N-acetylgalactosaminyltransferase-T10
-
-
UDP-N-acetylgalactosamine-glycoprotein N-acetylgalactosaminyltransferase
-
-
-
-
UDP-N-acetylgalactosamine-protein N-acetylgalactosaminyltransferase
-
-
-
-
UDP-N-acetylgalactosamine:kappa-casein polypeptide N-acetylgalactosaminyltransferase
-
-
-
-
UDP-N-acetylgalactosamine:polypeptide N-acetylgalactosaminyltransferase
-
-
-
-
UDP-N-acetylgalactosamine:protein N-acetylgalactosaminyl transferase
-
-
-
-
uridine diphosphoacetylgalactosamine-glycoprotein acetylgalactosaminyltransferase
-
-
-
-
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
hexosyl group transfer
SYSTEMATIC NAME
IUBMB Comments
UDP-N-acetyl-D-galactosamine:polypeptide N-acetylgalactosaminyl-transferase
Requires both Mn2+ and Ca2+. The glycosyl residue is transferred to threonine or serine hydroxy groups on the polypeptide core of submaxillary mucin, kappa-casein, apofetuin and some other acceptors of high molecular mass.
CAS REGISTRY NUMBER
COMMENTARY hide
9075-15-4
-
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
UDP-N-acetyl-D-galactosamine + PTTDSTTPAPTTK
UDP + ?
show the reaction diagram
enzyme form ppGaNTase-T5
-
-
?
UDP-GalNAc + apomucin
UDP + GalNAc-mucin
show the reaction diagram
apomucin is a deglycosylated bovine submaxillary mucin, mutants W328Y, W328F, W328A, W316H and W316A are inactive, mutants W316Y and W316F show 40% and 20% activity, respectively, relative to wild-type
-
-
?
UDP-GalNAc + GVVPTVVPG
UDP + GVVP(GalNAc)TVVPG
show the reaction diagram
GVVPTVVPG is a synthetic peptide with threonine as acceptor site, weaker acceptor substrate for wild-type and truncated version P-T1/delta42 as PPDAATAAPL
-
-
?
UDP-GalNAc + PPDAATAAPL
UDP + PPDAA(GalNAc)TAAPL
show the reaction diagram
PPDAATAAPL is a synthetic peptide with threonine as acceptor site, efficient acceptor substrate for wild-type and truncated version P-T1/delta42
-
-
?
UDP-N-acetyl-alpha-D-galactosamine + Gly-Val-Val-Pro-Thr-Val-Val-Pro-Gly
?
show the reaction diagram
-
-
-
-
?
UDP-N-acetyl-alpha-D-galactosamine + Pro-Pro-Asp-Ala-Ala-Thr-Ala-Ala-Pro-Leu
?
show the reaction diagram
-
-
-
-
?
UDP-N-acetyl-D-galactosamine + A1 protein
UDP + N-acetyl-D-galactosaminyl-A1 protein
show the reaction diagram
-
-
-
-
?
UDP-N-acetyl-D-galactosamine + apoantifreeze glycoproteins
UDP + N-acetyl-D-galactosaminyl-apoantifreeze glycoproteins
show the reaction diagram
-
apoantifreeze glycoprotein of high molecular weight accepts GalNAc much faster than apoantifreeze glycoprotein 8
-
-
?
UDP-N-acetyl-D-galactosamine + apofetuin
UDP + N-acetyl-D-galactosaminyl-apofetuin
show the reaction diagram
-
lowest rate of transfer
-
-
?
UDP-N-acetyl-D-galactosamine + apomucin
UDP + N-acetyl-D-galactosaminyl-apomucin
show the reaction diagram
UDP-N-acetyl-D-galactosamine + GalNAc-glycosylated peptide
UDP + N-acetyl-D-galactosaminyl-GalNAc-glucosylated peptide
show the reaction diagram
UDP-N-acetyl-D-galactosamine + GTTPSPVPTTS-(O-GalNAc)T-SAP
UDP + ?
show the reaction diagram
-
-
-
-
?
UDP-N-acetyl-D-galactosamine + kappa-casein
UDP + N-acetyl-D-galactosaminyl-kappa-casein
show the reaction diagram
-
-
-
-
?
UDP-N-acetyl-D-galactosamine + polypeptide
UDP + N-acetyl-D-galactosaminyl-polypeptide
show the reaction diagram
UDP-N-acetyl-D-galactosamine + PTTDS-(O-GalNAc)T-TPAPTTK
UDP + ?
show the reaction diagram
-
38.7% of the activity with GTTPSPVPTTS-(O-GalNAc)T-TSAP
-
-
?
UDP-N-acetyl-D-galactosamine + PTTDSTTPAPTTK
UDP + ?
show the reaction diagram
additional information
?
-
NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
UDP-N-acetyl-D-galactosamine + polypeptide
UDP + N-acetyl-D-galactosaminyl-polypeptide
show the reaction diagram
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Co2+
-
less stimulation than by Mn2+
Cu2+
-
less stimulation than by Mn2+
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
EDTA
-
10 mM, complete inhibition
GalNAc
-
inhibition of GalNAc-T1 by free GalNAc: follow-up O-glycosylation of apomucin is sensitive, O-glycosylation of synthetic peptides containing a single glycosylation site is resistant
UDP
-
competitive
UMP
-
uncompetitive
UTP
-
competitive
additional information
-
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.113
PTTDSTTPAPTTK
enzyme form ppGaNTase-T5
0.8
Gly-Val-Val-Pro-Thr-Val-Val-Pro-Gly
-
GalNAc-T1
0.0209
GTTPSPVPTTS-(O-GalNAc)T-SAP
-
pH 7.0, 37°C
1.74 - 17.6
GVVPTVVPG
0.35 - 15.6
PPDAATAAPL
0.2
Pro-Pro-Asp-Ala-Ala-Thr-Ala-Ala-Pro-Leu
-
GalNAc-T1
0.1058
PTTDS-(O-GalNAc)T-TPAPTTK
-
pH 7.0, 37°C
0.042 - 1.02
PTTDSTTPAPTTK
0.0417 - 16
UDP-GalNAc
0.0141
UDP-N-acetyl-D-galactosamine
-
pH 7.0, 37°C
additional information
apomucin
-
Ki VALUE [mM]
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
68 - 175
GalNAc
0.026
UDP
-
-
0.347
UMP
-
-
0.0835
UTP
-
-
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
2.12
-
purified enzyme
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
-
SwissProt
Manually annotated by BRENDA team
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
-
dorsal root
Manually annotated by BRENDA team
ppGaNTase-T9, less amount
Manually annotated by BRENDA team
-
AH-66 ascites hepatoma cells, harvested 6-7 days after the intraperitoneal transfer of ascites fluid from donor rats, activity much higher than in rat liver
Manually annotated by BRENDA team
-
expresses GalNAc-T2
Manually annotated by BRENDA team
ppGaNTase-T9, less amount
Manually annotated by BRENDA team
ppGaNTase-T9
Manually annotated by BRENDA team
-
cerebellar
Manually annotated by BRENDA team
ppGaNTase-T9, less amount
Manually annotated by BRENDA team
ppGaNTase-T9, less amount
Manually annotated by BRENDA team
ppGaNTase-T9, less amount
Manually annotated by BRENDA team
additional information
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
UNIPROT
ENTRY NAME
ORGANISM
NO. OF AA
NO. OF TRANSM. HELICES
MOLECULAR WEIGHT[Da]
SOURCE
SEQUENCE
LOCALIZATION PREDICTION?
GALT5_RAT
930
1
105120
Swiss-Prot
Secretory Pathway (Reliability: 3)
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
54000
-
gel filtration
56000
-
1 * 56000, SDS-PAGE in presence of 2-mercaptoethanol
SUBUNIT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
monomer
-
1 * 56000, SDS-PAGE in presence of 2-mercaptoethanol
PROTEIN VARIANTS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
D444A
-
GalNAc-T1 mutant with severely impaired apomucin glycosylation, D444A/D484A/D525A triple mutant has significantly lower activity than D444A single mutant
F303L
mutant of 40% activity relative to truncated wild-type using apomucin as acceptor substrate and of increased KM for both substrates
F325L
mutant of 60% activity relative to truncated wild-type using apomucin as acceptor substrate
F468A
-
GalNAc-T1 mutant with strongly reduced activity and decreased expression
G455Q
-
GalNAc-T1 mutant with reduced reactivity towards apomucin
P-T1-delta42
truncated GalNAc-T1 wild-type, deletion of the coding sequence for the cytoplasmic tail and the transmembrane domain, cDNA for insulin signal sequence and protein A IgG-binding domain fused to the 5’-end
W316A
inactive mutant using apomucin as acceptor substrate
W316F
mutant of 20% activity relative to truncated wild-type using apomucin as acceptor substrate and of increased KMs for apomucin, UDP-GalNAc, and synthetic peptides, PPDAATAAPL and GVVPTVVPG
W316L
background level activity relative to truncated wild-type using apomucin as acceptor substrate
W316Y
mutant of 40% activity relative to truncated wild-type using apomucin as acceptor substrate and of increased KMs for apomucin, UDP-GalNAc, and synthetic peptides, PPDAATAAPL and GVVPTVVPG
W328A
inactive mutant using apomucin as acceptor substrate, low expression level
W328F
inactive mutant using apomucin as acceptor substrate
W328L
inactive mutant using apomucin as acceptor substrate
W328Y
inactive mutant using apomucin as acceptor substrate
Y302L
mutant of 80% activity relative to truncated wild-type using apomucin as acceptor substrate
Y309L
mutant of 40% activity relative to truncated wild-type using apomucin as acceptor substrate and of increased KM for both substrates
additional information
STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
-20°C, 3 months, stable
-
PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
recombinant mutant GalNAc-T1, expressed in COS7 cells
-
recovery of the secreted recombinant proteins that harbour a protein A IgG-binding domain by IgG-Sepharose
CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
deletion mutant GalNAc-T1 cDNA is cloned and expressed in COS7 cells
-
expression in COS7 cells as truncated version from pInsProA
gene encoding ppGaNTase-T9 from sublingual gland is cloned, characterized, sequenced and expressed transiently in COS7 cells, gene encodes a unique 603-amino acid protein
ppGaNTase-T6 from sublingual gland is cloned, sequenced and expressed transiently in COS7 cells as secreted form, 657-amino acid protein, amino acid sequences of ppGaNTase-T1, -T5 and -T6
APPLICATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
molecular biology
invariant residue Trp328 is essential for GalNAc-T enzymatic activity, residue Trp316 is important in the interaction with the acceptor polypeptide of GalNAc-T1
REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Sugiura, M.; Kawasaki, T.; Yamashina, I.
Purification and characterization of UDP-GalNAc:polypeptide N-acetylgalactosamine transferase from an ascites hepatoma, AH 66
J. Biol. Chem.
257
9501-9507
1982
Rattus norvegicus
Manually annotated by BRENDA team
Soerensen, T.; White, T.; Wandall, H.H.; Kristensen, A.K.; Roepstorff, P.; Clausen, H.
UDP-N-acetyl-alpha-D-galactosamine:polypeptide N-acetylgalactosaminyltransferase. Identification and separation of two distinct transferase activities
J. Biol. Chem.
270
24166-24173
1995
Homo sapiens, Ovis sp., Rattus norvegicus, Sus scrofa
Manually annotated by BRENDA team
Ten Hagen, K.G.; Bedi, G.S.; Tetaert, D.; Kingsley, P.D.; Hagen, F.K.; Balys, M.M.; Beres, T.M.; Degand, P.; Tabak, L.A.
Cloning and characterization of a ninth member of the UDP-GalNAc:polypeptide N-acetylgalactosaminyltransferase family, ppGaNTase-T9
J. Biol. Chem.
276
17395-17404
2001
Mus musculus, Rattus norvegicus (Q925R7)
Manually annotated by BRENDA team
Ten Hagen, K.G.; Tetaert, D.; Hagen, F.K.; Richet, C.; Beres, T.M.; Gagnon, J.; Balys, M.M.; VanWuyckhuyse, B.; Bedi, G.S.; Degand, P.; Tabak, L.A.
Characterization of a UDP-GalNAc:polypeptide N-acetylgalactosaminyltransferase that displays glycopeptide N-acetylgalactosaminyltransferase activity
J. Biol. Chem.
274
27867-27874
1999
Mus musculus, Rattus norvegicus (Q9R0C5)
Manually annotated by BRENDA team
Tenno, M.; Saeki, A.; Kezdy, F.J.; Elhammer, A.P.; Kurosaka, A.
The lectin domain of UDP-GalNAc:polypeptide N-acetylgalactosaminyltransferase 1 is involved in O-glycosylation of a polypeptide with multiple acceptor sites
J. Biol. Chem.
277
47088-47096
2002
Rattus norvegicus
Manually annotated by BRENDA team
Stwora-Wojczyk, M.M.; Dzierszinski, F.; Roos, D.S.; Spitalnik, S.L.; Wojczyk, B.S.
Functional characterization of a novel Toxoplasma gondii glycosyltransferase: UDP-N-acetyl-D-galactosamine:polypeptide N-acetylgalactosaminyltransferase-T3
Arch. Biochem. Biophys.
426
231-240
2004
Mus musculus, Rattus norvegicus, Toxoplasma gondii (Q6YBY0), Toxoplasma gondii
Manually annotated by BRENDA team
Nakamura, N.; Toba, S.; Hirai, M.; Morishita, S.; Mikami, T.; Konishi, M.; Itoh, N.; Kurosaka, A.
Cloning and expression of a brain-specific putative UDP-GalNAc:polypeptide N-acetylgalactosaminyltransferase gene
Biol. Pharm. Bull.
28
429-433
2005
Rattus norvegicus, Homo sapiens (Q9HCQ5), Homo sapiens
Manually annotated by BRENDA team
Hagen, F.K.; Gregoire, C.A.; Tabak, L.A.
Cloning and sequence homology of a rat UDP-GalNAc:polypeptide N-acetylgalactosaminyltransferase
Glycoconj. J.
12
901-909
1995
Rattus norvegicus (Q10473)
Manually annotated by BRENDA team
Ten hagen, K.G.; Hagen, F.K.; Balys, M.; Beres, T.M.; van Wuyckhuyse, B.; Tabak, L.A.
Cloning and expression of a novel, tissue specifically expressed member of the UDP-GalNAc:polypeptide N-acetylgalactosaminyltransferase family
J. Biol. Chem.
273
27749-27754
1998
Rattus norvegicus, Rattus norvegicus (O88422)
Manually annotated by BRENDA team
Tenno, M.; Saeki, A.; Elhammer, A.P.; Kurosaka, A.
Function of conserved aromatic residues in the Gal/GalNAc-glycosyltransferase motif of UDP-GalNAc:polypeptide N-acetylgalactosaminyltransferase 1
FEBS J.
274
6037-6045
2007
Rattus norvegicus (Q10473)
Manually annotated by BRENDA team