Information on EC 2.4.1.40 - glycoprotein-fucosylgalactoside alpha-N-acetylgalactosaminyltransferase

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The expected taxonomic range for this enzyme is: Bacteria, Eukaryota

EC NUMBER
COMMENTARY
2.4.1.40
-
RECOMMENDED NAME
GeneOntology No.
glycoprotein-fucosylgalactoside alpha-N-acetylgalactosaminyltransferase
REACTION
REACTION DIAGRAM
COMMENTARY
ORGANISM
UNIPROT ACCESSION NO.
LITERATURE
UDP-N-acetyl-alpha-D-galactosamine + glycoprotein-alpha-L-fucosyl-(1->2)-D-galactose = UDP + glycoprotein-N-acetyl-alpha-D-galactosaminyl-(1->3)-[alpha-L-fucosyl-(1->2)]-D-galactose
show the reaction diagram
-
-
-
-
REACTION TYPE
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
hexosyl group transfer
-
-
-
-
PATHWAY
KEGG Link
MetaCyc Link
Glycosphingolipid biosynthesis - lacto and neolacto series
-
Metabolic pathways
-
SYSTEMATIC NAME
IUBMB Comments
UDP-N-acetyl-D-galactosamine:glycoprotein-alpha-L-fucosyl-(1->2)-D-galactose 3-N-acetyl-D-galactosaminyltransferase
Acts on blood group substance, and can use a number of 2-fucosyl-galactosides as acceptors.
SYNONYMS
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
A transferase
-
-
A-transferase
-
-
-
-
ABO(H) blood group A glycosyltransferase
-
-
alpha-(1,3)-N-acetylgalactosaminyltransferase
-
-
alpha-3-N-acetylgalactosaminyltransferase
-
-
-
-
bacterial GTA
-
the enzyme is a GTA homologue
blood group A glycosyltransferase
P16442
-
blood group A glycosyltransferase 2
Q9ET32
-
blood group A glycosyltransferases
-
-
blood-group substance A-dependent acetylgalactosaminyltransferase
-
-
-
-
blood-group substance alpha-acetyltransferase
-
-
-
-
fucosylgalactose acetylgalactosaminyltransferase
-
-
-
-
glycosyltransferases A
-
-
GTA
P16442
-
histo-blood group A acetylgalactosaminyltransferase
-
-
-
-
histo-blood group A glycosyltransferase (Fucalpha1-2Galalpha1-3-N-acetylgalactosaminyltransferase)
-
-
-
-
UDP-GalNAc:Fucalpha1-2Galalpha1-3-N-acetylgalactosaminyltransferase
-
-
-
-
UDP-N-acetyl-D-galactosamine:alpha-L-fucosyl-1,2-D-galactose 3-N-acetyl-D-galactosaminyltransferase
-
-
-
-
histo-blood group A transferase
-
-
-
-
additional information
-
the bacterial enzyme is a homolgue of human blood group A transferase, GTA
additional information
-
the A/B transferases, ABO, belong to the GT6 enzyme family
CAS REGISTRY NUMBER
COMMENTARY
9067-69-0
-
ORGANISM
COMMENTARY
LITERATURE
SEQUENCE CODE
SEQUENCE DB
SOURCE
strain ATCC 43772, gene bgtA
-
-
Manually annotated by BRENDA team
A transferase
-
-
Manually annotated by BRENDA team
blood group A
-
-
Manually annotated by BRENDA team
blood group A1
-
-
Manually annotated by BRENDA team
blood group A1 and A2
-
-
Manually annotated by BRENDA team
blood type A or AB; women
-
-
Manually annotated by BRENDA team
gastric cancer tissue of type 0 individuals, 3 of 31 show A-antigen production
-
-
Manually annotated by BRENDA team
only four amino acids are responsible for B and A blood group enzymes R176G, G235S, L266M and G268A, the latter two amino acids are responsible for the difference in donor specificity and the first two have roles in acceptor binding and turnover
Uniprot
Manually annotated by BRENDA team
only four amino acids are responsible for B and A blood group enzymes R176G, G235S, L266M and G268A, the latter two amino acids are responsible for the difference in donor specificity and the first two have roles in acceptor binding and turnover
-
-
Manually annotated by BRENDA team
crab-eating monkey
-
-
Manually annotated by BRENDA team
Syrian hamster
-
-
Manually annotated by BRENDA team
strain BFM/2Msf, has a cis-AB gene that encodes an enzyme with both A and B transferase activities
Uniprot
Manually annotated by BRENDA team
Mus musculus brevirostris BFM/2Msf
strain BFM/2Msf, has a cis-AB gene that encodes an enzyme with both A and B transferase activities
Uniprot
Manually annotated by BRENDA team
strain CAST/Ei and HMI/Msf, has a cis-AB gene that encodes an enzyme with both A and B transferase activities
Uniprot
Manually annotated by BRENDA team
Mus musculus castaneus CAST/Ei
strain CAST/Ei and HMI/Msf, has a cis-AB gene that encodes an enzyme with both A and B transferase activities
Uniprot
Manually annotated by BRENDA team
strain BALB/c, has a cis-AB gene that encodes an enzyme with both A and B transferase activities
Uniprot
Manually annotated by BRENDA team
Mus musculus domesticus BALB/c
strain BALB/c, has a cis-AB gene that encodes an enzyme with both A and B transferase activities
Uniprot
Manually annotated by BRENDA team
strain ZBN, has a cis-AB gene that encodes an enzyme with both A and B transferase activities
-
-
Manually annotated by BRENDA team
Mus spicilegus ZBN
strain ZBN, has a cis-AB gene that encodes an enzyme with both A and B transferase activities
-
-
Manually annotated by BRENDA team
chimpazee
-
-
Manually annotated by BRENDA team
A-type Abo gene A1; at least 4 different A-type Abo genes; A-type Abo gene A2; at least 4 different A-type Abo genes; A-type Abo gene A3; at least 4 different A-type Abo genes; A-type Abo gene A4; at least 4 different A-type Abo genes
SwissProt
Manually annotated by BRENDA team
SUBSTRATE
PRODUCT                      
REACTION DIAGRAM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
(Substrate)
LITERATURE
(Substrate)
COMMENTARY
(Product)
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
UDP-N-acetyl-alpha-D-galactosamine + 2'-fucosyllactose
UDP + N-acetyl-alpha-D-galactosaminyl-(1,3)-[2'-fucosyllactose]
show the reaction diagram
Q9ET32
-
-
-
?
UDP-N-acetyl-D-galactosamine + 2'-fucosyllactose
UDP + N-acetyl-alpha-D-galactosaminyl-2'-fucosyllactose
show the reaction diagram
-
-
-
-
?
UDP-N-acetyl-D-galactosamine + 2'-fucosyllactose
UDP + N-acetyl-alpha-D-galactosaminyl-2'-fucosyllactose
show the reaction diagram
-
-
-
-
?
UDP-N-acetyl-D-galactosamine + 2'-fucosyllactose
UDP + N-acetyl-alpha-D-galactosaminyl-2'-fucosyllactose
show the reaction diagram
-
-
-
-
?
UDP-N-acetyl-D-galactosamine + alpha-L-fucosyl-1-2-D-galactosyl-O-R
UDP + N-acetyl-alpha-D-galactosaminyl-1,3-[alpha-L-fucosyl-1-2]-D-galactosyl-O-R
show the reaction diagram
-
H-antigen disaccharide, H-antigen disaccharide, Leu226 is responsible for substrate specificity for UDP-N-acetyl-D-galactosamine as donor substrate, acceptor substrate binding pocket structure, overview
-
-
-
UDP-N-acetyl-D-galactosamine + alpha-L-Fucp-(1,2)-beta-D-Galp-(1,3)-beta-DGlcNAcp-O(CH2)7CH3
?
show the reaction diagram
-
41% of the activity with alpha-L-Fucp-(1,2)-beta-D-Galp-O(CH2)7CH3
-
-
?
UDP-N-acetyl-D-galactosamine + alpha-L-Fucp-(1,2)-beta-D-Galp-(1,4)-beta-D-GlcNAcp-O-(CH2)8CO2CH3
?
show the reaction diagram
-
79% of the activity with alpha-L-Fucp-(1,2)-beta-D-Galp-O(CH2)7CH3
-
-
?
UDP-N-acetyl-D-galactosamine + alpha-L-Fucp-(1,2)-beta-D-Galp-O(CH2)7CH3
UDP + alpha-D-GalNAc-(1,3)-[alpha-(Fucp-(1,2)])-beta-D-Galp-O(CH2)7CH3
show the reaction diagram
-
-
-
-
?
UDP-N-acetyl-D-galactosamine + asialo-porcine submaxillary mucin of A-negative blood-type
UDP + N-acetyl-alpha-D-galactosaminyl-1,3-[asialo-porcine submaxillary mucin of A-negative blood-type]
show the reaction diagram
-
-
-
-
?
UDP-N-acetyl-D-galactosamine + glycoprotein alpha-L-fucosyl-1,2-D-galactose
UDP + glycoprotein N-acetyl-alpha-D-galactosaminyl-1,3-[alpha-L-fucosyl-1,2]-D-galactose
show the reaction diagram
-
-
-
-
?
UDP-N-acetyl-D-galactosamine + glycoprotein alpha-L-fucosyl-1,2-D-galactose
UDP + glycoprotein N-acetyl-alpha-D-galactosaminyl-1,3-[alpha-L-fucosyl-1,2]-D-galactose
show the reaction diagram
-
-
-
?
UDP-N-acetyl-D-galactosamine + glycoprotein alpha-L-fucosyl-1,2-D-galactose
UDP + glycoprotein N-acetyl-alpha-D-galactosaminyl-1,3-[alpha-L-fucosyl-1,2]-D-galactose
show the reaction diagram
-
-
-
?
UDP-N-acetyl-D-galactosamine + glycoprotein alpha-L-fucosyl-1,2-D-galactose
UDP + glycoprotein N-acetyl-alpha-D-galactosaminyl-1,3-[alpha-L-fucosyl-1,2]-D-galactose
show the reaction diagram
-
-
-
?
UDP-N-acetyl-D-galactosamine + glycoprotein alpha-L-fucosyl-1,2-D-galactose
UDP + glycoprotein N-acetyl-alpha-D-galactosaminyl-1,3-[alpha-L-fucosyl-1,2]-D-galactose
show the reaction diagram
-
-
-
?
UDP-N-acetyl-D-galactosamine + glycoprotein alpha-L-fucosyl-1,2-D-galactose
UDP + glycoprotein N-acetyl-alpha-D-galactosaminyl-1,3-[alpha-L-fucosyl-1,2]-D-galactose
show the reaction diagram
-
-
-
?
UDP-N-acetyl-D-galactosamine + glycoprotein alpha-L-fucosyl-1,2-D-galactose
UDP + glycoprotein N-acetyl-alpha-D-galactosaminyl-1,3-[alpha-L-fucosyl-1,2]-D-galactose
show the reaction diagram
-
-
-
?
UDP-N-acetyl-D-galactosamine + glycoprotein alpha-L-fucosyl-1,2-D-galactose
UDP + glycoprotein N-acetyl-alpha-D-galactosaminyl-1,3-[alpha-L-fucosyl-1,2]-D-galactose
show the reaction diagram
-
-
-
-
?
UDP-N-acetyl-D-galactosamine + glycoprotein-alpha-L-fucosyl-(1,2)-D-galactose
UDP + glycoprotein-N-acetyl-alpha-D-galactosaminyl-(1,3)-[alpha-L-fucosyl-(1,2)]-D-galactose
show the reaction diagram
-
-
-
-
?
UDP-N-acetyl-D-galactosamine + glycoprotein-alpha-L-fucosyl-(1,2)-D-galactose
UDP + glycoprotein-N-acetyl-alpha-D-galactosaminyl-(1,3)-[alpha-L-fucosyl-(1,2)]-D-galactose
show the reaction diagram
-
substrate is the histo-blood H group antigen, substrate is the histo-blood H group antigen. Arg176 of the enzyme is not important in determining the sugar specificity of enzyme, while Gly235 is influential and Leu266 and Gly268 are crucial in the determination of GalNAc/galactose specificity
product is the histo-blood A group antigen
-
?
UDP-N-acetyl-D-galactosamine + L-fucosyl-alpha-1,2-beta-galactosyl-O(CH2)7CH3
UDP + alpha-D-galactosaminyl-1,3-[alpha-L-fucosyl-1,2]-beta-D-galactosyl-O(CH2)7CH3
show the reaction diagram
-
-
-
-
-
UDP-N-acetyl-D-galactosamine + L-fucosyl-alpha-1,2-beta-galactosyl-O(CH2)7CH3
UDP + alpha-D-galactosaminyl-1,3-[alpha-L-fucosyl-1,2]-beta-D-galactosyl-O(CH2)7CH3
show the reaction diagram
P16442
-
-
-
?
UDP-N-acetyl-D-galactosamine + L-fucosyl-alpha-1,2-beta-galactosyl-O(CH2)7CH3
UDP + alpha-D-galactosaminyl-1,3-[alpha-L-fucosyl-1,2]-beta-D-galactosyl-O(CH2)7CH3
show the reaction diagram
-
-
-
-
?
UDP-N-acetyl-D-galactosamine + lacto-N-fucopentaose I
UDP + N-acetyl-alpha-D-galactosaminyl-1,3-[lacto-N-fucopentaose I]
show the reaction diagram
-
i.e. Fucalpha(1-2)Galbeta(1-3)GlcNAcbeta(1-3)Galbeta(1-4)Glc
-
-
-
UDP-N-acetyl-D-galactose + 2'-fucosyllactose
UDP + N-acetyl-D-galactosyl-1-3-2'-fucosyllactose
show the reaction diagram
Q9ET32
-
-
-
?
UDP-N-acetyl-D-galactose + H type 3-Sp-biotin
UDP + ?
show the reaction diagram
Q9ET32
H type 3-Sp-biotin is the preferred substrate
-
-
?
UDP-N-acetyl-D-glucosamine + L-fucosyl-alpha-1,2-beta-glucosyl-O(CH2)7CH3
UDP + N-acetyl-alpha-D-glucosaminyl-1,3-[alpha-L-fucosyl-1,2]-beta-D-glucosyl-O(CH2)7CH3
show the reaction diagram
P16442
-
-
-
-
UDP-N-acetyl-D-glucosamine + L-fucosyl-alpha-1,2-beta-glucosyl-O(CH2)7CH3
UDP + N-acetyl-alpha-D-glucosaminyl-1,3-[alpha-L-fucosyl-1,2]-beta-D-glucosyl-O(CH2)7CH3
show the reaction diagram
-
-
-
-
?
UDP-N-acetylgalactosamine + octyl 3-O-methyl-alpha-L-fucopyranosyl-(1-2)-beta-D-galactopyranoside
UDP + N-acetyl-alpha-D-galactosamine-1,3-[3-O-methyl-alpha-L-fucosyl-1,2]-beta-D-galactosyl-O(CH2)7CH3
show the reaction diagram
-
-
-
-
?
UDP-N-acetylgalactosamine + octyl 4-O-methyl-alpha-L-fucopyranosyl-(1-2)-beta-D-galactopyranoside
UDP + N-acetyl-alpha-D-galactosaminyl-1,3-[4-O-methyl-alpha-L-fucosyl-1,2]-beta-D-galactosyl-O(CH2)7CH3
show the reaction diagram
-
-
-
-
?
UDP-N-acetylgalactosamine + octyl alpha-L-xylo-hexopyranosyl-(1-2)-beta-D-galactopyranoside
UDP + N-acetyl-alpha-D-galactosaminyl-1,3-[alpha-L-xylo-hexopyranosyl-1,2]-beta-D-galactosyl-O(CH2)7CH3
show the reaction diagram
-
-
-
-
?
UDP-N-acetylgalactosamine + octyl beta-D-arabinopyranosyl-(1-2)-beta-D-galactopyranoside
UDP + N-acetyl-alpha-D-galactosaminyl-1,3-[beta-D-arabinopyranosyl-1,2-beta-D-galactosyl-O(CH2)7CH3]
show the reaction diagram
-
-
-
-
?
additional information
?
-
-
enzyme also catalyzes the transfer of galactose in alpha-linkage to 2'-fucosyllactose, the transfer rate of galactose is much lower than that of N-acetylgalactosamine
-
-
-
additional information
?
-
-
effective acceptor substrates contain a subterminal beta-galactosyl residue substituted at the O-2 position with fucose
-
-
-
additional information
?
-
P16442
transfers N-acetylgalactosamine from UDP-N-acetylgalactosamine to H-active structures to form A determinants
-
-
-
additional information
?
-
-
transfers N-acetylgalactosamine from UDP-N-acetylgalactosamine to H-active structures to form A determinants
-
-
-
additional information
?
-
-
the enzyme catalyzes the final step in the enzymatic synthesis of the ABO(H) blood group A antigen, overview, substrate binding structure by wild-type and mutant GTA, overview
-
-
-
additional information
?
-
-
the enzyme possesses flexible substrate specificity
-
-
-
additional information
?
-
-
galactose is used as an acceptor analogue and UDP as a donor analogue in all soaking trials
-
-
-
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
(Substrate)
LITERATURE
(Substrate)
COMMENTARY
(Product)
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
UDP-N-acetyl-D-galactosamine + alpha-L-fucosyl-1-2-D-galactosyl-O-R
UDP + N-acetyl-alpha-D-galactosaminyl-1,3-[alpha-L-fucosyl-1-2]-D-galactosyl-O-R
show the reaction diagram
-
H-antigen disaccharide
-
-
-
UDP-N-acetyl-D-galactosamine + glycoprotein-alpha-L-fucosyl-(1,2)-D-galactose
UDP + glycoprotein-N-acetyl-alpha-D-galactosaminyl-(1,3)-[alpha-L-fucosyl-(1,2)]-D-galactose
show the reaction diagram
-
-
-
-
?
UDP-N-acetyl-D-galactosamine + glycoprotein-alpha-L-fucosyl-(1,2)-D-galactose
UDP + glycoprotein-N-acetyl-alpha-D-galactosaminyl-(1,3)-[alpha-L-fucosyl-(1,2)]-D-galactose
show the reaction diagram
-
substrate is the histo-blood H group antigen
product is the histo-blood A group antigen
-
?
additional information
?
-
P16442
transfers N-acetylgalactosamine from UDP-N-acetylgalactosamine to H-active structures to form A determinants
-
-
-
additional information
?
-
-
transfers N-acetylgalactosamine from UDP-N-acetylgalactosamine to H-active structures to form A determinants
-
-
-
additional information
?
-
-
the enzyme catalyzes the final step in the enzymatic synthesis of the ABO(H) blood group A antigen, overview
-
-
-
METALS and IONS
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
Mn2+
-
maximal activity with 10 mM Mn2+
Mn2+
-
15-30 mM required for maximal activity
Mn2+
-
cofactor
INHIBITORS
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
IMAGE
2'-fucosyllactose
-
substrate inhibition above 1 mM
alpha-L-fucosyl-1-2-beta-D-(3-amino)-galactosyl-O-R
-
complex mode inhibitor, binding structure, Leu226 is involved by interacting with the C3-position group
alpha-L-fucosyl-1-2-beta-D-(3-deoxy)-galactosyl-O-R
-
competitive inhibitor, binding structure, Leu226 is involved by interacting with the C3-position group
Fluorescein mercuriacetate
-
-
lacto-N-fucopentaose I
-
substrate inhibition above 2 mM
N-ethylmaleimide
-
weak
octyl 3'-amino-3'-deoxy-alpha-L-fucopyranosyl-(1-2)-beta-D-galactopyranoside
-
98% inhibition at 0.025 mM
octyl 3-O-methyl-alpha-L-fucopyranosyl-(1-2)-beta-D-galactopyranoside
-
-
octyl alpha-L-fucopyranosyl-(1-2)-beta-D-gulopyranoside
-
36% inhibition at 0.025 mM
UDP-galactose
-
-
KM VALUE [mM]
KM VALUE [mM] Maximum
SUBSTRATE
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
IMAGE
0.27
-
2'-fucosyllactose
-
-
0.39
-
2'-fucosyllactose
-
distal colon
0.47
-
2'-fucosyllactose
-
pancreatic cancer cell line
1.3
-
alpha-L-Fucp-(1,2)-beta-D-Galp-(1,3)-beta-DGlcNAcp-O(CH2)7CH3
-
37C, pH 7.0
0.49
-
alpha-L-Fucp-(1,2)-beta-D-Galp-(1,4)-beta-D-GlcNAcp-O-(CH2)8CO2CH3
-
37C, pH 7.0
0.087
-
alpha-L-Fucp-(1,2)-beta-D-Galp-O(CH2)7CH3
-
37C, pH 7.0
0.0087
-
L-fucosyl-alpha-1,2-beta-galactosyl-O(CH2)7CH3
P16442
-
0.013
-
L-fucosyl-alpha-1,2-beta-galactosyl-O(CH2)7CH3
-
-
0.144
-
L-fucosyl-alpha-1,2-beta-galactosyl-O(CH2)7CH3
P16442
-
0.167
-
L-fucosyl-alpha-1,2-beta-galactosyl-O(CH2)7CH3
P16442
G176R/P234S/S235G/M266L/A268G mutant
0.02
-
octyl 3-O-methyl-alpha-L-fucopyranosyl-(1-2)-beta-D-galactopyranoside
-
-
1.14
-
octyl 4-O-methyl-alpha-L-fucopyranosyl-(1-2)-beta-D-galactopyranoside
-
-
0.208
-
octyl beta-D-arabinopyranosyl-(1-2)-beta-D-galactopyranoside
-
-
0.0099
-
UDP-GalNAc
P16442
-
0.013
-
UDP-GalNAc
-
-
0.015
-
UDP-GalNAc
-
-
0.048
-
UDP-GalNAc
-
pancreatic cancer cell line, distal colon
3.74
-
UDP-GalNAc
P16442
G176R/P234S/S235G/M266L/A268G mutant
0.242
-
UDP-GlcNAc
P16442
-
0.35
-
lacto-N-fucopentaose I
-
-
additional information
-
additional information
-
kinetics of recombinant wild-type GTA 53-354 and of GTA/GTB mutant chimeric enzymes
-
TURNOVER NUMBER [1/s]
TURNOVER NUMBER MAXIMUM[1/s]
SUBSTRATE
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
IMAGE
1.3
-
alpha-L-Fucp-(1,2)-beta-D-Galp-(1,3)-beta-DGlcNAcp-O(CH2)7CH3
-
37C, pH 7.0
1.8
-
alpha-L-Fucp-(1,2)-beta-D-Galp-(1,4)-beta-D-GlcNAcp-O-(CH2)8CO2CH3
-
37C, pH 7.0
7.6
-
alpha-L-Fucp-(1,2)-beta-D-Galp-O(CH2)7CH3
-
37C, pH 7.0
0.000333
-
UDP-Gal
-
-
0.0817
-
UDP-GalNAc
-
-
0.24
-
UDP-GalNAc
P16442
G176R/P234S/S235G/M266L/A268G mutant
0.292
-
UDP-GalNAc
P16442
wild-type
0.00035
-
UDP-GlcNAc
-
-
Ki VALUE [mM]
Ki VALUE [mM] Maximum
INHIBITOR
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
IMAGE
0.0002
-
octyl 3'-amino-3'-deoxy-alpha-L-fucopyranosyl-(1-2)-beta-D-galactopyranoside
-
-
1
-
octyl 3-O-methyl-alpha-L-fucopyranosyl-(1-2)-beta-D-galactopyranoside
-
-
0.022
-
octyl alpha-L-fucopyranosyl-(1-2)-beta-D-gulopyranoside
-
-
SPECIFIC ACTIVITY [µmol/min/mg]
SPECIFIC ACTIVITY MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
0.2
-
-
plasma
5.7
-
-
lung
30
-
-
-
additional information
-
-
-
pH OPTIMUM
pH MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
6
-
-
assay at
6.4
-
-
blood group A1
6.5
7
-
-
6.6
-
-
-
7
7.4
-
-
7
-
-
pancreatic cancer cell line, antrum and distal colon
7.6
-
-
blood group A2
TEMPERATURE OPTIMUM
TEMPERATURE OPTIMUM MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
37
-
-
assay at
37
-
-
assay at
SOURCE TISSUE
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
SOURCE
-
blood group A1 and A2
Manually annotated by BRENDA team
-
blood group A1
Manually annotated by BRENDA team
Q9ET32
A-type enzyme expression
Manually annotated by BRENDA team
Q9ET32
A-type enzyme expression
Manually annotated by BRENDA team
-
middle and distal
Manually annotated by BRENDA team
Q9ET32
A-type enzyme expression
Manually annotated by BRENDA team
Q9ET32
A-type enzyme expression
Manually annotated by BRENDA team
Q9ET32
A-type enzyme expression
Manually annotated by BRENDA team
Q9ET32
A-type enzyme expression
Manually annotated by BRENDA team
Q9ET32
A-type enzyme expression
Manually annotated by BRENDA team
Q9ET32
A-type enzyme expression
Manually annotated by BRENDA team
Q9ET32
A-type enzyme expression
Manually annotated by BRENDA team
-
produces blood group-A antigen only in pancreatic cancer cells, induced by N-nitrosobis(2-oxopropyl)amine
Manually annotated by BRENDA team
Q9ET32
A-type enzyme expression
Manually annotated by BRENDA team
Q9ET32
A-type enzyme expression
Manually annotated by BRENDA team
-
gastric cancer tissue of type 0 individuals
Manually annotated by BRENDA team
Q9ET32
A-type enzyme expression
Manually annotated by BRENDA team
Q9ET32
A-type enzyme expression
Manually annotated by BRENDA team
Q9ET32
A-type enzyme expression
Manually annotated by BRENDA team
Mus spicilegus ZBN
-
-
-
Manually annotated by BRENDA team
Q9ET32
A-type enzyme expression
Manually annotated by BRENDA team
Q9ET32
A-type enzyme expression
Manually annotated by BRENDA team
LOCALIZATION
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
GeneOntology No.
LITERATURE
SOURCE
PDB
SCOP
CATH
ORGANISM
Bacteroides ovatus (strain ATCC 8483 / DSM 1896 / JCM 5824 / NCTC 11153)
Bacteroides ovatus (strain ATCC 8483 / DSM 1896 / JCM 5824 / NCTC 11153)
Bacteroides ovatus (strain ATCC 8483 / DSM 1896 / JCM 5824 / NCTC 11153)
Bacteroides ovatus (strain ATCC 8483 / DSM 1896 / JCM 5824 / NCTC 11153)
Bacteroides ovatus (strain ATCC 8483 / DSM 1896 / JCM 5824 / NCTC 11153)
MOLECULAR WEIGHT
MOLECULAR WEIGHT MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
90000
100000
-
gel filtration
100000
-
-
zonal centrifugation, gel filtration
SUBUNITS
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
?
-
x * 35000, SDS-PAGE with and without 2-mercaptoethanol
?
-
x * 40000, SDS-PAGE
dimer
-
2 * 52000, SDS-PAGE of carboxymaleyl enzyme
dimer
-
2 * 46000, sedimentation equilibrium of reduced and carboxymethylated enzyme
additional information
-
structure analysis of GTA/GTB chimeric enzymes GTB/G176R and GTB/G176R/G235S bound to a panel of donor and acceptor analogue substrates, open, semi-closed, and closed conformations, overview
POSTTRANSLATIONAL MODIFICATION
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
glycoprotein
-
N-linked carbohydrate chains
glycoprotein
-
-
Crystallization/COMMENTARY
ORGANISM
UNIPROT ACCESSION NO.
LITERATURE
a large single crystal is subjected to H/D exchange prior to data collection and time-of-flight neutron diffraction data is collected to 2.5 A resolution at the Protein Crystallography Station to 85% overall completeness, with complementary X-ray diffraction data collected from a crystal from the same drop and extending to 1.85 A resolution
-
crystals of purified native enzyme are soaked with various combinations of UDP-GalNAc, UDP-Gal, UDP, and acceptor analogues alpha-L-fucosyl-1-2-beta-D-(3-deoxy)-galactosyl-O-R or alpha-L-fucosyl-1-2-beta-D-(3-amino)-galactosyl-O-R, ligands are solved in 7.5% PEG 4000, 15% glycerol, 75 mM N-[2-acetamido]-2-iminodiacetic acid, pH 7.5, 10 mM MnCl2, and 10 mM inhibitor, 3-4 days, X-ray diffraction structure determination and analysis at 2.1 A resolution
-
enzyme soaked with acceptor analogs: galactose, lactose, N-acetyllactosamine, beta-D-Galp-O(CH2)8CO2CH3, alpha-L-Fucp-(1,2)-beta-D-Galp-O(CH2)7CH3, beta-D-Galp-(1,4)-beta-D-Glcp-OCH3, alpha-L-Fucp-(1,2)-beta-D-Galp-(1,3)-beta-D-GlcNAcp-O(CH2)7CH3, alpha-L-Fucp-(1,2)-beta-D-Galp-(1,4)-beta-D-GlcNAcp-O-(CH2)8CO2CH3
-
G176R/P234S/S235G/M266L/A268G-mutant with and without H-antigen, at 1.55 and 1.65 A resolution respectively; of catalytic domain residues 63-354, with and without L-fucosyl-alpha-1,2-beta-galactosyl-O(CH2)7CH3-acceptor and UDP, at 1.35 and 1.8 A resolution respectively
P16442
purified recombinant GTA/GTB mutant chimeric enzymes, complexing with synthetic antigen disaccharides or UDP, hanging drop vapour diffusion method, different solutions for the different chimeric mutants, X-ray diffraction structure determinationand analysis at 1.41-1.75 A resolution, overview, structure modelling
-
structures of GTA, GTB and several chimeras determined by single-crystal X-ray diffraction demonstrate a range of susceptibility to the choice of cryoprotectant, in which the mobile polypeptide loops can be induced by glycerol to form the ordered closed conformation associated with substrate recognition and by MPD (hexylene glycol, 2-methyl-2,4-pentanediol) to hinder binding of substrate in the active site owing to chelation of the Mn2+ cofactor and thereby adopt the disordered open state
-
TEMPERATURE STABILITY
TEMPERATURE STABILITY MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
37
-
-
30 min, 5% loss of activity
GENERAL STABILITY
ORGANISM
UNIPROT ACCESSION NO.
LITERATURE
enzyme is not stable in crude plasma
-
irreversible inactivation in absence of Triton X-100
-
loss of activity after freezing in buffer of pH 7.5
-
STORAGE STABILITY
ORGANISM
UNIPROT ACCESSION NO.
LITERATURE
-20C, rapid loss of activity
-
-60C, 2 mM MnCl2, 1 mM EDTA, 0.02 mM UDP, stable for up to 30 days
-
-80C, 20% loss of activity after 1 year
-
4C, 0.05 M Tris-HCl, pH 7.4, 2 mM MnCl2, 1 mM EDTA, 0.01% w/v Triton X-100, 0.2 M NaCl, 1 mM dithiothreitol, 0.03% w/v NaN3, 10% loss of activity per month
-
4C, 25% loss of activity after storage of plasma for 2 weeks
-
4C, rapid loss of activity of purified enzyme
-
-70C, 0.025 M MES buffer, pH 6.0, 1 mM EDTA, 0.03% Triton X-100, after removal of most of the buffer by dialysis, stable for 1 year
-
4C, pH 7.5, half-life is about 3 months
-
Purification/COMMENTARY
ORGANISM
UNIPROT ACCESSION NO.
LITERATURE
blood group A
-
blood group A; partial
-
recombinant wild-type GTA 53-354 and of GTA/GTB mutant chimeric enzymes from Escherichia coli
-
using a two-step protocol consisting of ion-exchange chromatography followed by UDP-hexanolamine affinity chromatography
-
via ELISA
-
Cloned/COMMENTARY
ORGANISM
UNIPROT ACCESSION NO.
LITERATURE
expressed in Escherichia coli
-
expressed in Escherichia coli BL21, P234S-mutant of blood-group substance B-dependent galactosyltransferase
P16442
expressed in Escherichia coli TG-1
-
expression of truncated wild-type GTA 53-354 and of GTA/GTB mutant chimeric enzymes in Escherichia coli
-
human GTA with the N-terminal transmembrane domain truncated at residue Met63 and codon-optimized for BL21 Escherichia coli is expressed and purified from cultures using a two-step protocol consisting of ion-exchange chromatography followed by UDP-hexanolamine affinity chromatography
-
transfected into HeLa cells derived from human adenocarcinoma of uterus
P38649
transfected into HeLa cells derived from human adenocarcinoma of uterus
-
A-type Abo gene A1, DNA and amino acid sequence determination and analysis, functional expression in CHO cells expressing the H precursor; A-type Abo gene A2, DNA and amino acid sequence determination and analysis, functional expression in CHO cells expressing the H precursor; A-type Abo gene A4, DNA and amino acid sequence determination and analysis; A-type gene Abo A3, DNA and amino acid sequence determination and analysis
Q9ET32
expressed in chinese hamster ovary carcinoma cells, already stable transfected with an alpha1,2-fucosyltransferase
-
ENGINEERING
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
G176R/P234S/S235G/M266L/A268G
P16442
expressed in Escherichia coli BL21, although 4 of the mutations correspond to a change from A- to B-blood group, the P234S-mutation shows a remarkable increase in A-donor specificity
additional information
-
construction of GTA/GTB chimeric enzymes GTB/G176R and GTB/G176R/G235S
additional information
-
generation of a chimeric histo-blood group B transferase by replacing the N-acetyl-D-galactosamine recognition domain of human A transferase with the galactose-recognition domain of evolutionarily related murine alpha1,3-galactosyltransferase, the chimeric mutant shows a functional conversion from A to B transferase activity, overview. The AT-MluI-GT construct, which expresses the chimera between the long N-terminal sequence up to the MluI site of human A transferase, and the short C-terminal sequence up to the MluI site of murine a1-3Gal transferase, produces the antigens that react with both the anti-A and the Griffonia simplicifolia Lectin I, GSL-IA4, lectin
additional information
-
chimeric GTA and GTB enzymes are generated (AABB, ABBA and ABBB)
APPLICATION
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
synthesis
-
the enzyme possesses flexible substrate specificity and can therefore be used to synthesize five different types of A antigens with high efficiency, engineering of bacterial cell surface polysaccharides by BgtA, overview