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Information on EC 2.4.1.320 - 1,4-beta-mannosyl-N-acetylglucosamine phosphorylase

for references in articles please use BRENDA:EC2.4.1.320
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IUBMB Comments
The enzyme isolated from the anaerobic bacterium Bacteroides thetaiotaomicron is involved in the degradation of host-derived N-glycans.
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This record set is specific for:
UNIPROT: Q8A8X4
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The expected taxonomic range for this enzyme is: Bacteria, Archaea
Synonyms
bt1033, more
SYNONYM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
PATHWAY SOURCE
PATHWAYS
-
-
SYSTEMATIC NAME
IUBMB Comments
4-O-beta-D-mannopyranosyl-N-acetyl-D-glucosamine:phosphate alpha-D-mannosyltransferase
The enzyme isolated from the anaerobic bacterium Bacteroides thetaiotaomicron is involved in the degradation of host-derived N-glycans.
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
4-O-beta-D-mannosyl-N-acetyl-D-glucosamine + phosphate
N-acetyl-D-glucosamine + alpha-D-mannose 1-phosphate
show the reaction diagram
-
-
-
r
4-O-beta-N-acetyl-D-glucosaminyl-N-acetyl-D-glucosamine + alpha-D-mannose 1-phosphate
beta-N-acetyl-D-glucosaminyl-(1->4)-beta-N-acetyl-D-glucosaminyl-(1->4)-D-mannose + phosphate
show the reaction diagram
-
-
-
r
beta-N-acetyl-D-glucosaminyl-(1->4)-beta-N-acetyl-D-glucosaminyl-(1->4)-D-mannose + phosphate
4-O-beta-N-acetyl-D-glucosaminyl-N-acetyl-D-glucosamine + alpha-D-mannose 1-phosphate
show the reaction diagram
-
-
-
r
N-acetyl-D-glucosamine + alpha-D-mannose 1-phosphate
4-O-beta-D-mannosyl-N-acetyl-D-glucosamine + phosphate
show the reaction diagram
-
-
-
r
additional information
?
-
NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
4-O-beta-D-mannosyl-N-acetyl-D-glucosamine + phosphate
N-acetyl-D-glucosamine + alpha-D-mannose 1-phosphate
show the reaction diagram
-
-
-
r
N-acetyl-D-glucosamine + alpha-D-mannose 1-phosphate
4-O-beta-D-mannosyl-N-acetyl-D-glucosamine + phosphate
show the reaction diagram
-
-
-
r
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
2.7
4-O-beta-N-acetyl-D-glucosaminyl-N-acetyl-D-glucosamine
pH 5.5, 30°C
2.6
alpha-D-mannose 1-phosphate
pH 5.5, 30°C
3.8
N-acetyl-D-glucosamine
pH 5.5, 30°C
TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
22
4-O-beta-N-acetyl-D-glucosaminyl-N-acetyl-D-glucosamine
pH 5.5, 30°C
25
alpha-D-mannose 1-phosphate
pH 5.5, 30°C
37
N-acetyl-D-glucosamine
pH 5.5, 30°C
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
UNIPROT
ENTRY NAME
ORGANISM
NO. OF AA
NO. OF TRANSM. HELICES
MOLECULAR WEIGHT[Da]
SOURCE
SEQUENCE
LOCALIZATION PREDICTION?
Q8A8X4_BACTN
Bacteroides thetaiotaomicron (strain ATCC 29148 / DSM 2079 / JCM 5827 / CCUG 10774 / NCTC 10582 / VPI-5482 / E50)
546
0
60933
TrEMBL
-
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
35000
4 * 35000, SDS-PAGE, 4 * 37823, amino acid sequence
37823
4 * 35000, SDS-PAGE, 4 * 37823, amino acid sequence
SUBUNIT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
homotetramer
4 * 35000, SDS-PAGE, 4 * 37823, amino acid sequence
pH STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
4.5 - 10.5
4°C, 24 h stable
725534
TEMPERATURE STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
expressed in Escherichia coli DH5alpha
REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Nihira, T.; Suzuki, E.; Kitaoka, M.; Nishimoto, M.; Ohtsubo, K.; Nakai, H.
Discovery of beta-1,4-D-mannosyl-N-acetyl-D-glucosamine phosphorylase involved in the metabolism of N-glycans
J. Biol. Chem.
288
27366-27374
2013
Bacteroides thetaiotaomicron (Q8A8X4), Bacteroides thetaiotaomicron (Q8A8Y4), Bacteroides thetaiotaomicron VPI-5482 (Q8A8X4), Bacteroides thetaiotaomicron VPI-5482 (Q8A8Y4), Bacteroides thetaiotaomicron VPI-5482
Manually annotated by BRENDA team