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IUBMB CommentsThe glucosyl group forms a 1->6 bond to the glucosyloxy moiety at C-20 of ginsenoside Rd. Isolated from sanchi ginseng (Panax notoginseng).
The expected taxonomic range for this enzyme is: Panax
Synonyms
ugrdgt, udpg-ginsenoside rd glucosyltransferase, udpg:ginsenoside rd glucosyltransferase,
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UDP-ginsenoside Rd glucosyltransferase
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UDP-glucose:ginsenoside Rd glucosyltransferase
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UDPG-ginsenoside Rd glucosyltransferase
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UDPG:ginsenoside Rd glucosyltransferase
UDPG:ginsenoside Rd glucosyltransferase
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UDPG:ginsenoside Rd glucosyltransferase
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UGRdGT
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UDP-alpha-D-glucose + ginsenoside Rd = UDP + ginsenoside Rb1
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UDP-glucose:ginsenoside-Rd beta-1,6-glucosyltransferase
The glucosyl group forms a 1->6 bond to the glucosyloxy moiety at C-20 of ginsenoside Rd. Isolated from sanchi ginseng (Panax notoginseng).
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UDP-alpha-D-glucose + ginsenoside Rd
UDP + ginsenoside Rb1
UDP-alpha-D-glucose + ginsenoside Rd
UDP + ginsenoside Rb1
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UDP-alpha-D-glucose + ginsenoside Rd
UDP + ginsenoside Rb1
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UDP-alpha-D-glucose + ginsenoside Rd
UDP + ginsenoside Rb1
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over 80% yield after 10 h
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UDP-alpha-D-glucose + ginsenoside Rd
UDP + ginsenoside Rb1
UDP-alpha-D-glucose + ginsenoside Rd
UDP + ginsenoside Rb1
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UDP-alpha-D-glucose + ginsenoside Rd
UDP + ginsenoside Rb1
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UDP-alpha-D-glucose + ginsenoside Rd
UDP + ginsenoside Rb1
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over 80% yield after 10 h
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Ca2+
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129% activity at 5 mM
Mg2+
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111.9% activity at 5 mM
Mn2+
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133.2% activity at 5 mM
additional information
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the activity of the enzyme is not apparently affected by Na+ or K+
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Co2+
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8.2% residual activity at 5 mM
Cu2+
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2.2% residual activity at 5 mM
Fe2+
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2.3% residual activity at 5 mM
Hg2+
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complete inhibition at 5 mM
Zn2+
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complete inhibition at 5 mM
additional information
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the activity of the enzyme is not apparently affected by EDTA
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0.14
ginsenoside Rd
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in 50 mM Tris-HCl buffer, at pH 8.5 and 35°C
0.32
UDP-alpha-D-glucose
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in 50 mM Tris-HCl buffer, at pH 8.5 and 35°C
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0.0003
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crude extract, at pH 8.5 and 35°C
0.044
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after 145.8fold purification, at pH 8.5 and 35°C
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7 - 9
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more than 60% activity between pH 7.0 and 9.0
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brenda
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brenda
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36000
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x * 36000, SDS-PAGE
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ammonium sulfate precipitation, HiTrap Q anion-exchange chromatography, and Sephadex G-25 gel filtration
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6 days after treatment, the enzyme activity is elicited by 0.2 mM 2-hydroxyethyl jasmonate and induced by 0.2 mM methyl jasmonate
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the activity of the enzyme is up-regulated 6 days after treatment with 0.01 mM N,N'-dicyclohexylcarbodiimide
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Hu, F.X.; Zhong, J.J.
Role of jasmonic acid in alteration of ginsenoside heterogeneity in elicited cell cultures of Panax notoginseng
J. Biosci. Bioeng.
104
513-516
2007
Panax notoginseng
brenda
Huang, C.; Qian, Z.G.; Zhong, J.J.
Enhancement of ginsenoside biosynthesis in cell cultures of Panax ginseng by N,N'-dicyclohexylcarbodiimide elicitation
J. Biotechnol.
165
30-36
2013
Panax ginseng
brenda
Yue, C.; Zhong, J.
Purification and characterization of UDPG:ginsenoside Rd glucosyltransferase from suspended cells of Panax notoginseng
Process Biochem.
40
3742-3748
2005
Panax notoginseng
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brenda
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