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Information on EC 2.4.1.311 - chloroorienticin B synthase and Organism(s) Amycolatopsis orientalis and UniProt Accession P96558

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EC Tree
     2 Transferases
         2.4 Glycosyltransferases
             2.4.1 Hexosyltransferases
                2.4.1.311 chloroorienticin B synthase
IUBMB Comments
The enzyme from the bacterium Amycolatopsis orientalis is involved in the biosynthesis of the glycopeptide antibiotic chloroeremomycin.
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This record set is specific for:
Amycolatopsis orientalis
UNIPROT: P96558
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The taxonomic range for the selected organisms is: Amycolatopsis orientalis
The expected taxonomic range for this enzyme is: Amycolatopsis
Synonyms
chloroorienticin B synthase, GtfA, more
SYNONYM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
chloroorienticin B synthase
-
-
-
-
PATHWAY SOURCE
PATHWAYS
SYSTEMATIC NAME
IUBMB Comments
dTDP-L-4-epi-vancosamine:desvancosaminyl-vancomycin vancosaminyltransferase
The enzyme from the bacterium Amycolatopsis orientalis is involved in the biosynthesis of the glycopeptide antibiotic chloroeremomycin.
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
dTDP-beta-L-4-epi-vancosamine + aglucovancomycin
dTDP + chloroorienticin C
show the reaction diagram
low activity
-
-
?
dTDP-beta-L-4-epi-vancosamine + desvancosaminyl-vancomycin
dTDP + chloroorienticin B
show the reaction diagram
-
-
-
?
dTDP-beta-L-4-epi-vancosamine + devancosaminyl-vancomycin
dTDP + chloroorienticin B
show the reaction diagram
dTDP-beta-L-4-epi-vancosamine + epivancomycin
dTDP + chloroeremomycin
show the reaction diagram
low activity
-
-
?
NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
dTDP-beta-L-4-epi-vancosamine + devancosaminyl-vancomycin
dTDP + chloroorienticin B
show the reaction diagram
the enzyme is involved in the biosynthesis of the glycopeptide antibiotic chloroeremomycin
-
-
?
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.107
desvancosaminyl-vancomycin
pH 7.5, 37°C
0.218
dTDP-beta-L-4-epi-vancosamine
pH 7.5, 37°C
TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.038
desvancosaminyl-vancomycin
pH 7.5, 37°C
0.038
dTDP-beta-L-4-epi-vancosamine
pH 7.5, 37°C
additional information
additional information
GtfA transfers 4-epi-vancosamine to vancomycin aglycone or epivancomycin at much reduced rates of less than 0.0008/s
-
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
physiological function
the enzyme is involved in the biosynthesis of chloroeremomycin
UNIPROT
ENTRY NAME
ORGANISM
NO. OF AA
NO. OF TRANSM. HELICES
MOLECULAR WEIGHT[Da]
SOURCE
SEQUENCE
LOCALIZATION PREDICTION?
GTFA_AMYOR
396
0
41662
Swiss-Prot
-
CRYSTALLIZATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
2.8 A crystal structures of GtfA complexes with vancomycin and the natural monoglycosylated peptide substrate devancoaminyl vancomycin
PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
heterologously expressed as a C-terminal His6-tagged protein in Escherichia coli
REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Mulichak, A.M.; Losey, H.C.; Lu, W.; Wawrzak, Z.; Walsh, C.T.; Garavito, R.M.
Structure of the TDP-epi-vancosaminyltransferase GtfA from the chloroeremomycin biosynthetic pathway
Proc. Natl. Acad. Sci. USA
100
9238-9243
2003
Amycolatopsis orientalis (P96558), Amycolatopsis orientalis A82846 (P96558)
Manually annotated by BRENDA team
Lu, W.; Oberthr, M.; Leimkuhler, C.; Tao, J.; Kahne, D.; Walsh, C.T.
Characterization of a regiospecific epivancosaminyl transferase GtfA and enzymatic reconstitution of the antibiotic chloroeremomycin
Proc. Natl. Acad. Sci. USA
101
4390-4395
2004
Amycolatopsis orientalis (P96558), Amycolatopsis orientalis A82846 (P96558)
Manually annotated by BRENDA team