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Enzyme Nomenclature
Information on EC 2.4.1.30 - 1,3-beta-oligoglucan phosphorylase
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The expected taxonomic range for this enzyme is: Euglena gracilis
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EC NUMBER 
COMMENTARY 
2.4.1.30
-
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RECOMMENDED NAME
GeneOntology No.
1,3-beta-oligoglucan phosphorylase
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REACTION
REACTION DIAGRAM
COMMENTARY
ORGANISM
UNIPROT
LITERATURE
[(1->3)-beta-D-glucosyl]n + phosphate = [(1->3)-beta-D-glucosyl]n-1 + alpha-D-glucose 1-phosphate
[(1->3)-beta-D-glucosyl]n + phosphate = [(1->3)-beta-D-glucosyl]n-1 + alpha-D-glucose 1-phosphate
inversion mechanism
-
[(1->3)-beta-D-glucosyl]n + phosphate = [(1->3)-beta-D-glucosyl]n-1 + alpha-D-glucose 1-phosphate
-
-
-
-
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REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
hexosyl group transfer
-
-
-
-
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SYSTEMATIC NAME
IUBMB Comments
(1->3)-beta-D-glucan:phosphate alpha-D-glucosyltransferase
Does not act on laminarin. Differs in specificity from EC 2.4.1.31 (laminaribiose phosphorylase) and EC 2.4.1.97 (1,3-beta-D-glucan phosphorylase).
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CAS REGISTRY NUMBER
COMMENTARY
37257-28-6
-
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ORGANISM
COMMENTARY
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
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SUBSTRATE
PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
(Substrate)
LITERATURE
(Substrate)
(Substrate)
COMMENTARY
(Product)
(Product)
LITERATURE
(Product)
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
alpha-phenylglucoside + alpha-D-glucose 1-phosphate
1,3-beta-D-glucosyl-alpha-phenylglucoside + phosphate
-
very low activity
-
-
r
arbutin + alpha-D-glucose 1-phosphate
1,3-beta-D-glucosylarbutin + phosphate
-
-
-
-
r
beta-methylglucoside + alpha-D-glucose 1-phosphate
1,3-beta-D-glucosyl-beta-methylglucoside + phosphate
-
-
-
-
r
beta-phenylglucoside + alpha-D-glucose 1-phosphate
1,3-beta-D-glucosyl-beta-phenylglucoside + phosphate
-
-
-
-
r
D-glucose + alpha-D-glucose 1-phosphate
laminaribiose + phosphate
-
-
-
-
r
gentiobiose + alpha-D-glucose 1-phosphate
1,3-beta-D-glucosylgentiobiose + phosphate
-
-
-
-
r
laminaribiosyl-beta-1,4-glucose + alpha-D-glucose 1-phosphate
laminaritriosyl-beta-1,4-glucose + phosphate
-
-
-
-
r
laminaribiosyl-p-hydroquinone + alpha-D-glucose 1-phosphate
laminaritriosyl-p-hydroquinone + phosphate
-
-
-
-
r
laminaribiosyl-saligenin + alpha-D-glucose 1-phosphate
laminaritriosyl-saligenin + phosphate
laminariheptaose + alpha-D-glucose 1-phosphate
laminarioctaose + phosphate
-
-
-
-
r
laminaripentaose + alpha-D-glucose 1-phosphate
laminarihexaose + phosphate
-
low activity
-
-
r
laminaripentaose + phosphate
laminaritetraose + alpha-D-glucose 1-phosphate
-
-
-
-
r
laminaritetraose + alpha-D-glucose 1-phosphate
laminaripentaose + phosphate
-
low activity
-
-
r
laminaritetraose + phosphate
laminaritriose + alpha-D-glucose 1-phosphate
-
-
-
-
r
laminaritetraosyl-saligenin + alpha-D-glucose 1-phosphate
laminaripentaosyl-saligenin + phosphate
laminaritriose + alpha-D-glucose 1-phosphate
laminaritetraose + phosphate
-
-
-
-
r
laminaritriose + phosphate
laminaribiose + alpha-D-glucose 1-phosphate
-
-
-
r
laminaritriosyl-p-hydroquinone + alpha-D-glucose 1-phosphate
laminaritetraosyl-p-hydroquinone + phosphate
laminaritriosyl-saligenin + alpha-D-glucose 1-phosphate
laminaritetraosyl-saligenin + phosphate
salicin + alpha-D-glucose 1-phosphate
1,3-beta-D-glucosylsalicin + phosphate
-
-
-
-
r
(1,3-beta-D-glucosyl)n-1 + alpha-D-glucose 1-phosphate
(1,3-beta-D-glucosyl)n + phosphate
-
-
-
r
(1,3-beta-D-glucosyl)n-1 + alpha-D-glucose 1-phosphate
(1,3-beta-D-glucosyl)n + phosphate
-
-
-
r
(1,3-beta-D-glucosyl)n-1 + alpha-D-glucose 1-phosphate
(1,3-beta-D-glucosyl)n + phosphate
-
-
-
r
(1,3-beta-D-glucosyl)n-1 + alpha-D-glucose 1-phosphate
(1,3-beta-D-glucosyl)n + phosphate
-
-
-
r
(1,3-beta-D-glucosyl)n-1 + alpha-D-glucose 1-phosphate
(1,3-beta-D-glucosyl)n + phosphate
-
role in utilization of the storage polysaccharide paramylon, i.e. beta-1,3-glucan
-
r
(1,3-beta-D-glucosyl)n-1 + alpha-D-glucose 1-phosphate
(1,3-beta-D-glucosyl)n + phosphate
-
-
-
r
(1,3-beta-D-glucosyl)n-1 + alpha-D-glucose 1-phosphate
(1,3-beta-D-glucosyl)n + phosphate
Ochromonas malhamensis
-
-
-
r
laminaribiose + alpha-D-glucose 1-phosphate
laminaritriose + phosphate
-
-
-
r
laminaribiose + alpha-D-glucose 1-phosphate
laminaritriose + phosphate
-
-
product analysis method
r
laminaribiose + phosphate
D-glucose + alpha-D-glucose 1-phosphate
-
-
-
r
laminaribiosyl-saligenin + alpha-D-glucose 1-phosphate
laminaritriosyl-saligenin + phosphate
-
-
-
-
r
laminaribiosyl-saligenin + alpha-D-glucose 1-phosphate
laminaritriosyl-saligenin + phosphate
-
best acceptor
-
-
r
laminaritetraosyl-saligenin + alpha-D-glucose 1-phosphate
laminaripentaosyl-saligenin + phosphate
-
-
-
-
r
laminaritetraosyl-saligenin + alpha-D-glucose 1-phosphate
laminaripentaosyl-saligenin + phosphate
-
low activity
-
-
r
laminaritriosyl-p-hydroquinone + alpha-D-glucose 1-phosphate
laminaritetraosyl-p-hydroquinone + phosphate
-
-
-
-
r
laminaritriosyl-p-hydroquinone + alpha-D-glucose 1-phosphate
laminaritetraosyl-p-hydroquinone + phosphate
-
low activity
-
-
r
laminaritriosyl-saligenin + alpha-D-glucose 1-phosphate
laminaritetraosyl-saligenin + phosphate
-
-
-
-
r
laminaritriosyl-saligenin + alpha-D-glucose 1-phosphate
laminaritetraosyl-saligenin + phosphate
-
low activity
-
-
-
additional information
?
-
-
EC 2.4.1.30 and EC 2.4.1.31 catalyze the same reaction but with different quantitative specificities, EC 2.4.1.30 phosphorolyzes laminaritriose and higher homologues at a greater rate than laminaribiose, while the opposite behavior is observed with EC 2.4.1.31 i.e. laminaribiose phosphorylase
-
-
-
additional information
?
-
-
alpha-methylglucoside and laminarin are no acceptors
-
-
-
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NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
(Substrate)
LITERATURE
(Substrate)
(Substrate)
COMMENTARY
(Product)
(Product)
LITERATURE
(Product)
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
(1,3-beta-D-glucosyl)n-1 + alpha-D-glucose 1-phosphate
(1,3-beta-D-glucosyl)n + phosphate
-
-
-
r
(1,3-beta-D-glucosyl)n-1 + alpha-D-glucose 1-phosphate
(1,3-beta-D-glucosyl)n + phosphate
-
-
-
r
(1,3-beta-D-glucosyl)n-1 + alpha-D-glucose 1-phosphate
(1,3-beta-D-glucosyl)n + phosphate
-
role in utilization of the storage polysaccharide paramylon, i.e. beta-1,3-glucan
-
r
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INHIBITORS
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
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ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
additional information
-
absolute requirement for sulfhydryl groups
-
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KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
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SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
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pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
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TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
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GENERAL STABILITY
ORGANISM
UNIPROT
LITERATURE
without 2-mercaptoethanol: loss of 80% activity after 2 weeks and over 95% after 8 weeks in enzyme extracts, with 2-mercaptoethanol: loss of 50% activity after 8 weeks
-
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Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
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APPLICATION
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
synthesis
synthesis
-
synthesis of laminarioligosaccharides by combined action of laminaribiose phosphorylase EC 2.4.1.31 and beta-1,3-oligoglucan phosphorylase EC 2.4.1.30
synthesis
-
synthesis of laminarioligosaccharides by combined action of laminaribiose phosphorylase EC 2.4.1.31 and beta-1,3-oligoglucan phosphorylase EC 2.4.1.30; synthesis of laminarioligosaccharides, degree of polymerization is dependent on the glucose/glucose 1-phosphate ratio, overview
synthesis
-
synthesis of laminarioligosaccharides by combined action of laminaribiose phosphorylase EC 2.4.1.31 and beta-1,3-oligoglucan phosphorylase EC 2.4.1.30
-
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LINKS TO OTHER DATABASES (specific for EC-Number 2.4.1.30)
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