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4-O-beta-D-mannopyranosyl-D-glucopyranose + phosphate
D-glucose + alpha-D-mannose 1-phosphate
-
-
-
r
6-deoxy-6-fluoro-D-glucose + alpha-D-mannose 1-phosphate
4-O-beta-D-mannopyranosyl-6-deoxy-6-fluoro-D-glucopyranose + phosphate
-
-
-
?
6-deoxy-D-glucose + alpha-D-mannose 1-phosphate
4-O-beta-D-mannopyranosyl-6-deoxy-D-glucopyranose + phosphate
-
-
-
?
D-glucose + alpha-D-mannose 1-phosphate
4-O-beta-D-mannopyranosyl-D-glucopyranose + phosphate
laminaribiose + alpha-D-mannose 1-phosphate
? + phosphate
-
-
-
?
1,5-anhydro-D-glucitol + alpha-D-mannose 1-phosphate
4-O-beta-D-mannopyranosyl-1,5-anhydro-D-glucitol + phosphate
-
-
-
r
2-deoxy-2-fluoro-D-glucose + alpha-D-mannose 1-phosphate
4-O-beta-D-mannopyranosyl-2-deoxy-2-fluoro-D-glucopyranose + phosphate
-
-
-
r
2-deoxy-D-glucose + alpha-D-mannose 1-phosphate
4-O-beta-D-mannopyranosyl-2-deoxy-D-glucopyranose + phosphate
-
-
-
r
3-deoxy-3-fluoro-D-glucose + alpha-D-mannose 1-phosphate
4-O-beta-D-mannopyranosyl-3-deoxy-3-fluoro-D-glucopyranose + phosphate
-
-
-
r
3-O-methyl-D-glucose + alpha-D-mannose 1-phosphate
4-O-beta-D-mannopyranosyl-3-O-methyl-D-glucopyranose + phosphate
-
-
-
r
4-O-beta-D-mannopyranosyl-D-glucopyranose + phosphate
D-glucose + alpha-D-mannose 1-phosphate
-
-
-
r
cellobiose + alpha-D-mannose 1-phosphate
? + phosphate
-
-
-
r
D-allose + alpha-D-mannose 1-phosphate
4-O-beta-D-mannopyranosyl-D-allose + phosphate
-
-
-
r
D-glucose + alpha-D-mannose 1-phosphate
4-O-beta-D-mannopyranosyl-D-glucopyranose + phosphate
-
-
-
r
D-mannose + alpha-D-mannose 1-phosphate
4-O-beta-D-mannopyranosyl-D-mannopyranose + phosphate
-
-
-
r
gentiobiose + alpha-D-mannose 1-phosphate
? + phosphate
-
-
-
r
laminaribiose + alpha-D-mannose 1-phosphate
? + phosphate
-
-
-
r
maltose + alpha-D-mannose 1-phosphate
? + phosphate
-
-
-
r
methyl alpha-D-glucoside + alpha-D-mannose 1-phosphate
methyl [4-O-beta-D-mannopyranosyl]-alpha-D-glucoside + phosphate
-
-
-
r
methyl beta-D-glucoside + alpha-D-mannose 1-phosphate
methyl [4-O-beta-D-mannopyranosyl]-beta-D-glucopyranoside + phosphate
-
-
-
r
N,N'-diacetylchitobiose + alpha-D-mannose 1-phosphate
? + phosphate
-
-
-
r
N-acetyl-D-glucosamine + alpha-D-mannose 1-phosphate
4-O-beta-D-mannopyranosyl-N-acetyl-D-glucosamine + phosphate
-
-
-
r
sophorose + alpha-D-mannose 1-phosphate
? + phosphate
-
-
-
r
additional information
?
-
D-glucose + alpha-D-mannose 1-phosphate
4-O-beta-D-mannopyranosyl-D-glucopyranose + phosphate
-
-
-
?
D-glucose + alpha-D-mannose 1-phosphate
4-O-beta-D-mannopyranosyl-D-glucopyranose + phosphate
-
-
-
r
additional information
?
-
The enzyme also catalyzes the reaction of beta-1,4-mannooligosaccharide phosphorylase
-
-
?
additional information
?
-
The enzyme also catalyzes the reaction of beta-1,4-mannooligosaccharide phosphorylase
-
-
?
additional information
?
-
-
The enzyme also catalyzes the reaction of beta-1,4-mannooligosaccharide phosphorylase
-
-
?
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0.304
4-O-beta-D-mannopyranosyl-D-glucopyranose
pH 6.5, 37°C, phosphorolytic reaction, cosubstrate phosphate
156
6-deoxy-6-fluoro-D-glucose
pH 6.5, 37°C, synthetic reaction, cosubstrate alpha-D-mannose 1-phosphate
119
6-deoxy-D-glucose
pH 6.5, 37°C, synthetic reaction, cosubstrate alpha-D-mannose 1-phosphate
2.41 - 36.5
alpha-D-mannose 1-phosphate
5.34
phosphate
pH 6.5, 37°C, phosphorolytic reaction, cosubstrate 4-O-beta-D-mannopyranosyl-D-glucopyranose
79.2
1,5-anhydro-D-glucitol
apparent value, pH 6.5, 37°C, synthetic reaction, cosubstrate alpha-D-mannose 1-phosphate
48.7
2-deoxy-2-fluoro-D-glucose
apparent value, pH 6.5, 37°C, synthetic reaction, cosubstrate alpha-D-mannose 1-phosphate
90.7
2-deoxy-D-glucose
apparent value, pH 6.5, 37°C, synthetic reaction, cosubstrate alpha-D-mannose 1-phosphate
6.44
3-deoxy-3-fluoro-D-glucose
apparent value, pH 6.5, 37°C, synthetic reaction, cosubstrate alpha-D-mannose 1-phosphate
7.82
3-O-methyl-D-glucose
apparent value, pH 6.5, 37°C, synthetic reaction, cosubstrate alpha-D-mannose 1-phosphate
27.7
4-O-beta-D-mannopyranosyl-D-glucopyranose
pH 6.5, 37°C, phosphorolytic reaction, cosubstrate phosphate
0.324
alpha-D-mannose 1-phosphate
pH 6.5, 37°C, synthetic reaction, cosubstrate D-glucose
22.2
cellobiose
apparent value, pH 6.5, 37°C, synthetic reaction, cosubstrate alpha-D-mannose 1-phosphate
6.14
D-allose
apparent value, pH 6.5, 37°C, synthetic reaction, cosubstrate alpha-D-mannose 1-phosphate
172
D-glucose
apparent value, pH 6.5, 37°C, synthetic reaction, cosubstrate alpha-D-mannose 1-phosphate
45.6
D-mannose
apparent value, pH 6.5, 37°C, synthetic reaction, cosubstrate alpha-D-mannose 1-phosphate
60.8
gentiobiose
apparent value, pH 6.5, 37°C, synthetic reaction, cosubstrate alpha-D-mannose 1-phosphate
29.1
laminaribiose
apparent value, pH 6.5, 37°C, synthetic reaction, cosubstrate alpha-D-mannose 1-phosphate
50.4
maltose
apparent value, pH 6.5, 37°C, synthetic reaction, cosubstrate alpha-D-mannose 1-phosphate
85.3
methyl alpha-D-glucoside
apparent value, pH 6.5, 37°C, synthetic reaction, cosubstrate alpha-D-mannose 1-phosphate
85.2
methyl beta-D-glucoside
apparent value, pH 6.5, 37°C, synthetic reaction, cosubstrate alpha-D-mannose 1-phosphate
49.9
N,N'-diacetylchitobiose
apparent value, pH 6.5, 37°C, synthetic reaction, cosubstrate alpha-D-mannose 1-phosphate
107
N-acetyl-D-glucosamine
apparent value, pH 6.5, 37°C, synthetic reaction, cosubstrate alpha-D-mannose 1-phosphate
0.444
phosphate
pH 6.5, 37°C, phosphorolytic reaction, cosubstrate 4-O-beta-D-mannopyranosyl-D-glucopyranose
49.8
sophorose
apparent value, pH 6.5, 37°C, synthetic reaction, cosubstrate alpha-D-mannose 1-phosphate
2.41
alpha-D-mannose 1-phosphate
wild-type, pH 6.5, 37°C
5.8
alpha-D-mannose 1-phosphate
pH 6.5, 37°C, synthetic reaction, cosubstrate D-glucose
36.5
alpha-D-mannose 1-phosphate
mutant H245A, pH 6.5, 37°C
25.9
D-glucose
wild-type, pH 6.5, 37°C
38.2
D-glucose
pH 6.5, 37°C, synthetic reaction, cosubstrate alpha-D-mannose 1-phosphate
319
D-glucose
mutant H245A, pH 6.5, 37°C
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83.3
4-O-beta-D-mannopyranosyl-D-glucopyranose
pH 6.5, 37°C, phosphorolytic reaction, cosubstrate phosphate
42
6-deoxy-6-fluoro-D-glucose
pH 6.5, 37°C, synthetic reaction, cosubstrate alpha-D-mannose 1-phosphate
177
6-deoxy-D-glucose
pH 6.5, 37°C, synthetic reaction, cosubstrate alpha-D-mannose 1-phosphate
2.76 - 52.1
alpha-D-mannose 1-phosphate
20.7
1,5-anhydro-D-glucitol
apparent value, pH 6.5, 37°C, synthetic reaction, cosubstrate alpha-D-mannose 1-phosphate
5.71
2-deoxy-2-fluoro-D-glucose
apparent value, pH 6.5, 37°C, synthetic reaction, cosubstrate alpha-D-mannose 1-phosphate
11.7
2-deoxy-D-glucose
apparent value, pH 6.5, 37°C, synthetic reaction, cosubstrate alpha-D-mannose 1-phosphate
0.94
3-deoxy-3-fluoro-D-glucose
apparent value, pH 6.5, 37°C, synthetic reaction, cosubstrate alpha-D-mannose 1-phosphate
0.763
3-O-methyl-D-glucose
apparent value, pH 6.5, 37°C, synthetic reaction, cosubstrate alpha-D-mannose 1-phosphate
17.5
4-O-beta-D-mannopyranosyl-D-glucopyranose
pH 6.5, 37°C, phosphorolytic reaction, cosubstrate phosphate
20.2
cellobiose
apparent value, pH 6.5, 37°C, synthetic reaction, cosubstrate alpha-D-mannose 1-phosphate
0.667
D-allose
apparent value, pH 6.5, 37°C, synthetic reaction, cosubstrate alpha-D-mannose 1-phosphate
5.12
D-mannose
apparent value, pH 6.5, 37°C, synthetic reaction, cosubstrate alpha-D-mannose 1-phosphate
26.1
gentiobiose
apparent value, pH 6.5, 37°C, synthetic reaction, cosubstrate alpha-D-mannose 1-phosphate
11.6
laminaribiose
apparent value, pH 6.5, 37°C, synthetic reaction, cosubstrate alpha-D-mannose 1-phosphate
5.07
maltose
apparent value, pH 6.5, 37°C, synthetic reaction, cosubstrate alpha-D-mannose 1-phosphate
20.7
methyl alpha-D-glucoside
apparent value, pH 6.5, 37°C, synthetic reaction, cosubstrate alpha-D-mannose 1-phosphate
35.2
methyl beta-D-glucoside
apparent value, pH 6.5, 37°C, synthetic reaction, cosubstrate alpha-D-mannose 1-phosphate
6.34
N,N'-diacetylchitobiose
apparent value, pH 6.5, 37°C, synthetic reaction, cosubstrate alpha-D-mannose 1-phosphate
15.8
N-acetyl-D-glucosamine
apparent value, pH 6.5, 37°C, synthetic reaction, cosubstrate alpha-D-mannose 1-phosphate
13.8
sophorose
apparent value, pH 6.5, 37°C, synthetic reaction, cosubstrate alpha-D-mannose 1-phosphate
2.76
alpha-D-mannose 1-phosphate
mutant H245A, pH 6.5, 37°C
52.1
alpha-D-mannose 1-phosphate
wild-type, pH 6.5, 37°C
19.3
D-glucose
mutant H245A, pH 6.5, 37°C
45.1
D-glucose
pH 6.5, 37°C, synthetic reaction, cosubstrate alpha-D-mannose 1-phosphate
126
D-glucose
wild-type, pH 6.5, 37°C
34.5
D-glucose
apparent value, pH 6.5, 37°C, synthetic reaction, cosubstrate alpha-D-mannose 1-phosphate
45.1
D-glucose
pH 6.5, 37°C, synthetic reaction, cosubstrate alpha-D-mannose 1-phosphate
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0.269
6-deoxy-6-fluoro-D-glucose
pH 6.5, 37°C, synthetic reaction, cosubstrate alpha-D-mannose 1-phosphate
1.49
6-deoxy-D-glucose
pH 6.5, 37°C, synthetic reaction, cosubstrate alpha-D-mannose 1-phosphate
0.076 - 21.6
alpha-D-mannose 1-phosphate
0.261
1,5-anhydro-D-glucitol
apparent value, pH 6.5, 37°C, synthetic reaction, cosubstrate alpha-D-mannose 1-phosphate
0.117
2-deoxy-2-fluoro-D-glucose
apparent value, pH 6.5, 37°C, synthetic reaction, cosubstrate alpha-D-mannose 1-phosphate
0.129
2-deoxy-D-glucose
apparent value, pH 6.5, 37°C, synthetic reaction, cosubstrate alpha-D-mannose 1-phosphate
0.146
3-deoxy-3-fluoro-D-glucose
apparent value, pH 6.5, 37°C, synthetic reaction, cosubstrate alpha-D-mannose 1-phosphate
0.0976
3-O-methyl-D-glucose
apparent value, pH 6.5, 37°C, synthetic reaction, cosubstrate alpha-D-mannose 1-phosphate
0.91
cellobiose
apparent value, pH 6.5, 37°C, synthetic reaction, cosubstrate alpha-D-mannose 1-phosphate
0.109
D-allose
apparent value, pH 6.5, 37°C, synthetic reaction, cosubstrate alpha-D-mannose 1-phosphate
0.201
D-glucose
apparent value, pH 6.5, 37°C, synthetic reaction, cosubstrate alpha-D-mannose 1-phosphate
0.112
D-mannose
apparent value, pH 6.5, 37°C, synthetic reaction, cosubstrate alpha-D-mannose 1-phosphate
0.429
gentiobiose
apparent value, pH 6.5, 37°C, synthetic reaction, cosubstrate alpha-D-mannose 1-phosphate
0.399
laminaribiose
apparent value, pH 6.5, 37°C, synthetic reaction, cosubstrate alpha-D-mannose 1-phosphate
0.101
maltose
apparent value, pH 6.5, 37°C, synthetic reaction, cosubstrate alpha-D-mannose 1-phosphate
0.236
methyl alpha-D-glucoside
apparent value, pH 6.5, 37°C, synthetic reaction, cosubstrate alpha-D-mannose 1-phosphate
0.413
methyl beta-D-glucoside
apparent value, pH 6.5, 37°C, synthetic reaction, cosubstrate alpha-D-mannose 1-phosphate
0.127
N,N'-diacetylchitobiose
apparent value, pH 6.5, 37°C, synthetic reaction, cosubstrate alpha-D-mannose 1-phosphate
0.148
N-acetyl-D-glucosamine
apparent value, pH 6.5, 37°C, synthetic reaction, cosubstrate alpha-D-mannose 1-phosphate
0.277
sophorose
apparent value, pH 6.5, 37°C, synthetic reaction, cosubstrate alpha-D-mannose 1-phosphate
0.076
alpha-D-mannose 1-phosphate
mutant H245A, pH 6.5, 37°C
21.6
alpha-D-mannose 1-phosphate
wild-type, pH 6.5, 37°C
0.061
D-glucose
mutant H245A, pH 6.5, 37°C
4.86
D-glucose
wild-type, pH 6.5, 37°C
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Kawahara, R.; Saburi, W.; Odaka, R.; Taguchi, H.; Ito, S.; Mori, H.; Matsui, H.
Metabolic mechanism of mannan in a ruminal bacterium, Ruminococcus albus, involving two mannoside phosphorylases and cellobiose 2-epimerase: discovery of a new carbohydrate phosphorylase, beta-1,4-mannooligosaccharide phosphorylase
J. Biol. Chem.
287
42389-42399
2012
Ruminococcus albus (E6UBR9), Ruminococcus albus (E6UIS7), Ruminococcus albus, Ruminococcus albus NE1 (E6UBR9), Ruminococcus albus NE1 (E6UIS7), Ruminococcus albus NE1
brenda
Ye, Y.; Saburi, W.; Odaka, R.; Kato, K.; Sakurai, N.; Komoda, K.; Nishimoto, M.; Kitaoka, M.; Mori, H.; Yao, M.
Structural insights into the difference in substrate recognition of two mannoside phosphorylases from two GH130 subfamilies
FEBS Lett.
590
828-837
2016
Ruminococcus albus (E6UIS7), Ruminococcus albus, Ruminococcus albus DSM 20455 (E6UIS7)
brenda