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Information on EC 2.4.1.281 - 4-O-beta-D-mannosyl-D-glucose phosphorylase and Organism(s) Ruminococcus albus and UniProt Accession E6UBR9

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EC Tree
     2 Transferases
         2.4 Glycosyltransferases
             2.4.1 Hexosyltransferases
                2.4.1.281 4-O-beta-D-mannosyl-D-glucose phosphorylase
IUBMB Comments
This enzyme forms part of a mannan catabolic pathway in the anaerobic bacterium Bacteroides fragilis NCTC 9343.
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This record set is specific for:
Ruminococcus albus
UNIPROT: E6UBR9
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Word Map
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  • 2.4.1.281
  • calcitonin
  • receptor-like
  • adrenomedullin
  • gene-related
  • activity-modifying
  • camp
  • vasodilator
  • amylin
  • endothelial
  • artery
  • neuropeptide
  • receptor-activity-modifying
  • migraine
  • protein-coupled
  • calcitonin-receptor-like
  • cgrp-induced
  • intermedin
  • gpcrs
  • calcitonin-like
  • cgrp-mediated
  • trigeminovascular
  • bibn4096bs
  • trigeminal
  • calcitonin-gene-related
  • receptor-activity
  • hypotensive
  • cgrp-receptors
  • am-induced
  • calcrl
The taxonomic range for the selected organisms is: Ruminococcus albus
The expected taxonomic range for this enzyme is: Bacteria, Archaea
Synonyms
ramp1, ramp2, bf0772, more
SYNONYM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
4-O-beta-D-mannopyranosyl-D-glucopyranose + phosphate = D-glucose + alpha-D-mannose 1-phosphate
show the reaction diagram
sequential Bi Bi mechanism
PATHWAY SOURCE
PATHWAYS
-
-
SYSTEMATIC NAME
IUBMB Comments
4-O-beta-D-mannopyranosyl-D-glucopyranose:phosphate alpha-D-mannosyltransferase
This enzyme forms part of a mannan catabolic pathway in the anaerobic bacterium Bacteroides fragilis NCTC 9343.
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
1,5-anhydro-D-glucitol + alpha-D-mannose 1-phosphate
4-O-beta-D-mannopyranosyl-1,5-anhydro-D-glucitol + phosphate
show the reaction diagram
-
-
-
r
2-deoxy-2-fluoro-D-glucose + alpha-D-mannose 1-phosphate
4-O-beta-D-mannopyranosyl-2-deoxy-2-fluoro-D-glucopyranose + phosphate
show the reaction diagram
-
-
-
r
2-deoxy-D-glucose + alpha-D-mannose 1-phosphate
4-O-beta-D-mannopyranosyl-2-deoxy-D-glucopyranose + phosphate
show the reaction diagram
-
-
-
r
3-deoxy-3-fluoro-D-glucose + alpha-D-mannose 1-phosphate
4-O-beta-D-mannopyranosyl-3-deoxy-3-fluoro-D-glucopyranose + phosphate
show the reaction diagram
-
-
-
r
3-O-methyl-D-glucose + alpha-D-mannose 1-phosphate
4-O-beta-D-mannopyranosyl-3-O-methyl-D-glucopyranose + phosphate
show the reaction diagram
-
-
-
r
4-O-beta-D-mannopyranosyl-D-glucopyranose + phosphate
D-glucose + alpha-D-mannose 1-phosphate
show the reaction diagram
-
-
-
r
cellobiose + alpha-D-mannose 1-phosphate
? + phosphate
show the reaction diagram
-
-
-
r
D-allose + alpha-D-mannose 1-phosphate
4-O-beta-D-mannopyranosyl-D-allose + phosphate
show the reaction diagram
-
-
-
r
D-glucose + alpha-D-mannose 1-phosphate
4-O-beta-D-mannopyranosyl-D-glucopyranose + phosphate
show the reaction diagram
-
-
-
r
D-mannose + alpha-D-mannose 1-phosphate
4-O-beta-D-mannopyranosyl-D-mannopyranose + phosphate
show the reaction diagram
-
-
-
r
gentiobiose + alpha-D-mannose 1-phosphate
? + phosphate
show the reaction diagram
-
-
-
r
laminaribiose + alpha-D-mannose 1-phosphate
? + phosphate
show the reaction diagram
-
-
-
r
maltose + alpha-D-mannose 1-phosphate
? + phosphate
show the reaction diagram
-
-
-
r
methyl alpha-D-glucoside + alpha-D-mannose 1-phosphate
methyl [4-O-beta-D-mannopyranosyl]-alpha-D-glucoside + phosphate
show the reaction diagram
-
-
-
r
methyl beta-D-glucoside + alpha-D-mannose 1-phosphate
methyl [4-O-beta-D-mannopyranosyl]-beta-D-glucopyranoside + phosphate
show the reaction diagram
-
-
-
r
N,N'-diacetylchitobiose + alpha-D-mannose 1-phosphate
? + phosphate
show the reaction diagram
-
-
-
r
N-acetyl-D-glucosamine + alpha-D-mannose 1-phosphate
4-O-beta-D-mannopyranosyl-N-acetyl-D-glucosamine + phosphate
show the reaction diagram
-
-
-
r
sophorose + alpha-D-mannose 1-phosphate
? + phosphate
show the reaction diagram
-
-
-
r
4-O-beta-D-mannopyranosyl-D-glucopyranose + phosphate
D-glucose + alpha-D-mannose 1-phosphate
show the reaction diagram
-
-
-
r
6-deoxy-6-fluoro-D-glucose + alpha-D-mannose 1-phosphate
4-O-beta-D-mannopyranosyl-6-deoxy-6-fluoro-D-glucopyranose + phosphate
show the reaction diagram
-
-
-
?
6-deoxy-D-glucose + alpha-D-mannose 1-phosphate
4-O-beta-D-mannopyranosyl-6-deoxy-D-glucopyranose + phosphate
show the reaction diagram
-
-
-
?
D-glucose + alpha-D-mannose 1-phosphate
4-O-beta-D-mannopyranosyl-D-glucopyranose + phosphate
show the reaction diagram
laminaribiose + alpha-D-mannose 1-phosphate
? + phosphate
show the reaction diagram
-
-
-
?
additional information
?
-
NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
4-O-beta-D-mannopyranosyl-D-glucopyranose + phosphate
D-glucose + alpha-D-mannose 1-phosphate
show the reaction diagram
-
-
-
r
4-O-beta-D-mannopyranosyl-D-glucopyranose + phosphate
D-glucose + alpha-D-mannose 1-phosphate
show the reaction diagram
-
-
-
r
6-deoxy-6-fluoro-D-glucose + alpha-D-mannose 1-phosphate
4-O-beta-D-mannopyranosyl-6-deoxy-6-fluoro-D-glucopyranose + phosphate
show the reaction diagram
-
-
-
?
6-deoxy-D-glucose + alpha-D-mannose 1-phosphate
4-O-beta-D-mannopyranosyl-6-deoxy-D-glucopyranose + phosphate
show the reaction diagram
-
-
-
?
D-glucose + alpha-D-mannose 1-phosphate
4-O-beta-D-mannopyranosyl-D-glucopyranose + phosphate
show the reaction diagram
-
-
-
r
laminaribiose + alpha-D-mannose 1-phosphate
? + phosphate
show the reaction diagram
-
-
-
?
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
alpha-D-mannose 1-phosphate
competitive
alpha-D-mannose 1-phosphate
competitive
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
79.2
1,5-anhydro-D-glucitol
apparent value, pH 6.5, 37°C, synthetic reaction, cosubstrate alpha-D-mannose 1-phosphate
48.7
2-deoxy-2-fluoro-D-glucose
apparent value, pH 6.5, 37°C, synthetic reaction, cosubstrate alpha-D-mannose 1-phosphate
90.7
2-deoxy-D-glucose
apparent value, pH 6.5, 37°C, synthetic reaction, cosubstrate alpha-D-mannose 1-phosphate
6.44
3-deoxy-3-fluoro-D-glucose
apparent value, pH 6.5, 37°C, synthetic reaction, cosubstrate alpha-D-mannose 1-phosphate
7.82
3-O-methyl-D-glucose
apparent value, pH 6.5, 37°C, synthetic reaction, cosubstrate alpha-D-mannose 1-phosphate
27.7
4-O-beta-D-mannopyranosyl-D-glucopyranose
pH 6.5, 37°C, phosphorolytic reaction, cosubstrate phosphate
0.324
alpha-D-mannose 1-phosphate
pH 6.5, 37°C, synthetic reaction, cosubstrate D-glucose
22.2
cellobiose
apparent value, pH 6.5, 37°C, synthetic reaction, cosubstrate alpha-D-mannose 1-phosphate
6.14
D-allose
apparent value, pH 6.5, 37°C, synthetic reaction, cosubstrate alpha-D-mannose 1-phosphate
172
D-glucose
apparent value, pH 6.5, 37°C, synthetic reaction, cosubstrate alpha-D-mannose 1-phosphate
45.6
D-mannose
apparent value, pH 6.5, 37°C, synthetic reaction, cosubstrate alpha-D-mannose 1-phosphate
60.8
gentiobiose
apparent value, pH 6.5, 37°C, synthetic reaction, cosubstrate alpha-D-mannose 1-phosphate
29.1
laminaribiose
apparent value, pH 6.5, 37°C, synthetic reaction, cosubstrate alpha-D-mannose 1-phosphate
50.4
maltose
apparent value, pH 6.5, 37°C, synthetic reaction, cosubstrate alpha-D-mannose 1-phosphate
85.3
methyl alpha-D-glucoside
apparent value, pH 6.5, 37°C, synthetic reaction, cosubstrate alpha-D-mannose 1-phosphate
85.2
methyl beta-D-glucoside
apparent value, pH 6.5, 37°C, synthetic reaction, cosubstrate alpha-D-mannose 1-phosphate
49.9
N,N'-diacetylchitobiose
apparent value, pH 6.5, 37°C, synthetic reaction, cosubstrate alpha-D-mannose 1-phosphate
107
N-acetyl-D-glucosamine
apparent value, pH 6.5, 37°C, synthetic reaction, cosubstrate alpha-D-mannose 1-phosphate
0.444
phosphate
pH 6.5, 37°C, phosphorolytic reaction, cosubstrate 4-O-beta-D-mannopyranosyl-D-glucopyranose
49.8
sophorose
apparent value, pH 6.5, 37°C, synthetic reaction, cosubstrate alpha-D-mannose 1-phosphate
0.304
4-O-beta-D-mannopyranosyl-D-glucopyranose
pH 6.5, 37°C, phosphorolytic reaction, cosubstrate phosphate
156
6-deoxy-6-fluoro-D-glucose
pH 6.5, 37°C, synthetic reaction, cosubstrate alpha-D-mannose 1-phosphate
119
6-deoxy-D-glucose
pH 6.5, 37°C, synthetic reaction, cosubstrate alpha-D-mannose 1-phosphate
2.41 - 36.5
alpha-D-mannose 1-phosphate
25.9 - 319
D-glucose
5.34
phosphate
pH 6.5, 37°C, phosphorolytic reaction, cosubstrate 4-O-beta-D-mannopyranosyl-D-glucopyranose
TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
20.7
1,5-anhydro-D-glucitol
apparent value, pH 6.5, 37°C, synthetic reaction, cosubstrate alpha-D-mannose 1-phosphate
5.71
2-deoxy-2-fluoro-D-glucose
apparent value, pH 6.5, 37°C, synthetic reaction, cosubstrate alpha-D-mannose 1-phosphate
11.7
2-deoxy-D-glucose
apparent value, pH 6.5, 37°C, synthetic reaction, cosubstrate alpha-D-mannose 1-phosphate
0.94
3-deoxy-3-fluoro-D-glucose
apparent value, pH 6.5, 37°C, synthetic reaction, cosubstrate alpha-D-mannose 1-phosphate
0.763
3-O-methyl-D-glucose
apparent value, pH 6.5, 37°C, synthetic reaction, cosubstrate alpha-D-mannose 1-phosphate
17.5
4-O-beta-D-mannopyranosyl-D-glucopyranose
pH 6.5, 37°C, phosphorolytic reaction, cosubstrate phosphate
20.2
cellobiose
apparent value, pH 6.5, 37°C, synthetic reaction, cosubstrate alpha-D-mannose 1-phosphate
0.667
D-allose
apparent value, pH 6.5, 37°C, synthetic reaction, cosubstrate alpha-D-mannose 1-phosphate
34.5 - 45.1
D-glucose
5.12
D-mannose
apparent value, pH 6.5, 37°C, synthetic reaction, cosubstrate alpha-D-mannose 1-phosphate
26.1
gentiobiose
apparent value, pH 6.5, 37°C, synthetic reaction, cosubstrate alpha-D-mannose 1-phosphate
11.6
laminaribiose
apparent value, pH 6.5, 37°C, synthetic reaction, cosubstrate alpha-D-mannose 1-phosphate
5.07
maltose
apparent value, pH 6.5, 37°C, synthetic reaction, cosubstrate alpha-D-mannose 1-phosphate
20.7
methyl alpha-D-glucoside
apparent value, pH 6.5, 37°C, synthetic reaction, cosubstrate alpha-D-mannose 1-phosphate
35.2
methyl beta-D-glucoside
apparent value, pH 6.5, 37°C, synthetic reaction, cosubstrate alpha-D-mannose 1-phosphate
6.34
N,N'-diacetylchitobiose
apparent value, pH 6.5, 37°C, synthetic reaction, cosubstrate alpha-D-mannose 1-phosphate
15.8
N-acetyl-D-glucosamine
apparent value, pH 6.5, 37°C, synthetic reaction, cosubstrate alpha-D-mannose 1-phosphate
13.8
sophorose
apparent value, pH 6.5, 37°C, synthetic reaction, cosubstrate alpha-D-mannose 1-phosphate
83.3
4-O-beta-D-mannopyranosyl-D-glucopyranose
pH 6.5, 37°C, phosphorolytic reaction, cosubstrate phosphate
42
6-deoxy-6-fluoro-D-glucose
pH 6.5, 37°C, synthetic reaction, cosubstrate alpha-D-mannose 1-phosphate
177
6-deoxy-D-glucose
pH 6.5, 37°C, synthetic reaction, cosubstrate alpha-D-mannose 1-phosphate
2.76 - 52.1
alpha-D-mannose 1-phosphate
19.3 - 126
D-glucose
kcat/KM VALUE [1/mMs-1]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.261
1,5-anhydro-D-glucitol
apparent value, pH 6.5, 37°C, synthetic reaction, cosubstrate alpha-D-mannose 1-phosphate
0.117
2-deoxy-2-fluoro-D-glucose
apparent value, pH 6.5, 37°C, synthetic reaction, cosubstrate alpha-D-mannose 1-phosphate
0.129
2-deoxy-D-glucose
apparent value, pH 6.5, 37°C, synthetic reaction, cosubstrate alpha-D-mannose 1-phosphate
0.146
3-deoxy-3-fluoro-D-glucose
apparent value, pH 6.5, 37°C, synthetic reaction, cosubstrate alpha-D-mannose 1-phosphate
0.0976
3-O-methyl-D-glucose
apparent value, pH 6.5, 37°C, synthetic reaction, cosubstrate alpha-D-mannose 1-phosphate
0.91
cellobiose
apparent value, pH 6.5, 37°C, synthetic reaction, cosubstrate alpha-D-mannose 1-phosphate
0.109
D-allose
apparent value, pH 6.5, 37°C, synthetic reaction, cosubstrate alpha-D-mannose 1-phosphate
0.201
D-glucose
apparent value, pH 6.5, 37°C, synthetic reaction, cosubstrate alpha-D-mannose 1-phosphate
0.112
D-mannose
apparent value, pH 6.5, 37°C, synthetic reaction, cosubstrate alpha-D-mannose 1-phosphate
0.429
gentiobiose
apparent value, pH 6.5, 37°C, synthetic reaction, cosubstrate alpha-D-mannose 1-phosphate
0.399
laminaribiose
apparent value, pH 6.5, 37°C, synthetic reaction, cosubstrate alpha-D-mannose 1-phosphate
0.101
maltose
apparent value, pH 6.5, 37°C, synthetic reaction, cosubstrate alpha-D-mannose 1-phosphate
0.236
methyl alpha-D-glucoside
apparent value, pH 6.5, 37°C, synthetic reaction, cosubstrate alpha-D-mannose 1-phosphate
0.413
methyl beta-D-glucoside
apparent value, pH 6.5, 37°C, synthetic reaction, cosubstrate alpha-D-mannose 1-phosphate
0.127
N,N'-diacetylchitobiose
apparent value, pH 6.5, 37°C, synthetic reaction, cosubstrate alpha-D-mannose 1-phosphate
0.148
N-acetyl-D-glucosamine
apparent value, pH 6.5, 37°C, synthetic reaction, cosubstrate alpha-D-mannose 1-phosphate
0.277
sophorose
apparent value, pH 6.5, 37°C, synthetic reaction, cosubstrate alpha-D-mannose 1-phosphate
0.269
6-deoxy-6-fluoro-D-glucose
pH 6.5, 37°C, synthetic reaction, cosubstrate alpha-D-mannose 1-phosphate
1.49
6-deoxy-D-glucose
pH 6.5, 37°C, synthetic reaction, cosubstrate alpha-D-mannose 1-phosphate
0.076 - 21.6
alpha-D-mannose 1-phosphate
0.061 - 4.86
D-glucose
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
-
UniProt
Manually annotated by BRENDA team
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
209000
gel filtration
37823
6 * 38000, SDS-PAGE, 4 * 37823, amino acid sequence
38000
6 * 38000, SDS-PAGE, 4 * 37823, amino acid sequence
43646
2 * 45000, SDS-PAGE, 4 * 43646, amino acid sequence
45000
2 * 45000, SDS-PAGE, 4 * 43646, amino acid sequence
80000
gel filtration
SUBUNIT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
homohexamer
6 * 38000, SDS-PAGE, 4 * 37823, amino acid sequence
homodimer
2 * 45000, SDS-PAGE, 4 * 43646, amino acid sequence
CRYSTALLIZATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
crystal structure with/without 4-O-beta-D-mannosyl-D-glucose. His245 of loop 3 forms a hydrogen-bond network with the substrate through a water molecule, and is indispensible for substrate binding
PROTEIN VARIANTS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
H245A
1.47% of wild-type phosphorolytic activity
pH STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
3.5 - 9.5
4°C, 24 h stable
725502
4.5 - 10.5
4°C, 24 h stable
725502
TEMPERATURE STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
expressed in Escherichia coli BL21
expressed in Escherichia coli BL21
REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Kawahara, R.; Saburi, W.; Odaka, R.; Taguchi, H.; Ito, S.; Mori, H.; Matsui, H.
Metabolic mechanism of mannan in a ruminal bacterium, Ruminococcus albus, involving two mannoside phosphorylases and cellobiose 2-epimerase: discovery of a new carbohydrate phosphorylase, beta-1,4-mannooligosaccharide phosphorylase
J. Biol. Chem.
287
42389-42399
2012
Ruminococcus albus (E6UBR9), Ruminococcus albus (E6UIS7), Ruminococcus albus, Ruminococcus albus NE1 (E6UBR9), Ruminococcus albus NE1 (E6UIS7), Ruminococcus albus NE1
Manually annotated by BRENDA team
Ye, Y.; Saburi, W.; Odaka, R.; Kato, K.; Sakurai, N.; Komoda, K.; Nishimoto, M.; Kitaoka, M.; Mori, H.; Yao, M.
Structural insights into the difference in substrate recognition of two mannoside phosphorylases from two GH130 subfamilies
FEBS Lett.
590
828-837
2016
Ruminococcus albus (E6UIS7), Ruminococcus albus, Ruminococcus albus DSM 20455 (E6UIS7)
Manually annotated by BRENDA team