Information on EC 2.4.1.28 - glucosyl-DNA beta-glucosyltransferase

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The expected taxonomic range for this enzyme is: Enterobacteria phage T6

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EC NUMBER
COMMENTARY hide
2.4.1.28
-
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RECOMMENDED NAME
GeneOntology No.
glucosyl-DNA beta-glucosyltransferase
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REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
transfers a beta-D-glucosyl residue from UDP-glucose to a glucosylhydroxymethylcytosine residue in DNA
show the reaction diagram
-
-
-
-
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REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
hexosyl group transfer
-
-
-
-
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SYSTEMATIC NAME
IUBMB Comments
UDP-glucose:D-glucosyl-DNA beta-D-glucosyltransferase
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CAS REGISTRY NUMBER
COMMENTARY hide
9030-15-3
-
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ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
Escherichia coli infected with
-
-
Manually annotated by BRENDA team
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SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
UDP-glucose + glucosyl-5-hydroxymethylcytosine residue in DNA
UDP + beta-D-glucosyl-beta-D-glucosyl-5-hydroxymethylcytosine containing DNA
show the reaction diagram
-
no transfer of glucose to synthetic 5-hydroxymethylcytosine-DNA or to T6 DNA, enzyme glucosylates glucosylhydroxymethylcytosine residues of either alpha or beta configuration
-
?
UDP-glucose + glucosylhydroxymethylcytosine residue in DNA
UDP + beta-D-glucosyl-beta-D-glucosyl-hydroxymethylcytosine containing DNA
show the reaction diagram
-
-
-
?
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NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
UDP-glucose + glucosylhydroxymethylcytosine residue in DNA
UDP + beta-D-glucosyl-beta-D-glucosyl-hydroxymethylcytosine containing DNA
show the reaction diagram
-
-
-
?
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METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Mg2+
-
required for activity
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ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
sulfhydryl reagents
-
required
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SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
0.0017
-
crude extract
0.0067
-
purified enzyme
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pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
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TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
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Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
partial, using streptomycin sulfate treatment and fractionation on DEAE-cellulose
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LINKS TO OTHER DATABASES (specific for EC-Number 2.4.1.28)
ExplorEnz
ExPASy
KEGG
MetaCyc
SABIO-RK
NCBI: PubMed, Protein, Nucleotide, Structure, Genome, OMIM
IUBMB Enzyme Nomenclature
PROSITE Database of protein families and domains
SYSTERS
Protein Mutant Database
InterPro (database of protein families, domains and functional sites)