Information on EC 2.4.1.274 - glucosylceramide beta-1,4-galactosyltransferase

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The expected taxonomic range for this enzyme is: Euarchontoglires

SplaateEC_Number,Commentary
EC NUMBER
COMMENTARY
2.4.1.274
-
SplaateRecommended_Name,GO_Number
RECOMMENDED NAME
GeneOntology No.
glucosylceramide beta-1,4-galactosyltransferase
-
SplaateReaction,Reaction_id,Commentary,IF(Commentary != '',Organism,'') ,IF(Commentary != '',Literature,'')
REACTION
REACTION DIAGRAM
COMMENTARY
ORGANISM
UNIPROT
LITERATURE
UDP-alpha-D-galactose + beta-D-glucosyl-(1<->1)-ceramide = UDP + beta-D-galactosyl-(1->4)-beta-D-glucosyl-(1<->1)-ceramide
show the reaction diagram
-
-
-
-
SplaateReaction_Type,Organism,Commentary,Literature
SplaatePathway,BRENDA_Link,KEGG_Link,MetaCyc_Link,Source_Database
PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
Sphingolipid metabolism
-
-
Metabolic pathways
-
-
SplaateSystematic_Name,Commentary_IUBMB
SYSTEMATIC NAME
IUBMB Comments
UDP-galactose:beta-D-glucosyl-(1<->1)-ceramide beta-1,4-galactosyltransferase
Involved in the synthesis of several different major classes of glycosphingolipids.
SplaateSynonyms,Organism,Commentary,Literature
SYNONYMS
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
LacCer synthase
-
LacCer synthase
-
-
lactosylceramide synthase
-
lactosylceramide synthase
-
-
UDP-Ga1:glucosylceramide beta1->4galactosyltransferase
-
-
UDP-Gal:glucosylceramide beta-1,4-galactosyltransferase
-
-
UDP-galactose:GlcCer, beta1-4 galactosyltransferase
-
-
UDPgalactose:glucosylceramide beta1->4-galactosyltransferase
-
-
SplaateCAS_Registry_Number,Commentary
SplaateOrganism, Commentary,Literature, Sequence_Code,Sequence_db,Textmining
ORGANISM
COMMENTARY
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
-
SwissProt
Manually annotated by BRENDA team
SplaateGeneral_Information, Organism, Commentary, Literature
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
physiological function
-
elevated glucosylceramide beta-1,4-galactosyltransferase activity may contribute to the storage of lactosylceramide urinary proximal tubular cells from familial hypercholesterolemic patients
SplaateSubstrates,Products,id,Organism_Substrates,Commentary_Substrates, Literature_Substrates, Commentary_Products, Literature_Products,Reversibility
SUBSTRATE
PRODUCT                      
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
LITERATURE
(Substrate)
COMMENTARY
(Product)
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
UDP-galactose + beta-D-glucosyl-(1<->1)-ceramide
UDP + beta-D-galactosyl-(1->4)-beta-D-glucosyl-(1<->1)-ceramide
show the reaction diagram
-
-
-
?
UDP-galactose + beta-D-glucosyl-(1<->1)-ceramide
UDP + beta-D-galactosyl-(1->4)-beta-D-glucosyl-(1<->1)-ceramide
show the reaction diagram
-
-
-
?
UDP-galactose + beta-D-glucosyl-(1<->1)-ceramide
UDP + beta-D-galactosyl-(1->4)-beta-D-glucosyl-(1<->1)-ceramide
show the reaction diagram
-
-
-
?
UDP-galactose + beta-D-glucosyl-(1<->1)-ceramide
UDP + beta-D-galactosyl-(1->4)-beta-D-glucosyl-(1<->1)-ceramide
show the reaction diagram
-
-
-
?
UDP-galactose + beta-D-glucosyl-(1<->1)-ceramide
UDP + beta-D-galactosyl-(1->4)-beta-D-glucosyl-(1<->1)-ceramide
show the reaction diagram
-
the enzyme is involved in the synthesis of several different major classes of glycosphingolipids
-
?
UDP-galactose + ceramide
?
show the reaction diagram
-
-
-
-
?
UDP-galactose + galactosylceramide
?
show the reaction diagram
-
-
-
-
?
UDP-galactose + GlcNAc-beta-(1->3)-Gal-beta-(1->4)-Glc-beta-(1<->1)-Cer
?
show the reaction diagram
-
-
-
-
?
UDP-galactose + lactosylceramide
?
show the reaction diagram
-
-
-
-
?
SplaateNatural_Substrates,Natural_Products,id,Organism_Substrates,Commentary_Substrates,Literature_Substrates,Commentary_Products,Literature_Products,Reversibility
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
LITERATURE
(Substrate)
COMMENTARY
(Product)
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
UDP-galactose + beta-D-glucosyl-(1<->1)-ceramide
UDP + beta-D-galactosyl-(1->4)-beta-D-glucosyl-(1<->1)-ceramide
show the reaction diagram
-
-
-
?
UDP-galactose + beta-D-glucosyl-(1<->1)-ceramide
UDP + beta-D-galactosyl-(1->4)-beta-D-glucosyl-(1<->1)-ceramide
show the reaction diagram
-
-
-
?
UDP-galactose + beta-D-glucosyl-(1<->1)-ceramide
UDP + beta-D-galactosyl-(1->4)-beta-D-glucosyl-(1<->1)-ceramide
show the reaction diagram
-
the enzyme is involved in the synthesis of several different major classes of glycosphingolipids
-
?
SplaateCofactor,Organism,Commentary,Literature,Filename
SplaateMetals_Ions,Organism,Commentary, Literature
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
Ca2+
-
the enzyme is activated by Mn2+ and to a lesser extent by Mg2+ and Ca2+
Mg2+
-
the enzyme is inactive without added metal ions. Maximal activity is obtained in the presence of 1.0 mM Mn2+/Mg2+ (1:1)
Mg2+
-
the enzyme is activated by Mn2+ and to a lesser extent by Mg2+ and Ca2+
Mn2+
-
required
Mn2+
-
optimum activity is obtained in the presence of 1.0 mM Mn2+/Mg2+ (1:1)
Mn2+
-
the enzyme is activated by Mn2+ and to a lesser extent by Mg2+ and Ca2+
SplaateInhibitors, Organism, Commentary, Literature,Filename
SplaateActivating_Compound, Organism, Commentary, Literature,Filename
ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
Cutscum
-
maximal activity in a detergent mixture, Triton X-100/Cutscum (1:2, v/v), 0.1 mg/ml
-
Triton X-100
-
maximal activity in a detergent mixture, Triton X-100/Cutscum (1:2, v/v), 0.1 mg/ml
SplaateKM_Value,KM_Value_Maximum, Substrate,Organism, Commentary, Literature, Filename
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
0.003
beta-D-glucosyl-(1<->1)-ceramide
-
pH 6.8, 37C
0.0237
beta-D-glucosyl-(1<->1)-ceramide
-
pH 7.4, 37C
0.11
beta-D-glucosyl-(1<->1)-ceramide
-
pH 7.0, 37C
0.0005
UDP-galactose
-
pH 6.8, 37C
0.0806
UDP-galactose
-
pH 7.4, 37C
0.52
UDP-galactose
-
pH 7.0, 37C
SplaateTurnover_Number, Turnover_Number_Maximum, Substrate,Organism,Commentary, Literature, Filename
SplaateKCat_KM_Value,KCat_KM_Value_Maximum, Substrate,Organism, Commentary, Literature, Filename
SplaateKI_Value,KI_Value_Maximum, Inhibitor,Organism, Commentary, Literature, Filename
SplaateIC50_Value,IC50_Value_Maximum, Inhibitor,Organism, Commentary, Literature, Filename
SplaateSpecific_Activity, Specific_Activity_Maximum, Organism ,Commentary, Literature
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
0.0613
recombinant enzyme, pH and temperature not specified in the publication
SplaatepH_Optimum, pH_Optimum_Maximum, Organism, Commentary, Literature
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
6.8
-
assay at
7.2
-
cacodylate buffer
7.4
-
assay at
SplaatepH_Range,pH_Range_Maximum, Organism,Commentary, Literature
SplaateTemperature_Optimum, Temperature_Optimum_Maximum, Organism, Commentary, Literature
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
37
-
assay at
37
-
assay at
37
-
assay at
SplaateTemperature_Range, Temperature_Range_Maximum, Organism, Commentary, Literature
SplaatepI_Value,pI_Value_Maximum, Organism,Commentary, Literature
pI VALUE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
4.55
-
isoelectric focusing
SplaateSource_Tissue, Organism, Commentary, Literature, Textmining
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
SOURCE
high expression
Manually annotated by BRENDA team
-
high activity
Manually annotated by BRENDA team
low axpression
Manually annotated by BRENDA team
low axpression
Manually annotated by BRENDA team
-
low activity
Manually annotated by BRENDA team
low axpression
Manually annotated by BRENDA team
-
from normal and familial hypercholesterolemic homozygotes. Urinary proximal tubular cells from familial hypercholesterolemic homozygous patients contain glucosylceramide beta-1,4-galactosyltransferase activity 35% higher as compared to control cells
Manually annotated by BRENDA team
SplaateLocalization, Organism, Commentary, id_go, Literature, Textmining
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY
GeneOntology No.
LITERATURE
SOURCE
SplaatePDB,PDB,PDB,Organism,Uniprot_ID
SplaateMolecular_Weight, Molecular_Weight_Maximum, Organism, Commentary, Literature
SplaateSubunits, Organism, Commentary, Literature
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
?
-
x * 44776, calculated from sequence; x * 61000, SDS-PAGE
SplaatePosttranslational_Modification, Organism, Commentary, Literature
POSTTRANSLATIONAL MODIFICATION
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
glycoprotein
-
contains about 15% N-linked carbohydrate. The polypeptide contains eight putative glycosylation sites
SplaateCommentary, Organism, Literature
SplaatepH_Stability,pH_Stability_Maximum, Organism, Commentary, Literature
SplaateTemperature_Stability,Temperature_Stability_Maximum, Organism, Commentary, Literature
SplaateGeneral_Stability, Organism, Literature
SplaateOrganic_Solvent, Organism, Commentary, Literature
SplaateOxidation_Stability,Organism,Literature
SplaateStorage_Stability, Organism, Literature
SplaateCommentary, Organism, Literature
Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
SplaateCommentary, Organism, Literature
Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
expression in Sf9 cells
expression in Sf9 cells
-
SplaateCommentary, Organism, Literature
SplaateEngineering, Organism, Commentary, Literature
SplaateCommentary, Organism, Literature
SplaateApplication,Organism,Commentary,Literature