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Information on EC 2.4.1.273 - soyasaponin III rhamnosyltransferase and Organism(s) Glycine max and UniProt Accession D4Q9Z5

for references in articles please use BRENDA:EC2.4.1.273
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EC Tree
     2 Transferases
         2.4 Glycosyltransferases
             2.4.1 Hexosyltransferases
                2.4.1.273 soyasaponin III rhamnosyltransferase
IUBMB Comments
Part of the biosynthetic pathway for soyasaponins.
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This record set is specific for:
Glycine max
UNIPROT: D4Q9Z5
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The taxonomic range for the selected organisms is: Glycine max
The enzyme appears in selected viruses and cellular organisms
Synonyms
gmsgt3, more
SYNONYM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
GmSGT3
-
-
-
-
UGT91H4
PATHWAY SOURCE
PATHWAYS
-
-
SYSTEMATIC NAME
IUBMB Comments
UDP-rhamnose:soyasaponin III rhamnosyltransferase
Part of the biosynthetic pathway for soyasaponins.
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
UDP-rhamnose + soyasaponin III
UDP + soyasaponin I
show the reaction diagram
UDP-beta-L-rhamnose + soyasaponin III
UDP + soyasaponin I
show the reaction diagram
-
-
-
?
NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
UDP-rhamnose + soyasaponin III
UDP + soyasaponin I
show the reaction diagram
the enzyme is involved in the biosynthesis of soyasaponin I in Glycine max
-
-
?
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
7.4
assay at
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
37
assay at
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
-
SwissProt
Manually annotated by BRENDA team
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
physiological function
part of the biosynthetic pathway for soyasaponins
physiological function
a double mutant lacking UGT91H9 and UGT91H4 fails to accumulate soyasaponins Ac and betag, and also soyasaponins Ab and alphag, i.e. the third Glc and Rha conjugations are both disabled
UNIPROT
ENTRY NAME
ORGANISM
NO. OF AA
NO. OF TRANSM. HELICES
MOLECULAR WEIGHT[Da]
SOURCE
SEQUENCE
LOCALIZATION PREDICTION?
SGT3_SOYBN
472
0
53518
Swiss-Prot
other Location (Reliability: 2)
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
53518
x * 53518, calculated from sequence
SUBUNIT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
?
x * 53518, calculated from sequence
PROTEIN VARIANTS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
R339H
mutant fails to accumulate soyasaponins Ac and betag
CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
expression in Escherichia coli
REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Shibuya, M.; Nishimura, K.; Yasuyama, N.; Ebizuka, Y.
Identification and characterization of glycosyltransferases involved in the biosynthesis of soyasaponin I in Glycine max
FEBS Lett.
584
2258-2264
2010
Glycine max (D4Q9Z5), Glycine max
Manually annotated by BRENDA team
Yano, R.; Takagi, K.; Tochigi, S.; Fujisawa, Y.; Nomura, Y.; Tsuchinaga, H.; Takahashi, Y.; Takada, Y.; Kaga, A.; Anai, T.; Tsukamoto, C.; Seki, H.; Muranaka, T.; Ishimoto, M.
Isolation and characterization of the soybean Sg-3 gene that is involved in genetic variation in sugar chain composition at the C-3 position in soyasaponins
Plant Cell Physiol.
59
792-805
2018
Glycine max (A0A0R0INR5), Glycine max
Manually annotated by BRENDA team