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Information on EC 2.4.1.253 - baicalein 7-O-glucuronosyltransferase and Organism(s) Scutellaria baicalensis and UniProt Accession Q76MR7
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2.4.1.253 baicalein 7-O-glucuronosyltransferaseIUBMB Comments
The enzyme is specific for UDP-D-glucuronate as a sugar donor and flavones with substitution ortho- to the 7-OH group such as baicalein (6-OH), scutellarein (6-OH) and wogonin (8-OMe).
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The taxonomic range for the selected organisms is: Scutellaria baicalensis
The expected taxonomic range for this enzyme is: Scutellaria baicalensis
The expected taxonomic range for this enzyme is: Scutellaria baicalensis
Synonyms
ubgat, udp-glucuronate baicalein 7-o-glucuronosyltransferase, baicalein 7-o-glucuronosyltransferase, udp-glucuronate:baicalein 7-o-glucuronosyltransferase, 
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SYNONYM 
ORGANISM
UNIPROT
COMMENTARY 
LITERATURE
PATHWAY SOURCE
PATHWAYS
-
-, -
SYSTEMATIC NAME
IUBMB Comments
UDP-D-glucuronate:5,6,7-trihydroxyflavone 7-O-glucuronosyltransferase
The enzyme is specific for UDP-D-glucuronate as a sugar donor and flavones with substitution ortho- to the 7-OH group such as baicalein (6-OH), scutellarein (6-OH) and wogonin (8-OMe).
SUBSTRATE
PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
(Substrate)
LITERATURE
(Substrate)
(Substrate)
COMMENTARY
(Product)
(Product)
LITERATURE
(Product)
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
UDP-D-glucuronate + 4',5,6,7-tetrahydroxyflavone
UDP + 4',5,6,7-tetrahydroxyflavone 7-O-beta-D-glucuronate
-
i.e. 5,6,7-trihydroxy-2-(4-hydroxyphenyl)chromen-4-one, i.e. scutellarein. The enzyme is specific to UDP-glucuronic acid as a sugar donor and flavones with substitution ortho- to the 7-OH group such as baicalein (6-OH), scutellarein (6-OH) and wogonin (8-OMe)
i.e. scutellarein 7-O-glucuronide
-
?
UDP-D-glucuronate + 5,6,7-trihydroxyflavone
UDP + 5,6,7-trihydroxyflavone-7-O-beta-D-glucuronate
-
i.e. 5,6,7-trihydroxy-2-phenyl-4H-chromen-4-one, i.e. baicalein. The enzyme is specific to UDP-glucuronic acid as a sugar donor and flavones with substitution ortho- to the 7-OH group such as baicalein (6-OH), scutellarein (6-OH) and wogonin (8-OMe)
i.e. 5,6-dihydroxy-4-oxo-2-phenyl-4H-chromen-7-yl beta-D-glucopyranosiduronic acid, i.e. baicalein 7-O-glucuronide, i.e. 5,6-dihydroxy-4-oxo-2-phenyl-4H-chromen-7-yl beta-D-glucopyranosiduronic acid, i.e. baicalein 7-O-glucuronide. Identification and quantitation of the enzyme reaction products are carried out by reversed phase-HPLC
-
?
UDP-D-glucuronate + 5,7-dihydroxy-8-methoxyflavone
UDP + 5,7-dihydroxy-8-methoxyflavone-7-O-beta-D-glucuronate
-
i.e. 5,7-dihydroxy-8-methoxy-2-phenylchromen-4-one, i.e. wogonin. The enzyme is specific to UDP-glucuronic acid as a sugar donor and flavones with substitution ortho- to the 7-OH group such as baicalein (6-OH), scutellarein (6-OH) and wogonin (8-OMe)
i.e wogonin 7-O-glucuronide
-
?
additional information
?
-
-
flavonoids lacking a substitution at the position ortho- to the 7-OH (chrysin, apigenin, luteolin, quercetin, formononetin and daidzein) do not serve as substrates
-
-
?
NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
(Substrate)
LITERATURE
(Substrate)
(Substrate)
COMMENTARY
(Product)
(Product)
LITERATURE
(Product)
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
UDP-D-glucuronate + 5,6,7-trihydroxyflavone
UDP + 5,6,7-trihydroxyflavone-7-O-beta-D-glucuronate
-
i.e. 5,6,7-trihydroxy-2-phenyl-4H-chromen-4-one, i.e. baicalein. The enzyme is specific to UDP-glucuronic acid as a sugar donor and flavones with substitution ortho- to the 7-OH group such as baicalein (6-OH), scutellarein (6-OH) and wogonin (8-OMe)
i.e. 5,6-dihydroxy-4-oxo-2-phenyl-4H-chromen-7-yl beta-D-glucopyranosiduronic acid, i.e. baicalein 7-O-glucuronide
-
?
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
4,4'-diisothiocyanostilbene-2,2'-disulfonic acid
-
0.1 mM, complete inhibition
additional information
-
N-ethylmaleimide has no effect in activity
-
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
kcat/KM VALUE [1/mMs-1]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
additional information
additional information
-
Vmax/Km ratio for 5,6,7-trihydroxy-2-phenyl-4H-chromen-4-one (i.e. baicalein) is: 4.9 nkat/mg*micromol, Vmax/Km ratio for scutellarein is 2.67 nkat/mg*micromol, Vmax/Km ratio for wogonin is: 1.71 nkat/mg*micromol
-
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
pI VALUE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
ORGANISM
COMMENTARY
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
SOURCE
-
callus tissue derived from the petiole of a sterile young plant
UNIPROT
ENTRY NAME
ORGANISM
NO. OF AA
NO. OF TRANSM. HELICES
MOLECULAR WEIGHT[Da]
SOURCE
SEQUENCE
LOCALIZATION PREDICTION
The reliability class of the localization prediction ranges from 1 (very reliable) to 5 (not reliable).
The reliability class of the localization prediction ranges from 1 (very reliable) to 5 (not reliable). UBGAT_SCUBA
441
0
48654
Swiss-Prot
Secretory Pathway (Reliability: 4)
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
SUBUNIT
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
homodimer
-
2 * 52000, does not exist or function as monomer, SDS-PAGE
TEMPERATURE STABILITY
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
23
-
50% of the activity remains after 3 h, 25% of the activity remains after 24 h
STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
EXPRESSION
ORGANISM
UNIPROT
LITERATURE
compared to the control, at the 3 day, 0.000002 mM/l and 0.0002 mM/l H2O2 promotes the expression of UDP-glucuronate baicalein 7-O-glucuronosyltransferase
high temperature of 40°C increases the level of UDP-glucuronate:baicalein 7-O-glucuronosyltransferase transcripts, whereas low temperature does not affect the level of these transcripts compared with their level at 30°C. At 40°C, the expression after 40-days exposure is significantly higher than that after 30 days
REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Nagashima, S.; Hirotani, M.; Yoshikawa, T.
Purification and characterization of UDP-glucuronate: baicalein 7-O-glucuronosyltransferase from Scutellaria baicalensis Georgi. cell suspension cultures
Phytochemistry
53
533-538
2000
Scutellaria baicalensis, Scutellaria baicalensis Georgi
Wang, W.; Chen, J.-k.; Zhou, T.-s.
CHS and UBGAT expression and baicalin accumulation in the roots of Scutellaria baicalensis Georgi during cultivation seasons
Fudan Xuebao Ziran Kexueban
45
674-678
2006
Scutellaria baicalensis
-
Yuan, Y.; Shuai, L.; Chen, S.; Huang, L.; Qin, S.; Yang, Z.
Flavonoids and antioxidative enzymes in temperature-challenged roots of Scutellaria baicalensis Georgi
Z. Naturforsch. C
67
77-85
2012
Scutellaria baicalensis (Q76MR7), Scutellaria baicalensis
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