Information on EC 2.4.1.232 - initiation-specific alpha-1,6-mannosyltransferase

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The expected taxonomic range for this enzyme is: Eukaryota

EC NUMBER
COMMENTARY hide
2.4.1.232
-
RECOMMENDED NAME
GeneOntology No.
initiation-specific alpha-1,6-mannosyltransferase
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
Transfers an alpha-D-mannosyl residue from GDP-mannose into lipid-linked oligosaccharide, forming an alpha-(1->6)-D-mannosyl-D-mannose linkage
show the reaction diagram
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
glycosyl group transfer
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hexosyl group transfer
PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
Metabolic pathways
-
-
Various types of N-glycan biosynthesis
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-
SYSTEMATIC NAME
IUBMB Comments
GDP-mannose:oligosaccharide 6-alpha-D-mannosyltransferase
Requires Mn2+. In Saccharomyces cerevisiae, this enzyme catalyses an essential step in the outer chain elongation of N-linked oligosaccharides. Man8GlcNAc and Man9GlcNAc are equally good substrates.
CAS REGISTRY NUMBER
COMMENTARY hide
135622-87-6
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346003-17-6
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346003-17-6
Schizosaccharomyces pombe gene och1+
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
strain J-1012 and B-792
-
-
Manually annotated by BRENDA team
Candida albicans NIH B-792 serotype B
NIH B-792 serotype B strain
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-
Manually annotated by BRENDA team
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SwissProt
Manually annotated by BRENDA team
Och1p
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-
Manually annotated by BRENDA team
gene ALG12
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-
Manually annotated by BRENDA team
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
malfunction
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the variant surface glycoprotein sVSG221 synthesized by the ALG12-/- parasites shows different glycosylation patterns to that synthesized by wild-type cells, changes in sVSG221 glycosylation induced by the deletion of the ALG12 gene, overview
physiological function
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the TbALG12 gene encodes the alpha1-6-mannosyltransferase that converts Man7GlcNAc2-PP-Dol to Man8GlcNAc2-PP-Dol
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
alpha-D-mannose + Manalpha(1,6)[Manalpha(1,3)]Manalpha(1,6)[Manalpha(1,2)Manalpha(1,2)Manalpha(1,3)]Manbeta(1,4)GlcNAcbeta(1,4)GlcNAc-pyridylamine
?
show the reaction diagram
-
recombinant enzyme shows 28.8% of the activity with Manalpha(1,2)Manalpha(1,6)[Manalpha(1,3)]Manalpha(1,6)[Manalpha(1,2)Manalpha(1,2)Manalpha(1,3)]Manbeta(1,4)GLcNAcbeta(1,4)GlcNAc-pyridylamine
-
-
?
GDP-mannose + alpha-1,6-D-mannobiose
?
show the reaction diagram
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two distinct Mnn9p-containing multi-protein complexes add multiple mannosyl residues forming a series of poly-mannose structures in vitro, no substrate of Och1p
formation of a series of poly-mannose structures, which contain mainly alpha-1,6- and some additional alpha-1,2-linked mannoses
?
GDP-mannose + Man8GlcNAc
GDP + Man9GlcNAc
show the reaction diagram
-
Man8GlcNAc from thyroglobulin Man9GlcNAc by treatment with yeast specific mannosidase, specificity, enzyme catalyzes addition of mannose to the alpha-1,3-mannose of the substrate, initiates outer chain formation
-
?
GDP-mannose + Man8GlcNAc2-PA
GDP + Man9GlcNAc2-PA
show the reaction diagram
GDP-mannose + Man9GlcNAc
GDP + Man10GlcNAc
show the reaction diagram
-
Man9GlcNAc from thyroglobulin, specificity, enzyme catalyzes addition of mannose to the alpha-1,3-mannose of the substrate, initiates outer chain formation
-
?
GDP-mannose + Man9GlcNAc2-PA
GDP + Man10GlcNAc2-PA
show the reaction diagram
GDP-mannose + Manalpha(1,2)Manalpha(1,6)[Manalpha(1,2)Manalpha(1,3)]Manalpha(1,6)[Manalpha(1,2)Manalpha(1,2)Manalpha(1,3)]Manbeta(1,4)GlcNAcbeta(1,4)GlcNAc-pyridylamine
?
show the reaction diagram
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recombinant enzyme shows 74.2% of the activity with Manalpha(1,2)Manalpha(1,6)[Manalpha(1,3)]Manalpha(1,6)[Manalpha(1,2)Manalpha(1,2)Manalpha(1,3)]Manbeta(1,4)GLcNAcbeta(1,4)GlcNAc-pyridylamine. Och1p has the catalytic potential to transfer two molecules of mannose to Manalpha(1,2)Manalpha(1,6)[Manalpha(1,2)Manalpha(1,3)]Manalpha(1,6)[Manalpha(1,2)Manalpha(1,2)Manalpha(1,3)]Manbeta(1,4)GlcNAcbeta(1,4)GlcNAc-pyridylamine. The second mannose is attached with alpha1,6-linkage to either the alpha1,6-linked or the alpha1,3-linked mannose that is attached to alpha1,6-linked mannose
-
-
?
GDP-mannose + Manalpha(1,2)Manalpha(1,6)[Manalpha(1,2)Manalpha(1,3)]Manalpha(1,6)[Manalpha(1,2)Manalpha(1,3)]Manbeta(1,4)GlcNAcbeta(1,4)GlcNAc-pyridylamine
?
show the reaction diagram
-
recombinant enzyme shows 58.5% of the activity with Manalpha(1,2)Manalpha(1,6)[Manalpha(1,3)]Manalpha(1,6)[Manalpha(1,2)Manalpha(1,2)Manalpha(1,3)]Manbeta(1,4)GLcNAcbeta(1,4)GlcNAc-pyridylamine
-
-
?
GDP-mannose + Manalpha(1,2)Manalpha(1,6)[Manalpha(1,3)]Manalpha(1,6)[Manalpha(1,2)Manalpha(1,2)Manalpha(1,3)]Manbeta(1,4)GlcNAcbeta(1,4)GlcNAc-pyridylamine
?
show the reaction diagram
-
-
-
-
?
GDP-mannose + Manalpha(1,2)Manalpha(1,6)[Manalpha(1,3)]Manalpha(1,6)[Manalpha(1,2)Manalpha(1,3)]Manbeta(1,4)GlcNAcbeta(1,4)GlcNAc-pyridylamine
?
show the reaction diagram
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recombinant enzyme shows 66.1% of the activity with Manalpha(1,2)Manalpha(1,6)[Manalpha(1,3)]Manalpha(1,6)[Manalpha(1,2)Manalpha(1,2)Manalpha(1,3)]Manbeta(1,4)GLcNAcbeta(1,4)GlcNAc-pyridylamine
-
-
?
GDP-mannose + Manalpha(1,6)[Manalpha(1,2)Manalpha(1,3)]Manalpha(1,6)[Manalpha(1,2)Manalpha(1,2)Manalpha(1,3)]Manbeta(1,4)GlcNAcbeta(1,4)GlcNAc-pyridylamine
?
show the reaction diagram
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recombinant enzyme shows 28.8% of the activity with Manalpha(1,2)Manalpha(1,6)[Manalpha(1,3)]Manalpha(1,6)[Manalpha(1,2)Manalpha(1,2)Manalpha(1,3)]Manbeta(1,4)GLcNAcbeta(1,4)GlcNAc-pyridylamine
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-
?
GDP-mannose + Manalpha(1,6)[Manalpha(1,2)Manalpha(1,3)]Manalpha(1,6)[Manalpha(1,2)Manalpha(1,3)]Manbeta(1,4)GlcNAcbeta(1,4)GlcNAc-pyridylamine
?
show the reaction diagram
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recombinant enzyme shows 14.4% of the activity with Manalpha(1,2)Manalpha(1,6)[Manalpha(1,3)]Manalpha(1,6)[Manalpha(1,2)Manalpha(1,2)Manalpha(1,3)]Manbeta(1,4)GLcNAcbeta(1,4)GlcNAc-pyridylamine
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-
?
GDP-mannose + Manalpha(1,6)[Manalpha(1,3)]Manalpha(1,6)[Manalpha(1,2)Manalpha(1,3)]Manbeta(1,4)GlcNAcbeta(1,4)GlcNAc-pyridylamine
?
show the reaction diagram
-
recombinant enzyme shows 14.4% of the activity with Manalpha(1,2)Manalpha(1,6)[Manalpha(1,3)]Manalpha(1,6)[Manalpha(1,2)Manalpha(1,2)Manalpha(1,3)]Manbeta(1,4)GLcNAcbeta(1,4)GlcNAc-pyridylamine
-
-
?
GDP-mannose + Manalpha(1-2)Manalpha(1-6)[Manalpha(1-2)Manalpha(1-3)]Manalpha(1-6)[Manalpha(1-2)Manalpha(1-2)Manalpha(1-3)]Manbeta(1-4)GlcNAc
GDP + Manalpha(1-2)[Manalpha(1-6)]Manalpha(1-6)[Manalpha(1-2)Manalpha(1-3)]Manalpha(1-6)[Manalpha(1-2)Manalpha(1-2)Manalpha(1-3)]Manbeta(1-4)GlcNAc
show the reaction diagram
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Man9GlcNAc from rat liver glycoproteins and thyroglobulin, enzyme initiates outer chain synthesis, the mannose residue added is alpha-1,6-linked to the alpha-1,6-mannose residue of the substrate, removal of the alpha-1,2-linked mannose residue from Man9GlcNAc is not essential for enzyme activity
Man10GlcNAc
?
GDP-mannose + Manalpha(1-2)Manalpha(1-6)[Manalpha(1-3)]Manalpha(1-6)[Manalpha(1-2)Manalpha(1-2)Manalpha(1-3)]Manbeta(1-4)GlcNAc
GDP + Manalpha(1-2)[Manalpha(1-6)]Manalpha(1-6)[Manalpha(1-3)]Manalpha(1-6)[Manalpha(1-2)Manalpha(1-2)Manalpha(1-3)]Manbeta(1-4)GlcNAc
show the reaction diagram
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Man8GlcNAc from rat liver glycoproteins and thyroglobulin, enzyme initiates outer chain synthesis, the mannose residue added is alpha-1,6-linked to the alpha-1,6-mannose residue of the substrate
Man9GlcNAc
?
GDP-mannose + Manalpha(1-3)Manalpha(1-2)Manalpha(1-3)Manalpha(1-2)Man
GDP + Manalpha(1-3)[Manalpha(1-6)]Manalpha(1-2)Manalpha(1-2)Manalpha(1-2)Man
show the reaction diagram
GDP-mannose + oligosaccharide acceptor
?
show the reaction diagram
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Mnn9p is involved in protein mannosylation, incorporates alpha-1,6-linked mannose residues, the elongation of the N-glycan outer chain, initiated by Och1p, is continued by two mannosyltransferase complexes: V-complex composed of Mnn9p and Van1p and A-complex composed of Mnn9p, Anp1p, Hoc1p, Mnn10p and Mnn11p
-
?
GDP-mannose + oligosaccharide acceptor
GDP + alpha-mannosyl-1,6-oligosaccharide
show the reaction diagram
GDP-mannose + pyridylaminated Man5GlcNAc2
GDP + pyridylaminated Man6GlcNAc2
show the reaction diagram
incorporates alpha-1,6-linked mannose, poor substrate
-
?
GDP-mannose + pyridylaminated Man6GlcNAc2
GDP + pyridylaminated Man7GlcNAc2
show the reaction diagram
incorporates alpha-1,6-linked mannose
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?
GDP-mannose + pyridylaminated Man7GlcNAc2
GDP + pyridylaminated Man8GlcNAc2
show the reaction diagram
incorporates alpha-1,6-linked mannose
-
?
GDP-mannose + pyridylaminated Man8GlcNAc2
GDP + pyridylaminated Man9GlcNAc2
show the reaction diagram
incorporates alpha-1,6-linked mannose
-
?
GDP-mannose + pyridylaminated Man9GlcNAc2
GDP + pyridylaminated Man10GlcNAc2
show the reaction diagram
incorporates alpha-1,6-linked mannose
-
?
GDP-mannose + pyridylaminated oligosaccharide acceptor
?
show the reaction diagram
GDP-mannose + pyridylaminated oligosaccharide acceptor
GDP + pyridylaminated alpha-mannosyl-1,6-oligosaccharide
show the reaction diagram
incorporates alpha-1,6-linked mannose, substrate specificity
-
?
GDP-mannose + pyridylamino-Manalpha1-3Man-alpha1-2Manalpha1-2Manalpha1-2Man
?
show the reaction diagram
additional information
?
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NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
GDP-mannose + oligosaccharide acceptor
?
show the reaction diagram
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Mnn9p is involved in protein mannosylation, incorporates alpha-1,6-linked mannose residues, the elongation of the N-glycan outer chain, initiated by Och1p, is continued by two mannosyltransferase complexes: V-complex composed of Mnn9p and Van1p and A-complex composed of Mnn9p, Anp1p, Hoc1p, Mnn10p and Mnn11p
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?
additional information
?
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METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
Triton X-100
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requirement, maximum activity at 0.5-2%
additional information
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och1+ gene expression is induced by NaCl and KCl through the transcription factor Atf1p
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KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.35
Manalpha(1-2)Manalpha(1-6)[Manalpha(1-2)Manalpha(1-3)]Manalpha(1-6)[Manalpha(1-2)Manalpha(1-2)Manalpha(1-3)]Manbeta(1-4)GlcNAc
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-
0.39
Manalpha(1-2)Manalpha(1-6)[Manalpha(1-3)]Manalpha(1-6)[Manalpha(1-2)Manalpha(1-2)Manalpha(1-3)]Manbeta(1-4)GlcNAc
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-
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
additional information
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
7
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activity assay
7.1 - 7.6
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in Tris maleate buffer
pH RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
2 - 7
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relative activity at pH 2 28%, at pH 7 100%, strain J-1012 alpha-1,6-MT
6.5 - 8.5
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the recombinant Och1p in Tris/malate buffer exhibits above 80% of maximum activity between pH 6.5 and pH 8.5
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
TEMPERATURE RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
27 - 37
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strain J-1012 alpha-1,6-MT
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
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Mnn9p, type II Golgi membrane protein
Manually annotated by BRENDA team
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
additional information
-
sequence of Anl1p contains a putative transmembrane domain, enzyme interacts with YlMnn9p
Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
particulate enzyme fractions are obtained by centrifugation of Candida albicans cell homogenates
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recombinant enzyme
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Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
ANL1 and OCH1 gene
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as a fusion with the with green fluorescent protein, MNN9-OCH1 chimeric genes are constructed and cloned; as a fusion with the with red fluorescent protein, MNN9-OCH1 chimeric genes are constructed and cloned
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into the pGEMT-Easy vector
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MNN9 gene encoding Mnn9p is cloned and sequenced
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OCH1 gene encodes Och1p, an alpha-1,6-mannosyltransferase
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och1+ gene encodes alpha-1,6-mannosyltransferase, recombinant expression and cloning, och1+ gene knockout fission yeast
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och1+ gene, sequencing
recombinant Och1p is expressed in Pichia pastoris as a secreted protein, after truncation of its transmembrane region and fusion with myc and histidine tags at the C-terminus
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transcription of the OCH1 gene encoding alpha-1,6-mannosyltransferase is regulated by 2 transcription factors, Swi4 and Skn7, it is controlled by the ubiquitin-dependent degradation pathway
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transcriptional control of the och1+ gene, gene expression is not regulated during the cell cycle, but is induced by NaCl and KCl through the transcription factor Atf1p
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ENGINEERING
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
M16/O16
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chimeric Mnn9p-Och1p protein, a peptide from the first to the 16th amino acids of Mnn9p is fused with a polypeptide from the 16th to the 480th amino acids of Och1p
M33/O37
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chimeric Mnn9p-Och1p protein, a peptide from the first to the 33th amino acids of Mnn9p is fused with a polypeptide from the 37th to the 480th amino acids of Och1p
M93/O80
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chimeric Mnn9p-Och1p protein, a peptide from the first to the 93th amino acids of Mnn9p is fused with a polypeptide from the 80th to the 480th amino acids of Och1p
W266stop
inactive mutant strain 7X4 with a nonsense mutation resulting in a truncated Och1p, temperature- and hygromycin B-sensitive, no synthesis of outer chains on acid phosphatase
additional information
APPLICATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
drug development
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along with the possibility of a lack of the immunogenic terminal alpha-1,3-mannose linkages in Yarrowia lipolytica, a Yloch1delta mutant strain can be exploited as a platform strain for developing another potential yeast system that can produce recombinant glycoproteins with human-compatible oligosaccharides