Information on EC 2.4.1.232 - initiation-specific alpha-1,6-mannosyltransferase

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The expected taxonomic range for this enzyme is: Eukaryota

EC NUMBER
COMMENTARY
2.4.1.232
-
RECOMMENDED NAME
GeneOntology No.
initiation-specific alpha-1,6-mannosyltransferase
REACTION
REACTION DIAGRAM
COMMENTARY
ORGANISM
UNIPROT ACCESSION NO.
LITERATURE
Transfers an alpha-D-mannosyl residue from GDP-mannose into lipid-linked oligosaccharide, forming an alpha-(1->6)-D-mannosyl-D-mannose linkage
show the reaction diagram
requires Mn2+. In Saccharomyces cerevisiae, this enzyme catalyses an essential step in the outer chain elongation of N-linked oligosaccharides. Man8GlcNAc and Man9GlcNAc are equally good substrates
-
-
-
Transfers an alpha-D-mannosyl residue from GDP-mannose into lipid-linked oligosaccharide, forming an alpha-(1->6)-D-mannosyl-D-mannose linkage
show the reaction diagram
requires Mn2+, in Saccharomyces cerevisiae, this enzyme catalyses an essential step in the outer chain elongation of N-linked oligosaccharides, Man8GlcNAc and Man9GlcNAc are equally good substrates
Q9UTR6
REACTION TYPE
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
glycosyl group transfer
Q9UTR6
-
hexosyl group transfer
-
-
-
-
hexosyl group transfer
Q9UTR6
-
hexosyl group transfer
-
-
hexosyl group transfer
-
-
hexosyl group transfer
-
-
hexosyl group transfer
Candida albicans J-1012
-
-
-
PATHWAY
KEGG Link
MetaCyc Link
Metabolic pathways
-
Various types of N-glycan biosynthesis
-
SYSTEMATIC NAME
IUBMB Comments
GDP-mannose:oligosaccharide 6-alpha-D-mannosyltransferase
Requires Mn2+. In Saccharomyces cerevisiae, this enzyme catalyses an essential step in the outer chain elongation of N-linked oligosaccharides. Man8GlcNAc and Man9GlcNAc are equally good substrates.
SYNONYMS
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
alpha-1,6-mannosyltransferase
-
-
-
-
alpha-1,6-mannosyltransferase
-
-
alpha-1,6-mannosyltransferase
Candida albicans J-1012
-
-
-
alpha-1,6-mannosyltransferase
-
-
alpha-1,6-mannosyltransferase
Q9UTR6
-
alpha-1,6-mannosyltransferase
-
-
alpha-1,6-MT
-
-
alpha-1,6-MT
Candida albicans J-1012
-
-
-
alpha-mannosyltransferase
-
-
alpha-mannosyltransferase
Candida albicans J-1012
-
-
-
alpha1,6-mannosyltransferase KlOch1p
-
-
alpha1-6-mannosyltransferase
-
-
Anl1p
Q6C286
component of Golgi multi-protein complexes
Dol-P-Man:Man7GlcNAc2-PP-Dol-alpha1-6-mannosyltransferase
-
-
GDP-mannose:glycolipid 1,6-alpha-D-mannosyltransferase
-
-
-
-
GDP-mannose:glycolipid 1,6-alpha-D-mannosyltransferase
Q9UTR6
-
GDP-mannose:oligosaccharide 1,6-alpha-D-mannosyltransferase
-
-
-
-
glycolipid 6-alpha-mannosyltransferase
-
-
-
-
glycolipid 6-alpha-mannosyltransferase
Q9UTR6
-
glycoprotein alpha1-6-mannosyltransferase
Q9UTR6
-
HpOCH1
Q56VL2
-
initiation-specific alpha-1,6-mannosyltransferase
-
-
mannosyltransferase, guanosine diphosphomannose-glycoprotein alpha1-6-
Q9UTR6
-
Mnn9p
-
Saccharomyces cerevisiae
Mnn9p
-
Saccharomyces cerevisiae, part of two elongating alpha-1,6-mannosyltransferase complexes
Mnn9p
Q6C286
component of Golgi multi-protein complexes
Och1p
Q56VL2
-
Och1p
-
Saccharomyces cerevisiae, initiating alpha-1,6-mannosyltransferase
SpOch1p
Q9UTR6
och1+ gene product, Schizosaccharomyces pombe
CAS REGISTRY NUMBER
COMMENTARY
135622-87-6
-
346003-17-6
-, Schizosaccharomyces pombe gene och1+
ORGANISM
COMMENTARY
LITERATURE
SEQUENCE CODE
SEQUENCE DB
SOURCE
NIH B-792 serotype B strain
-
-
Manually annotated by BRENDA team
strain J-1012 and B-792
-
-
Manually annotated by BRENDA team
Candida albicans J-1012
strain J-1012 and B-792
-
-
Manually annotated by BRENDA team
Candida albicans NIH B-792 serotype B
NIH B-792 serotype B strain
-
-
Manually annotated by BRENDA team
mnn1 mutant lacking alpha-1,3-mannosyltransferase
-
-
Manually annotated by BRENDA team
Saccharomyces cerevisiae Och1p
Och1p
-
-
Manually annotated by BRENDA team
strains JY741 and L972
SwissProt
Manually annotated by BRENDA team
Schizosaccharomyces pombe Och1p
Och1p
-
-
Manually annotated by BRENDA team
gene ALG12
-
-
Manually annotated by BRENDA team
isoforms Anl1p and Och1p
-
-
Manually annotated by BRENDA team
GENERAL INFORMATION
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
malfunction
-
the variant surface glycoprotein sVSG221 synthesized by the ALG12-/- parasites shows different glycosylation patterns to that synthesized by wild-type cells, changes in sVSG221 glycosylation induced by the deletion of the ALG12 gene, overview
physiological function
-
the TbALG12 gene encodes the alpha1-6-mannosyltransferase that converts Man7GlcNAc2-PP-Dol to Man8GlcNAc2-PP-Dol
SUBSTRATE
PRODUCT                      
REACTION DIAGRAM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
(Substrate)
LITERATURE
(Substrate)
COMMENTARY
(Product)
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
alpha-D-mannose + Manalpha(1,6)[Manalpha(1,3)]Manalpha(1,6)[Manalpha(1,2)Manalpha(1,2)Manalpha(1,3)]Manbeta(1,4)GlcNAcbeta(1,4)GlcNAc-pyridylamine
?
show the reaction diagram
-
recombinant enzyme shows 28.8% of the activity with Manalpha(1,2)Manalpha(1,6)[Manalpha(1,3)]Manalpha(1,6)[Manalpha(1,2)Manalpha(1,2)Manalpha(1,3)]Manbeta(1,4)GLcNAcbeta(1,4)GlcNAc-pyridylamine
-
-
?
GDP-mannose + alpha-1,6-D-mannobiose
?
show the reaction diagram
-
two distinct Mnn9p-containing multi-protein complexes add multiple mannosyl residues forming a series of poly-mannose structures in vitro, no substrate of Och1p
formation of a series of poly-mannose structures, which contain mainly alpha-1,6- and some additional alpha-1,2-linked mannoses
?
GDP-mannose + Man8GlcNAc
GDP + Man9GlcNAc
show the reaction diagram
-
Man8GlcNAc from thyroglobulin Man9GlcNAc by treatment with yeast specific mannosidase, specificity, enzyme catalyzes addition of mannose to the alpha-1,3-mannose of the substrate, initiates outer chain formation
-
?
GDP-mannose + Man8GlcNAc2-PA
GDP + Man9GlcNAc2-PA
show the reaction diagram
Saccharomyces cerevisiae, Saccharomyces cerevisiae Och1p
-
best acceptor for Och1p, initiation-specific alpha-1,6-mannosyltransferase that requires the intact structure of Man8GlcNAc for efficient mannose outer chain initiation
additional Man is attached with an alpha-1,6-linkage at the site where mannose outer chain elongation initiates
?
GDP-mannose + Man9GlcNAc
GDP + Man10GlcNAc
show the reaction diagram
-
Man9GlcNAc from thyroglobulin, specificity, enzyme catalyzes addition of mannose to the alpha-1,3-mannose of the substrate, initiates outer chain formation
-
?
GDP-mannose + Man9GlcNAc2-PA
GDP + Man10GlcNAc2-PA
show the reaction diagram
Schizosaccharomyces pombe, Schizosaccharomyces pombe Och1p
-
enzyme incorporates alpha-1,6-linked mannose into the substrate
-
-
?
GDP-mannose + Manalpha(1,2)Manalpha(1,6)[Manalpha(1,2)Manalpha(1,3)]Manalpha(1,6)[Manalpha(1,2)Manalpha(1,2)Manalpha(1,3)]Manbeta(1,4)GlcNAcbeta(1,4)GlcNAc-pyridylamine
?
show the reaction diagram
-
recombinant enzyme shows 74.2% of the activity with Manalpha(1,2)Manalpha(1,6)[Manalpha(1,3)]Manalpha(1,6)[Manalpha(1,2)Manalpha(1,2)Manalpha(1,3)]Manbeta(1,4)GLcNAcbeta(1,4)GlcNAc-pyridylamine. Och1p has the catalytic potential to transfer two molecules of mannose to Manalpha(1,2)Manalpha(1,6)[Manalpha(1,2)Manalpha(1,3)]Manalpha(1,6)[Manalpha(1,2)Manalpha(1,2)Manalpha(1,3)]Manbeta(1,4)GlcNAcbeta(1,4)GlcNAc-pyridylamine. The second mannose is attached with alpha1,6-linkage to either the alpha1,6-linked or the alpha1,3-linked mannose that is attached to alpha1,6-linked mannose
-
-
?
GDP-mannose + Manalpha(1,2)Manalpha(1,6)[Manalpha(1,2)Manalpha(1,3)]Manalpha(1,6)[Manalpha(1,2)Manalpha(1,3)]Manbeta(1,4)GlcNAcbeta(1,4)GlcNAc-pyridylamine
?
show the reaction diagram
-
recombinant enzyme shows 58.5% of the activity with Manalpha(1,2)Manalpha(1,6)[Manalpha(1,3)]Manalpha(1,6)[Manalpha(1,2)Manalpha(1,2)Manalpha(1,3)]Manbeta(1,4)GLcNAcbeta(1,4)GlcNAc-pyridylamine
-
-
?
GDP-mannose + Manalpha(1,2)Manalpha(1,6)[Manalpha(1,3)]Manalpha(1,6)[Manalpha(1,2)Manalpha(1,2)Manalpha(1,3)]Manbeta(1,4)GlcNAcbeta(1,4)GlcNAc-pyridylamine
?
show the reaction diagram
-
-
-
-
?
GDP-mannose + Manalpha(1,2)Manalpha(1,6)[Manalpha(1,3)]Manalpha(1,6)[Manalpha(1,2)Manalpha(1,3)]Manbeta(1,4)GlcNAcbeta(1,4)GlcNAc-pyridylamine
?
show the reaction diagram
-
recombinant enzyme shows 66.1% of the activity with Manalpha(1,2)Manalpha(1,6)[Manalpha(1,3)]Manalpha(1,6)[Manalpha(1,2)Manalpha(1,2)Manalpha(1,3)]Manbeta(1,4)GLcNAcbeta(1,4)GlcNAc-pyridylamine
-
-
?
GDP-mannose + Manalpha(1,6)[Manalpha(1,2)Manalpha(1,3)]Manalpha(1,6)[Manalpha(1,2)Manalpha(1,2)Manalpha(1,3)]Manbeta(1,4)GlcNAcbeta(1,4)GlcNAc-pyridylamine
?
show the reaction diagram
-
recombinant enzyme shows 28.8% of the activity with Manalpha(1,2)Manalpha(1,6)[Manalpha(1,3)]Manalpha(1,6)[Manalpha(1,2)Manalpha(1,2)Manalpha(1,3)]Manbeta(1,4)GLcNAcbeta(1,4)GlcNAc-pyridylamine
-
-
?
GDP-mannose + Manalpha(1,6)[Manalpha(1,2)Manalpha(1,3)]Manalpha(1,6)[Manalpha(1,2)Manalpha(1,3)]Manbeta(1,4)GlcNAcbeta(1,4)GlcNAc-pyridylamine
?
show the reaction diagram
-
recombinant enzyme shows 14.4% of the activity with Manalpha(1,2)Manalpha(1,6)[Manalpha(1,3)]Manalpha(1,6)[Manalpha(1,2)Manalpha(1,2)Manalpha(1,3)]Manbeta(1,4)GLcNAcbeta(1,4)GlcNAc-pyridylamine
-
-
?
GDP-mannose + Manalpha(1,6)[Manalpha(1,3)]Manalpha(1,6)[Manalpha(1,2)Manalpha(1,3)]Manbeta(1,4)GlcNAcbeta(1,4)GlcNAc-pyridylamine
?
show the reaction diagram
-
recombinant enzyme shows 14.4% of the activity with Manalpha(1,2)Manalpha(1,6)[Manalpha(1,3)]Manalpha(1,6)[Manalpha(1,2)Manalpha(1,2)Manalpha(1,3)]Manbeta(1,4)GLcNAcbeta(1,4)GlcNAc-pyridylamine
-
-
?
GDP-mannose + Manalpha(1-2)Manalpha(1-6)[Manalpha(1-2)Manalpha(1-3)]Manalpha(1-6)[Manalpha(1-2)Manalpha(1-2)Manalpha(1-3)]Manbeta(1-4)GlcNAc
GDP + Manalpha(1-2)[Manalpha(1-6)]Manalpha(1-6)[Manalpha(1-2)Manalpha(1-3)]Manalpha(1-6)[Manalpha(1-2)Manalpha(1-2)Manalpha(1-3)]Manbeta(1-4)GlcNAc
show the reaction diagram
-
Man9GlcNAc from rat liver glycoproteins and thyroglobulin, enzyme initiates outer chain synthesis, the mannose residue added is alpha-1,6-linked to the alpha-1,6-mannose residue of the substrate, removal of the alpha-1,2-linked mannose residue from Man9GlcNAc is not essential for enzyme activity
Man10GlcNAc
?
GDP-mannose + Manalpha(1-2)Manalpha(1-6)[Manalpha(1-3)]Manalpha(1-6)[Manalpha(1-2)Manalpha(1-2)Manalpha(1-3)]Manbeta(1-4)GlcNAc
GDP + Manalpha(1-2)[Manalpha(1-6)]Manalpha(1-6)[Manalpha(1-3)]Manalpha(1-6)[Manalpha(1-2)Manalpha(1-2)Manalpha(1-3)]Manbeta(1-4)GlcNAc
show the reaction diagram
-
Man8GlcNAc from rat liver glycoproteins and thyroglobulin, enzyme initiates outer chain synthesis, the mannose residue added is alpha-1,6-linked to the alpha-1,6-mannose residue of the substrate
Man9GlcNAc
?
GDP-mannose + Manalpha(1-3)Manalpha(1-2)Manalpha(1-3)Manalpha(1-2)Man
GDP + Manalpha(1-3)[Manalpha(1-6)]Manalpha(1-2)Manalpha(1-2)Manalpha(1-2)Man
show the reaction diagram
Candida albicans, Candida albicans NIH B-792 serotype B
-
mannopentaose from Candida albicans, substrate specificity study, structural requirement of enzyme is Manalpha(1-3)Manalpha(1-)
structure, presence of an alpha-1,6 branching mannose unit
?
GDP-mannose + oligosaccharide acceptor
GDP + alpha-mannosyl-1,6-oligosaccharide
show the reaction diagram
-
Mnn9p incorporates alpha-1,6-linked mannose residues, the elongation of the N-glycan outer chain, initiated by Och1p, is continued by two mannosyltransferase complexes: V-complex composed of Mnn9p and Van1p and A-complex composed of Mnn9p, Anp1p, Hoc1p, Mnn10p and Mnn11p, study of the interactions between the subunits of the mannosyltransferase complexes
-
?
GDP-mannose + oligosaccharide acceptor
GDP + alpha-mannosyl-1,6-oligosaccharide
show the reaction diagram
-
Mnn9p is a component of two distinct multi-protein complexes, both having alpha-1,6-mannosyltransferase activity: a complex of Mnn9p and Van1p and a complex of Mnn9p, Anp1p, Mnn10p, Mnn11p and Hoc1p
-
?
GDP-mannose + oligosaccharide acceptor
GDP + alpha-mannosyl-1,6-oligosaccharide
show the reaction diagram
-
Mnn9p is associated in two distinct multi-protein complexes, both having alpha-1,6-mannosyltransferase activity, they add multiple mannosyl residues forming hypermannose structures, Och1p adds the initiating alpha-1,6-mannose residue to an N-linked Man8GlcNAc2 structure, the Mnn9p-containing protein complexes add further alpha-1,6-mannose residues synthesizing the backbone of the outer chain of N-linked glycans
-
?
GDP-mannose + oligosaccharide acceptor
?
show the reaction diagram
-
Mnn9p is involved in protein mannosylation, incorporates alpha-1,6-linked mannose residues, the elongation of the N-glycan outer chain, initiated by Och1p, is continued by two mannosyltransferase complexes: V-complex composed of Mnn9p and Van1p and A-complex composed of Mnn9p, Anp1p, Hoc1p, Mnn10p and Mnn11p
-
?
GDP-mannose + pyridylaminated Man5GlcNAc2
GDP + pyridylaminated Man6GlcNAc2
show the reaction diagram
Q9UTR6
incorporates alpha-1,6-linked mannose, poor substrate
-
?
GDP-mannose + pyridylaminated Man6GlcNAc2
GDP + pyridylaminated Man7GlcNAc2
show the reaction diagram
Q9UTR6
incorporates alpha-1,6-linked mannose
-
?
GDP-mannose + pyridylaminated Man7GlcNAc2
GDP + pyridylaminated Man8GlcNAc2
show the reaction diagram
Q9UTR6
incorporates alpha-1,6-linked mannose
-
?
GDP-mannose + pyridylaminated Man8GlcNAc2
GDP + pyridylaminated Man9GlcNAc2
show the reaction diagram
Q9UTR6
incorporates alpha-1,6-linked mannose
-
?
GDP-mannose + pyridylaminated Man9GlcNAc2
GDP + pyridylaminated Man10GlcNAc2
show the reaction diagram
Q9UTR6
incorporates alpha-1,6-linked mannose
-
?
GDP-mannose + pyridylaminated oligosaccharide acceptor
?
show the reaction diagram
-
substrate specificity
-
-
?
GDP-mannose + pyridylaminated oligosaccharide acceptor
?
show the reaction diagram
-
substrate specificity, SpOch1p
-
-
?
GDP-mannose + pyridylaminated oligosaccharide acceptor
?
show the reaction diagram
-
various high mannose-type oligosaccharides as acceptors, Man8GlcNAc2-PA is the best acceptor, the loss of 1 or 2 alpha-1,2-mannoses from Man8GlcNAc2 reduces the activity
-
-
?
GDP-mannose + pyridylaminated oligosaccharide acceptor
?
show the reaction diagram
Saccharomyces cerevisiae Och1p
-
substrate specificity, various high mannose-type oligosaccharides as acceptors, Man8GlcNAc2-PA is the best acceptor, the loss of 1 or 2 alpha-1,2-mannoses from Man8GlcNAc2 reduces the activity
-
-
?
GDP-mannose + pyridylaminated oligosaccharide acceptor
?
show the reaction diagram
Schizosaccharomyces pombe Och1p
-
substrate specificity, SpOch1p
-
-
?
GDP-mannose + pyridylaminated oligosaccharide acceptor
GDP + pyridylaminated alpha-mannosyl-1,6-oligosaccharide
show the reaction diagram
Q9UTR6
incorporates alpha-1,6-linked mannose, substrate specificity
-
?
GDP-mannose + pyridylamino-Manalpha1-3Man-alpha1-2Manalpha1-2Manalpha1-2Man
?
show the reaction diagram
Candida albicans, Candida albicans J-1012
-
substrate for activity assay
-
-
?
additional information
?
-
-
enzyme also transfers the alpha-1,6-linked branching mannose unit to the mannan of Saccharomyces cerevisiae
-
-
-
additional information
?
-
-
no acceptors for Och1p: Man5GlcNAc2 completely lacking alpha-1,2-Man, Man8GlcNAcOH
-
-
-
additional information
?
-
-
Mnn9p, no acceptor: alpha-1,2-mannobiose
-
?
additional information
?
-
-
Och1p adds one alpha-1,6-linked mannose residue to the core glycan of glycoproteins, e.g. pro-carboxypeptidase Y, the soluble cell wall protein Scw4p
-
?
additional information
?
-
Q9UTR6
SpOch1p has a wider substrate specificity than ScOch1p from Saccharomyces cerevisiae
-
?
additional information
?
-
-
enzyme initiates outer chain formation of oligosaccharides
-
-
-
additional information
?
-
-
Och1p is functional in the initiation of alpha-1,6-polymannose outer chain addition to the N-linked core oligosaccharide in yeast, Man5GlcNAc2 and Man8GlcNAc2
-
-
-
additional information
?
-
-
Mnn9p is required for the addition of the long alpha-1,6-mannose backbone of the complex mannan and for the complex glycosylation of secreted proteins
-
-
-
additional information
?
-
-
enzyme is required for the initiation of outer chain elongation of N-linked oligosaccharides of cell wall mannoproteins, transcriptional control of the och1+ gene, gene expression is not regulated during the cell cycle, but is induced by salt stress through the transcription factor Atf1p
-
-
-
additional information
?
-
-
OCH1 gene encoded enzyme initiates the polymannose outer chain elongation of N-glycans, regulation of the OCH1 transcription
-
-
-
additional information
?
-
-
involved in glycoprotein biosynthesis, enzyme catalyzes the first step specific to N-linked oligosaccharide synthesis and the biosynthesis of the outer chain of mannoproteins
-
-
-
additional information
?
-
-
SpOch1p is a key enzyme of outer chain elongation of N-linked oligosaccharides
-
-
-
additional information
?
-
-
Och1p is essential for the outer chain elongation of N-linked oligosaccharides
-
-
-
additional information
?
-
-
Mnn9p is involved in the formation of mannan chains in the Golgi
-
?
additional information
?
-
-
Och1p is responsible for starting the extension of the primary N-glycans in the Golgi by addition of an alpha-1,6-mannose residue to the core glycan of glycoproteins
-
?
additional information
?
-
Q9UTR6
SpOch1p is a key enzyme in outer chain elongation of N-linked oligosaccharides on glycoproteins
-
?
additional information
?
-
-
two distinct Mnn9p-containing multi-protein complexes are responsible for the synthesis and initial branching of the long alpha-1,6-linked backbone of the hypermannose structure attached to many yeast glycoproteins, the long alpha-1,6-linked backbone is attached to an initial alpha-1,6-mannose residue transferred to the core structure by Och1p
-
?
additional information
?
-
-
involved in N-glycosylation pathway
-
-
-
additional information
?
-
-, Q56VL2
HpOCH1 is the key enzyme responsible for initiating N-linked outer chain biosynthesis by adding the first alpha-1,6-mannose residue onto the core oligosaccharide
-
-
-
additional information
?
-
-
the enzyme is required for cell-wall organization and proper functioning of the secretory pathway
-
-
-
additional information
?
-
-
the N-linked oligosaccharide modification in the Golgi apparatus is initiated by alpha1,6-mannosyltransferase (encoded by the OCH1 gene) with the addition of mannose to the Man8GlcNAc2 or Man9GlcNAc2 endoplasmic reticulum intermediates, Och1p has the potential to transfer two mannoses from GDP-mannose
-
-
-
additional information
?
-
-
transfers an alpha-D-mannosyl residue from GDP-mannose into lipid-linked oligosaccharide, forming an alpha-(1-6)-D-mannosyl-D-mannose linkage
-
-
-
additional information
?
-
-
transfers an alpha-D-mannosyl residue from GDP-mannose into lipid-linked oligosaccharide, forming an alpha-(1-6)-D-mannosyl-D-mannose linkage
-
-
-
additional information
?
-
-
transfers an alpha-D-mannosyl residue from GDP-mannose into lipid-linked oligosaccharide, forming an alpha-(1-6)-D-mannosyl-D-mannose linkage
-
-
-
additional information
?
-
Saccharomyces cerevisiae Och1p
-
Och1p is functional in the initiation of alpha-1,6-polymannose outer chain addition to the N-linked core oligosaccharide in yeast, Man5GlcNAc2 and Man8GlcNAc2
-
-
-
additional information
?
-
Saccharomyces cerevisiae Och1p
-
OCH1 gene encoded enzyme initiates the polymannose outer chain elongation of N-glycans, regulation of the OCH1 transcription
-
-
-
additional information
?
-
Saccharomyces cerevisiae Och1p
-
no acceptors for Och1p: Man5GlcNAc2 completely lacking alpha-1,2-Man, Man8GlcNAcOH, Och1p is essential for the outer chain elongation of N-linked oligosaccharides
-
-
-
additional information
?
-
Saccharomyces cerevisiae A
-
Mnn9p is required for the addition of the long alpha-1,6-mannose backbone of the complex mannan and for the complex glycosylation of secreted proteins
-
-
-
additional information
?
-
Schizosaccharomyces pombe Och1p
-
enzyme is required for the initiation of outer chain elongation of N-linked oligosaccharides of cell wall mannoproteins, transcriptional control of the och1+ gene, gene expression is not regulated during the cell cycle, but is induced by salt stress through the transcription factor Atf1p
-
-
-
additional information
?
-
Schizosaccharomyces pombe Och1p
-
SpOch1p is a key enzyme of outer chain elongation of N-linked oligosaccharides
-
-
-
additional information
?
-
Candida albicans J-1012
-
transfers an alpha-D-mannosyl residue from GDP-mannose into lipid-linked oligosaccharide, forming an alpha-(1-6)-D-mannosyl-D-mannose linkage
-
-
-
additional information
?
-
Candida albicans NIH B-792 serotype B
-
enzyme also transfers the alpha-1,6-linked branching mannose unit to the mannan of Saccharomyces cerevisiae
-
-
-
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
(Substrate)
LITERATURE
(Substrate)
COMMENTARY
(Product)
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
GDP-mannose + oligosaccharide acceptor
?
show the reaction diagram
-
Mnn9p is involved in protein mannosylation, incorporates alpha-1,6-linked mannose residues, the elongation of the N-glycan outer chain, initiated by Och1p, is continued by two mannosyltransferase complexes: V-complex composed of Mnn9p and Van1p and A-complex composed of Mnn9p, Anp1p, Hoc1p, Mnn10p and Mnn11p
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?
additional information
?
-
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enzyme initiates outer chain formation of oligosaccharides
-
-
-
additional information
?
-
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Och1p is functional in the initiation of alpha-1,6-polymannose outer chain addition to the N-linked core oligosaccharide in yeast, Man5GlcNAc2 and Man8GlcNAc2
-
-
-
additional information
?
-
-
Mnn9p is required for the addition of the long alpha-1,6-mannose backbone of the complex mannan and for the complex glycosylation of secreted proteins
-
-
-
additional information
?
-
-
enzyme is required for the initiation of outer chain elongation of N-linked oligosaccharides of cell wall mannoproteins, transcriptional control of the och1+ gene, gene expression is not regulated during the cell cycle, but is induced by salt stress through the transcription factor Atf1p
-
-
-
additional information
?
-
-
OCH1 gene encoded enzyme initiates the polymannose outer chain elongation of N-glycans, regulation of the OCH1 transcription
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-
-
additional information
?
-
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involved in glycoprotein biosynthesis, enzyme catalyzes the first step specific to N-linked oligosaccharide synthesis and the biosynthesis of the outer chain of mannoproteins
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-
-
additional information
?
-
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SpOch1p is a key enzyme of outer chain elongation of N-linked oligosaccharides
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-
-
additional information
?
-
-
Och1p is essential for the outer chain elongation of N-linked oligosaccharides
-
-
-
additional information
?
-
-
Mnn9p is involved in the formation of mannan chains in the Golgi
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?
additional information
?
-
-
Och1p is responsible for starting the extension of the primary N-glycans in the Golgi by addition of an alpha-1,6-mannose residue to the core glycan of glycoproteins
-
?
additional information
?
-
Q9UTR6
SpOch1p is a key enzyme in outer chain elongation of N-linked oligosaccharides on glycoproteins
-
?
additional information
?
-
-
two distinct Mnn9p-containing multi-protein complexes are responsible for the synthesis and initial branching of the long alpha-1,6-linked backbone of the hypermannose structure attached to many yeast glycoproteins, the long alpha-1,6-linked backbone is attached to an initial alpha-1,6-mannose residue transferred to the core structure by Och1p
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?
additional information
?
-
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involved in N-glycosylation pathway
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-
-
additional information
?
-
-, Q56VL2
HpOCH1 is the key enzyme responsible for initiating N-linked outer chain biosynthesis by adding the first alpha-1,6-mannose residue onto the core oligosaccharide
-
-
-
additional information
?
-
-
the enzyme is required for cell-wall organization and proper functioning of the secretory pathway
-
-
-
additional information
?
-
-
the N-linked oligosaccharide modification in the Golgi apparatus is initiated by alpha1,6-mannosyltransferase (encoded by the OCH1 gene) with the addition of mannose to the Man8GlcNAc2 or Man9GlcNAc2 endoplasmic reticulum intermediates
-
-
-
additional information
?
-
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transfers an alpha-D-mannosyl residue from GDP-mannose into lipid-linked oligosaccharide, forming an alpha-(1-6)-D-mannosyl-D-mannose linkage
-
-
-
additional information
?
-
-
transfers an alpha-D-mannosyl residue from GDP-mannose into lipid-linked oligosaccharide, forming an alpha-(1-6)-D-mannosyl-D-mannose linkage
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-
-
additional information
?
-
-
transfers an alpha-D-mannosyl residue from GDP-mannose into lipid-linked oligosaccharide, forming an alpha-(1-6)-D-mannosyl-D-mannose linkage
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-
-
additional information
?
-
Saccharomyces cerevisiae Och1p
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Och1p is functional in the initiation of alpha-1,6-polymannose outer chain addition to the N-linked core oligosaccharide in yeast, Man5GlcNAc2 and Man8GlcNAc2
-
-
-
additional information
?
-
Saccharomyces cerevisiae Och1p
-
OCH1 gene encoded enzyme initiates the polymannose outer chain elongation of N-glycans, regulation of the OCH1 transcription
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-
-
additional information
?
-
Saccharomyces cerevisiae Och1p
-
Och1p is essential for the outer chain elongation of N-linked oligosaccharides
-
-
-
additional information
?
-
Saccharomyces cerevisiae A
-
Mnn9p is required for the addition of the long alpha-1,6-mannose backbone of the complex mannan and for the complex glycosylation of secreted proteins
-
-
-
additional information
?
-
Schizosaccharomyces pombe Och1p
-
enzyme is required for the initiation of outer chain elongation of N-linked oligosaccharides of cell wall mannoproteins, transcriptional control of the och1+ gene, gene expression is not regulated during the cell cycle, but is induced by salt stress through the transcription factor Atf1p
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-
-
additional information
?
-
Schizosaccharomyces pombe Och1p
-
SpOch1p is a key enzyme of outer chain elongation of N-linked oligosaccharides
-
-
-
additional information
?
-
Candida albicans J-1012
-
transfers an alpha-D-mannosyl residue from GDP-mannose into lipid-linked oligosaccharide, forming an alpha-(1-6)-D-mannosyl-D-mannose linkage
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-
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METALS and IONS
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
Mn2+
-
absolute requirement, optimum concentration: 10 mM, Ca2+, Mg2+, Co2+ or Zn2+ cannot substitute for Mn2+
Mn2+
-
no activity without addition of Mn2+
ACTIVATING COMPOUND
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
IMAGE
Triton X-100
-
requirement, maximum activity at 0.5-2%
additional information
-
och1+ gene expression is induced by NaCl and KCl through the transcription factor Atf1p
-
KM VALUE [mM]
KM VALUE [mM] Maximum
SUBSTRATE
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
IMAGE
0.35
-
Manalpha(1-2)Manalpha(1-6)[Manalpha(1-2)Manalpha(1-3)]Manalpha(1-6)[Manalpha(1-2)Manalpha(1-2)Manalpha(1-3)]Manbeta(1-4)GlcNAc
-
-
0.39
-
Manalpha(1-2)Manalpha(1-6)[Manalpha(1-3)]Manalpha(1-6)[Manalpha(1-2)Manalpha(1-2)Manalpha(1-3)]Manbeta(1-4)GlcNAc
-
-
SPECIFIC ACTIVITY [µmol/min/mg]
SPECIFIC ACTIVITY MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
additional information
-
Q9UTR6
-
additional information
-
-
relative activity at 27C 81%, at 37C 27%, strain B-792 alpha-1,6-MT; relative activity at 27C 87%, at 37C 46%, strain J-1012 alpha-1,6-MT
pH OPTIMUM
pH MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
7
-
-
activity assay
7.1
7.6
-
in Tris maleate buffer
7.2
-
-
assay at
7.5
-
Q9UTR6
assay at
7.5
-
-
recombinant enzyme
7.5
-
-
in vitro alpha-1,6-mannosyltransferase activity assay
pH RANGE
pH RANGE MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
2
7
-
relative activity at pH 2 28%, at pH 7 100%, strain J-1012 alpha-1,6-MT
6.5
8.5
-
the recombinant Och1p in Tris/malate buffer exhibits above 80% of maximum activity between pH 6.5 and pH 8.5
TEMPERATURE OPTIMUM
TEMPERATURE OPTIMUM MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
30
-
Q9UTR6
assay at
30
-
-
in vitro alpha-1,6-mannosyltransferase activity assay
30
-
-
activity assay
TEMPERATURE RANGE
TEMPERATURE MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
27
37
-
strain J-1012 alpha-1,6-MT
LOCALIZATION
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
GeneOntology No.
LITERATURE
SOURCE
-
enzyme recycles between the Golgi and the endoplasmic reticulum, Och1p functions in the endoplasmic reticulum when allowed to accumulate there together with substrate proteins
Manually annotated by BRENDA team
-
the Mnn9p-containing multi-protein complexes cycle back and forth between the endoplasmic reticulum and Golgi, type II transmembrane protein
Manually annotated by BRENDA team
-
Mnn9p, primarily localized to the cis Golgi, type II transmembrane protein
Manually annotated by BRENDA team
-
enzyme recycles between the Golgi and the endoplasmic reticulum, but normally functions in the cis-Golgi
Manually annotated by BRENDA team
-
Mnn9p, cis Golgi, the Mnn9p-containing multi-protein complexes cycle back and forth between the endoplasmic reticulum and Golgi, type II transmembrane protein
Manually annotated by BRENDA team
-
Mnn9p, type II Golgi membrane protein
Manually annotated by BRENDA team
-
Mnn9p is a membrane-associated protein, type II transmembrane protein
Manually annotated by BRENDA team
Saccharomyces cerevisiae A
-
Mnn9p is a membrane-associated protein, type II transmembrane protein
-
Manually annotated by BRENDA team
SUBUNITS
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
additional information
-
sequence of Anl1p contains a putative transmembrane domain, enzyme interacts with YlMnn9p
Purification/COMMENTARY
ORGANISM
UNIPROT ACCESSION NO.
LITERATURE
particulate enzyme fractions are obtained by centrifugation of Candida albicans cell homogenates
-
recombinant enzyme
-
Cloned/COMMENTARY
ORGANISM
UNIPROT ACCESSION NO.
LITERATURE
as a fusion with the with green fluorescent protein, MNN9-OCH1 chimeric genes are constructed and cloned; as a fusion with the with red fluorescent protein, MNN9-OCH1 chimeric genes are constructed and cloned
-
MNN9 gene encoding Mnn9p is cloned and sequenced
-
OCH1 gene encodes Och1p, an alpha-1,6-mannosyltransferase
-
recombinant Och1p is expressed in Pichia pastoris as a secreted protein, after truncation of its transmembrane region and fusion with myc and histidine tags at the C-terminus
-
transcription of the OCH1 gene encoding alpha-1,6-mannosyltransferase is regulated by 2 transcription factors, Swi4 and Skn7, it is controlled by the ubiquitin-dependent degradation pathway
-
och1+ gene encodes alpha-1,6-mannosyltransferase, recombinant expression and cloning, och1+ gene knockout fission yeast
-
och1+ gene, sequencing
Q9UTR6
transcriptional control of the och1+ gene, gene expression is not regulated during the cell cycle, but is induced by NaCl and KCl through the transcription factor Atf1p
-
ANL1 and OCH1 gene
-
into the pGEMT-Easy vector
-
ENGINEERING
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
M16/O16
-
chimeric Mnn9p-Och1p protein, a peptide from the first to the 16th amino acids of Mnn9p is fused with a polypeptide from the 16th to the 480th amino acids of Och1p
M33/O37
-
chimeric Mnn9p-Och1p protein, a peptide from the first to the 33th amino acids of Mnn9p is fused with a polypeptide from the 37th to the 480th amino acids of Och1p
M93/O80
-
chimeric Mnn9p-Och1p protein, a peptide from the first to the 93th amino acids of Mnn9p is fused with a polypeptide from the 80th to the 480th amino acids of Och1p
additional information
-
Mnn9 mutant strain
additional information
-
och1 mutants, och1 mutation causes a complete loss of the alpha-1,6-polymannose outer chain
additional information
-
DELTAmnn9 mutant with N-glycosylation defect, null mutant, chimera Mnn9 proteins
additional information
-
strain with deletion of the OCH1 gene
additional information
Saccharomyces cerevisiae A
-
Mnn9 mutant strain
-
additional information
Saccharomyces cerevisiae Och1p
-
och1 mutants, och1 mutation causes a complete loss of the alpha-1,6-polymannose outer chain
-
additional information
-
och1+ gene deficient strain shows loss of mannose transfer from GDP-mannose to Man9GlcNAc2-PA and loss of high-mannose type sugar chain glycosylation, och1 mutant that does not synthesize the outer chains containing mannose on acid phosphatase
additional information
Q9UTR6
disruption mutant of och1+: viable, slow-growing phenotype, temperature- and hygromycin B-sensitive, no synthesis of outer chains on acid phosphatase
W266stop
Q9UTR6
inactive mutant strain 7X4 with a nonsense mutation resulting in a truncated Och1p, temperature- and hygromycin B-sensitive, no synthesis of outer chains on acid phosphatase
additional information
Schizosaccharomyces pombe Och1p
-
och1+ gene deficient strain shows loss of mannose transfer from GDP-mannose to Man9GlcNAc2-PA and loss of high-mannose type sugar chain glycosylation, och1 mutant that does not synthesize the outer chains containing mannose on acid phosphatase
-
additional information
-
OCH1 and Anl1 deletion strains, increased sensitivity to sodium dodecyl sulfate, and to Calcofluor White
APPLICATION
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
drug development
-
along with the possibility of a lack of the immunogenic terminal alpha-1,3-mannose linkages in Yarrowia lipolytica, a Yloch1delta mutant strain can be exploited as a platform strain for developing another potential yeast system that can produce recombinant glycoproteins with human-compatible oligosaccharides