Information on EC 2.4.1.226 - N-acetylgalactosaminyl-proteoglycan 3-beta-glucuronosyltransferase

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The expected taxonomic range for this enzyme is: Amniota

EC NUMBER
COMMENTARY hide
2.4.1.226
-
RECOMMENDED NAME
GeneOntology No.
N-acetylgalactosaminyl-proteoglycan 3-beta-glucuronosyltransferase
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
UDP-alpha-D-glucuronate + N-acetyl-beta-D-galactosaminyl-(1->4)-beta-D-glucuronosyl-proteoglycan = UDP + beta-D-glucuronosyl-(1->3)-N-acetyl-beta-D-galactosaminyl-(1->4)-beta-D-glucuronosyl-proteoglycan
show the reaction diagram
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
glucuronyl group transfer
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glycosyl group transfer
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PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
chondroitin biosynthesis
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Glycosaminoglycan biosynthesis - chondroitin sulfate / dermatan sulfate
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Metabolic pathways
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SYSTEMATIC NAME
IUBMB Comments
UDP-alpha-D-glucuronate:N-acetyl-beta-D-galactosaminyl-(1->4)-beta-D-glucuronosyl-proteoglycan 3-beta-glucuronosyltransferase
Involved in the biosynthesis of chondroitin and dermatan sulfate. The human chondroitin synthetase is a bifunctional glycosyltransferase, which has the 3-beta-glucuronosyltransferase and 4-beta-N-acetylgalactosaminyltransferase (EC 2.4.1.175) activities required for the synthesis of the chondroitin sulfate disaccharide repeats. Similar chondroitin synthase 'co-polymerases' can be found in Pasteurella multocida and Escherichia coli. There is also another human protein with apparently only the 3-beta-glucuronosyltransferase activity.
CAS REGISTRY NUMBER
COMMENTARY hide
176023-59-9
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269077-98-7
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ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
white leghorn
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-
Manually annotated by BRENDA team
Mus musculus C57/BL6
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-
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Manually annotated by BRENDA team
Wistar rats
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Manually annotated by BRENDA team
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
malfunction
physiological function
additional information
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
UDP-alpha-D-glucuronate + alpha-thrombomodulin
UDP + beta-D-glucuronosyl-(1,3)-alpha-thrombomodulin
show the reaction diagram
-
-
-
?
UDP-alpha-D-glucuronate + beta-D-glucuronosyl-(1,3)-beta-D-galactosyl-(1,3)-beta-D-galactosyl-1,4-beta-D-xylose-1-O-Gly-Ser-Gly-Glu
UDP + ?
show the reaction diagram
synthetic tetrasaccharide substrate
-
-
?
UDP-alpha-D-glucuronate + beta-D-glucuronosyl-(1,3)-beta-D-galactosyl-(1,3)-beta-D-galactosyl-1,4-beta-D-xylose-1-O-serine
UDP + ?
show the reaction diagram
synthetic tetrasaccharide substrate
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-
?
UDP-alpha-D-glucuronate + chondroitin
UDP + beta-D-glucuronosyl-(1,3)-chondroitin
show the reaction diagram
UDP-alpha-D-glucuronate + chondroitin sulfate A
UDP + beta-D-glucuronosyl-(1,3)-chondroitin sulfate A
show the reaction diagram
and oligosaccharide of chondroitin sulfate with chain length of C11
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-
?
UDP-alpha-D-glucuronate + N-acetyl-beta-D-galactosaminyl-(1-4)-beta-D-glucuronosyl-proteoglycan
UDP + beta-D-glucuronosyl-(1-3)-N-acetyl-beta-D-galactosaminyl-(1-4)-beta-D-glucuronosyl-proteoglycan
show the reaction diagram
UDP-alpha-D-glucuronate + N-acetyl-beta-D-galactosaminyl-(1->4)-beta-D-glucuronosyl-proteoglycan
UDP + beta-D-glucuronosyl-(1->3)-N-acetyl-beta-D-galactosaminyl-(1->4)-beta-D-glucuronosyl-proteoglycan
show the reaction diagram
UDP-glucuronic acid + chondroitin
UDP + N-acetylchondrosine glucuronic acid beta(1-3)N-acetylgalactosamine
show the reaction diagram
UDP-glucuronic acid + chondroitin heptasaccharide
?
show the reaction diagram
heptasaccharide having a N-acetylgalactosamine residue at its non-reducing terminus
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?
UDP-glucuronic acid + chondroitin polysaccharide
?
show the reaction diagram
polysaccharide substrate used after beta-glucuronidase treatment
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-
?
UDP-glucuronic acid + chondroitin sulfate heptasaccharide
?
show the reaction diagram
heptasaccharide having a N-acetylgalactosamine residue at its non-reducing terminus
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-
?
UDP-glucuronic acid + chondroitin sulfate undecasaccharide
?
show the reaction diagram
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?
additional information
?
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NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
UDP-alpha-D-glucuronate + chondroitin
UDP + beta-D-glucuronosyl-(1,3)-chondroitin
show the reaction diagram
Q8IZ52
chondroitin polymerization in disaccharide units by glucuronyltransferase and N-acetylgalactosaminyltransferase activities of the enzyme
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-
?
UDP-alpha-D-glucuronate + N-acetyl-beta-D-galactosaminyl-(1-4)-beta-D-glucuronosyl-proteoglycan
UDP + beta-D-glucuronosyl-(1-3)-N-acetyl-beta-D-galactosaminyl-(1-4)-beta-D-glucuronosyl-proteoglycan
show the reaction diagram
UDP-alpha-D-glucuronate + N-acetyl-beta-D-galactosaminyl-(1->4)-beta-D-glucuronosyl-proteoglycan
UDP + beta-D-glucuronosyl-(1->3)-N-acetyl-beta-D-galactosaminyl-(1->4)-beta-D-glucuronosyl-proteoglycan
show the reaction diagram
additional information
?
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METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Co2+
17% and 31% as effective as Mn2+ for glucuronyltransferase and N-acetylgalactosaminyltransferase activities, respectively
ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
chondroitin synthase-1
co-expressed with chondroitin synthase-1 CSS3 also exhibits polymerase activity
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chondroitin-polymerizing factor
co-expressed with ChPF (chondroitin-polymerizing factor) enzyme shows chondroitin polymerase activity
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KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.027
C11-oligosaccharide of chondroitin sulfate A
pH 6.2, 37C, CSS2
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0.0653
chondroitin sulfate undecasaccharide
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0.263
UDP-alpha-D-glucuronate
pH 6.2, 37C, CSS2
0.051 - 0.0824
UDP-glucuronic acid
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
0.00000004
glucuronyltransferase of recombinant wild-type enzyme secreted from COS-1 cells
0.0000006
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recombinant CSS2B variant in COS-7 cells, pH 6.2, 37C
0.0000024
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recombinant CSS2A variant in COS-7 cells, pH 6.2, 37C
0.00000314
glucuronyltransferase of recombinant mutant fusion protein consisting of enzyme and chondroitin polymerizing factor secreted from COS-1 cells
additional information
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
5.5 - 6
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sodium acetate buffer
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
CSS2, high level expression
Manually annotated by BRENDA team
CSS2, high level expression
Manually annotated by BRENDA team
CSS2, high level expression
Manually annotated by BRENDA team
CSS2, high level expression
Manually annotated by BRENDA team
CSS2, high level expression
Manually annotated by BRENDA team
additional information
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
enzyme is a type II membrane protein
Manually annotated by BRENDA team
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
additional information
Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
a soluble form of CSGlcA-T is generated by replacing the first 57 amino acids of the protein with a cleavable insulin signal sequence and a protein A IgG-binding domain. The soluble protein is expressed in COS-1 cells as a recombinant protein fused with the protein A IgG-binding domain. The fusion protein secreted into the medium is adsorbed onto IgG-Sepharose beads for purification to eliminate endogenous glycosyltransferases, and then the protein-bound beads are used as an enzyme source
partially
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recombinant FLAG-tagged soluble catalytic domain from COS-7 cells
Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
CSGlcAT/ChSy-3 quantitative tissue expression analysis by realtime RT-PCR, overview
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DNA and amino acid sequence determination and analysis, expression of putative catalytic domains of CSGlcAT-II and CSS2 in COS-7 cells as FLAG-tagged soluble proteins, which possesses glucuronyltransferase and N-acetylgalactosaminyltransferase activities as the wild-type enzyme
enzyme domain containing beta-3-glycosyltransferase motifs, expressed in COS-7 cells ATCCCRL 1651
expressed in COS-1 cells; expressed in COS-1 cells
genetic organization, coexpression of enzyme and chondroitin polymerizing factor as fusion protein in COS-1 cells, secretion into the cell culture medium
individual overexpression of soluble forms of FLAG- and Myc-tagged splice variants CSS2A and CSS2B in COS-7 cells, co-expression with CSS1. Whereas CSS2A facilitates chondroitin sulfate biosynthesis, CSS2B inhibits it. Analysis of transcription levels of CSS2 variants
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RT-PCR expression analysis of CHSY1, CHSY3 and CSGlcA-T from macroscopically normal and pathological specimens, overview
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ENGINEERING
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
D184A
mutant protein which lacks any glycosyltransferase activity but interacts with other ChSy family members shows that the glycosyltransferase activity of CSGlcA-T plays an important role in chondroitin polymerization
additional information
APPLICATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
medicine
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