Information on EC 2.4.1.22 - lactose synthase

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The expected taxonomic range for this enzyme is: Eukaryota, Bacteria

EC NUMBER
COMMENTARY
2.4.1.22
-
RECOMMENDED NAME
GeneOntology No.
lactose synthase
REACTION
REACTION DIAGRAM
COMMENTARY
ORGANISM
UNIPROT ACCESSION NO.
LITERATURE
UDP-alpha-D-galactose + D-glucose = UDP + lactose
show the reaction diagram
-
-
-
-
UDP-alpha-D-galactose + D-glucose = UDP + lactose
show the reaction diagram
random or ordered bi bi mechanism
-
REACTION TYPE
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
hexosyl group transfer
-
-
-
-
PATHWAY
KEGG Link
MetaCyc Link
Galactose metabolism
-
Metabolic pathways
-
SYSTEMATIC NAME
IUBMB Comments
UDP-galactose:D-glucose 4-beta-D-galactosyltransferase
The enzyme is a complex of two proteins, A and B. In the absence of the B protein (alpha-lactalbumin), the enzyme catalyses the transfer of galactose from UDP-alpha-D-galactose to N-acetylglucosamine (EC 2.4.1.90 N-acetyllactosamine synthase).
SYNONYMS
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
alpha-lactalbumin
-
a subunit of lactose-synthase
beta-1,4-galactosyltransferase
-
-
beta-GalT
-
-
beta1,4-galactosyltransferase
-
-
beta1,4-galactosyltransferase-1
-
-
beta4Gal-T1
-
-
c-LacdiNAcS
-
exhibits N-acetylgalactosaminyltransferase activity in the absence of exogenous alpha-lactalbumin as well as other glycosyltransferase activities including lactose synthase, and beta1,4-galactosyltransferase 1
GalNAc(beta1-4)GlcNAc synthase
-
-
glactosyltransferase, uridine diphosphogalactose-glucose
-
-
-
-
lacdiNAc
-
-
LACS
-
-
lactose synthase
-
-
lactose synthetase
-
-
-
-
LALBA
-
a subunit of lactose-synthase
N-acetyllactosamine synthase
-
-
-
-
UDP-galactose-glucose galactosyltransferase
-
-
-
-
UDPgalactose-glucose galactosyltransferase
-
-
-
-
UGT72B1
-
-
additional information
-
the lactose synthase complex is composed of alpha-lactalbumin and beta1,4-galactosyltransferase components
CAS REGISTRY NUMBER
COMMENTARY
9030-11-9
-
ORGANISM
COMMENTARY
LITERATURE
SEQUENCE CODE
SEQUENCE DB
SOURCE
long isoform of alpha-lactalbumin; South African fur seal
UniProt
Manually annotated by BRENDA team
short isoform of alpha-lactalbumin; South African fur seal
UniProt
Manually annotated by BRENDA team
long isoform of alpha-lactalbumin; subantarctic fur seal
UniProt
Manually annotated by BRENDA team
short isoform of alpha-lactalbumin; subantarctic fur seal
UniProt
Manually annotated by BRENDA team
Ayrshire, Brown Swiss, Holstein, and Jersey cows
-
-
Manually annotated by BRENDA team
catalytic domain of recombinant bovine beta4Gal-T1 from residues 130 to 402, d129 beta4Gal-T1, 33000 Da, and mouse recombinant alpha-lactalbumin
-
-
Manually annotated by BRENDA team
alpha-lactalbumin fragment; Northern fur seal
UniProt
Manually annotated by BRENDA team
alpha-lactalbumin; Weddell seal
A7TWS8
UniProt
Manually annotated by BRENDA team
female
-
-
Manually annotated by BRENDA team
alpha-lactalbumin fragment; Australian sea lion
UniProt
Manually annotated by BRENDA team
alpha-lactalbumin; atlantic walrus
UniProt
Manually annotated by BRENDA team
alpha-lactalbumin fragment; i.e. Pusa hispida, ringed seal
UniProt
Manually annotated by BRENDA team
the beta1,4-galactosyltransferase-I component is encoded by gene B4GALT1
-
-
Manually annotated by BRENDA team
alpha-lactalbumin; California sealion
UniProt
Manually annotated by BRENDA team
GENERAL INFORMATION
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
malfunction
-
sequence variations of bovine alpha-lactalbumin are associated with differences in milk yield
SUBSTRATE
PRODUCT                      
REACTION DIAGRAM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
(Substrate)
LITERATURE
(Substrate)
COMMENTARY
(Product)
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
alpha-UDP-D-galactose + D-glucose
UDP + lactose
show the reaction diagram
-
inversion of anomeric configuration to form beta-O-galactosides
-
-
?
alpha-UDP-D-galactose + N-acetyl-D-glucosamine
UDP + alpha-D-galactosyl(1-4)beta-N-acetyl-D-glucosamine
show the reaction diagram
-
-
-
-
?
dUDPgalactose + D-glucose
lactose + dUDP
show the reaction diagram
-
-
-
-
?
dUDPgalactose + D-glucose
lactose + dUDP
show the reaction diagram
-
-
-
-
?
UDP-D-galactose + D-glucose
UDP + lactose
show the reaction diagram
-
lactose synthesis is achieved only in the presence of alpha-lactalbumin. In absence of alpha-lactalbumin the enzyme catalyzes the reaction of EC 2.4.1.90
-
-
?
UDP-galactose + D-glucose
UDP + lactose
show the reaction diagram
-
-
-
-
?
UDP-galactose + D-glucose
UDP + lactose
show the reaction diagram
-
-
-
-
?
UDP-galactose + D-glucose
UDP + lactose
show the reaction diagram
-
-
-
-
?
UDP-galactose + D-glucose
UDP + lactose
show the reaction diagram
-
-
-
-
?
UDP-galactose + D-glucose
UDP + lactose
show the reaction diagram
A7TWT0
-
-
-
?
UDP-galactose + D-glucose
UDP + lactose
show the reaction diagram
A7TWS1, A7TWS2
-
-
-
?
UDP-galactose + D-glucose
UDP + lactose
show the reaction diagram
A7TWS3, A7TWS4
-
-
-
?
UDP-galactose + D-glucose
UDP + lactose
show the reaction diagram
A7TWS5
-
-
-
?
UDP-galactose + D-glucose
UDP + lactose
show the reaction diagram
A7TWS6
-
-
-
?
UDP-galactose + D-glucose
UDP + lactose
show the reaction diagram
A7TWS7
-
-
-
?
UDP-galactose + D-glucose
UDP + lactose
show the reaction diagram
A7TWS8
-
-
-
?
UDP-galactose + D-glucose
UDP + lactose
show the reaction diagram
A7TWS5
-
-
-
?
UDP-galactose + D-glucose
UDP + lactose
show the reaction diagram
-
in presence of alpha-lactalbumin
-
-
?
UDP-galactose + D-glucose
UDP + lactose
show the reaction diagram
-
formation of a beta(1-4)linkage
-
-
?
UDP-galactose + D-glucose
UDP + lactose
show the reaction diagram
A7TWT0
residues necessary for lactose synthesis activity are Phe31, His32, Leu110, Gln117 and Trp118 of alpha-lactalbumin
-
-
?
UDP-galactose + D-glucose
UDP + lactose
show the reaction diagram
A7TWS1, A7TWS2
residues necessary for lactose synthesis activity are Phe31, His32, Leu110, Gln117 and Trp118 of alpha-lactalbumin
-
-
?
UDP-galactose + D-glucose
UDP + lactose
show the reaction diagram
A7TWS3, A7TWS4
residues necessary for lactose synthesis activity are Phe31, His32, Leu110, Gln117 and Trp118 of alpha-lactalbumin
-
-
?
UDP-galactose + D-glucose
UDP + lactose
show the reaction diagram
A7TWS5
residues necessary for lactose synthesis activity are Phe31, His32, Leu110, Gln117 and Trp118 of alpha-lactalbumin
-
-
?
UDP-galactose + D-glucose
UDP + lactose
show the reaction diagram
A7TWS6
residues necessary for lactose synthesis activity are Phe31, His32, Leu110, Gln117 and Trp118 of alpha-lactalbumin
-
-
?
UDP-galactose + D-glucose
UDP + lactose
show the reaction diagram
A7TWS7
residues necessary for lactose synthesis activity are Phe31, His32, Leu110, Gln117 and Trp118 of alpha-lactalbumin
-
-
?
UDP-galactose + D-glucose
UDP + lactose
show the reaction diagram
A7TWS8
residues necessary for lactose synthesis activity are Phe31, His32, Leu110, Gln117 and Trp118 of alpha-lactalbumin
-
-
?
UDP-galactose + D-glucose
UDP + lactose
show the reaction diagram
A7TWS5
residues necessary for lactose synthesis activity are Phe31, His32, Leu110, Gln117 and Trp118 of alpha-lactalbumin
-
-
?
UDP-galactose + N-acetyl-D-glucosamine
UDP + D-galactosyl(1-4)beta-N-acetyl-D-glucosamine
show the reaction diagram
-
-
-
-
?
UDP-galactose + N-acetyl-D-glucosamine
UDP + D-galactosyl(1-4)beta-N-acetyl-D-glucosamine
show the reaction diagram
-
-
-
-
?
UDP-glucose + N-acetyl-D-glucosamine
UDP + D-glucosyl(1-4)beta-N-acetyl-D-glucosamine
show the reaction diagram
-
about 20% activity compared to UDP-galactose
-
-
?
UDPgalactose + alpha-methyl-D-glucose
?
show the reaction diagram
-
-
-
-
?
UDPgalactose + cellobiose
?
show the reaction diagram
-
7.5% of the activity of glucose
-
-
?
UDPgalactose + D-glucose
lactose + UDP
show the reaction diagram
-
-
-
-
?
UDPgalactose + D-glucose
lactose + UDP
show the reaction diagram
-
-
-
-
?
UDPgalactose + D-glucose
lactose + UDP
show the reaction diagram
-
-
-
-
?
UDPgalactose + D-glucose
lactose + UDP
show the reaction diagram
-
-
-
-
?
UDPgalactose + D-glucose
lactose + UDP
show the reaction diagram
-
-
-
-
?
UDPgalactose + D-glucose
lactose + UDP
show the reaction diagram
-
-
-
-
?
UDPgalactose + D-glucose
lactose + UDP
show the reaction diagram
-
-
-
-
?
UDPgalactose + D-glucose
lactose + UDP
show the reaction diagram
-
-
-
-
?
UDPgalactose + D-glucose
lactose + UDP
show the reaction diagram
-
-
-
-
?
UDPgalactose + D-glucose
lactose + UDP
show the reaction diagram
-
the galactosyl transferase can catalyze the transfer of galactose to glucose to form lactose in the absence of alpha-lactalbumin provided the glucose concentration is high
-
-
?
UDPgalactose + D-xylose
?
show the reaction diagram
-
-
-
-
?
UDPgalactose + gentiobiose
?
show the reaction diagram
-
-
-
-
?
UDPgalactose + glucose
lactose + UDP
show the reaction diagram
-
biosynthesis of lactose
-
-
?
UDPgalactose + maltose
?
show the reaction diagram
-
-
-
-
?
dUDPgalactose + D-glucose
lactose + dUDP
show the reaction diagram
-
dUDPgalactose is 80% as effective as UDP-D-galactose
-
-
?
additional information
?
-
-
acceptor substrate specificity, overview
-
-
-
additional information
?
-
-
upon substrate binding to beta4Gal-T1, a large conformational change occurs in the region comprising residues 345 to 365. The role of alpha-lactalbumin is to hold glucose by hydrogen bonding with the O-1 hydroxyl group in the acceptor-binding site on beta4Gal-T1, while the N-acetyl group-binding pocket in beta4Gal-T1 adjusts to maximize the interactions with the glucose molecule
-
-
-
additional information
?
-
-
acceptor substrate specificity, no activity with D-galactose, N-acetylgalactosamine, glucosamine, and lactose as acceptor substrates, no activity with UDP-glucose as donor substrate, overview
-
-
-
additional information
?
-
-
acceptor substrate specificity, no activity with D-galactose, N-acetylgalactosamine, glucosamine, and lactose as acceptor substrates, overview
-
-
-
additional information
?
-
-
molecular interaction with different donor substrates, overview, the enzyme undergoes transition from open to close conformation upon binding of donor or acceptor substrate, overview
-
-
-
additional information
?
-
-
beta1,4-galactosyltransferase-I forms a complex with alpha-lactalbumin to give lactose synthase
-
-
-
additional information
?
-
-
c-LacdiNAcS is auto-lacdiNAcylated and can synthesize GalNAc(beta1-4)GlcNAc structures on cellular glycoproteins
-
-
-
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
(Substrate)
LITERATURE
(Substrate)
COMMENTARY
(Product)
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
UDP-galactose + D-glucose
UDP + lactose
show the reaction diagram
-
-
-
-
?
UDP-galactose + D-glucose
UDP + lactose
show the reaction diagram
-
-
-
-
?
UDP-galactose + D-glucose
UDP + lactose
show the reaction diagram
A7TWT0
-
-
-
?
UDP-galactose + D-glucose
UDP + lactose
show the reaction diagram
A7TWS1, A7TWS2
-
-
-
?
UDP-galactose + D-glucose
UDP + lactose
show the reaction diagram
A7TWS3, A7TWS4
-
-
-
?
UDP-galactose + D-glucose
UDP + lactose
show the reaction diagram
A7TWS5
-
-
-
?
UDP-galactose + D-glucose
UDP + lactose
show the reaction diagram
A7TWS6
-
-
-
?
UDP-galactose + D-glucose
UDP + lactose
show the reaction diagram
A7TWS7
-
-
-
?
UDP-galactose + D-glucose
UDP + lactose
show the reaction diagram
A7TWS8
-
-
-
?
UDP-galactose + D-glucose
UDP + lactose
show the reaction diagram
A7TWS5
-
-
-
?
UDPgalactose + glucose
lactose + UDP
show the reaction diagram
-
biosynthesis of lactose
-
-
?
METALS and IONS
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
Ca2+
-
25% of the activation with Mn2+
Mg2+
-
25% of the activation with Mn2+
Mn2+
-
required, partially replaceable by Mg2+, Ca2+
Mn2+
-
not replaceable by Mg2+, Ca2+
Mn2+
-
required, Km: 0.2 mM
Mn2+
-
required, optimal metal cofactor at 0.1 mM MnCl2
Mn2+
-
required
INHIBITORS
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
IMAGE
2,4,6-Trinitrobenzenesulfonate
-
modification of alpha-lactalbumin
2-deoxy-D-glucose
-
0.4 M, 68% inhibition
2-Hydroxy-5-nitrobenzyl bromide
-
modification of alpha-lactalbumin
2-mercaptoethanol
-
modification of alpha-lactalbumin
2-Nitrophenylsulfenyl chloride
-
modification of alpha-lactalbumin
acetone
-
20% v/v, 69% inhibition
acetonitrile
-
20% v/v, complete inhibition
alpha-Lactalbumin
-
inhibits galactosyl transfer to N-acetylglucosamine
-
Ca2+
-
above 4 mM
carbodiimide
-
at pH 4.5 rapid loss of the biological activity of alpha-lactalbumin
D-arabinose
-
0.4 M, 13% inhibition
D-ribose
-
0.4 M, 59% inhibition
D-xylose
-
0.4 M, 36% inhibition
Dimethyl-(2-hydroxy-5-nitrobenzyl)-sulfonium bromide
-
modification of alpha-lactalbumin
Dimethylsulfoxide
-
20% v/v, 43% inhibition
Dioxane
-
20% v/v, 88% inhibition
dithiothreitol
-
the rate of reduction of the disulfide bond in alpha-lactalbumin is much faster with dithiothreitol than mercaptoethanol, and at 24°C proceeds to completion within minutes at pH 8
ethanol
-
20% v/v, 4% inhibition
glycinamide
-
at pH 4.5 rapid loss of the biological activity of alpha-lactalbumin
iodoacetate
-
modification of alpha-lactalbumin, carboxymethylation proceeds in the order His68, His32, His107. The activity decreases progressively as carboxymethylation proceeds, the fully carboxymethylated derivative has about 40% of the activity of the unmodified alpha-lactalbumin in lactose synthetase assay
L-arabinose
-
0.4 M, 54% inhibition
L-sorbose
-
0.4 M, 45% inhibition
L-Xylose
-
0.4 M, 16% inhibition
Maleic anhydride
-
-
N,N-Dimethylformamide
-
20% v/v, 90% inhibition
N-methylpyrrolidone
-
20% v/v, complete inhibition
tetrahydrofuran
-
20% v/v, complete inhibition
Tetranitromethane
-
-
UDPglucose
-
-
Mg2+
-
above 4 mM
additional information
-
alpha-lactalbumin strongly inhibits the transfer of galactose to free N-acetylglucosamine
-
ACTIVATING COMPOUND
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
IMAGE
alpha-Lactalbumin
-
alters the acceptor specificity of the galactosyltransferase and thereby increases lactose synthesis and decreases N-acetylglucosamine synthesis
-
alpha-Lactalbumin
-
enhances equally the binding of N-acetylglucosamine or glucose to the galactosyl transferase
-
methanol
-
20% v/v, 17% activation
KM VALUE [mM]
KM VALUE [mM] Maximum
SUBSTRATE
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
IMAGE
1.7
-
glucose
-
-
1.8
-
glucose
-
serum
2
-
glucose
-
milk
2.3
-
glucose
-
liver, Golgi apparatus
3.4
-
glucose
-
-
0.009
-
UDPgalactose
-
serum
0.023
-
UDPgalactose
-
liver, Golgi apparatus
0.06
-
UDPgalactose
-
milk
1400
-
glucose
-
reaction in absence of alpha-lactalbumin
additional information
-
additional information
-
-
-
additional information
-
additional information
-
-
-
additional information
-
additional information
-
-
-
additional information
-
additional information
-
steady-state kinetics for random and ordered bi bi mechanism, overview
-
TURNOVER NUMBER [1/s]
TURNOVER NUMBER MAXIMUM[1/s]
SUBSTRATE
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
IMAGE
additional information
-
additional information
-
-
-
SPECIFIC ACTIVITY [µmol/min/mg]
SPECIFIC ACTIVITY MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
additional information
-
-
-
additional information
-
-
assay
additional information
-
-
-
additional information
-
-
-
additional information
-
-
development of a simple and fast pH-sensitive assay method using phenol red as pH-indicator, activities at different temperatures, overview
additional information
-
-
development of a simple and fast pH-sensitive assay method using phenol red as pH-indicator, overview
additional information
-
-
development of a high-throughput mass spectrometry multisubstrate assay method
additional information
-
-
beta1,4-galactosyltransferase in in milk of cows from several breeds at various stages of lactation, overview
pH OPTIMUM
pH MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
TEMPERATURE OPTIMUM
TEMPERATURE OPTIMUM MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
TEMPERATURE RANGE
TEMPERATURE MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
SOURCE TISSUE
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
SOURCE
-
alpha-lactalbumin, a mammary-specific protein
Manually annotated by BRENDA team
-
concentrations of lactose synthase components alpha-lactalbumin and beta1,4-galactosyltransferase in milk of cows from several breeds at various stages of lactation, overview
Manually annotated by BRENDA team
LOCALIZATION
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
GeneOntology No.
LITERATURE
SOURCE
-
trans-Golgi complex, beta1,4-galactosyltransferase-I
Manually annotated by BRENDA team
-
Golgi apparatus, galactosyl-transferase
Manually annotated by BRENDA team
-
B4GALT1 is a type II transmembrane enzyme
Manually annotated by BRENDA team
-
alpha-lactalbumin in synthesized on the rough endoplasmic reticulum
Manually annotated by BRENDA team
-
alpha-lactalbumin is a soluble milk protein secreted throughout lactation
-
Manually annotated by BRENDA team
MOLECULAR WEIGHT
MOLECULAR WEIGHT MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
50000
-
-
recombinant enzyme, gel filtration
60000
-
-
complex between galactosyl transferase and alpha-lactalbumin, equilibrium sedimentation
75000
-
-
gel filtration
SUBUNITS
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
?
-
x * 50000, recombinant enzyme, SDS-PAGE
dimer
-
1 * 14500, alpha-lactalbumin, + 1 * 46000, galactosyl transferase, equilibrium sedimentation
additional information
-
MW of lactalbumin determined by nucleotide sequence MW of lactalbumin determined by nucleotide sequence: 14179 Da; the three main polypeptides determined by SDS-PAGE of galactosyl transferase are active: 54000 Da, 48000 Da and 42000 Da
additional information
-
MW of lactalbumin determined by nucleotide sequence: guinea pig
additional information
-
MW of lactalbumin determined by nucleotide sequence MW of lactalbumin determined by nucleotide sequence: 14071 Da
additional information
-
the enzyme is composed of two structurally and functionally dissimilar proteins: 1. UDP-galactose:N-acetylglucosamine-beta4-galactosyl transferase, EC 2.4.1.90. Its normal function is to catalyze the incorporation of galactose into 1,4-linkage with N-acetylglucosamine during the synthesis of oliogosaccharide prosthetic groups of certain glycoproteins, free N-acetylglucosamine also serves as an excellent acceptor for galactose leading to the formation of N-acetylgalactosamine, 2. alpha-lactalbumin, interaction of alpha-lactalbumin with the transferase leads to lactose synthesis when UDP-galactose and glucose are present
additional information
-
galactosyl transferase component has a MW of 49000 Da, SDS-PAGE
additional information
-
-
additional information
-
the enzyme is 1.1 complex of a catalytic component beta1,4-galactosyltransferase (beta4gal-T1) and a regulatory component, alpha-lactalbumin
POSTTRANSLATIONAL MODIFICATION
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
glycoprotein
-
isolation of minor amounts of alpha-lactalbumin in milk which contains carbohydrate prosthetic group. Galactosyl transferase contains a considerable proportion of oligosaccharides
glycoprotein
-
-
glycoprotein
-
the glycoprotein contains 1.1% sialic acid, 5.03% galactosamine, 2.47% glucosamine, 2.36% galactose, 0.87% mannose and 0.87% fucose
glycoprotein
-
galactosyl transferase component contains 11% carbohydrate
glycoprotein
-
-
glycoprotein
-
N-glycosylation of B4GALT1
Crystallization/COMMENTARY
ORGANISM
UNIPROT ACCESSION NO.
LITERATURE
catalytic domain of recombinant bovine beta4Gal-T1 from residues 130 to 402, d129 beta4Gal-T1, 33000 Da, and mouse recombinant alpha-lactalbumin. Crystal structure of enzyme bound with various substrates
-
wild-type and mutant enzyme in closed conformation induced by UDP-galactose, X-ray diffraction structure determination and analysis at 2.3 A resolution, re-refinement or previously determined crystal structures of enzyme complexed with different substrates and Mn2+, overview
-
catalytic domain of recombinant bovine beta4Gal-T1 from residues 130 to 402, d129 beta4Gal-T1, 33000 Da, and mouse recombinant alpha-lactalbumin. Crystal structure of enzyme bound with various substrates
-
Purification/COMMENTARY
ORGANISM
UNIPROT ACCESSION NO.
LITERATURE
galactosyl transferase component
-
recombinant enzyme from Escherichia coli strain BL21(DE3)
-
recombinant enzyme fro Escherichia coli
-
galactosyl transferase component
-
recombinant enzyme fro Escherichia coli
-
Cloned/COMMENTARY
ORGANISM
UNIPROT ACCESSION NO.
LITERATURE
DNA and amino acid sequence determination and analysis of alpha-lactalbumin component of the lactose synthase complex, aberrant splicing is responsible for the two cDNA variants isolated from mammary tissue, with the longer variant maintaining the third intron and the shorter variant, phylogenetic analysis; DNA and amino acid sequence determination and analysis of alpha-lactalbumin component of the lactose synthase complex, aberrant splicing is responsible for the two cDNA variants isolated from mammary tissue, with the longer variant maintaining the third intron and the shorter variant, phylogenetic analysis
A7TWS3, A7TWS4
DNA and amino acid sequence determination and analysis of alpha-lactalbumin component of the lactose synthase complex, aberrant splicing is responsible for the two cDNA variants isolated from mammary tissue, with the longer variant maintaining the third intron and the shorter variant, phylogenetic analysis; DNA and amino acid sequence determination and analysis of alpha-lactalbumin component of the lactose synthase complex, aberrant splicing is responsible for the two cDNA variants isolated from mammary tissue, with the longer variant maintaining the third intron and the shorter variant, phylogenetic analysis
A7TWS1, A7TWS2
expressed in Escherichia Lec8 Chinese hamster ovary cells
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expression in Escherichia coli
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DNA and amino acid sequence determination and analysis of alpha-lactalbumin component of the lactose synthase complex, phylogenetic analysis
A7TWS5
expression in Escherichia coli
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DNA and amino acid sequence determination and analysis of alpha-lactalbumin component of the lactose synthase complex, phylogenetic analysis
A7TWS8
expression in Escherichia coli
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DNA and amino acid sequence determination and analysis of alpha-lactalbumin component of the lactose synthase complex, phylogenetic analysis
A7TWS7
DNA and amino acid sequence determination and analysis of alpha-lactalbumin component of the lactose synthase complex, phylogenetic analysis
A7TWT0
DNA and amino acid sequence determination and analysis of alpha-lactalbumin component of the lactose synthase complex, phylogenetic analysis
A7TWS5
gene B4GALT1, DNA and amino acid sequence determination and analysis, RT-PCR expression analysis, sequence comparison
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DNA and amino acid sequence determination and analysis of alpha-lactalbumin component of the lactose synthase complex, phylogenetic analysis
A7TWS6
ENGINEERING
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
C342T
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determination and analysis of the closed conformation crystal structure of enzyme mutant in complex with UDP-galactose