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Information on EC 2.4.1.207 - xyloglucan:xyloglucosyl transferase and Organism(s) Actinidia deliciosa and UniProt Accession C0IRG6

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EC Tree
     2 Transferases
         2.4 Glycosyltransferases
             2.4.1 Hexosyltransferases
                2.4.1.207 xyloglucan:xyloglucosyl transferase
IUBMB Comments
Does not use cello-oligosaccharides as either donor or acceptor.
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This record set is specific for:
Actinidia deliciosa
UNIPROT: C0IRG6
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The taxonomic range for the selected organisms is: Actinidia deliciosa
The expected taxonomic range for this enzyme is: Eukaryota, Bacteria
Synonyms
sixth, xyloglucan endotransglycosylase, xyloglucan endotransglucosylase/hydrolase, xyloglucan endotransglucosylase, xyloglucan endotransglycosylase/hydrolase, xth31, xyloglucan endo-transglycosylase, atxth21, xth33, xth19, more
SYNONYM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
endoxyloglucan transferase
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xyloglucan endotransglucosylase/hydrolase
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xyloglucan endotransglycosylase
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endo-xyloglucan transferase
-
-
-
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endoxyloglucan transferase
EXT
-
-
-
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GenBank L46792-derived protein GI 950299
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-
-
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GenBank X93173-derived protein GI 1890573
-
-
-
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GenBank X93174-derived protein GI 1890575
-
-
-
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Genbank X93175-derived protein GI 1890577
-
-
-
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GenBank Z97335-derived protein GI
-
-
-
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NXET
-
-
-
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xyloglucan endotransglucosylase/hydrolase
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xyloglucan endotransglycosylase
xyloglucan endotransglycosylase (Actinia deliciosa strain Hayward pericarp clone AdXET-5 gene XET precursor)
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-
-
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xyloglucan endotransglycosylase (Arabidopsis thaliana gene TCH4 precursor reduced)
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-
-
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xyloglucan endotransglycosylase (barley clone PM5 gene HVPM5)
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-
-
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xyloglucan endotransglycosylase (barley clone XEA gene HVXEA)
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-
-
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xyloglucan endotransglycosylase (barley clone XEB gene HVXEB)
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-
-
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xyloglucan endotransglycosylase-related protein XTR-7 (Arabidopsis thaliana)
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-
-
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xyloglucan recombinase
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-
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xyloglucan X93174-derived protein GI 1890575
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-
-
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xyloglucan-specific endo-(1->4)-beta-D-glucanase
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-
-
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xyloglucan:xyloglucanotransferase
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-
-
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xyloglucanotransferase, xyloglucan (xyloglucan donor)
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-
-
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xyloglucanotransferase, xyloglucan (xyloglucan donor) (Actinia chinensis clone AdXET-5 gene XET precursor)
-
-
-
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xyloglucanotransferase, xyloglucan (xyloglucan donor) (Arabidopsis thaliana gene TCH4 precursor reduced)
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-
-
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xyloglucanotransferase, xyloglucan (xyloglucan donor) (barley clone PM5 gene HVPM5)
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-
-
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xyloglucanotransferase, xyloglucan (xyloglucan donor) (barley clone XEA gene HVXEA)
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-
-
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xyloglucanotransferase, xyloglucan (xyloglucan donor) (barley clone XEB gene HVXEB)
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-
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SYSTEMATIC NAME
IUBMB Comments
xyloglucan:xyloglucan xyloglucanotransferase
Does not use cello-oligosaccharides as either donor or acceptor.
CAS REGISTRY NUMBER
COMMENTARY hide
141588-40-1
-
172279-31-1
xyloglucanotransferase, xyloglucan (xyloglucan donor) (Arabidopsis thaliana gene TCH4 precursor reduced) /xyloglucan endotransglycosylase (Arabidopsis thaliana gene TCH4 precursor reduced)
202012-34-8
xyloglucan endotransglycosylase-related protein XTR-7 (Arabidopsis thaliana) /genBank Z97335-derived protein GI 2244769
203402-40-8
xyloglucanotransferase, xyloglucan (xyloglucan donor) (barley clone PM5 gene HVPM5) /genBank X93173-derived protein GI 1890573 /xyloglucan endotransglycosylase (barley clone PM5 gene HVPM5)
203402-41-9
xyloglucanotransferase, xyloglucan (xyloglucan donor) (barley clone XEA gene HVXEA) /genBank X93174-derived protein GI 1890575 /xyloglucan X93174-derived protein GI 1890575 /xyloglucan endotransglycosylase (barley clone XEA gene HVXEA)
203402-42-0
xyloglucanotransferase, xyloglucan (xyloglucan donor) (barley clone XEB gene HVXEB) /genbank X93175-derived protein GI 1890577 /xyloglucan endotransglycosylase (barley clone XEB gene HVXEB)
205513-08-2
xyloglucanotransferase, xyloglucan (xyloglucan donor) (Actinia chinensis clone AdXET-5 gene XET precursor) /genBank L46792-derived protein GI 950299 /Xyloglucan endotransglycosylase (Actinia deliciosa strain Hayward pericarp clone AdXET-5 gene XET precursor)
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
kiwifruit xyloglucan + XXXG-ol
?
show the reaction diagram
-
-
-
?
tamarind xyloglucan + XXXG-ol
?
show the reaction diagram
-
-
-
?
donor xyloglucan + acceptor xyloglucan
?
show the reaction diagram
-
kiwifruit xyloglucan donor, acceptor: XXXG-ol, a reduced heptasaccharide derived from kiwifruit xyloglucan
-
-
?
donor xyloglucan + Xylalpha(1-6)Glcbeta(1-4)(Xylalpha(1-6))Glcbeta(1-4)(Xylalpha(1-6))Glcbeta(1-4)Glc-ol
?
show the reaction diagram
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XXXG-ol
-
-
?
donor xyloglucan + xyloglucan acceptor
?
show the reaction diagram
-
XET and xyloglucan may play a role in the cell wall changes that accompany fruit softening during ripening, substrates for XET action are located in the cell wall
-
-
?
kiwifruit xyloglucan + XXXG-ol
?
show the reaction diagram
-
-
-
?
tamarind xyloglucan + XXXG-ol
?
show the reaction diagram
-
-
-
?
additional information
?
-
-
in absence of reduced xyloglucan-derived heptasaccharide XXXG-ol enzyme depolymerizes xyloglucan by hydrolysis and in the presence of it by both hydrolysis and endotransglycosylation
-
-
?
NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
donor xyloglucan + xyloglucan acceptor
?
show the reaction diagram
-
XET and xyloglucan may play a role in the cell wall changes that accompany fruit softening during ripening, substrates for XET action are located in the cell wall
-
-
?
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
Hg2+
-
at least 10 mM
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.1
Xylalpha(1-6)Glcbeta(1-4)(Xylalpha(1-6))Glcbeta(1-4)(Xylalpha(1-6))Glcbeta(1-4)Glc-ol
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reduced xyloglucan-derived heptasaccharide XXXG-ol, derived from kiwifruit xyloglucan
additional information
additional information
-
Km: 0.6 mg/ml for kiwifruit xyloglucan
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SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
additional information
-
-
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
5.5 - 5.8
-
-
pH RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
5 - 8
-
active between
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
22
-
assay at
pI VALUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
8
calculated from amino acid sequence
6.1
calculated from amino acid sequence
6.4
calculated from amino acid sequence
6.7
calculated from amino acid sequence
7.5
calculated from amino acid sequence
8.3
calculated from amino acid sequence
9.2
calculated from amino acid sequence
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
-
UniProt
Manually annotated by BRENDA team
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
-
most of the XET activity is strongly associated with the cell wall
Manually annotated by BRENDA team
UNIPROT
ENTRY NAME
ORGANISM
NO. OF AA
NO. OF TRANSM. HELICES
MOLECULAR WEIGHT[Da]
SOURCE
SEQUENCE
LOCALIZATION PREDICTION?
C0IRG6_ACTDE
294
0
33175
TrEMBL
Secretory Pathway (Reliability: 1)
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
30200
calculated from amino acid sequence
29500
calculated from amino acid sequence
30200
calculated from amino acid sequence
31800
calculated from amino acid sequence
31900
calculated from amino acid sequence
34000
-
x * 34000, predicted MW of the mature protein encoded by AdXET5 is 31.8 kDa, SDS-PAGE
35500
calculated from amino acid sequence
SUBUNIT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
?
-
x * 34000, predicted MW of the mature protein encoded by AdXET5 is 31.8 kDa, SDS-PAGE
POSTTRANSLATIONAL MODIFICATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
glycoprotein
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AdXET5 and AdXET6: asparagine-linked carbohydrate, N-glycosylated, glycosylation of the protein is not required for endo-transglycosylase activity
proteolytic modification
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21 amino acids signal peptide at the N-terminus is cleaved to produce the mature protein
GENERAL STABILITY
ORGANISM
UNIPROT
LITERATURE
loss of activity if frozen and stored at -80°C
-
STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
-80°C, partially purified XET, ConA-eluate stage, 4 weeks, stable
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-80°C, purified XET, loss of activity
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4°C, purified XET, 24 h, stable
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PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
His-Trap chelating column chromatography
3000fold, AdXET6
-
His-Trap chelating column chromatography
CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
isoform XTH7 is expressed in Escherichia coli BL21 cells
6 isoforms, AdXET1-6 gene family is cloned, a full-length cDNA, AdXET5, is cloned, sequenced and overexpressed as active XET in Escherichia coli, purified XET is encoded by AdXET6
-
isoform XTH5 is expressed in Escherichia coli BL21 cells
REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Schrder, R.; Atkinson, R.G.; Langenkmper, G.; Redgwell, R.J.
Biochemical and molecular characterisation of xyloglucan endotransglycosylase from ripe kiwifruit
Planta
204
242-251
1998
Actinidia deliciosa
Manually annotated by BRENDA team
Atkinson, R.; Johnston, S.; Yauk, Y.; Sharma, N.; Schroeder, R.
Analysis of xyloglucan endotransglucosylase/hydrolase (XTH) gene families in kiwifruit and apple
Postharvest Biol. Technol.
51
149-157
2009
Actinidia eriantha (C0IRG0), Actinidia eriantha (C0IRG2), Actinidia eriantha (C0IRH1), Actinidia setosa (C0IRG1), Actinidia deliciosa (C0IRG3), Actinidia deliciosa (C0IRG4), Actinidia deliciosa (C0IRG5), Actinidia deliciosa (C0IRG6), Actinidia deliciosa (C0IRG7), Actinidia deliciosa (C0IRG9), Actinidia deliciosa (C0IRH2), Actinidia hemsleyana (C0IRG8), Actinidia chinensis (C0IRH0), Malus domestica (C0IRH4), Malus domestica (C0IRH6), Malus domestica (C0IRH7), Malus domestica (C0IRH8), Malus domestica (C0IRH9), Malus domestica (C0IRI0), Malus domestica (C0IRI1), Malus domestica (C0IRI2), Malus domestica (C0IRI3), Malus domestica (C0IRI4), Malus domestica (Q8GTJ0)
-
Manually annotated by BRENDA team