Information on EC 2.4.1.207 - xyloglucan:xyloglucosyl transferase

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The expected taxonomic range for this enzyme is: Streptophytina

EC NUMBER
COMMENTARY
2.4.1.207
-
RECOMMENDED NAME
GeneOntology No.
xyloglucan:xyloglucosyl transferase
REACTION
REACTION DIAGRAM
COMMENTARY
ORGANISM
UNIPROT ACCESSION NO.
LITERATURE
breaks a beta-(1->4) bond in the backbone of a xyloglucan and transfers the xyloglucanyl segment on to O-4 of the non-reducing terminal glucose residue of an acceptor, which can be a xyloglucan or an oligosaccharide of xyloglucan
show the reaction diagram
transglycosylic mechanism, i.e. random splitting of the beta-1,4-linked polyglucose backbone of xyloglucan molecules and rejoining the newly created reducing ends by beta-1,4 glycosidic bonds to nonreducing ends of other xyloglucan molecules or xyloglucan subunit oligosaccharides
-
breaks a beta-(1->4) bond in the backbone of a xyloglucan and transfers the xyloglucanyl segment on to O-4 of the non-reducing terminal glucose residue of an acceptor, which can be a xyloglucan or an oligosaccharide of xyloglucan
show the reaction diagram
sequential mechanism
-
breaks a beta-(1->4) bond in the backbone of a xyloglucan and transfers the xyloglucanyl segment on to O-4 of the non-reducing terminal glucose residue of an acceptor, which can be a xyloglucan or an oligosaccharide of xyloglucan
show the reaction diagram
ping-pong bi bi reaction mechanism; transglycosylic mechanism, i.e. random splitting of the beta-1,4-linked polyglucose backbone of xyloglucan molecules and rejoining the newly created reducing ends by beta-1,4 glycosidic bonds to nonreducing ends of other xyloglucan molecules or xyloglucan subunit oligosaccharides
-
breaks a beta-(1->4) bond in the backbone of a xyloglucan and transfers the xyloglucanyl segment on to O-4 of the non-reducing terminal glucose residue of an acceptor, which can be a xyloglucan or an oligosaccharide of xyloglucan
show the reaction diagram
reaction mechanism
-
breaks a beta-(1->4) bond in the backbone of a xyloglucan and transfers the xyloglucanyl segment on to O-4 of the non-reducing terminal glucose residue of an acceptor, which can be a xyloglucan or an oligosaccharide of xyloglucan
show the reaction diagram
double-displacement reaction mechanism
-
breaks a beta-(1->4) bond in the backbone of a xyloglucan and transfers the xyloglucanyl segment on to O-4 of the non-reducing terminal glucose residue of an acceptor, which can be a xyloglucan or an oligosaccharide of xyloglucan
show the reaction diagram
enzyme possesss the catalytic sequence motif EIDFE conserved in the glycosyl hydrolase family 16
-, Q6YDN9
breaks a beta-(1->4) bond in the backbone of a xyloglucan and transfers the xyloglucanyl segment on to O-4 of the non-reducing terminal glucose residue of an acceptor, which can be a xyloglucan or an oligosaccharide of xyloglucan
show the reaction diagram
the enzyme contains a catalytic sequence motif conserved in the glycosyl hydrolase family 16
-
breaks a beta-(1->4) bond in the backbone of a xyloglucan and transfers the xyloglucanyl segment on to O-4 of the non-reducing terminal glucose residue of an acceptor, which can be a xyloglucan or an oligosaccharide of xyloglucan
show the reaction diagram
substrate binding and catalytic mechanism, active site structure, structure-function study
-
breaks a beta-(1->4) bond in the backbone of a xyloglucan and transfers the xyloglucanyl segment on to O-4 of the non-reducing terminal glucose residue of an acceptor, which can be a xyloglucan or an oligosaccharide of xyloglucan
show the reaction diagram
determination of cleavage sites within the donor substrate of isozymes
-
breaks a beta-(1->4) bond in the backbone of a xyloglucan and transfers the xyloglucanyl segment on to O-4 of the non-reducing terminal glucose residue of an acceptor, which can be a xyloglucan or an oligosaccharide of xyloglucan
show the reaction diagram
-
-
-
-
REACTION TYPE
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
transglycosylation
Q1XD17, -
-
transglycosylation
Q40144, Q43527, Q43528, Q6RHX7, Q6RHX8, Q6RHX9, Q6RHY0, Q6RHY1, Q9FR51, Q9FZ05, Q9SDX0, Q9SLN9
-
transglycosylation
Q5JZX2, -
-
transglycosylation
Q9ZV40
-
transglycosylation
-
-
transglycosylation
-
-
transglycosylation
-
-
transglycosylation
-
-
endotransglycosylation, a xyloglucan chain is cleaved and transferred to another acceptor chain; idling reaction
-
SYSTEMATIC NAME
IUBMB Comments
xyloglucan:xyloglucan xyloglucanotransferase
Does not use cello-oligosaccharides as either donor or acceptor.
SYNONYMS
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
AtXTH12
Q9FKL9
-
AtXTH13
Q9FKL8
-
AtXTH17
O80803
-
AtXTH18
Q9M0D2
-
AtXTH19
Q9M0D1
-
AtXTH21
Q9ZV40
-
AtXTH24
P24806
-
BR1
Q9SDX0
isozyme
DcXTH1
Q1PCS6
-
DcXTH1
Dianthus caryophyllus Light Pink Barbara
Q1PCS6
-
-
DcXTH2
D7US89
-
DcXTH2
Dianthus caryophyllus Light Pink Barbara
D7US89
-
-
DcXTH3
D7URZ0
-
DcXTH3
Dianthus caryophyllus Light Pink Barbara
D7URZ0
-
-
DcXTH4
D7URZ1
-
DcXTH4
Dianthus caryophyllus Light Pink Barbara
D7URZ1
-
-
endo-transglycosylase
-
-
endo-xyloglucan transferase
-
-
-
-
endo-xyloglucanase
-
-
endoxyloglucan transferase
-
-
-
-
endoxyloglucan transferase
C0IRH0, C0IRH3
-
endoxyloglucan transferase
C0IRG3, C0IRG4, C0IRG5, C0IRG6, C0IRG7, C0IRG9, C0IRH2
-
endoxyloglucan transferase
C0IRG0, C0IRG2, C0IRH1
-
endoxyloglucan transferase
C0IRG8
-
endoxyloglucan transferase
C0IRG1
-
endoxyloglucan transferase
C0IRH4, C0IRH6, C0IRH7, C0IRH8, C0IRH9, C0IRI0, C0IRI1, C0IRI2, C0IRI3, C0IRI4, Q8GTJ0
-
EXGT
-
-
-
-
EXGT
C0IRH0, C0IRH3
-
EXGT
C0IRG3, C0IRG4, C0IRG5, C0IRG6, C0IRG7, C0IRG9, C0IRH2
-
EXGT
C0IRG0, C0IRG2, C0IRH1
-
EXGT
C0IRG1
-
EXGT
C0IRH4, C0IRH6, C0IRH7, C0IRH8, C0IRH9, C0IRI0, C0IRI1, C0IRI2, C0IRI3, C0IRI4, Q8GTJ0
-
EXT
-
-
-
-
GenBank L46792-derived protein GI 950299
-
-
-
-
GenBank X93173-derived protein GI 1890573
-
-
-
-
GenBank X93174-derived protein GI 1890575
-
-
-
-
Genbank X93175-derived protein GI 1890577
-
-
-
-
GenBank Z97335-derived protein GI
-
-
-
-
GhXTH1
-
gene name
GhXTH1
Gossypium hirsutum Coker 312
-
gene name
-
GhXTH2
-
gene name
GhXTH2
Gossypium hirsutum Coker 312
-
gene name
-
GhXTH3
-
gene name
group 3 XTH
-
-
HvXET3
P93671
-
HvXET4
P93672
-
HvXET6
B1P1S7
-
NXET
-
-
-
-
SkXTH1
Q1XD17
-
SlXTH1
Q40144
-
SlXTH10
Q43528
-
SlXTH11
Q43527
-
SlXTH12
Q9SDX0
-
SlXTH2
Q9FZ05
-
SlXTH3
Q6RHY0
-
SlXTH4
Q9FR51
-
SlXTH5
Q6RHY1
-
SlXTH6
Q6RHX9
-
SlXTH7
Q6RHX8
-
SlXTH8
Q9SLN9
-
SlXTH9
Q6RHX7
-
XET
-
-
-
-
XET
C0IRH0, C0IRH3
-
XET
C0IRG3, C0IRG4, C0IRG5, C0IRG6, C0IRG7, C0IRG9, C0IRH2
-
XET
C0IRG0, C0IRG2, C0IRH1
-
XET
C0IRG1
-
XET
Q7Y252
-
XET
Gossypium hirsutum Coker 312
-
-
-
XET
B1P1S7
-
XET
P93668
-
XET
P93671, P93672
-
XET
C0IRH4, C0IRH6, C0IRH7, C0IRH8, C0IRH9, C0IRI0, C0IRI1, C0IRI2, C0IRI3, C0IRI4, Q8GTJ0
-
XET
P93349
-
XET
Q43527, Q43528
-
XET
Q6RHX7, Q6RHX8, Q6RHX9, Q6RHY0, Q6RHY1, Q9FR51, Q9FZ05, Q9SDX0, Q9SLN9
-
XET(6.3)
-
major enzyme form, XET(6.3) is classified into group II of the XTH phylogeny of glycoside hydrolase family GH16
XET3
P93671
isozyme
XET4
P93672
isozyme
XET5
P93668
isozyme
XET6
B1P1S7
isozyme
XTH
-
-
-
XTH
O80803, Q9FKL8, Q9FKL9, Q9M0D1, Q9M0D2
-
XTH
O65734
-
XTH
D7URZ0, D7URZ1, D7US89, Q1PCS6
-
XTH
Dianthus caryophyllus Light Pink Barbara
D7URZ0, D7URZ1, D7US89, Q1PCS6
-
-
XTH
Gossypium hirsutum Coker 312
-
-
-
XTH
B1P1S7, P93671, P93672
-
XTH
-, Q5Z6G7, Q5Z6H1, Q5Z6H3, Q6ZAN9, Q763W5, Q76BW5, Q8VWG5
-
XTH
Q40144, Q43527, Q43528, Q6RHX7, Q6RHX8, Q6RHX9, Q6RHY0, Q6RHY1, Q9FR51, Q9FZ05, Q9SDX0, Q9SLN9
-
XTH/XET
Allium ampeloprasum, Cucumis sativus, Daucus carota, Lactuca sativa ssp. capitata
-
-
XTH/XET
-
-
XTH/XET
Piper sp., Pisum sativum
-
-
XTH1
C0IRG0
isozyme
XTH1
-
isozyme
XTH1
Q7Y252
isozyme
XTH1
-
isozyme
XTH1
C0IRH4
isozyme
XTH1
Q40144
isozyme
XTH1
-
shows both transglycosylase and hydrolase activities when assayed in vitro (depending on the substrates), however, in planta the enzyme acts only as a hydrolase to degrade storage xyloglucan
XTH1
Q5MB21
-
XTH10
C0IRG9
isozyme
XTH10
C0IRI3
isozyme
XTH11
C0IRH0
isozyme
XTH11
C0IRI4
isozyme
XTH12
C0IRH1
isozyme
XTH12
Q9SDX0
isozyme
XTH13
C0IRH2
isozyme
XTH14
C0IRH3
isozyme
XTH14
-
isozyme
XTH2
C0IRG1
isozyme
XTH2
Q8GTJ0
isozyme
XTH2
Q9FZ05
isozyme
XTH24
P24806
-
XTH26
-
isozyme
XTH28
Q38909
-
XTH3
C0IRG2
isozyme
XTH3
C0IRH6
isozyme
XTH3
Q6RHY0
isozyme
XTH4
C0IRG3
isozyme
XTH4
C0IRH7
isozyme
XTH4
Q9FR51
isozyme
XTH5
C0IRG4
isozyme
XTH5
C0IRH8
isozyme
XTH5
Q6RHY1
isozyme
XTH6
C0IRG5
isozyme
XTH6
C0IRH9
isozyme
XTH6
Q6RHX9
isozyme
XTH7
C0IRG6
isozyme
XTH7
C0IRI0
isozyme
XTH7
Q6RHX8
isozyme
XTH8
C0IRG7
isozyme
XTH8
C0IRI1
isozyme
XTH8
Q9SLN9
isozyme
XTH9
C0IRG8
isozyme
XTH9
C0IRI2
isozyme
XTH9
Q6RHX7
isozyme
xyloglucan endo-transglucosylase
-
-
xyloglucan endo-transglycosylase
-
-
xyloglucan endo-transglycosylase
Q8GZD5
-
xyloglucan endo-transglycosylase
-
-
xyloglucan endo-transglycosylase/xyloglucan endo-transglycosylase/hydrolase
Q8GZD5
-
xyloglucan endo-transglycosylases/hydrolase
-
-
xyloglucan endotransglucosylase
P24806
-
xyloglucan endotransglucosylase
-
-
xyloglucan endotransglucosylase
-
-
xyloglucan endotransglucosylase
-
-
xyloglucan endotransglucosylase
P93349
-
xyloglucan endotransglucosylase
-
-
xyloglucan endotransglucosylase
Q1XD17
-
xyloglucan endotransglucosylase
Q40144
-
xyloglucan endotransglucosylase
Q43527, Q43528
-
xyloglucan endotransglucosylase
Q6RHX7, Q6RHX8, Q6RHX9, Q6RHY0, Q6RHY1, Q9FR51, Q9FZ05, Q9SDX0, Q9SLN9
-
xyloglucan endotransglucosylase
-
-
xyloglucan endotransglucosylase 16A
Q8GZD5
-
xyloglucan endotransglucosylase/endohydrolase
Q1XD17
-
xyloglucan endotransglucosylase/hydrolase
C0IRH0, C0IRH3
-
xyloglucan endotransglucosylase/hydrolase
C0IRG3, C0IRG4, C0IRG5, C0IRG6, C0IRG7, C0IRG9, C0IRH2
-
xyloglucan endotransglucosylase/hydrolase
C0IRG0, C0IRG2, C0IRH1
-
xyloglucan endotransglucosylase/hydrolase
C0IRG8
-
xyloglucan endotransglucosylase/hydrolase
C0IRG1
-
xyloglucan endotransglucosylase/hydrolase
-
-
xyloglucan endotransglucosylase/hydrolase
O80803
-
xyloglucan endotransglucosylase/hydrolase
Q38909
-
xyloglucan endotransglucosylase/hydrolase
Q9FKL8, Q9FKL9, Q9M0D1, Q9M0D2
-
xyloglucan endotransglucosylase/hydrolase
Q9ZV40
-
xyloglucan endotransglucosylase/hydrolase
-
-
xyloglucan endotransglucosylase/hydrolase
-
-
xyloglucan endotransglucosylase/hydrolase
O65734
-
xyloglucan endotransglucosylase/hydrolase
D7URZ0, D7URZ1, D7US89, Q1PCS6
-
xyloglucan endotransglucosylase/hydrolase
Dianthus caryophyllus Light Pink Barbara
D7URZ0, D7URZ1, D7US89, Q1PCS6
-
-
xyloglucan endotransglucosylase/hydrolase
C0IRH4, C0IRH6, C0IRH7, C0IRH8, C0IRH9, C0IRI0, C0IRI1, C0IRI2, C0IRI3, C0IRI4, Q8GTJ0
-
xyloglucan endotransglucosylase/hydrolase
-
-
xyloglucan endotransglucosylase/hydrolase
-, Q5Z6G7, Q5Z6H1, Q5Z6H3, Q6ZAN9, Q763W5, Q76BW5, Q8VWG5
-
xyloglucan endotransglucosylase/hydrolase
-
-
xyloglucan endotransglucosylase/hydrolase
-
-
xyloglucan endotransglucosylase/hydrolase
Q40144
-
xyloglucan endotransglucosylase/hydrolase
Q43527, Q43528
-
xyloglucan endotransglucosylase/hydrolase
Q6RHX7, Q6RHX8, Q6RHX9, Q6RHY0, Q6RHY1, Q9FR51, Q9FZ05, Q9SDX0, Q9SLN9
-
xyloglucan endotransglucosylase/hydrolase
-
-
xyloglucan endotransglucosylase/hydrolase
-
-
xyloglucan endotransglycosylase
-
-
-
-
xyloglucan endotransglycosylase
C0IRH0, C0IRH3
-
xyloglucan endotransglycosylase
C0IRG3, C0IRG4, C0IRG5, C0IRG6, C0IRG7, C0IRG9, C0IRH2
-
xyloglucan endotransglycosylase
C0IRG0, C0IRG2, C0IRH1
-
xyloglucan endotransglycosylase
C0IRG8
-
xyloglucan endotransglycosylase
C0IRG1
-
xyloglucan endotransglycosylase
-
-
xyloglucan endotransglycosylase
-
-
xyloglucan endotransglycosylase
-
-
xyloglucan endotransglycosylase
-
-
xyloglucan endotransglycosylase
Q7Y252
-
xyloglucan endotransglycosylase
-
-
xyloglucan endotransglycosylase
B1P1S7
-
xyloglucan endotransglycosylase
P93668
-
xyloglucan endotransglycosylase
P93671, P93672
-
xyloglucan endotransglycosylase
C0IRH4, C0IRH6, C0IRH7, C0IRH8, C0IRH9, C0IRI0, C0IRI1, C0IRI2, C0IRI3, C0IRI4, Q8GTJ0
-
xyloglucan endotransglycosylase
-
-
xyloglucan endotransglycosylase
-
-
xyloglucan endotransglycosylase (Actinia deliciosa strain Hayward pericarp clone AdXET-5 gene XET precursor)
-
-
-
-
xyloglucan endotransglycosylase (Arabidopsis thaliana gene TCH4 precursor reduced)
-
-
-
-
xyloglucan endotransglycosylase (barley clone PM5 gene HVPM5)
-
-
-
-
xyloglucan endotransglycosylase (barley clone XEA gene HVXEA)
-
-
-
-
xyloglucan endotransglycosylase (barley clone XEB gene HVXEB)
-
-
-
-
xyloglucan endotransglycosylase 16A
Q6YDN9
-
xyloglucan endotransglycosylase 16A
-
-
xyloglucan endotransglycosylase-related protein XTR-7 (Arabidopsis thaliana)
-
-
-
-
xyloglucan endotransglycosylase/hydrolase
-
-
xyloglucan endotransglycosylase/hydrolase
-
-
xyloglucan endotransglycosylase/hydrolase
-
-
xyloglucan endotransglycosylase/hydrolase
-
-
xyloglucan endotransglycosylase/hydrolase
Gossypium hirsutum Coker 312
-
-
-
xyloglucan endotransglycosylase/hydrolase
B1P1S7, P93671, P93672
-
xyloglucan endotransglycosylase/hydrolase
Lactuca sativa ssp. capitata, Piper sp., Pisum sativum
-
-
xyloglucan endotransglycosylase/hydrolase
-
-
xyloglucan endotransglycosylase/hydrolase
-
-
xyloglucan endotransglycosylase/hydrolase
-
-
xyloglucan endotransglycosylase/hydrolase1
Q5MB21
-
xyloglucan endotransglycosylation activity
-
-
xyloglucan hydrolase
-
-
xyloglucan recombinase
-
-
-
-
xyloglucan X93174-derived protein GI 1890575
-
-
-
-
xyloglucan xyloglucosyl transferase
-
-
xyloglucan xyloglucosyl transferase
B1P1S7
-
xyloglucan xyloglucosyl transferase
P93668
-
xyloglucan xyloglucosyl transferase
P93671, P93672
-
xyloglucan xyloglucosyl transferases
B1P1S7, P93671, P93672
-
xyloglucan-specific endo-(1->4)-beta-D-glucanase
-
-
-
-
xyloglucan:xyloglucanotransferase
-
-
-
-
xyloglucanase 1
-
-
xyloglucanotransferase, xyloglucan (xyloglucan donor)
-
-
-
-
xyloglucanotransferase, xyloglucan (xyloglucan donor) (Actinia chinensis clone AdXET-5 gene XET precursor)
-
-
-
-
xyloglucanotransferase, xyloglucan (xyloglucan donor) (Arabidopsis thaliana gene TCH4 precursor reduced)
-
-
-
-
xyloglucanotransferase, xyloglucan (xyloglucan donor) (barley clone PM5 gene HVPM5)
-
-
-
-
xyloglucanotransferase, xyloglucan (xyloglucan donor) (barley clone XEA gene HVXEA)
-
-
-
-
xyloglucanotransferase, xyloglucan (xyloglucan donor) (barley clone XEB gene HVXEB)
-
-
-
-
ZmXTH1
-
in addition to ZmXTH1 and Zm1005, six other XTH genes are identified in maize EST databases
additional information
Q6YDN9
the enzyme belongs to the glycosyl hydrolase family 16, i.e. GH16
additional information
-, Q5Z6G7, Q5Z6H1, Q5Z6H3, Q6ZAN9, Q763W5, Q76BW5, Q8VWG5
the enzyme belongs to the 29-member xyloglucan endotransglucosylase/hydrolase XTH gene family
additional information
-
the enzyme belongs to the glycosyl hydrolase family 16, i.e. GH16
additional information
Q8GZD5
the enzyme belongs to the glycosyl hydrolase family 16, i.e. GH16
CAS REGISTRY NUMBER
COMMENTARY
141588-40-1
-
172279-31-1
xyloglucanotransferase, xyloglucan (xyloglucan donor) (Arabidopsis thaliana gene TCH4 precursor reduced) /xyloglucan endotransglycosylase (Arabidopsis thaliana gene TCH4 precursor reduced)
202012-34-8
xyloglucan endotransglycosylase-related protein XTR-7 (Arabidopsis thaliana) /genBank Z97335-derived protein GI 2244769
203402-40-8
xyloglucanotransferase, xyloglucan (xyloglucan donor) (barley clone PM5 gene HVPM5) /genBank X93173-derived protein GI 1890573 /xyloglucan endotransglycosylase (barley clone PM5 gene HVPM5)
203402-41-9
xyloglucanotransferase, xyloglucan (xyloglucan donor) (barley clone XEA gene HVXEA) /genBank X93174-derived protein GI 1890575 /xyloglucan X93174-derived protein GI 1890575 /xyloglucan endotransglycosylase (barley clone XEA gene HVXEA)
203402-42-0
xyloglucanotransferase, xyloglucan (xyloglucan donor) (barley clone XEB gene HVXEB) /genbank X93175-derived protein GI 1890577 /xyloglucan endotransglycosylase (barley clone XEB gene HVXEB)
205513-08-2
xyloglucanotransferase, xyloglucan (xyloglucan donor) (Actinia chinensis clone AdXET-5 gene XET precursor) /genBank L46792-derived protein GI 950299 /Xyloglucan endotransglycosylase (Actinia deliciosa strain Hayward pericarp clone AdXET-5 gene XET precursor)
ORGANISM
COMMENTARY
LITERATURE
SEQUENCE CODE
SEQUENCE DB
SOURCE
ripe kiwifruit, [A. Chev.] C.F. Liang et A.R. Ferguson var. deliciosa cv. Hayward, 6 isoenzymes: AdXET1-6 gene family
-
-
Manually annotated by BRENDA team
fragment
UniProt
Manually annotated by BRENDA team
Adiantum capillus-veneri
-
-
-
Manually annotated by BRENDA team
leek, at least 4 isozymes
-
-
Manually annotated by BRENDA team
Aloe sp.
-
-
-
Manually annotated by BRENDA team
Anthurium upalaense
-
-
-
Manually annotated by BRENDA team
Apium sp.
-
-
-
Manually annotated by BRENDA team
Apium sp.
celery
-
-
Manually annotated by BRENDA team
3 insect-cell-produced XETs: EXGT, TCH4 and MERI-5, EXGT belongs to XET class I, TCH4 and MERI-5 to XET class II
-
-
Manually annotated by BRENDA team
ecotype Columbia
-
-
Manually annotated by BRENDA team
ecotype Columbia; systematic nomenclature of the XTH gene family with 3 subfamilies
-
-
Manually annotated by BRENDA team
ecotype Columbia; TCH4, member of the XET-related gene family
-
-
Manually annotated by BRENDA team
isozyme AtXTH12; 33 XTH genes
UniProt
Manually annotated by BRENDA team
isozyme AtXTH13; 33 XTH genes
UniProt
Manually annotated by BRENDA team
isozyme AtXTH17; 33 XTH genes
UniProt
Manually annotated by BRENDA team
isozyme AtXTH18; 33 XTH genes
UniProt
Manually annotated by BRENDA team
isozyme AtXTH19; 33 XTH genes
UniProt
Manually annotated by BRENDA team
TCH4, member of the XET-related gene family
-
-
Manually annotated by BRENDA team
XETs are encoded by a gene family, 4 isoenzymes: TCH4, Meri-5, EXGT and XTR9
-
-
Manually annotated by BRENDA team
XTR9, one of 4 isoenzymes: TCH4, Meri-5, EXGT and XTR9
SwissProt
Manually annotated by BRENDA team
Asphodelus fistulosus
-
-
-
Manually annotated by BRENDA team
Azolla sp.
-
-
-
Manually annotated by BRENDA team
Beckmannia syzigachne Fernald
Fernald
-
-
Manually annotated by BRENDA team
Blumenbachia hieronymi
-
-
-
Manually annotated by BRENDA team
cauliflower
-
-
Manually annotated by BRENDA team
cauliflower; distinct isoforms
-
-
Manually annotated by BRENDA team
cauliflower; isoenzyme C45a
-
-
Manually annotated by BRENDA team
cauliflower; var. botrytis, 2 isoenzymes: C30, C45
-
-
Manually annotated by BRENDA team
Calandrinia grandiflora
-
-
-
Manually annotated by BRENDA team
subsp. Flacca
-
-
Manually annotated by BRENDA team
Cedrus atlantica Carr.
Carr.
-
-
Manually annotated by BRENDA team
Chamaecyparis sp.
Snow White
-
-
Manually annotated by BRENDA team
chickpea
-
-
Manually annotated by BRENDA team
Commelina nilagirica
-
-
-
Manually annotated by BRENDA team
Crocus vernus Hill
Hill
-
-
Manually annotated by BRENDA team
at least 4 isozymes
-
-
Manually annotated by BRENDA team
no isozymes, single enzyme
-
-
Manually annotated by BRENDA team
DcXTH1; four XTH genes DcXTH1-DcXTH4
UniProt
Manually annotated by BRENDA team
DcXTH2; four XTH genes DcXTH1-DcXTH4
UniProt
Manually annotated by BRENDA team
DcXTH3; four XTH genes DcXTH1-DcXTH4
UniProt
Manually annotated by BRENDA team
DcXTH4; four XTH genes DcXTH1-DcXTH4
UniProt
Manually annotated by BRENDA team
Dianthus caryophyllus L.
-
-
Manually annotated by BRENDA team
Dianthus caryophyllus Light Pink Barbara
DcXTH1; four XTH genes DcXTH1-DcXTH4
UniProt
Manually annotated by BRENDA team
Dianthus caryophyllus Light Pink Barbara
DcXTH2; four XTH genes DcXTH1-DcXTH4
UniProt
Manually annotated by BRENDA team
Dianthus caryophyllus Light Pink Barbara
DcXTH3; four XTH genes DcXTH1-DcXTH4
UniProt
Manually annotated by BRENDA team
Dianthus caryophyllus Light Pink Barbara
DcXTH4; four XTH genes DcXTH1-DcXTH4
UniProt
Manually annotated by BRENDA team
variant 79/BH47
-
-
Manually annotated by BRENDA team
cultivars Acala SJ2, Palmeri, 138F, and PI529989
UniProt
Manually annotated by BRENDA team
isozymes GhXTH1, GhXTH2 and GhXTH3
-
-
Manually annotated by BRENDA team
Gossypium hirsutum Coker 312
isozymes GhXTH1, GhXTH2 and GhXTH3
-
-
Manually annotated by BRENDA team
variant Ulbrich
-
-
Manually annotated by BRENDA team
cultivar Clipper
-
-
Manually annotated by BRENDA team
HvXET3
UniProt
Manually annotated by BRENDA team
HvXET4
UniProt
Manually annotated by BRENDA team
HvXET6
SwissProt
Manually annotated by BRENDA team
isozyme XET6
SwissProt
Manually annotated by BRENDA team
Lactuca sativa ssp. capitata
cabbage, no isozymes, single enzyme
-
-
Manually annotated by BRENDA team
cultivar Royal Gala
UniProt
Manually annotated by BRENDA team
isozyme XTH10; cultivar Royal Gala
C0IRI3
UniProt
Manually annotated by BRENDA team
isozyme XTH11; cultivar Royal Gala
UniProt
Manually annotated by BRENDA team
isozyme XTH2; cultivar Braeburn
UniProt
Manually annotated by BRENDA team
isozyme XTH3; cultivar Royal Gala
UniProt
Manually annotated by BRENDA team
isozyme XTH4; cultivar Royal Gala
UniProt
Manually annotated by BRENDA team
isozyme XTH5; cultivar Royal Gala
UniProt
Manually annotated by BRENDA team
isozyme XTH6; cultivar Braeburn
UniProt
Manually annotated by BRENDA team
isozyme XTH7; cultivar Royal Gala
UniProt
Manually annotated by BRENDA team
isozyme XTH8; cultivar M.9
C0IRI1
UniProt
Manually annotated by BRENDA team
isozyme XTH9; cultivar Royal Gala
UniProt
Manually annotated by BRENDA team
cv. Samsun NN, NtXET-1, belongs to group I of XET-related subfamilies
SwissProt
Manually annotated by BRENDA team
tobacco bright yellow-2
-
-
Manually annotated by BRENDA team
Nicotiana tabacum SR-1
SR-1
-
-
Manually annotated by BRENDA team
isozyme XTH1 and XTH21; isozymes XTH1 and XTH21
SwissProt
Manually annotated by BRENDA team
isozyme XTH25; isozyme XTH25
Q8VWG5
SwissProt
Manually annotated by BRENDA team
isozyme XTH5; isozyme XTH5
SwissProt
Manually annotated by BRENDA team
isozymes XTH10, XTH11, XTH12, and XTH18; isozymes XTH10, XTH11, XTH12, and XTH18
SwissProt
Manually annotated by BRENDA team
isozymes XTH2-XTH4, XTH6, XTH7, XTH9, XTH13-XTH16, XTH19, XTH20, and XTH22-XTH29
-
-
Manually annotated by BRENDA team
isozymes XTH8 and XTH17; isozymes XTH8 and XTH17
SwissProt
Manually annotated by BRENDA team
Pharbitis purpurea
Bojer
-
-
Manually annotated by BRENDA team
Pharbitis purpurea Bojer
Bojer
-
-
Manually annotated by BRENDA team
cv. Canadian Wonder
-
-
Manually annotated by BRENDA team
Phaseolus vulgaris L.
-
-
Manually annotated by BRENDA team
Pinus parviflora Glauca
Glauca
-
-
Manually annotated by BRENDA team
Piper sp.
green pepper, no isozymes, single enzyme
-
-
Manually annotated by BRENDA team
cv. Alaska
-
-
Manually annotated by BRENDA team
cv. Tyrkys
-
-
Manually annotated by BRENDA team
var. Feltham First and Pilot
-
-
Manually annotated by BRENDA team
var. Tyrkys, no isozymes, single enzyme
-
-
Manually annotated by BRENDA team
Pitcairnia imbricata
Regel
-
-
Manually annotated by BRENDA team
tremuloides
-
-
Manually annotated by BRENDA team
hybrid aspen, poplar, major isoenzyme PttXET16A, a member of XET subfamily I
SwissProt
Manually annotated by BRENDA team
Pothomorphe petalta
-
-
-
Manually annotated by BRENDA team
arrowhead
-
-
Manually annotated by BRENDA team
Selaginella sp.
-
-
-
Manually annotated by BRENDA team
Sinapis sp.
mustard
-
-
Manually annotated by BRENDA team
cultivar Ailsa Craig, formerly Lycopersicon esculentum
-
-
Manually annotated by BRENDA team
cultivar Money Maker
SwissProt
Manually annotated by BRENDA team
cultivar Money Maker
UniProt
Manually annotated by BRENDA team
cultivar Money Maker; isozyme BR1; cultivar Money Maker
UniProt
Manually annotated by BRENDA team
cultivar Money Maker; isozyme XTH9; cultivar Money Maker
UniProt
Manually annotated by BRENDA team
cv. Ailsa Craig
-
-
Manually annotated by BRENDA team
cv. Murrieta
-
-
Manually annotated by BRENDA team
isozyme XTH7; cultivar Money Maker
UniProt
Manually annotated by BRENDA team
SlXTH1, XTH1; Solanum lycopersicum L. cv. Money Maker, Solanum lycopersicum cv. Canario
SwissProt
Manually annotated by BRENDA team
SlXTH10, tXET-B2, LetXET-B2; Solanum lycopersicum L. cv. Money Maker, Solanum lycopersicum cv. Canario
UniProt
Manually annotated by BRENDA team
SlXTH11, tXET-B1, LetXET-B1; Solanum lycopersicum L. cv. Money Maker, Solanum lycopersicum cv. Canario
UniProt
Manually annotated by BRENDA team
SlXTH12, BR1 fragment, LeBR1; Solanum lycopersicum L. cv. Money Maker, Solanum lycopersicum cv. Canario
UniProt
Manually annotated by BRENDA team
SlXTH2, LeXET2; Solanum lycopersicum L. cv. Money Maker, Solanum lycopersicum cv. Canario
UniProt
Manually annotated by BRENDA team
SlXTH3, XTH3; Solanum lycopersicum L. cv. Money Maker, Solanum lycopersicum cv. Canario
UniProt
Manually annotated by BRENDA team
SlXTH4, XET4; Solanum lycopersicum L. cv. Money Maker, Solanum lycopersicum cv. Canario
UniProt
Manually annotated by BRENDA team
SlXTH5, XTH5; Solanum lycopersicum L. cv. Money Maker, Solanum lycopersicum cv. Canario
UniProt
Manually annotated by BRENDA team
SlXTH6, XTH6; Solanum lycopersicum L. cv. Money Maker, Solanum lycopersicum cv. Canario
UniProt
Manually annotated by BRENDA team
SlXTH7, XTH7; Solanum lycopersicum L. cv. Money Maker, Solanum lycopersicum cv. Canario
UniProt
Manually annotated by BRENDA team
SlXTH8, ETAG-A3 fragment; Solanum lycopersicum L. cv. Money Maker, Solanum lycopersicum cv. Canario
UniProt
Manually annotated by BRENDA team
SlXTH9, XTH9; Solanum lycopersicum L. cv. Money Maker, Solanum lycopersicum cv. Canario
UniProt
Manually annotated by BRENDA team
Tragopodon pratensis
subs. Pratensis
-
-
Manually annotated by BRENDA team
Trigonella caerulea Ser.
Ser.
-
-
Manually annotated by BRENDA team
2 isoenzymes, one of which is seed specific
-
-
Manually annotated by BRENDA team
2 isoenzymes: sXET and eXET
-
-
Manually annotated by BRENDA team
cv. Goldshine orange
-
-
Manually annotated by BRENDA team
NXG1 is a member of XET class III
-
-
Manually annotated by BRENDA team
var. Fiery Festival, 2 enzyme forms encoded by the genes XET1 and NXG1
-
-
Manually annotated by BRENDA team
var. Goldshine orange, at least 5 isozymes
-
-
Manually annotated by BRENDA team
azuki bean
-
-
Manually annotated by BRENDA team
Ohwi and Ohashi, cv. Takara
-
-
Manually annotated by BRENDA team
mungbean
SwissProt
Manually annotated by BRENDA team
4 isoenzymes: M35, M45, M55a, M55b
-
-
Manually annotated by BRENDA team
4 isoenzymes: M35, M45, M55a, M55b; mung bean; Vigna radiata
-
-
Manually annotated by BRENDA team
distinct isoforms; mung bean
-
-
Manually annotated by BRENDA team
C.C. Gmel
-
-
Manually annotated by BRENDA team
-
SwissProt
Manually annotated by BRENDA team
GENERAL INFORMATION
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
evolution
-
the enzyme belongs to the GH16 XTH family, extension clan GH-B enzymes
physiological function
-
xyloglucan endo-transglucosylase can link different polysaccharides in vivo and hence influence cell wall strength, flexibility, and porosity
physiological function
Q38909
XTH28 is specifically involved in the growth of stamen fi laments, and is required for successful automatic self-pollination in certain flowers in Arabidopsis thaliana
physiological function
O80803, Q9FKL8, Q9FKL9, Q9M0D1, Q9M0D2
xyloglucan endotransglucosylase/hydrolases are cell wall enzymes that are able to graft xyloglucan chains to oligosaccharides or to other available xyloglucan chains and/or to hydrolyse xyloglucan chains. As they are involved in the modification of the load-bearing cell-wall components, they are believed to be very important in the regulation of growth and development; xyloglucan endotransglucosylase/hydrolases are cell wall enzymes that are able to graft xyloglucan chains to oligosaccharides or to other available xyloglucan chains and/or to hydrolyse xyloglucan chains. As they are involved in the modification of the load-bearing cell-wall components, they are believed to be very important in the regulation of growth and development; xyloglucan endotransglucosylase/hydrolases are cell wall enzymes that are able to graft xyloglucan chains to oligosaccharides or to other available xyloglucan chains and/or to hydrolyse xyloglucan chains. As they are involved in the modification of the load-bearing cell-wall components, they are believed to be very important in the regulation of growth and development. AtXTH13 might play a role later in root hair development; xyloglucan endotransglucosylase/hydrolases are cell wall enzymes that are able to graft xyloglucan chains to oligosaccharides or to other available xyloglucan chains and/or to hydrolyse xyloglucan chains. As they are involved in the modification of the load-bearing cell-wall components, they are believed to be very important in the regulation of growth and development. Role for AtXTH12 in root hair initiation. AtXTH13 might play a role later in root hair development; xyloglucan endotransglucosylase/hydrolases are cell wall enzymes that are able to graft xyloglucan chains to oligosaccharides or to other available xyloglucan chains and/or to hydrolyse xyloglucan chains. As they are involved in the modification of the load-bearing cell-wall components, they are believed to be very important in the regulation of growth and development. Role for AtXTH12 in root hair initiation. AtXTH13 might play a role later in root hair development
physiological function
D7URZ0, D7URZ1, D7US89, -, Q1PCS6
DcXTH2 is associated with petal growth and development during carnation flower opening; DcXTH3 is associated with petal growth and development during carnation flower opening
physiological function
B1P1S7, -, P93671, P93672
xyloglucan xyloglucosyl transferases modulate molecular masses of xyloglucans, size modulations and transfer reactions occur with polymeric acceptor substrates; xyloglucan xyloglucosyl transferases modulate molecular masses of xyloglucans, size modulations and transfer reactions occur with polymeric acceptor substrates; xyloglucan xyloglucosyl transferases modulate molecular masses of xyloglucans, size modulations and transfer reactions occur with polymeric acceptor substrates
physiological function
-
xyloglucan endotransglycosylase/hydrolases, XTH, are believed to play an important role in modifying the cell wall structure through two different, but related actions: the cleavage of a cross-linking xyloglucan polymer, xyloglucan endohydrolase or XEH activity, and transfer of a newly generated end to another sugar polymer, xyloglucan endotransglycosylase, XET, activity
physiological function
-
xyloglucan endo-transglycosylases, XETs, encoded by xyloglucan endo-transglycosylases/hydrolase, XTH, genes modify the xyloglucan-cellulose framework of plant cell walls, thereby regulating their expansion and strength. Importance of XET in wood development, xyloglucan dynamics and XTH gene expression in developing wood, overview. Several XTH genes are involved in the development of secondary vascular tissues, XET activity stimulates cell expansion in vessel elements but not in fibers. In young xylem cells, XET activity limits xyloglucan incorporation into the tightly bound wall network but removes it from cell walls in older cells. Importance of XET activity for vascular tissue differentiation and/or function. Increased XET activity has differing effects on the xyloglucan content of expanding and maturing wood cells
physiological function
-
plant cell wall extensibility is mediated, in part, by xyloglucan endotransglycosylases/hydrolases, XTH, that are able to cleave and reattach xyloglucan polymers that make up the hemicelluloses matrix of type I cell walls. The three GhXTH genes are expressed differently with GhXTH1 predominantly expressed in elongating cotton fibers, function of GhXTH1 in mediating cotton fiber elongation, the 35S::GhXTH1 transgene acts as a dominant fiber length allele in transgenic cotton, overview
physiological function
Dianthus caryophyllus Light Pink Barbara
-
DcXTH2 is associated with petal growth and development during carnation flower opening; DcXTH3 is associated with petal growth and development during carnation flower opening
-
physiological function
Gossypium hirsutum Coker 312
-
plant cell wall extensibility is mediated, in part, by xyloglucan endotransglycosylases/hydrolases, XTH, that are able to cleave and reattach xyloglucan polymers that make up the hemicelluloses matrix of type I cell walls. The three GhXTH genes are expressed differently with GhXTH1 predominantly expressed in elongating cotton fibers, function of GhXTH1 in mediating cotton fiber elongation, the 35S::GhXTH1 transgene acts as a dominant fiber length allele in transgenic cotton, overview
-
evolution
-
the enzyme belongs to the GH16 XTH family, gene models, overview
additional information
-
key structural features controlling the activity may involve the loop 1 and 2 regions of the XTH enzymes, homology modeling, detailed overview. The homology models of all 40 poplar XTH proteins is mainly focused on the areas near loop 1 and loop 2
additional information
-
catalytic nucleophile is Glu85, general acid/base residue is Glu89, Asp87 is the so-called helper residue, molecular dynamics simulations of a branched tetradecasaccharide substrate XXXGXXXG in the active site of xyloglucan endo-transglycosylase. When Asp87 is deprotonated, electrostatic repulsion forces the nucleophilic away from C1 of the sugar ring in subsite 21 and the proton-donating ability of Glu89 iss also weakened due to the formation of a hydrogen bond with Asp87, whereas protonation of Asp87 results in the formation of a hydrogen bond with the catalytic nucleophile and correct positioning of the catalytic machinery
additional information
-
transgenic up-regulation of XET activity induced changes in cell wall xyloglucan, but its effects are dependent on developmental stage. For instance, XET overexpression increases abundance of the CCRC-M1 epitope in cambial cells and xylem cells in early stages of differentiation but not in mature xylem. Correspondingly, an increase in tightly bound xyloglucan content is observed in primary-walled xylem but a decrease is seen in secondary-walled xylem
additional information
-
Plants that overexpress GhXTH1 have increased XTH activity and produce mature cotton fibers that are between 15 and 20% longer than wild-type cotton plants under both greenhouse and field growth conditions
additional information
Gossypium hirsutum Coker 312
-
Plants that overexpress GhXTH1 have increased XTH activity and produce mature cotton fibers that are between 15 and 20% longer than wild-type cotton plants under both greenhouse and field growth conditions
-
SUBSTRATE
PRODUCT                      
REACTION DIAGRAM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
(Substrate)
LITERATURE
(Substrate)
COMMENTARY
(Product)
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
donor barley (1,3,1,4)-beta-D-glucan + acceptor cellooligosaccharide
?
show the reaction diagram
-
0.1% relative activity compared to the reaction with donor tamarind xyloglucan and acceptor xyloglucan oligosaccharide
-
-
?
donor barley (1,3,1,4)-beta-D-glucan + acceptor xyloglucan oligosaccharide
?
show the reaction diagram
-
0.2% relative activity compared to the reaction with donor tamarind xyloglucan and acceptor xyloglucan oligosaccharide
-
-
?
donor carboxymethylcellulose + acceptor xyloglucan oligosaccharide
?
show the reaction diagram
-
0.4% relative activity compared to the reaction with donor tamarind xyloglucan and acceptor xyloglucan oligosaccharide
-
-
?
donor hydroxyethyl cellulose + acceptor xyloglucan
?
show the reaction diagram
-
-
-
-
?
donor hydroxyethylcellulose + acceptor cellooligosaccharide
?
show the reaction diagram
-
0.2% relative activity compared to the reaction with donor tamarind xyloglucan and acceptor xyloglucan oligosaccharide
-
-
?
donor hydroxyethylcellulose + acceptor xyloglucan oligosaccharide
?
show the reaction diagram
-
44.2% relative activity compared to the reaction with donor tamarind xyloglucan and acceptor xyloglucan oligosaccharide
-
-
?
donor locust bean gum + acceptor xyloglucan oligosaccharide
?
show the reaction diagram
-
0.1% relative activity compared to the reaction with donor tamarind xyloglucan and acceptor xyloglucan oligosaccharide
-
-
?
donor sodium carboxymethyl cellulose + acceptor xyloglucan
?
show the reaction diagram
-
-
-
-
?
donor sulfuric acid-swollen cellulose + acceptor xyloglucan oligosaccharide
?
show the reaction diagram
-
5% relative activity compared to the reaction with donor tamarind xyloglucan and acceptor xyloglucan oligosaccharide
-
-
?
donor tamarind xyloglucan + acceptor cellooligosaccharide
?
show the reaction diagram
-
0.1% relative activity compared to the reaction with donor tamarind xyloglucan and acceptor xyloglucan oligosaccharide
-
-
?
donor tamarind xyloglucan + acceptor xyloglucan
?
show the reaction diagram
-
isozymes XTH14 and XTH26 show very high preference for xyloglucan as donor substrate, yet do not work exclusively on xyloglucan, there is a clear preference for the octasaccharide XXLGol, whereas XLLGol is the second best (tested) substrate for isozyme XTH14, it seems a rather poor substrate for isozyme XTH26, which prefers XXFGol
-
-
?
donor tamarind xyloglucan + acceptor xyloglucan oligosaccharide
?
show the reaction diagram
-
-
-
-
?
donor xyloglucan + acceptor beta-(1,4)-xylooligosaccharide
?
show the reaction diagram
-
the transglycosylating activity is 0.31% of that determined with xyloglucan oligosaccharide as acceptor
-
-
?
donor xyloglucan + acceptor beta-(1,6)-D-gluco-oligosaccharide
?
show the reaction diagram
-
the transglycosylating activity is 0.69% of that determined with xyloglucan oligosaccharide as acceptor
-
-
?
donor xyloglucan + acceptor cellooligosaccharide
?
show the reaction diagram
-
-
-
-
?
donor xyloglucan + acceptor cellooligosaccharide
?
show the reaction diagram
-
the transglycosylating activity is 4.6% of that determined with xyloglucan oligosaccharide as acceptor
-
-
?
donor xyloglucan + acceptor laminarioligosaccharide
?
show the reaction diagram
-
-
-
-
?
donor xyloglucan + acceptor laminarioligosaccharide
?
show the reaction diagram
-
the transglycosylating activity is 0.23% of that determined with xyloglucan oligosaccharide as acceptor
-
-
?
donor xyloglucan + acceptor mixed-linkage beta-(1,3 1,4)-gluco-oligosaccharide
?
show the reaction diagram
-
the transglycosylating activity is 2.06% of that determined with xyloglucan oligosaccharide as acceptor
-
-
?
donor xyloglucan + acceptor pustulooligosaccharide
?
show the reaction diagram
-
-
-
-
?
donor xyloglucan + acceptor xyloglucan
?
show the reaction diagram
-
-
-
-
?
donor xyloglucan + acceptor xyloglucan
?
show the reaction diagram
-
-
-
-
?
donor xyloglucan + acceptor xyloglucan
?
show the reaction diagram
-
-
-
-
?
donor xyloglucan + acceptor xyloglucan
?
show the reaction diagram
-
-
-
-
?
donor xyloglucan + acceptor xyloglucan
?
show the reaction diagram
-
-
-
-
?
donor xyloglucan + acceptor xyloglucan
?
show the reaction diagram
-
-
-
-
?
donor xyloglucan + acceptor xyloglucan
?
show the reaction diagram
-
-
-
-
?
donor xyloglucan + acceptor xyloglucan
?
show the reaction diagram
-
-
-
-
?
donor xyloglucan + acceptor xyloglucan
?
show the reaction diagram
-
-
-
-
?
donor xyloglucan + acceptor xyloglucan
?
show the reaction diagram
-
-
-
-
?
donor xyloglucan + acceptor xyloglucan
?
show the reaction diagram
-
-
-
-
?
donor xyloglucan + acceptor xyloglucan
?
show the reaction diagram
-
-
-
-
?
donor xyloglucan + acceptor xyloglucan
?
show the reaction diagram
-
-
-
-
?
donor xyloglucan + acceptor xyloglucan
?
show the reaction diagram
-
-
-
-
?
donor xyloglucan + acceptor xyloglucan
?
show the reaction diagram
Q40144, -
-
-
-
?
donor xyloglucan + acceptor xyloglucan
?
show the reaction diagram
-
-
-
-
?
donor xyloglucan + acceptor xyloglucan
?
show the reaction diagram
-
-
-
-
?
donor xyloglucan + acceptor xyloglucan
?
show the reaction diagram
-
-
-
-
?
donor xyloglucan + acceptor xyloglucan
?
show the reaction diagram
-
-
-
-
?
donor xyloglucan + acceptor xyloglucan
?
show the reaction diagram
Q7Y252
-
-
-
?
donor xyloglucan + acceptor xyloglucan
?
show the reaction diagram
Q38909
-
-
-
?
donor xyloglucan + acceptor xyloglucan
?
show the reaction diagram
-
-
-
-
?
donor xyloglucan + acceptor xyloglucan
?
show the reaction diagram
-
acceptor specificity
-
-
?
donor xyloglucan + acceptor xyloglucan
?
show the reaction diagram
-
acceptor specificity
-
-
?
donor xyloglucan + acceptor xyloglucan
?
show the reaction diagram
-
acceptor specificity
-
-
?
donor xyloglucan + acceptor xyloglucan
?
show the reaction diagram
-
acceptor specificity
-
-
?
donor xyloglucan + acceptor xyloglucan
?
show the reaction diagram
-
kiwifruit xyloglucan donor, acceptor: XXXG-ol, a reduced heptasaccharide derived from kiwifruit xyloglucan
-
-
?
donor xyloglucan + acceptor xyloglucan
?
show the reaction diagram
-
acceptors: polymer xyloglucan or its derived oligosaccharides
-
-
?
donor xyloglucan + acceptor xyloglucan
?
show the reaction diagram
-
acceptors: polymer xyloglucan or its derived oligosaccharides
-
-
?
donor xyloglucan + acceptor xyloglucan
?
show the reaction diagram
-
acceptors: polymer xyloglucan or its derived oligosaccharides
-
-
?
donor xyloglucan + acceptor xyloglucan
?
show the reaction diagram
-
acceptors: polymer xyloglucan or its derived oligosaccharides
-
-
?
donor xyloglucan + acceptor xyloglucan
?
show the reaction diagram
-
acceptors: polymer xyloglucan or its derived oligosaccharides
-
-
?
donor xyloglucan + acceptor xyloglucan
?
show the reaction diagram
-
acceptors: polymer xyloglucan or its derived oligosaccharides
-
-
?
donor xyloglucan + acceptor xyloglucan
?
show the reaction diagram
-
acceptors: polymer xyloglucan or its derived oligosaccharides
-
-
?
donor xyloglucan + acceptor xyloglucan
?
show the reaction diagram
-
acceptors: polymer xyloglucan or its derived oligosaccharides
-
-
?
donor xyloglucan + acceptor xyloglucan
?
show the reaction diagram
-
acceptors: polymer xyloglucan or its derived oligosaccharides
-
-
?
donor xyloglucan + acceptor xyloglucan
?
show the reaction diagram
-
acceptors: polymer xyloglucan or its derived oligosaccharides
-
-
?
donor xyloglucan + acceptor xyloglucan
?
show the reaction diagram
-
acceptors: polymer xyloglucan or its derived oligosaccharides
-
-
?
donor xyloglucan + acceptor xyloglucan
?
show the reaction diagram
Sinapis sp.
-
acceptors: polymer xyloglucan or its derived oligosaccharides
-
-
?
donor xyloglucan + acceptor xyloglucan
?
show the reaction diagram
-
acceptors: polymer xyloglucan or its derived oligosaccharides
-
-
?
donor xyloglucan + acceptor xyloglucan
?
show the reaction diagram
Apium sp.
-
acceptors: polymer xyloglucan or its derived oligosaccharides
-
-
?
donor xyloglucan + acceptor xyloglucan
?
show the reaction diagram
-, Q9ZSU4
acceptor and donor substrate specificities of the isoenzymes TCH4, Meri-5, EXGT and XTR9
-
-
?
donor xyloglucan + acceptor xyloglucan
?
show the reaction diagram
P93349
NtXET-1 has a prefence for smaller xyloglucan molecules as acceptors
-
-
?
donor xyloglucan + acceptor xyloglucan
?
show the reaction diagram
-
acceptors: XXXG-ol, a reduced xyloglucan heptasaccharide, its dimer XXXGXXXG-ol, and other xyloglucan oligosaccharides, reaction probably involves an enzyme-donor-acceptor complex, in which the enzyme has two binding sites separately for donor and acceptor, mechanism
-
-
?
donor xyloglucan + acceptor xyloglucan
?
show the reaction diagram
-
enzyme requires a basic xyloglucan structure, i.e. a beta-1,4-glucosyl backbone with xylosyl side chains, for both acceptor and donor activity, higher activity when xyloglucans with higher MW are used as donor substrates, xyloglucans smaller than 10 kDa are no donor substrates, pyridylamino heptasaccharide, xyloglucan oligomers or polymers are good acceptors
-
-
?
donor xyloglucan + acceptor xyloglucan
?
show the reaction diagram
-
highly specific for xyloglucan as the glycosyl donor
-
-
?
donor xyloglucan + acceptor xyloglucan
?
show the reaction diagram
-
highly specific for xyloglucan as the glycosyl donor
-
-
?
donor xyloglucan + acceptor xyloglucan
?
show the reaction diagram
-
highly specific for xyloglucan as the glycosyl donor
-
-
?
donor xyloglucan + acceptor xyloglucan
?
show the reaction diagram
-
acceptor: fucosylated xyloglucan nonasaccharide
-
-
?
donor xyloglucan + acceptor xyloglucan
?
show the reaction diagram
-
pronounced preference for xyloglucan oligosaccharides with backbones of 4 over 3 over 2 Glc residues as acceptors
-
-
?
donor xyloglucan + acceptor xyloglucan
?
show the reaction diagram
-
xyloglucan from Tamarindus indica
-
-
?
donor xyloglucan + acceptor xyloglucan
?
show the reaction diagram
-
xyloglucan from Tamarindus indica
-
-
?
donor xyloglucan + acceptor xyloglucan
?
show the reaction diagram
-
xyloglucan from Tamarindus indica
-
-
?
donor xyloglucan + acceptor xyloglucan
?
show the reaction diagram
-
xyloglucan from Tamarindus indica
-
-
?
donor xyloglucan + acceptor xyloglucan
?
show the reaction diagram
-
xyloglucan from Tamarindus indica
-
-
?
donor xyloglucan + acceptor xyloglucan
?
show the reaction diagram
-
xyloglucan from Tamarindus indica
-
-
?
donor xyloglucan + acceptor xyloglucan
?
show the reaction diagram
-
xyloglucan from Tamarindus indica
-
-
?
donor xyloglucan + acceptor xyloglucan
?
show the reaction diagram
-
xyloglucan from Tamarindus indica
-
-
?
donor xyloglucan + acceptor xyloglucan
?
show the reaction diagram
-
xyloglucan from Tamarindus indica, xyloglucan from Vigna angularis
-
-
?
donor xyloglucan + acceptor xyloglucan
?
show the reaction diagram
-
donors: TCH4 protein is more active against tamarind xyloglucan than nasturtium xyloglucan as donor, pea stem xyloglucan, acceptors: XLLG-ol, XXFG-ol
-
-
?
donor xyloglucan + acceptor xyloglucan
?
show the reaction diagram
-
XET is present in cell walls in form of a competent glycosyl-enzyme complex which decomposes by transglycosylation of its glycan moiety to added xyloglucan-oligosaccharide acceptors
-
-
?
donor xyloglucan + acceptor xyloglucan
?
show the reaction diagram
-
XET forms a stable donor xyloglucan-XET complex
-
-
?
donor xyloglucan + acceptor xyloglucan
?
show the reaction diagram
-
preference for solanaceous xyloglucan without Fuc over xyloglucan from other sources as donor
-
-
?
donor xyloglucan + acceptor xyloglucan
?
show the reaction diagram
-
minimum acceptor structure is Xyl2Glc3
-
-
?
donor xyloglucan + acceptor xyloglucan
?
show the reaction diagram
-
minimum acceptor structure is Xyl2Glc3
-
-
?
donor xyloglucan + acceptor xyloglucan
?
show the reaction diagram
-
2 isoenzymes: sXET from seeds acts on xyloglucan molecules stochastically along the length of their polyglucose main chain and prefers low-MW xyloglucan-derived oligosaccharides as acceptors, eXET from epicotyls attacks the substrate predominantly near the reducing end and shows no preference for a size of xyloglucan oligosaccharide acceptors
-
-
?
donor xyloglucan + acceptor xyloglucan
?
show the reaction diagram
-, Q9ZSU4
isoenzyme XTR9 has a clear preference for non-fucosylated xyloglucan polymers as donor, but not isoenzymes TCH4, Meri-5 and EXGT
-
-
?
donor xyloglucan + acceptor xyloglucan
?
show the reaction diagram
-
enzyme chooses its donor substrate independently of size and attacks it, once only, at a randomly selected cleavage site
-
-
?
donor xyloglucan + acceptor xyloglucan
?
show the reaction diagram
-
xyloglucan from Rosa cultures
-
-
?
donor xyloglucan + acceptor xyloglucan
?
show the reaction diagram
-
xyloglucan from Tropaeolum majus seed
-
-
?
donor xyloglucan + acceptor xyloglucan
?
show the reaction diagram
-
xyloglucan from Tropaeolum majus seed
-
-
?
donor xyloglucan + acceptor xyloglucan
?
show the reaction diagram
-
xyloglucan from Tropaeolum majus seed
-
-
?
donor xyloglucan + acceptor xyloglucan
?
show the reaction diagram
-
xyloglucan from Tropaeolum majus seed
-
-
?
donor xyloglucan + acceptor xyloglucan
?
show the reaction diagram
-
xyloglucan from Tropaeolum majus seed
-
-
?
donor xyloglucan + acceptor xyloglucan
?
show the reaction diagram
-
xyloglucan from Tropaeolum majus seed
-
-
?
donor xyloglucan + acceptor xyloglucan
?
show the reaction diagram
-
donor specificity
-
-
?
donor xyloglucan + acceptor xyloglucan
?
show the reaction diagram
-
donor specificity
-
-
?
donor xyloglucan + acceptor xyloglucan
?
show the reaction diagram
-
donor specificity
-
-
?
donor xyloglucan + acceptor xyloglucan
?
show the reaction diagram
-
donor xyloglucan cleavage sites of XTHs from stems and epicotyls
-
-
?
donor xyloglucan + acceptor xyloglucan
?
show the reaction diagram
-
donor: Glc8-based XXXGXXXG
-
-
?
donor xyloglucan + acceptor xyloglucan
?
show the reaction diagram
-
enzyme transfers a large segment of a xyloglucan molecule to another one generating chimeric polymers
-
-
?
donor xyloglucan + acceptor xyloglucan
?
show the reaction diagram
-
enzyme transfers a large segment of a xyloglucan molecule to another one generating chimeric polymers
-
-
?
donor xyloglucan + acceptor xyloglucan
?
show the reaction diagram
-
enzyme transfers a large segment of a xyloglucan molecule to another one generating chimeric polymers
-
-
?
donor xyloglucan + acceptor xyloglucan
?
show the reaction diagram
-
galactosyl or fucosyl side chains are not required for acceptor activity
-
-
?
donor xyloglucan + acceptor xyloglucan
?
show the reaction diagram
-
galactosyl or fucosyl side chains are not required for acceptor activity
-
-
?
donor xyloglucan + acceptor xyloglucan
?
show the reaction diagram
-
xyloglucan from pea
-
-
?
donor xyloglucan + acceptor xyloglucan
?
show the reaction diagram
-
tamarind seed xyloglucan as donor substrate, oligosaccharidyl-alditol as acceptor
-
-
?
donor xyloglucan + acceptor xyloglucan
?
show the reaction diagram
-
specific for xyloglucan
-
-
?
donor xyloglucan + acceptor xyloglucan
?
show the reaction diagram
-
specific for xyloglucan
-
-
?
donor xyloglucan + acceptor xyloglucan
?
show the reaction diagram
-
acceptors: xyloglucan-derived nonasaccharide Glc4Xyl3GalFuc and certain other xyloglucan oligosaccharides, non-reducing terminal Xyl-Glc group is essential
-
-
?
donor xyloglucan + acceptor xyloglucan
?
show the reaction diagram
-
TCH4 protein specifically transglycosylates only xyloglucan, cleavage of both fucosylated and nonfucosylated xyloglucans as donor substrates, but fucosyl content of the substrates lowers reaction rate, preferred acceptor substrate is the nonreducing terminus of high-MW xyloglucan, xyloglucan derived oligosaccharides are also utilized
-
-
?
donor xyloglucan + acceptor xyloglucan
?
show the reaction diagram
-
acceptors: high MW xyloglucan, xyloglucan-derived oligosaccharides with at least two alpha-D-xylose residues, reducing group is not required for acceptor activity
-
-
?
donor xyloglucan + acceptor xyloglucan
?
show the reaction diagram
P93349
donor: pine or tobacco xyloglucan
-
-
?
donor xyloglucan + acceptor xyloglucan
?
show the reaction diagram
-
investigation of substrate subsite recognition requirements, xylose substitution at Glc +2 and -3 of the NXET cleavage site at the backbone of the xyloglucan substrate is a requirement, Gal substitution of a Xyl at +1 prevents, and at -2 modifies, chain-cleavage
-
-
?
donor xyloglucan + acceptor xyloglucan
?
show the reaction diagram
-
2 distinct XETs with different substrate prefences, XET from epicotyl, XET1, uses nonfucosylated seed amyloid xyloglucan or fucosylated stem xyloglucan as substrate with equal facility, XET from cotyledon, NXG1, has a significantly higher activity against nonfucosylated xyloglucan
-
-
?
donor xyloglucan + acceptor xyloglucan
?
show the reaction diagram
-
enzyme cleaves and religates xyloglucan polymers
-
-
?
donor xyloglucan + acceptor xyloglucan
?
show the reaction diagram
Q8GZD5
acceptors: sulforhodamine-labeled xyloglucan-oligosaccharides
-
-
?
donor xyloglucan + acceptor xyloglucan
?
show the reaction diagram
Q40144, Q6RHX7, Q6RHX8, Q6RHX9, Q6RHY0, Q6RHY1, Q9FR51, Q9FZ05, Q9SDX0, Q9SLN9
donor is apple xyloglycan, acceptor is XXXGol
-
-
?
donor xyloglucan + acceptor xyloglucan
?
show the reaction diagram
Pinus parviflora Glauca, Trigonella caerulea Ser., Pharbitis purpurea Bojer, Beckmannia syzigachne Fernald, Nicotiana tabacum SR-1, Cedrus atlantica Carr., Crocus vernus Hill
-
acceptors: xyloglucan oligosaccharide-SR mixture
-
-
?
donor xyloglucan + acceptor xyloglucan-derived oligosaccharide
?
show the reaction diagram
-
-
-
-
?
donor xyloglucan + Xyl(1-6)Glc(1-4)(Gal-Xyl(1-6))Glc(1-4)(Gal-Xyl(1-6))Glc(1-4)Glc-ol-sulphorhodamine conjugate
?
show the reaction diagram
-
XLLGol-SR
-
-
-
donor xyloglucan + Xyl(1-6)Glc(1-4)(Gal-Xyl(1-6))Glc(1-4)(Gal-Xyl(1-6))Glc(1-4)Glc-ol-sulphorhodamine conjugate
?
show the reaction diagram
-
XLLGol-SR
-
-
?
donor xyloglucan + Xyl(1-6)Glc(1-4)(Gal-Xyl(1-6))Glc(1-4)(Gal-Xyl(1-6))Glc(1-4)Glc-ol-sulphorhodamine conjugate
?
show the reaction diagram
Brassica oleracea, Sinapis sp., Apium sp.
-
XLLGol-SR
-
-
-
donor xyloglucan + Xyl(1-6)Glc(1-4)(Xyl(1-6))Glc(1-4)(Fuc-Gal-Xyl(1-6))Glc(1-4)Glc
?
show the reaction diagram
-
-
-
-
?
donor xyloglucan + Xyl(1-6)Glc(1-4)(Xyl(1-6))Glc(1-4)(Fuc-Gal-Xyl(1-6))Glc(1-4)Glc
?
show the reaction diagram
-
preference of XLLG over XXXG over XXFG over XXG
-
-
?
donor xyloglucan + Xyl(1-6)Glc(1-4)(Xyl(1-6))Glc(1-4)(Fuc-Gal-Xyl(1-6))Glc(1-4)Glc
?
show the reaction diagram
-
acceptor: xyloglucan-derived nonasaccharide XG9, Glc4Xyl3GalFuc, highly specific for xyloglucan as the glycosyl donor, xyloglucan from Tropaeolum seed is somewhat better than from Rosa cultures, enzyme transfers part of a large xyloglucan molecule to the nonasaccharide forming a polymer with a beta-1,4-linkage to the product and the acceptor group of the nonasaccharide is O-4 of the Glc residue furthest from the reducing terminus
-
-
?
donor xyloglucan + Xyl(1-6)Glc(1-4)(Xyl(1-6))Glc(1-4)(Fuc-Gal-Xyl(1-6))Glc(1-4)Glc
?
show the reaction diagram
-
XXFG
-
-
?
donor xyloglucan + Xyl(1-6)Glc(1-4)(Xyl(1-6))Glc(1-4)(Fuc-Gal-Xyl(1-6))Glc(1-4)Glc-ol
?
show the reaction diagram
-
XXFG-ol
-
-
?
donor xyloglucan + Xyl(1-6)Glc(1-4)(Xyl(1-6))Glc(1-4)(Fuc-Gal-Xyl(1-6))Glc(1-4)Glc-ol
?
show the reaction diagram
Q9ZSU4
XXFG-ol
-
-
?
donor xyloglucan + Xyl(1-6)Glc(1-4)(Xyl(1-6))Glc(1-4)(Fuc-Gal-Xyl(1-6))Glc(1-4)Glc-ol
?
show the reaction diagram
-
TCH4 protein, 50% as effective as XLLG-ol
-
-
?
donor xyloglucan + Xyl(1-6)Glc(1-4)(Xyl(1-6))Glc(1-4)(Fuc-Gal-Xyl(1-6))Glc(1-4)Glc-ol
?
show the reaction diagram
-, Q9ZSU4
TCH4, Meri-5, EXGT and XTR9 show a marked preference for XLLG-ol over XXFG-ol or XXXG-ol as acceptor oligosaccharide, TCH4, Meri-5 and EXGT: 25-30% as effective as XLLG-ol, less effective than XXXG-ol, XTR9: more effective than XXXG-ol, donor: tamarind or pea xyloglucan polymer
-
-
?
donor xyloglucan + Xyl(1-6)Glc(1-4)(Xyl(1-6))Glc(1-4)Glc
?
show the reaction diagram
-
-
-
-
?
donor xyloglucan + Xyl(1-6)Glc(1-4)(Xyl(1-6))Glc(1-4)Glc
?
show the reaction diagram
-
-
-
-
?
donor xyloglucan + Xyl(1-6)Glc(1-4)(Xyl(1-6))Glc(1-4)Glc
?
show the reaction diagram
-
Glc3Xyl2
-
-
?
donor xyloglucan + Xyl(1-6)Glc(1-4)(Xyl(1-6))Glc(1-4)Glc
?
show the reaction diagram
-
XXG, preference of XLLG over XXXG over XXFG over XXG
-
-
?
donor xyloglucan + Xyl(1-6)Glc(1-4)(Xyl(1-6))Glc(1-4)Glc
?
show the reaction diagram
-
Xyl2Glc3 is less effective than heptasaccharide XG7: Xyl3Glc4, minimal structure required for acceptor activity
-
-
?
donor xyloglucan + Xyl(1-6)Glc(1-4)(Xyl(1-6))Glc(1-4)Glc-ol
?
show the reaction diagram
-
pentamer XXG-ol, all isoenzymes have low but significant activity, tamarind xyloglucan as donor
-
-
?
donor xyloglucan + Xyl(1-6)Glc(1-4)Glc
?
show the reaction diagram
-
trimer XG-ol, only isomer M55a shows a slight activity, tamarind xyloglucan as donor
-
-
?
donor xyloglucan + Xylalpha(1-6)Glcbeta(1-4)(Galbeta(1-2)-Xylalpha(1-6))Glcbeta(1-4)(Galbeta(1-2)Xylalpha(1-6))Glcbeta(1-4)Glc-ol
?
show the reaction diagram
-
1.7-3.6fold preference of XLLG-ol over XXXG-ol, mung bean isoenzymes have higher affinities for XLLG-ol than cauliflower isoenzymes
-
-
?
donor xyloglucan + Xylalpha(1-6)Glcbeta(1-4)(Galbeta(1-2)-Xylalpha(1-6))Glcbeta(1-4)(Galbeta(1-2)Xylalpha(1-6))Glcbeta(1-4)Glc-ol
?
show the reaction diagram
-
comparison of the size distributions of the different isoenzymes transglycosylation products
-
-
?
donor xyloglucan + Xylalpha(1-6)Glcbeta(1-4)(Galbeta(1-2)-Xylalpha(1-6))Glcbeta(1-4)(Galbeta(1-2)Xylalpha(1-6))Glcbeta(1-4)Glc-ol
?
show the reaction diagram
-
isoenzyme C45a dissociates from the reaction products after each polysaccharide-to-oligosaccharide transglycosylation event
-
-
?
donor xyloglucan + Xylalpha(1-6)Glcbeta(1-4)(Galbeta(1-2)-Xylalpha(1-6))Glcbeta(1-4)(Galbeta(1-2)Xylalpha(1-6))Glcbeta(1-4)Glc-ol
?
show the reaction diagram
-
donor: tamarind xyloglucan
-
-
?
donor xyloglucan + Xylalpha(1-6)Glcbeta(1-4)(Galbeta(1-2)-Xylalpha(1-6))Glcbeta(1-4)(Galbeta(1-2)Xylalpha(1-6))Glcbeta(1-4)Glc-ol
?
show the reaction diagram
-
donor: tamarind xyloglucan
-
-
?
donor xyloglucan + Xylalpha(1-6)Glcbeta(1-4)(Galbeta(1-2)-Xylalpha(1-6))Glcbeta(1-4)(Galbeta(1-2)Xylalpha(1-6))Glcbeta(1-4)Glc-ol
?
show the reaction diagram
-
donor: tamarind xyloglucan
-
-
?
donor xyloglucan + Xylalpha(1-6)Glcbeta(1-4)(Galbeta(1-2)-Xylalpha(1-6))Glcbeta(1-4)(Galbeta(1-2)Xylalpha(1-6))Glcbeta(1-4)Glc-ol
?
show the reaction diagram
-
donor: tamarind xyloglucan
-
-
?
donor xyloglucan + Xylalpha(1-6)Glcbeta(1-4)(Galbeta(1-2)-Xylalpha(1-6))Glcbeta(1-4)(Galbeta(1-2)Xylalpha(1-6))Glcbeta(1-4)Glc-ol
?
show the reaction diagram
-, Q9ZSU4
TCH4, Meri-5, EXGT and XTR9 show a marked preference for XLLG-ol over XXFG-ol or XXXG-ol as acceptor oligosaccharide
-
-
?
donor xyloglucan + Xylalpha(1-6)Glcbeta(1-4)(Galbeta(1-2)-Xylalpha(1-6))Glcbeta(1-4)(Galbeta(1-2)Xylalpha(1-6))Glcbeta(1-4)Glc-ol
?
show the reaction diagram
-, Q9ZSU4
donor: tamarind or pea xyloglucan polymer
-
-
?
donor xyloglucan + Xylalpha(1-6)Glcbeta(1-4)(Galbeta(1-2)-Xylalpha(1-6))Glcbeta(1-4)(Galbeta(1-2)Xylalpha(1-6))Glcbeta(1-4)Glc-ol
?
show the reaction diagram
-
Gal2Xyl3Glc3glucitol prepared from tamarind flour/seed xyloglucan
-
-
?
donor xyloglucan + Xylalpha(1-6)Glcbeta(1-4)(Galbeta(1-2)-Xylalpha(1-6))Glcbeta(1-4)(Galbeta(1-2)Xylalpha(1-6))Glcbeta(1-4)Glc-ol
?
show the reaction diagram
-
Gal2Xyl3Glc3glucitol prepared from tamarind flour/seed xyloglucan
-
-
?
donor xyloglucan + Xylalpha(1-6)Glcbeta(1-4)(Galbeta(1-2)-Xylalpha(1-6))Glcbeta(1-4)(Galbeta(1-2)Xylalpha(1-6))Glcbeta(1-4)Glc-ol
?
show the reaction diagram
-
Gal2Xyl3Glc3glucitol prepared from tamarind flour/seed xyloglucan
-
-
?
donor xyloglucan + Xylalpha(1-6)Glcbeta(1-4)(Galbeta(1-2)-Xylalpha(1-6))Glcbeta(1-4)(Galbeta(1-2)Xylalpha(1-6))Glcbeta(1-4)Glc-ol
?
show the reaction diagram
-
Gal2Xyl3Glc3glucitol prepared from tamarind flour/seed xyloglucan
-
-
?
donor xyloglucan + Xylalpha(1-6)Glcbeta(1-4)(Galbeta(1-2)-Xylalpha(1-6))Glcbeta(1-4)(Galbeta(1-2)Xylalpha(1-6))Glcbeta(1-4)Glc-ol
?
show the reaction diagram
-
TCH4 protein, better substrate than XXFG-ol, lower affinity than for polymer xyloglucan
-
-
?
donor xyloglucan + Xylalpha(1-6)Glcbeta(1-4)(Galbeta(1-2)-Xylalpha(1-6))Glcbeta(1-4)(Galbeta(1-2)Xylalpha(1-6))Glcbeta(1-4)Glc-ol
?
show the reaction diagram
-
reaction results in a hybrid product and a leaving group
-
-
?
donor xyloglucan + Xylalpha(1-6)Glcbeta(1-4)(Galbeta(1-2)-Xylalpha(1-6))Glcbeta(1-4)(Galbeta(1-2)Xylalpha(1-6))Glcbeta(1-4)Glc-ol
?
show the reaction diagram
-
XLLG-ol
-
-
?
donor xyloglucan + Xylalpha(1-6)Glcbeta(1-4)(Galbeta(1-2)-Xylalpha(1-6))Glcbeta(1-4)(Galbeta(1-2)Xylalpha(1-6))Glcbeta(1-4)Glc-ol
?
show the reaction diagram
-
XLLG-ol
-
-
?
donor xyloglucan + Xylalpha(1-6)Glcbeta(1-4)(Galbeta(1-2)-Xylalpha(1-6))Glcbeta(1-4)(Galbeta(1-2)Xylalpha(1-6))Glcbeta(1-4)Glc-ol
?
show the reaction diagram
-
XLLG-ol
-
-
?
donor xyloglucan + Xylalpha(1-6)Glcbeta(1-4)(Galbeta(1-2)-Xylalpha(1-6))Glcbeta(1-4)(Galbeta(1-2)Xylalpha(1-6))Glcbeta(1-4)Glc-ol
?
show the reaction diagram
Q9ZSU4
XLLG-ol
-
-
?
donor xyloglucan + Xylalpha(1-6)Glcbeta(1-4)(Galbeta(1-2)-Xylalpha(1-6))Glcbeta(1-4)(Galbeta(1-2)Xylalpha(1-6))Glcbeta(1-4)Glc-ol
?
show the reaction diagram
-
XLLG-ol
-
-
?
donor xyloglucan + Xylalpha(1-6)Glcbeta(1-4)(Galbeta(1-2)-Xylalpha(1-6))Glcbeta(1-4)(Galbeta(1-2)Xylalpha(1-6))Glcbeta(1-4)Glc-ol
?
show the reaction diagram
-
XLLG-ol
-
-
?
donor xyloglucan + Xylalpha(1-6)Glcbeta(1-4)(Galbeta(1-2)-Xylalpha(1-6))Glcbeta(1-4)(Galbeta(1-2)Xylalpha(1-6))Glcbeta(1-4)Glc-ol
?
show the reaction diagram
-
XLLG-ol
-
-
?
donor xyloglucan + Xylalpha(1-6)Glcbeta(1-4)(Galbeta(1-2)-Xylalpha(1-6))Glcbeta(1-4)(Galbeta(1-2)Xylalpha(1-6))Glcbeta(1-4)Glc-ol
?
show the reaction diagram
-
XLLG-ol
-
-
?
donor xyloglucan + Xylalpha(1-6)Glcbeta(1-4)(Galbeta(1-2)Xylalpha(1-6))Glcbeta(1-4)(Galbeta(1-2)Xylalpha(1-6))Glcbeta(1-4)Glc
?
show the reaction diagram
-
-
-
-
?
donor xyloglucan + Xylalpha(1-6)Glcbeta(1-4)(Galbeta(1-2)Xylalpha(1-6))Glcbeta(1-4)(Galbeta(1-2)Xylalpha(1-6))Glcbeta(1-4)Glc
?
show the reaction diagram
-
preference of XLLG over XXXG over XXFG over XXG
-
-
?
donor xyloglucan + Xylalpha(1-6)Glcbeta(1-4)(Galbeta(1-2)Xylalpha(1-6))Glcbeta(1-4)(Galbeta(1-2)Xylalpha(1-6))Glcbeta(1-4)Glc
?
show the reaction diagram
-
XG9n, better substrate than Glc4Xyl3GalFuc
-
-
?
donor xyloglucan + Xylalpha(1-6)Glcbeta(1-4)(Galbeta(1-2)Xylalpha(1-6))Glcbeta(1-4)(Galbeta(1-2)Xylalpha(1-6))Glcbeta(1-4)Glc
?
show the reaction diagram
-
XLLG
-
-
?
donor xyloglucan + Xylalpha(1-6)Glcbeta(1-4)(Galbeta(1-2)Xylalpha(1-6))Glcbeta(1-4)(Galbeta(1-2)Xylalpha(1-6))Glcbeta(1-4)Glc
?
show the reaction diagram
-
XLLG
-
-
?
donor xyloglucan + Xylalpha(1-6)Glcbeta(1-4)(Galbeta(1-2)Xylalpha(1-6))Glcbeta(1-4)(Galbeta(1-2)Xylalpha(1-6))Glcbeta(1-4)Glc
?
show the reaction diagram
-
XLLG
-
-
?
donor xyloglucan + Xylalpha(1-6)Glcbeta(1-4)(Galbeta(1-2)Xylalpha(1-6))Glcbeta(1-4)(Galbeta(1-2)Xylalpha(1-6))Glcbeta(1-4)Glc
?
show the reaction diagram
-
XLLG from Tropaeolum majus seed, donor xyloglucan from tamarind cotyledons or pea stems, XET1 uses nonfucosylated tamarind seed amyloid xyloglucan or fucosylated pea stem xyloglucan as substrate with equal facility, NXG1 has a significantly higher activity against nonfucosylated tamarind xyloglucan
-
-
?
donor xyloglucan + Xylalpha(1-6)Glcbeta(1-4)(Galbeta(1-2)Xylalpha(1-6))Glcbeta(1-4)(Galbeta(1-2)Xylalpha(1-6))Glcbeta(1-4)Glc
?
show the reaction diagram
-
Glc4Xyl3Gal2
-
-
?
donor xyloglucan + Xylalpha(1-6)Glcbeta(1-4)(Galbeta(1-2)Xylalpha(1-6))Glcbeta(1-4)(Xylalpha(1-6))Glcbeta(1-4)Glc
?
show the reaction diagram
-
XLXG
-
-
?
donor xyloglucan + Xylalpha(1-6)Glcbeta(1-4)(Xylalpha(1-6))Glcbeta(1-4)(Galbeta(1-2)Xylalpha(1-6))Glcbeta(1-4)Glc
?
show the reaction diagram
-
XXLG
-
-
?
donor xyloglucan + Xylalpha(1-6)Glcbeta(1-4)(Xylalpha(1-6))Glcbeta(1-4)(Xylalpha(1-6))Glcbeta(1-4)Glc
?
show the reaction diagram
-
preference of XLLG over XXXG over XXFG over XXG
-
-
?
donor xyloglucan + Xylalpha(1-6)Glcbeta(1-4)(Xylalpha(1-6))Glcbeta(1-4)(Xylalpha(1-6))Glcbeta(1-4)Glc
?
show the reaction diagram
-
heptasaccharide XG7, Xyl3Glc4, is more effective than Xyl2Glc3
-
-
?
donor xyloglucan + Xylalpha(1-6)Glcbeta(1-4)(Xylalpha(1-6))Glcbeta(1-4)(Xylalpha(1-6))Glcbeta(1-4)Glc
?
show the reaction diagram
-
XG7, better substrate than Glc4Xyl3GalFuc
-
-
?
donor xyloglucan + Xylalpha(1-6)Glcbeta(1-4)(Xylalpha(1-6))Glcbeta(1-4)(Xylalpha(1-6))Glcbeta(1-4)Glc
?
show the reaction diagram
-
XXXG
-
-
?
donor xyloglucan + Xylalpha(1-6)Glcbeta(1-4)(Xylalpha(1-6))Glcbeta(1-4)(Xylalpha(1-6))Glcbeta(1-4)Glc
?
show the reaction diagram
-
XXXG
-
-
?
donor xyloglucan + Xylalpha(1-6)Glcbeta(1-4)(Xylalpha(1-6))Glcbeta(1-4)(Xylalpha(1-6))Glcbeta(1-4)Glc-ol
?
show the reaction diagram
-
-
-
-
?
donor xyloglucan + Xylalpha(1-6)Glcbeta(1-4)(Xylalpha(1-6))Glcbeta(1-4)(Xylalpha(1-6))Glcbeta(1-4)Glc-ol
?
show the reaction diagram
-
pea xyloglucan as donor, specific for xyloglucan as donor
-
-
?
donor xyloglucan + Xylalpha(1-6)Glcbeta(1-4)(Xylalpha(1-6))Glcbeta(1-4)(Xylalpha(1-6))Glcbeta(1-4)Glc-ol
?
show the reaction diagram
-, Q9ZSU4
TCH4, Meri-5, EXGT and XTR9 show a marked preference for XLLG-ol over XXFG-ol or XXXG-ol as acceptor oligosaccharide, TCH4, Meri-5 and EXGT: more effective than XXFG-ol, XTR9: less effective than XXFG-ol, donor: tamarind or pea xyloglucan polymer
-
-
?
donor xyloglucan + Xylalpha(1-6)Glcbeta(1-4)(Xylalpha(1-6))Glcbeta(1-4)(Xylalpha(1-6))Glcbeta(1-4)Glc-ol
?
show the reaction diagram
P93349
pine or tobacco xyloglucan as donor
-
-
?
donor xyloglucan + Xylalpha(1-6)Glcbeta(1-4)(Xylalpha(1-6))Glcbeta(1-4)(Xylalpha(1-6))Glcbeta(1-4)Glc-ol
?
show the reaction diagram
-
isoenzymes have 9-19times greater activities than on XXG-ol, reduced activity with XLLG-ol
-
-
?
donor xyloglucan + Xylalpha(1-6)Glcbeta(1-4)(Xylalpha(1-6))Glcbeta(1-4)(Xylalpha(1-6))Glcbeta(1-4)Glc-ol
?
show the reaction diagram
-
tamarind seed xyloglucan as donor
-
-
?
donor xyloglucan + Xylalpha(1-6)Glcbeta(1-4)(Xylalpha(1-6))Glcbeta(1-4)(Xylalpha(1-6))Glcbeta(1-4)Glc-ol
?
show the reaction diagram
-
tamarind seed xyloglucan as donor
-
-
?
donor xyloglucan + Xylalpha(1-6)Glcbeta(1-4)(Xylalpha(1-6))Glcbeta(1-4)(Xylalpha(1-6))Glcbeta(1-4)Glc-ol
?
show the reaction diagram
-
tamarind seed xyloglucan as donor
-
-
?
donor xyloglucan + Xylalpha(1-6)Glcbeta(1-4)(Xylalpha(1-6))Glcbeta(1-4)(Xylalpha(1-6))Glcbeta(1-4)Glc-ol
?
show the reaction diagram
-
XXXG-ol
-
-
?
donor xyloglucan + Xylalpha(1-6)Glcbeta(1-4)(Xylalpha(1-6))Glcbeta(1-4)(Xylalpha(1-6))Glcbeta(1-4)Glc-ol
?
show the reaction diagram
-
XXXG-ol
-
-
?
donor xyloglucan + Xylalpha(1-6)Glcbeta(1-4)(Xylalpha(1-6))Glcbeta(1-4)(Xylalpha(1-6))Glcbeta(1-4)Glc-ol
?
show the reaction diagram
-, Q9ZSU4
XXXG-ol
-
-
?
donor xyloglucan + Xylalpha(1-6)Glcbeta(1-4)(Xylalpha(1-6))Glcbeta(1-4)(Xylalpha(1-6))Glcbeta(1-4)Glc-ol
?
show the reaction diagram
-
XXXG-ol
-
-
?
donor xyloglucan + Xylalpha(1-6)Glcbeta(1-4)(Xylalpha(1-6))Glcbeta(1-4)(Xylalpha(1-6))Glcbeta(1-4)Glc-ol
?
show the reaction diagram
-
XXXG-ol
-
-
?
donor xyloglucan + Xylalpha(1-6)Glcbeta(1-4)(Xylalpha(1-6))Glcbeta(1-4)(Xylalpha(1-6))Glcbeta(1-4)Glc-ol
?
show the reaction diagram
-
XXXG-ol
-
-
?
donor xyloglucan + Xylalpha(1-6)Glcbeta(1-4)(Xylalpha(1-6))Glcbeta(1-4)(Xylalpha(1-6))Glcbeta(1-4)Glc-ol
?
show the reaction diagram
P93349
XXXG-ol
-
-
?
donor xyloglucan + Xylalpha(1-6)Glcbeta(1-4)(Xylalpha(1-6))Glcbeta(1-4)(Xylalpha(1-6))Glcbeta(1-4)Glc-ol
?
show the reaction diagram
-
XG7-ol is approximately as effective as heptasaccharide XG7, efficient substrate, reducing group is not required for acceptor activity
-
-
?
donor xyloglucan + Xylalpha(1-6)Glcbeta(1-4)(Xylalpha(1-6))Glcbeta(1-4)(Xylalpha(1-6))Glcbeta(1-4)Glc-ol
?
show the reaction diagram
-
transfers a part of xyloglucan to the reduced xyloglucan heptasaccharide XXXG-ol, the acceptor acts by combining with the enzyme independently of the donor, the velocity of the reaction decreases gradually as the heptasaccharide unit is increased from two to four, the affinity for XXXG-ol is increased at a higher concentration of donor xyloglucan
-
-
?
donor xyloglucan + Xylalpha(1-6)Glcbeta(1-4)(Xylalpha(1-6))Glcbeta(1-4)(Xylalpha(1-6))Glcbeta(1-4)Glcbeta(1-4)(Xylalpha(1-6))Glcbeta(1-4)(Xylalpha(1-6))Glcbeta(1-4)(Xylalpha(1-6))Glcbeta(1-4)Glc-ol
?
show the reaction diagram
-
XXXGXXXG-ol, dimer of XXXG-ol, most effective acceptor, pea xyloglucan as donor
-
-
?
donor xyloglucan + Xylalpha(1-6)Glcbeta(1-4)(Xylalpha(1-6))Glcbeta(1-4)(Xylalpha(1-6))Glcbeta(1-4)Glcbeta1-PA
?
show the reaction diagram
-
pyridylamino XXXG
-
-
?
donor xyloglucan + Xylalpha(1-6)Glcbeta(1-4)(Xylalpha(1-6))Glcbeta(1-4)(Xylalpha(1-6))Glcbeta(1-4)Glcbeta1-PA
?
show the reaction diagram
-
pyridylamino heptasaccharide is a good substrate
-
-
?
donor xyloglucan + xyloglucan
?
show the reaction diagram
B1P1S7, P93671, P93672
-
-
-
?
donor xyloglucan + xyloglucan acceptor
?
show the reaction diagram
-
-
-
-
-
donor xyloglucan + xyloglucan acceptor
?
show the reaction diagram
-
-
-
-
-
donor xyloglucan + xyloglucan acceptor
?
show the reaction diagram
-
-
-
-
-
donor xyloglucan + xyloglucan acceptor
?
show the reaction diagram
-
-
-
-
-
donor xyloglucan + xyloglucan acceptor
?
show the reaction diagram
-
-
-
-
-
donor xyloglucan + xyloglucan acceptor
?
show the reaction diagram
-
Arabidopsis MERI-5 and TCH4 and cauliflower isoenzymes expression occur in dense cytoplasmic tissues predominantly involved in the assembly of new cell walls and thus in the integration of newly secreted xyloglucan into the cell wall
-
-
-
donor xyloglucan + xyloglucan acceptor
?
show the reaction diagram
-
the xyloglucan:xyloglucosyl transferase gene TCH4 is strongly upregulated by environmental stimuli, enzyme may function in modifying cell walls to allow growth, airspace formation, the development of vasculature, and reinforcement of regions under mechanical strain, TCH4 protein may contribute to the adaptive changes in morphogenesis that occurs in the organism following exposure to mechanical stimuli
-
-
-
donor xyloglucan + xyloglucan acceptor
?
show the reaction diagram
-
2 isoenzymes: sXET from seeds plays a role in degrading xyloglucan reserves in seeds during germination, eXET from epicotyls is engaged in cell wall rearrangement and integration of new xyloglucan molecules into the preexisting cell wall structure during growth
-
-
-
donor xyloglucan + xyloglucan acceptor
?
show the reaction diagram
Q8GZD5
multifunctional role in cell wall construction, role in restructuring primary cell walls at the time when secondary wall layers are deposited, probably creating and reinforcing the connections between the primary and secondary wall layers
-
-
-
donor xyloglucan + xyloglucan acceptor
?
show the reaction diagram
-, Q9ZSU4
XETs encoded by a gene family may influence plant growth and development, low pH would limit XET function in vivo
-
-
-
donor xyloglucan + xyloglucan acceptor
?
show the reaction diagram
-
role in elongation-growth and other processes involving xyloglucan metabolism
-
-
-
donor xyloglucan + xyloglucan acceptor
?
show the reaction diagram
-
enzyme is unlikely to play a role in acid-induced wall loosening but may function in cold acclimation or cold-tolerant growth, TCH4 expression is rapidly regulated by mechanical stimuli, temperature shifts, light and hormones, it may be involved in assembling nascent cell walls or reinforcing existing and expanding walls
-
-
-
donor xyloglucan + xyloglucan acceptor
?
show the reaction diagram
P93349
NtXET-1 is involved in the incorporation of small xyloglucan molecules into the cell wall by transglycosylation, role during differentation and growth of the vascular tissue, reduced NtXET-1 expression and increase in the MW of xyloglucans in older leaves might be associated with strengthening of cell walls by reduced turnover and hydrolysis of xyloglucan, control of NtXET-1 expression in leaves
-
-
-
donor xyloglucan + xyloglucan acceptor
?
show the reaction diagram
-
activity increases markedly at the exponential growth and decreases immediately at the stationary phase of cells in presence of 2,4-dichlorophenoxyacetic acid, the activity is developmentally regulated during growth but is not directly induced by plant hormones
-
-
-
donor xyloglucan + xyloglucan acceptor
?
show the reaction diagram
-
important component of cell wall metabolism, particularly expanding tissue and ripening fruits, study on the role in tomato fruit ripening and vegetative growth, tXET-B1 may incorporate new xyloglucan into the existing network
-
-
-
donor xyloglucan + xyloglucan acceptor
?
show the reaction diagram
-
regulation of XET activity: enzyme exists in plant cell walls in a transiently latent state as covalent glycosyl-enzyme complex and is active only when suitable glycosyl acceptors become available
-
-
-
donor xyloglucan + xyloglucan acceptor
?
show the reaction diagram
-
existence of different classes of XET with differing roles in vivo
-
-
-
donor xyloglucan + xyloglucan acceptor
?
show the reaction diagram
-
existence of different classes of XET with differing roles in vivo
-
-
-
donor xyloglucan + xyloglucan acceptor
?
show the reaction diagram
-
existence of different classes of XET with differing roles in vivo
-
-
-
donor xyloglucan + xyloglucan acceptor
?
show the reaction diagram
-
existence of different classes of XET with differing roles in vivo
-
-
-
donor xyloglucan + xyloglucan acceptor
?
show the reaction diagram
-
XET and xyloglucan may play a role in the cell wall changes that accompany fruit softening during ripening, substrates for XET action are located in the cell wall
-
-
-
donor xyloglucan + xyloglucan acceptor
?
show the reaction diagram
-
role in cell elongation
-
-
-
donor xyloglucan + xyloglucan acceptor
?
show the reaction diagram
-
enzyme is capable of splitting and reconnecting xyloglucan molecules in rapidly growing plant tissues, expression and presumed physiological roles of At-XTH22 and 24
-
-
-
donor xyloglucan + xyloglucan acceptor
?
show the reaction diagram
Q40144, -
enzyme mediates cell wall disassembly associated with expansive growth
-
-
-
donor xyloglucan + xyloglucan acceptor
?
show the reaction diagram
-
key enzyme responsible for forming and rearranging the cellulose/xyloglucan network of the cell wall, commitment to the construction of both cell plate and cell wall
-
-
-
donor xyloglucan + xyloglucan acceptor
?
show the reaction diagram
-
enzyme depolymerises xyloglucan
-
-
-
donor xyloglucan + xyloglucan acceptor
?
show the reaction diagram
-
role in wall assembly as well as loosening
-
-
-
donor xyloglucan + xyloglucan acceptor
?
show the reaction diagram
-
enzyme is involved in the post-germinative mobilization of xyloglucan storage reserves, involved in cell wall loosening, activity is primarily regulated at the level of gene expression
-
-
-
donor xyloglucan + xyloglucan acceptor
?
show the reaction diagram
-
enzyme is responsible for cutting and rejoining intermicrofibrillar xyloglucan chains and thus causes the wall-loosening required for plant cell expansion and plant growth, in vivo the usual acceptor is polymeric wall-bound xyloglucan
-
-
-
donor xyloglucan + xyloglucan acceptor
?
show the reaction diagram
-
reconnecting enzyme for xyloglucans, involved in the interweaving or reconstruction of cell wall matrix, which is responsible for chemical creepage that leads to morphological changes in the cell wall, responsible for cell wall loosening and integration of cell wall architecture
-
-
-
donor xyloglucan + xyloglucan acceptor
?
show the reaction diagram
-
Arabidopsis EXGT and mung bean isoenzymes expression in tissues involved in the loosening of existing cell wall material necessary for rapid cell expansion, e.g. vacuolation
-
-
-
donor xyloglucan + xyloglucan acceptor
?
show the reaction diagram
-
lentil and nasturtium, NXG1, seed enzymes are involved in the mobilization of cotyledonary xyloglucan reserves after germination
-
-
-
donor xyloglucan + xyloglucan acceptor
?
show the reaction diagram
-
involved in xyloglucan metabolism
-
-
-
donor xyloglucan + xyloglucan acceptor
?
show the reaction diagram
-
one of four enzymes, which act in concert to catalyze the mobilisation of storage xyloglucan from the cell wall
-
-
-
donor xyloglucan + xyloglucan acceptor
?
show the reaction diagram
-
XET1 may play a role in cell-wall xyloglucan metabolism, such as the incorporation of newly synthesized xyloglucan into the expanding primary cell wall or the modification of xyloglucan polymers forming cross-links between cellulose microfibrils, NXG1 in vivo predominantly exhibits xyloglucanase activity and mobilizes nonfucosylated xyloglucan seed storage reserves
-
-
-
donor xyloglucan + xyloglucan acceptor
?
show the reaction diagram
-
major role is the integration of new xyloglucan into the cell walls of the densely cytoplasmic florets
-
-
-
donor xyloglucan + xyloglucan acceptor
?
show the reaction diagram
-
associated with primary cell wall metabolism, rather than mobilisation of any seed storage xyloglucan, major role is the re-structuring of existing wall material in the rapidly vacuolating shoots
-
-
-
donor xyloglucan + xyloglucan acceptor
?
show the reaction diagram
-
one of the key enzymes involved in the breakdown of reserve xyloglucan in seeds of some dicotyledonous plants during germination
-
-
-
donor xyloglucan + xyloglucan acceptor
?
show the reaction diagram
Pinus parviflora Glauca, Trigonella caerulea Ser., Pharbitis purpurea Bojer, Beckmannia syzigachne Fernald, Nicotiana tabacum SR-1, Cedrus atlantica Carr., Crocus vernus Hill
-
acts in root development, necessary for root hair growth
-
-
-
donor xyloglucan + [Xylp(alpha1,6)]-Glcp(beta1,4)-[Galp(beta1,2)-Xylp(alpha1,6)]-Glcp(beta1,4)-[Galp(beta1,2)-Xylp(alpha1,6)]-Glcp(beta1,4)-Glcp
?
show the reaction diagram
B1P1S7, P93671, P93672
-
-
-
?
donor xyloglucan + [Xylp(alpha1,6)]-Glcp(beta1,4)-[Xylp(alpha1,6)]-Glcp(beta1,4)-[Galp(beta1,2)-Xylp(alpha1,6)]-Glcp(beta1,4)-Glcp/[Xylp(alpha1,6)]-Glcp(beta1,4)-[Galp(beta1,2)-Xylp(alpha1,6)]-Glcp(beta1,4)-[Xylp(alpha1,6)]-Glcp(beta1,4)-Glcp
?
show the reaction diagram
B1P1S7, P93671, P93672
-
-
-
?
donor xyloglucan + [Xylp(alpha1,6)]-Glcp(beta1,4)-[Xylp(alpha1,6)]-Glcp(beta1,4)-[Xylp(alpha1,6)]-Glcp(beta1,4)-Glcp
?
show the reaction diagram
B1P1S7, P93671, P93672
-
-
-
?
Gal(beta1-4)Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)Glc + [Xyl(alpha1-6)]Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)Glc-8-aminonaphthalene-1,3,6-trisulfonic acid
[Xyl(alpha1-6)]Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)Glc + Gal(beta1-4)Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)Glc-8-aminonaphthalene-1,3,6-trisulfonic acid
show the reaction diagram
-
-
-
-
?
Gal(beta1-4)Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)Glc(beta1-4)Glc(beta1-4)Glc + [Xyl(alpha1-6)]Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)Glc-8-aminonaphthalene-1,3,6-trisulfonic acid
[Xyl(alpha1-6)]Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)Glc(beta1-4)Glc(beta1-4)Glc + Gal(beta1-4)Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)Glc-8-aminonaphthalene-1,3,6-trisulfonic acid
show the reaction diagram
-
-
-
-
?
Gal(beta1-4)Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)Glc(beta1-4)Glc(beta1-4)Glc + [Xyl(alpha1-6)]Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)Glc-8-aminonaphthalene-1,3,6-trisulfonic acid
Glc(beta1-4)Glc + Gal(beta1-4)Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)Glc-8-aminonaphthalene-1,3,6-trisulfonic acid
show the reaction diagram
-
-
-
-
?
Gal(beta1-4)Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)Glc + [Xyl(alpha1-6)]Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)Glc-8-aminonaphthalene-1,3,6-trisulfonic acid
[Xyl(alpha1-6)]Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)Glc + Gal(beta1-4)Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)Glc-8-aminonaphthalene-1,3,6-trisulfonic acid
show the reaction diagram
-
-
-
-
?
Gal(beta1-4)Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)Glc + [Xyl(alpha1-6)]Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)Glc-8-aminonaphthalene-1,3,6-trisulfonic acid
[Xyl(alpha1-6)]Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)Glc + Gal(beta1-4)Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)Glc-8-aminonaphthalene-1,3,6-trisulfonic acid
show the reaction diagram
-
-
-
-
?
Gal(beta1-4)Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)Glc + [Xyl(alpha1-6)]Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)Glc-8-aminonaphthalene-1,3,6-trisulfonic acid
[Xyl(alpha1-6)]Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)Glc + Gal(beta1-4)Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)Glc8-aminonaphthalene-1,3,6-trisulfonic acid
show the reaction diagram
-
-
-
-
?
Gal(beta1-4)Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)Glc + [Xyl(alpha1-6)]Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)Glc-8-aminonaphthalene-1,3,6-trisulfonic acid
Gal(beta1-4)Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)Glc + [Xyl(alpha1-6)]Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)Glc-8-aminonaphthalene-1,3,6-trisulfonic acid
show the reaction diagram
-
-
-
-
?
Glc(beta1-4)Glc(beta1-4)Glc(beta1-4)Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)Glc + [Xyl(alpha1-6)]Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)Glc-8-aminonaphthalene-1,3,6-trisulfonic acid
[Xyl(alpha1-6)]Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)Glc + Glc(beta1-4)Glc(beta1-4)Glc(beta1-4)Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)Glc-8-aminonaphthalene-1,3,6-trisulfonic acid
show the reaction diagram
-
-
-
-
?
Glc(beta1-4)Glc(beta1-4)Glc(beta1-4)Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)Glc + [Xyl(alpha1-6)]Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)Glc-8-aminonaphthalene-1,3,6-trisulfonic acid
Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)Glc + Glc(beta1-4)Glc(beta1-4)Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)Glc-8-aminonaphthalene-1,3,6-trisulfonic acid
show the reaction diagram
-
-
-
-
?
Glc(beta1-4)Glc(beta1-4)Glc(beta1-4)Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)Glc + [Xyl(alpha1-6)]Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)Glc-8-aminonaphthalene-1,3,6-trisulfonic acid
Glc(beta1-4)Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)Glc + Glc(beta1-4)Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)Glc-8-aminonaphthalene-1,3,6-trisulfonic acid
show the reaction diagram
-
-
-
-
?
Glc(beta1-4)Glc(beta1-4)Glc(beta1-4)Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)Glc + [Xyl(alpha1-6)]Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)Glc-8-aminonaphthalene-1,3,6-trisulfonic acid
Glc(beta1-4)Glc(beta1-4)Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)Glc + Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)Glc-8-aminonaphthalene-1,3,6-trisulfonic acid
show the reaction diagram
-
-
-
-
?
Glc(beta1-4)Glc(beta1-4)Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)Glc + [Xyl(alpha1-6)]Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)Glc-8-aminonaphthalene-1,3,6-trisulfonic acid
[Xyl(alpha1-6)]Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)Glc + Glc(beta1-4)Glc(beta1-4)Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)Glc-8-aminonaphthalene-1,3,6-trisulfonic acid
show the reaction diagram
-
-
-
-
?
Glc(beta1-4)Glc(beta1-4)Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)Glc + [Xyl(alpha1-6)]Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)Glc-8-aminonaphthalene-1,3,6-trisulfonic acid
Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)Glc + Glc(beta1-4)Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)Glc-8-aminonaphthalene-1,3,6-trisulfonic acid
show the reaction diagram
-
-
-
-
?
Glc(beta1-4)Glc(beta1-4)Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)Glc + [Xyl(alpha1-6)]Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)Glc-8-aminonaphthalene-1,3,6-trisulfonic acid
Glc(beta1-4)Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)Glc + Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)Glc-8-aminonaphthalene-1,3,6-trisulfonic acid
show the reaction diagram
-
-
-
-
?
Glc(beta1-4)Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)Glc + [Xyl(alpha1-6)]Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)Glc-8-aminonaphthalene-1,3,6-trisulfonic acid
[Xyl(alpha1-6)]Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)Glc + Glc(beta1-4)Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)Glc-8-aminonaphthalene-1,3,6-trisulfonic acid
show the reaction diagram
-
-
-
-
?
Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)Glc + [Xyl(alpha1-6)]Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)Glc-8-aminonaphthalene-1,3,6-trisulfonic acid
[Xyl(alpha1-6)]Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)Glc + Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)Glc-8-aminonaphthalene-1,3,6-trisulfonic acid
show the reaction diagram
-
-
-
-
?
Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)Glc + [Xyl(alpha1-6)]Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)Glc-8-aminonaphthalene-1,3,6-trisulfonic acid
[Xyl(alpha1-6)]Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)Glc + Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)Glc-8-aminonaphthalene-1,3,6-trisulfonic acid
show the reaction diagram
-
-
-
-
?
Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)Glc + [Xyl(alpha1-6)]Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)Glc-8-aminonaphthalene-1,3,6-trisulfonic acid
Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)Glc + [Xyl(alpha1-6)]Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)Glc-8-aminonaphthalene-1,3,6-trisulfonic acid
show the reaction diagram
-
-
-
-
?
Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)Glc + [Xyl(alpha1-6)]Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)Glc-8-aminonaphthalene-1,3,6-trisulfonic acid
[Xyl(alpha1-6)]Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)Glc + Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)Glc-8-aminonaphthalene-1,3,6-trisulfonic acid
show the reaction diagram
-
-
-
-
?
Glc8-based xyloglucan oligosaccharide + ?
?
show the reaction diagram
B1P1S7, P93668
-
-
-
-
hydroxyethyl cellulose + reduced xyloglucan-derived heptasaccharide
?
show the reaction diagram
B1P1S7, P93668
3.4% activity compared to tamarind xyloglycan
-
-
?
kiwifruit xyloglucan + XXXG-ol
?
show the reaction diagram
C0IRH4, C0IRH6, C0IRH7, C0IRH8, C0IRH9, C0IRI0, C0IRI1, C0IRI2, C0IRI3, C0IRI4, Q8GTJ0
-
-
-
?
kiwifruit xyloglucan + XXXG-ol
?
show the reaction diagram
C0IRG0, C0IRG2, C0IRH1
-
-
-
?
kiwifruit xyloglucan + XXXG-ol
?
show the reaction diagram
C0IRG1
-
-
-
?
kiwifruit xyloglucan + XXXG-ol
?
show the reaction diagram
C0IRG3, C0IRG4, C0IRG5, C0IRG6, C0IRG7, C0IRG9, C0IRH2
-
-
-
?
kiwifruit xyloglucan + XXXG-ol
?
show the reaction diagram
C0IRG8
-
-
-
?
kiwifruit xyloglucan + XXXG-ol
?
show the reaction diagram
C0IRH0, C0IRH3
-
-
-
?
pea xyloglucan + reduced xyloglucan-derived heptasaccharide
?
show the reaction diagram
B1P1S7, P93668
-
-
-
?
rhodamin-conjugated Glc3-Xyl3-Gal2-glucitol + xyloglucan
?
show the reaction diagram
-
acceptor substrate: rhodamin-conjugated reduced xyloglucan-derived nonasaccharide, donor substrate: tamarind-seed xyloglucan, fluorescence assay
-
-
?
tamarind xyloglucan + reduced xyloglucan-derived heptasaccharide
?
show the reaction diagram
B1P1S7, P93668
100% activity
-
-
?
tamarind xyloglucan + XLLG-9-aminopyrene-1,4,6-trisulfonate
?
show the reaction diagram
Q9ZV40
XET activity assay
-
-
?
tamarind xyloglucan + XXXG-ol
?
show the reaction diagram
C0IRH4, C0IRH6, C0IRH7, C0IRH8, C0IRH9, C0IRI0, C0IRI1, C0IRI2, C0IRI3, C0IRI4, Q8GTJ0
-
-
-
?
tamarind xyloglucan + XXXG-ol
?
show the reaction diagram
C0IRG0, C0IRG2, C0IRH1
-
-
-
?
tamarind xyloglucan + XXXG-ol
?
show the reaction diagram
C0IRG1
-
-
-
?
tamarind xyloglucan + XXXG-ol
?
show the reaction diagram
C0IRG3, C0IRG4, C0IRG5, C0IRG6, C0IRG7, C0IRG9, C0IRH2
-
-
-
?
tamarind xyloglucan + XXXG-ol
?
show the reaction diagram
C0IRG8
-
-
-
?
tamarind xyloglucan + XXXG-ol
?
show the reaction diagram
C0IRH0, C0IRH3
-
-
-
?
Xylalpha(1,6)Glcbeta(1,4)Xylalpha(1,6)Glcbeta(1,4)Xylalpha(1,6)Glcbeta(1,4)Glcbeta(1,4)Xylalpha(1,6)Glcbeta(1,4)Xylalpha(1,6)Glcbeta(1,4)Xylalpha(1,6)Glcbeta(1,4)Glcbeta(1,4) + Xylalpha(1,6)Glcbeta(1,4)Xylalpha(1,6)Glcbeta(1,4)Xylalpha(1,6)Glcbeta(1,4)Glcbeta(1,4) 8-aminonaphthalene-1,3,6-trisulfonic acid
Xylalpha(1,6)Glcbeta(1,4)Xylalpha(1,6)Glcbeta(1,4)Xylalpha(1,6)Glcbeta(1,4)Glcbeta(1,4)Xylalpha(1,6)Glcbeta(1,4)Xylalpha(1,6)Glcbeta(1,4)Xylalpha(1,6)Glcbeta(1,4)Glcbeta(1,4) 8-aminonaphthalene-1,3,6-trisulfonic acid + Xylalpha(1,6)Glcbeta(1,4)Xylalpha(1,6)Glcbeta(1,4)Xylalpha(1,6)Glcbeta(1,4)Glcbeta(1,4)
show the reaction diagram
-
-
-
-
?
Xylalpha(1-6)Glcbeta(1-4)(Xylalpha(1-6))Glcbeta(1-4)(Xylalpha(1-6))Glcbeta(1-4)Glcbeta(1-4)(Xylalpha(1-6))Glcbeta(1-4)(Xylalpha(1-6))Glcbeta(1-4)(Xylalpha(1-6))Glcbeta(1-4)Glc + Xylalpha(1-6)Glcbeta(1-4)(Xylalpha(1-6))Glcbeta(1-4)(Xylalpha(1-6))Glcbeta(1-4)Glcbeta(1-4)(Xylalpha(1-6))Glcbeta(1-4)(Xylalpha(1-6))Glcbeta(1-4)(Xylalpha(1-6))Glcbeta(1-4)Glc
Xylalpha(1-6)Glcbeta(1-4)(Xylalpha(1-6))Glcbeta(1-4)(Xylalpha(1-6))Glcbeta(1-4)Glcbeta(1-4)(Xylalpha(1-6))Glcbeta(1-4)(Xylalpha(1-6))Glcbeta(1-4)(Xylalpha(1-6))Glcbeta(1-4)Glcbeta(1-4)(Xylalpha(1-6))Glcbeta(1-4)(Xylalpha(1-6))Glcbeta(1-4)(Xylalpha(1-6))Glcbeta(1-4)Glc + Xylalpha(1-6)Glcbeta(1-4)(Xylalpha(1-6))Glcbeta(1-4)(Xylalpha(1-6))Glcbeta(1-4)Glc
show the reaction diagram
-
acceptor and donor: Glc8-based XXXGXXXG
products: XXXGXXXGXXXG + XXXG
?
xyloglucan + 4-mercaptobutanoyl-Xylalpha(1,6)Glc-Galalpha(1,2)Xylalpha(1,6)Glc-Galalpha(1,2)Xylalpha(1,6)Glc-Glc
?
show the reaction diagram
-
4-mercaptobutanoyl-Xylalpha(1,6)Glc-Galalpha(1,2)Xylalpha(1,6)Glc-Galalpha(1,2)Xylalpha(1,6)Glc-Glc is a worse acceptor than Xylalpha(1,6)Glc-Galalpha(1,2)Xylalpha(1,6)Glc-Galalpha(1,2)Xylalpha(1,6)Glc-Glc
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?
xyloglucan + barley (1,3 1,4)-beta-D-glucan
?
show the reaction diagram
-
the rate of covalent bond formation with barley (1,3 1,4)-beta-D-glucan is significant but slower compared with that on tamarind xyloglucan
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?
xyloglucan + heptaitol
?
show the reaction diagram
-
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?
xyloglucan + hydroxyethylcellulose
?
show the reaction diagram
-
the rate of covalent bond formation with hyrdoxyethylcellulose is comparable with that on tamarind xyloglucan
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-
?
xyloglucan + monostearyl-Xylalpha(1,6)Glc-Galalpha(1,2)Xylalpha(1,6)Glc-Galalpha(1,2)Xylalpha(1,6)Glc-Glc
?
show the reaction diagram
-
monostearyl-Xylalpha(1,6)Glc-Galalpha(1,2)Xylalpha(1,6)Glc-Galalpha(1,2)Xylalpha(1,6)Glc-Glc is a worse acceptor than Xylalpha(1,6)Glc-Galalpha(1,2)Xylalpha(1,6)Glc-Galalpha(1,2)Xylalpha(1,6)Glc-Glc
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?
xyloglucan + sulfuric acid-swollen cellulose
?
show the reaction diagram
-
-
-
-
?
xyloglucan + tamarind xyloglucan
?
show the reaction diagram
-
-
-
-
?
xyloglucan + Xylalpha(1,6)Glc-Galalpha(1,2)Xylalpha(1,6)Glc-Galalpha(1,2)Xylalpha(1,6)Glc-Glc
?
show the reaction diagram
-
-
-
-
?
xyloglucan + Xylalpha(1,6)Glc-Galalpha(1,2)Xylalpha(1,6)Glc-Galalpha(1,2)Xylalpha(1,6)Glc-Glc
?
show the reaction diagram
-
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?
xyloglucan + Xylalpha(1,6)Glc-Xylalpha(1,6)Glc-Galalpha(1,2)Xylalpha(1,6)Glc-Glc
?
show the reaction diagram
-
-
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-
?
xyloglucan nonasaccharide + xyloglucan
?
show the reaction diagram
Q8GZD5
acceptor: nonasaccharide XLLGol, donor: tamarind xyloglucan
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?
xyloglucan nonasaccharide + xyloglucan
?
show the reaction diagram
-
nonasaccharide XLLG, a 2-chloro-4-nitrophenyl-nonasaccharide is no substrate, tamarind xyloglucan
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?
xyloglucan oligosaccharides + xyloglucan
?
show the reaction diagram
-
-
-
-
?
xyloglucan oligosaccharides + xyloglucan
?
show the reaction diagram
-, Q5Z6G7, Q5Z6H1, Q5Z6H3, Q6ZAN9, Q763W5, Q76BW5, Q8VWG5
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-
?
xyloglucan oligosaccharides + xyloglucan
?
show the reaction diagram
-
-
-
-
?
xyloglucan oligosaccharides + xyloglucan
?
show the reaction diagram
-, Q6YDN9
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-
?
xyloglucan oligosaccharides + xyloglucan
?
show the reaction diagram
-
sulforhodamine-labeled xyloglucan-derived oligosaccharides, and tamarind-seed xyloglucan
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?
xyloglucan oligosaccharides + xyloglucan
? + xyloglucan
show the reaction diagram
-
xyloglucans with MW of about 600-1000 kDa
MW about 50 kDa
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?
[Xyl(alpha1-6)]Glc(beta1-4)Glc(beta1-4)Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)Glc + [Xyl(alpha1-6)]Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)Glc-8-aminonaphthalene-1,3,6-trisulfonic acid
[Xyl(alpha1-6)]Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)Glc + [Xyl(alpha1-6)]Glc(beta1-4)Glc(beta1-4)Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)Glc-8-aminonaphthalene-1,3,6-trisulfonic acid
show the reaction diagram
-
-
-
-
?
[Xyl(alpha1-6)]Glc(beta1-4)Glc(beta1-4)Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)Glc + [Xyl(alpha1-6)]Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)Glc-8-aminonaphthalene-1,3,6-trisulfonic acid
[Xyl(alpha1-6)]Glc(beta1-4)Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)Glc + Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)Glc-8-aminonaphthalene-1,3,6-trisulfonic acid
show the reaction diagram
-
-
-
-
?
[Xyl(alpha1-6)]Glc(beta1-4)Glc(beta1-4)Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)Glc + [Xyl(alpha1-6)]Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)Glc-8-aminonaphthalene-1,3,6-trisulfonic acid
Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)Glc + [Xyl(alpha1-6)]Glc(beta1-4)Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)Glc-8-aminonaphthalene-1,3,6-trisulfonic acid
show the reaction diagram
-
-
-
-
?
[Xyl(alpha1-6)]Glc(beta1-4)Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)Glc + [Xyl(alpha1-6)]Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)Glc-8-aminonaphthalene-1,3,6-trisulfonic acid
[Xyl(alpha1-6)]Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)Glc + [Xyl(alpha1-6)]Glc(beta1-4)Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)Glc-8-aminonaphthalene-1,3,6-trisulfonic acid
show the reaction diagram
-
-
-
-
?
[Xyl(alpha1-6)]Glc(beta1-4)Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)Glc + [Xyl(alpha1-6)]Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)Glc-8-aminonaphthalene-1,3,6-trisulfonic acid
[Xyl(alpha1-6)]Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)Glc + [Xyl(alpha1-6)]Glc(beta1-4)Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)Glc-8-aminonaphthalene-1,3,6-trisulfonic acid
show the reaction diagram
-
-
-
-
?
[Xyl(alpha1-6)]Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)Glc + [Xyl(alpha1-6)]Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)Glc-8-aminonaphthalene-1,3,6-trisulfonic acid
[Xyl(alpha1-6)]Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)Glc + [Xyl(alpha1-6)]Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)Glc-8-aminonaphthalene-1,3,6-trisulfonic acid
show the reaction diagram
-
-
-
-
?
[Xyl(alpha1-6)]Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)Glc + [Xyl(alpha1-6)]Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)Glc-8-aminonaphthalene-1,3,6-trisulfonic acid
Xyl(alpha1-6)Glc + [Xyl(alpha1-6)]Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)Glc-8-aminonaphthalene-1,3,6-trisulfonic acid
show the reaction diagram
-
-
-
-
?
[Xyl(alpha1-6)]Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)Glc(beta1-4)Glc(beta1-4)Glc + [Xyl(alpha1-6)]Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)Glc-8-aminonaphthalene-1,3,6-trisulfonic acid
Glc(beta1-4)Glc + [Xyl(alpha1-6)]Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)Glc-8-aminonaphthalene-1,3,6-trisulfonic acid
show the reaction diagram
-
-
-
-
?
[Xyl(alpha1-6)]Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)Glc(beta1-4)Glc(beta1-4)Glc + [Xyl(alpha1-6)]Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)Glc-8-aminonaphthalene-1,3,6-trisulfonic acid
Glc(beta1-4)Glc + [Xyl(alpha1-6)]Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)Glc-8-aminonaphthalene-1,3,6-trisulfonic acid
show the reaction diagram
-
-
-
-
?
[Xyl(alpha1-6)]Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)Glc(beta1-4)Glc(beta1-4)Glc(beta1-4)Glc(beta1-4)Glc + [Xyl(alpha1-6)]Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)Glc-8-aminonaphthalene-1,3,6-trisulfonic acid
Glc(beta1-4)Glc(beta1-4)Glc(beta1-4)Glc + [Xyl(alpha1-6)]Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)Glc-8-aminonaphthalene-1,3,6-trisulfonic acid
show the reaction diagram
-
-
-
-
?
[Xyl(alpha1-6)]Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)Glc(beta1-4)[Xyl(alpha1-6)]Glc + [Xyl(alpha1-6)]Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)Glc-8-aminonaphthalene-1,3,6-trisulfonic acid
[Xyl(alpha1-6)]Glc(beta1-4)Glc + [Xyl(alpha1-6)]Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)Glc-8-aminonaphthalene-1,3,6-trisulfonic acid
show the reaction diagram
-
-
-
-
?
[Xyl(alpha1-6)]Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)Glc + [Xyl(alpha1-6)]Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)Glc-8-aminonaphthalene-1,3,6-trisulfonic acid
[Xyl(alpha1-6)]Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)Glc + [Xyl(alpha1-6)]Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)Glc-8-aminonaphthalene-1,3,6-trisulfonic acid
show the reaction diagram
-
-
-
-
?
[Xyl(alpha1-6)]Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)Glc + [Xyl(alpha1-6)]Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)Glc-8-aminonaphthalene-1,3,6-trisulfonic acid
[Xyl(alpha1-6)]Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)Glc + [Xyl(alpha1-6)]Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)Glc-8-aminonaphthalene-1,3,6-trisulfonic acid
show the reaction diagram
-
-
-
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?
[Xyl(alpha1-6)]Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)Glc + [Xyl(alpha1-6)]Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)Glc-8-aminonaphthalene-1,3,6-trisulfonic acid
[Xyl(alpha1-6)]Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)Glc + [Xyl(alpha1-6)]Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)Glc-8-aminonaphthalene-1,3,6-trisulfonic acid
show the reaction diagram
-
-
-
-
?
[Xyl(alpha1-6)]Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)Glc + [Xyl(alpha1-6)]Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)Glc-8-aminonaphthalene-1,3,6-trisulfonic acid
[Xyl(alpha1-6)]Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)Glc + [Xyl(alpha1-6)]Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)Glc-8-aminonaphthalene-1,3,6-trisulfonic acid
show the reaction diagram
-
-
-
-
?
[Xyl(alpha1-6)]Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)Glc + [Xyl(alpha1-6)]Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)Glc-8-aminonaphthalene-1,3,6-trisulfonic acid
[Xyl(alpha1-6)]Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)Glc-8-aminonaphthalene-1,3,6-trisulfonic acid
show the reaction diagram
-
-
-
-
?
[Xyl(alpha1-6)]Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)Glc + [Xyl(alpha1-6)]Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)Glc-8-aminonaphthalene-1,3,6-trisulfonic acid
[Xyl(alpha1-6)]Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)Glc + [Xyl(alpha1-6)]Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)Glc-8-aminonaphthalene-1,3,6-trisulfonic acid
show the reaction diagram
-
-
-
-
?
[Xyl(alpha1-6)]Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)Glc + [Xyl(alpha1-6)]Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)Glc-8-aminonaphthalene-1,3,6-trisulfonic acid
[Xyl(alpha1-6)]Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)Glc + [Xyl(alpha1-6)]Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)Glc-8-aminonaphthalene-1,3,6-trisulfonic acid
show the reaction diagram
-
-
-
-
?
[Xyl(alpha1-6)]Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)Glc + [Xyl(alpha1-6)]Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)Glc-8-aminonaphthalene-1,3,6-trisulfonic acid
[Xyl(alpha1-6)]Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)Glc-8-aminonaphthalene-1,3,6-trisulfonic acid
show the reaction diagram
-
-
-
-
?
additional information
?
-
-
-
-
-
-
additional information
?
-
-
not: Xyl1Glc3, XylGlc2
-
-
-
additional information
?
-
-
NXET has also a xyloglucan-specific hydrolase activity, cleavage sites for hydrolysis and endotransglycosylation are identical, hydrolase action is significantly only under conditions, where suitable acceptor chain-end concentrations are limiting
-
-
-
additional information
?
-
-
not: carboxymethylcellulose, barley glucan
-
-
-
additional information
?
-
-
tXET-B2 has no hydrolytic activity
-
-
-
additional information
?
-
-
no action on other poly- and oligosaccharides than xyloglucan, such as cellulose, its soluble derivatives, mixed linkage beta-glucans, cello-oligosaccharides, enzyme catalyzes, additional to the powerful and specific xyloglucan endo-transglycosylase action, the hydrolysis of tamarind xyloglucan to a mixture of oligosaccharides of different sizes, hydrolysis increases at low substrate concentrations
-
-
-
additional information
?
-
-, Q9ZSU4
contains strongly conserved sequence motif of XETs: DEIDFEFLG
-
-
-
additional information
?
-
Q8GZD5
contains strongly conserved sequence motif of XETs: DEIDFEFLG
-
-
-
additional information
?
-
-, Q9ZSU4
no endoglucanase activity
-
-
-
additional information
?
-
-
oligosaccharides of cello-, chito- and/or oligoglucurono-series are much less effective than xyloglucan-derived oligosaccharides
-
-
-
additional information
?
-
Q8GZD5
PttXET16A has 4 conserved Cys residues
-
-
-
additional information
?
-
-
in absence of reduced xyloglucan-derived heptasaccharide XXXG-ol enzyme depolymerizes xyloglucan by hydrolysis and in the presence of it by both hydrolysis and endotransglycosylation
-
-
-
additional information
?
-
-
no glycosidase or glycanase activity, no donors: xyloglucans smaller than 10 kDa, carboxymethylcellulose, Avena glucan, maize xylan, Rhodymenia xylan, no acceptors: pyridylamino cellohexaose, pyridylamino laminarihexaose
-
-
-
additional information
?
-
-
no acceptors: alpha-D-Xylp(1-6)D-Glc and isoprimeverose
-
-
-
additional information
?
-
-
not: cellotetraose
-
-
-
additional information
?
-
-
not: cellotetraose
-
-
-
additional information
?
-
-
free oligosaccharides are probably not the usual acceptor substrates in vivo
-
-
-
additional information
?
-
-
acidic polymers, probably acidic arabinogalactan proteins, and/or other apoplastic ions are naturally occurring regulators of the enzyme activity in vivo, and may thus control cell wall assembly, loosening, and growth
-
-
-
additional information
?
-
-
key enzyme in all plant processes that require cell wall remodeling
-
-
-
additional information
?
-
-
xyloglucan oligosaccharides cause cell wall loosening by enhancing xyloglucan oligosaccharide degrading enzyme activity
-
-
-
additional information
?
-
-, Q6YDN9
different assay methods, overview
-
-
-
additional information
?
-
-
isozymes sXET and eXET show different specificities for xyloglucan-derived oligosaccharide acceptor substrates, and distinct preferences in selecting the site of attack on xyloglucan molecules
-
-
-
additional information
?
-
Q8GZD5
no hydrolytic activity of the wild-type or N93S mutant enzyme
-
-
-
additional information
?
-
-
the enzyme shows both xyloglucan endotransglucosylase and xyloglucan hydrolase activity
-
-
-
additional information
?
-
-, Q5Z6G7, Q5Z6H1, Q5Z6H3, Q6ZAN9, Q763W5, Q76BW5, Q8VWG5
the enzyme shows both xyloglucan endotransglucosylase and xyloglucan hydrolase activity
-
-
-
additional information
?
-
-
the enzyme shows both xyloglucan endotransglucosylase and xyloglucan hydrolase activity
-
-
-
additional information
?
-
-
the enzyme shows both xyloglucan endotransglucosylase and xyloglucan hydrolase activity
-
-
-
additional information
?
-
Piper sp., Lactuca sativa ssp. capitata
-
the enzyme shows both xyloglucan endotransglucosylase and xyloglucan hydrolase activity
-
-
-
additional information
?
-
-
transglycosylation acceptor binding mechanism, structure-function study
-
-
-
additional information
?
-
-
does not produce any hetero-transglycosylation products with cello-oligosaccharides or lactose
-
-
-
additional information
?
-
-
no transferase activities are detected with laminarin, lichenin, pustulan, barley arabinoxylan, Konjac glucomannan, citrus fruit pectin, and esterified pectin (potassium salt), orange polygalactouronic acid (sodium salt), soybean pectic fiber rhamnogalactouronan, beta-D-galactans, and arabinogalactan protein with xyloglucan oligosaccharide and cellooligosaccharide, no transferase activity is detected laminari-oligosaccharides as acceptor substrates
-
-
-
additional information
?
-
-
breaks a beta-(1->4) bond in the backbone of a xyloglucan and transfers the xyloglucanyl segment on to O-4 of the non-reducing terminal glucose residue of an acceptor, which can be a xyloglucan or an oligosaccharide of xyloglucan
-
-
-
additional information
?
-
Q5JZX2, -
breaks a beta-(1->4) bond in the backbone of a xyloglucan and transfers the xyloglucanyl segment on to O-4 of the non-reducing terminal glucose residue of an acceptor, which can be a xyloglucan or an oligosaccharide of xyloglucan
-
-
-
additional information
?
-
-
breaks a beta-(1->4) bond in the backbone of a xyloglucan and transfers the xyloglucanyl segment on to O-4 of the non-reducing terminal glucose residue of an acceptor, which can be a xyloglucan or an oligosaccharide of xyloglucan
-
-
-
additional information
?
-
Q40144, Q43527, Q43528, Q6RHX7, Q6RHX8, Q6RHX9, Q6RHY0, Q6RHY1, Q9FR51, Q9FZ05, Q9SDX0, Q9SLN9
breaks a beta-(1->4) bond in the backbone of a xyloglucan and transfers the xyloglucanyl segment on to O-4 of the non-reducing terminal glucose residue of an acceptor, which can be a xyloglucan or an oligosaccharide of xyloglucan
-
-
-
additional information
?
-
-
breaks a beta-(1->4) bond in the backbone of a xyloglucan and transfers the xyloglucanyl segment on to O-4 of the non-reducing terminal glucose residue of an acceptor, which can be a xyloglucan or an oligosaccharide of xyloglucan
-
-
-
additional information
?
-
Q9ZV40
breaks a beta-(1->4) bond in the backbone of a xyloglucan and transfers the xyloglucanyl segment on to O-4 of the non-reducing terminal glucose residue of an acceptor, which can be a xyloglucan or an oligosaccharide of xyloglucan
-
-
-
additional information
?
-
-
breaks a beta-(1->4) bond in the backbone of a xyloglucan and transfers the xyloglucanyl segment on to O-4 of the non-reducing terminal glucose residue of an acceptor, which can be a xyloglucan or an oligosaccharide of xyloglucan
-
-
-
additional information
?
-
Q1XD17, -
breaks a beta-(1->4) bond in the backbone of a xyloglucan and transfers the xyloglucanyl segment on to O-4 of the non-reducing terminal glucose residue of an acceptor, which can be a xyloglucan or an oligosaccharide of xyloglucan
-
-
-
additional information
?
-
-
breaks a beta-(1-4) bond in the backbone of a xyloglucan and transfers the xyloglucanyl segment on to O-4 of the non-reducing terminal glucose residue of an acceptor, which can be a xyloglucan or an oligosaccharide of xyloglucan
-
-
-
additional information
?
-
P24806
anionic, oxidised derivatives of xyloglucan are used as substrates for AtXTH24
-
-
-
additional information
?
-
-
isozyme XTH14 uses donor substrates in order of preference xyloglucan >> watersoluble cellulose acetate > hydroxyethylcellulose > mixed-linkage beta-glucan > carboxymethylcellulose > methylcellulose, thus, all nonxyloglucan substrates show very low activity rates in comparison to xyloglucan. Isozyme XTH26 is able to use watersoluble cellulose acetate at a low rate but shows no activity with any of the other non-xyloglucan substrates tested
-
-
-
additional information
?
-
B1P1S7, P93668
no activity towards barley 1,3-1,4-beta-D-glucan (high viscosity), and locust bean gum galactomannan, no transferase activities are detected with laminarin, lichenin, pustulan, barley arabinoxylan, Konjac glucomannan, citrus fruit pectin and esterified pectin (potassium salt), orange polygalacturonic acid (sodium salt), soybean pectic fibre rhamnogalactouronan, beta-D-galactans (from lupin and potato) and arabinogalactan protein (from gum arabic)
-
-
-
additional information
?
-
B1P1S7, P93668
no hydrolytic activity can be detected with either isoenzyme
-
-
-
additional information
?
-
-
no transferase activity is detected with laminarin, lichenin, pustulan, barley arabinoxylan, glucomannan, citrus fruit pectin or esterified pectin (potassium salt), orange polygalacturonic acid (sodium salt), soybean pectic fiber rhamnogalactouronan, lupin or potato beta-D-galactans, or gum arabic
-
-
-
additional information
?
-
-
the enzyme exhibits broad substrate specificity by transferring xyloglucan or hydroxyethylcellulose fragments not only to oligoxyloglucosides and cellooligosaccharides but also to oligosaccharides derived from beta-(1,4)-D-glucuronoxylan, beta-(1,6)-D-glucan, mixed-linkage beta-(1,3 1,4)-D-glucan and at a relatively low rate also to beta-(1,3)-gluco-oligosaccharides
-
-
-
additional information
?
-
-
XET5 catalyzes the in vitro formation of covalent linkages between xyloglucans and cellulosic substrates and between xyloglucans and (1,3 1,4)-beta-D-glucans
-
-
-
additional information
?
-
O80803, Q9FKL8, Q9FKL9, Q9M0D1, Q9M0D2
root-specific isozyme AtXTH1 exhibits only the endotransglucosylase activity, XET, EC 2.4.1.207, towards xyloglucan and non-detectable endohydrolytic activity, XEH, EC 3.2.1.151. Its endotransglucosylase activity is preferentially directed towards xyloglucan and water-soluble cellulose acetate, rather than to mixed-linkage beta-glucan
-
-
-
additional information
?
-
O80803, Q9FKL8, Q9FKL9, Q9M0D1, Q9M0D2
root-specific isozyme AtXTH13 exhibits only the endotransglucosylase activity, XET, EC 2.4.1.207, towards xyloglucan and non-detectable endohydrolytic activity, XEH, EC 3.2.1.151. The endotransglucosylase activity is preferentially directed towards xyloglucan and water-soluble cellulose acetate, rather than to mixed-linkage beta-glucan
-
-
-
additional information
?
-
O80803, Q9FKL8, Q9FKL9, Q9M0D1, Q9M0D2
root-specific isozyme AtXTH17 exhibits only the endotransglucosylase activity, XET, EC 2.4.1.207, towards xyloglucan and non-detectable endohydrolytic activity, XEH, EC 3.2.1.151. Its endotransglucosylase activity is preferentially directed towards xyloglucan and water-soluble cellulose acetate, rather than to mixed-linkage beta-glucan
-
-
-
additional information
?
-
O80803, Q9FKL8, Q9FKL9, Q9M0D1, Q9M0D2
root-specific isozyme AtXTH18 exhibits only the endotransglucosylase activity, XET, EC 2.4.1.207, towards xyloglucan and non-detectable endohydrolytic activity, XEH, EC 3.2.1.151. Its endotransglucosylase activity is preferentially directed towards xyloglucan and water-soluble cellulose acetate, rather than to mixed-linkage beta-glucan
-
-
-
additional information
?
-
O80803, Q9FKL8, Q9FKL9, Q9M0D1, Q9M0D2
root-specific isozyme AtXTH19 exhibits only the endotransglucosylase activity, XET, EC 2.4.1.207, towards xyloglucan and non-detectable endohydrolytic activity, XEH, EC 3.2.1.151. Its endotransglucosylase activity is preferentially directed towards xyloglucan and water-soluble cellulose acetate, rather than to mixed-linkage beta-glucan
-
-
-
additional information
?
-
-
XET activity catalyses the endo-cleavage of xyloglucan (the donor substrate) and the creation of a glycosidic link between the newly formed potentially reducing terminus and the nonreducing terminus of another xyloglucan (or xyloglucan oligosaccharide) molecule (the acceptor substrate), cleavage specificity, overview
-
-
-
additional information
?
-
-
activity assay with reduced xyloglucan-derived nonasaccharide as substrate
-
-
-
additional information
?
-
P93349
usage of xyloglucan heptasaccharide XXXG and its reduced form XXXGol as donor substrates
-
-
-
additional information
?
-
O80803, Q9FKL8, Q9FKL9, Q9M0D1, Q9M0D2
XTH isozyme spcificities with different donor substrates, tamarind xyloglucan-derived oligosacchrides, overview
-
-
-
additional information
?
-
O80803, Q9FKL8, Q9FKL9, Q9M0D1, Q9M0D2
XTH isozyme spcificities with different donor substrates, tamarind xyloglucan-derived oligosacchrides, overview. AtXTH13 catalyzes the release of XGO1 (Glc4-based xylogluco-oligosaccharides) and XGO3 (Glc12-based xyloglucooligosaccharides) from a mixture of minimal XET/XEH substrates, XGO2 (Glc8-based xylogluco-oligosaccharides), derived from tamarind seed xyloglucan and bearing naturally variable galactosylation. The rate of release of XGO1 is identical to that of XGO3 at all substrate concentrations across a wide range
-
-
-
additional information
?
-
O80803, Q9FKL8, Q9FKL9, Q9M0D1, Q9M0D2
XTH isozyme specificity with different donor substrates, tamarind xyloglucan-derived oligosaccharides, overview
-
-
-
additional information
?
-
B1P1S7, -, P93671, P93672
xyloglucan xyloglucosyl transferases act as xyloglucan endotransglycosylases and use a disproportionation reaction mechanism, tamarind xyloglucan is used as a donor substrate
-
-
-
additional information
?
-
B1P1S7, -, P93671, P93672
xyloglucan xyloglucosyl transferases act as xyloglucan endotransglycosylases and use a disproportionation reaction mechanism, tamarind xyloglucan is used as a donor substrate and [Xylp(alpha1,6)]-Glcp(beta1,4)-[Xylp(alpha1,6)]-Glcp(beta1,4)-[Xylp(alpha1,6)]-Glcp(beta1,4)-Glcp as acceptor substrate
-
-
-
additional information
?
-
B1P1S7, -, P93671, P93672
xyloglucan xyloglucosyl transferases act as xyloglucan endotransglycosylases and use a disproportionation reaction mechanism, tamarind xyloglucan is used as a donor substrate. Transglycosylation reactions are catalysed by HvXET6 during a two-stage reaction cycle
-
-
-
additional information
?
-
Pinus parviflora Glauca, Trigonella caerulea Ser., Pharbitis purpurea Bojer, Beckmannia syzigachne Fernald, Nicotiana tabacum SR-1, Cedrus atlantica Carr., Crocus vernus Hill
-
not: cellobiose-SR
-
-
-
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
(Substrate)
LITERATURE
(Substrate)
COMMENTARY
(Product)
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
donor xyloglucan + acceptor xyloglucan
?
show the reaction diagram
Q38909
-
-
-
?
donor xyloglucan + xyloglucan acceptor
?
show the reaction diagram
-
-
-
-
-
donor xyloglucan + xyloglucan acceptor
?
show the reaction diagram
-
-
-
-
-
donor xyloglucan + xyloglucan acceptor
?
show the reaction diagram
-
-
-
-
-
donor xyloglucan + xyloglucan acceptor
?
show the reaction diagram
-
-
-
-
-
donor xyloglucan + xyloglucan acceptor
?
show the reaction diagram
-
-
-
-
-
donor xyloglucan + xyloglucan acceptor
?
show the reaction diagram
-
Arabidopsis MERI-5 and TCH4 and cauliflower isoenzymes expression occur in dense cytoplasmic tissues predominantly involved in the assembly of new cell walls and thus in the integration of newly secreted xyloglucan into the cell wall
-
-
-
donor xyloglucan + xyloglucan acceptor
?
show the reaction diagram
-
the xyloglucan:xyloglucosyl transferase gene TCH4 is strongly upregulated by environmental stimuli, enzyme may function in modifying cell walls to allow growth, airspace formation, the development of vasculature, and reinforcement of regions under mechanical strain, TCH4 protein may contribute to the adaptive changes in morphogenesis that occurs in the organism following exposure to mechanical stimuli
-
-
-
donor xyloglucan + xyloglucan acceptor
?
show the reaction diagram
-
2 isoenzymes: sXET from seeds plays a role in degrading xyloglucan reserves in seeds during germination, eXET from epicotyls is engaged in cell wall rearrangement and integration of new xyloglucan molecules into the preexisting cell wall structure during growth
-
-
-
donor xyloglucan + xyloglucan acceptor
?
show the reaction diagram
Q8GZD5
multifunctional role in cell wall construction, role in restructuring primary cell walls at the time when secondary wall layers are deposited, probably creating and reinforcing the connections between the primary and secondary wall layers
-
-
-
donor xyloglucan + xyloglucan acceptor
?
show the reaction diagram
-, Q9ZSU4
XETs encoded by a gene family may influence plant growth and development, low pH would limit XET function in vivo
-
-
-
donor xyloglucan + xyloglucan acceptor
?
show the reaction diagram
-
role in elongation-growth and other processes involving xyloglucan metabolism
-
-
-
donor xyloglucan + xyloglucan acceptor
?
show the reaction diagram
-
enzyme is unlikely to play a role in acid-induced wall loosening but may function in cold acclimation or cold-tolerant growth, TCH4 expression is rapidly regulated by mechanical stimuli, temperature shifts, light and hormones, it may be involved in assembling nascent cell walls or reinforcing existing and expanding walls
-
-
-
donor xyloglucan + xyloglucan acceptor
?
show the reaction diagram
P93349
NtXET-1 is involved in the incorporation of small xyloglucan molecules into the cell wall by transglycosylation, role during differentation and growth of the vascular tissue, reduced NtXET-1 expression and increase in the MW of xyloglucans in older leaves might be associated with strengthening of cell walls by reduced turnover and hydrolysis of xyloglucan, control of NtXET-1 expression in leaves
-
-
-
donor xyloglucan + xyloglucan acceptor
?
show the reaction diagram
-
activity increases markedly at the exponential growth and decreases immediately at the stationary phase of cells in presence of 2,4-dichlorophenoxyacetic acid, the activity is developmentally regulated during growth but is not directly induced by plant hormones
-
-
-
donor xyloglucan + xyloglucan acceptor
?
show the reaction diagram
-
important component of cell wall metabolism, particularly expanding tissue and ripening fruits, study on the role in tomato fruit ripening and vegetative growth, tXET-B1 may incorporate new xyloglucan into the existing network
-
-
-
donor xyloglucan + xyloglucan acceptor
?
show the reaction diagram
-
regulation of XET activity: enzyme exists in plant cell walls in a transiently latent state as covalent glycosyl-enzyme complex and is active only when suitable glycosyl acceptors become available
-
-
-
donor xyloglucan + xyloglucan acceptor
?
show the reaction diagram
-
existence of different classes of XET with differing roles in vivo
-
-
-
donor xyloglucan + xyloglucan acceptor
?
show the reaction diagram
-
existence of different classes of XET with differing roles in vivo
-
-
-
donor xyloglucan + xyloglucan acceptor
?
show the reaction diagram
-
existence of different classes of XET with differing roles in vivo
-
-
-
donor xyloglucan + xyloglucan acceptor
?
show the reaction diagram
-
existence of different classes of XET with differing roles in vivo
-
-
-
donor xyloglucan + xyloglucan acceptor
?
show the reaction diagram
-
XET and xyloglucan may play a role in the cell wall changes that accompany fruit softening during ripening, substrates for XET action are located in the cell wall
-
-
-
donor xyloglucan + xyloglucan acceptor
?
show the reaction diagram
-
role in cell elongation
-
-
-
donor xyloglucan + xyloglucan acceptor
?
show the reaction diagram
-
enzyme is capable of splitting and reconnecting xyloglucan molecules in rapidly growing plant tissues, expression and presumed physiological roles of At-XTH22 and 24
-
-
-
donor xyloglucan + xyloglucan acceptor
?
show the reaction diagram
Q40144, -
enzyme mediates cell wall disassembly associated with expansive growth
-
-
-
donor xyloglucan + xyloglucan acceptor
?
show the reaction diagram
-
key enzyme responsible for forming and rearranging the cellulose/xyloglucan network of the cell wall, commitment to the construction of both cell plate and cell wall
-
-
-
donor xyloglucan + xyloglucan acceptor
?
show the reaction diagram
-
enzyme depolymerises xyloglucan
-
-
-
donor xyloglucan + xyloglucan acceptor
?
show the reaction diagram
-
role in wall assembly as well as loosening
-
-
-
donor xyloglucan + xyloglucan acceptor
?
show the reaction diagram
-
enzyme is involved in the post-germinative mobilization of xyloglucan storage reserves, involved in cell wall loosening, activity is primarily regulated at the level of gene expression
-
-
-
donor xyloglucan + xyloglucan acceptor
?
show the reaction diagram
-
enzyme is responsible for cutting and rejoining intermicrofibrillar xyloglucan chains and thus causes the wall-loosening required for plant cell expansion and plant growth, in vivo the usual acceptor is polymeric wall-bound xyloglucan
-
-
-
donor xyloglucan + xyloglucan acceptor
?
show the reaction diagram
-
reconnecting enzyme for xyloglucans, involved in the interweaving or reconstruction of cell wall matrix, which is responsible for chemical creepage that leads to morphological changes in the cell wall, responsible for cell wall loosening and integration of cell wall architecture
-
-
-
donor xyloglucan + xyloglucan acceptor
?
show the reaction diagram
-
Arabidopsis EXGT and mung bean isoenzymes expression in tissues involved in the loosening of existing cell wall material necessary for rapid cell expansion, e.g. vacuolation
-
-
-
donor xyloglucan + xyloglucan acceptor
?
show the reaction diagram
-
lentil and nasturtium, NXG1, seed enzymes are involved in the mobilization of cotyledonary xyloglucan reserves after germination
-
-
-
donor xyloglucan + xyloglucan acceptor
?
show the reaction diagram
-
involved in xyloglucan metabolism
-
-
-
donor xyloglucan + xyloglucan acceptor
?
show the reaction diagram
-
one of four enzymes, which act in concert to catalyze the mobilisation of storage xyloglucan from the cell wall
-
-
-
donor xyloglucan + xyloglucan acceptor
?
show the reaction diagram
-
XET1 may play a role in cell-wall xyloglucan metabolism, such as the incorporation of newly synthesized xyloglucan into the expanding primary cell wall or the modification of xyloglucan polymers forming cross-links between cellulose microfibrils, NXG1 in vivo predominantly exhibits xyloglucanase activity and mobilizes nonfucosylated xyloglucan seed storage reserves
-
-
-
donor xyloglucan + xyloglucan acceptor
?
show the reaction diagram
-
major role is the integration of new xyloglucan into the cell walls of the densely cytoplasmic florets
-
-
-
donor xyloglucan + xyloglucan acceptor
?
show the reaction diagram
-
associated with primary cell wall metabolism, rather than mobilisation of any seed storage xyloglucan, major role is the re-structuring of existing wall material in the rapidly vacuolating shoots
-
-
-
donor xyloglucan + xyloglucan acceptor
?
show the reaction diagram
-
one of the key enzymes involved in the breakdown of reserve xyloglucan in seeds of some dicotyledonous plants during germination
-
-
-
donor xyloglucan + xyloglucan acceptor
?
show the reaction diagram
Pinus parviflora Glauca, Trigonella caerulea Ser., Pharbitis purpurea Bojer, Beckmannia syzigachne Fernald, Nicotiana tabacum SR-1, Cedrus atlantica Carr., Crocus vernus Hill
-
acts in root development, necessary for root hair growth
-
-
-
xyloglucan oligosaccharides + xyloglucan
?
show the reaction diagram
-
-
-
-
?
xyloglucan oligosaccharides + xyloglucan
? + xyloglucan
show the reaction diagram
-
xyloglucans with MW of about 600-1000 kDa
MW about 50 kDa
-
?
additional information
?
-
-
free oligosaccharides are probably not the usual acceptor substrates in vivo
-
-
-
additional information
?
-
-
acidic polymers, probably acidic arabinogalactan proteins, and/or other apoplastic ions are naturally occurring regulators of the enzyme activity in vivo, and may thus control cell wall assembly, loosening, and growth
-
-
-
additional information
?
-
-
key enzyme in all plant processes that require cell wall remodeling
-
-
-
additional information
?
-
-
xyloglucan oligosaccharides cause cell wall loosening by enhancing xyloglucan oligosaccharide degrading enzyme activity
-
-
-
additional information
?
-
-
breaks a beta-(1->4) bond in the backbone of a xyloglucan and transfers the xyloglucanyl segment on to O-4 of the non-reducing terminal glucose residue of an acceptor, which can be a xyloglucan or an oligosaccharide of xyloglucan
-
-
-
additional information
?
-
Q5JZX2, -
breaks a beta-(1->4) bond in the backbone of a xyloglucan and transfers the xyloglucanyl segment on to O-4 of the non-reducing terminal glucose residue of an acceptor, which can be a xyloglucan or an oligosaccharide of xyloglucan
-
-
-
additional information
?
-
-
breaks a beta-(1->4) bond in the backbone of a xyloglucan and transfers the xyloglucanyl segment on to O-4 of the non-reducing terminal glucose residue of an acceptor, which can be a xyloglucan or an oligosaccharide of xyloglucan
-
-
-
additional information
?
-
Q40144, Q43527, Q43528, Q6RHX7, Q6RHX8, Q6RHX9, Q6RHY0, Q6RHY1, Q9FR51, Q9FZ05, Q9SDX0, Q9SLN9
breaks a beta-(1->4) bond in the backbone of a xyloglucan and transfers the xyloglucanyl segment on to O-4 of the non-reducing terminal glucose residue of an acceptor, which can be a xyloglucan or an oligosaccharide of xyloglucan
-
-
-
additional information
?
-
-
breaks a beta-(1->4) bond in the backbone of a xyloglucan and transfers the xyloglucanyl segment on to O-4 of the non-reducing terminal glucose residue of an acceptor, which can be a xyloglucan or an oligosaccharide of xyloglucan
-
-
-
additional information
?
-
Q9ZV40
breaks a beta-(1->4) bond in the backbone of a xyloglucan and transfers the xyloglucanyl segment on to O-4 of the non-reducing terminal glucose residue of an acceptor, which can be a xyloglucan or an oligosaccharide of xyloglucan
-
-
-
additional information
?
-
-
breaks a beta-(1->4) bond in the backbone of a xyloglucan and transfers the xyloglucanyl segment on to O-4 of the non-reducing terminal glucose residue of an acceptor, which can be a xyloglucan or an oligosaccharide of xyloglucan
-
-
-
additional information
?
-
Q1XD17, -
breaks a beta-(1->4) bond in the backbone of a xyloglucan and transfers the xyloglucanyl segment on to O-4 of the non-reducing terminal glucose residue of an acceptor, which can be a xyloglucan or an oligosaccharide of xyloglucan
-
-
-
additional information
?
-
-
breaks a beta-(1-4) bond in the backbone of a xyloglucan and transfers the xyloglucanyl segment on to O-4 of the non-reducing terminal glucose residue of an acceptor, which can be a xyloglucan or an oligosaccharide of xyloglucan
-
-
-
additional information
?
-
O80803, Q9FKL8, Q9FKL9, Q9M0D1, Q9M0D2
root-specific isozyme AtXTH1 exhibits only the endotransglucosylase activity, XET, EC 2.4.1.207, towards xyloglucan and non-detectable endohydrolytic activity, XEH, EC 3.2.1.151. Its endotransglucosylase activity is preferentially directed towards xyloglucan and water-soluble cellulose acetate, rather than to mixed-linkage beta-glucan
-
-
-
additional information
?
-
O80803, Q9FKL8, Q9FKL9, Q9M0D1, Q9M0D2
root-specific isozyme AtXTH13 exhibits only the endotransglucosylase activity, XET, EC 2.4.1.207, towards xyloglucan and non-detectable endohydrolytic activity, XEH, EC 3.2.1.151. The endotransglucosylase activity is preferentially directed towards xyloglucan and water-soluble cellulose acetate, rather than to mixed-linkage beta-glucan
-
-
-
additional information
?
-
O80803, Q9FKL8, Q9FKL9, Q9M0D1, Q9M0D2
root-specific isozyme AtXTH17 exhibits only the endotransglucosylase activity, XET, EC 2.4.1.207, towards xyloglucan and non-detectable endohydrolytic activity, XEH, EC 3.2.1.151. Its endotransglucosylase activity is preferentially directed towards xyloglucan and water-soluble cellulose acetate, rather than to mixed-linkage beta-glucan
-
-
-
additional information
?
-
O80803, Q9FKL8, Q9FKL9, Q9M0D1, Q9M0D2
root-specific isozyme AtXTH18 exhibits only the endotransglucosylase activity, XET, EC 2.4.1.207, towards xyloglucan and non-detectable endohydrolytic activity, XEH, EC 3.2.1.151. Its endotransglucosylase activity is preferentially directed towards xyloglucan and water-soluble cellulose acetate, rather than to mixed-linkage beta-glucan
-
-
-
additional information
?
-
O80803, Q9FKL8, Q9FKL9, Q9M0D1, Q9M0D2
root-specific isozyme AtXTH19 exhibits only the endotransglucosylase activity, XET, EC 2.4.1.207, towards xyloglucan and non-detectable endohydrolytic activity, XEH, EC 3.2.1.151. Its endotransglucosylase activity is preferentially directed towards xyloglucan and water-soluble cellulose acetate, rather than to mixed-linkage beta-glucan
-
-
-
additional information
?
-
-
XET activity catalyses the endo-cleavage of xyloglucan (the donor substrate) and the creation of a glycosidic link between the newly formed potentially reducing terminus and the nonreducing terminus of another xyloglucan (or xyloglucan oligosaccharide) molecule (the acceptor substrate), cleavage specificity, overview
-
-
-
METALS and IONS
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
Al3+
-
best as chloride at lower concentrations around 0.1 mM, inhibitory at higher concentrations
Ca2+
-
10 mM, slightly activates
Ca2+
-
best as chloride at lower concentrations around 0.1 mM, inhibitory at higher concentrations
Ca2+
-
stimulates activity by 7-8% at 5-15 mM
Ca2+
-
55% reduced activity at 1 mM
K+
-
best at about 300 mM
La3+
-
best as chloride at lower concentrations around 0.1 mM, inhibitory at higher concentrations
Mg2+
-
10 mM, slightly activates
Mg2+
-
best as chloride at lower concentrations around 0.1 mM, inhibitory at higher concentrations
Na+
-
best at about 300 mM
NH4+
-
best at about 300 mM
Mn2+
-
10 mM, slightly activates
additional information
-
TCH4 protein is not activated by Ca+ or other divalent cations
additional information
-
the enzyme is activated by a wide variety of inorganic and organic salts
additional information
-
NaCl has hardly any effect on the activity
INHIBITORS
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
IMAGE
(NH4)2SO4
-
1 M, about 50% inhibition
4-O-methyl-glucuronoxylan
-
-
-
Ag+
-
1 mM; partial inhibition
AlCl3
-
inhibitory at concentrations above 0.1 mM
alginate
-
alpha-carrageenan from red algae Gigartina ssp.
CaCl2
-
300 mM, about 50% inhibition
CaCl2
-
inhibitory at concentrations above 0.1 mM
ethanol
-
15%, about 3 M, about 50% inhibition of product formation
H2O2
-
1 M, about 50% inhibition
Hg2+
-
1 mM, partial inhibition
Hg2+
-
at least 10 mM
homogalacturonan
-
-
-
La3+
-
10 mM, partial inhibition
LaCl3
-
inhibtory at 5 mM
Mg2+
-
inhibits activity by 3-4% at 5-15 mM
NaCl
-
1 M, about 50% inhibition
NaN3
-
1 M, about 50% inhibition
phosphate
-
300 mM, about 50% inhibition
Pyridine
-
300 mM, about 50% inhibition
Xylalpha(1-6)Glcbeta(1-4)(Galbeta(1-2)Xylalpha(1-6))Glcbeta(1-4)(Galbeta(1-2)Xylalpha(1-6))Glcbeta(1-4)Glc-ol
-, Q9ZSU4
significant substrate inhibition of isoenzyme XTR9 at high concentrations; slight substrate inhibition of isoenzymes Meri-5 and EXGT at high concentrations
xyloglucan-derived oligosaccharide
-
-
Zn2+
-
10 mM, partial inhibition
MgCl2
-
inhibitory at concentrations above 0.1 mM
additional information
-
not inhibited by 10 mM D-gluconolactone, chelating agents, cello-oligosaccharides, cellopentaose
-
additional information
-
not inhibited by 1 M fluoride, 1 M urea, 1 M DMSO, 300 mM borate, 100 mM EDTA, 100 mM Triton X-100
-
additional information
-, Q6YDN9
glycosylation is required for activity, deglycosylation results in 40% loss of activity
-
additional information
Q40144, Q43527, Q43528, Q6RHX7, Q6RHX8, Q6RHX9, Q6RHY0, Q6RHY1, Q9FR51, Q9FZ05, Q9SDX0, Q9SLN9
the expression of SIXTHs and the activity of xyloglucan endotransglucosylase can be modulated in tomatoes infected by Penicillium expansum; the expression of SIXTHs and the activity of xyloglucan endotransglucosylase can be modulated in tomatoes infected by Penicillium expansum; the expression of SIXTHs and the activity of xyloglucan endotransglucosylase can be modulated in tomatoes infected by Penicillium expansum; the expression of SIXTHs and the activity of xyloglucan endotransglucosylase can be modulated in tomatoes infected by Penicillium expansum; the expression of SIXTHs and the activity of xyloglucan endotransglucosylase can be modulated in tomatoes infected by Penicillium expansum; the expression of SIXTHs and the activity of xyloglucan endotransglucosylase can be modulated in tomatoes infected by Penicillium expansum; the expression of SIXTHs and the activity of xyloglucan endotransglucosylase can be modulated in tomatoes infected by Penicillium expansum; the expression of SIXTHs and the activity of xyloglucan endotransglucosylase can be modulated in tomatoes infected by Penicillium expansum; the expression of SIXTHs and the activity of xyloglucan endotransglucosylase can be modulated in tomatoes infected by Penicillium expansum; the expression of SIXTHs and the activity of xyloglucan endotransglucosylase can be modulated in tomatoes infected by Penicillium expansum; the expression of SIXTHs and the activity of xyloglucan endotransglucosylase can be modulated in tomatoes infected by Penicillium expansum; the expression of SIXTHs and the activity of xyloglucan endotransglucosylase can be modulated in tomatoes infected by Penicillium expansum
-
ACTIVATING COMPOUND
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
IMAGE
2-mercaptoethanol
-
slightly activates
acidic arabinogalactan protein
-
MW about 5000 Da, acidic polymers and/or other apoplastic ions are naturally occurring regulators of the enzyme activity in vivo, and may thus control cell wall assembly, loosening, and growth, composition overview
-
alpha-naphthalene acetic acid
-
-
ascorbate
-
10 mM, slightly activates
Bovine serum albumin
-
enhances activity by stabilizing TCH4 protein conformation
-
dithiothreitol
-
30% increase of activity at 1 mM
gibberellin
Q5JZX2, -
-
Gum arabic
-
from Acacia sp., 0.02-0.4% w/v, activation of desalted enzyme, activation is increased in presence of suboptimal concentration of NaCl
-
Pectin
-
from Citrus sp., 0.02-0.4% w/v, activation of desalted enzyme, activation is increased in presence of suboptimal concentration of NaCl
-
spermidine
-
1 mM, slighly activates
Urea
-
enhances activity of TCH4 protein
xyloglucan oligosaccharides
-
xyloglucan oligosaccharides enhance enzyme activity
xyloglucan-derived oligosaccharide
-
stimulates, highest stimulating effect with the nonasaccharide Glc4Xyl3Gal2, lowest one with the pentasaccharide Glc3Xyl2
hypochlorite-oxidized xyloglucan
-
from tamarind, 0.02-0.4% w/v, activation of desalted enzyme, activation is increased in presence of suboptimal concentration of NaCl
-
additional information
Q40144
LeEXT gene encoding XET is induced by hormone treatments that elicites elongation of hypocotyl segments, e.g. treatment with auxin, brassinolide or 2,4-D
-
additional information
-
TCH4 protein is not activated by reducing agents, spermidine, spermine, glycerol or carboxymethylcellulose
-
additional information
-
strong positive correlation between gibberellic acid-enhanced length and extractable XET activity in internodes
-
additional information
-
At-XTH22 is strongly induced in response to mechanical perturbations such as wind or touch
-
additional information
-
-
-
additional information
-, Q6YDN9
glycosylation is required for activity, deglycosylation results in 40% loss of activity
-
additional information
-
no activation by carboxymethylcellulose, non-oxidized xyloglucan, homo-galacturonic acid, and starch, overview
-
KM VALUE [mM]
KM VALUE [mM] Maximum
SUBSTRATE
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
IMAGE
0.062
-
nonasaccharide XLLGol
-
pH 5.5, 25C, recombinant N93S mutant enzyme
-
0.075
-
nonasaccharide XLLGol
-
pH 5.5, 25C, recombinant wild-type enzyme
-
0.0085
-
reduced xyloglucan-derived heptasaccharide
B1P1S7, P93668
recombinant isozyme XET6, at 30C in 100 mM sodium succinate buffer, pH 6.0, containing 5 mM CaCl2
-
0.0399
-
reduced xyloglucan-derived heptasaccharide
B1P1S7, P93668
purified isozyme XET5, at 30C in 100 mM sodium succinate buffer, pH 6.0, containing 5 mM CaCl2
-
0.017
-
rhodamin-conjugated Glc3-Xyl3-Gal2-glucitol
-
isozyme eXET, pH 5.5, 30C
-
0.131
-
rhodamin-conjugated Glc3-Xyl3-Gal2-glucitol
-
isozyme sXET, pH 5.5, 30C
-
0.05
-
Xyl(1-6)Glc(1-4)(Xyl(1-6))Glc(1-4)(Fuc-Gal-Xyl(1-6))Glc(1-4)Glc
-
xyloglucan-derived nonasaccharide
0.05
-
Xyl(1-6)Glc(1-4)(Xyl(1-6))Glc(1-4)(Fuc-Gal-Xyl(1-6))Glc(1-4)Glc
-
-
0.2
-
Xyl(1-6)Glc(1-4)(Xyl(1-6))Glc(1-4)Glc
-
-
0.019
-
Xylalpha(1-6)Glcbeta(1-4)(Galbeta(1-2)Xylalpha(1-6))Glcbeta(1-4)(Galbeta(1-2)Xylalpha(1-6))Glcbeta(1-4)Glc
-
-
0.005
-
Xylalpha(1-6)Glcbeta(1-4)(Galbeta(1-2)Xylalpha(1-6))Glcbeta(1-4)(Galbeta(1-2)Xylalpha(1-6))Glcbeta(1-4)Glc-ol
-, Q9ZSU4
isoenzyme XTR9
0.016
-
Xylalpha(1-6)Glcbeta(1-4)(Galbeta(1-2)Xylalpha(1-6))Glcbeta(1-4)(Galbeta(1-2)Xylalpha(1-6))Glcbeta(1-4)Glc-ol
-
isoenzyme M55a
0.017
-
Xylalpha(1-6)Glcbeta(1-4)(Galbeta(1-2)Xylalpha(1-6))Glcbeta(1-4)(Galbeta(1-2)Xylalpha(1-6))Glcbeta(1-4)Glc-ol
-, Q9ZSU4
isoenzyme TCH4
0.028
-
Xylalpha(1-6)Glcbeta(1-4)(Galbeta(1-2)Xylalpha(1-6))Glcbeta(1-4)(Galbeta(1-2)Xylalpha(1-6))Glcbeta(1-4)Glc-ol
-
isoenzyme M45
0.03
-
Xylalpha(1-6)Glcbeta(1-4)(Galbeta(1-2)Xylalpha(1-6))Glcbeta(1-4)(Galbeta(1-2)Xylalpha(1-6))Glcbeta(1-4)Glc-ol
-, Q9ZSU4
isoenzyme EXGT
0.031
0.035
Xylalpha(1-6)Glcbeta(1-4)(Galbeta(1-2)Xylalpha(1-6))Glcbeta(1-4)(Galbeta(1-2)Xylalpha(1-6))Glcbeta(1-4)Glc-ol
-
isoenzymes M35a and M35b
0.032
-
Xylalpha(1-6)Glcbeta(1-4)(Galbeta(1-2)Xylalpha(1-6))Glcbeta(1-4)(Galbeta(1-2)Xylalpha(1-6))Glcbeta(1-4)Glc-ol
-
isoenzyme M55b
0.04
-
Xylalpha(1-6)Glcbeta(1-4)(Galbeta(1-2)Xylalpha(1-6))Glcbeta(1-4)(Galbeta(1-2)Xylalpha(1-6))Glcbeta(1-4)Glc-ol
-, Q9ZSU4
isoenzyme Meri-5
0.071
0.082
Xylalpha(1-6)Glcbeta(1-4)(Galbeta(1-2)Xylalpha(1-6))Glcbeta(1-4)(Galbeta(1-2)Xylalpha(1-6))Glcbeta(1-4)Glc-ol
-
isoenzymes C45a and C45b
0.073
-
Xylalpha(1-6)Glcbeta(1-4)(Galbeta(1-2)Xylalpha(1-6))Glcbeta(1-4)(Galbeta(1-2)Xylalpha(1-6))Glcbeta(1-4)Glc-ol
-
acceptor XLLG-ol, recombinant TCH4 protein
0.073
-
Xylalpha(1-6)Glcbeta(1-4)(Galbeta(1-2)Xylalpha(1-6))Glcbeta(1-4)(Galbeta(1-2)Xylalpha(1-6))Glcbeta(1-4)Glc-ol
-
recombinant At-XTH22
0.13
-
Xylalpha(1-6)Glcbeta(1-4)(Galbeta(1-2)Xylalpha(1-6))Glcbeta(1-4)(Galbeta(1-2)Xylalpha(1-6))Glcbeta(1-4)Glc-ol
-
isoenzyme C30
0.033
-
Xylalpha(1-6)Glcbeta(1-4)(Xylalpha(1-6))Glcbeta(1-4)(Xylalpha(1-6))Glcbeta(1-4)Glc
-
-
0.1
-
Xylalpha(1-6)Glcbeta(1-4)(Xylalpha(1-6))Glcbeta(1-4)(Xylalpha(1-6))Glcbeta(1-4)Glc-ol
-
reduced xyloglucan-derived heptasaccharide XXXG-ol, derived from kiwifruit xyloglucan
0.32
-
Xylalpha(1-6)Glcbeta(1-4)(Xylalpha(1-6))Glcbeta(1-4)(Xylalpha(1-6))Glcbeta(1-4)Glc-ol
-
XXXG-ol
0.23
-
Xylalpha(1-6)Glcbeta(1-4)(Xylalpha(1-6))Glcbeta(1-4)(Xylalpha(1-6))Glcbeta(1-4)Glcbeta(1-4)(Xylalpha(1-6))Glcbeta(1-4)(Xylalpha(1-6))Glcbeta(1-4)(Xylalpha(1-6))Glcbeta(1-4)Glc-ol
-
XXXGXXXG-ol
1.4
-
xyloglucan
-
pH 5.5, 25C, recombinant N93S mutant enzyme
2.2
-
xyloglucan
-
pH 5.5, 25C, recombinant wild-type enzyme
0.0003
-
xyloglucan acceptor
-
recombinant TCH4 protein
0.0003
-
xyloglucan acceptor
-
At-XTH22, high MW xyloglucan
0.0053
-
[Xylp(alpha1,6)]-Glcp(beta1,4)-[Galp(beta1,2)-Xylp(alpha1,6)]-Glcp(beta1,4)-[Galp(beta1,2)-Xylp(alpha1,6)]-Glcp(beta1,4)-Glcp
B1P1S7, P93671, P93672
isoform XET6, using xyloglycan as donor substrate, at 30C, in 50 mM ammonium acetate buffer pH 6.0
0.039
-
[Xylp(alpha1,6)]-Glcp(beta1,4)-[Galp(beta1,2)-Xylp(alpha1,6)]-Glcp(beta1,4)-[Galp(beta1,2)-Xylp(alpha1,6)]-Glcp(beta1,4)-Glcp
B1P1S7, P93671, P93672
isoform XET3, using xyloglycan as donor substrate, at 30C, in 50 mM ammonium acetate buffer pH 6.0
0.04
-
[Xylp(alpha1,6)]-Glcp(beta1,4)-[Galp(beta1,2)-Xylp(alpha1,6)]-Glcp(beta1,4)-[Galp(beta1,2)-Xylp(alpha1,6)]-Glcp(beta1,4)-Glcp
B1P1S7, P93671, P93672
isoform XET4, using xyloglycan as donor substrate, at 30C, in 50 mM ammonium acetate buffer pH 6.0
0.0086
-
[Xylp(alpha1,6)]-Glcp(beta1,4)-[Xylp(alpha1,6)]-Glcp(beta1,4)-[Galp(beta1,2)-Xylp(alpha1,6)]-Glcp(beta1,4)-Glcp/[Xylp(alpha1,6)]-Glcp(beta1,4)-[Galp(beta1,2)-Xylp(alpha1,6)]-Glcp(beta1,4)-[Xylp(alpha1,6)]-Glcp(beta1,4)-Glcp
B1P1S7, P93671, P93672
isoform XET6, using xyloglycan as donor substrate, at 30C, in 50 mM ammonium acetate buffer pH 6.0
-
0.0113
-
[Xylp(alpha1,6)]-Glcp(beta1,4)-[Xylp(alpha1,6)]-Glcp(beta1,4)-[Galp(beta1,2)-Xylp(alpha1,6)]-Glcp(beta1,4)-Glcp/[Xylp(alpha1,6)]-Glcp(beta1,4)-[Galp(beta1,2)-Xylp(alpha1,6)]-Glcp(beta1,4)-[Xylp(alpha1,6)]-Glcp(beta1,4)-Glcp
B1P1S7, P93671, P93672
isoform XET3, using xyloglycan as donor substrate, at 30C, in 50 mM ammonium acetate buffer pH 6.0
-
0.0242
-
[Xylp(alpha1,6)]-Glcp(beta1,4)-[Xylp(alpha1,6)]-Glcp(beta1,4)-[Galp(beta1,2)-Xylp(alpha1,6)]-Glcp(beta1,4)-Glcp/[Xylp(alpha1,6)]-Glcp(beta1,4)-[Galp(beta1,2)-Xylp(alpha1,6)]-Glcp(beta1,4)-[Xylp(alpha1,6)]-Glcp(beta1,4)-Glcp
B1P1S7, P93671, P93672
isoform XET4, using xyloglycan as donor substrate, at 30C, in 50 mM ammonium acetate buffer pH 6.0
-
0.005
-
[Xylp(alpha1,6)]-Glcp(beta1,4)-[Xylp(alpha1,6)]-Glcp(beta1,4)-[Xylp(alpha1,6)]-Glcp(beta1,4)-Glcp
B1P1S7, P93671, P93672
isoform XET4, using xyloglycan as donor substrate, at 30C, in 50 mM ammonium acetate buffer pH 6.0
0.0103
-
[Xylp(alpha1,6)]-Glcp(beta1,4)-[Xylp(alpha1,6)]-Glcp(beta1,4)-[Xylp(alpha1,6)]-Glcp(beta1,4)-Glcp
B1P1S7, P93671, P93672
isoform XET3, using xyloglycan as donor substrate, at 30C, in 50 mM ammonium acetate buffer pH 6.0
0.0106
-
[Xylp(alpha1,6)]-Glcp(beta1,4)-[Xylp(alpha1,6)]-Glcp(beta1,4)-[Xylp(alpha1,6)]-Glcp(beta1,4)-Glcp
B1P1S7, P93671, P93672
isoform XET6, using xyloglycan as donor substrate, at 30C, in 50 mM ammonium acetate buffer pH 6.0
1.6
-
Glc8-basedxyloglucan oligosaccharide
B1P1S7, P93668
recombinant isozyme XET6, at 30C in 100 mM sodium succinate buffer, pH 6.0, containing 5 mM CaCl2
-
additional information
-
additional information
-
Km: 0.6 mg/ml for kiwifruit xyloglucan
-
additional information
-
additional information
-
recombinant TCH4 protein, Km: 0.62 mg/ml for nonfucosylated tamarind xyloglucan as donor, 1.8 mg/ml for fucosylated pea xyloglucan
-
additional information
-
additional information
-
-
-
additional information
-
additional information
-
recombinant TCH4 protein, Km: 0.62 mg/ml for nonfucosylated tamarind xyloglucan as donor, 1.8 mg/ml for fucosylated pea xyloglucan
-
additional information
-
additional information
Q9ZV40
purified AtXTH21 activity, Km: 0.6245 mg/ml
-
additional information
-
additional information
O80803, Q9FKL8, Q9FKL9, Q9M0D1, Q9M0D2
Michaelis-Menten kinetics for AtXTH12, overviews; Michaelis-Menten kinetics for AtXTH13, overviews
-
TURNOVER NUMBER [1/s]
TURNOVER NUMBER MAXIMUM[1/s]
SUBSTRATE
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
IMAGE
4.8
-
Gal(beta1-4)Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)Glc
-
-
0.139
-
Glc8-based xyloglucan oligosaccharide
B1P1S7, P93668
recombinant isozyme XET6, at 30C in 100 mM sodium succinate buffer, pH 6.0, containing 5 mM CaCl2
-
0.00031
-
pea xyloglucan
-
recombinant TCH4 protein, with XLLG-ol as acceptor
-
0.0024
-
reduced xyloglucan-derived heptasaccharide
B1P1S7, P93668
purified isozyme XET5, at 30C in 100 mM sodium succinate buffer, pH 6.0, containing 5 mM CaCl2
-
0.0089
-
reduced xyloglucan-derived heptasaccharide
B1P1S7, P93668
recombinant isozyme XET6, at 30C in 100 mM sodium succinate buffer, pH 6.0, containing 5 mM CaCl2
-
0.00018
-
tamarind xyloglucan
-
recombinant TCH4 protein, with XLLG-ol as acceptor
-
0.45
-
[Xyl(alpha1-6)]Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)[Xyl(alpha1-6)]Glc(beta1-4)Glc
-
-
0.007
-
[Xylp(alpha1,6)]-Glcp(beta1,4)-[Galp(beta1,2)-Xylp(alpha1,6)]-Glcp(beta1,4)-[Galp(beta1,2)-Xylp(alpha1,6)]-Glcp(beta1,4)-Glcp
B1P1S7, P93671, P93672
isoform XET3, using xyloglycan as donor substrate, at 30C, in 50 mM ammonium acetate buffer pH 6.0
0.021
-
[Xylp(alpha1,6)]-Glcp(beta1,4)-[Galp(beta1,2)-Xylp(alpha1,6)]-Glcp(beta1,4)-[Galp(beta1,2)-Xylp(alpha1,6)]-Glcp(beta1,4)-Glcp
B1P1S7, P93671, P93672
isoform XET4, using xyloglycan as donor substrate, at 30C, in 50 mM ammonium acetate buffer pH 6.0
0.17
-
[Xylp(alpha1,6)]-Glcp(beta1,4)-[Galp(beta1,2)-Xylp(alpha1,6)]-Glcp(beta1,4)-[Galp(beta1,2)-Xylp(alpha1,6)]-Glcp(beta1,4)-Glcp
B1P1S7, P93671, P93672
isoform XET6, using xyloglycan as donor substrate, at 30C, in 50 mM ammonium acetate buffer pH 6.0
0.005
-
[Xylp(alpha1,6)]-Glcp(beta1,4)-[Xylp(alpha1,6)]-Glcp(beta1,4)-[Galp(beta1,2)-Xylp(alpha1,6)]-Glcp(beta1,4)-Glcp/[Xylp(alpha1,6)]-Glcp(beta1,4)-[Galp(beta1,2)-Xylp(alpha1,6)]-Glcp(beta1,4)-[Xylp(alpha1,6)]-Glcp(beta1,4)-Glcp
B1P1S7, P93671, P93672
isoform XET3, using xyloglycan as donor substrate, at 30C, in 50 mM ammonium acetate buffer pH 6.0
-
0.017
-
[Xylp(alpha1,6)]-Glcp(beta1,4)-[Xylp(alpha1,6)]-Glcp(beta1,4)-[Galp(beta1,2)-Xylp(alpha1,6)]-Glcp(beta1,4)-Glcp/[Xylp(alpha1,6)]-Glcp(beta1,4)-[Galp(beta1,2)-Xylp(alpha1,6)]-Glcp(beta1,4)-[Xylp(alpha1,6)]-Glcp(beta1,4)-Glcp
B1P1S7, P93671, P93672
isoform XET4, using xyloglycan as donor substrate, at 30C, in 50 mM ammonium acetate buffer pH 6.0
-
0.12
-
[Xylp(alpha1,6)]-Glcp(beta1,4)-[Xylp(alpha1,6)]-Glcp(beta1,4)-[Galp(beta1,2)-Xylp(alpha1,6)]-Glcp(beta1,4)-Glcp/[Xylp(alpha1,6)]-Glcp(beta1,4)-[Galp(beta1,2)-Xylp(alpha1,6)]-Glcp(beta1,4)-[Xylp(alpha1,6)]-Glcp(beta1,4)-Glcp
B1P1S7, P93671, P93672
isoform XET6, using xyloglycan as donor substrate, at 30C, in 50 mM ammonium acetate buffer pH 6.0
-
0.005
-
[Xylp(alpha1,6)]-Glcp(beta1,4)-[Xylp(alpha1,6)]-Glcp(beta1,4)-[Xylp(alpha1,6)]-Glcp(beta1,4)-Glcp
B1P1S7, P93671, P93672
isoform XET3, using xyloglycan as donor substrate, at 30C, in 50 mM ammonium acetate buffer pH 6.0
0.006
-
[Xylp(alpha1,6)]-Glcp(beta1,4)-[Xylp(alpha1,6)]-Glcp(beta1,4)-[Xylp(alpha1,6)]-Glcp(beta1,4)-Glcp
B1P1S7, P93671, P93672
isoform XET4, using xyloglycan as donor substrate, at 30C, in 50 mM ammonium acetate buffer pH 6.0
0.058
-
[Xylp(alpha1,6)]-Glcp(beta1,4)-[Xylp(alpha1,6)]-Glcp(beta1,4)-[Xylp(alpha1,6)]-Glcp(beta1,4)-Glcp
B1P1S7, P93671, P93672
isoform XET6, using xyloglycan as donor substrate, at 30C, in 50 mM ammonium acetate buffer pH 6.0
kcat/KM VALUE [1/mMs-1]
kcat/KM VALUE [1/mMs-1] Maximum
SUBSTRATE
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
IMAGE
0.0869
-
Glc8-based xyloglucan oligosaccharide
B1P1S7, P93668
recombinant isozyme XET6, at 30C in 100 mM sodium succinate buffer, pH 6.0, containing 5 mM CaCl2
0
0.2
-
reduced xyloglucan-derived heptasaccharide
B1P1S7, P93668
purified isozyme XET5, at 30C in 100 mM sodium succinate buffer, pH 6.0, containing 5 mM CaCl2
0
1.1
-
reduced xyloglucan-derived heptasaccharide
B1P1S7, P93668
recombinant isozyme XET6, at 30C in 100 mM sodium succinate buffer, pH 6.0, containing 5 mM CaCl2
0
0.2
-
[Xylp(alpha1,6)]-Glcp(beta1,4)-[Galp(beta1,2)-Xylp(alpha1,6)]-Glcp(beta1,4)-[Galp(beta1,2)-Xylp(alpha1,6)]-Glcp(beta1,4)-Glcp
B1P1S7, P93671, P93672
isoform XET3, using xyloglycan as donor substrate, at 30C, in 50 mM ammonium acetate buffer pH 6.0
293161
0.5
-
[Xylp(alpha1,6)]-Glcp(beta1,4)-[Galp(beta1,2)-Xylp(alpha1,6)]-Glcp(beta1,4)-[Galp(beta1,2)-Xylp(alpha1,6)]-Glcp(beta1,4)-Glcp
B1P1S7, P93671, P93672
isoform XET4, using xyloglycan as donor substrate, at 30C, in 50 mM ammonium acetate buffer pH 6.0
293161
30
-
[Xylp(alpha1,6)]-Glcp(beta1,4)-[Galp(beta1,2)-Xylp(alpha1,6)]-Glcp(beta1,4)-[Galp(beta1,2)-Xylp(alpha1,6)]-Glcp(beta1,4)-Glcp
B1P1S7, P93671, P93672
isoform XET6, using xyloglycan as donor substrate, at 30C, in 50 mM ammonium acetate buffer pH 6.0
293161
0.4
-
[Xylp(alpha1,6)]-Glcp(beta1,4)-[Xylp(alpha1,6)]-Glcp(beta1,4)-[Galp(beta1,2)-Xylp(alpha1,6)]-Glcp(beta1,4)-Glcp/[Xylp(alpha1,6)]-Glcp(beta1,4)-[Galp(beta1,2)-Xylp(alpha1,6)]-Glcp(beta1,4)-[Xylp(alpha1,6)]-Glcp(beta1,4)-Glcp
B1P1S7, P93671, P93672
isoform XET3, using xyloglycan as donor substrate, at 30C, in 50 mM ammonium acetate buffer pH 6.0
0
1
-
[Xylp(alpha1,6)]-Glcp(beta1,4)-[Xylp(alpha1,6)]-Glcp(beta1,4)-[Galp(beta1,2)-Xylp(alpha1,6)]-Glcp(beta1,4)-Glcp/[Xylp(alpha1,6)]-Glcp(beta1,4)-[Galp(beta1,2)-Xylp(alpha1,6)]-Glcp(beta1,4)-[Xylp(alpha1,6)]-Glcp(beta1,4)-Glcp
B1P1S7, P93671, P93672
isoform XET4, using xyloglycan as donor substrate, at 30C, in 50 mM ammonium acetate buffer pH 6.0
0
13
-
[Xylp(alpha1,6)]-Glcp(beta1,4)-[Xylp(alpha1,6)]-Glcp(beta1,4)-[Galp(beta1,2)-Xylp(alpha1,6)]-Glcp(beta1,4)-Glcp/[Xylp(alpha1,6)]-Glcp(beta1,4)-[Galp(beta1,2)-Xylp(alpha1,6)]-Glcp(beta1,4)-[Xylp(alpha1,6)]-Glcp(beta1,4)-Glcp
B1P1S7, P93671, P93672
isoform XET6, using xyloglycan as donor substrate, at 30C, in 50 mM ammonium acetate buffer pH 6.0
0
0.5
-
[Xylp(alpha1,6)]-Glcp(beta1,4)-[Xylp(alpha1,6)]-Glcp(beta1,4)-[Xylp(alpha1,6)]-Glcp(beta1,4)-Glcp
B1P1S7, P93671, P93672
isoform XET3, using xyloglycan as donor substrate, at 30C, in 50 mM ammonium acetate buffer pH 6.0
293159
1
-
[Xylp(alpha1,6)]-Glcp(beta1,4)-[Xylp(alpha1,6)]-Glcp(beta1,4)-[Xylp(alpha1,6)]-Glcp(beta1,4)-Glcp
B1P1S7, P93671, P93672
isoform XET4, using xyloglycan as donor substrate, at 30C, in 50 mM ammonium acetate buffer pH 6.0
293159
5
-
[Xylp(alpha1,6)]-Glcp(beta1,4)-[Xylp(alpha1,6)]-Glcp(beta1,4)-[Xylp(alpha1,6)]-Glcp(beta1,4)-Glcp
B1P1S7, P93671, P93672
isoform XET6, using xyloglycan as donor substrate, at 30C, in 50 mM ammonium acetate buffer pH 6.0
293159
SPECIFIC ACTIVITY [µmol/min/mg]
SPECIFIC ACTIVITY MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
7.2e-08
-
-
enzyme from crude homogenate, at 30C
0.0002178
-
-
after 3025fold purification, at 30C
0.02
-
-
crude cell extract, at 30C in 100 mM succinate or ammonium acetate buffers, pH 6.0, containing 5 mM calcium chloride
0.06
-
-
pH 5.5, 25C, recombinant N93S mutant enzyme
0.072
-
B1P1S7, P93668
enzyme from crude homogenate; enzyme from crude homogenate
0.18
-
-
pH 5.5, 25C, recombinant wild-type enzyme
0.812
-
-, Q6YDN9
purified enzyme, radioactive assay method
31.07
-
B1P1S7, P93668
after 432fold purification; after 432fold purification
60.51
-
-
after 3025fold purification, at 30C in 100 mM succinate or ammonium acetate buffers, pH 6.0, containing 5 mM calcium chloride
additional information
-
-
-
additional information
-
-
a rapid, semiquantitative assay suitable for testing crude plant extracts directly on to the test paper
additional information
-
-
-
additional information
-
-
low cost, simple, high-speed colorimetric assay that allows to analyze multiple samples simultaneously
additional information
-
-, Q6YDN9
different assay methods, overview
additional information
-
-
-
additional information
-
-
after purification the concentration of enzymatically active XET is about 29 mg/l with a specific activity of 11 U/mg
pH OPTIMUM
pH MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
4.5
-
Q1XD17, -
in vivo XET assay
4.7
-
Q5JZX2, -
enzymatic assay
5
5.5
-
isoenzyme C30
5
5.5
-
isoenzymes M35a, M35b and M55b
5
6
-
isozyme eXET
5
7
-
isoenzymes M45 and M55a
5
-
O80803, Q9FKL8, Q9FKL9, Q9M0D1, Q9M0D2
AtXTH12
5.2
-
Q9ZV40
XET activity assay
5.5
8
-
isozyme sXET, broad optimum
5.5
-
-
-
5.5
-
-
-
5.5
-
-
assay at
5.5
-
-
enzyme kinetics assay
5.5
-
P24806
xyloglucan endotransglucosylase assay
6
6.5
-, Q9ZSU4
isoenzymes TCH4, Meri-5, EXGT and XTR9, EXGT shows very sharp pH-optimum at pH 6; isoenzymes TCH4, Meri-5, EXGT and XTR9, EXGT shows very sharp pH-optimum at pH 6
6
-
Q40144, Q43527, Q43528, Q6RHX7, Q6RHX8, Q6RHX9, Q6RHY0, Q6RHY1, Q9FR51, Q9FZ05, Q9SDX0, Q9SLN9
xyloglucan endotransglucosylase assay; xyloglucan endotransglucosylase assay; xyloglucan endotransglucosylase assay; xyloglucan endotransglucosylase assay; xyloglucan endotransglucosylase assay; xyloglucan endotransglucosylase assay; xyloglucan endotransglucosylase assay; xyloglucan endotransglucosylase assay; xyloglucan endotransglucosylase assay; xyloglucan endotransglucosylase assay; xyloglucan endotransglucosylase assay; xyloglucan endotransglucosylase assay
6
-
-
isozyme XTH26
6
-
O80803, Q9FKL8, Q9FKL9, Q9M0D1, Q9M0D2
AtXTH13
6
-
B1P1S7, -, P93671, P93672
assay at; assay at; assay at
6
-
P93349
assay at
6.5
-
-
isoenzymes C45a and C45b
6.5
-
-
isozyme XTH14
7
-
O80803, Q9FKL8, Q9FKL9, Q9M0D1, Q9M0D2
AtXTH19
7.2
-
O80803, Q9FKL8, Q9FKL9, Q9M0D1, Q9M0D2
AtXTH17
7.5
-
O80803, Q9FKL8, Q9FKL9, Q9M0D1, Q9M0D2
AtXTH18
additional information
-
-
pI: 5.0-5.14
pH RANGE
pH RANGE MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
4
7
-
pH 4: 25-35% of maximal activity, pH 7: 30-40% of maximal activity
4
8
-
both isoenzymes show a fast and symmetrical decrease in activity on both sides of their optima followed by an acidic shoulder between pH 4.0 and 5.0 for XTH14 and between pH 4.0 and 4.5 for XTH26. At pH 4.0 and 8.0, XTH14 retains about 30% and XTH26 40% of its maximal activity
4
8
O80803, Q9FKL8, Q9FKL9, Q9M0D1, Q9M0D2
AtXTH13 is more tolerant to basic environments, retaining 40% of its maximal activity at pH 8.0, but it displays only 20% activity at pH 4.0. The enzyme is inactive at pH 3.5. Bell-shaped curve of AtXTH13; bell-shaped curve of AtXTH12
4.5
6.5
-
pH-profile, rapid loss of activity below pH 4.5 and above pH 6.5
4.5
8
-, Q9ZSU4
TCH4 and Meri-5 retain approximately 10-15% of its maximal activity at pH 4.5 and 65% at pH 8; XTR9 retains approximately 35% of its maximal activity at pH 4.5 and 30% at pH 8
5
7
-
isozyme XTH26
5
7.5
-
pH 5: about 30% of maximal activity, pH 7.5: about 40% of maximal activity
5
8
-
active between
5.5
7
-
isozyme XTH14
5.5
8
O80803, Q9FKL8, Q9FKL9, Q9M0D1, Q9M0D2
the activity-pH profile of AtXTH17 totally shifts towards the alkali side with its optimum at pH 7.2 and with an unusual acid intolerance. AtXTH18 shows the highest acid tolerance of these three enzymes. At pH 6.0 the enzyme still displays 60% of its activity at pH 7.0, whereas the activities of AtXTH17 and AtXTH19 drop below 30% of their maximal activity at pH 6.0. At or below pH 5.5, all three enzymes display less than 20% of their maximal activities. On the basic side, AtXTH17 shows the highest tolerance with activity of more than 80% of maximal activity at pH 8.0; the activity-pH profile of AtXTH18 totally shifts towards the alkali side with optimum at pH 7.5 and with an unusual acid intolerance. At pH 6.0 the enzyme still displays 60% of its activity at pH 7.0. At or below pH 5.5, the enzyme displays less than 20% of its maximal activity. On the basic side, AtXTH18 shows the highest tolerance with activities of more than 80% of its maximal activity at pH 8.0; the activity-pH profile of AtXTH19 totally shifts towards the alkali side with optimum at pH 7.0 and with an unusual acid intolerance. Activity of AtXTH19 drops below 30% of its maximal activity at pH 6.0. At or below pH 5.5, the enzymes displays less than 20% of its maximal activities. AtXTH19 retains 90% of its maximal activity at pH 7.5, but shows a 40% activity drop between pH 7.5 and pH 8.0
additional information
-
-, Q6YDN9
pH-profile, sigmoidal decline in activity at high pH, at low pH occurs an abrupt drop in activity
additional information
-
-
isozymes sXET and eXET show different pH-profiles
TEMPERATURE OPTIMUM
TEMPERATURE OPTIMUM MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
12
18
-
recombinant TCH4 protein
18
-
-, Q9ZSU4
isoenzymes TCH4 and XTR9; isoenzymes TCH4 and XTR9
20
25
O80803, Q9FKL8, Q9FKL9, Q9M0D1, Q9M0D2
-
20
-
-
assay at
22
-
-
assay at
22
-
-
room temperature, assay at
22
-
Q1XD17, -
in vivo XET assay
22
-
Q5JZX2, -
enzymatic assay at room temperature
25
-
-
assay at
25
-
-
assay at
25
-
Q40144, Q43527, Q43528, Q6RHX7, Q6RHX8, Q6RHX9, Q6RHY0, Q6RHY1, Q9FR51, Q9FZ05, Q9SDX0, Q9SLN9
xyloglucan endotransglucosylase assay; xyloglucan endotransglucosylase assay; xyloglucan endotransglucosylase assay; xyloglucan endotransglucosylase assay; xyloglucan endotransglucosylase assay; xyloglucan endotransglucosylase assay; xyloglucan endotransglucosylase assay; xyloglucan endotransglucosylase assay; xyloglucan endotransglucosylase assay; xyloglucan endotransglucosylase assay; xyloglucan endotransglucosylase assay; xyloglucan endotransglucosylase assay
25
-
Q9ZV40
XET activity assay
25
-
P93349
assay at
27
-
-
assay at
27
-
P24806
xyloglucan endotransglucosylase assay
28
-
-, Q9ZSU4
isoenzyme Meri-5
30
-
-
assay at
30
-
-
enzyme kinetics assay
30
-
-
XET activity assay
30
-
B1P1S7, -, P93671, P93672
assay at; assay at; assay at
37
-
-, Q9ZSU4
isoenzyme EXGT
37
-
-
assay at
additional information
-
-, Q9ZSU4
assay at room temperature
TEMPERATURE RANGE
TEMPERATURE MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
0
-
-
the enzyme also operates efficiently at 0 C, where it shows almost 40% of the activity observed at 30C
5
-
-, Q9ZSU4
all 4 isoenzymes are markedly cold-tolerant retaining activity at 5C; all 4 isoenzymes are markedly cold-tolerant retaining activity at 5C
10
50
-, Q6YDN9
about 20% of maximal activity at 10C and 50C, about 50% of maximal activity at 20C and 48C, temperature-profile
15
55
-
temperature-profile, about 20% of maximal activity at 15C and 55C
18
26
-
recombinant TCH4 protein, more active at lower temperatures, at or below 18C, less active at higher temperatures, above 26C
45
-
-, Q9ZSU4
isoenzymes EXGT and Meri-5 retain approximately 80% of maximum activity; isoenzymes TCH4 and XTR9 activities are reduced by 55-60%; isoenzymes TCH4 and XTR9 activities are reduced by 55-60%
pI VALUE
pI VALUE MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
4.4
9.6
-
at least 5 isozymes in cotyledons and leaves, development of a sensitive method for activity detection of isozymes in native isoelectric focusing gels, overview
4.6
-
C0IRG0, C0IRG2, C0IRH1
calculated from amino acid sequence
4.7
-
C0IRH4, C0IRH6, C0IRH7, C0IRH8, C0IRH9, C0IRI0, C0IRI1, C0IRI2, C0IRI3, C0IRI4, Q8GTJ0
calculated from amino acid sequence
4.8
-
-
native isoelectric focusing
4.8
-
C0IRH4, C0IRH6, C0IRH7, C0IRH8, C0IRH9, C0IRI0, C0IRI1, C0IRI2, C0IRI3, C0IRI4, Q8GTJ0
calculated from amino acid sequence
4.9
-
C0IRG8
calculated from amino acid sequence
5.3
-
C0IRG0, C0IRG2, C0IRH1
calculated from amino acid sequence
5.77
-
-
calculated pI-value
5.9
-
C0IRH4, C0IRH6, C0IRH7, C0IRH8, C0IRH9, C0IRI0, C0IRI1, C0IRI2, C0IRI3, C0IRI4, Q8GTJ0
calculated from amino acid sequence
5.96
-
-
calculated pI-value
6.1
-
C0IRG3, C0IRG4, C0IRG5, C0IRG6, C0IRG7, C0IRG9, C0IRH2
calculated from amino acid sequence
6.3
-
-
major enzyme form XET(6.3), isoelectric focusing
6.37
-
-
calculated pI-value
6.4
-
C0IRG3, C0IRG4, C0IRG5, C0IRG6, C0IRG7, C0IRG9, C0IRH2
calculated from amino acid sequence
6.6
-
-
about, isozymes sXET and eXET, isoelectric focusing
6.7
-
B1P1S7, P93668
isoelectric focusing; isoelectric focusing
6.7
-
C0IRG3, C0IRG4, C0IRG5, C0IRG6, C0IRG7, C0IRG9, C0IRH2
calculated from amino acid sequence
6.8
-
C0IRH4, C0IRH6, C0IRH7, C0IRH8, C0IRH9, C0IRI0, C0IRI1, C0IRI2, C0IRI3, C0IRI4, Q8GTJ0
calculated from amino acid sequence
6.9
-
C0IRH4, C0IRH6, C0IRH7, C0IRH8, C0IRH9, C0IRI0, C0IRI1, C0IRI2, C0IRI3, C0IRI4, Q8GTJ0
calculated from amino acid sequence
7.1
-
-
native isoelectric focusing
7.5
-
C0IRG3, C0IRG4, C0IRG5, C0IRG6, C0IRG7, C0IRG9, C0IRH2
calculated from amino acid sequence
7.7
-
C0IRH4, C0IRH6, C0IRH7, C0IRH8, C0IRH9, C0IRI0, C0IRI1, C0IRI2, C0IRI3, C0IRI4, Q8GTJ0
calculated from amino acid sequence
8
-
C0IRH0, C0IRH3
calculated from amino acid sequence
8
-
C0IRG3, C0IRG4, C0IRG5, C0IRG6, C0IRG7, C0IRG9, C0IRH2
calculated from amino acid sequence
8.2
-
C0IRH4, C0IRH6, C0IRH7, C0IRH8, C0IRH9, C0IRI0, C0IRI1, C0IRI2, C0IRI3, C0IRI4, Q8GTJ0
calculated from amino acid sequence
8.3
-
C0IRG3, C0IRG4, C0IRG5, C0IRG6, C0IRG7, C0IRG9, C0IRH2
calculated from amino acid sequence
8.3
-
C0IRH4, C0IRH6, C0IRH7, C0IRH8, C0IRH9, C0IRI0, C0IRI1, C0IRI2, C0IRI3, C0IRI4, Q8GTJ0
calculated from amino acid sequence
8.5
-
-
isoelectric focusing
8.5
-
-
2D-SDS-PAGE
8.7
-
C0IRH0, C0IRH3
calculated from amino acid sequence
9.2
-
C0IRG3, C0IRG4, C0IRG5, C0IRG6, C0IRG7, C0IRG9, C0IRH2
calculated from amino acid sequence
9.2
-
C0IRH4, C0IRH6, C0IRH7, C0IRH8, C0IRH9, C0IRI0, C0IRI1, C0IRI2, C0IRI3, C0IRI4, Q8GTJ0
calculated from amino acid sequence
9.4
-
Lactuca sativa ssp. capitata, Piper sp.
-
native isoelectric focusing
9.4
-
C0IRG0, C0IRG2, C0IRH1
calculated from amino acid sequence
9.8
-
C0IRH4, C0IRH6, C0IRH7, C0IRH8, C0IRH9, C0IRI0, C0IRI1, C0IRI2, C0IRI3, C0IRI4, Q8GTJ0
calculated from amino acid sequence
additional information
-
-
at least 4 isozymes, development of a sensitive method for activity detection of isozymes in native isoelectric focusing gels, overview
SOURCE TISSUE
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
SOURCE
-, Q5Z6G7, Q5Z6H1, Q5Z6H3, Q6ZAN9, Q763W5, Q76BW5, Q8VWG5
-
Manually annotated by BRENDA team
-
source for mRNA isolation
Manually annotated by BRENDA team
-
NXG1 is exclusively expressed in cotyledons
Manually annotated by BRENDA team
-
XET1 is highly expressed in young epicotyls
Manually annotated by BRENDA team
-
eXET, in epicotyls and other growing regions
Manually annotated by BRENDA team
C0IRG3, C0IRG4, C0IRG5, C0IRG6, C0IRG7, C0IRG9, C0IRH2
-
Manually annotated by BRENDA team
C0IRG0, C0IRG2, C0IRH1
-
Manually annotated by BRENDA team
C0IRH4, C0IRH6, C0IRH7, C0IRH8, C0IRH9, C0IRI0, C0IRI1, C0IRI2, C0IRI3, C0IRI4, Q8GTJ0
-
Manually annotated by BRENDA team
Dianthus caryophyllus Light Pink Barbara
-
-
-
Manually annotated by BRENDA team
Gossypium hirsutum Coker 312
-
-
-
Manually annotated by BRENDA team
-
core tissue of ripe kiwifruit
Manually annotated by BRENDA team
-
tXET-B1 is most abundant in pink fruit pericarp
Manually annotated by BRENDA team
Cucumis sativus, Piper sp.
-
-
Manually annotated by BRENDA team
C0IRH0, C0IRH3
ripe fruit; ripe fruit
Manually annotated by BRENDA team
C0IRG3, C0IRG4, C0IRG5, C0IRG6, C0IRG7, C0IRG9, C0IRH2
ripe fruit; ripe fruit; ripe fruit; ripe fruit; ripe fruit; ripe fruit; ripe fruit
Manually annotated by BRENDA team
C0IRG0, C0IRG2, C0IRH1
ripe fruit; ripe fruit; ripe fruit
Manually annotated by BRENDA team
C0IRG8
ripe fruit
Manually annotated by BRENDA team
C0IRG1
ripe fruit
Manually annotated by BRENDA team
C0IRH4, C0IRH6, C0IRH7, C0IRH8, C0IRH9, C0IRI0, C0IRI1, C0IRI2, C0IRI3, C0IRI4, Q8GTJ0
ripe fruit; ripe fruit; ripe fruit; ripe fruit; ripe fruit; ripe fruit; ripe fruit; ripe fruit; ripe fruit; ripe fruit; ripe fruit
Manually annotated by BRENDA team
Q40144
LeEXT gene is expressed in outer cell layers of the hypocotyl, after auxin treatment overlapping spatial distribution in the epidermis and outer cortical cell layers
Manually annotated by BRENDA team
Gossypium hirsutum Coker 312
-
-
-
Manually annotated by BRENDA team
-
internodes I, II, III and V, numbered from the cotyledonary node
Manually annotated by BRENDA team
-, Q5Z6G7, Q5Z6H1, Q5Z6H3, Q6ZAN9, Q763W5, Q76BW5, Q8VWG5
-
Manually annotated by BRENDA team
-
leaves from 5-day seedlings
Manually annotated by BRENDA team
-
XET1 is expressed in young and mature leaves at lower levels than in young epicotyls and roots, 7fold higher expression in young leaves than in mature ones
Manually annotated by BRENDA team
P93349
source leaf, levels of NtXET-1 mRNA decreases in midribs with increasing age of leaves
Manually annotated by BRENDA team
Q8GZD5
PttXET16A is expressed transiently in developing leaves
Manually annotated by BRENDA team
Allium ampeloprasum, Lactuca sativa ssp. capitata, Tropaeolum majus
-
-
Manually annotated by BRENDA team
C0IRH0, C0IRH3
;
Manually annotated by BRENDA team
C0IRG3, C0IRG4, C0IRG5, C0IRG6, C0IRG7, C0IRG9, C0IRH2
-
Manually annotated by BRENDA team
C0IRG0, C0IRG2, C0IRH1
-
Manually annotated by BRENDA team
C0IRH4, C0IRH6, C0IRH7, C0IRH8, C0IRH9, C0IRI0, C0IRI1, C0IRI2, C0IRI3, C0IRI4, Q8GTJ0
-
Manually annotated by BRENDA team
-
highest in green immature leaves as compared to maature leaves and brown, over-wintered leaves
Manually annotated by BRENDA team
D7URZ0, D7URZ1, D7US89, -, Q1PCS6
DcXTH2 transcript is detected in large quantities in petals as compared with other tissues; DcXTH3 transcript is detected in large quantities in petals as compared with other tissues
Manually annotated by BRENDA team
Dianthus caryophyllus Light Pink Barbara
-
DcXTH2 transcript is detected in large quantities in petals as compared with other tissues; DcXTH3 transcript is detected in large quantities in petals as compared with other tissues
-
Manually annotated by BRENDA team
Gossypium hirsutum Coker 312
-
-
-
Manually annotated by BRENDA team
Apium sp.
-
activity is very pronounced in the vicinity of the vascular bundles
Manually annotated by BRENDA team
Apium sp.
-
young petioles, high activity in thick-walled collenchyma cells
Manually annotated by BRENDA team
Q8GZD5
XET activity in xylem and phloem fibers at the stage of secondary wall formation, PttXET16A
Manually annotated by BRENDA team
-, Q5Z6G7, Q5Z6H1, Q5Z6H3, Q6ZAN9, Q763W5, Q76BW5, Q8VWG5
-
Manually annotated by BRENDA team
-
XET1 is highly expressed in young roots
Manually annotated by BRENDA team
Q8GZD5
PttXET16A expression in young roots and root tips
Manually annotated by BRENDA team
-
XET activity in the initiating root hairs; XET activity in the root cell elongation zone
Manually annotated by BRENDA team
-
XET activity in the root cell elongation zone
Manually annotated by BRENDA team
-, Q5Z6G7, Q5Z6H1, Q5Z6H3, Q6ZAN9, Q763W5, Q76BW5, Q8VWG5
-
Manually annotated by BRENDA team
Q5JZX2, -
elongation zone of the maize root
Manually annotated by BRENDA team
-
isozymes XTH14 and XTH26 are predominantly root-specific
Manually annotated by BRENDA team
C0IRH0, C0IRH3
;
Manually annotated by BRENDA team
C0IRG3, C0IRG4, C0IRG5, C0IRG6, C0IRG7, C0IRG9, C0IRH2
-
Manually annotated by BRENDA team
C0IRG0, C0IRG2, C0IRH1
-
Manually annotated by BRENDA team
C0IRH4, C0IRH6, C0IRH7, C0IRH8, C0IRH9, C0IRI0, C0IRI1, C0IRI2, C0IRI3, C0IRI4, Q8GTJ0
-
Manually annotated by BRENDA team
O80803, Q9FKL8, Q9FKL9, Q9M0D1, Q9M0D2
five predominantly root-expressed Arabidopsis thaliana XTHs belong to subgroup I/II, AtXTH12 is trichoblast-enriched; five predominantly root-expressed Arabidopsis thaliana XTHs belong to subgroup I/II, AtXTH13 is trichoblast-enriched; five predominantly root-expressed Arabidopsis thaliana XTHs belong to subgroup I/II, AtXTH17 is expressed in all cell types in the elongating and differentiating regions of the root; five predominantly root-expressed Arabidopsis thaliana XTHs belong to subgroup I/II, AtXTH18 is expressed in all cell types in the elongating and differentiating regions of the root; five predominantly root-expressed Arabidopsis thaliana XTHs belong to subgroup I/II, AtXTH19 is expressed in nearly all cell types in the root
Manually annotated by BRENDA team
Beckmannia syzigachne Fernald, Cedrus atlantica Carr., Crocus vernus Hill, Nicotiana tabacum SR-1, Pharbitis purpurea Bojer, Pinus parviflora Glauca, Trigonella caerulea Ser.
-
high XET activity in the epidermis cell wall of the elongation zone and in trichoblasts in the differentation zone
-
Manually annotated by BRENDA team
O80803, Q9FKL8, Q9FKL9, Q9M0D1, Q9M0D2
comparisons of mRNA populations of Arabidopsis root hair cells and cells of an Arabidopsis root hair mutant show AtXTH12 to be 4fold upregulated in root hair cells
Manually annotated by BRENDA team
-
cotyledons of germinated seeds
Manually annotated by BRENDA team
-
sXET, expressed during germination
Manually annotated by BRENDA team
-
expression of isozyme sXET during germination
Manually annotated by BRENDA team
-
7-8-day etiolated seedlings
Manually annotated by BRENDA team
Sinapis sp.
-
-
Manually annotated by BRENDA team
-
sprouting seedlings, more abundant in the growing tissues of the hypocotyls and leaves than in the cotyledons
Manually annotated by BRENDA team
B1P1S7, P93668
;
Manually annotated by BRENDA team
-
MXE, EC 2.4.1., and XET, actions are both detectable in living Equisetum fluviatile shoots, the MXE:XET ratio increasing with age
Manually annotated by BRENDA team
-
first year stem
Manually annotated by BRENDA team
-
etiolated stem, activity is positively correlated with growth rate in different zones of pea stem
Manually annotated by BRENDA team
-
etiolated 7-day stems
Manually annotated by BRENDA team
-
XET1 is expressed at lower levels in stems than in young epicotyls and roots
Manually annotated by BRENDA team
-
tXET-B1 is detected in stems
Manually annotated by BRENDA team
Q8GZD5
XET activity in xylem and phloem fibers at the stage of secondary wall formation, PttXET16A
Manually annotated by BRENDA team
-
developing, at least 16 Populus XTH genes are expressed in developing wood. Five genes are highly and ubiquitously expressed, whereas PtxtXET16-34 is expressed more weakly but specifically in developing wood; primary- and secondary-walled xylem tissues
Manually annotated by BRENDA team
Gossypium hirsutum Coker 312
-
-
-
Manually annotated by BRENDA team
additional information
-
XETs accumulates in expanding cells, at the sites of intercellular airspace, formation, and at the bases of leaves, cotyledons and hypocotyls, detailed localization
Manually annotated by BRENDA team
additional information
-
XET1 is not expressed in cotyledons
Manually annotated by BRENDA team
additional information
P93349
tissue-specific NtXET-1 expression pattern, highest mRNA levels in organs highly enriched in vascular tissues
Manually annotated by BRENDA team
additional information
Q8GZD5
detailed localization of XET in poplar stems, PttXET16A expression pattern, expression in secondary vascular tissues
Manually annotated by BRENDA team
additional information
-, Q5Z6G7, Q5Z6H1, Q5Z6H3, Q6ZAN9, Q763W5, Q76BW5, Q8VWG5
tissue-specific and growth stage-dependent isozyme expression patterns, negligible expression in shoot, overview; tissue-specific and growth stage-dependent isozyme expression patterns, negligible expression in shoot, overview; tissue-specific and growth stage-dependent isozyme expression patterns, negligible expression in shoot, overview; tissue-specific and growth stage-dependent isozyme expression patterns, negligible expression in shoot, overview; tissue-specific and growth stage-dependent isozyme expression patterns, negligible expression in shoot, overview; tissue-specific and growth stage-dependent isozyme expression patterns, negligible expression in shoot, overview; tissue-specific and growth stage-dependent isozyme expression patterns, negligible expression in shoot, overview; tissue-specific and growth stage-dependent isozyme expression patterns, negligible expression in shoot, overview
Manually annotated by BRENDA team
additional information
-
isozyme eXET also occurs in other growing regions
Manually annotated by BRENDA team
additional information
D7URZ0, D7URZ1, D7US89, -, Q1PCS6
transcript levels of XTH genes are regulaated in floral and vegetative tissues of carnation plants with opening flowers, quantitative tissse expression analysis by real-time RT-PCR; transcript levels of XTH genes are regulaated in floral and vegetative tissues of carnation plants with opening flowers, quantitative tissse expression analysis by real-time RT-PCR; transcript levels of XTH genes are regulated in floral and vegetative tissues of carnation plants with opening flowers, quantitative tissse expression analysis by real-time RT-PCR; transcript levels of XTH genes are regulated in floral and vegetative tissues of carnation plants with opening flowers, quantitative tissue expression analysis by real-time RT-PCR
Manually annotated by BRENDA team
additional information
-
total extracts of Equisetum arvense organs exhibit XET activity, but vegetative lateral shoot extracts of contain somewhat less XET activity
Manually annotated by BRENDA team
additional information
-
the three GhXTH genes are expressed differently with GhXTH1 predominantly expressed in elongating cotton fibers, overview. Almost no XTH expression in roots
Manually annotated by BRENDA team
additional information
Dianthus caryophyllus Light Pink Barbara
-
transcript levels of XTH genes are regulaated in floral and vegetative tissues of carnation plants with opening flowers, quantitative tissse expression analysis by real-time RT-PCR; transcript levels of XTH genes are regulaated in floral and vegetative tissues of carnation plants with opening flowers, quantitative tissse expression analysis by real-time RT-PCR; transcript levels of XTH genes are regulated in floral and vegetative tissues of carnation plants with opening flowers, quantitative tissse expression analysis by real-time RT-PCR; transcript levels of XTH genes are regulated in floral and vegetative tissues of carnation plants with opening flowers, quantitative tissue expression analysis by real-time RT-PCR
-
Manually annotated by BRENDA team
additional information
Gossypium hirsutum Coker 312
-
the three GhXTH genes are expressed differently with GhXTH1 predominantly expressed in elongating cotton fibers, overview. Almost no XTH expression in roots
-
Manually annotated by BRENDA team
LOCALIZATION
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
GeneOntology No.
LITERATURE
SOURCE
-
localized exclusively in
Manually annotated by BRENDA team
-
most of the XET activity is strongly associated with the cell wall
Manually annotated by BRENDA team
-
cell wall marker enzyme, XET is present in the cell walls in form of a competent glycosyl-enzyme complex
Manually annotated by BRENDA team
P93349
the same gene encodes a protein, that may be both soluble and bound to the cell wall
Manually annotated by BRENDA team
-, Q5Z6G7, Q5Z6H1, Q5Z6H3, Q6ZAN9, Q763W5, Q76BW5, Q8VWG5
-
Manually annotated by BRENDA team
Q5JZX2, -
ZmXTH1 is weakly bound to the cell wall
Manually annotated by BRENDA team
O80803, Q9FKL8, Q9FKL9, Q9M0D1, Q9M0D2
-
Manually annotated by BRENDA team
P93349
the same gene encodes a protein, that may be both soluble and bound to the cell wall
-
Manually annotated by BRENDA team
-
extracellular or apoplastic space of epicotyls
-
Manually annotated by BRENDA team
additional information
-
not ionically bound to the cell wall, little XET activity is covalently bound in the cell wall
-
Manually annotated by BRENDA team
additional information
-
during interphase EXGT is extensively secreted into the apoplast via the endoplasmic reticulum-Golgi apparatus network, during cytokinesis it is exclusively located in the phragmoblast and eventually transported to the cell plate
-
Manually annotated by BRENDA team
additional information
-
expression profiles of members of the XTH gene family, each XTH gene is likely to have a unique fingerprint of expression and regulation
-
Manually annotated by BRENDA team
additional information
-
-
-
Manually annotated by BRENDA team
additional information
P93349
the extensibility and xyloglucan endotransglucosylase activity in transgenic tomato plant hypocotyls is increased in the apical zone compared to the basal, overview
-
Manually annotated by BRENDA team
MOLECULAR WEIGHT
MOLECULAR WEIGHT MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
26500
-
-
deglycosylated major enzyme form XET(6.3), SDS-PAGE
29000
-
-
glycosylated major enzyme form XET(6.3), SDS-PAGE
29500
-
C0IRG3, C0IRG4, C0IRG5, C0IRG6, C0IRG7, C0IRG9, C0IRH2
calculated from amino acid sequence
29500
-
C0IRG8
calculated from amino acid sequence
29600
-
C0IRH4, C0IRH6, C0IRH7, C0IRH8, C0IRH9, C0IRI0, C0IRI1, C0IRI2, C0IRI3, C0IRI4, Q8GTJ0
calculated from amino acid sequence
29700
-
C0IRH4, C0IRH6, C0IRH7, C0IRH8, C0IRH9, C0IRI0, C0IRI1, C0IRI2, C0IRI3, C0IRI4, Q8GTJ0
calculated from amino acid sequence
29900
-
C0IRH4, C0IRH6, C0IRH7, C0IRH8, C0IRH9, C0IRI0, C0IRI1, C0IRI2, C0IRI3, C0IRI4, Q8GTJ0
calculated from amino acid sequence
30000
-
-
determined by SDS-PAGE and Western Blot analysis
30100
-
C0IRH4, C0IRH6, C0IRH7, C0IRH8, C0IRH9, C0IRI0, C0IRI1, C0IRI2, C0IRI3, C0IRI4, Q8GTJ0
calculated from amino acid sequence
30200
-
C0IRG3, C0IRG4, C0IRG5, C0IRG6, C0IRG7, C0IRG9, C0IRH2
calculated from amino acid sequence; calculated from amino acid sequence
30200
-
C0IRH4, C0IRH6, C0IRH7, C0IRH8, C0IRH9, C0IRI0, C0IRI1, C0IRI2, C0IRI3, C0IRI4, Q8GTJ0
calculated from amino acid sequence
30400
-
C0IRG0, C0IRG2, C0IRH1
calculated from amino acid sequence
31000
-
-
SDS-PAGE
31000
-
C0IRH4, C0IRH6, C0IRH7, C0IRH8, C0IRH9, C0IRI0, C0IRI1, C0IRI2, C0IRI3, C0IRI4, Q8GTJ0
calculated from amino acid sequence
31100
-
C0IRH0, C0IRH3
calculated from amino acid sequence
31200
-
C0IRG0, C0IRG2, C0IRH1
calculated from amino acid sequence
31730
-
-
calculated molecular mass
31800
-
C0IRG3, C0IRG4, C0IRG5, C0IRG6, C0IRG7, C0IRG9, C0IRH2
calculated from amino acid sequence; calculated from amino acid sequence
31800
-
C0IRG0, C0IRG2, C0IRH1
calculated from amino acid sequence
31800
-
C0IRH4, C0IRH6, C0IRH7, C0IRH8, C0IRH9, C0IRI0, C0IRI1, C0IRI2, C0IRI3, C0IRI4, Q8GTJ0
calculated from amino acid sequence
31900
-
C0IRG3, C0IRG4, C0IRG5, C0IRG6, C0IRG7, C0IRG9, C0IRH2
calculated from amino acid sequence
31900
-
C0IRH4, C0IRH6, C0IRH7, C0IRH8, C0IRH9, C0IRI0, C0IRI1, C0IRI2, C0IRI3, C0IRI4, Q8GTJ0
calculated from amino acid sequence
32000
-
-
SDS-PAGE
32000
-
C0IRH4, C0IRH6, C0IRH7, C0IRH8, C0IRH9, C0IRI0, C0IRI1, C0IRI2, C0IRI3, C0IRI4, Q8GTJ0
calculated from amino acid sequence
32200
-
Q5MD55
SDS-PAGE
32420
-
-
calculated molecular mass
32930
-
-
calculated molecular mass
33000
35000
B1P1S7, P93668
SDS-PAGE shows a single, but somewhat diffuse band of 33-35 kDa, indicating that the protein could be glycosylated; SDS-PAGE shows a single, but somewhat diffuse band of 33-35 kDa, indicating that the protein could be glycosylated
33400
-
Q5MB21
calculated from amino acid sequence
33780
-
-
mutant PttXET16-34 E85G, calculated molecular mass; mutant PttXET16-34 E85G, determined by ESI-TOF MS analysis
33790
-
-
mutant PttXET16-34 E85A, calculated molecular mass; mutant PttXET16-34 E85A, determined by ESI-TOF MS analysis
33810
-
-
mutant PttXET16-34 E85S, calculated molecular mass; mutant PttXET16-34 E85S, determined by ESI-TOF MS analysis
33850
-
-
PttXET16-34, calculated molecular mass, glycoform GlcNAc2Man8
34020
-
-
PttXET16-34, calculated molecular mass, glycoform GlcNAc2Man9
34180
-
-
PttXET16-34, calculated molecular mass, glycoform GlcNAc2Man10
35500
-
C0IRG3, C0IRG4, C0IRG5, C0IRG6, C0IRG7, C0IRG9, C0IRH2
calculated from amino acid sequence
36000
-
Q9ZV40
determined by SDS-PAGE
36000
-
C0IRH4, C0IRH6, C0IRH7, C0IRH8, C0IRH9, C0IRI0, C0IRI1, C0IRI2, C0IRI3, C0IRI4, Q8GTJ0
calculated from amino acid sequence
36700
-
C0IRH0, C0IRH3
calculated from amino acid sequence
37000
-
-
SDS-PAGE
38000
-
-
SDS-PAGE
59000
-
Q5JZX2, -
-
SUBUNITS
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
?
-
x * 31000, SDS-PAGE and calculated from the amino acid sequence, the cDNA encodes a 33.5 kDa precursor polypeptide, which is subsequently processed to a 31 kDa mature protein
?
-
x * 34000, predicted MW of the mature protein encoded by AdXET5 is 31.8 kDa, SDS-PAGE
?
-
x * 33400, predicted size of TCH4
?
-
x * 30460, tXET-B2; x * 30493, tXET-B1; x * 32000, His-tagged recombinant tXET-B2 with predicted molecular mass of 32 kDa
?
-
x * 32000, each isoenzyme, SDS-PAGE
?
P93349
x * 33900, calculated from the amino acid sequence; x * 36000, probably glycosylated enzyme, Western blot
?
-
x * 33000, SDS-PAGE
?
-, Q6YDN9
x * 33115, native enzyme, mass spectrometry, x * 31737, deglycosyated enzyme, mass spectrometry
POSTTRANSLATIONAL MODIFICATION
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
glycoprotein
-
AdXET5 and AdXET6: asparagine-linked carbohydrate, N-glycosylated, glycosylation of the protein is not required for endo-transglycosylase activity
proteolytic modification
-
21 amino acids signal peptide at the N-terminus is cleaved to produce the mature protein
glycoprotein
-, Q9ZSU4
all 4 isoenzymes, TCH4, Meri-5, EXGT and XTR9, are N-glycosylated, requirement of glycosylation for activity of the isoenzymes differs; all 4 isoenzymes, TCH4, Meri-5, EXGT and XTR9, are N-glycosylated, requirement of glycosylation for activity of the isoenzymes differs
proteolytic modification
-
mature TCH4 polypeptide lacks the putative N-terminal signal sequence
glycoprotein
-, Q6YDN9
one N-glycosylation site situated close to the predicted catalytic residues, enzyme is a high-mannose-type glycan, glycosylation is required for activity, deglycosylation results in 40% loss of activity, overview
glycoprotein
P93349
two putative glycosylation sites at amino acid positions 14 and 114
proteolytic modification
P93349
contains a hydrophobic N-terminus indicating a signal peptide
glycoprotein
-, Q5Z6G7, Q5Z6H1, Q5Z6H3, Q6ZAN9, Q763W5, Q76BW5, Q8VWG5
isozyme possesses an N-glycosylation site adjacent to the C-terminal side of the DEIDFEFLG motif; isozyme possesses an N-glycosylation site adjacent to the C-terminal side of the DEIDFEFLG motif; isozyme possesses an N-glycosylation site adjacent to the C-terminal side of the DEIDFEFLG motif; isozyme possesses an N-glycosylation site adjacent to the C-terminal side of the DEIDFEFLG motif; isozyme possesses an N-glycosylation site adjacent to the C-terminal side of the DEIDFEFLG motif; isozyme possesses an N-glycosylation site adjacent to the C-terminal side of the DEIDFEFLG motif; isozyme possesses an N-glycosylation site adjacent to the C-terminal side of the DEIDFEFLG motif; isozyme XTH1, not isozyme XTH21, possesses an N-glycosylation site adjacent to the C-terminal side of the DEIDFEFLG motif
no glycoprotein
-, Q5Z6G7, Q5Z6H1, Q5Z6H3, Q6ZAN9, Q763W5, Q76BW5, Q8VWG5
isozymes XTH19 and XTH20
glycoprotein
Q8GZD5
PttXET16A contains a putative N-glycosylation site
glycoprotein
-
enzyme contains a conserved N-glycosylation site situated proximal to the predicted catalytic site, deglycosylation by endoglycosidase H or by site-directed mutagenesis does not influence the enzyme activity
proteolytic modification
Q8GZD5
PttXET16A has a putative 22 amino acids signal peptide at the N-terminus
glycoprotein
-
tXET-B1 and tXET-B2 with N-glycosylation sites
proteolytic modification
-
tXET-B1 and tXET-B2 contain a hydrophobic signal peptide of 20 and 18 amino acids, respectively, typical of an exported protein
glycoprotein
-
-
no glycoprotein
-
-
proteolytic modification
-
hydrophobic core of 24 amino acids at the N-terminus, characteristic of a signal peptide, enzyme must be transported through the cell membrane in order to reach the cell wall, DNA encodes a 33.5 kDa precursor polypeptide, which is subsequently processed to a 31 kDa mature protein
glycoprotein
-
with mannosyl residues
glycoprotein
Q5JZX2, -
-
glycoprotein
O80803, Q9FKL8, Q9FKL9, Q9M0D1, Q9M0D2
AtXTH13 cDNA construct is correctly expressed and posttranslationally modified with variable N-linked glycans on one site
additional information
-, Q9ZSU4
co- and post-translational eucaryotic-specific modifications of TCH4 are critical for optimal XET activity
Crystallization/COMMENTARY
ORGANISM
UNIPROT ACCESSION NO.
LITERATURE
2 different crystal forms
-
purified recombinant enzyme, free enzyme or in complex with a xyloglucan nonasaccharide XLLG, hanging drop vapour diffusion method, 20C, 0.002 ml of 10 mg/ml protein in 10 mM HEPES, pH 7.0, mixed with equal volume of a solution containing 1.0 M NaOAc and 0.2 M imidazole, and again mixed with equal volume of reservoir solution containing 0.4 M potassium sodium tartrate, X-ray diffraction structure determination and analysis at 1.8 A resolution, overall structure is a beta-sandwich, molecular modeling
-
active-site loop deletion variant NXG1-DELTAYNIIG in free and product-complexed forms, using 0.2 M sodium formate, 0.1 M Bis Tris propane buffer at pH 6.5, and 20% (w/v) PEG 3350
-
pH STABILITY
pH STABILITY MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
3.5
4
-
at pH 3.5-4.0 no degradation is observed
5
7
-
the enzyme is 100% stable for 24 h in the pH range 5-7 at room temperature, but at pH values below and above this range 50-75% of the activity is lost
TEMPERATURE STABILITY
TEMPERATURE STABILITY MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
0
37
-
isozyme XTH14 shows high activity at 37C, and at least 80% of this activity is maintained down to 20C. At 4C the protein still displays more than 60% of ist activity at 37C. The activity abruptly decreases to about 30% at 0C. Isozyme XTH26 shows high activity at 37C, and at least 50% of this activity is maintained down to 20C. At 4C the protein still displays more than 30% of ist activity at 37C, while the activity abruptly decreases to about 15% at 0C
5
-
-, Q9ZSU4
all 4 isoenzymes are markedly cold-tolerant retaining activity at 5C; all 4 isoenzymes are markedly cold-tolerant retaining activity at 5C
38
50
-
the activity of XETs from nasturtium seeds is stable at 40C during 2 h (at 38C during 8 h), but it is completely lost at 50C
44
-
-
TCH4 protein: undetectable activity, more heat-sensitive than the total XET activity
52.3
-
-
unfolding temperature for the recombinant N93S mutant enzyme, slight protection by 20% glycerol or sucrose, 0.5 M urea, or NaCl at 350 mM, further destabilization by NaCl at 1 M
52.9
-
-
unfolding temperature for the recombinant wild-type enzyme, slight protection by 20% glycerol or sucrose, 0.5 M urea, or NaCl at 350 mM, further destabilization by NaCl at 1 M
95
-
B1P1S7, P93668
about 25% of isozyme XET6 activity survives incubation at 95C for 1 min
additional information
-
-
boiling inactivates
additional information
-
-
isoenzymes are heat-labile, none of the isoenzymes is particularly cold-tolerant
GENERAL STABILITY
ORGANISM
UNIPROT ACCESSION NO.
LITERATURE
loss of activity if frozen and stored at -80C
-
activity is lost after partial purification and desalting, ascorbic acid and 2-mercaptoethanol cannot prevent the loss, activity can be restored by addition of a wide variety of inorganic and organic salts
-
progressive purification destabilizes XET activity, 10% glycerol stabilizes
-
about 30% activity is lost during one freeze-thaw cycle
-, Q6YDN9
repeated cycles of freezing and thawing reduce its activity to around 10%
-
addition of bovine serum albumin does not affect enzyme activity or stability
-
0.1 mM final concentration spermine stabilizes
-
progressive purification destabilizes XET activity, 10% glycerol stabilizes
-
ORGANIC SOLVENT
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
Ethanol
-
15%, about 3 M, about 50% inhibition of product formation
Glycerol
-
the addition of 10% (v/v) glycerol has no affect on XET activity after several freeze-thawing cycles
STORAGE STABILITY
ORGANISM
UNIPROT ACCESSION NO.
LITERATURE
-80C, partially purified XET, ConA-eluate stage, 4 weeks, stable
-
-80C, purified XET, loss of activity
-
4C, purified XET, 24 h, stable
-
14C, 0.1 mg/ml enzyme in 50 mM ammonium acetate, pH 5.5, with 0.5 M ammonium sulfate, complete loss of activity within 3 months
-, Q6YDN9
-20C or -80C, activity declines gradually
-
-20C, 100 mM succinate or ammonium acetate buffers at pH 6.0 containing 5 mM calcium chloride, 1 year, no loss of activity
-
4C, at least 6 months, without loss of activity
-
Purification/COMMENTARY
ORGANISM
UNIPROT ACCESSION NO.
LITERATURE
His-Trap chelating column chromatography
C0IRH0, C0IRH3
3000fold, AdXET6
-
His-Trap chelating column chromatography; His-Trap chelating column chromatography
C0IRG3, C0IRG4, C0IRG5, C0IRG6, C0IRG7, C0IRG9, C0IRH2
ammonium sulfate precipitation, Hi-Trap SP Sepharose column chromatography, Superdex 75 gel filtration
-
partial, recombinant TCH4, Meri-5, EXGT and XTR9, expressed in Sf9 insect cells; partial, recombinant TCH4, Meri-5, EXGT and XTR9, expressed in Sf9 insect cells
-, Q9ZSU4
recombinant AtXTH12 partially from Pichia pastoris by ammonium sulfate fractionation; recombinant AtXTH13 partially from Pichia pastoris by ammonium sulfate fractionation, AtXTH13 is purified to apparent homogeneity by xylogluco-oligosaccharide affinity chromatography; recombinant AtXTH17 partially from Pichia pastoris by ammonium sulfate fractionation; recombinant AtXTH18 partially from Pichia pastoris by ammonium sulfate fractionation; recombinant AtXTH19 partially from Pichia pastoris by ammonium sulfate fractionation
O80803, Q9FKL8, Q9FKL9, Q9M0D1, Q9M0D2
recombinant TCH4 protein expressed in Escherichia coli
-
TCH4 and EXGT
-
Ni-substituted chelating Sepharose FF column chromatography
-
general, mechanism-based method for purification, about 200fold, step-wise addition of (NH4)2SO4 reveals distinct isoforms
-
native enzyme from florets to homogeneity by adsorption chromatography, 55-140fold purification dependent on different assay methods for quantification
-, Q6YDN9
direct elution from the acrylamide gel
-
ammonium sulfate precipitation, Sepharose Q column chromatography, phenyl Sepharose column chromatography and Bio-Gel P60 gel filtration; ammonium sulfate precipitation, Sepharose Q column chromatography, phenyl Sepharose column chromatography and Bio-Gel P60 gel filtration
B1P1S7, P93668
ammonium sulfate precipitation, Sepharose Q column chromatography, phenyl Sepharose column chromatography, chromatofocusing on PBE-94, and Bio-Gel P-60 gel filtration; Sepharose Q column chromatography, Phenyl-Sepharose chromatography, PBE-94 column chromatography and Bio-Gel P-60 gel filtration
-
immobilized metal affinity chromatography; immobilized metal affinity chromatography; immobilized metal affinity chromatography
B1P1S7, P93671, P93672
recombinant NtXET-1, expressed in Escherichia coli
P93349
protein extracts from bean cells are prepared
-
recombinant I, expressed in Pichia pastoris
-
recombinant enzyme from Pichia pastoris by strong cation exchange chromatography and gel filtration in a sequential combination
-
recombinant His6-tagged PttXET16A, expressed in Escherichia coli BL21(DE3)
Q8GZD5
recombinant wild-type and mutant enzymes from Pichia pastoris by ultrafiltration, one or two steps of cation exchange chromatography, dialysis, and adsorption chromatography
-
SP-Sepharose fast flow column chromatography and S-source 15S gel filtration
-
Supelclean ENVI-8 SPE column gel filtration
-
His-tagged recombinant tXET-B2, expressed in Escherichia coli
-
PD-10 column chromatography and UNO Q column chromatography
-
proteins are extracted from tomato plants; proteins are extracted from tomato plants; proteins are extracted from tomato plants; proteins are extracted from tomato plants; proteins are extracted from tomato plants; proteins are extracted from tomato plants; proteins are extracted from tomato plants; proteins are extracted from tomato plants; proteins are extracted from tomato plants; proteins are extracted from tomato plants; proteins are extracted from tomato plants; proteins are extracted from tomato plants
Q40144, Q43527, Q43528, Q6RHX7, Q6RHX8, Q6RHX9, Q6RHY0, Q6RHY1, Q9FR51, Q9FZ05, Q9SDX0, Q9SLN9
isozymes sXET and eXET from germinated seeds, i.e. cotyledons, and from epicotyls, respectively, to near homogeneity by ammonium sulfate fractionation, anion exchange chromatography, adsorption and desorption of the enzyme-substrate complex on cellulose, and hydrophobic-interaction chromatography
-
polyanion SI column chromatography, Spheron phosphate column chromatography, and Superdex 75 gel filtration
-
87fold, from apoplastic space of epicotyls
-
general, mechanism-based method for purification, about 200fold, step-wise addition of (NH4)2SO4 reveals distinct isoforms
-
-
Q5JZX2, -
Cloned/COMMENTARY
ORGANISM
UNIPROT ACCESSION NO.
LITERATURE
isoform XTH14 is expressed in Escherichia coli BL21 cells
C0IRH0, C0IRH3
6 isoforms, AdXET1-6 gene family is cloned, a full-length cDNA, AdXET5, is cloned, sequenced and overexpressed as active XET in Escherichia coli, purified XET is encoded by AdXET6
-
isoform XTH5 is expressed in Escherichia coli BL21 cells; isoform XTH7 is expressed in Escherichia coli BL21 cells
C0IRG3, C0IRG4, C0IRG5, C0IRG6, C0IRG7, C0IRG9, C0IRH2
3 insect-cell-produced XETs: EXGT, TCH4 and MERI-5
-
AtXTH12, DNA and amino acid sequence determination and analysis, expression in Pichia pastoris strain GS115; AtXTH13, DNA and amino acid sequence determination and analysis, expression in Pichia pastoris strain GS115. AtXTH13 cDNA construct is correctly expressed and posttranslationally modified with variable N-linked glycans on one site; AtXTH17, DNA and amino acid sequence determination and analysis, expression in Pichia pastoris strain GS115; AtXTH18, DNA and amino acid sequence determination and analysis, expression in Pichia pastoris strain GS115; AtXTH19, DNA and amino acid sequence determination and analysis, expression in Pichia pastoris strain GS115
O80803, Q9FKL8, Q9FKL9, Q9M0D1, Q9M0D2
AtXTH24 is expressed in baculovirus-infected insect cells
P24806
cloning and expression of TCH4 in Escherichia coli; TCH4 is a member of the XET-related gene family
-
cloning and expression of TCH4, Meri-5, EXGT and XTR9 using the baculovirus/Sf9 insect cell system, XTR9 is sequenced; cloning and expression of XTR9 using the baculovirus/Sf9 insect cell system, XTR9 is sequenced
-, Q9ZSU4
expressed in Pichia pastoris strain GS115
-
gene structure, genomic localization and phylogenetic relationship of the XTH gene family
-
into the pBIm and pCAM-BIA1300 vector, and into the pPIC6A expression vector for the production of recombinant AtXTH21 protein using the Pichia pastoris expression system
Q9ZV40
TCH4 is a member of the XET-related gene family
-
expressed in Pichia pastoris strain GS115
-
DNA and amino acid sequence determination and analysis, expression in Pichia pastoris resulting in a recombinant enzyme which is more highly mannosylated than the native enzyme without having influence on activity
-, Q6YDN9
expressed in Arabidopsis thaliana
Q5MD55
expressed in Arabidopsis thaliana
-
expressed in Escherichia coli strain BL21 (DE3)
-
gene DcXTH1, DNA and amino acid sequence determination and analysis, genetic structure, sequence comparisons, and quantitative tissse expression analysis by real-time RT-PCR; gene DcXTH2, DNA and amino acid sequence determination and analysis, genetic structure, sequence comparisons, and quantitative tissue expression analysis by real-time RT-PCR; gene DcXTH3, DNA and amino acid sequence determination and analysis, genetic structure, sequence comparisons, and quantitative tissse expression analysis by real-time RT-PCR; XTH gene DcXTH4, DNA and amino acid sequence determination and analysis, genetic structure, sequence comparisons, and quantitative tissse expression analysis by real-time RT-PCR
D7URZ0, D7URZ1, D7US89, -, Q1PCS6
into the pMD-18T vector
-
into the pMD-18T vector
-
genes GhXTH1, GhXTH2, and GhXTH3, DNA and amino acid sequence determination and analysis, genetic structure, expression analysis, recombinant overexpression of isozyme GHXTH1 in transgenic cotton plants using Agrobacterium tumefaciens transfection method under transcriptional control of the CaMV 35S promoter
-
expressed in Pichia pastoris; expressed in Pichia pastoris
B1P1S7, P93668
expressed in Pichia pastoris; expressed in Pichia pastoris; expressed in Pichia pastoris
B1P1S7, P93671, P93672
gene HvXET3, DNA and amino acid sequence determination and analysis, expression in Pichia pastoris; gene HvXET4, DNA and amino acid sequence determination and analysis, expression in Pichia pastoris; gene HvXET6, DNA and amino acid sequence determination and analysis, expression in Pichia pastoris
B1P1S7, -, P93671, P93672
expression in Solanum lycopersicum cv. Money Maker via transfection by Agrobacterium tumefaciens strain C58C1:pGV2260 under control of promoter CaMV-35S. Expression analysis of SlXTH1 tomato gene by real-time quantitative PCR from apical and basal hypocotyls segments of wild-type and transgenic tomato lines, and extensibility of cell walls from apical and basal hypocotyls segments of wild type and transgenic tomato lines
P93349
NtXET-1 is cloned and expressed in Escherichia coli, ORF encodes a 295 amino acids protein, cDNA is cloned in sense and antisense orientation, creation of transgenic tobacco plants with reduced NtXET-1 expression: 2 independent lines with reduced total XET activity by 56% and 37%, respectively, in midribs of tobacco plants transformed with an antisense construct, which exhibit an at least 20% increase in the average MW of xyloglucan
P93349
DNA and amino acid sequence determination and analysis, phylogenetic analysis and sequence alignment; DNA and amino acid sequence determination and analysis, phylogenetic analysis and sequence alignment; DNA and amino acid sequence determination and analysis, phylogenetic analysis and sequence alignment; DNA and amino acid sequence determination and analysis, phylogenetic analysis and sequence alignment; DNA and amino acid sequence determination and analysis, phylogenetic analysis and sequence alignment; DNA and amino acid sequence determination and analysis, phylogenetic analysis and sequence alignment; DNA and amino acid sequence determination and analysis, phylogenetic analysis and sequence alignment; DNA and amino acid sequence determination and analysis, phylogenetic analysis and sequence alignment
-, Q5Z6G7, Q5Z6H1, Q5Z6H3, Q6ZAN9, Q763W5, Q76BW5, Q8VWG5
recombinant I expression in Pichia pastoris
-
DNA sequence determination and analysis, functional expression in Pichia pastoris
-
DNA sequence determination and analysis, methanol-induced, functional expression of wild-type and N93S mutant enzymes in Pichia pastoris, the recombinant wild-type enzyme is heterogeneous due to presence of variable N-glycosylation and incomplete cleavage of the alpha-factor secretion signal peptide
-
expressed in Pichia pastoris
-
expressed in Pichia pastoris strain GS115
-
for expression in Pichia pastoris strain GS115
-
phylogenetic tree and expression analysis of XTH genes, transgenic overexpression of PtxtXET16-34 in Populus tremula x tremuloides
-
PttXET16-34 is cloned and recombinantly expressed in Pichia pastoris
-
PttXET16A, a XET isoform in secondary vascular tissues and a member of XET subfamily I, is cloned, sequenced and expressed in Escherichia coli BL21(DE3), structure of the coding sequence
Q8GZD5
DNA and amino acid seuence determination and analysis, the poplar genome contains 40 XTH genes, overview
-
expressed in Pichia pastoris
-
LeEXT gene encoding XET is cloned
Q40144
small multi-gene family encodes XET: tXETB1, -B2, -B3 and -B4, tXET-B2 is cloned, sequenced and overexpressed in Escherichia coli, nucleotide and amino acid sequence of tXET-B1
-
expressed in Pichia pastoris strain GS115
-
genes XET1 and NXG1 encode 2 enzyme forms, XET1 cDNA is cloned and sequenced, amino acid sequence of NGX1
-
single copy gene, cDNA encoding XET is cloned, sequenced and encodes a 33.5 kDa precursor polypeptide, which is subsequently processed to a 31 kDa mature protein, NXG1 and 2 may be different alleles of the XET gene
-
into the pET28a+ vector for transformation of Escherichia coli BL21 cells, into the pPICZalphaA vector for transformation of Pichia pastoris X-33 cells, into the pCAMBIA1302 and pCAMBIA1300 vectors for transformation of onion and Arabidopsis thaliana cells, respectively
Q5JZX2, -
EXPRESSION
ORGANISM
UNIPROT ACCESSION NO.
LITERATURE
XTH expression responds to phytohormone treatments, treatments with brassinoids, gibberellic acid, and, naphthalene acetic acid all increase the expression of GhXTH1 transcripts in 15 DPA fibers, overview
-
XTH expression responds to phytohormone treatments, treatments with brassinoids, gibberellic acid, and, naphthalene acetic acid all increase the expression of GhXTH1 transcripts in 15 DPA fibers, overview
Gossypium hirsutum Coker 312
-
-
isozyme XTH7 is expressed at the red ripe stage; the expression of isozyme XTH5 is clearly associated with fruit ripening; the expression of isozyme XTH8 is clearly associated with fruit ripening; XTH12 is expressed at the red ripe stage; XTH1 is expressed at the red ripe stage; XTH2 is expressed at the red ripe stage; XTH3 is expressed at the red ripe stage; XTH4 is expressed at the red ripe stage; XTH6 is expressed at the red ripe stage; XTH9 is expressed at the red ripe stage
Q40144, Q6RHX7, Q6RHX8, Q6RHX9, Q6RHY0, Q6RHY1, Q9FR51, Q9FZ05, Q9SDX0, Q9SLN9
ENGINEERING
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
E97Q
-
At-XTH22: a Glu to Gln substitution, converting the active site sequence DEIDFEFL to DQIDFEFL, abolishes XET activity
E85A
-
site-directed mutagenesis of the catalytic nucleophile Glu-85 yields an enzyme with glycosynthase activity
E85G
-
PttXET16-34 catalytic nucleophile variant
E85G
-
site-directed mutagenesis of the catalytic nucleophile Glu-85 yields an enzyme with glycosynthase activity
E85S
-
site-directed mutagenesis of the catalytic nucleophile Glu-85 yields an enzyme with glycosynthase activity
N93S
-
site-directed mutagenesis, activity and thermal stability of the mutant enzyme are similar to the wild-type enzyme, but the mutant shows reduced solubility